CmaCh18G005700 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh18G005700 vs. Swiss-Prot
Match: SBT24_ARATH (Subtilisin-like protease SBT2.4 OS=Arabidopsis thaliana GN=SBT2.4 PE=1 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 1.0e-230
Identity = 426/780 (54.62%), Postives = 527/780 (67.56%), Query Frame = 1

Query: 39  DHLLQNTLK--SYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYT 98
           D +L +TL+  SYTKL SFKH++N  AV TT S+A KL +  GVK VE D+GV+ MTTYT
Sbjct: 83  DEILGSTLEKGSYTKLYSFKHVINAIAVRTTASQAKKLGKTKGVKAVEEDKGVKLMTTYT 142

Query: 99  PKFLGVPSKSRLCSKKTKTSPDGEGIVIGFVDSGIDPTHPSFG-------FDDGYEDLRL 158
           P FL +P +             GE IVIGFVD+GI+PTHPSF        +      L  
Sbjct: 143 PDFLELPQQVWQKISNEGDRRAGEDIVIGFVDTGINPTHPSFAALDLTNPYSSNLSRLHF 202

Query: 159 F--CEKGRFFPLSSCNGKIVGARFFSAGARAVAKLNSSVDFLSPFDVEGHGSHVASIAAG 218
              CE G FFP  SCNGKI+ ARFFSAGARA   LNSS+D LSPFD  GHGSHVASIAAG
Sbjct: 203 SGDCEIGPFFPPGSCNGKIISARFFSAGARASGALNSSLDILSPFDASGHGSHVASIAAG 262

Query: 219 NGGVPVYVNGFFYGFASGMAPRARIAVYKAVYPTMSTLTDVVSAIDQAVIDGVDILALSV 278
           N GVPV V+GFFYG ASGMAPR+RIAVYKA+YP++ TL DV++AIDQA++DGVD+L LSV
Sbjct: 263 NAGVPVIVDGFFYGRASGMAPRSRIAVYKAIYPSIGTLVDVIAAIDQAIMDGVDVLTLSV 322

Query: 279 GPNEPPEEGLTFLSIYDIAILSATRAGILVVQAVGNNGPASATVVSYSPWAVGVAASGTD 338
           GP+EPP +  T L I+D+A+L A +AG+ VVQAVGNNGP+ ++V+SYSPW VGVAA  TD
Sbjct: 323 GPDEPPVDKPTVLGIFDLAMLLARKAGVFVVQAVGNNGPSPSSVLSYSPWVVGVAAGNTD 382

Query: 339 RVYPASLLLGNGQKVGGVGLSGGSIHFCGYIFWDLYYSSQSNFLNKGFPLYSKHLSSSSG 398
           R YPA L+L  GQ V GVGLSG ++                     G PL    L  +  
Sbjct: 383 RSYPAPLILDGGQTVQGVGLSGPTL---------------------GAPLVQHRLVLAKD 442

Query: 399 PTFFLHKLVLAKDAMKQNGTIPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAI 458
                  ++       +    P         FDP  V  SIV+C+FS GF N  S++ AI
Sbjct: 443 AVRTNGSVLQPLTRDIEECQRPEN-------FDPAAVFGSIVICTFSDGFYNQMSTVLAI 502

Query: 459 IHTAMTLRFIGFALIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDANG 518
             TA TL F+GF LIANP +GD++AEP+ F  PGILIP  S +Q IL YYE  T +D  G
Sbjct: 503 TQTARTLGFMGFILIANPRFGDYVAEPVIFSAPGILIPTVSAAQIILRYYEEKTFRDTRG 562

Query: 519 MVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWA 578
           + ++F  +A IGEGR + F  +AP VSRFSSRGP +++  R+  DVLKPDILAPGHQIW 
Sbjct: 563 VATQFGARARIGEGRNSVFAGKAPVVSRFSSRGPAFIDATRSPLDVLKPDILAPGHQIWG 622

Query: 579 AWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKY 638
           AWS  S  +P+L G  FA++SGTSMA PHI GI ALIKQ NPSWTP+MIASA+STTA +Y
Sbjct: 623 AWSLPSAFDPILTGRSFAILSGTSMATPHIAGIGALIKQLNPSWTPAMIASAISTTANEY 682

Query: 639 DMNGDLIQAEGFDIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAEYEDYINFLCSLPG-D 698
           D NG++I AE +++ RL+PS  FD GAG V P  ALDPGLV PA +EDYI+FLCSLP   
Sbjct: 683 DSNGEIISAEYYELSRLFPSNHFDHGAGHVNPARALDPGLVLPAGFEDYISFLCSLPNIS 742

Query: 699 PAVIKNTTGGQCNASVPQPHPADLNLPSVTISSLVGHQVLHRRVKNVGTKVETYVWSVIP 758
           PA I++ TG  C  ++   HPA+LN PSVTIS+L    V+ R  ++V  K ETY+ SV+P
Sbjct: 743 PATIRDATGVLCTTTL--SHPANLNHPSVTISALKESLVVRRSFQDVSNKTETYLGSVLP 802

Query: 759 PNGTTININPPWFTIAPGGLQNLEIQIKVTHKMNHFTFGEILFTGSLNHIGRIPLSVLAV 807
           PNGTT+ + P WFT+ P   Q+L+I+  VT  +N FTFGE++ TGSLNHI RIPLSV  +
Sbjct: 803 PNGTTVRLTPTWFTVPPQKTQDLDIEFNVTQVLNKFTFGEVVLTGSLNHIIRIPLSVKTI 832

BLAST of CmaCh18G005700 vs. Swiss-Prot
Match: SBT25_ARATH (Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana GN=SBT2.5 PE=2 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 3.8e-164
Identity = 337/768 (43.88%), Postives = 456/768 (59.38%), Query Frame = 1

Query: 48  SYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSR 107
           SY KL S+KH++NGFA H +P +A  LR+A GV+ V++D  VR++TT+TP+FLG+P+   
Sbjct: 82  SYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRLTTHTPEFLGLPTD-- 141

Query: 108 LCSKKTKTSPDGEGIVIGFVDSGIDPTHPSFGFDDG--YEDLRLF---CEKGRFFPLSSC 167
           +          GE IVIGFVDSGI P HPSF       Y  L  +   CE+      S C
Sbjct: 142 VWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLPHYKGKCEEDPHTKKSFC 201

Query: 168 NGKIVGARFFSAGARAVAKLNSSVDFLSPFDVEGHGSHVASIAAGNGGVPVYVNGFFYGF 227
           N KIVGA+ F+  A+A    N  +D+ SP D +GHGSH A+IAAGN G+P+ ++G+ +G 
Sbjct: 202 NRKIVGAQHFAEAAKAAGAFNPDIDYASPMDGDGHGSHTAAIAAGNNGIPLRMHGYEFGK 261

Query: 228 ASGMAPRARIAVYKAVYPTMST-LTDVVSAIDQAVIDGVDILALSVGPNEPPEEG-LTFL 287
           ASGMAPRARIAVYKA+Y      + DVV+AIDQAV DGVDIL+LSVGPN PP     TFL
Sbjct: 262 ASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPTTTKTTFL 321

Query: 288 SIYDIAILSATRAGILVVQAVGNNGPASATVVSYSPWAVGVAASGTDRVYPASLLLGNGQ 347
           + +D  +L A +AG+ V QA GN GP   T+VSYSPW   VAA+  DR Y   L LGNG+
Sbjct: 322 NPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDDRRYKNHLTLGNGK 381

Query: 348 KVGGVGLSGGSIHFCGYIFWDLYYSSQSNFLNKGFPLYSKHLSSSSGPTFFLHKLVLAKD 407
            + G+GLS  +                     +   LY+  L S+       + ++L   
Sbjct: 382 MLAGMGLSPPT---------------------RPHRLYT--LVSA-------NDVLLDSS 441

Query: 408 AMKQNGTIPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAIIHTAMTLRFIGFA 467
             K N +    +CQ PE F+  LV+ +I+LC +S  F+ GT+S+  ++ TA  L   GF 
Sbjct: 442 VSKYNPS----DCQRPEVFNKKLVEGNILLCGYSFNFVVGTASIKKVVATAKHLGAAGFV 501

Query: 468 LIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDANGMVSEFKGKAAIGE 527
           L+          +P+   +PGILI + S S  +++YY A+T +D  G V  FK + +IG+
Sbjct: 502 LVVENVSPGTKFDPVPSAIPGILITDVSKSMDLIDYYNASTSRDWTGRVKSFKAEGSIGD 561

Query: 528 GRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTEPLLK 587
           G        AP V+ FS+RGP+  + +   AD+LKPDILAPG+ IWAAW P    EP   
Sbjct: 562 GLAPVLHKSAPQVALFSARGPNTKDFSFQDADLLKPDILAPGYLIWAAWCPNGTDEPNYV 621

Query: 588 GYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQAEGF- 647
           G  FAL+SGTSMAAPHI GIAAL+KQK+P W+P+ I SA+ TT+T  D  G L+QA+ + 
Sbjct: 622 GEGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVIDRAGRLLQAQQYS 681

Query: 648 --DIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAEYEDYINFLCSLPGDPA-VIKNTTGG 707
             +   L  +TPFD G+G V P+ ALDPGL+F A YEDY+ FLC+ PG  A  I+N T  
Sbjct: 682 DTEAVTLVKATPFDYGSGHVNPSAALDPGLIFDAGYEDYLGFLCTTPGISAHEIRNYTNT 741

Query: 708 QCNASVPQPHPADLNLPSVTISSLVGHQVLHRRVKNVGTKVETYVWSVIPPNGTTININP 767
            CN  +   HP++ N PS+ +S LVG Q + R+V NV    ETY  +        I +NP
Sbjct: 742 ACNYDM--KHPSNFNAPSIAVSHLVGTQTVTRKVTNVAEVEETYTITARMQPSIAIEVNP 801

Query: 768 PWFTIAPGGLQNLEIQIKVTHKMNHFTFGEILFTGSLNHIGRIPLSVL 805
           P  T+ PG  +   + + V      ++FGE+   GS  H  RIP+  L
Sbjct: 802 PAMTLRPGATRTFSVTMTVRSVSGVYSFGEVKLKGSRGHKVRIPVVAL 811

BLAST of CmaCh18G005700 vs. Swiss-Prot
Match: SBT26_ARATH (Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana GN=SBT2.6 PE=2 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 3.1e-158
Identity = 331/773 (42.82%), Postives = 448/773 (57.96%), Query Frame = 1

Query: 48  SYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSR 107
           SY KL S+KH++NGFA H +P +A  LR+A GVK V+RD  VRK+TT+TP+FLG+P+   
Sbjct: 82  SYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRDWKVRKLTTHTPQFLGLPTD-- 141

Query: 108 LCSKKTKTSPDGEGIVIGFVDSGIDPTHPSFGFDDG------YEDLRLFCEKGRFFPLSS 167
           +          GE IVIGF+DSGI P HPSF           +   +  CE+     +S 
Sbjct: 142 VWPTGGGYDRAGEDIVIGFIDSGIFPHHPSFASHHTTVPYGPHPSYKGKCEEDPHTKISF 201

Query: 168 CNGKIVGARFFSAGARAVAKLNSSVDFLSPFDVEGHGSHVASIAAGNGGVPVYVNGFFYG 227
           CNGKI+GA+ F+  A+A    N  +DF SP D +GHGSH A+IAAGN G+PV ++G+ +G
Sbjct: 202 CNGKIIGAQHFAEAAKAAGAFNPDIDFASPMDGDGHGSHTAAIAAGNNGIPVRMHGYEFG 261

Query: 228 FASGMAPRARIAVYKAVYPTMST-LTDVVSAIDQAVIDGVDILALSVGPNEPPEEG-LTF 287
            ASGMAPRARIAVYKA+Y      + DVV+AIDQAV DGVDIL+LSVGPN PP     TF
Sbjct: 262 KASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPATTKTTF 321

Query: 288 LSIYDIAILSATRAGILVVQAVGNNGPASATVVSYSPWAVGVAASGTDRVYPASLLLGNG 347
           L+ +D  +L A +AG+ V QA GN GP   T+VSYSPW   VAA+  DR Y   L LGNG
Sbjct: 322 LNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDDRRYKNHLTLGNG 381

Query: 348 QKVGGVGLSGGSIHFCGYIFWDLYYSSQSNFLNKGFPLYSKH--LSSSSGPTFFLHKLVL 407
           + + G+GL                  S S   ++ + + S +  L  SSG  +       
Sbjct: 382 KMLAGIGL------------------SPSTRPHRSYKMVSANDVLLGSSGMKY------N 441

Query: 408 AKDAMKQNGTIPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAIIHTAMTLRFI 467
             D  K            PE  +  LV+ +I+LC +S  F+ G++S+  +  TA  L   
Sbjct: 442 PSDCQK------------PEVLNKKLVEGNILLCGYSFNFVAGSASIKKVAETAKHLGAA 501

Query: 468 GFALIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDANGMVSEFKGKAA 527
           GF L+          +P+   +PGILI + S S  +++YY   T +D  G V +FK + +
Sbjct: 502 GFVLVVENVSPGTKFDPVPSCIPGILITDVSKSMDLIDYYNVTTSRDWMGRVKDFKAEGS 561

Query: 528 IGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTEP 587
           IG+G        AP V+ FS+RGP+  + +   AD+LKPDILAPG  IW+AWS     E 
Sbjct: 562 IGDGLEPILHKSAPEVALFSARGPNTKDFSFQDADLLKPDILAPGSLIWSAWSANGTDEA 621

Query: 588 LLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQAE 647
              G  FAL+SGTSMAAPHI GIAAL+KQK+P W+P+ I SA+ TT+T  D  G  +QA+
Sbjct: 622 NYIGEGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVIDRAGRPLQAQ 681

Query: 648 GF---DIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAEYEDYINFLCSLPG-DPAVIKNT 707
            +   +   L  +TPFD G+G V P+ ALDPGL+F A YEDYI FLC+ PG D   IKN 
Sbjct: 682 QYSETETVTLVKATPFDYGSGHVNPSAALDPGLIFDAGYEDYIGFLCTTPGIDAHEIKNF 741

Query: 708 TGGQCNASVPQPHPADLNLPSVTISSLVGHQVLHRRVKNVGTKVETYVWSVIPPNGTTIN 767
           T   CN  +   HP++ N PS+ IS LV  Q + RRV NV  + ETY  +        I 
Sbjct: 742 TNTPCNFKM--VHPSNFNTPSIAISHLVRTQTVTRRVTNVAEEEETYTITSRMEPAIAIE 801

Query: 768 INPPWFTIAPGGLQNLEIQIKVTHKMNHFTFGEILFTGSLNHIGRIPLSVLAV 807
           ++PP  T+  G  +   + + V      ++FG++   GS  H  ++ L V+A+
Sbjct: 802 VSPPAMTVRAGASRTFSVTLTVRSVTGAYSFGQVTLKGSRGH--KVTLPVVAM 812

BLAST of CmaCh18G005700 vs. Swiss-Prot
Match: SBT21_ARATH (Subtilisin-like protease SBT2.1 OS=Arabidopsis thaliana GN=SBT2.1 PE=2 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.1e-142
Identity = 315/801 (39.33%), Postives = 449/801 (56.05%), Query Frame = 1

Query: 22  AEERQIYLVLMEESSN---EDHLLQNTLK--SYTKLQSFKHIMNGFAVHTTPSEAAKLRQ 81
           A   QIY  L   +S     D LL+N L+  +Y KL S+ +++NGF+   T  +A +L  
Sbjct: 63  ATSSQIYRTLNRSASIIRVHDSLLRNVLRKENYLKLYSYHYLINGFSAVLTRKQADRLAA 122

Query: 82  ANGVKLVERDRGVRKMTTYTPKFLGVPSKSRLCSKKTKTSPDGEGIVIGFVDSGIDPTHP 141
              V+ V  D  V K TT+TP+FLG+P  + L    ++ +  GEG+VIGF+D+GIDPTHP
Sbjct: 123 REEVENVVLDFLVEKATTHTPQFLGLPRGAWLRDGGSEYA--GEGVVIGFIDTGIDPTHP 182

Query: 142 SFGFDDGYEDLRL------FCEKGRFFPLSSCNGKIVGARFFSAGARAVAKLNSSVDFLS 201
           SF          +       CE    FP  SCN K++GAR F+  A +   LNSS D  S
Sbjct: 183 SFSDKISGHTYSVPPHFTGVCEVTIGFPPGSCNRKLIGARHFAESALSRGVLNSSQDDAS 242

Query: 202 PFDVEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAVYPTMSTL-TDVV 261
           PFD EGHG+H AS+AAGN G+PV V G   G ASGMAPRA IA+YKA+Y        D++
Sbjct: 243 PFDGEGHGTHTASVAAGNHGIPVVVAGHRLGNASGMAPRAHIAIYKALYKRFGGFAADII 302

Query: 262 SAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVVQAVGNNGPASA 321
           +AIDQA  DGVDI+ LS+ PN  P    TF +  D+A+LSA +AGI VVQA GN GPA  
Sbjct: 303 AAIDQAAQDGVDIINLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPK 362

Query: 322 TVVSYSPWAVGVAASGTDRVYPASLLLGNGQKVGGVGLSGGSIHFCGYIFWDLYYSSQSN 381
           ++ S+SPW   V A+  DRVY  S++LGN   + GVGL+ G+      I   L  ++ + 
Sbjct: 363 SMSSFSPWIFTVGATSHDRVYSNSIILGNNVTIPGVGLASGT-----RIMHKLVLATHA- 422

Query: 382 FLNKGFPLYSKHLSSSSGPTFFLHKLVLAKDAMKQNGTIPSEECQYPEAFDPNLVQNSIV 441
             N    + + ++      + F  KLV                            Q  I+
Sbjct: 423 LRNGTTVMDAIYVGECQDSSSFDQKLV----------------------------QGKIL 482

Query: 442 LCSFSQGFLNGTSSLAAIIHTAMTLRFIGFALIANPNYGDFIAEPITFRLPGILIPNASD 501
           +CS++  F+ G S++   + TA  L   G     +P+   F        +PGILI +  D
Sbjct: 483 VCSYTVRFILGVSTIKQALLTAKNLTAAGLVFYIDPSATGFQMTSSPMDIPGILISSPQD 542

Query: 502 SQAILNYYEANTCKD-ANGMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNR 561
           SQA+L YY ++  ++  +G +      A I  G   ++G  AP V  FS+RGPD  + + 
Sbjct: 543 SQALLRYYNSSLLRENGSGKIVGSASVAKIVGGMRPTYGITAPKVMYFSARGPDPEDDSF 602

Query: 562 TVADVLKPDILAPGHQIWAAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKN 621
             AD++KP+++APG+ IW AWSPL +     +G +FA+ SGTSM+APH+ GIAALIKQK 
Sbjct: 603 VDADIMKPNLVAPGNAIWGAWSPLGIGTNDFQGERFAMESGTSMSAPHVTGIAALIKQKF 662

Query: 622 PSWTPSMIASAMSTTATKYDMNGDLIQAEGFDIH---RLYPSTPFDLGAGLVKPTNALDP 681
           P +TP+ IASA+STTA+  D  G+ I A+   ++      P+TPFD+G+G V  T ALDP
Sbjct: 663 PHFTPAAIASALSTTASLSDRKGEHIMAQRTVLNPDISQSPATPFDMGSGFVNATAALDP 722

Query: 682 GLVFPAEYEDYINFLCSLPGDPAVIKNTTGGQCNASVPQPHPADLNLPSVTISSLVGHQV 741
           GL+F   Y +Y+ FLC + G   V+ N TG  C++       +DLNLPSVTI+ LVG + 
Sbjct: 723 GLIFDIGYNEYMKFLCGINGSSPVVLNYTGESCSSYNSSLAASDLNLPSVTIAKLVGTRA 782

Query: 742 LHRRVKNVGTKV--ETYVWSVIPPNGTTININPPWFTIAPGGLQNLEIQIKVTHKMNHFT 801
           + R V N+ T    ETY+   + P+  ++ ++P  FTI  G  + L +  +    ++  +
Sbjct: 783 VLRWVTNIATTATNETYIVGWMAPDSVSVKVSPAKFTIGNGQTRVLSLVFRAMKNVSMAS 827

Query: 802 FGEILFTGSLNHIGRIPLSVL 805
           FG I   G   H+  IP++V+
Sbjct: 843 FGRIGLFGDRGHVVNIPVAVI 827

BLAST of CmaCh18G005700 vs. Swiss-Prot
Match: SBT23_ARATH (Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana GN=SBT2.3 PE=2 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 4.4e-80
Identity = 167/422 (39.57%), Postives = 243/422 (57.58%), Query Frame = 1

Query: 392 LHKLVLAKDAMKQNGTIPSE----ECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAI 451
           ++K++ A  A+  + ++  +    ECQ  E FD + V   +++CS+S  F+ G S++   
Sbjct: 415 MYKMISAFHALNNSTSVDKDMYVGECQDYENFDQDRVSGKLLICSYSARFVLGLSTIKQA 474

Query: 452 IHTAMTLRFIGFALIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDANG 511
           +  A  L   G     +P    F   P    +PGI+IP+  DS+ +L YY ++  +D   
Sbjct: 475 LDVAKNLSATGVIFYIDPYVLGFEINPTPMDMPGIIIPSVEDSKTLLKYYNSSIQRDVTT 534

Query: 512 M-VSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIW 571
             +  F   AAI  G  A+F ++AP V  +S+RGPD  + +   ADVLKP+++APG+ IW
Sbjct: 535 KEIVSFGAVAAIEGGLNANFSNRAPKVMYYSARGPDPEDNSFNDADVLKPNLVAPGNSIW 594

Query: 572 AAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATK 631
            AWS  S      +G +FA+MSGTSMAAPH+ G+AALIKQ  P +TPS I+SA+STTA  
Sbjct: 595 GAWSSASTDSTEFEGEKFAMMSGTSMAAPHVAGVAALIKQSYPQFTPSTISSALSTTALL 654

Query: 632 YDMNGDLIQAE---GFDIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAEYEDYINFLCSL 691
            D  G  I A+         LY +TP D+G+G V  T ALDPGLVF   +EDYI+FLC +
Sbjct: 655 NDNKGSPIMAQRTYSNPDQSLYTATPSDMGSGFVNATAALDPGLVFDTSFEDYISFLCGI 714

Query: 692 PGDPAVIKNTTGGQCNASVPQPHPADLNLPSVTISSLVGHQVLHRRVKNVGTKVETYVWS 751
            G   V+ N TG +C A+       DLNLPS+T+S+L G Q   R ++N+    ETY   
Sbjct: 715 NGSDTVVFNYTGFRCPANNTPVSGFDLNLPSITVSTLSGTQTFQRSMRNIAGN-ETYNVG 774

Query: 752 VIPPNGTTININPPWFTIAPGGLQNLEIQIKVTHKMNHFTFGEILFTGSLNHIGRIPLSV 806
             PP G ++ ++P  F+IA G  Q L + + VT   +  +FG I   G+  HI  IP++V
Sbjct: 775 WSPPYGVSMKVSPTQFSIAMGENQVLSVTLTVTKNSSSSSFGRIGLFGNTGHIVNIPVTV 834

BLAST of CmaCh18G005700 vs. TrEMBL
Match: B9RXF5_RICCO (Subtilase, putative OS=Ricinus communis GN=RCOM_0903320 PE=3 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 7.2e-271
Identity = 501/844 (59.36%), Postives = 609/844 (72.16%), Query Frame = 1

Query: 6   STLSGLIASLVLVYCFAEERQIYLVLME----------------------ESS------- 65
           ST S L+  ++ V C AEER IYLVLME                      E+S       
Sbjct: 6   STYSLLLVFVISVACSAEERSIYLVLMEGQPVAFLGGHEPYTTRKLELNSEASQAHARRL 65

Query: 66  --NEDHLLQNTLK--SYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKM 125
             + D LLQ+TL+  SYTKL SFKHI+NGFAVH T S+A KL+ A GVK+VERDRG + M
Sbjct: 66  VDSHDQLLQSTLEIGSYTKLYSFKHIVNGFAVHATHSQAKKLKDAPGVKVVERDRGAKLM 125

Query: 126 TTYTPKFLGVPSKSRLCSKKTKTSPDGEGIVIGFVDSGIDPTHPSFG------FDDGYED 185
           TTYTP+FL +     + +++      GEGIVIGF+D+GI+P HPSF       F      
Sbjct: 126 TTYTPQFLEL--SQGVWTQEGGDRNAGEGIVIGFIDTGINPLHPSFAYNPLNPFTSNISH 185

Query: 186 LRLFCEKGRFFPLSSCNGKIVGARFFSAGARAVAKLNSSVDFLSPFDVEGHGSHVASIAA 245
               CE G  FP  SCNGKIV ARFFSAGA+AV+ LN+S+DFLSP+D  GHGSHVAS AA
Sbjct: 186 FSGACETGPRFPAGSCNGKIVSARFFSAGAQAVSPLNTSLDFLSPYDAVGHGSHVASTAA 245

Query: 246 GNGGVPVYVNGFFYGFASGMAPRARIAVYKAVYPTMSTLTDVVSAIDQAVIDGVDILALS 305
           GN  VPV  NGF+YG ASGMAPRARIAVYKA+YPT+ TLTDV++AIDQA  DGVDI+ LS
Sbjct: 246 GNARVPVVANGFYYGRASGMAPRARIAVYKAIYPTVGTLTDVIAAIDQATKDGVDIITLS 305

Query: 306 VGPNEPPEEGLTFLSIYDIAILSATRAGILVVQAVGNNGPASATVVSYSPWAVGVAASGT 365
           VGP+EPPE+ +TFLS++D+ +L A RAG+ VVQA GN+GP+ +TVVSYSPWAVGVAAS T
Sbjct: 306 VGPDEPPEDTITFLSVFDVFMLFAQRAGVFVVQAAGNHGPSLSTVVSYSPWAVGVAASTT 365

Query: 366 DRVYPASLLLGNGQKVGGVGLSGGSIHFCGYIFWDLYYSSQSNFLNKGFPLYSKHLSSSS 425
           DR+YPASLLLGNGQKVGGVGLSG +  + G   + L ++  +   N  FP   +++    
Sbjct: 366 DRIYPASLLLGNGQKVGGVGLSGPTFGY-GLFKYKLVFAQDAVKANGTFPRTPQYI---- 425

Query: 426 GPTFFLHKLVLAKDAMKQNGTIPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAA 485
                                   EECQ+PE+ DP LV+  IV+C+FS GF NGTSS+ A
Sbjct: 426 ------------------------EECQHPESLDPKLVRRRIVICTFSAGFYNGTSSITA 485

Query: 486 IIHTAMTLRFIGFALIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDAN 545
           II T+ TLRF GFAL+ANP+YGDFIAEPI F +PGI+IP  +D++ I  YYE    +D  
Sbjct: 486 IIDTSRTLRFTGFALVANPSYGDFIAEPIPFAVPGIMIPKVADAEIISKYYEQEILRDER 545

Query: 546 GMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIW 605
           G VS+F  + AIGEGRVA+F  +AP VSRFSSRGPD++++NR  ADVLKPDILAPGHQIW
Sbjct: 546 GFVSKFCARGAIGEGRVAAFEGRAPIVSRFSSRGPDFLDINRIPADVLKPDILAPGHQIW 605

Query: 606 AAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATK 665
           AAWSPLS  +P+L G  FAL+SGTSMA PHIVGIAALIKQ +PSWTPSMIASA+STTA  
Sbjct: 606 AAWSPLSALDPILTGDNFALLSGTSMATPHIVGIAALIKQFHPSWTPSMIASALSTTAGN 665

Query: 666 YDMNGDLIQAEGFDIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAEYEDYINFLCSLPG- 725
           YD  G+LI AEGFDI+ LYPST FDLGAG V PT A+DPGLVFP+E+++YI+FLCSLPG 
Sbjct: 666 YDNYGELILAEGFDINSLYPSTHFDLGAGFVNPTRAMDPGLVFPSEFQNYISFLCSLPGI 725

Query: 726 DPAVIKNTTGGQCNASVPQPHPADLNLPSVTISSLVGHQVLHRRVKNVGTKVETYVWSVI 785
           DPA++K TTG  CN S+    PA+LNLPSVTIS+L G Q + R VKNVG K ETY+ SVI
Sbjct: 726 DPAIVKATTGEPCNQSLSS--PANLNLPSVTISALRGSQTVERNVKNVGIKPETYLSSVI 785

Query: 786 PPNGTTININPPWFTIAPGGLQNLEIQIKVTHKMNHFTFGEILFTGSLNHIGRIPLSVLA 810
            PNGTT+N++P WF IAP G QN++I+  VTH  N F+FG+I+ TGSL+HI RIPLS+L 
Sbjct: 786 APNGTTVNLSPTWFIIAPQGTQNIDIEFHVTHARNEFSFGQIVLTGSLDHIVRIPLSILP 816

BLAST of CmaCh18G005700 vs. TrEMBL
Match: W9RY25_9ROSA (Subtilisin-like protease OS=Morus notabilis GN=L484_014157 PE=3 SV=1)

HSP 1 Score: 935.3 bits (2416), Expect = 5.1e-269
Identity = 496/845 (58.70%), Postives = 608/845 (71.95%), Query Frame = 1

Query: 10  GLIASLVLVYCFAEERQIYLVLMEES-----------------------------SNEDH 69
           GL+  ++   C AEER +YLV ME+                              ++ D 
Sbjct: 16  GLLMVIMTADCLAEERALYLVTMEDEPAAFLGGSNLSHKGHKHSEVSRAHAKRLVNSHDK 75

Query: 70  LLQNTLK--SYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPK 129
           LLQ+TL+  SYTKL SFKHI+NGFAVHTTPS+A+KLR   GV+LVE+DRG + MTTYTPK
Sbjct: 76  LLQSTLETGSYTKLHSFKHIVNGFAVHTTPSQASKLRDVEGVRLVEKDRGAKLMTTYTPK 135

Query: 130 FLGVPSKSRLCSKKTKTSPDGEGIVIGFVDSGIDPTHPSFGFDDGY---EDLRLF---CE 189
           FLG+  +  + +++      GEGIVIGFVD+GI+PTHPSF F+       +L  F   CE
Sbjct: 136 FLGL--REGVWAQEGGERNAGEGIVIGFVDTGINPTHPSFAFNPFNPFTSNLSRFSGACE 195

Query: 190 KGRFFPLSSCNGKIVGARFFSAGARAVAKLNSSVDFLSPFDVEGHGSHVASIAAGNGGVP 249
            G  FP SSCNGKIV ARFFSAGA+A   LN+SVDFLSP+D  GHGSHVAS AAGN GVP
Sbjct: 196 AGPHFPASSCNGKIVSARFFSAGAQAATTLNTSVDFLSPYDAVGHGSHVASTAAGNAGVP 255

Query: 250 VYVNGFFYGFASGMAPRARIAVYKAVYPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEP 309
           V VNGFFYG ASGMAPRA+IA+YKAVYPT++TLTDV+SAIDQAV+DGVDIL LSVGP+EP
Sbjct: 256 VVVNGFFYGRASGMAPRAQIAIYKAVYPTVATLTDVISAIDQAVLDGVDILTLSVGPDEP 315

Query: 310 PEEGLTFLSIYDIAILSATRAGILVVQAVGNNGPASATVVSYSPWAVGVAASGTDRVYPA 369
           PE+ +TFL+++DIA+L A RAG+LVVQA GN GP  +TVVS+SPWAVGVAASGTDR YP 
Sbjct: 316 PEDTITFLNLFDIALLFARRAGVLVVQAAGNKGPGPSTVVSFSPWAVGVAASGTDRSYPG 375

Query: 370 SLLLGNGQKVGGVGLSGGSIHFCGYIFWDLYYSSQSNFLNKGFPLYSKHLSSSSGPTFFL 429
            LLLGNG+KVGG+GLSG S+   G     L  +  +   N  FP           P+   
Sbjct: 376 CLLLGNGRKVGGLGLSGPSLG-NGLFLHKLVLARDAARTNGSFP---------ETPSLI- 435

Query: 430 HKLVLAKDAMKQNGTIPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAIIHTAM 489
                             EECQ P+A DP +V+ SIV+C+FS+GF NGTSS+ AII TA 
Sbjct: 436 ------------------EECQSPQALDPRVVRGSIVICTFSEGFSNGTSSVTAIISTAK 495

Query: 490 TLRFIGFALIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDANGMVSEF 549
            L F GF  +ANP+YGDFIAEPI F +PG+LIP+ +D Q ILNYYE +T +  + +V  F
Sbjct: 496 ALGFAGFVFVANPSYGDFIAEPIPFDIPGLLIPSVADVQVILNYYERHTHRGESKLVRRF 555

Query: 550 KGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPL 609
           + KAAIGEGRVASF SQAPTVSRFSSRGPD+++  R   DVLKPDILAPG ++WAAWSP+
Sbjct: 556 RAKAAIGEGRVASFKSQAPTVSRFSSRGPDFIDAKRNPTDVLKPDILAPGQRVWAAWSPI 615

Query: 610 SVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGD 669
           S ++P+L+G  FAL+SGTSMA PHI GIAALIKQ NPSWTPSMI+SA+ TTATKYD +G 
Sbjct: 616 SASDPILEGLSFALVSGTSMATPHIAGIAALIKQYNPSWTPSMISSAIDTTATKYDNHGQ 675

Query: 670 LIQAEGFDIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAE--------YEDYINFLCSLP 729
           +I AEGF I  LYPSTPF+LG+GLV P+ A+DPGLVF +E        Y+DY+ FLCSLP
Sbjct: 676 VIMAEGFSIGSLYPSTPFELGSGLVTPSCAIDPGLVFSSESWGVDEAGYKDYVAFLCSLP 735

Query: 730 G-DPAVIKNTTGGQCNASVPQPHPADLNLPSVTISSLVGHQVLHRRVKNVGTKVETYVWS 789
           G +PA IK   G  CN S+   HPA+LNLPS+TIS+L+G   + R V+NV  + ETY+ +
Sbjct: 736 GMNPATIKIAIGESCNYSL--THPANLNLPSITISALIGSLSVQRTVRNVRNEPETYLCA 795

Query: 790 VIPPNGTTININPPWFTIAPGGLQNLEIQIKVTHKMNHFTFGEILFTGSLNHIGRIPLSV 809
           V+PPNGTT+++NPPWFTIAP G Q L++Q+ VT  MN F+FGEI+ TGSL HI +IPLSV
Sbjct: 796 VLPPNGTTVSLNPPWFTIAPQGTQELDVQLNVTKAMNDFSFGEIILTGSLGHIVKIPLSV 827

BLAST of CmaCh18G005700 vs. TrEMBL
Match: A0A067L7P5_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02112 PE=4 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 8.2e-267
Identity = 498/842 (59.14%), Postives = 593/842 (70.43%), Query Frame = 1

Query: 16  VLVYCFAEERQIYLVLMEES---------------------------------------S 75
           + V CFAEER +YLVLME                                          
Sbjct: 16  ICVACFAEERSVYLVLMEGEPVAFHGGRSPHNYHHHHHHRKRKLGLNSEVSQAHAKRLVD 75

Query: 76  NEDHLLQNTLK--SYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTT 135
           + D LLQ+TL+  SY+KL SFKHI+NGFAVH TPS+  KLR A GVKLVERDRG + MT+
Sbjct: 76  SHDQLLQSTLEIGSYSKLYSFKHIVNGFAVHVTPSQVDKLRVAPGVKLVERDRGAKLMTS 135

Query: 136 YTPKFLGVPSKSRLCSKKTKTSPDGEGIVIGFVDSGIDPTHPSFGFD------DGYEDLR 195
           YTP+FLG+P    + +++      GEGIVIGFVD+GI+P HPSF +D             
Sbjct: 136 YTPQFLGLPQG--VWTQEGGDRNAGEGIVIGFVDTGINPRHPSFAYDPLNPLTSNISHFS 195

Query: 196 LFCEKGRFFPLSSCNGKIVGARFFSAGARAVAKLNSSVDFLSPFDVEGHGSHVASIAAGN 255
             C+ G  FP +SCNGKIV A+FFSAGA+A+A L+ SVD LSPFD  GHGSHVAS AAGN
Sbjct: 196 GTCQTGPRFPANSCNGKIVSAKFFSAGAQAIATLDPSVDILSPFDAVGHGSHVASTAAGN 255

Query: 256 GGVPVYVNGFFYGFASGMAPRARIAVYKAVYPTMSTLTDVVSAIDQAVIDGVDILALSVG 315
            GVPV  NGF+YG ASGMAPRARIAVYKAVYPT+ TLTDVV+ IDQA +DGVDIL LSVG
Sbjct: 256 AGVPVVANGFYYGRASGMAPRARIAVYKAVYPTVGTLTDVVAGIDQATMDGVDILTLSVG 315

Query: 316 PNEPPEEGLTFLSIYDIAILSATRAGILVVQAVGNNGPASATVVSYSPWAVGVAASGTDR 375
           P+EPPE+ LTFLS++D+ +L A RAG+ VVQA GNNGP  +TVVSYSPWAVGVAA  TDR
Sbjct: 316 PDEPPEDTLTFLSVFDVFMLFARRAGVFVVQAAGNNGPDPSTVVSYSPWAVGVAACTTDR 375

Query: 376 VYPASLLLGNGQKVGGVGLSGGSIHFCGYIFWDLYYSSQSNFLNKGFPLYSKHLSSSSGP 435
           +YP SLLLGNGQKVGGVGLS  +    G   + L  +  +   N  FP   +++      
Sbjct: 376 IYPGSLLLGNGQKVGGVGLSAPTFG-DGRFLYKLVLAQDAVKANGTFPRTPQYI------ 435

Query: 436 TFFLHKLVLAKDAMKQNGTIPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAII 495
                                 EECQYPE+ DP++V++SIV+C+FS GF NGTS++ AII
Sbjct: 436 ----------------------EECQYPESLDPSVVRDSIVICTFSTGFYNGTSTINAII 495

Query: 496 HTAMTLRFIGFALIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDANGM 555
            T  TL F+GFAL+ANP YGDFIAEPI F + GI+I N +D+Q IL YYE    +D  G 
Sbjct: 496 DTTRTLGFMGFALVANPIYGDFIAEPIPFAVSGIMISNVADAQIILQYYEQQIKRDERGF 555

Query: 556 VSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAA 615
           V+EF  +AAIGEGRVASF  +AP VSRFSSRGPD++++NRT ADVLKPDILAPGHQIWAA
Sbjct: 556 VTEFHARAAIGEGRVASFEGRAPIVSRFSSRGPDFIDINRTPADVLKPDILAPGHQIWAA 615

Query: 616 WSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYD 675
           WSPLS  EP+L GY+FAL+SGTSMA PHI GIAA IKQ NP WTPSMI+SAMSTTATKYD
Sbjct: 616 WSPLSALEPMLTGYKFALLSGTSMATPHIGGIAAFIKQMNPFWTPSMISSAMSTTATKYD 675

Query: 676 MNGDLIQAEGFDIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAEYEDYINFLCSLPG-DP 735
             G  I AEGFDI+  YPST FDLGAGLV PT ++DPGLVF +E+EDYI+FLCSLP  + 
Sbjct: 676 NYGQPILAEGFDINSFYPSTHFDLGAGLVNPTRSVDPGLVFTSEFEDYISFLCSLPNINR 735

Query: 736 AVIKNTTGGQCNASVPQPHPADLNLPSVTISSLVGHQVLHRRVKNVGTKVETYVWSVIPP 795
             +K  TG  CN      HPA+LNLPSVTIS+L G Q + R VKNVG+K ETY+ SV+ P
Sbjct: 736 TTVKVATGESCNQLF--SHPANLNLPSVTISALKGSQTVQRSVKNVGSKPETYLGSVMSP 795

Query: 796 NGTTININPPWFTIAPGGLQNLEIQIKVTHKMNHFTFGEILFTGSLNHIGRIPLSVLAVS 810
           NGTT+ ++P WFTIAP G Q++EIQ  VT   + F+FGEI+FTGSLNHI RIPLSV  VS
Sbjct: 796 NGTTVTLSPTWFTIAPQGTQHIEIQFLVTKAGDEFSFGEIVFTGSLNHIVRIPLSVFPVS 824

BLAST of CmaCh18G005700 vs. TrEMBL
Match: A0A067GF75_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g003446mg PE=3 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 3.9e-261
Identity = 487/839 (58.05%), Postives = 588/839 (70.08%), Query Frame = 1

Query: 11  LIASLVLVYCFAEERQIYLVLMEES----------------------------SNEDHLL 70
           L+ ++  + CFAEER IYLVL+E                               + D +L
Sbjct: 18  LVLAISFIGCFAEERDIYLVLIEGEPLAFHGSDDKRRFDLNSDAYKGQTKRLMDSHDRIL 77

Query: 71  QNTLK--SYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFL 130
           Q+TL+  SY KL SFK+ +NGFAVH TP++A KL  A  VKLVERDR  + MT+YTP+FL
Sbjct: 78  QSTLEIGSYNKLYSFKYTVNGFAVHLTPTQAKKLENAPQVKLVERDRRAKLMTSYTPQFL 137

Query: 131 GVPSKSRLCSKKTKTSPDGEGIVIGFVDSGIDPTHPSFG----FDDGYEDLRLFCEKGRF 190
           G+P    + +++      GEGIVIGFVD+GI+P+HPSF     F+         CE G  
Sbjct: 138 GLPQG--VWTQRGGDKNAGEGIVIGFVDTGINPSHPSFANYNPFEPNISHFSGDCETGPR 197

Query: 191 FPLSSCNGKIVGARFFSAGARAVAKLNSSVDFLSPFDVEGHGSHVASIAAGNGGVPVYVN 250
           FPLSSCNGKIV ARFFSAGA+AVA LN+SVDFLSPFD  GHGSHVAS AAGN GVPV V+
Sbjct: 198 FPLSSCNGKIVSARFFSAGAQAVATLNTSVDFLSPFDAVGHGSHVASTAAGNAGVPVVVD 257

Query: 251 GFFYGFASGMAPRARIAVYKAVYPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEG 310
           GFFYG ASGMAP ARIAVYKA+YPT+ TL DV++AIDQA +DGVDIL LS+GP+EPP + 
Sbjct: 258 GFFYGLASGMAPCARIAVYKAMYPTVGTLADVIAAIDQATMDGVDILTLSIGPDEPPRDT 317

Query: 311 LTFLSIYDIAILSATRAGILVVQAVGNNGPASATVVSYSPWAVGVAASGTDRVYPASLLL 370
           +T L I+D+ +L A RAG+ VVQA GN GPA +TVVSYSPWAV  AA  TDR+YP SLLL
Sbjct: 318 ITMLGIFDVLMLFARRAGVFVVQAAGNQGPAPSTVVSYSPWAVAAAACTTDRIYPGSLLL 377

Query: 371 GNGQKVGGVGLSGGSIHFCGYIFWDLYYSSQSNFLNKGFPLYSKHLSSSSGPTFFLHKLV 430
           GNG K+GGVGLSG +                      G PL             FL KLV
Sbjct: 378 GNGLKLGGVGLSGPTC---------------------GRPL-------------FLSKLV 437

Query: 431 LAKDA-MKQNGTIPS-----EECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAIIHT 490
           LA+D  ++ NGT P      EECQYPEAF+P+LVQ S+V+C+FS GF N TS+L A+I+T
Sbjct: 438 LARDVILRVNGTFPRTPQYIEECQYPEAFEPSLVQGSVVICTFSDGFYNQTSTLTAVINT 497

Query: 491 AMTLRFIGFALIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDANGMVS 550
           A+TL F+GF LIAN +YGDF+AEPI F +PGILIP  S S+ IL YYE  T +D  G+  
Sbjct: 498 AITLGFMGFILIANSHYGDFVAEPIPFAVPGILIPKVSTSEIILQYYEQQTHRDERGVAI 557

Query: 551 EFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWS 610
           +F  +A IGEGRVASF  +AP VSRFSSRGPD+ +++R   DVLKPD++APGHQIWAAWS
Sbjct: 558 KFNAQAGIGEGRVASFEGRAPIVSRFSSRGPDFTDLSRNPTDVLKPDVIAPGHQIWAAWS 617

Query: 611 PLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMN 670
           P+S  +P+L G  FAL+SGTSMA PHI GIAALIKQ NPSWTP+MIASA+S+TATKYD  
Sbjct: 618 PVSALDPMLTGCNFALLSGTSMATPHIAGIAALIKQHNPSWTPTMIASAISSTATKYDNY 677

Query: 671 GDLIQAEGFDIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAEYEDYINFLCSL-PGDPAV 730
           G LI AEGF+I   Y ST FD G+GLV  T ALDPGLV   E+EDYI+FLCSL   DP  
Sbjct: 678 GQLIMAEGFEITSTYNSTHFDFGSGLVSATRALDPGLVLSVEFEDYISFLCSLADSDPVS 737

Query: 731 IKNTTGGQCNASVPQPHPADLNLPSVTISSLVGHQVLHRRVKNVGTKVETYVWSVIPPNG 790
           IK  TG  CN S+   HPA+LNLPSVT+S++    +L R +KNVG K ETY+ SV+ PNG
Sbjct: 738 IKAATGIWCNHSL--SHPANLNLPSVTVSAVAKSLILQRSLKNVGNKTETYLTSVVHPNG 797

Query: 791 TTININPPWFTIAPGGLQNLEIQIKVTHKMNHFTFGEILFTGSLNHIGRIPLSVLAVSV 809
           TT+++ PPWFTIAP G Q+L IQ  VT  +  F+FGEI+ TGSLNHI RIPLSV  VS+
Sbjct: 798 TTVSLYPPWFTIAPQGTQDLAIQFNVTQAIGDFSFGEIVLTGSLNHIVRIPLSVKPVSI 818

BLAST of CmaCh18G005700 vs. TrEMBL
Match: A0A068U8B7_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00017837001 PE=3 SV=1)

HSP 1 Score: 905.6 bits (2339), Expect = 4.4e-260
Identity = 483/844 (57.23%), Postives = 590/844 (69.91%), Query Frame = 1

Query: 11  LIASLVLVYCFAEERQIYLVLME---------------------------ESS------- 70
           L+  ++ + C AEER IY+VLME                           E+S       
Sbjct: 17  LLTFVIAISCIAEERDIYIVLMEGEPVVFHHQKNSGTMPTEKGEGSDPNSEASKSHAKHL 76

Query: 71  --NEDHLLQNTLK--SYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKM 130
             + D LLQ  L   SYTKL SFK+I+NGFAVHT+PS+  ++++A GVK+VERDR  + M
Sbjct: 77  IDSHDQLLQTALDAGSYTKLYSFKNIVNGFAVHTSPSQVERIKKAPGVKIVERDRRAKLM 136

Query: 131 TTYTPKFLGVPSKSRLCSKKTKTSPDGEGIVIGFVDSGIDPTHPSFGFDDG---YEDLRL 190
           TTYTP+FLG+P+   + +++      GEG VIGFVDSGIDP HPSF  D       +L  
Sbjct: 137 TTYTPQFLGLPA---VWTQEGGDRNAGEGTVIGFVDSGIDPLHPSFAGDPTNPYISELPR 196

Query: 191 F---CEKGRFFPLSSCNGKIVGARFFSAGARAVAKLNSSVDFLSPFDVEGHGSHVASIAA 250
           F   CE G  FP +SCNGKIV ARFFSAGA+A   LN+S+DFLSPFD  GHGSHVASIAA
Sbjct: 197 FSGTCESGPLFPETSCNGKIVSARFFSAGAQAATALNASMDFLSPFDAAGHGSHVASIAA 256

Query: 251 GNGGVPVYVNGFFYGFASGMAPRARIAVYKAVYPTMSTLTDVVSAIDQAVIDGVDILALS 310
           GN GVPV V+GF+YG ASGMAPRARIAVYKA+YP++  LTDV++AIDQAV+DGVDIL LS
Sbjct: 257 GNFGVPVVVDGFYYGKASGMAPRARIAVYKAIYPSVGVLTDVLAAIDQAVLDGVDILTLS 316

Query: 311 VGPNEPPEEGLTFLSIYDIAILSATRAGILVVQAVGNNGPASATVVSYSPWAVGVAASGT 370
           +GP++PPE+ +TFL  ++I +L+A +AGILV+QAVGN GP   TVVSYSPW VGVAAS T
Sbjct: 317 IGPDQPPEDAITFLGTFEIFMLAANKAGILVIQAVGNGGPGPYTVVSYSPWTVGVAASDT 376

Query: 371 DRVYPASLLLGNGQKVGGVGLSGGSIHFCGYIFWDLYYSSQSNFLNKGFPLYSKHLSSSS 430
           DR YP +L+LGNGQK+GGVGLSG S    G +   L  +  +   N  FP  S+++    
Sbjct: 377 DRSYPGTLILGNGQKIGGVGLSGPS-SGQGLLQHRLILAKDAVIRNGNFPRISQYI---- 436

Query: 431 GPTFFLHKLVLAKDAMKQNGTIPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAA 490
                                   EECQYPEA DP +V  S+V+C+FS GF NGTS+L A
Sbjct: 437 ------------------------EECQYPEALDPAVVLGSVVICTFSDGFYNGTSNLTA 496

Query: 491 IIHTAMTLRFIGFALIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDAN 550
           II TA  L F+GF L+ANP YGDFIAEPI F +PGI+IP  SD+  I  YYE  T +D  
Sbjct: 497 IIKTAKVLGFVGFVLVANPRYGDFIAEPIPFSVPGIMIPRTSDALLISQYYEEQTSRDKR 556

Query: 551 GMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIW 610
           G+V  + G+AAIGEGR A+   +APTVSRFS+RGPDY++  +   DVLKPDILAPGHQIW
Sbjct: 557 GLVISYSGRAAIGEGRTAACMGRAPTVSRFSARGPDYIDQRKNPTDVLKPDILAPGHQIW 616

Query: 611 AAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATK 670
           AAWSPLS ++P+L G  FAL+SGTSMA PHI GIAALIKQ NP WTPSMIASAMSTTATK
Sbjct: 617 AAWSPLSASDPMLAGQNFALISGTSMATPHISGIAALIKQNNPKWTPSMIASAMSTTATK 676

Query: 671 YDMNGDLIQAEGFDIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAEYEDYINFLCSLPG- 730
           YD  GD I A GF+I  LY + PF  GAG V P+ A+DPGLVF A YEDYI+FLCSLP  
Sbjct: 677 YDNQGDQIMAHGFEIDSLYTAAPFGFGAGHVNPSRAMDPGLVFSAGYEDYISFLCSLPNL 736

Query: 731 DPAVIKNTTGGQCNASVPQPHPADLNLPSVTISSLVGHQVLHRRVKNVGTKVETYVWSVI 790
           DPA I+  TGG C +S     PADLNL SVTI++L G + +HRRV NV +K ETYV  V+
Sbjct: 737 DPASIRTATGGPCTSSFGS--PADLNLASVTITALSGSRTVHRRVTNVASKPETYVCGVL 796

Query: 791 PPNGTTININPPWFTIAPGGLQNLEIQIKVTHKMNHFTFGEILFTGSLNHIGRIPLSVLA 810
           PP G  I+I+PPWF +AP G+Q+LEI++ VT  ++ F+FGEI+ TGSL+HI RIPLSVL 
Sbjct: 797 PPVGVMIDIHPPWFRVAPQGIQDLEIRLNVTQALDDFSFGEIVLTGSLDHIARIPLSVLP 826

BLAST of CmaCh18G005700 vs. TAIR10
Match: AT1G62340.1 (AT1G62340.1 PA-domain containing subtilase family protein)

HSP 1 Score: 801.2 bits (2068), Expect = 5.9e-232
Identity = 426/780 (54.62%), Postives = 527/780 (67.56%), Query Frame = 1

Query: 39  DHLLQNTLK--SYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYT 98
           D +L +TL+  SYTKL SFKH++N  AV TT S+A KL +  GVK VE D+GV+ MTTYT
Sbjct: 83  DEILGSTLEKGSYTKLYSFKHVINAIAVRTTASQAKKLGKTKGVKAVEEDKGVKLMTTYT 142

Query: 99  PKFLGVPSKSRLCSKKTKTSPDGEGIVIGFVDSGIDPTHPSFG-------FDDGYEDLRL 158
           P FL +P +             GE IVIGFVD+GI+PTHPSF        +      L  
Sbjct: 143 PDFLELPQQVWQKISNEGDRRAGEDIVIGFVDTGINPTHPSFAALDLTNPYSSNLSRLHF 202

Query: 159 F--CEKGRFFPLSSCNGKIVGARFFSAGARAVAKLNSSVDFLSPFDVEGHGSHVASIAAG 218
              CE G FFP  SCNGKI+ ARFFSAGARA   LNSS+D LSPFD  GHGSHVASIAAG
Sbjct: 203 SGDCEIGPFFPPGSCNGKIISARFFSAGARASGALNSSLDILSPFDASGHGSHVASIAAG 262

Query: 219 NGGVPVYVNGFFYGFASGMAPRARIAVYKAVYPTMSTLTDVVSAIDQAVIDGVDILALSV 278
           N GVPV V+GFFYG ASGMAPR+RIAVYKA+YP++ TL DV++AIDQA++DGVD+L LSV
Sbjct: 263 NAGVPVIVDGFFYGRASGMAPRSRIAVYKAIYPSIGTLVDVIAAIDQAIMDGVDVLTLSV 322

Query: 279 GPNEPPEEGLTFLSIYDIAILSATRAGILVVQAVGNNGPASATVVSYSPWAVGVAASGTD 338
           GP+EPP +  T L I+D+A+L A +AG+ VVQAVGNNGP+ ++V+SYSPW VGVAA  TD
Sbjct: 323 GPDEPPVDKPTVLGIFDLAMLLARKAGVFVVQAVGNNGPSPSSVLSYSPWVVGVAAGNTD 382

Query: 339 RVYPASLLLGNGQKVGGVGLSGGSIHFCGYIFWDLYYSSQSNFLNKGFPLYSKHLSSSSG 398
           R YPA L+L  GQ V GVGLSG ++                     G PL    L  +  
Sbjct: 383 RSYPAPLILDGGQTVQGVGLSGPTL---------------------GAPLVQHRLVLAKD 442

Query: 399 PTFFLHKLVLAKDAMKQNGTIPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAI 458
                  ++       +    P         FDP  V  SIV+C+FS GF N  S++ AI
Sbjct: 443 AVRTNGSVLQPLTRDIEECQRPEN-------FDPAAVFGSIVICTFSDGFYNQMSTVLAI 502

Query: 459 IHTAMTLRFIGFALIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDANG 518
             TA TL F+GF LIANP +GD++AEP+ F  PGILIP  S +Q IL YYE  T +D  G
Sbjct: 503 TQTARTLGFMGFILIANPRFGDYVAEPVIFSAPGILIPTVSAAQIILRYYEEKTFRDTRG 562

Query: 519 MVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWA 578
           + ++F  +A IGEGR + F  +AP VSRFSSRGP +++  R+  DVLKPDILAPGHQIW 
Sbjct: 563 VATQFGARARIGEGRNSVFAGKAPVVSRFSSRGPAFIDATRSPLDVLKPDILAPGHQIWG 622

Query: 579 AWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKY 638
           AWS  S  +P+L G  FA++SGTSMA PHI GI ALIKQ NPSWTP+MIASA+STTA +Y
Sbjct: 623 AWSLPSAFDPILTGRSFAILSGTSMATPHIAGIGALIKQLNPSWTPAMIASAISTTANEY 682

Query: 639 DMNGDLIQAEGFDIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAEYEDYINFLCSLPG-D 698
           D NG++I AE +++ RL+PS  FD GAG V P  ALDPGLV PA +EDYI+FLCSLP   
Sbjct: 683 DSNGEIISAEYYELSRLFPSNHFDHGAGHVNPARALDPGLVLPAGFEDYISFLCSLPNIS 742

Query: 699 PAVIKNTTGGQCNASVPQPHPADLNLPSVTISSLVGHQVLHRRVKNVGTKVETYVWSVIP 758
           PA I++ TG  C  ++   HPA+LN PSVTIS+L    V+ R  ++V  K ETY+ SV+P
Sbjct: 743 PATIRDATGVLCTTTL--SHPANLNHPSVTISALKESLVVRRSFQDVSNKTETYLGSVLP 802

Query: 759 PNGTTININPPWFTIAPGGLQNLEIQIKVTHKMNHFTFGEILFTGSLNHIGRIPLSVLAV 807
           PNGTT+ + P WFT+ P   Q+L+I+  VT  +N FTFGE++ TGSLNHI RIPLSV  +
Sbjct: 803 PNGTTVRLTPTWFTVPPQKTQDLDIEFNVTQVLNKFTFGEVVLTGSLNHIIRIPLSVKTI 832

BLAST of CmaCh18G005700 vs. TAIR10
Match: AT2G19170.1 (AT2G19170.1 subtilisin-like serine protease 3)

HSP 1 Score: 580.1 bits (1494), Expect = 2.1e-165
Identity = 337/768 (43.88%), Postives = 456/768 (59.38%), Query Frame = 1

Query: 48  SYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSR 107
           SY KL S+KH++NGFA H +P +A  LR+A GV+ V++D  VR++TT+TP+FLG+P+   
Sbjct: 82  SYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRLTTHTPEFLGLPTD-- 141

Query: 108 LCSKKTKTSPDGEGIVIGFVDSGIDPTHPSFGFDDG--YEDLRLF---CEKGRFFPLSSC 167
           +          GE IVIGFVDSGI P HPSF       Y  L  +   CE+      S C
Sbjct: 142 VWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLPHYKGKCEEDPHTKKSFC 201

Query: 168 NGKIVGARFFSAGARAVAKLNSSVDFLSPFDVEGHGSHVASIAAGNGGVPVYVNGFFYGF 227
           N KIVGA+ F+  A+A    N  +D+ SP D +GHGSH A+IAAGN G+P+ ++G+ +G 
Sbjct: 202 NRKIVGAQHFAEAAKAAGAFNPDIDYASPMDGDGHGSHTAAIAAGNNGIPLRMHGYEFGK 261

Query: 228 ASGMAPRARIAVYKAVYPTMST-LTDVVSAIDQAVIDGVDILALSVGPNEPPEEG-LTFL 287
           ASGMAPRARIAVYKA+Y      + DVV+AIDQAV DGVDIL+LSVGPN PP     TFL
Sbjct: 262 ASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPTTTKTTFL 321

Query: 288 SIYDIAILSATRAGILVVQAVGNNGPASATVVSYSPWAVGVAASGTDRVYPASLLLGNGQ 347
           + +D  +L A +AG+ V QA GN GP   T+VSYSPW   VAA+  DR Y   L LGNG+
Sbjct: 322 NPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDDRRYKNHLTLGNGK 381

Query: 348 KVGGVGLSGGSIHFCGYIFWDLYYSSQSNFLNKGFPLYSKHLSSSSGPTFFLHKLVLAKD 407
            + G+GLS  +                     +   LY+  L S+       + ++L   
Sbjct: 382 MLAGMGLSPPT---------------------RPHRLYT--LVSA-------NDVLLDSS 441

Query: 408 AMKQNGTIPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAIIHTAMTLRFIGFA 467
             K N +    +CQ PE F+  LV+ +I+LC +S  F+ GT+S+  ++ TA  L   GF 
Sbjct: 442 VSKYNPS----DCQRPEVFNKKLVEGNILLCGYSFNFVVGTASIKKVVATAKHLGAAGFV 501

Query: 468 LIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDANGMVSEFKGKAAIGE 527
           L+          +P+   +PGILI + S S  +++YY A+T +D  G V  FK + +IG+
Sbjct: 502 LVVENVSPGTKFDPVPSAIPGILITDVSKSMDLIDYYNASTSRDWTGRVKSFKAEGSIGD 561

Query: 528 GRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTEPLLK 587
           G        AP V+ FS+RGP+  + +   AD+LKPDILAPG+ IWAAW P    EP   
Sbjct: 562 GLAPVLHKSAPQVALFSARGPNTKDFSFQDADLLKPDILAPGYLIWAAWCPNGTDEPNYV 621

Query: 588 GYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQAEGF- 647
           G  FAL+SGTSMAAPHI GIAAL+KQK+P W+P+ I SA+ TT+T  D  G L+QA+ + 
Sbjct: 622 GEGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVIDRAGRLLQAQQYS 681

Query: 648 --DIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAEYEDYINFLCSLPGDPA-VIKNTTGG 707
             +   L  +TPFD G+G V P+ ALDPGL+F A YEDY+ FLC+ PG  A  I+N T  
Sbjct: 682 DTEAVTLVKATPFDYGSGHVNPSAALDPGLIFDAGYEDYLGFLCTTPGISAHEIRNYTNT 741

Query: 708 QCNASVPQPHPADLNLPSVTISSLVGHQVLHRRVKNVGTKVETYVWSVIPPNGTTININP 767
            CN  +   HP++ N PS+ +S LVG Q + R+V NV    ETY  +        I +NP
Sbjct: 742 ACNYDM--KHPSNFNAPSIAVSHLVGTQTVTRKVTNVAEVEETYTITARMQPSIAIEVNP 801

Query: 768 PWFTIAPGGLQNLEIQIKVTHKMNHFTFGEILFTGSLNHIGRIPLSVL 805
           P  T+ PG  +   + + V      ++FGE+   GS  H  RIP+  L
Sbjct: 802 PAMTLRPGATRTFSVTMTVRSVSGVYSFGEVKLKGSRGHKVRIPVVAL 811

BLAST of CmaCh18G005700 vs. TAIR10
Match: AT4G30020.1 (AT4G30020.1 PA-domain containing subtilase family protein)

HSP 1 Score: 560.5 bits (1443), Expect = 1.7e-159
Identity = 331/773 (42.82%), Postives = 448/773 (57.96%), Query Frame = 1

Query: 48  SYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSR 107
           SY KL S+KH++NGFA H +P +A  LR+A GVK V+RD  VRK+TT+TP+FLG+P+   
Sbjct: 82  SYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRDWKVRKLTTHTPQFLGLPTD-- 141

Query: 108 LCSKKTKTSPDGEGIVIGFVDSGIDPTHPSFGFDDG------YEDLRLFCEKGRFFPLSS 167
           +          GE IVIGF+DSGI P HPSF           +   +  CE+     +S 
Sbjct: 142 VWPTGGGYDRAGEDIVIGFIDSGIFPHHPSFASHHTTVPYGPHPSYKGKCEEDPHTKISF 201

Query: 168 CNGKIVGARFFSAGARAVAKLNSSVDFLSPFDVEGHGSHVASIAAGNGGVPVYVNGFFYG 227
           CNGKI+GA+ F+  A+A    N  +DF SP D +GHGSH A+IAAGN G+PV ++G+ +G
Sbjct: 202 CNGKIIGAQHFAEAAKAAGAFNPDIDFASPMDGDGHGSHTAAIAAGNNGIPVRMHGYEFG 261

Query: 228 FASGMAPRARIAVYKAVYPTMST-LTDVVSAIDQAVIDGVDILALSVGPNEPPEEG-LTF 287
            ASGMAPRARIAVYKA+Y      + DVV+AIDQAV DGVDIL+LSVGPN PP     TF
Sbjct: 262 KASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPATTKTTF 321

Query: 288 LSIYDIAILSATRAGILVVQAVGNNGPASATVVSYSPWAVGVAASGTDRVYPASLLLGNG 347
           L+ +D  +L A +AG+ V QA GN GP   T+VSYSPW   VAA+  DR Y   L LGNG
Sbjct: 322 LNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDDRRYKNHLTLGNG 381

Query: 348 QKVGGVGLSGGSIHFCGYIFWDLYYSSQSNFLNKGFPLYSKH--LSSSSGPTFFLHKLVL 407
           + + G+GL                  S S   ++ + + S +  L  SSG  +       
Sbjct: 382 KMLAGIGL------------------SPSTRPHRSYKMVSANDVLLGSSGMKY------N 441

Query: 408 AKDAMKQNGTIPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAIIHTAMTLRFI 467
             D  K            PE  +  LV+ +I+LC +S  F+ G++S+  +  TA  L   
Sbjct: 442 PSDCQK------------PEVLNKKLVEGNILLCGYSFNFVAGSASIKKVAETAKHLGAA 501

Query: 468 GFALIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDANGMVSEFKGKAA 527
           GF L+          +P+   +PGILI + S S  +++YY   T +D  G V +FK + +
Sbjct: 502 GFVLVVENVSPGTKFDPVPSCIPGILITDVSKSMDLIDYYNVTTSRDWMGRVKDFKAEGS 561

Query: 528 IGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTEP 587
           IG+G        AP V+ FS+RGP+  + +   AD+LKPDILAPG  IW+AWS     E 
Sbjct: 562 IGDGLEPILHKSAPEVALFSARGPNTKDFSFQDADLLKPDILAPGSLIWSAWSANGTDEA 621

Query: 588 LLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQAE 647
              G  FAL+SGTSMAAPHI GIAAL+KQK+P W+P+ I SA+ TT+T  D  G  +QA+
Sbjct: 622 NYIGEGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVIDRAGRPLQAQ 681

Query: 648 GF---DIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAEYEDYINFLCSLPG-DPAVIKNT 707
            +   +   L  +TPFD G+G V P+ ALDPGL+F A YEDYI FLC+ PG D   IKN 
Sbjct: 682 QYSETETVTLVKATPFDYGSGHVNPSAALDPGLIFDAGYEDYIGFLCTTPGIDAHEIKNF 741

Query: 708 TGGQCNASVPQPHPADLNLPSVTISSLVGHQVLHRRVKNVGTKVETYVWSVIPPNGTTIN 767
           T   CN  +   HP++ N PS+ IS LV  Q + RRV NV  + ETY  +        I 
Sbjct: 742 TNTPCNFKM--VHPSNFNTPSIAISHLVRTQTVTRRVTNVAEEEETYTITSRMEPAIAIE 801

Query: 768 INPPWFTIAPGGLQNLEIQIKVTHKMNHFTFGEILFTGSLNHIGRIPLSVLAV 807
           ++PP  T+  G  +   + + V      ++FG++   GS  H  ++ L V+A+
Sbjct: 802 VSPPAMTVRAGASRTFSVTLTVRSVTGAYSFGQVTLKGSRGH--KVTLPVVAM 812

BLAST of CmaCh18G005700 vs. TAIR10
Match: AT1G30600.1 (AT1G30600.1 Subtilase family protein)

HSP 1 Score: 508.8 bits (1309), Expect = 6.0e-144
Identity = 315/801 (39.33%), Postives = 449/801 (56.05%), Query Frame = 1

Query: 22  AEERQIYLVLMEESSN---EDHLLQNTLK--SYTKLQSFKHIMNGFAVHTTPSEAAKLRQ 81
           A   QIY  L   +S     D LL+N L+  +Y KL S+ +++NGF+   T  +A +L  
Sbjct: 63  ATSSQIYRTLNRSASIIRVHDSLLRNVLRKENYLKLYSYHYLINGFSAVLTRKQADRLAA 122

Query: 82  ANGVKLVERDRGVRKMTTYTPKFLGVPSKSRLCSKKTKTSPDGEGIVIGFVDSGIDPTHP 141
              V+ V  D  V K TT+TP+FLG+P  + L    ++ +  GEG+VIGF+D+GIDPTHP
Sbjct: 123 REEVENVVLDFLVEKATTHTPQFLGLPRGAWLRDGGSEYA--GEGVVIGFIDTGIDPTHP 182

Query: 142 SFGFDDGYEDLRL------FCEKGRFFPLSSCNGKIVGARFFSAGARAVAKLNSSVDFLS 201
           SF          +       CE    FP  SCN K++GAR F+  A +   LNSS D  S
Sbjct: 183 SFSDKISGHTYSVPPHFTGVCEVTIGFPPGSCNRKLIGARHFAESALSRGVLNSSQDDAS 242

Query: 202 PFDVEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAVYPTMSTL-TDVV 261
           PFD EGHG+H AS+AAGN G+PV V G   G ASGMAPRA IA+YKA+Y        D++
Sbjct: 243 PFDGEGHGTHTASVAAGNHGIPVVVAGHRLGNASGMAPRAHIAIYKALYKRFGGFAADII 302

Query: 262 SAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVVQAVGNNGPASA 321
           +AIDQA  DGVDI+ LS+ PN  P    TF +  D+A+LSA +AGI VVQA GN GPA  
Sbjct: 303 AAIDQAAQDGVDIINLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPK 362

Query: 322 TVVSYSPWAVGVAASGTDRVYPASLLLGNGQKVGGVGLSGGSIHFCGYIFWDLYYSSQSN 381
           ++ S+SPW   V A+  DRVY  S++LGN   + GVGL+ G+      I   L  ++ + 
Sbjct: 363 SMSSFSPWIFTVGATSHDRVYSNSIILGNNVTIPGVGLASGT-----RIMHKLVLATHA- 422

Query: 382 FLNKGFPLYSKHLSSSSGPTFFLHKLVLAKDAMKQNGTIPSEECQYPEAFDPNLVQNSIV 441
             N    + + ++      + F  KLV                            Q  I+
Sbjct: 423 LRNGTTVMDAIYVGECQDSSSFDQKLV----------------------------QGKIL 482

Query: 442 LCSFSQGFLNGTSSLAAIIHTAMTLRFIGFALIANPNYGDFIAEPITFRLPGILIPNASD 501
           +CS++  F+ G S++   + TA  L   G     +P+   F        +PGILI +  D
Sbjct: 483 VCSYTVRFILGVSTIKQALLTAKNLTAAGLVFYIDPSATGFQMTSSPMDIPGILISSPQD 542

Query: 502 SQAILNYYEANTCKD-ANGMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNR 561
           SQA+L YY ++  ++  +G +      A I  G   ++G  AP V  FS+RGPD  + + 
Sbjct: 543 SQALLRYYNSSLLRENGSGKIVGSASVAKIVGGMRPTYGITAPKVMYFSARGPDPEDDSF 602

Query: 562 TVADVLKPDILAPGHQIWAAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKN 621
             AD++KP+++APG+ IW AWSPL +     +G +FA+ SGTSM+APH+ GIAALIKQK 
Sbjct: 603 VDADIMKPNLVAPGNAIWGAWSPLGIGTNDFQGERFAMESGTSMSAPHVTGIAALIKQKF 662

Query: 622 PSWTPSMIASAMSTTATKYDMNGDLIQAEGFDIH---RLYPSTPFDLGAGLVKPTNALDP 681
           P +TP+ IASA+STTA+  D  G+ I A+   ++      P+TPFD+G+G V  T ALDP
Sbjct: 663 PHFTPAAIASALSTTASLSDRKGEHIMAQRTVLNPDISQSPATPFDMGSGFVNATAALDP 722

Query: 682 GLVFPAEYEDYINFLCSLPGDPAVIKNTTGGQCNASVPQPHPADLNLPSVTISSLVGHQV 741
           GL+F   Y +Y+ FLC + G   V+ N TG  C++       +DLNLPSVTI+ LVG + 
Sbjct: 723 GLIFDIGYNEYMKFLCGINGSSPVVLNYTGESCSSYNSSLAASDLNLPSVTIAKLVGTRA 782

Query: 742 LHRRVKNVGTKV--ETYVWSVIPPNGTTININPPWFTIAPGGLQNLEIQIKVTHKMNHFT 801
           + R V N+ T    ETY+   + P+  ++ ++P  FTI  G  + L +  +    ++  +
Sbjct: 783 VLRWVTNIATTATNETYIVGWMAPDSVSVKVSPAKFTIGNGQTRVLSLVFRAMKNVSMAS 827

Query: 802 FGEILFTGSLNHIGRIPLSVL 805
           FG I   G   H+  IP++V+
Sbjct: 843 FGRIGLFGDRGHVVNIPVAVI 827

BLAST of CmaCh18G005700 vs. TAIR10
Match: AT5G44530.1 (AT5G44530.1 Subtilase family protein)

HSP 1 Score: 300.8 bits (769), Expect = 2.5e-81
Identity = 167/422 (39.57%), Postives = 243/422 (57.58%), Query Frame = 1

Query: 392 LHKLVLAKDAMKQNGTIPSE----ECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAI 451
           ++K++ A  A+  + ++  +    ECQ  E FD + V   +++CS+S  F+ G S++   
Sbjct: 415 MYKMISAFHALNNSTSVDKDMYVGECQDYENFDQDRVSGKLLICSYSARFVLGLSTIKQA 474

Query: 452 IHTAMTLRFIGFALIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDANG 511
           +  A  L   G     +P    F   P    +PGI+IP+  DS+ +L YY ++  +D   
Sbjct: 475 LDVAKNLSATGVIFYIDPYVLGFEINPTPMDMPGIIIPSVEDSKTLLKYYNSSIQRDVTT 534

Query: 512 M-VSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIW 571
             +  F   AAI  G  A+F ++AP V  +S+RGPD  + +   ADVLKP+++APG+ IW
Sbjct: 535 KEIVSFGAVAAIEGGLNANFSNRAPKVMYYSARGPDPEDNSFNDADVLKPNLVAPGNSIW 594

Query: 572 AAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATK 631
            AWS  S      +G +FA+MSGTSMAAPH+ G+AALIKQ  P +TPS I+SA+STTA  
Sbjct: 595 GAWSSASTDSTEFEGEKFAMMSGTSMAAPHVAGVAALIKQSYPQFTPSTISSALSTTALL 654

Query: 632 YDMNGDLIQAE---GFDIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAEYEDYINFLCSL 691
            D  G  I A+         LY +TP D+G+G V  T ALDPGLVF   +EDYI+FLC +
Sbjct: 655 NDNKGSPIMAQRTYSNPDQSLYTATPSDMGSGFVNATAALDPGLVFDTSFEDYISFLCGI 714

Query: 692 PGDPAVIKNTTGGQCNASVPQPHPADLNLPSVTISSLVGHQVLHRRVKNVGTKVETYVWS 751
            G   V+ N TG +C A+       DLNLPS+T+S+L G Q   R ++N+    ETY   
Sbjct: 715 NGSDTVVFNYTGFRCPANNTPVSGFDLNLPSITVSTLSGTQTFQRSMRNIAGN-ETYNVG 774

Query: 752 VIPPNGTTININPPWFTIAPGGLQNLEIQIKVTHKMNHFTFGEILFTGSLNHIGRIPLSV 806
             PP G ++ ++P  F+IA G  Q L + + VT   +  +FG I   G+  HI  IP++V
Sbjct: 775 WSPPYGVSMKVSPTQFSIAMGENQVLSVTLTVTKNSSSSSFGRIGLFGNTGHIVNIPVTV 834

BLAST of CmaCh18G005700 vs. NCBI nr
Match: gi|659118464|ref|XP_008459133.1| (PREDICTED: LOW QUALITY PROTEIN: cucumisin, partial [Cucumis melo])

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 584/815 (71.66%), Postives = 653/815 (80.12%), Query Frame = 1

Query: 17  LVYCFAEERQIYLVLMEE-------------SSNEDH--LLQNTLKSYTKLQSFKHIMNG 76
           LV C+AE+R+I+LV+ME+             S  + H  +L+N++K+Y KL SFK I+NG
Sbjct: 10  LVSCYAEKREIFLVVMEDDGGYELRNSKEMLSIEDSHKKVLENSIKNYRKLHSFKKILNG 69

Query: 77  FAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRLCSKKTK---TSPD 136
           FAVHTTPSEA+KLR+ANGVKLVE DRGV+KMTTYTP+FLG+   +           +   
Sbjct: 70  FAVHTTPSEASKLREANGVKLVELDRGVKKMTTYTPEFLGLVKNNNDYKYNYSGGISGAG 129

Query: 137 GEGIVIGFVDSGIDPTHPSFGFDDGYEDL---RLFCEKGRFFPLSSCNGKIVGARFFSAG 196
           G+GI+IGFVDSGI P HPSF  + G +D     L CE+G  FP   CNGKIV A FFSAG
Sbjct: 130 GDGILIGFVDSGIYPKHPSFSNNFGKDDDDYGELVCEEGPLFPKGCCNGKIVSAMFFSAG 189

Query: 197 ARAVAKLNSSVDFLSPFDVEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVY 256
           A+A A LNSS+DFLSPFD EGHGSHVASIAAGN  VPV V+GFFYG ASG+AP ARI VY
Sbjct: 190 AQAAAVLNSSIDFLSPFDAEGHGSHVASIAAGNAEVPVIVDGFFYGLASGIAPHARIGVY 249

Query: 257 KAVYPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGI 316
           KAVYPT++TLTDV+SAIDQAVIDGVDILALSVGPNEPPE G TFLSIYDIAILSATRAGI
Sbjct: 250 KAVYPTIATLTDVISAIDQAVIDGVDILALSVGPNEPPEVGFTFLSIYDIAILSATRAGI 309

Query: 317 LVVQAVGNNGPASATVVSYSPWAVGVAASGTDRVYPASLLLGNGQKVGGVGLSGGSIHFC 376
           LVVQA GNNGPA ATVVSYSPWA+GVAASGTDRVY +SLLLGNGQK+GGVG+SG S+   
Sbjct: 310 LVVQAAGNNGPARATVVSYSPWAIGVAASGTDRVYSSSLLLGNGQKIGGVGMSGPSL--- 369

Query: 377 GYIFWDLYYSSQSNFLNKGFPLYSKHLSSSSGPTFFLHKLVLAKDAMKQNGTIPS--EEC 436
                         FL+K        + + +                 Q   IPS  EEC
Sbjct: 370 ----------GSEFFLHKLVLAKDAKMQNET-----------------QYDDIPSYIEEC 429

Query: 437 QYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAIIHTAMTLRFIGFALIANPNYGDFIAE 496
           Q PEAFDPN+VQNSIVLCSFSQGFLNGTSSLAAIIHTA  L+F+GF LIANPNYGDFIAE
Sbjct: 430 QNPEAFDPNIVQNSIVLCSFSQGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFIAE 489

Query: 497 PITFRLPGILIPNASDSQAILNYYEANTCKDANGMVSEFKGKAAIGEGRVASFGSQAPTV 556
           PI FR+PGIL+P+ SD+Q IL YYE NTCKD  GMV EFKGKA IGEGR+ASFG +APTV
Sbjct: 490 PIPFRVPGILVPSVSDTQVILKYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAPTV 549

Query: 557 SRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTEPLLKGYQFALMSGTSMA 616
           SRFSSRGPDY+N+NR++ADVLKPDILAPGHQIWAAWSPLS +EPLLKGY+FALMSGTSMA
Sbjct: 550 SRFSSRGPDYININRSLADVLKPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTSMA 609

Query: 617 APHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQAEGFDIHRLYPSTPFDLG 676
           APHIVG+AALIKQK PSWTPSMIASAMSTTATKYDMNGDLIQAEGF++H LYPSTPFD G
Sbjct: 610 APHIVGVAALIKQKYPSWTPSMIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFG 669

Query: 677 AGLVKPTNALDPGLVFPAEYEDYINFLCSLPG-DPAVIKNTTGGQCNASVPQPHPADLNL 736
           AGLV PTNALDPGLVFP EYEDYINFLCSLPG DPAV+K+ TGGQCNAS+   HPADLNL
Sbjct: 670 AGLVSPTNALDPGLVFPTEYEDYINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNL 729

Query: 737 PSVTISSLVGHQVLHRRVKNVGTKVETYVWSVIPPNGTTININPPWFTIAPGGLQNLEIQ 796
           PS+TISSLVGHQV+ RRVKNVG KVETYV SVIPPNGTT+NI PPWFT+A   +QNLEIQ
Sbjct: 730 PSITISSLVGHQVVRRRVKNVGGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQ 789

Query: 797 IKVTHKMNHFTFGEILFTGSLNHIGRIPLSVLAVS 808
           I  THK +HFTFGEI  TGSLNHI RIPLS+L VS
Sbjct: 790 IIATHKTDHFTFGEIFLTGSLNHIARIPLSILVVS 794

BLAST of CmaCh18G005700 vs. NCBI nr
Match: gi|778665448|ref|XP_004145469.2| (PREDICTED: subtilisin-like protease SBT2.4 [Cucumis sativus])

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 580/784 (73.98%), Postives = 644/784 (82.14%), Query Frame = 1

Query: 29  LVLMEESSNEDHLLQNTLKSYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDRG 88
           ++L+E+S  +  +L+N++K+Y KL SFK I+NGFAVHTTPSEA+KLR+ANGVKLVE DRG
Sbjct: 15  MLLIEDSHKK--VLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRG 74

Query: 89  VRKMTTYTPKFLGVPSKSRLCSKKTKTSPDGEGIVIGFVDSGIDPTHPSFGFDDGYEDL- 148
           VRKMTTYTP+FLG+   +     K   S  G+GI+IGFVDSGI PTHPSF  + G ED  
Sbjct: 75  VRKMTTYTPEFLGLVKNNN--DYKYNYSGGGDGILIGFVDSGIYPTHPSFSNNFGKEDDD 134

Query: 149 RLFCEKGRFFPLSSCNGKIVGARFFSAGARAVAKLNSSVDFLSPFDVEGHGSHVASIAAG 208
            L CE+G  FP   CNGKIV ARFFSAGA+A A LNSS+DFLSPFD EGHGSHVASIAAG
Sbjct: 135 ELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGSHVASIAAG 194

Query: 209 NGGVPVYVNGFFYGFASGMAPRARIAVYKAVYPTMSTLTDVVSAIDQAVIDGVDILALSV 268
           N  VPV V+GFFYG A+G+AP ARIAVYKAVYPT++TLTDV+SAIDQAVIDGVDILALSV
Sbjct: 195 NAEVPVIVDGFFYGLATGIAPHARIAVYKAVYPTVATLTDVISAIDQAVIDGVDILALSV 254

Query: 269 GPNEPPEEGLTFLSIYDIAILSATRAGILVVQAVGNNGPASATVVSYSPWAVGVAASGTD 328
           GPNEP + G TFLSIYDIAILSATRAGILVVQA GNNGPA ATVVSYSPWA+GVAASGTD
Sbjct: 255 GPNEPSDVGFTFLSIYDIAILSATRAGILVVQAAGNNGPARATVVSYSPWAIGVAASGTD 314

Query: 329 RVYPASLLLGNGQKVGGVGLSGGSIHFCGYIFWDLYYSSQSNFLNKGFPLYSKHLSSSSG 388
           RVY  SLLLGNGQKVGGVG+SG S+                 FL+K              
Sbjct: 315 RVYSTSLLLGNGQKVGGVGMSGPSL-------------GSEFFLHK-------------- 374

Query: 389 PTFFLHKLVLAKDAMKQNG-TIPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAA 448
               L K    ++    N      EECQ+PEAFDPN+VQNSIVLCSFSQGFLNGTSSLAA
Sbjct: 375 --LVLAKDATKQNETNYNDIPSYIEECQHPEAFDPNIVQNSIVLCSFSQGFLNGTSSLAA 434

Query: 449 IIHTAMTLRFIGFALIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDAN 508
           IIHTA  L+F+GF LIANPNYGDFIAEPI FR+PGIL+P+ SD+Q IL YYE NTCKD  
Sbjct: 435 IIHTAKQLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVILKYYEENTCKDER 494

Query: 509 GMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIW 568
           GMV EFKGKA IGEGR+ASFG+QAPTVSRFSSRGPDY+N+NR++ADVLKPDILAPGHQIW
Sbjct: 495 GMVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIW 554

Query: 569 AAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATK 628
           AAWSPLS +EPLLKGY FALMSGTSMAAPHIVGIAALIKQK PSWTPSMIASAMSTTATK
Sbjct: 555 AAWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATK 614

Query: 629 YDMNGDLIQAEGFDIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAEYEDYINFLCSLPG- 688
           YDMNGDLIQAEGF++H LYPSTPFD GAGLV P+NALDPGLVFP EYED INFLCSLPG 
Sbjct: 615 YDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGV 674

Query: 689 DPAVIKNTTGGQCNASVPQPHPADLNLPSVTISSLVGHQVLHRRVKNVGTKVETYVWSVI 748
           DPAV+K+ TGGQCNAS+   HPADLNLPS+TISSLVGHQV+ RRVKNVG KVETYVWSVI
Sbjct: 675 DPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVI 734

Query: 749 PPNGTTININPPWFTIAPGGLQNLEIQIKVTHKMNHFTFGEILFTGSLNHIGRIPLSVLA 808
           PPNGTT+NINPP FT+A   +QNLEIQI  THK +HFTFGEI+ TGSLNHI RIPLS+L 
Sbjct: 735 PPNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILV 765

Query: 809 VSVS 810
           VS S
Sbjct: 795 VSAS 765

BLAST of CmaCh18G005700 vs. NCBI nr
Match: gi|1009108180|ref|XP_015883427.1| (PREDICTED: subtilisin-like protease SBT2.4 [Ziziphus jujuba])

HSP 1 Score: 951.8 bits (2459), Expect = 7.6e-274
Identity = 502/841 (59.69%), Postives = 607/841 (72.18%), Query Frame = 1

Query: 11  LIASLVLVYCFAEERQIYLVLMEESS--------------------------------NE 70
           LI  ++   CFAEER +YLV+ME  S                                + 
Sbjct: 8   LIIFVLFSACFAEERALYLVMMEGDSVAFPSNTSPSHEDGRTLDPHNVSKSHANRLVESH 67

Query: 71  DHLLQNTLK--SYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYT 130
           + LLQ+TL+  SY+KL SFKHI+NGFAVHTTPS+A KL+ A GV LVERDRG R MTTYT
Sbjct: 68  NQLLQSTLETGSYSKLYSFKHIVNGFAVHTTPSQAEKLKCAPGVNLVERDRGARLMTTYT 127

Query: 131 PKFLGVPSKSRLCSKKTKTSPDGEGIVIGFVDSGIDPTHPSFG-------FDDGYEDLRL 190
           PKFLG+  +  + +++      GEGIVIGFVD+GI+PTHPSF        F         
Sbjct: 128 PKFLGL--RKGVWTQEGGDRNAGEGIVIGFVDTGINPTHPSFAYHPHLNPFTSKLSRFSG 187

Query: 191 FCEKGRFFPLSSCNGKIVGARFFSAGARAVAKLNSSVDFLSPFDVEGHGSHVASIAAGNG 250
            CE G  FPLSSCNGKI+ ARFFSAGA+A+A LNSSVDFLSPFD  GHGSHVAS AAGN 
Sbjct: 188 ACETGPRFPLSSCNGKIISARFFSAGAQAIATLNSSVDFLSPFDAVGHGSHVASTAAGNA 247

Query: 251 GVPVYVNGFFYGFASGMAPRARIAVYKAVYPTMSTLTDVVSAIDQAVIDGVDILALSVGP 310
           GVPV VNGFFYG ASGMAPRA+IA+YKA+YPT+ TLTDVVSAIDQA+ DGVDIL LS+GP
Sbjct: 248 GVPVVVNGFFYGLASGMAPRAQIAIYKAIYPTVGTLTDVVSAIDQAIADGVDILTLSIGP 307

Query: 311 NEPPEEGLTFLSIYDIAILSATRAGILVVQAVGNNGPASATVVSYSPWAVGVAASGTDRV 370
           +EPPE+  TFL+I+DIA+L A RAG+ VVQA GN GP  ++VVSYSPWAVGVAAS TDR 
Sbjct: 308 DEPPEDSPTFLNIFDIAMLFARRAGVFVVQAAGNQGPGPSSVVSYSPWAVGVAASSTDRN 367

Query: 371 YPASLLLGNGQKVGGVGLSGGSIHFCGYIF-WDLYYSSQSNFLNKGFPLYSKHLSSSSGP 430
           +P SL+LGNGQK+GGVGLSG S  F G +F + L  +  +   N  FP    ++      
Sbjct: 368 FPGSLILGNGQKIGGVGLSGPS--FGGGLFLYKLVLARDAVKRNGTFPRTPPYI------ 427

Query: 431 TFFLHKLVLAKDAMKQNGTIPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAII 490
                                 EECQYPEA DPN+V++SIV+C FS GFLNG+S+L AII
Sbjct: 428 ----------------------EECQYPEALDPNVVKHSIVICIFSAGFLNGSSTLTAII 487

Query: 491 HTAMTLRFIGFALIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDANGM 550
            TA TL F+GF L+ANPN+GDFIAEP+ F + GILIP  + +Q I NYYE  T +D  G+
Sbjct: 488 DTAETLGFMGFVLVANPNFGDFIAEPLPFDISGILIPTVAGAQVISNYYEQKTYRDGKGL 547

Query: 551 VSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAA 610
           V+ F  +AAIGEGR+A F  Q PTVSRFSSRGPD++++ R  ADVLKPDILAPGHQ+WAA
Sbjct: 548 VTRFTARAAIGEGRIACFKDQVPTVSRFSSRGPDFIDVKRNPADVLKPDILAPGHQVWAA 607

Query: 611 WSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYD 670
           WSP+S  EP+L GY F L+SGTSMA PH+ GIAAL+KQ NPSWTPSMIASAM+TTA+KYD
Sbjct: 608 WSPISALEPILMGYNFGLLSGTSMATPHVAGIAALLKQYNPSWTPSMIASAMATTASKYD 667

Query: 671 MNGDLIQAEGFDIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAEYEDYINFLCSLPG-DP 730
            +GDLI+AEG  I  LYPSTPFD G+GL+ P +AL PGLVF +EYEDY+ FLCSLP  +P
Sbjct: 668 NDGDLIKAEGSSIGSLYPSTPFDFGSGLLSPKSALHPGLVFSSEYEDYVGFLCSLPDIEP 727

Query: 731 AVIKNTTGGQCNASVPQPHPADLNLPSVTISSLVGHQVLHRRVKNVGTKVETYVWSVIPP 790
           A+I+N TG  CN S+   HPA+LN+PS+TISSL+G Q++ R VKNV +K ETY+ SV+PP
Sbjct: 728 AMIRNATGEWCNYSL--GHPANLNVPSITISSLIGSQLVQRTVKNVESKAETYLCSVLPP 787

Query: 791 NGTTININPPWFTIAPGGLQNLEIQIKVTHKMNHFTFGEILFTGSLNHIGRIPLSVLAVS 809
           NGT +N+ PPWFTIAP   Q+L +Q  VT  +  F+FGEI+ TGSLNH+ RIPLS+L VS
Sbjct: 788 NGTVVNLYPPWFTIAPQETQDLYMQFNVTEAIGEFSFGEIILTGSLNHVVRIPLSILPVS 814

BLAST of CmaCh18G005700 vs. NCBI nr
Match: gi|255554773|ref|XP_002518424.1| (PREDICTED: subtilisin-like protease SBT2.4 isoform X2 [Ricinus communis])

HSP 1 Score: 941.4 bits (2432), Expect = 1.0e-270
Identity = 501/844 (59.36%), Postives = 609/844 (72.16%), Query Frame = 1

Query: 6   STLSGLIASLVLVYCFAEERQIYLVLME----------------------ESS------- 65
           ST S L+  ++ V C AEER IYLVLME                      E+S       
Sbjct: 6   STYSLLLVFVISVACSAEERSIYLVLMEGQPVAFLGGHEPYTTRKLELNSEASQAHARRL 65

Query: 66  --NEDHLLQNTLK--SYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKM 125
             + D LLQ+TL+  SYTKL SFKHI+NGFAVH T S+A KL+ A GVK+VERDRG + M
Sbjct: 66  VDSHDQLLQSTLEIGSYTKLYSFKHIVNGFAVHATHSQAKKLKDAPGVKVVERDRGAKLM 125

Query: 126 TTYTPKFLGVPSKSRLCSKKTKTSPDGEGIVIGFVDSGIDPTHPSFG------FDDGYED 185
           TTYTP+FL +     + +++      GEGIVIGF+D+GI+P HPSF       F      
Sbjct: 126 TTYTPQFLEL--SQGVWTQEGGDRNAGEGIVIGFIDTGINPLHPSFAYNPLNPFTSNISH 185

Query: 186 LRLFCEKGRFFPLSSCNGKIVGARFFSAGARAVAKLNSSVDFLSPFDVEGHGSHVASIAA 245
               CE G  FP  SCNGKIV ARFFSAGA+AV+ LN+S+DFLSP+D  GHGSHVAS AA
Sbjct: 186 FSGACETGPRFPAGSCNGKIVSARFFSAGAQAVSPLNTSLDFLSPYDAVGHGSHVASTAA 245

Query: 246 GNGGVPVYVNGFFYGFASGMAPRARIAVYKAVYPTMSTLTDVVSAIDQAVIDGVDILALS 305
           GN  VPV  NGF+YG ASGMAPRARIAVYKA+YPT+ TLTDV++AIDQA  DGVDI+ LS
Sbjct: 246 GNARVPVVANGFYYGRASGMAPRARIAVYKAIYPTVGTLTDVIAAIDQATKDGVDIITLS 305

Query: 306 VGPNEPPEEGLTFLSIYDIAILSATRAGILVVQAVGNNGPASATVVSYSPWAVGVAASGT 365
           VGP+EPPE+ +TFLS++D+ +L A RAG+ VVQA GN+GP+ +TVVSYSPWAVGVAAS T
Sbjct: 306 VGPDEPPEDTITFLSVFDVFMLFAQRAGVFVVQAAGNHGPSLSTVVSYSPWAVGVAASTT 365

Query: 366 DRVYPASLLLGNGQKVGGVGLSGGSIHFCGYIFWDLYYSSQSNFLNKGFPLYSKHLSSSS 425
           DR+YPASLLLGNGQKVGGVGLSG +  + G   + L ++  +   N  FP   +++    
Sbjct: 366 DRIYPASLLLGNGQKVGGVGLSGPTFGY-GLFKYKLVFAQDAVKANGTFPRTPQYI---- 425

Query: 426 GPTFFLHKLVLAKDAMKQNGTIPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAA 485
                                   EECQ+PE+ DP LV+  IV+C+FS GF NGTSS+ A
Sbjct: 426 ------------------------EECQHPESLDPKLVRRRIVICTFSAGFYNGTSSITA 485

Query: 486 IIHTAMTLRFIGFALIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDAN 545
           II T+ TLRF GFAL+ANP+YGDFIAEPI F +PGI+IP  +D++ I  YYE    +D  
Sbjct: 486 IIDTSRTLRFTGFALVANPSYGDFIAEPIPFAVPGIMIPKVADAEIISKYYEQEILRDER 545

Query: 546 GMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIW 605
           G VS+F  + AIGEGRVA+F  +AP VSRFSSRGPD++++NR  ADVLKPDILAPGHQIW
Sbjct: 546 GFVSKFCARGAIGEGRVAAFEGRAPIVSRFSSRGPDFLDINRIPADVLKPDILAPGHQIW 605

Query: 606 AAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATK 665
           AAWSPLS  +P+L G  FAL+SGTSMA PHIVGIAALIKQ +PSWTPSMIASA+STTA  
Sbjct: 606 AAWSPLSALDPILTGDNFALLSGTSMATPHIVGIAALIKQFHPSWTPSMIASALSTTAGN 665

Query: 666 YDMNGDLIQAEGFDIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAEYEDYINFLCSLPG- 725
           YD  G+LI AEGFDI+ LYPST FDLGAG V PT A+DPGLVFP+E+++YI+FLCSLPG 
Sbjct: 666 YDNYGELILAEGFDINSLYPSTHFDLGAGFVNPTRAMDPGLVFPSEFQNYISFLCSLPGI 725

Query: 726 DPAVIKNTTGGQCNASVPQPHPADLNLPSVTISSLVGHQVLHRRVKNVGTKVETYVWSVI 785
           DPA++K TTG  CN S+    PA+LNLPSVTIS+L G Q + R VKNVG K ETY+ SVI
Sbjct: 726 DPAIVKATTGEPCNQSLSS--PANLNLPSVTISALRGSQTVERNVKNVGIKPETYLSSVI 785

Query: 786 PPNGTTININPPWFTIAPGGLQNLEIQIKVTHKMNHFTFGEILFTGSLNHIGRIPLSVLA 810
            PNGTT+N++P WF IAP G QN++I+  VTH  N F+FG+I+ TGSL+HI RIPLS+L 
Sbjct: 786 APNGTTVNLSPTWFIIAPQGTQNIDIEFHVTHARNEFSFGQIVLTGSLDHIVRIPLSILP 816

BLAST of CmaCh18G005700 vs. NCBI nr
Match: gi|1000967753|ref|XP_015574292.1| (PREDICTED: subtilisin-like protease SBT2.4 isoform X1 [Ricinus communis])

HSP 1 Score: 937.9 bits (2423), Expect = 1.1e-269
Identity = 502/845 (59.41%), Postives = 609/845 (72.07%), Query Frame = 1

Query: 6   STLSGLIASLVLVYCFAEERQIYLVLME----------------------ESS------- 65
           ST S L+  ++ V C AEER IYLVLME                      E+S       
Sbjct: 6   STYSLLLVFVISVACSAEERSIYLVLMEGQPVAFLGGHEPYTTRKLELNSEASQAHARRL 65

Query: 66  --NEDHLLQNTLK--SYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKM 125
             + D LLQ+TL+  SYTKL SFKHI+NGFAVH T S+A KL+ A GVK+VERDRG + M
Sbjct: 66  VDSHDQLLQSTLEIGSYTKLYSFKHIVNGFAVHATHSQAKKLKDAPGVKVVERDRGAKLM 125

Query: 126 TTYTPKFLGVPSKSRLCSKKTKTSPDGEGIVIGFVDSGIDPTHPSFG------FDDGYED 185
           TTYTP+FL +     + +++      GEGIVIGF+D+GI+P HPSF       F      
Sbjct: 126 TTYTPQFLEL--SQGVWTQEGGDRNAGEGIVIGFIDTGINPLHPSFAYNPLNPFTSNISH 185

Query: 186 LRLFCEKGRFFPLSSCNGKIVGARFFSAGARAVAKLNSSVDFLSPFDVEGHGSHVASIAA 245
               CE G  FP  SCNGKIV ARFFSAGA+AV+ LN+S+DFLSP+D  GHGSHVAS AA
Sbjct: 186 FSGACETGPRFPAGSCNGKIVSARFFSAGAQAVSPLNTSLDFLSPYDAVGHGSHVASTAA 245

Query: 246 GNGGVPVYVNGFFYGFASGMAPRARIAVYKAVYPTMSTLTDVVSAIDQAVIDGVDILALS 305
           GN  VPV  NGF+YG ASGMAPRARIAVYKA+YPT+ TLTDV++AIDQA  DGVDI+ LS
Sbjct: 246 GNARVPVVANGFYYGRASGMAPRARIAVYKAIYPTVGTLTDVIAAIDQATKDGVDIITLS 305

Query: 306 VGPNEPPEEGLTFLSIYDIAILSATRAGILVVQAVGNNGPASATVVSYSPWAVGVAASGT 365
           VGP+EPPE+ +TFLS++D+ +L A RAG+ VVQA GN+GP+ +TVVSYSPWAVGVAAS T
Sbjct: 306 VGPDEPPEDTITFLSVFDVFMLFAQRAGVFVVQAAGNHGPSLSTVVSYSPWAVGVAASTT 365

Query: 366 DRVYPASLLLGNGQKVGGVGLSGGSIHFCGYIFWDLYYSSQSNFLNKGFPLYSKHLSSSS 425
           DR+YPASLLLGNGQKVGGVGLSG +  + G   + L ++  +   N  FP   +++    
Sbjct: 366 DRIYPASLLLGNGQKVGGVGLSGPTFGY-GLFKYKLVFAQDAVKANGTFPRTPQYI---- 425

Query: 426 GPTFFLHKLVLAKDAMKQNGTIPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAA 485
                                   EECQ+PE+ DP LV+  IV+C+FS GF NGTSS+ A
Sbjct: 426 ------------------------EECQHPESLDPKLVRRRIVICTFSAGFYNGTSSITA 485

Query: 486 IIHTAMTLRFIGFALIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDAN 545
           II T+ TLRF GFAL+ANP+YGDFIAEPI F +PGI+IP  +D++ I  YYE    +D  
Sbjct: 486 IIDTSRTLRFTGFALVANPSYGDFIAEPIPFAVPGIMIPKVADAEIISKYYEQEILRDER 545

Query: 546 GMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIW 605
           G VS+F  + AIGEGRVA+F  +AP VSRFSSRGPD++++NR  ADVLKPDILAPGHQIW
Sbjct: 546 GFVSKFCARGAIGEGRVAAFEGRAPIVSRFSSRGPDFLDINRIPADVLKPDILAPGHQIW 605

Query: 606 AAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATK 665
           AAWSPLS  +P+L G  FAL+SGTSMA PHIVGIAALIKQ +PSWTPSMIASA+STTA  
Sbjct: 606 AAWSPLSALDPILTGDNFALLSGTSMATPHIVGIAALIKQFHPSWTPSMIASALSTTAGN 665

Query: 666 YDMNGDLIQAEGFDIHRLYPSTPFDLGAGLVKPTNALDPGLVFP-AEYEDYINFLCSLPG 725
           YD  G+LI AEGFDI+ LYPST FDLGAG V PT A+DPGLVFP AE+++YI+FLCSLPG
Sbjct: 666 YDNYGELILAEGFDINSLYPSTHFDLGAGFVNPTRAMDPGLVFPSAEFQNYISFLCSLPG 725

Query: 726 -DPAVIKNTTGGQCNASVPQPHPADLNLPSVTISSLVGHQVLHRRVKNVGTKVETYVWSV 785
            DPA++K TTG  CN S+    PA+LNLPSVTIS+L G Q + R VKNVG K ETY+ SV
Sbjct: 726 IDPAIVKATTGEPCNQSLSS--PANLNLPSVTISALRGSQTVERNVKNVGIKPETYLSSV 785

Query: 786 IPPNGTTININPPWFTIAPGGLQNLEIQIKVTHKMNHFTFGEILFTGSLNHIGRIPLSVL 810
           I PNGTT+N++P WF IAP G QN++I+  VTH  N F+FG+I+ TGSL+HI RIPLS+L
Sbjct: 786 IAPNGTTVNLSPTWFIIAPQGTQNIDIEFHVTHARNEFSFGQIVLTGSLDHIVRIPLSIL 817