CmaCh18G002870 (gene) Cucurbita maxima (Rimu)

NameCmaCh18G002870
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionGDSL esterase/lipase
LocationCma_Chr18 : 1526887 .. 1528710 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTCCTCTTACAATGACGAAACGTCGTCCCGCCACCGCCACTTTGGTCCTCGTTCTCGTCCTGTTCGAATGTTCGAAGCAGATGGCCTTAGCGGAAAATGGGGTCCCGGCGATCTTCGTGTTCGGAGACTCTCTTGTGGACGTTGGCAACAACAATTTCCTTCATTCAGCTGCTAGAGCTAACTTTTACCCTTATGGGATTGATTTTCGTGGTGGTCCGACCGGGAGATTCTCTAATGGAAGAACTGTTATAGATATGATTGGTAAGTCTAATTATTTGATTATTGATTAATGATGGCAATTAAGGGTCTTAATGATTGATGTGTTCTTGATTCGTGGTGATTTAACTTGTAGTTGACTACCTTGGAATTCCGAACGCTCAAGAGTTTGCAAATCCCGATACTTCGGGAGATAGAATCCTTAAAGGTGTTAATTACGCTTCGGCGGCTGCTGGTATCCTCGACGAGACCGGTAGACACTATGTACGTTTTCATGGACTTTGTGTTTGTTAAATTAGGGATGTTCGTGAGTTGTATTAGGTTGGGTTGGGTTGGGTTGGTGTCTTTTTTAGACCAACCAAATTGTTCTGATTGTAAATTTATGTTGTGTGAGTACAGGGAGAGAGGTATAGTTTGAATCAGCAAGTGGTGAACTTCGAAAGCACTCGGAATGATTTAAGAAAATCAATGGACAATTGGAATTTGACTCGCTACTTATCGAGATCCATAACTTTCATGGCGTTTGGAAGCAATGATTACATAAACAACTACTTGATGCCCAATCTCTATACTTCTAGATTCAGATACACCTCCGACCAGTTCGCCGACCATCTTCTCAATAGCTATACTCGACAGCTTCTCGTGAGTCGTTTAACGAATCGGTTTAGAGTGAAAAGTCATTCCAAACATGCCTTAATTGTTCGTTTTTTAAATTTTCACAACAAACCCAAGTATGATTTTTACTAAGAAATGTCGAAACTGCATGCATGCACAGGCACTACGAAGTGCAGGGCTGAGGAAGTTTGTGGTGGCCGGAATTGGGCCGTTGGGTTGCATTCCGAACCAACGGGCGACTGGTGTGGCGCTTCCAGGGAGGTGTGTGGACAACGTGAATGAGATGCTGGGAGATTTTAACGAAGGGTTGAGATCGCTAGTAACTCAGCTGAATAGCCAGTACCCGGATTCGTACTTCGTGTACACCAATATCTATGGCATTTTTGGTGACATCTTGAACCACCCAGATACCTACGGTGAGTGATAGAAATAATGAACCTCTATAATGGTATGATATTGTCTACTTTTAGTATAAGTTTTCGTGACTTTATGGTTAAATGTGTGGTAGGGTTTCGAGTGGTGGACACCGCGTGCTGTGGAGTTGGGTTAAACAGAGGCCAAATAACGTGCCTACCACTCCAATTTCCATGCCTTAACCGAAATGAGTATGCGTTTTGGGATGCCTATCACCCAACTGAGGCTGCCTCTGTCGTGCTTGCCGACCGAGCTTTCAGAGGTCCACCCTCTGATTCCTACCCCATCAATATCCAACAGTTGGCTCTCCTATCCTTGTAATCGCGCACCACAGCCACCTCTCTTTCTACTGTAATGTTTGTGCTACTCAAGTCGATAAAGACACCATGTATGAATATGAATATGAATATGAACGTATAAACGTTCTAAGTTTCTGAACTAAATTTCAGGGACTAAATTTTAATACTTTAGAAGTATATACTAATGTAAAAAAATAACTTAAATTCAATCTAATCGATATTGAATCTAGTTCTTATCCATTATCATTTGCTCATTTCCATAGAAAGCAACATGGG

mRNA sequence

ATGTTTCCTCTTACAATGACGAAACGTCGTCCCGCCACCGCCACTTTGGTCCTCGTTCTCGTCCTGTTCGAATGTTCGAAGCAGATGGCCTTAGCGGAAAATGGGGTCCCGGCGATCTTCGTGTTCGGAGACTCTCTTGTGGACGTTGGCAACAACAATTTCCTTCATTCAGCTGCTAGAGCTAACTTTTACCCTTATGGGATTGATTTTCGTGGTGGTCCGACCGGGAGATTCTCTAATGGAAGAACTGTTATAGATATGATTGTTGACTACCTTGGAATTCCGAACGCTCAAGAGTTTGCAAATCCCGATACTTCGGGAGATAGAATCCTTAAAGGTGTTAATTACGCTTCGGCGGCTGCTGGTATCCTCGACGAGACCGGTAGACACTATGGAGAGAGGTATAGTTTGAATCAGCAAGTGGTGAACTTCGAAAGCACTCGGAATGATTTAAGAAAATCAATGGACAATTGGAATTTGACTCGCTACTTATCGAGATCCATAACTTTCATGGCGTTTGGAAGCAATGATTACATAAACAACTACTTGATGCCCAATCTCTATACTTCTAGATTCAGATACACCTCCGACCAGTTCGCCGACCATCTTCTCAATAGCTATACTCGACAGCTTCTCGCACTACGAAGTGCAGGGCTGAGGAAGTTTGTGGTGGCCGGAATTGGGCCGTTGGGTTGCATTCCGAACCAACGGGCGACTGGTGTGGCGCTTCCAGGGAGGTGTGTGGACAACGTGAATGAGATGCTGGGAGATTTTAACGAAGGGTTGAGATCGCTAGTAACTCAGCTGAATAGCCAGTACCCGGATTCGTACTTCGTGTACACCAATATCTATGGCATTTTTGGTGACATCTTGAACCACCCAGATACCTACGGGTTTCGAGTGGTGGACACCGCGTGCTGTGGAGTTGGGTTAAACAGAGGCCAAATAACGTGCCTACCACTCCAATTTCCATGCCTTAACCGAAATGAGTATGCGTTTTGGGATGCCTATCACCCAACTGAGGCTGCCTCTGTCGTGCTTGCCGACCGAGCTTTCAGAGGTCCACCCTCTGATTCCTACCCCATCAATATCCAACAGTTGGCTCTCCTATCCTTGTAATCGCGCACCACAGCCACCTCTCTTTCTACTGTAATGTTTGTGCTACTCAAGTCGATAAAGACACCATGTATGAATATGAATATGAATATGAACGTATAAACGTTCTAAGTTTCTGAACTAAATTTCAGGGACTAAATTTTAATACTTTAGAAGTATATACTAATGTAAAAAAATAACTTAAATTCAATCTAATCGATATTGAATCTAGTTCTTATCCATTATCATTTGCTCATTTCCATAGAAAGCAACATGGG

Coding sequence (CDS)

ATGTTTCCTCTTACAATGACGAAACGTCGTCCCGCCACCGCCACTTTGGTCCTCGTTCTCGTCCTGTTCGAATGTTCGAAGCAGATGGCCTTAGCGGAAAATGGGGTCCCGGCGATCTTCGTGTTCGGAGACTCTCTTGTGGACGTTGGCAACAACAATTTCCTTCATTCAGCTGCTAGAGCTAACTTTTACCCTTATGGGATTGATTTTCGTGGTGGTCCGACCGGGAGATTCTCTAATGGAAGAACTGTTATAGATATGATTGTTGACTACCTTGGAATTCCGAACGCTCAAGAGTTTGCAAATCCCGATACTTCGGGAGATAGAATCCTTAAAGGTGTTAATTACGCTTCGGCGGCTGCTGGTATCCTCGACGAGACCGGTAGACACTATGGAGAGAGGTATAGTTTGAATCAGCAAGTGGTGAACTTCGAAAGCACTCGGAATGATTTAAGAAAATCAATGGACAATTGGAATTTGACTCGCTACTTATCGAGATCCATAACTTTCATGGCGTTTGGAAGCAATGATTACATAAACAACTACTTGATGCCCAATCTCTATACTTCTAGATTCAGATACACCTCCGACCAGTTCGCCGACCATCTTCTCAATAGCTATACTCGACAGCTTCTCGCACTACGAAGTGCAGGGCTGAGGAAGTTTGTGGTGGCCGGAATTGGGCCGTTGGGTTGCATTCCGAACCAACGGGCGACTGGTGTGGCGCTTCCAGGGAGGTGTGTGGACAACGTGAATGAGATGCTGGGAGATTTTAACGAAGGGTTGAGATCGCTAGTAACTCAGCTGAATAGCCAGTACCCGGATTCGTACTTCGTGTACACCAATATCTATGGCATTTTTGGTGACATCTTGAACCACCCAGATACCTACGGGTTTCGAGTGGTGGACACCGCGTGCTGTGGAGTTGGGTTAAACAGAGGCCAAATAACGTGCCTACCACTCCAATTTCCATGCCTTAACCGAAATGAGTATGCGTTTTGGGATGCCTATCACCCAACTGAGGCTGCCTCTGTCGTGCTTGCCGACCGAGCTTTCAGAGGTCCACCCTCTGATTCCTACCCCATCAATATCCAACAGTTGGCTCTCCTATCCTTGTAA

Protein sequence

MFPLTMTKRRPATATLVLVLVLFECSKQMALAENGVPAIFVFGDSLVDVGNNNFLHSAARANFYPYGIDFRGGPTGRFSNGRTVIDMIVDYLGIPNAQEFANPDTSGDRILKGVNYASAAAGILDETGRHYGERYSLNQQVVNFESTRNDLRKSMDNWNLTRYLSRSITFMAFGSNDYINNYLMPNLYTSRFRYTSDQFADHLLNSYTRQLLALRSAGLRKFVVAGIGPLGCIPNQRATGVALPGRCVDNVNEMLGDFNEGLRSLVTQLNSQYPDSYFVYTNIYGIFGDILNHPDTYGFRVVDTACCGVGLNRGQITCLPLQFPCLNRNEYAFWDAYHPTEAASVVLADRAFRGPPSDSYPINIQQLALLSL
BLAST of CmaCh18G002870 vs. Swiss-Prot
Match: GDL28_ARATH (GDSL esterase/lipase At1g71250 OS=Arabidopsis thaliana GN=At1g71250 PE=2 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 6.0e-125
Identity = 214/355 (60.28%), Postives = 263/355 (74.08%), Query Frame = 1

Query: 16  LVLVLVLFECSKQMALAENGVPAIFVFGDSLVDVGNNNFLHSAARANFYPYGIDFRGGPT 75
           L L + +     ++   +  VPA+FV GDSLVD GNNNFL + ARANF PYGID    PT
Sbjct: 19  LALTVSVILQQPELVTGQARVPAMFVLGDSLVDAGNNNFLQTVARANFLPYGIDMNYQPT 78

Query: 76  GRFSNGRTVIDMIVDYLGIPNAQEFANPDTSGDRILKGVNYASAAAGILDETGRHYGERY 135
           GRFSNG T ID++   L IP+   FA+P TSG+RIL+GVNYASAAAGILD +G +YG R+
Sbjct: 79  GRFSNGLTFIDLLARLLEIPSPPPFADPTTSGNRILQGVNYASAAAGILDVSGYNYGGRF 138

Query: 136 SLNQQVVNFESTRNDLRKSMDNWNLTRYLSRSITFMAFGSNDYINNYLMPNLYTSRFRYT 195
           SLNQQ+VN E+T + LR  M   N T YL+RS+  + FGSNDYINNYLMPNLY S  R+ 
Sbjct: 139 SLNQQMVNLETTLSQLRTMMSPQNFTDYLARSLVVLVFGSNDYINNYLMPNLYDSSIRFR 198

Query: 196 SDQFADHLLNSYTRQLLALRSAGLRKFVVAGIGPLGCIPNQRATGVALPGRCVDNVNEML 255
              FA+ LL+ Y RQLL L S GLRK  + G+ PLGCIPNQRA G++ P RCVD+VN++L
Sbjct: 199 PPDFANLLLSQYARQLLTLYSLGLRKIFIPGVAPLGCIPNQRARGISPPDRCVDSVNQIL 258

Query: 256 GDFNEGLRSLVTQLNSQYPDSYFVYTNIYGIFGDILNHPDTYGFRVVDTACCGVGLNRGQ 315
           G FN+GL+SLV QLN + P + +VY N Y   GDILN+P  YGF VVD ACCG+G N+GQ
Sbjct: 259 GTFNQGLKSLVDQLNQRSPGAIYVYGNTYSAIGDILNNPAAYGFSVVDRACCGIGRNQGQ 318

Query: 316 ITCLPLQFPCLNRNEYAFWDAYHPTEAASVVLADRAFRGPPSDSYPINIQQLALL 371
           ITCLPLQ PC NRN+Y FWDA+HPT+ A+ +LA RAF GPPSD+YP+N+QQ+ LL
Sbjct: 319 ITCLPLQTPCPNRNQYVFWDAFHPTQTANSILARRAFYGPPSDAYPVNVQQMTLL 373

BLAST of CmaCh18G002870 vs. Swiss-Prot
Match: GDL75_ARATH (GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana GN=At5g08460 PE=2 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 6.5e-103
Identity = 195/365 (53.42%), Postives = 247/365 (67.67%), Query Frame = 1

Query: 15  TLVLV---LVLFECSKQMALAENGV--PAIFVFGDSLVDVGNNNFLHSAARANFYPYGID 74
           TLVLV   LV+F  +     +E     PA+FVFGDSLVD GNNN L+S AR+N+ PYGID
Sbjct: 21  TLVLVPWFLVVFVLAGGEDSSETTAMFPAMFVFGDSLVDNGNNNHLNSLARSNYLPYGID 80

Query: 75  FRGG-PTGRFSNGRTVIDMIVDYLGIPNAQEFANPDTSGDRILKGVNYASAAAGILDETG 134
           F G  PTGRFSNG+T++D I + LG+P    F +    G  IL GVNYASAA GIL+ETG
Sbjct: 81  FAGNQPTGRFSNGKTIVDFIGELLGLPEIPAFMDTVDGGVDILHGVNYASAAGGILEETG 140

Query: 135 RHYGERYSLNQQVVNFESTRNDLRKSMDNWNLTRYLSRSITFMAFGSNDYINNYLMPNLY 194
           RH GER+S+ +QV NFE T  ++ +SM   ++  Y+++S+  ++ G+NDYINNYL P L+
Sbjct: 141 RHLGERFSMGRQVENFEKTLMEISRSMRKESVKEYMAKSLVVVSLGNNDYINNYLKPRLF 200

Query: 195 TSRFRYTSDQFADHLLNSYTRQLLALRSAGLRKFVVAGIGPLGCIPNQRATGVALPGRCV 254
            S   Y    FAD LL+++T  LL L   G RKFV+AG+GPLGCIP+Q A   ALPG CV
Sbjct: 201 LSSSIYDPTSFADLLLSNFTTHLLELYGKGFRKFVIAGVGPLGCIPDQLAAQAALPGECV 260

Query: 255 DNVNEMLGDFNEGLRSLVTQLNSQ---YPDSYFVYTNIYGIFGDILNHPDTYGFRVVDTA 314
           + VNEM   FN  L SLV +LNS      ++ FVY N YG   DIL +P  YGF V D  
Sbjct: 261 EAVNEMAELFNNRLVSLVDRLNSDNKTASEAIFVYGNTYGAAVDILTNPFNYGFEVTDRG 320

Query: 315 CCGVGLNRGQITCLPLQFPCLNRNEYAFWDAYHPTEAASVVLADRAFRGPPSDSYPINIQ 371
           CCGVG NRG+ITCLPL  PC  R+ + FWDA+HPT+A ++++A RAF G  SD YPIN+ 
Sbjct: 321 CCGVGRNRGEITCLPLAVPCAFRDRHVFWDAFHPTQAFNLIIALRAFNGSKSDCYPINLS 380

BLAST of CmaCh18G002870 vs. Swiss-Prot
Match: GDL15_ARATH (GDSL esterase/lipase At1g29670 OS=Arabidopsis thaliana GN=At1g29670 PE=2 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 2.2e-95
Identity = 184/357 (51.54%), Postives = 242/357 (67.79%), Query Frame = 1

Query: 16  LVLVLVLFECSKQMALAENGVPAIFVFGDSLVDVGNNNFLHSAARANFYPYGIDFRGGPT 75
           +VLVL+ F  S   A A+  VP  FVFGDSLVD GNNN L S AR+N++PYGIDF GGPT
Sbjct: 10  VVLVLLCFGFSVVKAQAQAQVPCFFVFGDSLVDNGNNNGLISIARSNYFPYGIDF-GGPT 69

Query: 76  GRFSNGRTVIDMIVDYLGIPNAQEFANPDTSGDRILKGVNYASAAAGILDETGRHYGERY 135
           GRFSNG+T +D+I + LG  N    A    SG +IL GVNYASAAAGI +ETGR  G+R 
Sbjct: 70  GRFSNGKTTVDVIAELLGF-NGYIPAYNTVSGRQILSGVNYASAAAGIREETGRQLGQRI 129

Query: 136 SLNQQVVNFESTRNDLRKSM-DNWNLTRYLSRSITFMAFGSNDYINNYLMPNLYTSRFRY 195
           S + QV N+++T + + + + D      YL R I  +  GSNDY+NNY MP  Y+S  ++
Sbjct: 130 SFSGQVRNYQTTVSQVVQLLGDETRAADYLKRCIYSVGLGSNDYLNNYFMPTFYSSSRQF 189

Query: 196 TSDQFADHLLNSYTRQLLALRSAGLRKFVVAGIGPLGCIPNQRATGVALPGRCVDNVNEM 255
           T +Q+A+ L++ Y+ QL AL + G RKF ++GIG +GC PN  A G      CVD +N  
Sbjct: 190 TPEQYANDLISRYSTQLNALYNYGARKFALSGIGAVGCSPNALA-GSPDGRTCVDRINSA 249

Query: 256 LGDFNEGLRSLVTQLNSQYPDSYFVYTNIYGIFGDILNHPDTYGFRVVDTACCGVGLNRG 315
              FN  LRSLV QLN+ +PD+ F+Y N YGIF D++ +P  +GFRV +  CCG+G N G
Sbjct: 250 NQIFNNKLRSLVDQLNNNHPDAKFIYINAYGIFQDMITNPARFGFRVTNAGCCGIGRNAG 309

Query: 316 QITCLPLQFPCLNRNEYAFWDAYHPTEAASVVLADRAFRG-PPSDSYPINIQQLALL 371
           QITCLP Q PC +RN Y FWDA+HPTEAA+V++A R++     SD+YP++I +LA L
Sbjct: 310 QITCLPGQRPCRDRNAYVFWDAFHPTEAANVIIARRSYNAQSASDAYPMDISRLAQL 363

BLAST of CmaCh18G002870 vs. Swiss-Prot
Match: GDL29_ARATH (GDSL esterase/lipase At1g71691 OS=Arabidopsis thaliana GN=At1g71691 PE=2 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 6.1e-93
Identity = 167/337 (49.55%), Postives = 236/337 (70.03%), Query Frame = 1

Query: 36  VPAIFVFGDSLVDVGNNNFLHSAARANFYPYGIDFRGGPTGRFSNGRTVIDMIVDYLGIP 95
           VPA+FVFGDSL+D GNNN + S A+AN++PYGIDF GGPTGRF NG T++D I   LG+P
Sbjct: 53  VPALFVFGDSLIDNGNNNNIPSFAKANYFPYGIDFNGGPTGRFCNGLTMVDGIAQLLGLP 112

Query: 96  NAQEFANPDTSGDRILKGVNYASAAAGILDETGRHYGERYSLNQQVVNFESTRNDLR-KS 155
               ++  + +GD++L+GVNYASAAAGIL +TG ++  R   +QQ+ NFE+T + +  KS
Sbjct: 113 LIPAYS--EATGDQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFETTLDQVASKS 172

Query: 156 MDNWNLTRYLSRSITFMAFGSNDYINNYLMPNLYTSRFRYTSDQFADHLLNSYTRQLLAL 215
                +   ++RS+ F+  GSNDY+NNYLMPN + +R +Y S QF D L+  YT QL  L
Sbjct: 173 GGAVAIADSVTRSLFFIGMGSNDYLNNYLMPN-FPTRNQYNSQQFGDLLVQHYTDQLTRL 232

Query: 216 RSAGLRKFVVAGIGPLGCIPNQRATGVALPGRCVDNVNEMLGDFNEGLRSLVTQLNSQYP 275
            + G RKFVVAG+G +GCIP+  A G    G+C + VN+++  FN  ++++++ LN   P
Sbjct: 233 YNLGGRKFVVAGLGRMGCIPSILAQGN--DGKCSEEVNQLVLPFNTNVKTMISNLNQNLP 292

Query: 276 DSYFVYTNIYGIFGDILNHPDTYGFRVVDTACCGVGLNRGQITCLPLQFPCLNRNEYAFW 335
           D+ F+Y +I  +F DI+ +   YG   +D  CCG+G NRGQITCLP + PC NR++Y FW
Sbjct: 293 DAKFIYLDIAHMFEDIVANQAAYGLTTMDKGCCGIGKNRGQITCLPFETPCPNRDQYVFW 352

Query: 336 DAYHPTEAASVVLADRAFRGPPSDSYPINIQQLALLS 372
           DA+HPTE  ++++A +AF G  + +YPINIQQLA L+
Sbjct: 353 DAFHPTEKVNLIMAKKAFAGDRTVAYPINIQQLASLN 384

BLAST of CmaCh18G002870 vs. Swiss-Prot
Match: GLIP7_ARATH (GDSL esterase/lipase 7 OS=Arabidopsis thaliana GN=GLIP7 PE=2 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 8.8e-92
Identity = 172/338 (50.89%), Postives = 227/338 (67.16%), Query Frame = 1

Query: 37  PAIFVFGDSLVDVGNNNFLHSAARANFYPYGIDFRGGPTGRFSNGRTVIDMIVDYLGIPN 96
           PA FVFGDSLVD GNNN++ + ARAN++PYGIDF G PTGRF NGRTV+D    YLG+P 
Sbjct: 29  PAFFVFGDSLVDSGNNNYIPTLARANYFPYGIDF-GFPTGRFCNGRTVVDYGATYLGLPL 88

Query: 97  AQEFANPDTSGDRILKGVNYASAAAGILDETGRHYGERYSLNQQVVNFESTRN-DLRKSM 156
              + +P + G   L+GVNYASAAAGILDETGRHYG R + N Q+  FE T    LR+  
Sbjct: 89  VPPYLSPLSIGQNALRGVNYASAAAGILDETGRHYGARTTFNGQISQFEITIELRLRRFF 148

Query: 157 DN-WNLTRYLSRSITFMAFGSNDYINNYLMPNLYTSRFRYTSDQFADHLLNSYTRQLLAL 216
            N  +L +YL++SI  +  GSNDYINNYLMP  Y++   Y+ + +AD L+ + + Q+  L
Sbjct: 149 QNPADLRKYLAKSIIGINIGSNDYINNYLMPERYSTSQTYSGEDYADLLIKTLSAQISRL 208

Query: 217 RSAGLRKFVVAGIGPLGCIPNQRA--TGVALPGRCVDNVNEMLGDFNEGLRSLVTQLNSQ 276
            + G RK V+AG GPLGCIP+Q +  TG    G CV  +N M+  FN  L+ L   LN+ 
Sbjct: 209 YNLGARKMVLAGSGPLGCIPSQLSMVTGNNTSG-CVTKINNMVSMFNSRLKDLANTLNTT 268

Query: 277 YPDSYFVYTNIYGIFGDILNHPDTYGFRVVDTACCGVGLNRGQITCLPLQFPCLNRNEYA 336
            P S+FVY N++ +F D++ +P  YG  V + ACCG G   G +TCLPLQ PCL+RN+Y 
Sbjct: 269 LPGSFFVYQNVFDLFHDMVVNPSRYGLVVSNEACCGNGRYGGALTCLPLQQPCLDRNQYV 328

Query: 337 FWDAYHPTEAASVVLADRAFRGPPSDSYPINIQQLALL 371
           FWDA+HPTE A+ ++A   F    + SYPI++ +LA L
Sbjct: 329 FWDAFHPTETANKIIAHNTFSKSANYSYPISVYELAKL 364

BLAST of CmaCh18G002870 vs. TrEMBL
Match: A0A0A0LUJ9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G056930 PE=4 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 1.5e-175
Identity = 297/359 (82.73%), Postives = 326/359 (90.81%), Query Frame = 1

Query: 12  ATATLVLVLVLFECSKQMALAENGVPAIFVFGDSLVDVGNNNFLHSAARANFYPYGIDFR 71
           A A  V+VLV  + S QM  AENGV AIFVFGDSLVDVGNNNFLHSAARAN+YPYG+DF 
Sbjct: 2   AAAAGVVVLVFLKSSYQMVEAENGVSAIFVFGDSLVDVGNNNFLHSAARANYYPYGVDFT 61

Query: 72  GGPTGRFSNGRTVIDMIVDYLGIPNAQEFANPDTSGDRILKGVNYASAAAGILDETGRHY 131
            GPTGRFSNGRTVIDM VD LGIPNA EF+NPDTSGDRIL GVNYASAAAGILDETGRHY
Sbjct: 62  DGPTGRFSNGRTVIDMFVDMLGIPNAPEFSNPDTSGDRILNGVNYASAAAGILDETGRHY 121

Query: 132 GERYSLNQQVVNFESTRNDLRKSMDNWNLTRYLSRSITFMAFGSNDYINNYLMPNLYTSR 191
           G+RY+L+QQVVNFEST NDLR+SM +WNLTRYLS+SI F+AFGSNDYINNYLMPNLYT+R
Sbjct: 122 GDRYTLSQQVVNFESTLNDLRRSMGSWNLTRYLSKSIAFLAFGSNDYINNYLMPNLYTTR 181

Query: 192 FRYTSDQFADHLLNSYTRQLLALRSAGLRKFVVAGIGPLGCIPNQRATGVALPGRCVDNV 251
           FRY S+QFA+ LLN Y+RQLLAL+S G++K V+AG+GPLGCIPNQRATGV LPGRC D V
Sbjct: 182 FRYNSNQFANLLLNRYSRQLLALQSVGVKKLVIAGLGPLGCIPNQRATGVTLPGRCADKV 241

Query: 252 NEMLGDFNEGLRSLVTQLNSQYPDSYFVYTNIYGIFGDILNHPDTYGFRVVDTACCGVGL 311
           NEMLG FNEGL+SLVTQLNSQYPD+ FVYTNIYGIFGDILN+P+TYGF VVDTACCGVGL
Sbjct: 242 NEMLGAFNEGLKSLVTQLNSQYPDTKFVYTNIYGIFGDILNNPETYGFSVVDTACCGVGL 301

Query: 312 NRGQITCLPLQFPCLNRNEYAFWDAYHPTEAASVVLADRAFRGPPSDSYPINIQQLALL 371
           NRGQITCLPLQFPCLNRNEY FWDA+HPTEAAS +LA RAFRGPPSDSYPIN+QQLAL+
Sbjct: 302 NRGQITCLPLQFPCLNRNEYVFWDAFHPTEAASYILAGRAFRGPPSDSYPINVQQLALI 360

BLAST of CmaCh18G002870 vs. TrEMBL
Match: F6GVY3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0089g00940 PE=4 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 9.3e-133
Identity = 230/369 (62.33%), Postives = 293/369 (79.40%), Query Frame = 1

Query: 4   LTMTKRRP--ATATLVLVLVLFECSKQMALAENGVPAIFVFGDSLVDVGNNNFLHSAARA 63
           +TM K++P  + A L+L+L+  + S Q+A ++  VPAIF FGDSL+D GNNNFL S A++
Sbjct: 3   ITMVKQQPLWSAAVLLLLLLSLQFSTQVARSQR-VPAIFCFGDSLIDDGNNNFLDSIAKS 62

Query: 64  NFYPYGIDFRGGPTGRFSNGRTVIDMIVDYLGIPNAQEFANPDTSGDRILKGVNYASAAA 123
           N+YPYGIDFRG PTGRF NG+T++D++ + LG+   Q FA+P ++G +I  GVNYASAAA
Sbjct: 63  NYYPYGIDFRG-PTGRFCNGKTIVDLLAEMLGVSYPQPFADPGSTGSKIFSGVNYASAAA 122

Query: 124 GILDETGRHYGERYSLNQQVVNFESTRNDLRKSMDNWNLTRYLSRSITFMAFGSNDYINN 183
           GILDETG++YG+R+SL+QQV+NFE+T + +R   +   L+RYL++SI  M FGSNDY+NN
Sbjct: 123 GILDETGQNYGQRFSLSQQVLNFETTLSQMRTMANGTTLSRYLAKSIVIMVFGSNDYLNN 182

Query: 184 YLMPNLYTSRFRYTSDQFADHLLNSYTRQLLALRSAGLRKFVVAGIGPLGCIPNQRATGV 243
           YLMP+LY S + Y+   FA+ LLN Y RQ+LAL S GLRKF +AGIGPLGC+PNQRA  +
Sbjct: 183 YLMPSLYPSSYNYSPPDFANLLLNHYARQILALYSLGLRKFFLAGIGPLGCMPNQRA--L 242

Query: 244 ALPGRCVDNVNEMLGDFNEGLRSLVTQLNSQYPDSYFVYTNIYGIFGDILNHPDTYGFRV 303
           A PGRC+D  N++LG FNEGLR+LV QLN  +P S FVY N YGIFGDILN+P TYGF V
Sbjct: 243 APPGRCLDYDNQILGTFNEGLRALVNQLNGNHPGSIFVYGNTYGIFGDILNNPATYGFSV 302

Query: 304 VDTACCGVGLNRGQITCLPLQFPCLNRNEYAFWDAYHPTEAASVVLADRAFRGPPSDSYP 363
           VD  CCG+G N+GQITCLP+Q PCLNRNEY FWDA+HPT AA+V+LA  AF GPPSD YP
Sbjct: 303 VDRGCCGLGRNQGQITCLPMQMPCLNRNEYVFWDAFHPTTAANVILAQTAFYGPPSDCYP 362

Query: 364 INIQQLALL 371
           IN+QQ+AL+
Sbjct: 363 INVQQMALI 367

BLAST of CmaCh18G002870 vs. TrEMBL
Match: M5VKG0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008473mg PE=4 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 4.6e-132
Identity = 221/332 (66.57%), Postives = 271/332 (81.63%), Query Frame = 1

Query: 39  IFVFGDSLVDVGNNNFLHSAARANFYPYGIDFRGGPTGRFSNGRTVIDMIVDYLGIPNAQ 98
           +F+FGDSL+DVGNNN+L S A++N+YPYG DFR GPTGRFSNGRTV+DM+ + LG+P   
Sbjct: 1   MFIFGDSLIDVGNNNYLSSLAKSNYYPYGCDFRAGPTGRFSNGRTVVDMLGNLLGLPYVP 60

Query: 99  EFANPDTSGDRILKGVNYASAAAGILDETGRHYGERYSLNQQVVNFESTRNDLRKSMDNW 158
            FA+P+T G +IL GVNYASAAAGILDETG+H+G+RYSL+QQV+NFEST + LR  M   
Sbjct: 61  PFADPNTKGTKILGGVNYASAAAGILDETGQHFGQRYSLSQQVLNFESTLDQLRPMMSGA 120

Query: 159 NLTRYLSRSITFMAFGSNDYINNYLMPNLYTSRFRYTSDQFADHLLNSYTRQLLALRSAG 218
           N+T+YL++SI  + FGSNDYINNYL+P+LY+S + YT   FA+ LLN YT+Q+LAL S G
Sbjct: 121 NMTQYLAKSIAVLEFGSNDYINNYLLPSLYSSSYIYTPPAFANLLLNRYTQQILALHSVG 180

Query: 219 LRKFVVAGIGPLGCIPNQRATGVALPGRCVDNVNEMLGDFNEGLRSLVTQLNSQYPDSYF 278
           L+KFV+AGIGPLGCIPNQRA  +A PGRCVD VN++LG FNEGLRSL  QLN+ +P + F
Sbjct: 181 LKKFVLAGIGPLGCIPNQRA--LAQPGRCVDYVNQILGSFNEGLRSLANQLNTNHPGAIF 240

Query: 279 VYTNIYGIFGDILNHPDTYGFRVVDTACCGVGLNRGQITCLPLQFPCLNRNEYAFWDAYH 338
           VY N Y  FGD+LN+P  YGF V+D  CCG+G +RGQITCLP   PC NRN+Y FWDAYH
Sbjct: 241 VYANTYAAFGDMLNNPALYGFSVIDRGCCGIGRDRGQITCLPYSVPCANRNQYIFWDAYH 300

Query: 339 PTEAASVVLADRAFRGPPSDSYPINIQQLALL 371
           PTEAA+ VLA RAF GPPSD YPIN+QQ+AL+
Sbjct: 301 PTEAANAVLAWRAFNGPPSDCYPINVQQMALI 330

BLAST of CmaCh18G002870 vs. TrEMBL
Match: K4D878_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 1.1e-128
Identity = 219/340 (64.41%), Postives = 271/340 (79.71%), Query Frame = 1

Query: 34  NGVP-AIFVFGDSLVDVGNNNFLHSAARANFYPYGIDFRGGPTGRFSNGRTVIDMIVDYL 93
           NG P A+++FGDSLVD GNNNFL++ A+AN++PYGIDF  GPTGRFSNG+T +D++ + L
Sbjct: 19  NGSPTALWIFGDSLVDNGNNNFLNTIAKANYFPYGIDFSRGPTGRFSNGKTFVDILGELL 78

Query: 94  GIPNAQEFANPDTSGDRILKGVNYASAAAGILDETGRHYGERYSLNQQVVNFESTRNDLR 153
           G+P+   FANP T G+RIL GVNYASAA GILDETG+HY +RY+L+QQV+NFEST + LR
Sbjct: 79  GVPSPPPFANPSTRGERILGGVNYASAAGGILDETGKHYMDRYTLSQQVINFESTLSQLR 138

Query: 154 KSMDNWNLTRYLSRSITFMAFGSNDYINNYLMPNLYTSRFRYTSDQFADHLLNSYTRQLL 213
             M   +L  YLSRSI  M FGSNDYINNYL+P+LY++   Y   QF++ LLN Y RQL+
Sbjct: 139 TMMSPGDLNTYLSRSIAVMVFGSNDYINNYLLPSLYSTSINYNPQQFSNLLLNHYARQLV 198

Query: 214 ALRSAGLRKFVVAGIGPLGCIPNQRATGVALPGRCVDNVNEMLGDFNEGLRSLVTQLNS- 273
           AL S GLRKF++AG+GPLGCIPNQ ATG A PGRCVD VN++LG FNEGLRSLVT+LN+ 
Sbjct: 199 ALYSVGLRKFLIAGVGPLGCIPNQLATGQAPPGRCVDYVNQILGSFNEGLRSLVTRLNNG 258

Query: 274 QYPDSYFVYTNIYGIFGDILNHPDTYGFRVVDTACCGVGLNRGQITCLPLQFPCLNRNEY 333
            +P + FVY N Y   GDILN+P  YGF V D ACCGVG N+GQITCLP QFPCL+R++Y
Sbjct: 259 SHPGAVFVYGNTYAAIGDILNNPARYGFNVWDRACCGVGRNQGQITCLPYQFPCLDRSKY 318

Query: 334 AFWDAYHPTEAASVVLADRAFRGPPSDSYPINIQQLALLS 372
            FWDA+HPT+A   +LA RA+ GPPSD YP+N+QQ+A L+
Sbjct: 319 IFWDAFHPTQAVDAILAQRAYYGPPSDCYPMNVQQMAALN 358

BLAST of CmaCh18G002870 vs. TrEMBL
Match: A0A061FQW3_THECC (GDSL-like Lipase/Acylhydrolase superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_036011 PE=4 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 1.4e-128
Identity = 223/356 (62.64%), Postives = 281/356 (78.93%), Query Frame = 1

Query: 16  LVLVLVLFECSKQMALAENGVPAIFVFGDSLVDVGNNNFLHSAARANFYPYGIDFRGGPT 75
           +  V+V+F+ S  +A +E   PA+FVFGDSLVDVGNNNFL S A++N++PYGIDF   PT
Sbjct: 12  ITFVIVIFQYSNGVAGSERA-PALFVFGDSLVDVGNNNFLSSIAKSNYFPYGIDFNMQPT 71

Query: 76  GRFSNGRTVIDMIVDYLGIPNAQEFANPDTSGDRILKGVNYASAAAGILDETGRHYGERY 135
           GRFSNG+T +D+I + LG+P    FA+P++ G +IL G+NYASAAAGILDETG+HYG RY
Sbjct: 72  GRFSNGKTFVDIIGEMLGVPYPPAFADPNSVGVKILGGMNYASAAAGILDETGQHYGARY 131

Query: 136 SLNQQVVNFESTRNDLRKSMDNWNLTRYLSRSITFMAFGSNDYINNYLMPNLYTSRFRYT 195
           SL++QVVNFEST + LR  M + N+T +L++SI  M FGSNDYINNYLMP++Y+S + Y+
Sbjct: 132 SLSRQVVNFESTLDQLRTMMGS-NVTNFLAKSIAIMVFGSNDYINNYLMPSIYSSSYTYS 191

Query: 196 SDQFADHLLNSYTRQLLALRSAGLRKFVVAGIGPLGCIPNQRATGVALPGRCVDNVNEML 255
             +FA+ LLN Y RQLLAL S GLRKF +AG+GPLGCIPNQRATG A PGRCVD VN++L
Sbjct: 192 PSEFANLLLNHYGRQLLALYSVGLRKFFIAGVGPLGCIPNQRATGQAAPGRCVDYVNQIL 251

Query: 256 GDFNEGLRSLVTQLNSQYPDSYFVYTNIYGIFGDILNHPDTYGFRVVDTACCGVGLNRGQ 315
           G FN+G++SLV QLN++ P + FVY N YG  GDILN P TYGF VVD  CCG+G N+GQ
Sbjct: 252 GTFNQGIKSLVEQLNNR-PGAIFVYGNTYGAVGDILNDPSTYGFSVVDKGCCGIGRNQGQ 311

Query: 316 ITCLPLQFPCLNRNEYAFWDAYHPTEAASVVLADRAFRGPPSDSYPINIQQLALLS 372
           ITCLP  +PC +R +Y FWDA+HPT+A + +LA RAF GPP DSYPINIQQ+ L +
Sbjct: 312 ITCLPFAYPCPDRTQYVFWDAFHPTQAVNSILARRAFYGPPLDSYPINIQQMTLFN 364

BLAST of CmaCh18G002870 vs. TAIR10
Match: AT1G71250.1 (AT1G71250.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 448.7 bits (1153), Expect = 3.4e-126
Identity = 214/355 (60.28%), Postives = 263/355 (74.08%), Query Frame = 1

Query: 16  LVLVLVLFECSKQMALAENGVPAIFVFGDSLVDVGNNNFLHSAARANFYPYGIDFRGGPT 75
           L L + +     ++   +  VPA+FV GDSLVD GNNNFL + ARANF PYGID    PT
Sbjct: 19  LALTVSVILQQPELVTGQARVPAMFVLGDSLVDAGNNNFLQTVARANFLPYGIDMNYQPT 78

Query: 76  GRFSNGRTVIDMIVDYLGIPNAQEFANPDTSGDRILKGVNYASAAAGILDETGRHYGERY 135
           GRFSNG T ID++   L IP+   FA+P TSG+RIL+GVNYASAAAGILD +G +YG R+
Sbjct: 79  GRFSNGLTFIDLLARLLEIPSPPPFADPTTSGNRILQGVNYASAAAGILDVSGYNYGGRF 138

Query: 136 SLNQQVVNFESTRNDLRKSMDNWNLTRYLSRSITFMAFGSNDYINNYLMPNLYTSRFRYT 195
           SLNQQ+VN E+T + LR  M   N T YL+RS+  + FGSNDYINNYLMPNLY S  R+ 
Sbjct: 139 SLNQQMVNLETTLSQLRTMMSPQNFTDYLARSLVVLVFGSNDYINNYLMPNLYDSSIRFR 198

Query: 196 SDQFADHLLNSYTRQLLALRSAGLRKFVVAGIGPLGCIPNQRATGVALPGRCVDNVNEML 255
              FA+ LL+ Y RQLL L S GLRK  + G+ PLGCIPNQRA G++ P RCVD+VN++L
Sbjct: 199 PPDFANLLLSQYARQLLTLYSLGLRKIFIPGVAPLGCIPNQRARGISPPDRCVDSVNQIL 258

Query: 256 GDFNEGLRSLVTQLNSQYPDSYFVYTNIYGIFGDILNHPDTYGFRVVDTACCGVGLNRGQ 315
           G FN+GL+SLV QLN + P + +VY N Y   GDILN+P  YGF VVD ACCG+G N+GQ
Sbjct: 259 GTFNQGLKSLVDQLNQRSPGAIYVYGNTYSAIGDILNNPAAYGFSVVDRACCGIGRNQGQ 318

Query: 316 ITCLPLQFPCLNRNEYAFWDAYHPTEAASVVLADRAFRGPPSDSYPINIQQLALL 371
           ITCLPLQ PC NRN+Y FWDA+HPT+ A+ +LA RAF GPPSD+YP+N+QQ+ LL
Sbjct: 319 ITCLPLQTPCPNRNQYVFWDAFHPTQTANSILARRAFYGPPSDAYPVNVQQMTLL 373

BLAST of CmaCh18G002870 vs. TAIR10
Match: AT5G08460.1 (AT5G08460.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 375.6 bits (963), Expect = 3.7e-104
Identity = 195/365 (53.42%), Postives = 247/365 (67.67%), Query Frame = 1

Query: 15  TLVLV---LVLFECSKQMALAENGV--PAIFVFGDSLVDVGNNNFLHSAARANFYPYGID 74
           TLVLV   LV+F  +     +E     PA+FVFGDSLVD GNNN L+S AR+N+ PYGID
Sbjct: 21  TLVLVPWFLVVFVLAGGEDSSETTAMFPAMFVFGDSLVDNGNNNHLNSLARSNYLPYGID 80

Query: 75  FRGG-PTGRFSNGRTVIDMIVDYLGIPNAQEFANPDTSGDRILKGVNYASAAAGILDETG 134
           F G  PTGRFSNG+T++D I + LG+P    F +    G  IL GVNYASAA GIL+ETG
Sbjct: 81  FAGNQPTGRFSNGKTIVDFIGELLGLPEIPAFMDTVDGGVDILHGVNYASAAGGILEETG 140

Query: 135 RHYGERYSLNQQVVNFESTRNDLRKSMDNWNLTRYLSRSITFMAFGSNDYINNYLMPNLY 194
           RH GER+S+ +QV NFE T  ++ +SM   ++  Y+++S+  ++ G+NDYINNYL P L+
Sbjct: 141 RHLGERFSMGRQVENFEKTLMEISRSMRKESVKEYMAKSLVVVSLGNNDYINNYLKPRLF 200

Query: 195 TSRFRYTSDQFADHLLNSYTRQLLALRSAGLRKFVVAGIGPLGCIPNQRATGVALPGRCV 254
            S   Y    FAD LL+++T  LL L   G RKFV+AG+GPLGCIP+Q A   ALPG CV
Sbjct: 201 LSSSIYDPTSFADLLLSNFTTHLLELYGKGFRKFVIAGVGPLGCIPDQLAAQAALPGECV 260

Query: 255 DNVNEMLGDFNEGLRSLVTQLNSQ---YPDSYFVYTNIYGIFGDILNHPDTYGFRVVDTA 314
           + VNEM   FN  L SLV +LNS      ++ FVY N YG   DIL +P  YGF V D  
Sbjct: 261 EAVNEMAELFNNRLVSLVDRLNSDNKTASEAIFVYGNTYGAAVDILTNPFNYGFEVTDRG 320

Query: 315 CCGVGLNRGQITCLPLQFPCLNRNEYAFWDAYHPTEAASVVLADRAFRGPPSDSYPINIQ 371
           CCGVG NRG+ITCLPL  PC  R+ + FWDA+HPT+A ++++A RAF G  SD YPIN+ 
Sbjct: 321 CCGVGRNRGEITCLPLAVPCAFRDRHVFWDAFHPTQAFNLIIALRAFNGSKSDCYPINLS 380

BLAST of CmaCh18G002870 vs. TAIR10
Match: AT1G29670.1 (AT1G29670.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 350.5 bits (898), Expect = 1.3e-96
Identity = 184/357 (51.54%), Postives = 242/357 (67.79%), Query Frame = 1

Query: 16  LVLVLVLFECSKQMALAENGVPAIFVFGDSLVDVGNNNFLHSAARANFYPYGIDFRGGPT 75
           +VLVL+ F  S   A A+  VP  FVFGDSLVD GNNN L S AR+N++PYGIDF GGPT
Sbjct: 10  VVLVLLCFGFSVVKAQAQAQVPCFFVFGDSLVDNGNNNGLISIARSNYFPYGIDF-GGPT 69

Query: 76  GRFSNGRTVIDMIVDYLGIPNAQEFANPDTSGDRILKGVNYASAAAGILDETGRHYGERY 135
           GRFSNG+T +D+I + LG  N    A    SG +IL GVNYASAAAGI +ETGR  G+R 
Sbjct: 70  GRFSNGKTTVDVIAELLGF-NGYIPAYNTVSGRQILSGVNYASAAAGIREETGRQLGQRI 129

Query: 136 SLNQQVVNFESTRNDLRKSM-DNWNLTRYLSRSITFMAFGSNDYINNYLMPNLYTSRFRY 195
           S + QV N+++T + + + + D      YL R I  +  GSNDY+NNY MP  Y+S  ++
Sbjct: 130 SFSGQVRNYQTTVSQVVQLLGDETRAADYLKRCIYSVGLGSNDYLNNYFMPTFYSSSRQF 189

Query: 196 TSDQFADHLLNSYTRQLLALRSAGLRKFVVAGIGPLGCIPNQRATGVALPGRCVDNVNEM 255
           T +Q+A+ L++ Y+ QL AL + G RKF ++GIG +GC PN  A G      CVD +N  
Sbjct: 190 TPEQYANDLISRYSTQLNALYNYGARKFALSGIGAVGCSPNALA-GSPDGRTCVDRINSA 249

Query: 256 LGDFNEGLRSLVTQLNSQYPDSYFVYTNIYGIFGDILNHPDTYGFRVVDTACCGVGLNRG 315
              FN  LRSLV QLN+ +PD+ F+Y N YGIF D++ +P  +GFRV +  CCG+G N G
Sbjct: 250 NQIFNNKLRSLVDQLNNNHPDAKFIYINAYGIFQDMITNPARFGFRVTNAGCCGIGRNAG 309

Query: 316 QITCLPLQFPCLNRNEYAFWDAYHPTEAASVVLADRAFRG-PPSDSYPINIQQLALL 371
           QITCLP Q PC +RN Y FWDA+HPTEAA+V++A R++     SD+YP++I +LA L
Sbjct: 310 QITCLPGQRPCRDRNAYVFWDAFHPTEAANVIIARRSYNAQSASDAYPMDISRLAQL 363

BLAST of CmaCh18G002870 vs. TAIR10
Match: AT1G71691.2 (AT1G71691.2 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 342.4 bits (877), Expect = 3.4e-94
Identity = 167/337 (49.55%), Postives = 236/337 (70.03%), Query Frame = 1

Query: 36  VPAIFVFGDSLVDVGNNNFLHSAARANFYPYGIDFRGGPTGRFSNGRTVIDMIVDYLGIP 95
           VPA+FVFGDSL+D GNNN + S A+AN++PYGIDF GGPTGRF NG T++D I   LG+P
Sbjct: 53  VPALFVFGDSLIDNGNNNNIPSFAKANYFPYGIDFNGGPTGRFCNGLTMVDGIAQLLGLP 112

Query: 96  NAQEFANPDTSGDRILKGVNYASAAAGILDETGRHYGERYSLNQQVVNFESTRNDLR-KS 155
               ++  + +GD++L+GVNYASAAAGIL +TG ++  R   +QQ+ NFE+T + +  KS
Sbjct: 113 LIPAYS--EATGDQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFETTLDQVASKS 172

Query: 156 MDNWNLTRYLSRSITFMAFGSNDYINNYLMPNLYTSRFRYTSDQFADHLLNSYTRQLLAL 215
                +   ++RS+ F+  GSNDY+NNYLMPN + +R +Y S QF D L+  YT QL  L
Sbjct: 173 GGAVAIADSVTRSLFFIGMGSNDYLNNYLMPN-FPTRNQYNSQQFGDLLVQHYTDQLTRL 232

Query: 216 RSAGLRKFVVAGIGPLGCIPNQRATGVALPGRCVDNVNEMLGDFNEGLRSLVTQLNSQYP 275
            + G RKFVVAG+G +GCIP+  A G    G+C + VN+++  FN  ++++++ LN   P
Sbjct: 233 YNLGGRKFVVAGLGRMGCIPSILAQGN--DGKCSEEVNQLVLPFNTNVKTMISNLNQNLP 292

Query: 276 DSYFVYTNIYGIFGDILNHPDTYGFRVVDTACCGVGLNRGQITCLPLQFPCLNRNEYAFW 335
           D+ F+Y +I  +F DI+ +   YG   +D  CCG+G NRGQITCLP + PC NR++Y FW
Sbjct: 293 DAKFIYLDIAHMFEDIVANQAAYGLTTMDKGCCGIGKNRGQITCLPFETPCPNRDQYVFW 352

Query: 336 DAYHPTEAASVVLADRAFRGPPSDSYPINIQQLALLS 372
           DA+HPTE  ++++A +AF G  + +YPINIQQLA L+
Sbjct: 353 DAFHPTEKVNLIMAKKAFAGDRTVAYPINIQQLASLN 384

BLAST of CmaCh18G002870 vs. TAIR10
Match: AT5G15720.1 (AT5G15720.1 GDSL-motif lipase 7)

HSP 1 Score: 338.6 bits (867), Expect = 4.9e-93
Identity = 172/338 (50.89%), Postives = 227/338 (67.16%), Query Frame = 1

Query: 37  PAIFVFGDSLVDVGNNNFLHSAARANFYPYGIDFRGGPTGRFSNGRTVIDMIVDYLGIPN 96
           PA FVFGDSLVD GNNN++ + ARAN++PYGIDF G PTGRF NGRTV+D    YLG+P 
Sbjct: 29  PAFFVFGDSLVDSGNNNYIPTLARANYFPYGIDF-GFPTGRFCNGRTVVDYGATYLGLPL 88

Query: 97  AQEFANPDTSGDRILKGVNYASAAAGILDETGRHYGERYSLNQQVVNFESTRN-DLRKSM 156
              + +P + G   L+GVNYASAAAGILDETGRHYG R + N Q+  FE T    LR+  
Sbjct: 89  VPPYLSPLSIGQNALRGVNYASAAAGILDETGRHYGARTTFNGQISQFEITIELRLRRFF 148

Query: 157 DN-WNLTRYLSRSITFMAFGSNDYINNYLMPNLYTSRFRYTSDQFADHLLNSYTRQLLAL 216
            N  +L +YL++SI  +  GSNDYINNYLMP  Y++   Y+ + +AD L+ + + Q+  L
Sbjct: 149 QNPADLRKYLAKSIIGINIGSNDYINNYLMPERYSTSQTYSGEDYADLLIKTLSAQISRL 208

Query: 217 RSAGLRKFVVAGIGPLGCIPNQRA--TGVALPGRCVDNVNEMLGDFNEGLRSLVTQLNSQ 276
            + G RK V+AG GPLGCIP+Q +  TG    G CV  +N M+  FN  L+ L   LN+ 
Sbjct: 209 YNLGARKMVLAGSGPLGCIPSQLSMVTGNNTSG-CVTKINNMVSMFNSRLKDLANTLNTT 268

Query: 277 YPDSYFVYTNIYGIFGDILNHPDTYGFRVVDTACCGVGLNRGQITCLPLQFPCLNRNEYA 336
            P S+FVY N++ +F D++ +P  YG  V + ACCG G   G +TCLPLQ PCL+RN+Y 
Sbjct: 269 LPGSFFVYQNVFDLFHDMVVNPSRYGLVVSNEACCGNGRYGGALTCLPLQQPCLDRNQYV 328

Query: 337 FWDAYHPTEAASVVLADRAFRGPPSDSYPINIQQLALL 371
           FWDA+HPTE A+ ++A   F    + SYPI++ +LA L
Sbjct: 329 FWDAFHPTETANKIIAHNTFSKSANYSYPISVYELAKL 364

BLAST of CmaCh18G002870 vs. NCBI nr
Match: gi|659067604|ref|XP_008440329.1| (PREDICTED: GDSL esterase/lipase At1g71250 [Cucumis melo])

HSP 1 Score: 637.1 bits (1642), Expect = 1.9e-179
Identity = 307/360 (85.28%), Postives = 330/360 (91.67%), Query Frame = 1

Query: 12  ATATLVLVLVLFECSKQMALAEN-GVPAIFVFGDSLVDVGNNNFLHSAARANFYPYGIDF 71
           A A  V++LV  +CS QM  AEN GVPAIFVFGDSLVDVGNNNFLHSAARAN+YPYGIDF
Sbjct: 11  AAAAGVVLLVFLKCSNQMVAAENYGVPAIFVFGDSLVDVGNNNFLHSAARANYYPYGIDF 70

Query: 72  RGGPTGRFSNGRTVIDMIVDYLGIPNAQEFANPDTSGDRILKGVNYASAAAGILDETGRH 131
             GPTGRFSNGRTVIDM VD LGIPNA EFANPDTSGDRIL GVNYASAAAGILDETGRH
Sbjct: 71  SDGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRILNGVNYASAAAGILDETGRH 130

Query: 132 YGERYSLNQQVVNFESTRNDLRKSMDNWNLTRYLSRSITFMAFGSNDYINNYLMPNLYTS 191
           YG+RYSL+QQVVNFEST NDLR+SM +WNLTRYLS+SI F+AFGSNDYINNYLMPNLYT+
Sbjct: 131 YGDRYSLSQQVVNFESTLNDLRRSMGSWNLTRYLSKSIAFLAFGSNDYINNYLMPNLYTT 190

Query: 192 RFRYTSDQFADHLLNSYTRQLLALRSAGLRKFVVAGIGPLGCIPNQRATGVALPGRCVDN 251
           RFRY S+QFA+ LLN Y+RQLLALRS GLRK VVAG+GPLGCIPNQRATG+ +PGRC DN
Sbjct: 191 RFRYNSNQFANLLLNRYSRQLLALRSVGLRKLVVAGVGPLGCIPNQRATGLTIPGRCADN 250

Query: 252 VNEMLGDFNEGLRSLVTQLNSQYPDSYFVYTNIYGIFGDILNHPDTYGFRVVDTACCGVG 311
           VNEMLG FNEGL+SLVTQLNSQYPDS FVYTNIYGIFGDILN+P+TYGFRVVDTACCGVG
Sbjct: 251 VNEMLGAFNEGLKSLVTQLNSQYPDSNFVYTNIYGIFGDILNNPETYGFRVVDTACCGVG 310

Query: 312 LNRGQITCLPLQFPCLNRNEYAFWDAYHPTEAASVVLADRAFRGPPSDSYPINIQQLALL 371
           LNRGQITCLPLQFPCLNRNEY FWDA+HPTEAAS VLA RAFRGPPSDSYPIN+QQLAL+
Sbjct: 311 LNRGQITCLPLQFPCLNRNEYVFWDAFHPTEAASNVLAGRAFRGPPSDSYPINVQQLALI 370

BLAST of CmaCh18G002870 vs. NCBI nr
Match: gi|700209361|gb|KGN64457.1| (hypothetical protein Csa_1G056930 [Cucumis sativus])

HSP 1 Score: 623.6 bits (1607), Expect = 2.2e-175
Identity = 297/359 (82.73%), Postives = 326/359 (90.81%), Query Frame = 1

Query: 12  ATATLVLVLVLFECSKQMALAENGVPAIFVFGDSLVDVGNNNFLHSAARANFYPYGIDFR 71
           A A  V+VLV  + S QM  AENGV AIFVFGDSLVDVGNNNFLHSAARAN+YPYG+DF 
Sbjct: 2   AAAAGVVVLVFLKSSYQMVEAENGVSAIFVFGDSLVDVGNNNFLHSAARANYYPYGVDFT 61

Query: 72  GGPTGRFSNGRTVIDMIVDYLGIPNAQEFANPDTSGDRILKGVNYASAAAGILDETGRHY 131
            GPTGRFSNGRTVIDM VD LGIPNA EF+NPDTSGDRIL GVNYASAAAGILDETGRHY
Sbjct: 62  DGPTGRFSNGRTVIDMFVDMLGIPNAPEFSNPDTSGDRILNGVNYASAAAGILDETGRHY 121

Query: 132 GERYSLNQQVVNFESTRNDLRKSMDNWNLTRYLSRSITFMAFGSNDYINNYLMPNLYTSR 191
           G+RY+L+QQVVNFEST NDLR+SM +WNLTRYLS+SI F+AFGSNDYINNYLMPNLYT+R
Sbjct: 122 GDRYTLSQQVVNFESTLNDLRRSMGSWNLTRYLSKSIAFLAFGSNDYINNYLMPNLYTTR 181

Query: 192 FRYTSDQFADHLLNSYTRQLLALRSAGLRKFVVAGIGPLGCIPNQRATGVALPGRCVDNV 251
           FRY S+QFA+ LLN Y+RQLLAL+S G++K V+AG+GPLGCIPNQRATGV LPGRC D V
Sbjct: 182 FRYNSNQFANLLLNRYSRQLLALQSVGVKKLVIAGLGPLGCIPNQRATGVTLPGRCADKV 241

Query: 252 NEMLGDFNEGLRSLVTQLNSQYPDSYFVYTNIYGIFGDILNHPDTYGFRVVDTACCGVGL 311
           NEMLG FNEGL+SLVTQLNSQYPD+ FVYTNIYGIFGDILN+P+TYGF VVDTACCGVGL
Sbjct: 242 NEMLGAFNEGLKSLVTQLNSQYPDTKFVYTNIYGIFGDILNNPETYGFSVVDTACCGVGL 301

Query: 312 NRGQITCLPLQFPCLNRNEYAFWDAYHPTEAASVVLADRAFRGPPSDSYPINIQQLALL 371
           NRGQITCLPLQFPCLNRNEY FWDA+HPTEAAS +LA RAFRGPPSDSYPIN+QQLAL+
Sbjct: 302 NRGQITCLPLQFPCLNRNEYVFWDAFHPTEAASYILAGRAFRGPPSDSYPINVQQLALI 360

BLAST of CmaCh18G002870 vs. NCBI nr
Match: gi|470107890|ref|XP_004290271.1| (PREDICTED: GDSL esterase/lipase At1g71250 [Fragaria vesca subsp. vesca])

HSP 1 Score: 499.6 bits (1285), Expect = 4.8e-138
Identity = 238/363 (65.56%), Postives = 290/363 (79.89%), Query Frame = 1

Query: 13  TATLVLVLVLFECSKQMAL-----AENGVPAIFVFGDSLVDVGNNNFLHSAARANFYPYG 72
           T ++V++ +L  CS    +     A   VPA+F FGDSL+DVGNNNF+ S AR+N+YPYG
Sbjct: 24  TISVVIMKILISCSMVAGVGFGESAAAQVPAMFAFGDSLIDVGNNNFISSFARSNYYPYG 83

Query: 73  IDFRGGPTGRFSNGRTVIDMIVDYLGIPNAQEFANPDTSGDRILKGVNYASAAAGILDET 132
           IDFRGGP+GRF+NGRT IDM+ + LG+P A  FA+P+T G+RIL+GVNYASAAAGILDET
Sbjct: 84  IDFRGGPSGRFTNGRTSIDMLGNLLGLPYAPAFADPNTKGNRILRGVNYASAAAGILDET 143

Query: 133 GRHYGERYSLNQQVVNFESTRNDLRKSMDNWNLTRYLSRSITFMAFGSNDYINNYLMPNL 192
           GRHYG+RYSL++QV+NFEST N LR  M   NLT+YL++SI  + FGSNDYINNYLMP  
Sbjct: 144 GRHYGDRYSLSRQVMNFESTLNQLRTMMGGTNLTQYLAKSIAVLVFGSNDYINNYLMP-F 203

Query: 193 YTSRFRYTSDQFADHLLNSYTRQLLALRSAGLRKFVVAGIGPLGCIPNQRATGVALPGRC 252
           Y S + Y+   FA+ LL+ Y  Q+LAL S GLRKF++ GIGPLGCIPNQRA+G A PGRC
Sbjct: 204 YNSSYTYSPPAFANLLLSRYATQILALHSLGLRKFMLVGIGPLGCIPNQRASGRAQPGRC 263

Query: 253 VDNVNEMLGDFNEGLRSLVTQLNSQYPDSYFVYTNIYGIFGDILNHPDTYGFRVVDTACC 312
           VDNVN++LG FN+GLRSL TQLNS +P + FVY N YG FGDILN+P +YGF V+D ACC
Sbjct: 264 VDNVNQILGSFNQGLRSLATQLNSNHPGAIFVYGNAYGAFGDILNNPLSYGFTVIDRACC 323

Query: 313 GVGLNRGQITCLPLQFPCLNRNEYAFWDAYHPTEAASVVLADRAFRGPPSDSYPINIQQL 371
           G+G N+GQITCLP   PC NRN+Y FWDA+HPTEAA+ VLA RAF GPPSDSYPIN+QQ+
Sbjct: 324 GIGRNQGQITCLPFAVPCSNRNQYMFWDAFHPTEAANAVLAWRAFNGPPSDSYPINVQQM 383

BLAST of CmaCh18G002870 vs. NCBI nr
Match: gi|645264717|ref|XP_008237811.1| (PREDICTED: GDSL esterase/lipase At1g71250 [Prunus mume])

HSP 1 Score: 491.9 bits (1265), Expect = 9.9e-136
Identity = 230/358 (64.25%), Postives = 287/358 (80.17%), Query Frame = 1

Query: 16  LVLVLVLFECSKQM---ALAENGVPAIFVFGDSLVDVGNNNFLHSAARANFYPYGIDFRG 75
           L++++++ +CS  +   + A   VPA+F+FGDSL+DVGNNN+L S A++N+YPYG DFR 
Sbjct: 27  LIMMILVVQCSTSIGSVSAAAAQVPAMFIFGDSLIDVGNNNYLSSFAKSNYYPYGCDFRA 86

Query: 76  GPTGRFSNGRTVIDMIVDYLGIPNAQEFANPDTSGDRILKGVNYASAAAGILDETGRHYG 135
           GPTGRFSNGRTV+DM+ + LG+P    FA+P+T G +IL GVNYASAAAGILDETG+H+G
Sbjct: 87  GPTGRFSNGRTVVDMLGNLLGLPYVPPFADPNTKGTKILGGVNYASAAAGILDETGQHFG 146

Query: 136 ERYSLNQQVVNFESTRNDLRKSMDNWNLTRYLSRSITFMAFGSNDYINNYLMPNLYTSRF 195
           +RYSL+QQV+NFEST + LR  M   N+T+YL++SI  + FGSNDYINNYL+P+LY+S +
Sbjct: 147 QRYSLSQQVLNFESTLDQLRPMMSGANMTKYLAKSIAVLEFGSNDYINNYLLPSLYSSSY 206

Query: 196 RYTSDQFADHLLNSYTRQLLALRSAGLRKFVVAGIGPLGCIPNQRATGVALPGRCVDNVN 255
            YT   FA+ LLN YT+Q+LAL S GLRKFV+AGIGPLGCIPNQRA  +A PGRCVD VN
Sbjct: 207 IYTPPAFANLLLNRYTQQILALHSVGLRKFVLAGIGPLGCIPNQRA--LAQPGRCVDYVN 266

Query: 256 EMLGDFNEGLRSLVTQLNSQYPDSYFVYTNIYGIFGDILNHPDTYGFRVVDTACCGVGLN 315
           ++LG FNEGLRSL  QLN+ +P + FVY N Y  FGD+LN+P  YGF V+D  CCG+G N
Sbjct: 267 QILGSFNEGLRSLANQLNTNHPGAIFVYANTYAAFGDMLNNPALYGFSVIDRGCCGIGRN 326

Query: 316 RGQITCLPLQFPCLNRNEYAFWDAYHPTEAASVVLADRAFRGPPSDSYPINIQQLALL 371
           RGQITCLP   PC NRN+Y FWDAYHPTEAA+ VLA RAF GPPSD YPIN+QQ+AL+
Sbjct: 327 RGQITCLPYSVPCANRNQYIFWDAYHPTEAANAVLAWRAFNGPPSDCYPINVQQIALI 382

BLAST of CmaCh18G002870 vs. NCBI nr
Match: gi|657979451|ref|XP_008381690.1| (PREDICTED: GDSL esterase/lipase At1g71250 [Malus domestica])

HSP 1 Score: 490.7 bits (1262), Expect = 2.2e-135
Identity = 227/335 (67.76%), Postives = 275/335 (82.09%), Query Frame = 1

Query: 36  VPAIFVFGDSLVDVGNNNFLHSAARANFYPYGIDFRGGPTGRFSNGRTVIDMIVDYLGIP 95
           VPA+FVFGDSL+DVGNNNFL+S A++N+YPYG DFR GPTGRF+NGRT +D++ + LG+P
Sbjct: 38  VPAMFVFGDSLIDVGNNNFLNSLAKSNYYPYGCDFRAGPTGRFTNGRTFVDLLGNLLGLP 97

Query: 96  NAQEFANPDTSGDRILKGVNYASAAAGILDETGRHYGERYSLNQQVVNFESTRNDLRKSM 155
               FA+P+T G R+L GVNYASAAAGILDETG+HYGERY L+QQV+NFEST N LR  M
Sbjct: 98  YVPAFADPNTKGTRMLGGVNYASAAAGILDETGQHYGERYXLSQQVLNFESTLNQLRSMM 157

Query: 156 DNWNLTRYLSRSITFMAFGSNDYINNYLMPNLYTSRFRYTSDQFADHLLNSYTRQLLALR 215
              NLT+ L++SI  +AFGSNDYINNYL+P+LYTS + YT   FA+ LLN YTRQ+LAL 
Sbjct: 158 SGTNLTQMLAKSIAVLAFGSNDYINNYLLPSLYTSSYYYTPPAFANLLLNRYTRQILALH 217

Query: 216 SAGLRKFVVAGIGPLGCIPNQRATGVALPGRCVDNVNEMLGDFNEGLRSLVTQLNSQYPD 275
           S GLRKFV+AGIGPLGCIPNQRA+  A P RC+D VN++LG +NEGLRSLV Q N+ +PD
Sbjct: 218 SVGLRKFVLAGIGPLGCIPNQRAS-AAQPERCLDYVNQILGTYNEGLRSLVNQFNTNHPD 277

Query: 276 SYFVYTNIYGIFGDILNHPDTYGFRVVDTACCGVGLNRGQITCLPLQFPCLNRNEYAFWD 335
           + FVY N YG FGD+LN+P  YGF V+D  CCG+G NRGQITCLP   PC NRN+Y FWD
Sbjct: 278 AIFVYANTYGAFGDMLNNPAPYGFSVIDRGCCGIGRNRGQITCLPYAVPCSNRNQYMFWD 337

Query: 336 AYHPTEAASVVLADRAFRGPPSDSYPINIQQLALL 371
           A+HPTEAA+ +LA RA+ GPPSDSYPIN+QQ+AL+
Sbjct: 338 AFHPTEAANAILAWRAYNGPPSDSYPINVQQMALI 371

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
GDL28_ARATH6.0e-12560.28GDSL esterase/lipase At1g71250 OS=Arabidopsis thaliana GN=At1g71250 PE=2 SV=1[more]
GDL75_ARATH6.5e-10353.42GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana GN=At5g08460 PE=2 SV=1[more]
GDL15_ARATH2.2e-9551.54GDSL esterase/lipase At1g29670 OS=Arabidopsis thaliana GN=At1g29670 PE=2 SV=1[more]
GDL29_ARATH6.1e-9349.55GDSL esterase/lipase At1g71691 OS=Arabidopsis thaliana GN=At1g71691 PE=2 SV=1[more]
GLIP7_ARATH8.8e-9250.89GDSL esterase/lipase 7 OS=Arabidopsis thaliana GN=GLIP7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LUJ9_CUCSA1.5e-17582.73Uncharacterized protein OS=Cucumis sativus GN=Csa_1G056930 PE=4 SV=1[more]
F6GVY3_VITVI9.3e-13362.33Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0089g00940 PE=4 SV=... [more]
M5VKG0_PRUPE4.6e-13266.57Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008473mg PE=4 SV=1[more]
K4D878_SOLLC1.1e-12864.41Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1[more]
A0A061FQW3_THECC1.4e-12862.64GDSL-like Lipase/Acylhydrolase superfamily protein isoform 1 OS=Theobroma cacao ... [more]
Match NameE-valueIdentityDescription
AT1G71250.13.4e-12660.28 GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT5G08460.13.7e-10453.42 GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT1G29670.11.3e-9651.54 GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT1G71691.23.4e-9449.55 GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT5G15720.14.9e-9350.89 GDSL-motif lipase 7[more]
Match NameE-valueIdentityDescription
gi|659067604|ref|XP_008440329.1|1.9e-17985.28PREDICTED: GDSL esterase/lipase At1g71250 [Cucumis melo][more]
gi|700209361|gb|KGN64457.1|2.2e-17582.73hypothetical protein Csa_1G056930 [Cucumis sativus][more]
gi|470107890|ref|XP_004290271.1|4.8e-13865.56PREDICTED: GDSL esterase/lipase At1g71250 [Fragaria vesca subsp. vesca][more]
gi|645264717|ref|XP_008237811.1|9.9e-13664.25PREDICTED: GDSL esterase/lipase At1g71250 [Prunus mume][more]
gi|657979451|ref|XP_008381690.1|2.2e-13567.76PREDICTED: GDSL esterase/lipase At1g71250 [Malus domestica][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001087GDSL
Vocabulary: Molecular Function
TermDefinition
GO:0016788hydrolase activity, acting on ester bonds
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016788 hydrolase activity, acting on ester bonds

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh18G002870.1CmaCh18G002870.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 39..349
score: 4.9
NoneNo IPR availablePANTHERPTHR22835ZINC FINGER FYVE DOMAIN CONTAINING PROTEINcoord: 5..367
score: 1.0E
NoneNo IPR availablePANTHERPTHR22835:SF168SUBFAMILY NOT NAMEDcoord: 5..367
score: 1.0E