CSPI01G34220 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI01G34220
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionSubtilisin-like protease
LocationChr1 : 29112581 .. 29113144 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGATGATGGTGGTGATGAACTAAGAAGCAACAAAGAAATGTTGTTAATAGAAGATTCACACAAGAAGGTTTTAGAAAACAGTATAAAAAACTACAGAAAGCTGCATAGCTTCAAGAAAATTTTGAATGGTTTTGCAGTCCACACAACACCATCAGAAGCTTCAAAGCTCAGAGAAGCTAATGGAGTGAAATTGGTTGAGTTAGATAGAGGAGTGAGGAAAATGACGACTTATACCCCTGAGTTTTTAGGGTTAGTGAAGAATAATAATGATTATAAATATAATTATAGTGGTGGTGGGGATGGGATTTTGATTGGGTTTGTGGATTCTGGGATTTATCCAACACATCCAAGTTTTAGCAATAACTTTGGTAAAGAAGATGATGATGAGTTGGTTTGTGAAGAAGGTCCTTTATTTCCAAAAGGTTGTTGCAATGGGAAGATAGTGAGTGCTAGGTTTTTCTCTGCTGGGGCTCAGGCTGCTGCAGTGCTTAATTCTTCTATTGATTTCCTATCTCCATTTGATGCTGAAGGCCATGGAAGGTATCCGATCCTAGCTTAG

mRNA sequence

ATGGAAGATGATGGTGGTGATGAACTAAGAAGCAACAAAGAAATGTTGTTAATAGAAGATTCACACAAGAAGGTTTTAGAAAACAGTATAAAAAACTACAGAAAGCTGCATAGCTTCAAGAAAATTTTGAATGGTTTTGCAGTCCACACAACACCATCAGAAGCTTCAAAGCTCAGAGAAGCTAATGGAGTGAAATTGGTTGAGTTAGATAGAGGAGTGAGGAAAATGACGACTTATACCCCTGAGTTTTTAGGGTTAGTGAAGAATAATAATGATTATAAATATAATTATAGTGGTGGTGGGGATGGGATTTTGATTGGGTTTGTGGATTCTGGGATTTATCCAACACATCCAAGTTTTAGCAATAACTTTGGTAAAGAAGATGATGATGAGTTGGTTTGTGAAGAAGGTCCTTTATTTCCAAAAGGTTGTTGCAATGGGAAGATAGTGAGTGCTAGGTTTTTCTCTGCTGGGGCTCAGGCTGCTGCAGTGCTTAATTCTTCTATTGATTTCCTATCTCCATTTGATGCTGAAGGCCATGGAAGGTATCCGATCCTAGCTTAG

Coding sequence (CDS)

ATGGAAGATGATGGTGGTGATGAACTAAGAAGCAACAAAGAAATGTTGTTAATAGAAGATTCACACAAGAAGGTTTTAGAAAACAGTATAAAAAACTACAGAAAGCTGCATAGCTTCAAGAAAATTTTGAATGGTTTTGCAGTCCACACAACACCATCAGAAGCTTCAAAGCTCAGAGAAGCTAATGGAGTGAAATTGGTTGAGTTAGATAGAGGAGTGAGGAAAATGACGACTTATACCCCTGAGTTTTTAGGGTTAGTGAAGAATAATAATGATTATAAATATAATTATAGTGGTGGTGGGGATGGGATTTTGATTGGGTTTGTGGATTCTGGGATTTATCCAACACATCCAAGTTTTAGCAATAACTTTGGTAAAGAAGATGATGATGAGTTGGTTTGTGAAGAAGGTCCTTTATTTCCAAAAGGTTGTTGCAATGGGAAGATAGTGAGTGCTAGGTTTTTCTCTGCTGGGGCTCAGGCTGCTGCAGTGCTTAATTCTTCTATTGATTTCCTATCTCCATTTGATGCTGAAGGCCATGGAAGGTATCCGATCCTAGCTTAG
Homology
[Close]
BLAST of CSPI01G34220 vs. Swiss-Prot
Match: SBT24_ARATH (Subtilisin-like protease SBT2.4 OS=Arabidopsis thaliana GN=SBT2.4 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 2.0e-39
Identity = 91/184 (49.46%), Postives = 119/184 (64.67%), Query Frame = 1 

Query: 18  IEDSHKKVLENSIK--NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRK 77
           IE+ H ++L ++++  +Y KL+SFK ++N  AV TT S+A KL +  GVK VE D+GV+ 
Sbjct: 78  IEEIHDEILGSTLEKGSYTKLYSFKHVINAIAVRTTASQAKKLGKTKGVKAVEEDKGVKL 137

Query: 78  MTTYTPEFLGL-------VKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF-------- 137
           MTTYTP+FL L       + N  D +      G+ I+IGFVD+GI PTHPSF        
Sbjct: 138 MTTYTPDFLELPQQVWQKISNEGDRR-----AGEDIVIGFVDTGINPTHPSFAALDLTNP 197

Query: 138 -SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEG 184
            S+N  +       CE GP FP G CNGKI+SARFFSAGA+A+  LNSS+D LSPFDA G
Sbjct: 198 YSSNLSRLHFSG-DCEIGPFFPPGSCNGKIISARFFSAGARASGALNSSLDILSPFDASG 255

[Close]
BLAST of CSPI01G34220 vs. Swiss-Prot
Match: SBT26_ARATH (Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana GN=SBT2.6 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.7e-33
Identity = 71/157 (45.22%), Postives = 99/157 (63.06%), Query Frame = 1 

Query: 32  NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRKMTTYTPEFLGLVKNNN 91
           +Y+KL+S+K ++NGFA H +P +A  LR A GVK V+ D  VRK+TT+TP+FLGL  +  
Sbjct: 82  SYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRDWKVRKLTTHTPQFLGLPTDVW 141

Query: 92  DYKYNYSGGGDGILIGFVDSGIYPTHPSFSNN-----FGKEDDDELVCEEGPLFPKGCCN 151
                Y   G+ I+IGF+DSGI+P HPSF+++     +G     +  CEE P      CN
Sbjct: 142 PTGGGYDRAGEDIVIGFIDSGIFPHHPSFASHHTTVPYGPHPSYKGKCEEDPHTKISFCN 201

Query: 152 GKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
           GKI+ A+ F+  A+AA   N  IDF SP D +GHG +
Sbjct: 202 GKIIGAQHFAEAAKAAGAFNPDIDFASPMDGDGHGSH 238

[Close]
BLAST of CSPI01G34220 vs. Swiss-Prot
Match: SBT25_ARATH (Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana GN=SBT2.5 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 1.8e-32
Identity = 71/156 (45.51%), Postives = 99/156 (63.46%), Query Frame = 1 

Query: 32  NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRKMTTYTPEFLGLVKNNN 91
           +Y+KL+S+K ++NGFA H +P +A  LR A GV+ V+ D  VR++TT+TPEFLGL  +  
Sbjct: 82  SYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRLTTHTPEFLGLPTDVW 141

Query: 92  DYKYNYSGGGDGILIGFVDSGIYPTHPSFSNN----FGKEDDDELVCEEGPLFPKGCCNG 151
                +   G+ I+IGFVDSGIYP HPSF+++    +G     +  CEE P   K  CN 
Sbjct: 142 PTGGGFDRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLPHYKGKCEEDPHTKKSFCNR 201

Query: 152 KIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
           KIV A+ F+  A+AA   N  ID+ SP D +GHG +
Sbjct: 202 KIVGAQHFAEAAKAAGAFNPDIDYASPMDGDGHGSH 237

[Close]
BLAST of CSPI01G34220 vs. Swiss-Prot
Match: SBT22_ARATH (Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana GN=SBT2.2 PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 7.5e-31
Identity = 73/174 (41.95%), Postives = 99/174 (56.90%), Query Frame = 1 

Query: 18  IEDSHKKVLENSIK--NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRK 77
           I  +H  +L N++K   Y KL+SF  ++NGFAV  +  +A  L     V  + LD  VR 
Sbjct: 99  IAQAHDSLLRNALKGEKYIKLYSFHYLINGFAVFVSSQQAETLSRRREVANIVLDFSVRT 158

Query: 78  MTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSFSNNFGKEDDDEL--- 137
            TTYTP+F+GL K     +  Y   G+GI+IGF+D+GI PTHPSF+     +    +   
Sbjct: 159 ATTYTPQFMGLPKGAWVKEGGYETAGEGIVIGFIDTGIDPTHPSFNGTDTSQRQYPIPNH 218

Query: 138 ---VCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
              VCE  P FP G CN K+V AR F+  A    + NSS D+ SPFD +GHG +
Sbjct: 219 FSGVCEVTPDFPSGSCNRKLVGARHFAQSAITRGIFNSSEDYASPFDGDGHGTH 272

[Close]
BLAST of CSPI01G34220 vs. Swiss-Prot
Match: SBT23_ARATH (Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana GN=SBT2.3 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 5.3e-29
Identity = 68/173 (39.31%), Postives = 97/173 (56.07%), Query Frame = 1 

Query: 18  IEDSHKKVLENSIK--NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRK 77
           +  SH   L  ++K   Y KL+S+  ++NGFA+     +A KL     V  + LD  VR 
Sbjct: 86  VVQSHDSFLRKTLKGEKYIKLYSYHYLINGFALFINSQQAEKLSMRKEVANIVLDYSVRT 145

Query: 78  MTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSFSNNFGKED-----DD 137
            TTYTP+F+GL +     +  +   G+G++IGF+D+GI P HPSF++N  K         
Sbjct: 146 ATTYTPQFMGLPQGAWVKEGGFEIAGEGVIIGFIDTGIDPNHPSFNDNDSKRSYPIPKHF 205

Query: 138 ELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
             VCE  P FP G CN K++ AR F+  A    + NSS D+ SPFD +GHG +
Sbjct: 206 SGVCEVTPDFPSGSCNKKLIGARHFAQSAVTRGIFNSSEDYASPFDGDGHGTH 258

[Close]
BLAST of CSPI01G34220 vs. TrEMBL
Match: A0A0A0LYI5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G701240 PE=4 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 6.9e-100
Identity = 181/183 (98.91%), Postives = 182/183 (99.45%), Query Frame = 1 

Query: 1   MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60
           MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE
Sbjct: 1   MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60

Query: 61  ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF 120
           ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF
Sbjct: 61  ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF 120

Query: 121 SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGH 180
           SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGH
Sbjct: 121 SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGH 180

Query: 181 GRY 184
           G +
Sbjct: 181 GSH 183

[Close]
BLAST of CSPI01G34220 vs. TrEMBL
Match: W9RY25_9ROSA (Subtilisin-like protease OS=Morus notabilis GN=L484_014157 PE=3 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 2.6e-46
Identity = 100/171 (58.48%), Postives = 124/171 (72.51%), Query Frame = 1 

Query: 20  DSHKKVLENSIK--NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRKMT 79
           +SH K+L+++++  +Y KLHSFK I+NGFAVHTTPS+ASKLR+  GV+LVE DRG + MT
Sbjct: 71  NSHDKLLQSTLETGSYTKLHSFKHIVNGFAVHTTPSQASKLRDVEGVRLVEKDRGAKLMT 130

Query: 80  TYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSFSNNFGKEDDDEL----- 139
           TYTP+FLGL +     +      G+GI+IGFVD+GI PTHPSF+ N        L     
Sbjct: 131 TYTPKFLGLREGVWAQEGGERNAGEGIVIGFVDTGINPTHPSFAFNPFNPFTSNLSRFSG 190

Query: 140 VCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
            CE GP FP   CNGKIVSARFFSAGAQAA  LN+S+DFLSP+DA GHG +
Sbjct: 191 ACEAGPHFPASSCNGKIVSARFFSAGAQAATTLNTSVDFLSPYDAVGHGSH 241

[Close]
BLAST of CSPI01G34220 vs. TrEMBL
Match: B9HZB1_POPTR (Abnormal leaf shape family protein (Fragment) OS=Populus trichocarpa GN=POPTR_0011s01560g PE=3 SV=2)

HSP 1 Score: 189.1 bits (479), Expect = 4.8e-45
Identity = 99/171 (57.89%), Postives = 124/171 (72.51%), Query Frame = 1 

Query: 20  DSHKKVLENSIK--NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRKMT 79
           DSH ++L++++K  +Y KL+SFK I+NGF+VHTTPS+A+KL+ A GVKLVE DRG + MT
Sbjct: 45  DSHDQLLQSNLKTGSYNKLYSFKHIVNGFSVHTTPSQANKLKVAPGVKLVEKDRGAKLMT 104

Query: 80  TYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSFS----NNFGKE-DDDEL 139
           TYTP+FLGL +     +     GG+GI+IGFVD+GI P HPSF+    N F         
Sbjct: 105 TYTPQFLGLPQEVWAKEGGDKNGGEGIVIGFVDTGITPEHPSFTYDPLNPFTSNISHFSG 164

Query: 140 VCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
            CE GP FP   CNGKIVSAR+FSAGAQA A LN+S+DFLSPFDA GHG +
Sbjct: 165 ACETGPRFPSSSCNGKIVSARYFSAGAQAIATLNTSVDFLSPFDAAGHGSH 215

[Close]
BLAST of CSPI01G34220 vs. TrEMBL
Match: I1KNH1_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_07G263500 PE=3 SV=2)

HSP 1 Score: 184.1 bits (466), Expect = 1.6e-43
Identity = 96/173 (55.49%), Postives = 121/173 (69.94%), Query Frame = 1 

Query: 21  SHKKVLENSIKN--YRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRKMTT 80
           SH  +L++S++N  Y KLHS+K I+NGF+VHTTPS+A++LR + GVKLVE DRG +  TT
Sbjct: 75  SHDLLLQSSLENGSYNKLHSYKHIINGFSVHTTPSQAARLRRSPGVKLVEKDRGAKMRTT 134

Query: 81  YTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPS--------FSNNFGKEDDD 140
           YTPEFL L K     +      G+G++IGFVDSGI   HPS        FS+N  +    
Sbjct: 135 YTPEFLSLRKGIWAQEGGERNAGEGVVIGFVDSGINALHPSFAYDPMHPFSSNLSR---F 194

Query: 141 ELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
           E  CE GPLFP   CNGKIV+ARFFSAGA+A   LN+S+DFLSPFDA+GHG +
Sbjct: 195 EGACETGPLFPPSSCNGKIVAARFFSAGAEATVTLNASMDFLSPFDADGHGSH 244

[Close]
BLAST of CSPI01G34220 vs. TrEMBL
Match: A0A0B2RV96_GLYSO (Subtilisin-like protease (Fragment) OS=Glycine soja GN=glysoja_039498 PE=3 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.6e-43
Identity = 96/173 (55.49%), Postives = 121/173 (69.94%), Query Frame = 1 

Query: 21  SHKKVLENSIKN--YRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRKMTT 80
           SH  +L++S++N  Y KLHS+K I+NGF+VHTTPS+A++LR + GVKLVE DRG +  TT
Sbjct: 13  SHDLLLQSSLENGSYNKLHSYKHIINGFSVHTTPSQAARLRRSPGVKLVEKDRGAKMRTT 72

Query: 81  YTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPS--------FSNNFGKEDDD 140
           YTPEFL L K     +      G+G++IGFVDSGI   HPS        FS+N  +    
Sbjct: 73  YTPEFLSLRKGIWAQEGGERNAGEGVVIGFVDSGINALHPSFAYDPMHPFSSNLSR---F 132

Query: 141 ELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
           E  CE GPLFP   CNGKIV+ARFFSAGA+A   LN+S+DFLSPFDA+GHG +
Sbjct: 133 EGACETGPLFPPSSCNGKIVAARFFSAGAEATVTLNASMDFLSPFDADGHGSH 182

[Close]
BLAST of CSPI01G34220 vs. TAIR10
Match: AT1G62340.1 (AT1G62340.1 PA-domain containing subtilase family protein)

HSP 1 Score: 163.7 bits (413), Expect = 1.1e-40
Identity = 91/184 (49.46%), Postives = 119/184 (64.67%), Query Frame = 1 

Query: 18  IEDSHKKVLENSIK--NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRK 77
           IE+ H ++L ++++  +Y KL+SFK ++N  AV TT S+A KL +  GVK VE D+GV+ 
Sbjct: 78  IEEIHDEILGSTLEKGSYTKLYSFKHVINAIAVRTTASQAKKLGKTKGVKAVEEDKGVKL 137

Query: 78  MTTYTPEFLGL-------VKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF-------- 137
           MTTYTP+FL L       + N  D +      G+ I+IGFVD+GI PTHPSF        
Sbjct: 138 MTTYTPDFLELPQQVWQKISNEGDRR-----AGEDIVIGFVDTGINPTHPSFAALDLTNP 197

Query: 138 -SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEG 184
            S+N  +       CE GP FP G CNGKI+SARFFSAGA+A+  LNSS+D LSPFDA G
Sbjct: 198 YSSNLSRLHFSG-DCEIGPFFPPGSCNGKIISARFFSAGARASGALNSSLDILSPFDASG 255

[Close]
BLAST of CSPI01G34220 vs. TAIR10
Match: AT4G30020.1 (AT4G30020.1 PA-domain containing subtilase family protein)

HSP 1 Score: 143.3 bits (360), Expect = 1.5e-34
Identity = 71/157 (45.22%), Postives = 99/157 (63.06%), Query Frame = 1 

Query: 32  NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRKMTTYTPEFLGLVKNNN 91
           +Y+KL+S+K ++NGFA H +P +A  LR A GVK V+ D  VRK+TT+TP+FLGL  +  
Sbjct: 82  SYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRDWKVRKLTTHTPQFLGLPTDVW 141

Query: 92  DYKYNYSGGGDGILIGFVDSGIYPTHPSFSNN-----FGKEDDDELVCEEGPLFPKGCCN 151
                Y   G+ I+IGF+DSGI+P HPSF+++     +G     +  CEE P      CN
Sbjct: 142 PTGGGYDRAGEDIVIGFIDSGIFPHHPSFASHHTTVPYGPHPSYKGKCEEDPHTKISFCN 201

Query: 152 GKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
           GKI+ A+ F+  A+AA   N  IDF SP D +GHG +
Sbjct: 202 GKIIGAQHFAEAAKAAGAFNPDIDFASPMDGDGHGSH 238

[Close]
BLAST of CSPI01G34220 vs. TAIR10
Match: AT2G19170.1 (AT2G19170.1 subtilisin-like serine protease 3)

HSP 1 Score: 140.6 bits (353), Expect = 1.0e-33
Identity = 71/156 (45.51%), Postives = 99/156 (63.46%), Query Frame = 1 

Query: 32  NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRKMTTYTPEFLGLVKNNN 91
           +Y+KL+S+K ++NGFA H +P +A  LR A GV+ V+ D  VR++TT+TPEFLGL  +  
Sbjct: 82  SYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRLTTHTPEFLGLPTDVW 141

Query: 92  DYKYNYSGGGDGILIGFVDSGIYPTHPSFSNN----FGKEDDDELVCEEGPLFPKGCCNG 151
                +   G+ I+IGFVDSGIYP HPSF+++    +G     +  CEE P   K  CN 
Sbjct: 142 PTGGGFDRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLPHYKGKCEEDPHTKKSFCNR 201

Query: 152 KIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
           KIV A+ F+  A+AA   N  ID+ SP D +GHG +
Sbjct: 202 KIVGAQHFAEAAKAAGAFNPDIDYASPMDGDGHGSH 237

[Close]
BLAST of CSPI01G34220 vs. TAIR10
Match: AT4G20430.1 (AT4G20430.1 Subtilase family protein)

HSP 1 Score: 135.2 bits (339), Expect = 4.2e-32
Identity = 73/174 (41.95%), Postives = 99/174 (56.90%), Query Frame = 1 

Query: 18  IEDSHKKVLENSIK--NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRK 77
           I  +H  +L N++K   Y KL+SF  ++NGFAV  +  +A  L     V  + LD  VR 
Sbjct: 99  IAQAHDSLLRNALKGEKYIKLYSFHYLINGFAVFVSSQQAETLSRRREVANIVLDFSVRT 158

Query: 78  MTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSFSNNFGKEDDDEL--- 137
            TTYTP+F+GL K     +  Y   G+GI+IGF+D+GI PTHPSF+     +    +   
Sbjct: 159 ATTYTPQFMGLPKGAWVKEGGYETAGEGIVIGFIDTGIDPTHPSFNGTDTSQRQYPIPNH 218

Query: 138 ---VCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
              VCE  P FP G CN K+V AR F+  A    + NSS D+ SPFD +GHG +
Sbjct: 219 FSGVCEVTPDFPSGSCNRKLVGARHFAQSAITRGIFNSSEDYASPFDGDGHGTH 272

[Close]
BLAST of CSPI01G34220 vs. TAIR10
Match: AT5G44530.1 (AT5G44530.1 Subtilase family protein)

HSP 1 Score: 129.0 bits (323), Expect = 3.0e-30
Identity = 68/173 (39.31%), Postives = 97/173 (56.07%), Query Frame = 1 

Query: 18  IEDSHKKVLENSIK--NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRK 77
           +  SH   L  ++K   Y KL+S+  ++NGFA+     +A KL     V  + LD  VR 
Sbjct: 86  VVQSHDSFLRKTLKGEKYIKLYSYHYLINGFALFINSQQAEKLSMRKEVANIVLDYSVRT 145

Query: 78  MTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSFSNNFGKED-----DD 137
            TTYTP+F+GL +     +  +   G+G++IGF+D+GI P HPSF++N  K         
Sbjct: 146 ATTYTPQFMGLPQGAWVKEGGFEIAGEGVIIGFIDTGIDPNHPSFNDNDSKRSYPIPKHF 205

Query: 138 ELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
             VCE  P FP G CN K++ AR F+  A    + NSS D+ SPFD +GHG +
Sbjct: 206 SGVCEVTPDFPSGSCNKKLIGARHFAQSAVTRGIFNSSEDYASPFDGDGHGTH 258

[Close]
BLAST of CSPI01G34220 vs. NCBI nr
Match: gi|778665448|ref|XP_004145469.2| (PREDICTED: subtilisin-like protease SBT2.4 [Cucumis sativus])

HSP 1 Score: 371.3 bits (952), Expect = 9.9e-100
Identity = 181/183 (98.91%), Postives = 182/183 (99.45%), Query Frame = 1 

Query: 1   MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60
           MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE
Sbjct: 1   MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60

Query: 61  ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF 120
           ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF
Sbjct: 61  ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF 120

Query: 121 SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGH 180
           SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGH
Sbjct: 121 SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGH 180

Query: 181 GRY 184
           G +
Sbjct: 181 GSH 183

[Close]
BLAST of CSPI01G34220 vs. NCBI nr
Match: gi|700211752|gb|KGN66848.1| (hypothetical protein Csa_1G701240 [Cucumis sativus])

HSP 1 Score: 371.3 bits (952), Expect = 9.9e-100
Identity = 181/183 (98.91%), Postives = 182/183 (99.45%), Query Frame = 1 

Query: 1   MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60
           MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE
Sbjct: 1   MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60

Query: 61  ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF 120
           ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF
Sbjct: 61  ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF 120

Query: 121 SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGH 180
           SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGH
Sbjct: 121 SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGH 180

Query: 181 GRY 184
           G +
Sbjct: 181 GSH 183

[Close]
BLAST of CSPI01G34220 vs. NCBI nr
Match: gi|659118464|ref|XP_008459133.1| (PREDICTED: LOW QUALITY PROTEIN: cucumisin, partial [Cucumis melo])

HSP 1 Score: 344.4 bits (882), Expect = 1.3e-91
Identity = 173/190 (91.05%), Postives = 178/190 (93.68%), Query Frame = 1 

Query: 1   MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60
           MEDDGG ELR++KEML IEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE
Sbjct: 25  MEDDGGYELRNSKEMLSIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 84

Query: 61  ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSG-----GGDGILIGFVDSGIYP 120
           ANGVKLVELDRGV+KMTTYTPEFLGLVKNNNDYKYNYSG     GGDGILIGFVDSGIYP
Sbjct: 85  ANGVKLVELDRGVKKMTTYTPEFLGLVKNNNDYKYNYSGGISGAGGDGILIGFVDSGIYP 144

Query: 121 THPSFSNNFGKEDDD--ELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLS 180
            HPSFSNNFGK+DDD  ELVCEEGPLFPKGCCNGKIVSA FFSAGAQAAAVLNSSIDFLS
Sbjct: 145 KHPSFSNNFGKDDDDYGELVCEEGPLFPKGCCNGKIVSAMFFSAGAQAAAVLNSSIDFLS 204

Query: 181 PFDAEGHGRY 184
           PFDAEGHG +
Sbjct: 205 PFDAEGHGSH 214

[Close]
BLAST of CSPI01G34220 vs. NCBI nr
Match: gi|703108927|ref|XP_010099159.1| (Subtilisin-like protease [Morus notabilis])

HSP 1 Score: 193.4 bits (490), Expect = 3.7e-46
Identity = 100/171 (58.48%), Postives = 124/171 (72.51%), Query Frame = 1 

Query: 20  DSHKKVLENSIK--NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRKMT 79
           +SH K+L+++++  +Y KLHSFK I+NGFAVHTTPS+ASKLR+  GV+LVE DRG + MT
Sbjct: 71  NSHDKLLQSTLETGSYTKLHSFKHIVNGFAVHTTPSQASKLRDVEGVRLVEKDRGAKLMT 130

Query: 80  TYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSFSNNFGKEDDDEL----- 139
           TYTP+FLGL +     +      G+GI+IGFVD+GI PTHPSF+ N        L     
Sbjct: 131 TYTPKFLGLREGVWAQEGGERNAGEGIVIGFVDTGINPTHPSFAFNPFNPFTSNLSRFSG 190

Query: 140 VCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
            CE GP FP   CNGKIVSARFFSAGAQAA  LN+S+DFLSP+DA GHG +
Sbjct: 191 ACEAGPHFPASSCNGKIVSARFFSAGAQAATTLNTSVDFLSPYDAVGHGSH 241

[Close]
BLAST of CSPI01G34220 vs. NCBI nr
Match: gi|657984334|ref|XP_008384255.1| (PREDICTED: subtilisin-like protease [Malus domestica])

HSP 1 Score: 191.4 bits (485), Expect = 1.4e-45
Identity = 102/176 (57.95%), Postives = 127/176 (72.16%), Query Frame = 1 

Query: 20  DSHKKVLENSIK--NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRKMT 79
           DSH ++L+++++  +Y KL+SFK I+NGFAVHTTPS+A KL+ A GVKLVE DRG + MT
Sbjct: 74  DSHDQLLQSALETGSYSKLYSFKHIVNGFAVHTTPSQAEKLKAAAGVKLVERDRGAKLMT 133

Query: 80  TYTPEFLGLVKNNNDYKYNYSGG-----GDGILIGFVDSGIYPTHPSFSNN-----FGKE 139
           TYTPEFLGL +      + + GG     G+GI+IGFVDSGI PTHPSF+N+      G  
Sbjct: 134 TYTPEFLGLPQG----AWTHDGGDDRSAGEGIVIGFVDSGINPTHPSFANDPLHPFTGNI 193

Query: 140 DDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
                 CE GP FP   CNGKIVSA+FFSAGA+ AA LNS++DFLSPFDA GHG +
Sbjct: 194 SHFLGSCETGPRFPASSCNGKIVSAKFFSAGAETAATLNSTVDFLSPFDAVGHGSH 245

The following BLAST results are available for this feature:
BLAST of CSPI01G34220 vs. Swiss-Prot
Match NameE-valueIdentityDescription
SBT24_ARATH2.0e-3949.46Subtilisin-like protease SBT2.4 OS=Arabidopsis thaliana GN=SBT2.4 PE=1 SV=1[more]
SBT26_ARATH2.7e-3345.22Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana GN=SBT2.6 PE=2 SV=1[more]
SBT25_ARATH1.8e-3245.51Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana GN=SBT2.5 PE=2 SV=1[more]
SBT22_ARATH7.5e-3141.95Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana GN=SBT2.2 PE=2 SV=1[more]
SBT23_ARATH5.3e-2939.31Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana GN=SBT2.3 PE=2 SV=1[more]
BLAST of CSPI01G34220 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LYI5_CUCSA6.9e-10098.91Uncharacterized protein OS=Cucumis sativus GN=Csa_1G701240 PE=4 SV=1[more]
W9RY25_9ROSA2.6e-4658.48Subtilisin-like protease OS=Morus notabilis GN=L484_014157 PE=3 SV=1[more]
B9HZB1_POPTR4.8e-4557.89Abnormal leaf shape family protein (Fragment) OS=Populus trichocarpa GN=POPTR_00... [more]
I1KNH1_SOYBN1.6e-4355.49Uncharacterized protein OS=Glycine max GN=GLYMA_07G263500 PE=3 SV=2[more]
A0A0B2RV96_GLYSO1.6e-4355.49Subtilisin-like protease (Fragment) OS=Glycine soja GN=glysoja_039498 PE=3 SV=1[more]
BLAST of CSPI01G34220 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G62340.11.1e-4049.46 PA-domain containing subtilase family protein[more]
AT4G30020.11.5e-3445.22 PA-domain containing subtilase family protein[more]
AT2G19170.11.0e-3345.51 subtilisin-like serine protease 3[more]
AT4G20430.14.2e-3241.95 Subtilase family protein[more]
AT5G44530.13.0e-3039.31 Subtilase family protein[more]
BLAST of CSPI01G34220 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778665448|ref|XP_004145469.2|9.9e-10098.91PREDICTED: subtilisin-like protease SBT2.4 [Cucumis sativus][more]
gi|700211752|gb|KGN66848.1|9.9e-10098.91hypothetical protein Csa_1G701240 [Cucumis sativus][more]
gi|659118464|ref|XP_008459133.1|1.3e-9191.05PREDICTED: LOW QUALITY PROTEIN: cucumisin, partial [Cucumis melo][more]
gi|703108927|ref|XP_010099159.1|3.7e-4658.48Subtilisin-like protease [Morus notabilis][more]
gi|657984334|ref|XP_008384255.1|1.4e-4557.95PREDICTED: subtilisin-like protease [Malus domestica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000209Peptidase_S8/S53_dom
IPR010259S8pro/Inhibitor_I9
IPR015500Peptidase_S8_subtilisin-rel
IPR023827Peptidase_S8_Asp-AS
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G34220.1CSPI01G34220.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 93..181
score: 4.
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 58..122
score: 5.89E-11coord: 160..181
score: 5.89
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9GENE3DG3DSA:3.30.70.80coord: 34..77
score: 2.
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 17..74
score: 8.7
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 1..183
score: 1.3
IPR023827Peptidase S8, subtilisin, Asp-active sitePROSITEPS00136SUBTILASE_ASPcoord: 106..117
scor
NoneNo IPR availablePANTHERPTHR10795:SF390SUBTILISIN-LIKE SERINE PROTEASEcoord: 1..183
score: 1.3