CmaCh20G004700 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh20G004700
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	(R)-specific enoyl-CoA hydratase
Location	Cma_Chr20 : 2271624 .. 2272942 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAAGTCTGCGAGGAAACGACACATCTTCGGTTATCTTACTGAGAGTATAACAGCTCAGCTTCCCTCCATCTTTCAGTGGACCCATTTATTCAATACAACTAAAGGGCAACAGAATTTCTCATAATCGTTGTAATTACAGGTAATCTGCTTTCTCTTACTTTCCAAATTTGTTCAGTTCTCCCTTTGTTTTTTGAATCCTCGCTTTGCCCACAAGGTGTTTGATGAAATGCCAAACCCAAGTTTTAGTGACTTCGTTGAGCATCCTGGTAGTACAAACAAACACTTCTGAACATTCTTTTCTCTAGTGCTTTTAATGGCTGCCCTTTGTTCTCTTGTTACAGGCTTGTTTGTGGGAGACATTTGACTGATACTTATAGACCCTTTACAAGTATGCTAAGTAGGGGAGGTTTAATCTCATCCTATATTTCTTCTTCTTTGAAATGCTTCTCTTCTTCAACTTCTAATGTGCTCAAAGTTGGAGATATCTTGAGCCATACCAGAATCTTCACCAGTGAAGATGTTTTGGAATACTCCAAAGTGAGTCATGACTCAAATCCTCTGCACTTTGATTCTGCATTAGCCCGACGTGCTGGATTCGACGATCGTCTTGTTCATGGGATGCTTGTTGCATCAATGTTCCCTCGTGTCATTTCCTCTCATTTTGTAAGTCTAGATAGTCTGCATTGCTTACACACAACTTTATGGGGACTGAAAAATGGCACTCACGCTTGTTTGAGATCTTTTAATGTATGTGGATGATTGTAGCCAGGAGCCATCTATGTGTCACAAAGCTTGAATTTTAAGTTACCAGTCTACGTCGGAGAGACGATCATTGTTCGGGTTGAAGCAATTGACTTGAGAGAAATTAAGAAGAGATACCTGTAAGCTTGTTCTTGGCAAATCTCTACCTTCTCATTCGAATTTCAAGTTGTCATTTCATTGAGTTGGTGCTTGCTGAATGTGTTTCAGGGCGAAATTCCAAACGAAATGCTTGAGGAATGGCGACGAGCTTGTTCTGGATGGTGAGGCAAGGGCCATATTACCTTCTGATTTTTGTTCCACAGATGGTTAGACGATGATCGTCTAAGTCTCGACCGCTAGATGTGGTTGAAGGAGCAAAGTTTTCCATATGAATTCATTCAATTTCTTCTTTGCTCTCTGTGGATAAAGATTGTCAAGTCTGGGAATAGGTCTGACCTCTATTTGCTTTTACTTCATCTCTTATTGATAGGTTATGAACAATATCTGGACCTGTTTCTTTCAATAAATTTGCCATGAGCCCTATGATCTTGGAACTGTTCTATCAATAGAGACTAAC

mRNA sequence

GGAAGTCTGCGAGGAAACGACACATCTTCGGTTATCTTACTGAGAGTATAACAGCTCAGCTTCCCTCCATCTTTCAGTGGACCCATTTATTCAATACAACTAAAGGGCAACAGAATTTCTCATAATCGTTGTAATTACAGGCTTGTTTGTGGGAGACATTTGACTGATACTTATAGACCCTTTACAAGTATGCTAAGTAGGGGAGGTTTAATCTCATCCTATATTTCTTCTTCTTTGAAATGCTTCTCTTCTTCAACTTCTAATGTGCTCAAAGTTGGAGATATCTTGAGCCATACCAGAATCTTCACCAGTGAAGATGTTTTGGAATACTCCAAAGTGAGTCATGACTCAAATCCTCTGCACTTTGATTCTGCATTAGCCCGACGTGCTGGATTCGACGATCGTCTTGTTCATGGGATGCTTGTTGCATCAATGTTCCCTCGTGTCATTTCCTCTCATTTTCCAGGAGCCATCTATGTGTCACAAAGCTTGAATTTTAAGTTACCAGTCTACGTCGGAGAGACGATCATTGTTCGGGTTGAAGCAATTGACTTGAGAGAAATTAAGAAGAGATACCTGGCGAAATTCCAAACGAAATGCTTGAGGAATGGCGACGAGCTTGTTCTGGATGGTGAGGCAAGGGCCATATTACCTTCTGATTTTTGTTCCACAGATGGTTAGACGATGATCGTCTAAGTCTCGACCGCTAGATGTGGTTGAAGGAGCAAAGTTTTCCATATGAATTCATTCAATTTCTTCTTTGCTCTCTGTGGATAAAGATTGTCAAGTCTGGGAATAGGTCTGACCTCTATTTGCTTTTACTTCATCTCTTATTGATAGGTTATGAACAATATCTGGACCTGTTTCTTTCAATAAATTTGCCATGAGCCCTATGATCTTGGAACTGTTCTATCAATAGAGACTAAC

Coding sequence (CDS)

ATGCTAAGTAGGGGAGGTTTAATCTCATCCTATATTTCTTCTTCTTTGAAATGCTTCTCTTCTTCAACTTCTAATGTGCTCAAAGTTGGAGATATCTTGAGCCATACCAGAATCTTCACCAGTGAAGATGTTTTGGAATACTCCAAAGTGAGTCATGACTCAAATCCTCTGCACTTTGATTCTGCATTAGCCCGACGTGCTGGATTCGACGATCGTCTTGTTCATGGGATGCTTGTTGCATCAATGTTCCCTCGTGTCATTTCCTCTCATTTTCCAGGAGCCATCTATGTGTCACAAAGCTTGAATTTTAAGTTACCAGTCTACGTCGGAGAGACGATCATTGTTCGGGTTGAAGCAATTGACTTGAGAGAAATTAAGAAGAGATACCTGGCGAAATTCCAAACGAAATGCTTGAGGAATGGCGACGAGCTTGTTCTGGATGGTGAGGCAAGGGCCATATTACCTTCTGATTTTTGTTCCACAGATGGTTAG

Protein sequence

MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGFDDRLVHGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAIDLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSDFCSTDG

Homology

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BLAST of CmaCh20G004700 vs. Swiss-Prot
Match: PHAJ_AERCA ((R)-specific enoyl-CoA hydratase OS=Aeromonas caviae GN=phaJ PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 3.7e-18
Identity = 52/131 (39.69%), Postives = 75/131 (57.25%), Query Frame = 1


Query: 27  LKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGFDDRLVHGMLVASMFPRV 86
           L+VG     ++ F + +V  ++ +S D NPLH D A A    F+  +VHGML+AS+F  +
Sbjct: 6   LEVGQKARLSKRFGAAEVAAFAALSEDFNPLHLDPAFAATTAFERPIVHGMLLASLFSGL 65

Query: 87  ISSHFP--GAIYVSQSLNFKLPVYVGETIIVRVEAIDLREIKKRYLAKFQTKCLRNGDEL 146
           +    P  G+IY+ QSL+FKLPV+VG+ +   VE   LRE K   +A   T+    G  L
Sbjct: 66  LGQQLPGKGSIYLGQSLSFKLPVFVGDEVTAEVEVTALREDKP--IATLTTRIFTQGGAL 125

Query: 147 VLDGEARAILP 156
            + GEA   LP
Sbjct: 126 AVTGEAVVKLP 134

[Close]

BLAST of CmaCh20G004700 vs. Swiss-Prot
Match: CROR_METEA (3-hydroxybutyryl-CoA dehydratase OS=Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1) GN=croR PE=4 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.0e-16
Identity = 45/118 (38.14%), Postives = 71/118 (60.17%), Query Frame = 1


Query: 40  TSEDVLEYSKVSHDSNPLHFDSALARRAGFDDRLVHGMLVASMFPRVISSHF--PGAIYV 99
           +S DV+ +++++ D NP+H     A R  F  R+ HG+  A +   V+ +    PGA+Y+
Sbjct: 26  SSSDVVGFAEITGDRNPIHLSEHFAARTPFGTRIAHGLYTAGLISAVLGTRLPGPGAVYI 85

Query: 100 SQSLNFKLPVYVGETIIVRVEAIDLREIKKRYLAKFQTKCLRNGDELVLDGEARAILP 156
           SQ+LNF+ PV +G+ + V+VE  +L  I +R  A+    C   GDE+VLDGEA   +P
Sbjct: 86  SQTLNFRAPVRIGDIVEVKVEVAEL--IPERRRARLACTC-SVGDEVVLDGEALVKVP 140

[Close]

BLAST of CmaCh20G004700 vs. Swiss-Prot
Match: PHAJ_RHORT ((R)-specific enoyl-CoA hydratase OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1) GN=phaJ PE=1 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 3.2e-14
Identity = 44/132 (33.33%), Postives = 72/132 (54.55%), Query Frame = 1


Query: 27  LKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGFDDRLVHGMLVASMFPRV 86
           +K G   S  +  +  D+  ++ +S D+NP H +   A+   F  R+ HGML A     V
Sbjct: 14  IKEGQSASLAKTISESDIYLFAGLSMDTNPAHVNEDYAQTTVFKTRIAHGMLSAGFISAV 73

Query: 87  ISSHFPG--AIYVSQSLNFKLPVYVGETIIVRVEAIDLREIKKRYLAKFQTKCLRNGDEL 146
           + +  PG  AIYV+QSL FK PV +G+T+   V    L  + ++    F+T C   G ++
Sbjct: 74  LGTRLPGPGAIYVNQSLKFKAPVRIGDTVTATVTVTGL--VPEKKFVTFRTTCTVAG-KV 133

Query: 147 VLDGEARAILPS 157
           V++GEA  ++P+
Sbjct: 134 VIEGEATVMVPA 142

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BLAST of CmaCh20G004700 vs. Swiss-Prot
Match: HTD2_HUMAN (Hydroxyacyl-thioester dehydratase type 2, mitochondrial OS=Homo sapiens GN=RPP14 PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 9.4e-14
Identity = 39/132 (29.55%), Postives = 72/132 (54.55%), Query Frame = 1


Query: 27  LKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGFDDRLVHGMLVASMFPRV 86
           +KVGD     R FT  DV  +S+++ D NPLH +   A+   F + +VHG+L+  +   +
Sbjct: 36  IKVGDRAELRRAFTQTDVATFSELTGDVNPLHLNEDFAKHTKFGNTIVHGVLINGLISAL 95

Query: 87  ISSHFPG--AIYVSQSLNFKLPVYVGETIIVRVEAIDLREIKKRYLAKFQTKC-LRNGDE 146
           + +  PG   +++SQ ++F  P+Y+GE ++   E   L    KR++A     C +    +
Sbjct: 96  LGTKMPGPGCVFLSQEISFPAPLYIGEVVLASAEVKKL----KRFIAIIAVSCSVIESKK 155

Query: 147 LVLDGEARAILP 156
            V++G  + ++P
Sbjct: 156 TVMEGWVKVMVP 163

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BLAST of CmaCh20G004700 vs. TrEMBL
Match: A0A0A0KCN3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G290840 PE=4 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 6.7e-67
Identity = 135/162 (83.33%), Postives = 150/162 (92.59%), Query Frame = 1


Query: 1   MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLS+G L S+YISSSLKCFSSSTS+VLKVGDILS+ RIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSKG-LFSTYISSSLKCFSSSTSSVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SALARRAGFDDRLVHGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120
           S LA+RAGF+  LVHG+LVASMFP +ISSH+PGAIYVSQSLNFKLPVYVGE I+ +VEAI
Sbjct: 61  SKLAQRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAI 120

Query: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSDFCSTD 163
           +LRE KKRYLAKF+TKCLRNGDELVL+GEARAILP DF ST+
Sbjct: 121 ELRENKKRYLAKFKTKCLRNGDELVLEGEARAILPVDFHSTN 161

[Close]

BLAST of CmaCh20G004700 vs. TrEMBL
Match: A0A067G0J2_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g031503mg PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 1.0e-51
Identity = 99/141 (70.21%), Postives = 123/141 (87.23%), Query Frame = 1


Query: 16  LKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGFDDRLVH 75
           L+ FSS    +LK GDIL  TRIF+SEDV+EYSKVSHDSNPLHF+S  AR AGFDDRLVH
Sbjct: 14  LRYFSSLEPRILKTGDILRQTRIFSSEDVVEYSKVSHDSNPLHFNSESARNAGFDDRLVH 73

Query: 76  GMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAIDLREIKKRYLAKFQT 135
           GMLVASMFP++ISSHFPGA+YVSQSL+F+LPVY+G+ ++ +++A+++RE+KKRYL KF T
Sbjct: 74  GMLVASMFPQIISSHFPGAVYVSQSLHFRLPVYIGDEVLGQLQAVNVREMKKRYLVKFST 133

Query: 136 KCLRNGDELVLDGEARAILPS 157
           KC++NG+ LVLDGEA A LPS
Sbjct: 134 KCIKNGELLVLDGEAMAFLPS 154

[Close]

BLAST of CmaCh20G004700 vs. TrEMBL
Match: W9RN50_9ROSA ((R)-specific enoyl-CoA hydratase OS=Morus notabilis GN=L484_014415 PE=4 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 1.8e-48
Identity = 100/156 (64.10%), Postives = 124/156 (79.49%), Query Frame = 1


Query: 1   MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           ML R  L SS+I S L+CFSSS   +LKVGD L  TR F  ED++EY+KV++DSNP+HFD
Sbjct: 1   MLIRNLLSSSHIPS-LRCFSSSAPCLLKVGDTLKQTRTFADEDLMEYAKVTYDSNPIHFD 60

Query: 61  SALARRAGFDDRLVHGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120
           S  AR AGF+DRLVHG LVASM P +ISSHFPGA+Y+SQS++FKLPVY+GE I   +EAI
Sbjct: 61  SEAARNAGFEDRLVHGTLVASMIPHIISSHFPGAVYLSQSVHFKLPVYIGEEIAAEMEAI 120

Query: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPS 157
           ++R  KKRY+AK + +C +NG+ LVLDGEA AILPS
Sbjct: 121 NIRANKKRYIAKLKIRCFKNGELLVLDGEAMAILPS 155

[Close]

BLAST of CmaCh20G004700 vs. TrEMBL
Match: A0A0B2R270_GLYSO ((R)-specific enoyl-CoA hydratase OS=Glycine soja GN=glysoja_038244 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 3.1e-48
Identity = 96/144 (66.67%), Postives = 118/144 (81.94%), Query Frame = 1


Query: 13  SSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGFDDR 72
           S SL+CF+S T NVLK GD+L   R++T EDVL+YSKVS DSNPLH DSA A+  GF+  
Sbjct: 13  SLSLRCFTSGTPNVLKPGDVLRKARVYTDEDVLQYSKVSCDSNPLHTDSAAAKDVGFEGP 72

Query: 73  LVHGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAIDLREIKKRYLAK 132
           LVHGMLVAS+FP +ISSHFPGA+YVSQ+LNFK PVY+G+ II  V+A +LRE K RYLAK
Sbjct: 73  LVHGMLVASLFPHIISSHFPGAVYVSQNLNFKFPVYIGDQIIGEVQATNLRENKNRYLAK 132

Query: 133 FQTKCLRNGDELVLDGEARAILPS 157
           F+T+C +NGD LV+DGEA A+LP+
Sbjct: 133 FKTRCFKNGDLLVIDGEAVALLPT 156

[Close]

BLAST of CmaCh20G004700 vs. TrEMBL
Match: I1M7A1_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_14G041000 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 3.1e-48
Identity = 96/144 (66.67%), Postives = 118/144 (81.94%), Query Frame = 1


Query: 13  SSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGFDDR 72
           S SL+CF+S T NVLK GD+L   R++T EDVL+YSKVS DSNPLH DSA A+  GF+  
Sbjct: 13  SLSLRCFTSGTPNVLKPGDVLRKARVYTDEDVLQYSKVSCDSNPLHTDSAAAKDVGFEGP 72

Query: 73  LVHGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAIDLREIKKRYLAK 132
           LVHGMLVAS+FP +ISSHFPGA+YVSQ+LNFK PVY+G+ II  V+A +LRE K RYLAK
Sbjct: 73  LVHGMLVASLFPHIISSHFPGAVYVSQNLNFKFPVYIGDQIIGEVQATNLRENKNRYLAK 132

Query: 133 FQTKCLRNGDELVLDGEARAILPS 157
           F+T+C +NGD LV+DGEA A+LP+
Sbjct: 133 FKTRCFKNGDLLVIDGEAVALLPT 156

[Close]

BLAST of CmaCh20G004700 vs. TAIR10
Match: AT5G60335.1 (AT5G60335.1 Thioesterase superfamily protein)

HSP 1 Score: 184.1 bits (466), Expect = 6.8e-47
Identity = 90/143 (62.94%), Postives = 117/143 (81.82%), Query Frame = 1


Query: 16  LKCFSSSTSN-VLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGFDDRLV 75
           L+ FSS  S  +LKVGD+L  TR+F+SED+  Y++VSHD NPLHFD   AR+AGF++RLV
Sbjct: 14  LRSFSSVASKTLLKVGDVLRETRVFSSEDIKAYAEVSHDWNPLHFDPESARKAGFENRLV 73

Query: 76  HGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAIDLREIKKRYLAKFQ 135
           HGMLV+SMFPR+IS+HFPGA+YVSQSL+F+ PVY+G+ I+  V+AI LRE K +Y+ KF 
Sbjct: 74  HGMLVSSMFPRIISAHFPGAVYVSQSLHFRSPVYIGDEILGLVQAIALRETKNKYIVKFS 133

Query: 136 TKCLRNGDEL-VLDGEARAILPS 157
           TKC +N +EL V+DGEA AILP+
Sbjct: 134 TKCFKNHNELVVIDGEATAILPN 156

[Close]

BLAST of CmaCh20G004700 vs. NCBI nr
Match: gi|449463667|ref|XP_004149553.1| (PREDICTED: hydroxyacyl-thioester dehydratase type 2, mitochondrial [Cucumis sativus])

HSP 1 Score: 261.5 bits (667), Expect = 9.6e-67
Identity = 135/162 (83.33%), Postives = 150/162 (92.59%), Query Frame = 1


Query: 1   MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLS+G L S+YISSSLKCFSSSTS+VLKVGDILS+ RIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSKG-LFSTYISSSLKCFSSSTSSVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SALARRAGFDDRLVHGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120
           S LA+RAGF+  LVHG+LVASMFP +ISSH+PGAIYVSQSLNFKLPVYVGE I+ +VEAI
Sbjct: 61  SKLAQRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAI 120

Query: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSDFCSTD 163
           +LRE KKRYLAKF+TKCLRNGDELVL+GEARAILP DF ST+
Sbjct: 121 ELRENKKRYLAKFKTKCLRNGDELVLEGEARAILPVDFHSTN 161

[Close]

BLAST of CmaCh20G004700 vs. NCBI nr
Match: gi|659127833|ref|XP_008463911.1| (PREDICTED: hydroxyacyl-thioester dehydratase type 2, mitochondrial [Cucumis melo])

HSP 1 Score: 260.0 bits (663), Expect = 2.8e-66
Identity = 135/162 (83.33%), Postives = 149/162 (91.98%), Query Frame = 1


Query: 1   MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLSRG L S++ISSSLKCFSSSTSNVLKVGDILS+ RIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 45  MLSRG-LFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFD 104

Query: 61  SALARRAGFDDRLVHGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120
             LARRAGF+  LVHG+LVASMFP +ISSH+PGAIYVSQSLNFKLPVYVGE I+ +VEAI
Sbjct: 105 PELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAI 164

Query: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSDFCSTD 163
           +LRE KKRYLAKF+TKCLRNGDELVL+GEARAILP+ F ST+
Sbjct: 165 ELRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 205

[Close]

BLAST of CmaCh20G004700 vs. NCBI nr
Match: gi|568869910|ref|XP_006488157.1| (PREDICTED: (R)-specific enoyl-CoA hydratase [Citrus sinensis])

HSP 1 Score: 211.1 bits (536), Expect = 1.5e-51
Identity = 99/141 (70.21%), Postives = 123/141 (87.23%), Query Frame = 1


Query: 16  LKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGFDDRLVH 75
           L+ FSS    +LK GDIL  TRIF+SEDV+EYSKVSHDSNPLHF+S  AR AGFDDRLVH
Sbjct: 14  LRYFSSLEPRILKTGDILRQTRIFSSEDVVEYSKVSHDSNPLHFNSESARNAGFDDRLVH 73

Query: 76  GMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAIDLREIKKRYLAKFQT 135
           GMLVASMFP++ISSHFPGA+YVSQSL+F+LPVY+G+ ++ +++A+++RE+KKRYL KF T
Sbjct: 74  GMLVASMFPQIISSHFPGAVYVSQSLHFRLPVYIGDEVLGQLQAVNVREMKKRYLVKFST 133

Query: 136 KCLRNGDELVLDGEARAILPS 157
           KC++NG+ LVLDGEA A LPS
Sbjct: 134 KCIKNGELLVLDGEAMAFLPS 154

[Close]

BLAST of CmaCh20G004700 vs. NCBI nr
Match: gi|828296228|ref|XP_004491188.2| (PREDICTED: hydroxyacyl-thioester dehydratase type 2, mitochondrial [Cicer arietinum])

HSP 1 Score: 207.6 bits (527), Expect = 1.6e-50
Identity = 97/142 (68.31%), Postives = 120/142 (84.51%), Query Frame = 1


Query: 15  SLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGFDDRLV 74
           SL+CFSS+T +VLK GDIL  TR+FT EDVL+YSKVSHDSNPLH DSA AR  GF+  LV
Sbjct: 15  SLRCFSSATPHVLKPGDILKKTRVFTEEDVLQYSKVSHDSNPLHIDSATARNVGFEGPLV 74

Query: 75  HGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAIDLREIKKRYLAKFQ 134
           HGMLVAS+FP +ISSHFP A+YVSQ+LNFKLPVY+G+ I+  V+A +LRE K RYLAKF+
Sbjct: 75  HGMLVASLFPHIISSHFPAAVYVSQTLNFKLPVYIGDQIVAEVQATNLRENKNRYLAKFK 134

Query: 135 TKCLRNGDELVLDGEARAILPS 157
           T+C +NG+ LV++GEA A+LP+
Sbjct: 135 TRCFKNGEILVIEGEAMALLPT 156

[Close]

BLAST of CmaCh20G004700 vs. NCBI nr
Match: gi|703128568|ref|XP_010104130.1| ((R)-specific enoyl-CoA hydratase [Morus notabilis])

HSP 1 Score: 200.3 bits (508), Expect = 2.6e-48
Identity = 100/156 (64.10%), Postives = 124/156 (79.49%), Query Frame = 1


Query: 1   MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           ML R  L SS+I S L+CFSSS   +LKVGD L  TR F  ED++EY+KV++DSNP+HFD
Sbjct: 1   MLIRNLLSSSHIPS-LRCFSSSAPCLLKVGDTLKQTRTFADEDLMEYAKVTYDSNPIHFD 60

Query: 61  SALARRAGFDDRLVHGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120
           S  AR AGF+DRLVHG LVASM P +ISSHFPGA+Y+SQS++FKLPVY+GE I   +EAI
Sbjct: 61  SEAARNAGFEDRLVHGTLVASMIPHIISSHFPGAVYLSQSVHFKLPVYIGEEIAAEMEAI 120

Query: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPS 157
           ++R  KKRY+AK + +C +NG+ LVLDGEA AILPS
Sbjct: 121 NIRANKKRYIAKLKIRCFKNGELLVLDGEAMAILPS 155

The following BLAST results are available for this feature:

BLAST of CmaCh20G004700 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PHAJ_AERCA	3.7e-18	39.69	(R)-specific enoyl-CoA hydratase OS=Aeromonas caviae GN=phaJ PE=1 SV=1	[more]
CROR_METEA	2.0e-16	38.14	3-hydroxybutyryl-CoA dehydratase OS=Methylobacterium extorquens (strain ATCC 147...	[more]
PHAJ_RHORT	3.2e-14	33.33	(R)-specific enoyl-CoA hydratase OS=Rhodospirillum rubrum (strain ATCC 11170 / A...	[more]
HTD2_HUMAN	9.4e-14	29.55	Hydroxyacyl-thioester dehydratase type 2, mitochondrial OS=Homo sapiens GN=RPP14...	[more]

BLAST of CmaCh20G004700 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KCN3_CUCSA	6.7e-67	83.33	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G290840 PE=4 SV=1	[more]
A0A067G0J2_CITSI	1.0e-51	70.21	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g031503mg PE=4 SV=1	[more]
W9RN50_9ROSA	1.8e-48	64.10	(R)-specific enoyl-CoA hydratase OS=Morus notabilis GN=L484_014415 PE=4 SV=1	[more]
A0A0B2R270_GLYSO	3.1e-48	66.67	(R)-specific enoyl-CoA hydratase OS=Glycine soja GN=glysoja_038244 PE=4 SV=1	[more]
I1M7A1_SOYBN	3.1e-48	66.67	Uncharacterized protein OS=Glycine max GN=GLYMA_14G041000 PE=4 SV=1	[more]

BLAST of CmaCh20G004700 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G60335.1	6.8e-47	62.94	Thioesterase superfamily protein	[more]

BLAST of CmaCh20G004700 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449463667\|ref\|XP_004149553.1\|	9.6e-67	83.33	PREDICTED: hydroxyacyl-thioester dehydratase type 2, mitochondrial [Cucumis sati...	[more]
gi\|659127833\|ref\|XP_008463911.1\|	2.8e-66	83.33	PREDICTED: hydroxyacyl-thioester dehydratase type 2, mitochondrial [Cucumis melo...	[more]
gi\|568869910\|ref\|XP_006488157.1\|	1.5e-51	70.21	PREDICTED: (R)-specific enoyl-CoA hydratase [Citrus sinensis]	[more]
gi\|828296228\|ref\|XP_004491188.2\|	1.6e-50	68.31	PREDICTED: hydroxyacyl-thioester dehydratase type 2, mitochondrial [Cicer arieti...	[more]
gi\|703128568\|ref\|XP_010104130.1\|	2.6e-48	64.10	(R)-specific enoyl-CoA hydratase [Morus notabilis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002539	MaoC_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0046034	ATP metabolic process
biological_process	GO:0046939	nucleotide phosphorylation
biological_process	GO:0006144	purine nucleobase metabolic process
biological_process	GO:0006633	fatty acid biosynthetic process
biological_process	GO:0009853	photorespiration
biological_process	GO:0010027	thylakoid membrane organization
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0004017	adenylate kinase activity
molecular_function	GO:0019171	3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
molecular_function	GO:0016887	ATPase activity
molecular_function	GO:0005524	ATP binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh20G004700.1	CmaCh20G004700.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002539	MaoC-like domain	PFAM	PF01575	MaoC_dehydratas	coord: 38..128 score: 1.4
None	No IPR available	PANTHER	PTHR13078	FAMILY NOT NAMED	coord: 26..156 score: 1.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh20G004700	Cucsa.052760	Cucumber (Gy14) v1	cgycmaB0059
CmaCh20G004700	Cla000549	Watermelon (97103) v1	cmawmB524
CmaCh20G004700	Csa6G290840	Cucumber (Chinese Long) v2	cmacuB557
CmaCh20G004700	MELO3C025746	Melon (DHL92) v3.5.1	cmameB489
CmaCh20G004700	ClCG02G020940	Watermelon (Charleston Gray)	cmawcgB481
CmaCh20G004700	CSPI06G16250	Wild cucumber (PI 183967)	cmacpiB563
CmaCh20G004700	CmoCh20G005050	Cucurbita moschata (Rifu)	cmacmoB550
CmaCh20G004700	Lsi10G012910	Bottle gourd (USVL1VR-Ls)	cmalsiB506
CmaCh20G004700	Cp4.1LG16g05470	Cucurbita pepo (Zucchini)	cmacpeB564
CmaCh20G004700	MELO3C025746.2	Melon (DHL92) v3.6.1	cmamedB568
CmaCh20G004700	CsaV3_6G021630	Cucumber (Chinese Long) v3	cmacucB0669
CmaCh20G004700	Cla97C02G046970	Watermelon (97103) v2	cmawmbB568
CmaCh20G004700	Bhi10G000476	Wax gourd	cmawgoB0692
CmaCh20G004700	CsGy6G015770	Cucumber (Gy14) v2	cgybcmaB793
CmaCh20G004700	Carg03256	Silver-seed gourd	carcmaB0473
CmaCh20G004700	Carg20235	Silver-seed gourd	carcmaB0424

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh20G004700	Cucumber (Gy14) v2	cgybcmaB388
CmaCh20G004700	Cucumber (Gy14) v2	cgybcmaB790
CmaCh20G004700	Cucumber (Gy14) v2	cgybcmaB801
CmaCh20G004700	Melon (DHL92) v3.6.1	cmamedB597
CmaCh20G004700	Cucumber (Chinese Long) v3	cmacucB0649
CmaCh20G004700	Cucumber (Chinese Long) v3	cmacucB0656
CmaCh20G004700	Watermelon (97103) v2	cmawmbB565
CmaCh20G004700	Watermelon (97103) v2	cmawmbB594
CmaCh20G004700	Wax gourd	cmawgoB0674
CmaCh20G004700	Cucurbita maxima (Rimu)	cmacmaB261
CmaCh20G004700	Cucurbita maxima (Rimu)	cmacmaB468
CmaCh20G004700	Cucurbita maxima (Rimu)	cmacmaB490
CmaCh20G004700	Cucumber (Gy14) v1	cgycmaB0057
CmaCh20G004700	Cucumber (Gy14) v1	cgycmaB0842
CmaCh20G004700	Cucurbita moschata (Rifu)	cmacmoB544
CmaCh20G004700	Cucurbita moschata (Rifu)	cmacmoB553
CmaCh20G004700	Cucurbita moschata (Rifu)	cmacmoB569
CmaCh20G004700	Wild cucumber (PI 183967)	cmacpiB551
CmaCh20G004700	Cucumber (Chinese Long) v2	cmacuB547
CmaCh20G004700	Melon (DHL92) v3.5.1	cmameB520
CmaCh20G004700	Watermelon (Charleston Gray)	cmawcgB476
CmaCh20G004700	Watermelon (97103) v1	cmawmB513
CmaCh20G004700	Cucurbita pepo (Zucchini)	cmacpeB584
CmaCh20G004700	Bottle gourd (USVL1VR-Ls)	cmalsiB518
CmaCh20G004700	Bottle gourd (USVL1VR-Ls)	cmalsiB520
CmaCh20G004700	Bottle gourd (USVL1VR-Ls)	cmalsiB524