CmaCh20G002870 (gene) Cucurbita maxima (Rimu)

NameCmaCh20G002870
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionRibosomal RNA large subunit methyltransferase I
LocationCma_Chr20 : 1391216 .. 1394646 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGTTTGGAATTGGGTGTCGTCCGAATTCCCGCTCAAGAGATAAGAGGAAGGAGTTACCTGGCCTCATCGACAAACGCCGAAGCATGCAGCAGCTTCAAGCCCGCTTGCTAAAGTCTCTTTCAATTTCTTCTTCACCGGCCGCCTCAACAATCCAAGAAATCGCTTCCTCTCGCCCCAAAGGTCAGTCTGTGATTTACATCCCTCGTTTGCGACCTGCAAAACAAATGTCGATTGTCCCAATTCGTTTATGTTTCTTCCCCGCATTTAGCTTCTGCTTCGTCAAAATCCATGTTTATCGATTACGCTTTTAAACTGGTCATATTGATGGGATACTGGTAATGCGATTGTGTAAAGAATTGTGTTGAATTTGGGGGTTTCAGGTTTATCCAAGGTTGTGCTAAAAAAGGGGAAGACACAACTCTTTAAAGATGGGAGTCCGATGGTGTACAGTGGAGCTGTTGATAGAATAATTGGAAGGCCGCCGCCAAAAACTGGAGATGTTGTCCTGGTAGCCGATGGGGCTGAGAAACCTATAGGGTGGGGTTTGTATAACTCAGTTTCCATGTTCTCTGTCAGATTGATGCAGCTGGAAGAGGAGGCAGCTAGGTACTTAATTGGGCAATGCTTGAACTCTGTGACTAACATCACCTTCTTTATGTGGTGCTAAGTTTCTAATTTATGCAATCCAGAGATCCTTCGTGCACATTAGATATGCAGAAACTGATTGAAACCAGGATACATATTGCTAGAGAATTAAGAAAGAGCTTGGGACTTCCGTCGGCAAGTACGAATGCATACCGTCTCGTTAATAGTGAAGGAGATAGGTATCGACCTCGAGTATTCTGTTCATGTCTTCATACATTATGCATTCCCTATCTGTAAAATTTGATTAAATCTAGCACCACCATACATTGATCTTTGTTCTTAAGCATTTTGAAAGTCATTCCATACCGACCCAACTCTTTCTGTTTGCATGTGCGCGCATTCAGAGGGAGGGGTGTGTGAATCTCTTAGACGGTAAATAGATTGTTTGTGTACTGGAACCTATAATAGCCTTATCCAAATGTCCTAGAATTATTGTTTTTTTTTTTCTTTTTGGCAGATTGTCAGGACTAATCATTGACGTCTTTGGAGATTTAGCAGTTGTGGCATCATCGGCTGCTTGGGTGGAGAAGTACAAAGCTGAAATCGAGGACTGTGTTCGTGGAATTGATGAAATCAATCATGTCAAATGGAGACCATCGGTGGAAATTCTGAAGGAAGAAGGGATAGATGTGTCTGAATTGAAGGATACAAATCCGTCTAATTGTCCTGAAAGAACAAAGGTAACAAGCGACTGCAACACTTGTACTGTTTCAAGAAATGCTCTATATCATGTTCATGGTAACTAAATGTTAAAAATGTGGAGCCCTTTTGATGAGTAAACTGATCTTACATGCTTGAATAATCATCAATGAGTATCAAAGATTTGGAAACTAGGAGATAAGAGTTGCTGATATAAGTAAGCTTTATGCACAAATCCTCACTGGGATGTGTTCTAGCAGGTGGAAATTCTTGGTAACAAGAGAAGCTATGAGGGTATCATGTTAATGTAGAAAGTAGTTCAAAAGCTATATAATACATACAACCAAGTTATTACCACAAAATCAGATGAGATATTGAACATGCCTTATTCTGATAGGTAACGGAGAATGGAATTTTCTATGCCATTTCACTTGAAGGCCAGAAAACAGGGTTTTATGCTGATCAGCGTGAAAATCGTCATTTTATCTCAACAATTTCTGAGGGTCAAAAAGTTCTTGACCTCTGCTGCTACAGTGGTGGGTTTTCTCTTAATGCAGCACGTGGTGGAGCTATTAATGTCACCGGTATACATTTTTTCTTGTCTTCTATTAAAGCTTTTAGATCAAATGTTTAGGAGAGTATGCTGTGGCAAAGTCACTAATTATTCATACTTAGAAAAAAATTCAATCTTTTGTGTGTGTTTATCTGTTGCTATCACGGAAAAGAAACAGGATTCTCTAGCCTTGAATACAGTGGTACTATTCTCATAAGTTCGCTAATTATCAGGTATTGATTCATCACTGCCTGCTCTGGAGCTAGCAAAAGAAAATGTAATCCTTAATAGTATGGATCCTGACAGAATATCATTTTTGAAAGCTGATGCCACTACATTCATGAAGGATGCTCTATCTAGGAATGAATGTTGGGATATAGTCATTTTGGATCCTCCTAAACTAGCTCCACGAAAAAAGGTATGATGCATTTGATTATTTTTCCTGTTACGGAAGTTCTTTGATTAATTAATTCTATGATCTTACAAGTTAGGCCCCATGTGAAATACTGGTACAAAGTGAACATAAGTTCTTAATTCTTTGATTCTGTTCACTCTTACAAGTTAGAATCCACGTGAAATCCTATTAACTATTTGGTGTGGGTGGTTTTTAAACGTCAGAAAGTTGTGGATTTTAAGATGATAGATCAAATTGTTCAATACATCATCCGGGGCTTGTTTTGAAAGTATCAAGTTTGTTAACATTCATTGTCTGCTTGTCTAAACGACCCAAGTCTATTATCTATTCTACCTGTGCTTATTAAAAATTTGTTTGCTTCATCACCCTACTAACTAAAACTTAGTAGGTTCTACAAACTGCATCAGGCATGTACAGGACTTTAAATTCATTAGCAATGCAACTCACAAGGAAAGGTGGTCTTCTCATGACTTGTTCATGTTCTGGAGCAATGACTCAGAGTGGGATGTTCCTGCAAACACTCCAGGCAAGTCCAGTTCCGCCCCCACCAACCATGAAAACAAATCATCACTATTACTTATTTCAACGCCTTAGAAACATTGTTTGGTAATTATATAGTTTCTATACCTCATGATCTTGACTTAAACTATATGAAGAGTTATTTAGGATAATATCTACGTGTCATGTACGAAAAGAACCTATTCGGCTTGTTTAAGAAAACTAAACTGGTTTGTACTGCCCCTTTCATTTAAACAGGGTGCAGCATCAATGGCCAAAAAGCAAATCACGGTCCTACGACAAGCAGGAGCAGCATGTGATCACCCCATTGACCCTACTTACCCAGAGGGTGCATATCTTTCTAATATCCTTCTACGTGTGCTCTAAACTTTATGAAGTTATAAATGTATCTACCATTGATCAAAAGAGACAAGCCATTTAACATAAGGAGATGAGATACTGCATTTGAAGTTACACTATCGTCAGAGCCACATCTTCATATAAGAAGGAAAGTAGAAGTTCCATTACAACAATCTGTCATACACCCTGCCCTGCCCTTGTTTCTTATATGCTCACATCAAAATAAAATTACAAATAAAATTACAACAAACCGTGTTCTAGCAGCTCCTGCTTCAGTGCCCGCTCGGGTTTCTATTCCACGACAGATATGATGGGTCCCC

mRNA sequence

GGGTTTGGAATTGGGTGTCGTCCGAATTCCCGCTCAAGAGATAAGAGGAAGGAGTTACCTGGCCTCATCGACAAACGCCGAAGCATGCAGCAGCTTCAAGCCCGCTTGCTAAAGTCTCTTTCAATTTCTTCTTCACCGGCCGCCTCAACAATCCAAGAAATCGCTTCCTCTCGCCCCAAAGGTTTATCCAAGGTTGTGCTAAAAAAGGGGAAGACACAACTCTTTAAAGATGGGAGTCCGATGGTGTACAGTGGAGCTGTTGATAGAATAATTGGAAGGCCGCCGCCAAAAACTGGAGATGTTGTCCTGGTAGCCGATGGGGCTGAGAAACCTATAGGGTGGGGTTTGTATAACTCAGTTTCCATGTTCTCTGTCAGATTGATGCAGCTGGAAGAGGAGGCAGCTAGAGATCCTTCGTGCACATTAGATATGCAGAAACTGATTGAAACCAGGATACATATTGCTAGAGAATTAAGAAAGAGCTTGGGACTTCCGTCGGCAAGTACGAATGCATACCGTCTCGTTAATAGTGAAGGAGATAGATTGTCAGGACTAATCATTGACGTCTTTGGAGATTTAGCAGTTGTGGCATCATCGGCTGCTTGGGTGGAGAAGTACAAAGCTGAAATCGAGGACTGTGTTCGTGGAATTGATGAAATCAATCATGTCAAATGGAGACCATCGGTGGAAATTCTGAAGGAAGAAGGGATAGATGTGTCTGAATTGAAGGATACAAATCCGTCTAATTGTCCTGAAAGAACAAAGGTAACGGAGAATGGAATTTTCTATGCCATTTCACTTGAAGGCCAGAAAACAGGGTTTTATGCTGATCAGCGTGAAAATCGTCATTTTATCTCAACAATTTCTGAGGGTCAAAAAGTTCTTGACCTCTGCTGCTACAGTGGTGGGTTTTCTCTTAATGCAGCACGTGGTGGAGCTATTAATGTCACCGGTATTGATTCATCACTGCCTGCTCTGGAGCTAGCAAAAGAAAATGTAATCCTTAATAGTATGGATCCTGACAGAATATCATTTTTGAAAGCTGATGCCACTACATTCATGAAGGATGCTCTATCTAGGAATGAATGTTGGGATATAGTCATTTTGGATCCTCCTAAACTAGCTCCACGAAAAAAGGGTGCAGCATCAATGGCCAAAAAGCAAATCACGGTCCTACGACAAGCAGGAGCAGCATGTGATCACCCCATTGACCCTACTTACCCAGAGGGTGCATATCTTTCTAATATCCTTCTACGTGTGCTCTAAACTTTATGAAGTTATAAATGTATCTACCATTGATCAAAAGAGACAAGCCATTTAACATAAGGAGATGAGATACTGCATTTGAAGTTACACTATCGTCAGAGCCACATCTTCATATAAGAAGGAAAGTAGAAGTTCCATTACAACAATCTGTCATACACCCTGCCCTGCCCTTGTTTCTTATATGCTCACATCAAAATAAAATTACAAATAAAATTACAACAAACCGTGTTCTAGCAGCTCCTGCTTCAGTGCCCGCTCGGGTTTCTATTCCACGACAGATATGATGGGTCCCC

Coding sequence (CDS)

ATGCAGCAGCTTCAAGCCCGCTTGCTAAAGTCTCTTTCAATTTCTTCTTCACCGGCCGCCTCAACAATCCAAGAAATCGCTTCCTCTCGCCCCAAAGGTTTATCCAAGGTTGTGCTAAAAAAGGGGAAGACACAACTCTTTAAAGATGGGAGTCCGATGGTGTACAGTGGAGCTGTTGATAGAATAATTGGAAGGCCGCCGCCAAAAACTGGAGATGTTGTCCTGGTAGCCGATGGGGCTGAGAAACCTATAGGGTGGGGTTTGTATAACTCAGTTTCCATGTTCTCTGTCAGATTGATGCAGCTGGAAGAGGAGGCAGCTAGAGATCCTTCGTGCACATTAGATATGCAGAAACTGATTGAAACCAGGATACATATTGCTAGAGAATTAAGAAAGAGCTTGGGACTTCCGTCGGCAAGTACGAATGCATACCGTCTCGTTAATAGTGAAGGAGATAGATTGTCAGGACTAATCATTGACGTCTTTGGAGATTTAGCAGTTGTGGCATCATCGGCTGCTTGGGTGGAGAAGTACAAAGCTGAAATCGAGGACTGTGTTCGTGGAATTGATGAAATCAATCATGTCAAATGGAGACCATCGGTGGAAATTCTGAAGGAAGAAGGGATAGATGTGTCTGAATTGAAGGATACAAATCCGTCTAATTGTCCTGAAAGAACAAAGGTAACGGAGAATGGAATTTTCTATGCCATTTCACTTGAAGGCCAGAAAACAGGGTTTTATGCTGATCAGCGTGAAAATCGTCATTTTATCTCAACAATTTCTGAGGGTCAAAAAGTTCTTGACCTCTGCTGCTACAGTGGTGGGTTTTCTCTTAATGCAGCACGTGGTGGAGCTATTAATGTCACCGGTATTGATTCATCACTGCCTGCTCTGGAGCTAGCAAAAGAAAATGTAATCCTTAATAGTATGGATCCTGACAGAATATCATTTTTGAAAGCTGATGCCACTACATTCATGAAGGATGCTCTATCTAGGAATGAATGTTGGGATATAGTCATTTTGGATCCTCCTAAACTAGCTCCACGAAAAAAGGGTGCAGCATCAATGGCCAAAAAGCAAATCACGGTCCTACGACAAGCAGGAGCAGCATGTGATCACCCCATTGACCCTACTTACCCAGAGGGTGCATATCTTTCTAATATCCTTCTACGTGTGCTCTAA

Protein sequence

MQQLQARLLKSLSISSSPAASTIQEIASSRPKGLSKVVLKKGKTQLFKDGSPMVYSGAVDRIIGRPPPKTGDVVLVADGAEKPIGWGLYNSVSMFSVRLMQLEEEAARDPSCTLDMQKLIETRIHIARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFYAISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSLPALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKGAASMAKKQITVLRQAGAACDHPIDPTYPEGAYLSNILLRVL
BLAST of CmaCh20G002870 vs. Swiss-Prot
Match: RLMI_ALTMD (Ribosomal RNA large subunit methyltransferase I OS=Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype) GN=rlmI PE=3 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 4.5e-38
Identity = 108/318 (33.96%), Postives = 170/318 (53.46%), Query Frame = 1

Query: 35  SKVVLKKGKTQLFKDGSPMVYSGAVDRIIGRPPPKTGDVVLVADGAEKPIGWGLYNSVSM 94
           ++V+L+  + +  +   P V+  AV  + GR   + GD V V D     +G G Y+  S 
Sbjct: 3   AQVILQPSRDKSLRRKHPWVFESAVAELKGRA--RIGDTVDVFDDEGDWLGRGAYSPHSK 62

Query: 95  FSVRLMQLEEEAARDPSCTLDMQKLIETRIHIARELRKSLGLPSASTNAYRLVNSEGDRL 154
             VR+   +++ + D    L        R+  A  LRK L  P+  TNA+R + SE D L
Sbjct: 63  IRVRMWTFKKDESIDNGFFL-------RRLETALALRKRLFDPN-KTNAFRWIASESDGL 122

Query: 155 SGLIIDVFGDLAVVASSAAWVEKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGID-VSE 214
            G+ ID++ ++AVV   +A  EK++ +I   +  +    HV  R  V++ K+EG++ V+ 
Sbjct: 123 PGVTIDLYDNVAVVQLLSAGGEKHRDKIVWAITKLMPDVHVYERSDVDVRKKEGLEPVTG 182

Query: 215 LKDTNPSNCPERTKVTENGIFYAISLE-GQKTGFYADQRENRHFISTISEGQKVLDLCCY 274
           +    P   P +  V ENGI   + +E G KTGFY DQR++R   +  ++   VL+   Y
Sbjct: 183 VLHGQP---PTQVTVKENGINIVVDIESGHKTGFYLDQRDSRAAAAHYAKDADVLNCFSY 242

Query: 275 SGGFSLNAARGGAINVTGIDSSLPALELAKENVILNSMDPDRISFLKADATTFMKDALSR 334
           +G FS  A  GGA +VT +D S PAL+LAK +V +N  D  R  +L  D    +++   +
Sbjct: 243 TGTFSCYALSGGAKSVTNVDVSQPALDLAKHHVAINGFDDKRTQYLNKDVFKALREYHEQ 302

Query: 335 NECWDIVILDPPKLAPRK 351
           N+ +D+VILDPPK    K
Sbjct: 303 NKQFDMVILDPPKFVDNK 307

BLAST of CmaCh20G002870 vs. Swiss-Prot
Match: RLMI_PSEHT (Ribosomal RNA large subunit methyltransferase I OS=Pseudoalteromonas haloplanktis (strain TAC 125) GN=rlmI PE=3 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.0e-37
Identity = 117/405 (28.89%), Postives = 182/405 (44.94%), Query Frame = 1

Query: 35  SKVVLKKGKTQLFKDGSPMVYSGAVDRIIGRPPPKTGDVVLVADGAEKPIGWGLYNSVSM 94
           S V L+ G+ +  K   P ++S A+ +I G+P    GD V + D   K +    Y+  S 
Sbjct: 3   SAVYLQAGRDKSLKRKHPWLFSKAIKKIKGKPG--LGDTVTIHDSEGKFLATAAYSPHSQ 62

Query: 95  FSVRLMQLEEEAARDPSCTLDMQKLIETRIHIARELRKSLGLPSASTNAYRLVNSEGDRL 154
              R+   +E+   D       Q   E R+  A E R  + +       +RL  +E D L
Sbjct: 63  IRARVWSFDEKEVID-------QHFFERRLRRALEARSQV-IEEGGLTGFRLCAAESDFL 122

Query: 155 SGLIIDVFGDLAVVASSAAWVEKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSEL 214
            G+ ID F ++ V    +A  E++K EI   +  I     +  R  VE+  +EG++   +
Sbjct: 123 PGVTIDKFDNVLVCQLLSAGAERHKGEIVGALMAIFPGMSIYERSDVEVRTKEGLE--PI 182

Query: 215 KDTNPSNCPER-TKVTENGIFYAIS-LEGQKTGFYADQRENRHFISTISEGQKVLDLCCY 274
           K     N P     + ENG    +  ++G KTGFY DQR++R  +   S+ + VL+   Y
Sbjct: 183 KGALWGNEPTAPVLIAENGFKIEVDIIDGHKTGFYLDQRDSRAALERFSKDKTVLNCFSY 242

Query: 275 SGGFSLNAARGGAINVTGIDSSLPALELAKENVILNSMDPDRISFLKADATTFMKDALSR 334
           +G FSL A RGG  +VT +D S PAL+ AK NV  N++D D++ F+K D    ++     
Sbjct: 243 TGTFSLYALRGGCKHVTNVDVSQPALDTAKRNVEHNNLDLDKVDFVKQDVFKLLRQYRED 302

Query: 335 NECWDIVILDPPKLAPRK---KGAA------SMAKKQIT--------------------- 393
              +D +++DPPK A  K    GA       +M   QI                      
Sbjct: 303 GVLFDTIVMDPPKFADNKAQLTGACRGYKDINMIAMQILKPGGTLLTFSCSGLMEQNLFQ 362

BLAST of CmaCh20G002870 vs. Swiss-Prot
Match: RLMI_ECOSM (Ribosomal RNA large subunit methyltransferase I OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=rlmI PE=3 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 2.5e-36
Identity = 108/404 (26.73%), Postives = 186/404 (46.04%), Query Frame = 1

Query: 36  KVVLKKGKTQLFKDGSPMVYSGAVDRIIGRPPPKTGDVVLVADGAEKPIGWGLYNSVSMF 95
           ++VL KG+ +      P ++SGAV R+ G+     G+ + + D   K +  G Y+  S  
Sbjct: 4   RLVLAKGREKSLLRRHPWIFSGAVARMEGKA--SLGETIDIVDHQGKWLARGAYSPASQI 63

Query: 96  SVRLMQLEEEAARDPSCTLDMQKLIETRIHIARELRKSLGLPSASTNAYRLVNSEGDRLS 155
             R+         DPS ++D+      R+  A++ R  L       N+YRL+  E D L 
Sbjct: 64  RARVWTF------DPSESIDIA-FFTRRLQQAQKWRDWLAQKDG-LNSYRLIAGESDGLP 123

Query: 156 GLIIDVFGDLAVVASSAAWVEKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELK 215
           G+ ID FG+  V+   +A  E  +A +   ++ +     +  R  V + K+EG+++++  
Sbjct: 124 GITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVAVRKKEGMELTQGP 183

Query: 216 DTNPSNCPERTKVTENGIFYAISLE-GQKTGFYADQRENRHFISTISEGQKVLDLCCYSG 275
            T     P    + E+G+   + ++ G KTG+Y DQR++R       E ++VL+   Y+G
Sbjct: 184 VTGELP-PALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRYVENKRVLNCFSYTG 243

Query: 276 GFSLNAARGGAINVTGIDSSLPALELAKENVILNSMDPDRISFLKADATTFMKDALSRNE 335
           GF+++A  GG   V  +D+S  AL++A++NV LN +D  +  F++ D    ++    R E
Sbjct: 244 GFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFVRDDVFKLLRTYRDRGE 303

Query: 336 CWDIVILDPPKLAPRK-------KGAASMAKKQITVLRQAGA------------------ 394
            +D++++DPPK    K       +G   +    I +L + G                   
Sbjct: 304 KFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGVLLTFSCSGLMTSDLFQKI 363

BLAST of CmaCh20G002870 vs. Swiss-Prot
Match: RLMI_ECOLC (Ribosomal RNA large subunit methyltransferase I OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) GN=rlmI PE=3 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 5.5e-36
Identity = 108/404 (26.73%), Postives = 186/404 (46.04%), Query Frame = 1

Query: 36  KVVLKKGKTQLFKDGSPMVYSGAVDRIIGRPPPKTGDVVLVADGAEKPIGWGLYNSVSMF 95
           ++VL KG+ +      P V+SGAV R+ G+     G+ + + D   K +  G Y+  S  
Sbjct: 4   RLVLAKGREKSLLRRHPWVFSGAVARMEGKA--SLGETIDIVDHQGKWLARGAYSPASQI 63

Query: 96  SVRLMQLEEEAARDPSCTLDMQKLIETRIHIARELRKSLGLPSASTNAYRLVNSEGDRLS 155
             R+         DPS ++D+      R+  A++ R  L       ++YRL+  E D L 
Sbjct: 64  RARVWTF------DPSESIDIA-FFSRRLQQAQKWRDWLAQKDG-LDSYRLIAGESDGLP 123

Query: 156 GLIIDVFGDLAVVASSAAWVEKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELK 215
           G+ ID FG+  V+   +A  E  +A +   ++ +     +  R  V + K+EG+++++  
Sbjct: 124 GITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECSIYDRSDVAVRKKEGMELTQGP 183

Query: 216 DTNPSNCPERTKVTENGIFYAISLE-GQKTGFYADQRENRHFISTISEGQKVLDLCCYSG 275
            T     P    + E+G+   + ++ G KTG+Y DQR++R       E ++VL+   Y+G
Sbjct: 184 VTGELP-PALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRYVENKRVLNCFSYTG 243

Query: 276 GFSLNAARGGAINVTGIDSSLPALELAKENVILNSMDPDRISFLKADATTFMKDALSRNE 335
           GF+++A  GG   V  +D+S  AL++A++NV LN +D  +  F++ D    ++    R E
Sbjct: 244 GFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFVRDDVFKLLRTYRDRGE 303

Query: 336 CWDIVILDPPKLAPRK-------KGAASMAKKQITVLRQAG------------------- 394
            +D++++DPPK    K       +G   +    I +L + G                   
Sbjct: 304 KFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGILLTFSCSGLMTSDLFQKI 363

BLAST of CmaCh20G002870 vs. Swiss-Prot
Match: RLMI_ECOLI (Ribosomal RNA large subunit methyltransferase I OS=Escherichia coli (strain K12) GN=rlmI PE=1 SV=2)

HSP 1 Score: 153.3 bits (386), Expect = 5.5e-36
Identity = 108/404 (26.73%), Postives = 186/404 (46.04%), Query Frame = 1

Query: 36  KVVLKKGKTQLFKDGSPMVYSGAVDRIIGRPPPKTGDVVLVADGAEKPIGWGLYNSVSMF 95
           ++VL KG+ +      P V+SGAV R+ G+     G+ + + D   K +  G Y+  S  
Sbjct: 4   RLVLAKGREKSLLRRHPWVFSGAVARMEGKA--SLGETIDIVDHQGKWLARGAYSPASQI 63

Query: 96  SVRLMQLEEEAARDPSCTLDMQKLIETRIHIARELRKSLGLPSASTNAYRLVNSEGDRLS 155
             R+         DPS ++D+      R+  A++ R  L       ++YRL+  E D L 
Sbjct: 64  RARVWTF------DPSESIDIA-FFSRRLQQAQKWRDWLAQKDG-LDSYRLIAGESDGLP 123

Query: 156 GLIIDVFGDLAVVASSAAWVEKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELK 215
           G+ ID FG+  V+   +A  E  +A +   ++ +     +  R  V + K+EG+++++  
Sbjct: 124 GITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECSIYDRSDVAVRKKEGMELTQGP 183

Query: 216 DTNPSNCPERTKVTENGIFYAISLE-GQKTGFYADQRENRHFISTISEGQKVLDLCCYSG 275
            T     P    + E+G+   + ++ G KTG+Y DQR++R       E ++VL+   Y+G
Sbjct: 184 VTGELP-PALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRYVENKRVLNCFSYTG 243

Query: 276 GFSLNAARGGAINVTGIDSSLPALELAKENVILNSMDPDRISFLKADATTFMKDALSRNE 335
           GF+++A  GG   V  +D+S  AL++A++NV LN +D  +  F++ D    ++    R E
Sbjct: 244 GFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFVRDDVFKLLRTYRDRGE 303

Query: 336 CWDIVILDPPKLAPRK-------KGAASMAKKQITVLRQAG------------------- 394
            +D++++DPPK    K       +G   +    I +L + G                   
Sbjct: 304 KFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGILLTFSCSGLMTSDLFQKI 363

BLAST of CmaCh20G002870 vs. TrEMBL
Match: A0A0A0KB85_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G135980 PE=4 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 1.8e-171
Identity = 314/387 (81.14%), Postives = 329/387 (85.01%), Query Frame = 1

Query: 53  MVYSGAVDRIIGRPPPKTGDVVLVADGAEKPIGWGLYNSVSMFSVRLMQLEEEAARDPSC 112
           MVYSGAVDRIIGRPPPKTGDVVLVADG EKPIGWGLYNSVSMFSVRLMQLEEEAARDPSC
Sbjct: 1   MVYSGAVDRIIGRPPPKTGDVVLVADGTEKPIGWGLYNSVSMFSVRLMQLEEEAARDPSC 60

Query: 113 TLDMQKLIETRIHIARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSA 172
           TL+++K+IETR+H ARELRK+LGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSA
Sbjct: 61  TLNVEKMIETRVHTARELRKNLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSA 120

Query: 173 AWVEKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENG 232
           AWVEKYKAEIEDCVRGI+EINHVKWRPSV+ILKEEGIDVSELKDT+PS CPERTKV EN 
Sbjct: 121 AWVEKYKAEIEDCVRGIEEINHVKWRPSVDILKEEGIDVSELKDTSPSTCPERTKVMEND 180

Query: 233 IFYAISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGID 292
           IFYAISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNA RGGAINVTGID
Sbjct: 181 IFYAISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNALRGGAINVTGID 240

Query: 293 SSLPALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKK- 352
           SSLPALELAKENV+LNSMDP+RISFLKADAT FMKDALSRNE WD+VILDPPKLAPRKK 
Sbjct: 241 SSLPALELAKENVVLNSMDPERISFLKADATAFMKDALSRNESWDVVILDPPKLAPRKKV 300

Query: 353 --GAASMAKK------QIT-------------------------------------VLRQ 394
              A+ M +       Q+T                                     VLRQ
Sbjct: 301 LQTASGMYRNLNSLAMQLTRKGGFLMTCSCSGAMTQSGMFLQILQGAASTAKKKITVLRQ 360

BLAST of CmaCh20G002870 vs. TrEMBL
Match: M5WGM9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005882mg PE=4 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 5.0e-169
Identity = 307/439 (69.93%), Postives = 355/439 (80.87%), Query Frame = 1

Query: 1   MQQLQARLLKSLSISSSPAASTIQEIASSRPKGLSKVVLKKGKTQLFKDGSPMVYSGAVD 60
           MQ L ARL+KSLS +S P  +T+QE+AS+ PKG+++VVLKKGKTQLFKDGSPMVYSGAVD
Sbjct: 1   MQHLPARLVKSLSATSLPCTTTLQELASAHPKGVARVVLKKGKTQLFKDGSPMVYSGAVD 60

Query: 61  RIIGRPPPKTGDVVLVADGAEKPIGWGLYNSVSMFSVRLMQLEEEAARDPSCTLDMQKLI 120
           RIIGRPPP++GD+VLVADGAEKPIGWG+YNSVSMF VRLMQLEEEA RD SC L+M+KL+
Sbjct: 61  RIIGRPPPRSGDIVLVADGAEKPIGWGMYNSVSMFCVRLMQLEEEATRDLSCALNMEKLL 120

Query: 121 ETRIHIARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKA 180
           ETRI+ A ELRKSLGLPSASTNA+RLVNSEGDRLSGLI+DVFGD+AV+ASSAAWVEKYK 
Sbjct: 121 ETRINEAIELRKSLGLPSASTNAFRLVNSEGDRLSGLIVDVFGDVAVIASSAAWVEKYKP 180

Query: 181 EIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFYAISLE 240
           E+E  V  IDEIN++ WRPS E+LKEEG+D S  K+ +PS CP+RTKVTENGIFYAISLE
Sbjct: 181 EVEASVGRIDEINYINWRPSSEVLKEEGLDASNYKERDPSICPQRTKVTENGIFYAISLE 240

Query: 241 GQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSLPALEL 300
           GQKTGFYADQRENR FISTIS+GQ+VLD+ CYSGGF+LNAARGGA+NV G+DSSLPA+EL
Sbjct: 241 GQKTGFYADQRENRQFISTISDGQRVLDMFCYSGGFALNAARGGAVNVIGVDSSLPAVEL 300

Query: 301 AKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKK---GAASMA 360
           AKEN++LN+MDP RI+FL+ DA+ FMK ALSRNE WDIVILDPPKLAPRKK    A+ M 
Sbjct: 301 AKENIVLNNMDPGRITFLREDASEFMKGALSRNESWDIVILDPPKLAPRKKALQNASGMY 360

Query: 361 KK------QIT-------------------------------------VLRQAGAACDHP 394
           +       Q+T                                     +LR+AGAACDHP
Sbjct: 361 RNLNSLAMQLTKRGGLLMTCSCSGAMTQSGMFLRILQGAASAAGRKITILREAGAACDHP 420

BLAST of CmaCh20G002870 vs. TrEMBL
Match: A0A0D2PUQ0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_001G051600 PE=4 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 1.9e-168
Identity = 305/447 (68.23%), Postives = 356/447 (79.64%), Query Frame = 1

Query: 2   QQLQARLLKSLSISSS---------PAASTIQEIASSRPKGLSKVVLKKGKTQLFKDGSP 61
           QQLQARL+K LS S S         P  S++Q  ASS+PKG++KV+LKKGKTQLFKDGSP
Sbjct: 4   QQLQARLMKCLSCSHSHLPLPPPPPPPFSSLQRFASSQPKGVAKVILKKGKTQLFKDGSP 63

Query: 62  MVYSGAVDRIIGRPPPKTGDVVLVADGAEKPIGWGLYNSVSMFSVRLMQLEEEAARDPSC 121
           MVYSGA+DRIIGRPPPKTGD+VLVADGAEKPIGWGLYNSVSMF VRLMQLEEEA RDPSC
Sbjct: 64  MVYSGAIDRIIGRPPPKTGDIVLVADGAEKPIGWGLYNSVSMFCVRLMQLEEEATRDPSC 123

Query: 122 TLDMQKLIETRIHIARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSA 181
            LDM+KL+ETRI+ A ELR+ LGLPSA+TNAYRLVNSEGDRLSGLI+DVFGDLAVVASSA
Sbjct: 124 ALDMEKLLETRINAAVELRRGLGLPSATTNAYRLVNSEGDRLSGLIVDVFGDLAVVASSA 183

Query: 182 AWVEKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENG 241
           AWVEKYK++++ C+  IDEINH+ WRPSVEILKEEG+D ++LK+ +PS CP+RTKV ENG
Sbjct: 184 AWVEKYKSKVKACISSIDEINHIHWRPSVEILKEEGMDAADLKELHPSTCPQRTKVIENG 243

Query: 242 IFYAISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGID 301
           I YAISLEGQKTGFYADQRENR F+STIS GQ+VLD+CCYSGGF+LNAA+GGA+++TG+D
Sbjct: 244 ISYAISLEGQKTGFYADQRENRMFLSTISHGQRVLDVCCYSGGFALNAAKGGAMSITGVD 303

Query: 302 SSLPALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKK- 361
           +SLPALELA+EN+ LN++DP++ SFL+ DAT FMK ALSRN+ WDIVILDPPKLAP +K 
Sbjct: 304 TSLPALELARENIALNNLDPEKTSFLREDATVFMKGALSRNDSWDIVILDPPKLAPSRKV 363

Query: 362 --GAASMAKK------QIT-------------------------------------VLRQ 394
              A+ M +       +IT                                     VLRQ
Sbjct: 364 LQSASGMYRNLNSLAMKITRRGGLLMTCSCSGAMTQSGAFLRTLQGAASMAERKITVLRQ 423

BLAST of CmaCh20G002870 vs. TrEMBL
Match: B9HYB4_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s18100g PE=4 SV=2)

HSP 1 Score: 597.0 bits (1538), Expect = 1.6e-167
Identity = 309/443 (69.75%), Postives = 351/443 (79.23%), Query Frame = 1

Query: 1   MQQLQ--ARLLKSLSISSSPA--ASTIQEIASSRPKGLSKVVLKKGKTQLFKDGSPMVYS 60
           MQQL+  ARL+KSL ISS P+  +ST+Q+IASS  KG++KVVLKKGKTQLFKDGSPMVYS
Sbjct: 1   MQQLKLSARLIKSLPISSLPSTPSSTLQQIASSHSKGIAKVVLKKGKTQLFKDGSPMVYS 60

Query: 61  GAVDRIIGRPPPKTGDVVLVADGAEKPIGWGLYNSVSMFSVRLMQLEEEAARDPSCTLDM 120
           GAVDRIIGRPPPKTGD+VLVADG EKPIGWGLYNSVSMF VRLMQLEEEA RDPSC LDM
Sbjct: 61  GAVDRIIGRPPPKTGDIVLVADGTEKPIGWGLYNSVSMFCVRLMQLEEEATRDPSCALDM 120

Query: 121 QKLIETRIHIARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVE 180
           +KL+ETR + A ELR+ LGLPS+ TNAYRLVNSEGDRLSGLI+DVFGDLAV+ASSAAWVE
Sbjct: 121 KKLLETRTNAAIELRRRLGLPSSHTNAYRLVNSEGDRLSGLIVDVFGDLAVIASSAAWVE 180

Query: 181 KYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFYA 240
            YK E+E C+  ID INH+ WRPSV+ILKEEG+DVS++K+ +PS CPER KV ENGI YA
Sbjct: 181 NYKPEVEACINRIDGINHINWRPSVDILKEEGMDVSDMKEVHPSTCPERIKVMENGISYA 240

Query: 241 ISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSLP 300
           ISL GQKTGFYADQRENR FISTIS GQKVLD+CCYSGGF+LNAA GGAI+VTG+D+S+P
Sbjct: 241 ISLVGQKTGFYADQRENRQFISTISNGQKVLDICCYSGGFALNAAHGGAIDVTGVDTSMP 300

Query: 301 ALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKK---GA 360
           ALELA+EN++LN++DP RISFL+ DA  FMK ALSRNE WDIVILDPPKLAPRKK    A
Sbjct: 301 ALELARENIVLNNLDPGRISFLREDAIQFMKGALSRNESWDIVILDPPKLAPRKKVLQNA 360

Query: 361 ASMAKKQIT-------------------------------------------VLRQAGAA 394
           + M +   +                                           VLR+AGAA
Sbjct: 361 SGMYRNMNSMALRLTKRGGLLMTCSCSGAMTQSGMFLRVLQGAASMAGRKISVLREAGAA 420

BLAST of CmaCh20G002870 vs. TrEMBL
Match: A0A0B0NK02_GOSAR (Ribosomal RNA large subunit methyltransferase I OS=Gossypium arboreum GN=F383_01768 PE=4 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 9.8e-165
Identity = 299/447 (66.89%), Postives = 351/447 (78.52%), Query Frame = 1

Query: 2   QQLQARLLKSLSISSS---------PAASTIQEIASSRPKGLSKVVLKKGKTQLFKDGSP 61
           QQL ARL+K LS S S         P   ++Q  ASS+PKG++KV+LKKGKTQLFKDGSP
Sbjct: 4   QQLHARLMKCLSCSHSHLPLPPPRPPPFYSLQRFASSQPKGVAKVILKKGKTQLFKDGSP 63

Query: 62  MVYSGAVDRIIGRPPPKTGDVVLVADGAEKPIGWGLYNSVSMFSVRLMQLEEEAARDPSC 121
           MVYSGA+DRIIGRPPPKTGD+VLVADG EKPIGWGLYNSVSMF VRLMQLEEEA RDP C
Sbjct: 64  MVYSGAIDRIIGRPPPKTGDIVLVADGTEKPIGWGLYNSVSMFCVRLMQLEEEATRDPFC 123

Query: 122 TLDMQKLIETRIHIARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSA 181
            LDM+KL+ETRI+ A ELR+ LGLPSA+T+AYRLVNSEGDRLSGLI+DVFGDLAVVASSA
Sbjct: 124 ALDMEKLLETRINAAVELRRGLGLPSATTDAYRLVNSEGDRLSGLIVDVFGDLAVVASSA 183

Query: 182 AWVEKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENG 241
           AWVE YK++++ C+  IDEINH+ WRPSVEILKEEG+D ++LK+ +PS CP+RTKV ENG
Sbjct: 184 AWVETYKSKVKACISSIDEINHIHWRPSVEILKEEGMDAADLKELHPSTCPQRTKVIENG 243

Query: 242 IFYAISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGID 301
           I YAISLEGQKTGFYADQRENR F+STIS GQ+VLD+CCYSGGF+LNAA+GGA+++TG+D
Sbjct: 244 ISYAISLEGQKTGFYADQRENRMFLSTISHGQRVLDVCCYSGGFALNAAKGGAMSITGVD 303

Query: 302 SSLPALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKK- 361
           +SLPALELA+EN+ LN++DP++  FL+ DAT FMK ALSRN+ WDIVILDPPKLAPR+K 
Sbjct: 304 TSLPALELARENIALNNLDPEKTFFLREDATVFMKGALSRNDSWDIVILDPPKLAPRRKV 363

Query: 362 --GAASMAKK------QIT-------------------------------------VLRQ 394
              A+ M +       +IT                                     VLRQ
Sbjct: 364 LQSASGMYRNLNSLAMKITRRGGLLMSCSCSGAMTQSGTFLRTLQGAASMAERKITVLRQ 423

BLAST of CmaCh20G002870 vs. TAIR10
Match: AT1G54310.2 (AT1G54310.2 S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 557.8 bits (1436), Expect = 5.5e-159
Identity = 286/437 (65.45%), Postives = 335/437 (76.66%), Query Frame = 1

Query: 3   QLQARLLKSLSISSSPAASTIQEIASSRPKGLSKVVLKKGKTQLFKDGSPMVYSGAVDRI 62
           QL  RL  SL  SSS       +IASSRP+G++KVVLKKGKTQLFKDGSPMVYSGAVDRI
Sbjct: 25  QLPCRLHHSLFYSSS-------QIASSRPQGVAKVVLKKGKTQLFKDGSPMVYSGAVDRI 84

Query: 63  IGRPPPKTGDVVLVADGAEKPIGWGLYNSVSMFSVRLMQLEEEAARDPSCTLDMQKLIET 122
           IG+PPP+TGDVV+VADG E PIGWGLYNSVSMF VRLMQL+ E+ RDPSC L+++KL++T
Sbjct: 85  IGKPPPQTGDVVIVADGTENPIGWGLYNSVSMFCVRLMQLQHESTRDPSCALNIEKLLQT 144

Query: 123 RIHIARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKAEI 182
           RI  A +LRKSL LPSA+TNAYRLVNSEGDRLSGLI+DVFGD+AVVASSAAW+EKY+ E+
Sbjct: 145 RIAEAVQLRKSLALPSANTNAYRLVNSEGDRLSGLIVDVFGDIAVVASSAAWLEKYRIEV 204

Query: 183 EDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFYAISLEGQ 242
           E C+R ID INH+ WRPS+++LKE+G D+S LK T  S  P+R+ V ENGI YAISLEGQ
Sbjct: 205 ESCLRSIDGINHINWRPSLDVLKEDGFDISSLKQTQSSTLPQRSMVVENGISYAISLEGQ 264

Query: 243 KTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSLPALELAK 302
           KTGFY DQRENRHFISTIS G++VLDLCCYSGGF+LNAARGGA +V GIDSSLPALELA+
Sbjct: 265 KTGFYTDQRENRHFISTISAGKRVLDLCCYSGGFALNAARGGATSVIGIDSSLPALELAR 324

Query: 303 ENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKK----------- 362
           ENVILN+MDP+++ F K D+T FMK ALSR E WDIVILDPPKLAPRKK           
Sbjct: 325 ENVILNNMDPEKVVFFKQDSTEFMKGALSREETWDIVILDPPKLAPRKKVLHNASGMYRN 384

Query: 363 ---------------------GAASMAKKQITVL--------------RQAGAACDHPID 394
                                GA + + K + +L              R+AGAA DHP+D
Sbjct: 385 LNSLAMRLTRSGGLMMTCSCSGAMTQSGKFLGILQSAAAMSGRKITVVREAGAASDHPLD 444

BLAST of CmaCh20G002870 vs. NCBI nr
Match: gi|449444284|ref|XP_004139905.1| (PREDICTED: uncharacterized protein LOC101215914 isoform X1 [Cucumis sativus])

HSP 1 Score: 688.0 bits (1774), Expect = 1.0e-194
Identity = 359/439 (81.78%), Postives = 378/439 (86.10%), Query Frame = 1

Query: 1   MQQLQARLLKSLSISSSPAASTIQEIASSRPKGLSKVVLKKGKTQLFKDGSPMVYSGAVD 60
           MQ LQ+RL+KSLSISSSPAASTIQEIAS+ PKGL+KVVLKKGK QLFKDGSPMVYSGAVD
Sbjct: 1   MQLLQSRLIKSLSISSSPAASTIQEIASACPKGLAKVVLKKGKAQLFKDGSPMVYSGAVD 60

Query: 61  RIIGRPPPKTGDVVLVADGAEKPIGWGLYNSVSMFSVRLMQLEEEAARDPSCTLDMQKLI 120
           RIIGRPPPKTGDVVLVADG EKPIGWGLYNSVSMFSVRLMQLEEEAARDPSCTL+++K+I
Sbjct: 61  RIIGRPPPKTGDVVLVADGTEKPIGWGLYNSVSMFSVRLMQLEEEAARDPSCTLNVEKMI 120

Query: 121 ETRIHIARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKA 180
           ETR+H ARELRK+LGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKA
Sbjct: 121 ETRVHTARELRKNLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKA 180

Query: 181 EIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFYAISLE 240
           EIEDCVRGI+EINHVKWRPSV+ILKEEGIDVSELKDT+PS CPERTKV EN IFYAISLE
Sbjct: 181 EIEDCVRGIEEINHVKWRPSVDILKEEGIDVSELKDTSPSTCPERTKVMENDIFYAISLE 240

Query: 241 GQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSLPALEL 300
           GQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNA RGGAINVTGIDSSLPALEL
Sbjct: 241 GQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNALRGGAINVTGIDSSLPALEL 300

Query: 301 AKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKK---GAASMA 360
           AKENV+LNSMDP+RISFLKADAT FMKDALSRNE WD+VILDPPKLAPRKK    A+ M 
Sbjct: 301 AKENVVLNSMDPERISFLKADATAFMKDALSRNESWDVVILDPPKLAPRKKVLQTASGMY 360

Query: 361 KK------QIT-------------------------------------VLRQAGAACDHP 394
           +       Q+T                                     VLRQAGAACDHP
Sbjct: 361 RNLNSLAMQLTRKGGFLMTCSCSGAMTQSGMFLQILQGAASTAKKKITVLRQAGAACDHP 420

BLAST of CmaCh20G002870 vs. NCBI nr
Match: gi|659112617|ref|XP_008456287.1| (PREDICTED: uncharacterized protein LOC103496280 isoform X1 [Cucumis melo])

HSP 1 Score: 679.1 bits (1751), Expect = 4.6e-192
Identity = 355/439 (80.87%), Postives = 375/439 (85.42%), Query Frame = 1

Query: 1   MQQLQARLLKSLSISSSPAASTIQEIASSRPKGLSKVVLKKGKTQLFKDGSPMVYSGAVD 60
           M  LQ+RL+KSLSISSSPAASTIQEIA++ PKGL+KVVLKKGK QLFKDGSPMVYSGAVD
Sbjct: 1   MLHLQSRLIKSLSISSSPAASTIQEIATACPKGLAKVVLKKGKAQLFKDGSPMVYSGAVD 60

Query: 61  RIIGRPPPKTGDVVLVADGAEKPIGWGLYNSVSMFSVRLMQLEEEAARDPSCTLDMQKLI 120
           RIIGRPPPKTGDVVLVADG EKPIGWGLYNSVSMFSVRLMQLEEEAA DPSCTL+++K+I
Sbjct: 61  RIIGRPPPKTGDVVLVADGTEKPIGWGLYNSVSMFSVRLMQLEEEAASDPSCTLNVEKMI 120

Query: 121 ETRIHIARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKA 180
           ETRIH ARELRK+LGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKA
Sbjct: 121 ETRIHTARELRKNLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKA 180

Query: 181 EIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFYAISLE 240
           EIEDCVRGI+EINHVKWRPSV+ILKEEGIDVSELK+T+PS CPERTKV ENGIFY ISLE
Sbjct: 181 EIEDCVRGIEEINHVKWRPSVDILKEEGIDVSELKETSPSTCPERTKVKENGIFYTISLE 240

Query: 241 GQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSLPALEL 300
           GQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNA RGGAINVTGIDSSLPALEL
Sbjct: 241 GQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNALRGGAINVTGIDSSLPALEL 300

Query: 301 AKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKK---GAASMA 360
           AKENV+LNSMDP+RISFLKADAT FMKDALSRNE WDIVILDPPKLAPRKK    A+ M 
Sbjct: 301 AKENVVLNSMDPERISFLKADATAFMKDALSRNESWDIVILDPPKLAPRKKVLQTASGMY 360

Query: 361 KK------QIT-------------------------------------VLRQAGAACDHP 394
           +       Q+T                                     VLR AGAACDHP
Sbjct: 361 RNLNSLAMQLTRKGGFLMTCSCSGAMTQSGMFLQILQGAASMAKKKITVLRPAGAACDHP 420

BLAST of CmaCh20G002870 vs. NCBI nr
Match: gi|778713034|ref|XP_011656973.1| (PREDICTED: uncharacterized protein LOC101215914 isoform X2 [Cucumis sativus])

HSP 1 Score: 646.4 bits (1666), Expect = 3.3e-182
Identity = 324/351 (92.31%), Postives = 340/351 (96.87%), Query Frame = 1

Query: 1   MQQLQARLLKSLSISSSPAASTIQEIASSRPKGLSKVVLKKGKTQLFKDGSPMVYSGAVD 60
           MQ LQ+RL+KSLSISSSPAASTIQEIAS+ PKGL+KVVLKKGK QLFKDGSPMVYSGAVD
Sbjct: 1   MQLLQSRLIKSLSISSSPAASTIQEIASACPKGLAKVVLKKGKAQLFKDGSPMVYSGAVD 60

Query: 61  RIIGRPPPKTGDVVLVADGAEKPIGWGLYNSVSMFSVRLMQLEEEAARDPSCTLDMQKLI 120
           RIIGRPPPKTGDVVLVADG EKPIGWGLYNSVSMFSVRLMQLEEEAARDPSCTL+++K+I
Sbjct: 61  RIIGRPPPKTGDVVLVADGTEKPIGWGLYNSVSMFSVRLMQLEEEAARDPSCTLNVEKMI 120

Query: 121 ETRIHIARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKA 180
           ETR+H ARELRK+LGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKA
Sbjct: 121 ETRVHTARELRKNLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKA 180

Query: 181 EIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFYAISLE 240
           EIEDCVRGI+EINHVKWRPSV+ILKEEGIDVSELKDT+PS CPERTKV EN IFYAISLE
Sbjct: 181 EIEDCVRGIEEINHVKWRPSVDILKEEGIDVSELKDTSPSTCPERTKVMENDIFYAISLE 240

Query: 241 GQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSLPALEL 300
           GQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNA RGGAINVTGIDSSLPALEL
Sbjct: 241 GQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNALRGGAINVTGIDSSLPALEL 300

Query: 301 AKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKK 352
           AKENV+LNSMDP+RISFLKADAT FMKDALSRNE WD+VILDPPKLAPRKK
Sbjct: 301 AKENVVLNSMDPERISFLKADATAFMKDALSRNESWDVVILDPPKLAPRKK 351

BLAST of CmaCh20G002870 vs. NCBI nr
Match: gi|778713038|ref|XP_011656974.1| (PREDICTED: uncharacterized protein LOC101215914 isoform X3 [Cucumis sativus])

HSP 1 Score: 646.4 bits (1666), Expect = 3.3e-182
Identity = 324/351 (92.31%), Postives = 340/351 (96.87%), Query Frame = 1

Query: 1   MQQLQARLLKSLSISSSPAASTIQEIASSRPKGLSKVVLKKGKTQLFKDGSPMVYSGAVD 60
           MQ LQ+RL+KSLSISSSPAASTIQEIAS+ PKGL+KVVLKKGK QLFKDGSPMVYSGAVD
Sbjct: 1   MQLLQSRLIKSLSISSSPAASTIQEIASACPKGLAKVVLKKGKAQLFKDGSPMVYSGAVD 60

Query: 61  RIIGRPPPKTGDVVLVADGAEKPIGWGLYNSVSMFSVRLMQLEEEAARDPSCTLDMQKLI 120
           RIIGRPPPKTGDVVLVADG EKPIGWGLYNSVSMFSVRLMQLEEEAARDPSCTL+++K+I
Sbjct: 61  RIIGRPPPKTGDVVLVADGTEKPIGWGLYNSVSMFSVRLMQLEEEAARDPSCTLNVEKMI 120

Query: 121 ETRIHIARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKA 180
           ETR+H ARELRK+LGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKA
Sbjct: 121 ETRVHTARELRKNLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKA 180

Query: 181 EIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFYAISLE 240
           EIEDCVRGI+EINHVKWRPSV+ILKEEGIDVSELKDT+PS CPERTKV EN IFYAISLE
Sbjct: 181 EIEDCVRGIEEINHVKWRPSVDILKEEGIDVSELKDTSPSTCPERTKVMENDIFYAISLE 240

Query: 241 GQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSLPALEL 300
           GQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNA RGGAINVTGIDSSLPALEL
Sbjct: 241 GQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNALRGGAINVTGIDSSLPALEL 300

Query: 301 AKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKK 352
           AKENV+LNSMDP+RISFLKADAT FMKDALSRNE WD+VILDPPKLAPRKK
Sbjct: 301 AKENVVLNSMDPERISFLKADATAFMKDALSRNESWDVVILDPPKLAPRKK 351

BLAST of CmaCh20G002870 vs. NCBI nr
Match: gi|659112619|ref|XP_008456288.1| (PREDICTED: uncharacterized protein LOC103496280 isoform X2 [Cucumis melo])

HSP 1 Score: 644.0 bits (1660), Expect = 1.6e-181
Identity = 323/352 (91.76%), Postives = 339/352 (96.31%), Query Frame = 1

Query: 1   MQQLQARLLKSLSISSSPAASTIQEIASSRPKGLSKVVLKKGKTQLFKDGSPMVYSGAVD 60
           M  LQ+RL+KSLSISSSPAASTIQEIA++ PKGL+KVVLKKGK QLFKDGSPMVYSGAVD
Sbjct: 1   MLHLQSRLIKSLSISSSPAASTIQEIATACPKGLAKVVLKKGKAQLFKDGSPMVYSGAVD 60

Query: 61  RIIGRPPPKTGDVVLVADGAEKPIGWGLYNSVSMFSVRLMQLEEEAARDPSCTLDMQKLI 120
           RIIGRPPPKTGDVVLVADG EKPIGWGLYNSVSMFSVRLMQLEEEAA DPSCTL+++K+I
Sbjct: 61  RIIGRPPPKTGDVVLVADGTEKPIGWGLYNSVSMFSVRLMQLEEEAASDPSCTLNVEKMI 120

Query: 121 ETRIHIARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKA 180
           ETRIH ARELRK+LGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKA
Sbjct: 121 ETRIHTARELRKNLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKA 180

Query: 181 EIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFYAISLE 240
           EIEDCVRGI+EINHVKWRPSV+ILKEEGIDVSELK+T+PS CPERTKV ENGIFY ISLE
Sbjct: 181 EIEDCVRGIEEINHVKWRPSVDILKEEGIDVSELKETSPSTCPERTKVKENGIFYTISLE 240

Query: 241 GQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSLPALEL 300
           GQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNA RGGAINVTGIDSSLPALEL
Sbjct: 241 GQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNALRGGAINVTGIDSSLPALEL 300

Query: 301 AKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKG 353
           AKENV+LNSMDP+RISFLKADAT FMKDALSRNE WDIVILDPPKLAPRKKG
Sbjct: 301 AKENVVLNSMDPERISFLKADATAFMKDALSRNESWDIVILDPPKLAPRKKG 352

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RLMI_ALTMD4.5e-3833.96Ribosomal RNA large subunit methyltransferase I OS=Alteromonas mediterranea (str... [more]
RLMI_PSEHT1.0e-3728.89Ribosomal RNA large subunit methyltransferase I OS=Pseudoalteromonas haloplankti... [more]
RLMI_ECOSM2.5e-3626.73Ribosomal RNA large subunit methyltransferase I OS=Escherichia coli (strain SMS-... [more]
RLMI_ECOLC5.5e-3626.73Ribosomal RNA large subunit methyltransferase I OS=Escherichia coli (strain ATCC... [more]
RLMI_ECOLI5.5e-3626.73Ribosomal RNA large subunit methyltransferase I OS=Escherichia coli (strain K12)... [more]
Match NameE-valueIdentityDescription
A0A0A0KB85_CUCSA1.8e-17181.14Uncharacterized protein OS=Cucumis sativus GN=Csa_6G135980 PE=4 SV=1[more]
M5WGM9_PRUPE5.0e-16969.93Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005882mg PE=4 SV=1[more]
A0A0D2PUQ0_GOSRA1.9e-16868.23Uncharacterized protein OS=Gossypium raimondii GN=B456_001G051600 PE=4 SV=1[more]
B9HYB4_POPTR1.6e-16769.75Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s18100g PE=4 SV=2[more]
A0A0B0NK02_GOSAR9.8e-16566.89Ribosomal RNA large subunit methyltransferase I OS=Gossypium arboreum GN=F383_01... [more]
Match NameE-valueIdentityDescription
AT1G54310.25.5e-15965.45 S-adenosyl-L-methionine-dependent methyltransferases superfamily pro... [more]
Match NameE-valueIdentityDescription
gi|449444284|ref|XP_004139905.1|1.0e-19481.78PREDICTED: uncharacterized protein LOC101215914 isoform X1 [Cucumis sativus][more]
gi|659112617|ref|XP_008456287.1|4.6e-19280.87PREDICTED: uncharacterized protein LOC103496280 isoform X1 [Cucumis melo][more]
gi|778713034|ref|XP_011656973.1|3.3e-18292.31PREDICTED: uncharacterized protein LOC101215914 isoform X2 [Cucumis sativus][more]
gi|778713038|ref|XP_011656974.1|3.3e-18292.31PREDICTED: uncharacterized protein LOC101215914 isoform X3 [Cucumis sativus][more]
gi|659112619|ref|XP_008456288.1|1.6e-18191.76PREDICTED: uncharacterized protein LOC103496280 isoform X2 [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002478PUA
IPR015947PUA-like_sf
IPR019614SAM-dep_methyl-trfase
Vocabulary: Molecular Function
TermDefinition
GO:0003723RNA binding
GO:0008168methyltransferase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0008150 biological_process
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0003723 RNA binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh20G002870.1CmaCh20G002870.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002478PUA domainSMARTSM00359pua_5coord: 35..115
score: 4.
IPR015947PUA-like domainGENE3DG3DSA:2.30.130.10coord: 36..105
score: 4.1
IPR015947PUA-like domainunknownSSF88697PUA domain-likecoord: 35..104
score: 2.9
IPR019614S-adenosylmethionine-dependent methyltransferasePFAMPF10672Methyltrans_SAMcoord: 223..366
score: 4.1
NoneNo IPR availableGENE3DG3DSA:3.30.750.80coord: 118..211
score: 1.9
NoneNo IPR availablePANTHERPTHR11061RNA M5U METHYLTRANSFERASE FAMILYcoord: 32..393
score: 5.9E
NoneNo IPR availablePANTHERPTHR11061:SF12RNA BINDING PROTEIN-RELATEDcoord: 32..393
score: 5.9E

The following gene(s) are paralogous to this gene:

None