CmaCh16G012150 (gene) Cucurbita maxima (Rimu)

NameCmaCh16G012150
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionReplication protein A 70 kDa DNA-binding subunit
LocationCma_Chr16 : 9203535 .. 9203978 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAAGTTCAGGGCAGGGAACACCATACAGAATCCTGGAGTTTTTCGCCCCAGATCGGAGAATATAGGTGGTGGACAGTTCGTGTCACCCATTCGAAATAGTACCGATGTTAGCAGAGAATATGGTACACCGAACCGAGGTGTTCAGTATGGGGACCATCACAACAGTTGTAGGCCTACTCCATCCACACTGCATAACTCGCGCACGTACTGCAACAGCTGTGGGGGATTGGGTCACAGCTCAATGAACTGTCCGAGTATCATCAGCAGCCCTGCGCCATCCGTTGGAGGAGGGATGTACTTAAATCGAGTTTCAGGCTCGTCTGGTCGTGACTCTGGTGAATGCTTCAAATGCCATCAAACCGGGCACTGGGCTAGGGATTGCCCTGGTTTGGCCACTTTCCCTCCAGCTTATGGTAACAGTGGCTTCACAGCTAGGTGA

mRNA sequence

ATGGAAAAGTTCAGGGCAGGGAACACCATACAGAATCCTGGAGTTTTTCGCCCCAGATCGGAGAATATAGGTGGTGGACAGTTCGTGTCACCCATTCGAAATAGTACCGATGTTAGCAGAGAATATGGTACACCGAACCGAGGTGTTCAGTATGGGGACCATCACAACAGTTGTAGGCCTACTCCATCCACACTGCATAACTCGCGCACGTACTGCAACAGCTGTGGGGGATTGGGTCACAGCTCAATGAACTGTCCGAGTATCATCAGCAGCCCTGCGCCATCCGTTGGAGGAGGGATGTACTTAAATCGAGTTTCAGGCTCGTCTGGTCGTGACTCTGGTGAATGCTTCAAATGCCATCAAACCGGGCACTGGGCTAGGGATTGCCCTGGTTTGGCCACTTTCCCTCCAGCTTATGGTAACAGTGGCTTCACAGCTAGGTGA

Coding sequence (CDS)

ATGGAAAAGTTCAGGGCAGGGAACACCATACAGAATCCTGGAGTTTTTCGCCCCAGATCGGAGAATATAGGTGGTGGACAGTTCGTGTCACCCATTCGAAATAGTACCGATGTTAGCAGAGAATATGGTACACCGAACCGAGGTGTTCAGTATGGGGACCATCACAACAGTTGTAGGCCTACTCCATCCACACTGCATAACTCGCGCACGTACTGCAACAGCTGTGGGGGATTGGGTCACAGCTCAATGAACTGTCCGAGTATCATCAGCAGCCCTGCGCCATCCGTTGGAGGAGGGATGTACTTAAATCGAGTTTCAGGCTCGTCTGGTCGTGACTCTGGTGAATGCTTCAAATGCCATCAAACCGGGCACTGGGCTAGGGATTGCCCTGGTTTGGCCACTTTCCCTCCAGCTTATGGTAACAGTGGCTTCACAGCTAGGTGA

Protein sequence

MEKFRAGNTIQNPGVFRPRSENIGGGQFVSPIRNSTDVSREYGTPNRGVQYGDHHNSCRPTPSTLHNSRTYCNSCGGLGHSSMNCPSIISSPAPSVGGGMYLNRVSGSSGRDSGECFKCHQTGHWARDCPGLATFPPAYGNSGFTAR
BLAST of CmaCh16G012150 vs. Swiss-Prot
Match: RFA1C_ORYSJ (Replication protein A 70 kDa DNA-binding subunit C OS=Oryza sativa subsp. japonica GN=RPA1C PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 3.8e-06
Identity = 27/75 (36.00%), Postives = 37/75 (49.33%), Query Frame = 1

Query: 61  TPSTLHNSRTYCNSCGGLGHSSMNCP-SIISSPAPSVGGGMYLNRVSGSSGRDSGECFKC 120
           TP     +   C+ CG  GHS+  C          +  GG  +   +  +G  S EC+KC
Sbjct: 805 TPLAATRNLQTCSICGANGHSAQICHVGADMDMQETSAGGSSMGNYNSIAGNGSSECYKC 864

Query: 121 HQTGHWARDCPGLAT 135
            Q GH+ARDCPG +T
Sbjct: 865 KQPGHYARDCPGQST 879

BLAST of CmaCh16G012150 vs. TrEMBL
Match: A0A0A0L503_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G005560 PE=4 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 1.4e-55
Identity = 107/143 (74.83%), Postives = 117/143 (81.82%), Query Frame = 1

Query: 5   RAGNTIQNPGVFRPRSENIGGGQFVSPIRNSTDVSREYGTPNRGVQYGDHHNSCRPTPST 64
           +A +TI N G     SENIGGGQFVSPIRNST+ SREYGTPN+GVQYG+ ++S RP+PST
Sbjct: 719 KAESTIHNFGFRDTASENIGGGQFVSPIRNSTNFSREYGTPNQGVQYGNQYSSSRPSPST 778

Query: 65  LHNSRTYCNSCGGLGHSSMNCPSIISSPAPSVGGGMYLNRVSGSSGRDSGECFKCHQTGH 124
             NS TYCNSCGG GHSS NCPSI+S PA SVGGGMY N+ SG SG  SGECFKCHQTGH
Sbjct: 779 PLNSNTYCNSCGGSGHSSTNCPSIMSGPALSVGGGMYSNQASGPSGGTSGECFKCHQTGH 838

Query: 125 WARDCPGLATFPPAYGNSGFTAR 148
           WARDCPGLA  PPAYGN GFTAR
Sbjct: 839 WARDCPGLANVPPAYGNRGFTAR 861

BLAST of CmaCh16G012150 vs. TrEMBL
Match: A5AQN6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_038295 PE=4 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.0e-26
Identity = 68/145 (46.90%), Postives = 92/145 (63.45%), Query Frame = 1

Query: 5   RAGNTIQNPGVFRPRSENIGGGQFVSPIR---NSTDVSREYGTP-NRGVQYGDHHNSCRP 64
           ++ N I N G+  P   N+G  Q    +    N+++  R++G P N+GVQ+G+ + S   
Sbjct: 722 KSENVISNGGMTNPGLGNVGIRQSTPSVNYSGNASNAGRDFGFPANQGVQHGNQYGSSGL 781

Query: 65  TPSTLHNSRTYCNSCGGLGHSSMNCPSIISSPAPSVGGGMYLNRVSG--SSGRDSGECFK 124
           + +        CNSCGG GHSS NCPS++ SP  S GGG Y++R S   S+G  +GEC+K
Sbjct: 782 SATGSTGMXQSCNSCGGTGHSSSNCPSVMHSPRQSSGGG-YVSRASTGPSAGGTTGECYK 841

Query: 125 CHQTGHWARDCPGLATFPPAYGNSG 144
           CHQ GHWARDCPGL T PPAYG+SG
Sbjct: 842 CHQFGHWARDCPGLNTGPPAYGSSG 865

BLAST of CmaCh16G012150 vs. TrEMBL
Match: F6I092_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0033g01170 PE=4 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.4e-26
Identity = 68/145 (46.90%), Postives = 92/145 (63.45%), Query Frame = 1

Query: 5   RAGNTIQNPGVFRPRSENIGGGQFVSPIR---NSTDVSREYGTP-NRGVQYGDHHNSCRP 64
           ++ N I N G+  P   N+G  Q    +    N+++  R++G P N+GVQ+G+ + S   
Sbjct: 722 KSENVISNGGMTNPGLGNVGIRQSTPSVNYSGNASNAGRDFGFPANQGVQHGNQYGSSGL 781

Query: 65  TPSTLHNSRTYCNSCGGLGHSSMNCPSIISSPAPSVGGGMYLNRVSG--SSGRDSGECFK 124
           + +        CNSCGG GHSS NCPS++ SP  S GGG Y++R S   S+G  +GEC+K
Sbjct: 782 SATGSTGMYQSCNSCGGTGHSSSNCPSVMHSPRQSSGGG-YVSRASTGPSAGGTTGECYK 841

Query: 125 CHQTGHWARDCPGLATFPPAYGNSG 144
           CHQ GHWARDCPGL T PPAYG+SG
Sbjct: 842 CHQFGHWARDCPGLNTGPPAYGSSG 865

BLAST of CmaCh16G012150 vs. TrEMBL
Match: A0A067E5M0_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g003281mg PE=4 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 4.5e-22
Identity = 61/144 (42.36%), Postives = 80/144 (55.56%), Query Frame = 1

Query: 5   RAGNTIQNPGVFRPRSENIGGGQFVSPIRNSTDVSREYGTP-----NRGVQYGDHHNSCR 64
           +A N I  P +    + NIG  Q    + N    +   G       N+  QYG+ ++  R
Sbjct: 672 KAENIIPKPEMTYTGTGNIGSRQTAPVVANYVGNNSNAGAASGLLANQMGQYGNQYSGSR 731

Query: 65  PTPSTLHNSRTYCNSCGGLGHSSMNCPSIISSPAPSVGGGMYLNRVSGSSGRDSGECFKC 124
              +    +R  C+SCG +GHSSMNCPSI+S P  S GGG   +  SG+    S EC+KC
Sbjct: 732 LPAAGTSAARQSCSSCGAVGHSSMNCPSIMSGPGQSSGGGYANSFSSGAGIATSSECYKC 791

Query: 125 HQTGHWARDCPGLATFPPAYGNSG 144
           H+TGHWARDCP L   PPAYG+SG
Sbjct: 792 HKTGHWARDCPSLNAAPPAYGSSG 815

BLAST of CmaCh16G012150 vs. TrEMBL
Match: A0A151THH2_CAJCA (Replication protein A 70 kDa DNA-binding subunit OS=Cajanus cajan GN=KK1_012785 PE=4 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 4.5e-22
Identity = 60/118 (50.85%), Postives = 78/118 (66.10%), Query Frame = 1

Query: 30  SPIRNSTDVSREYGTP-NRGVQYGDHHN--SCRPTPSTLHNSRTYCNSCGGLGHSSMNCP 89
           +PI++STD SR+YG P N+  QYG+ +N  S    PS+  +S   C +CGG GHSS+ CP
Sbjct: 639 NPIKSSTDTSRDYGMPANQVGQYGNQYNNFSSAGAPSSYSSS---CTNCGGSGHSSVLCP 698

Query: 90  SIISSPAPSVGGGMYLNRVSGSSGRD--SGECFKCHQTGHWARDCPGLATFPPAYGNS 143
           ++ + P    GGG + NR S  SG    +GECFKCHQ GHWARDCPG +  PPAYG +
Sbjct: 699 NVRNVPGQYAGGG-FSNRSSAGSGGGGGAGECFKCHQPGHWARDCPGFSAAPPAYGGT 752

BLAST of CmaCh16G012150 vs. NCBI nr
Match: gi|659095462|ref|XP_008448593.1| (PREDICTED: replication protein A 70 kDa DNA-binding subunit A [Cucumis melo])

HSP 1 Score: 224.2 bits (570), Expect = 1.5e-55
Identity = 107/143 (74.83%), Postives = 117/143 (81.82%), Query Frame = 1

Query: 5   RAGNTIQNPGVFRPRSENIGGGQFVSPIRNSTDVSREYGTPNRGVQYGDHHNSCRPTPST 64
           +A +TI N G     SENIGGGQFVSPIRNST+ SREYGTPN+GVQYG+ ++S RP+PST
Sbjct: 719 KAESTIHNLGFRDTASENIGGGQFVSPIRNSTNFSREYGTPNQGVQYGNQYSSSRPSPST 778

Query: 65  LHNSRTYCNSCGGLGHSSMNCPSIISSPAPSVGGGMYLNRVSGSSGRDSGECFKCHQTGH 124
             NS TYCNSCGG GHSSMNCPSI+S PA SVGGGMY N+ SG S   SGECFKCHQTGH
Sbjct: 779 PLNSNTYCNSCGGSGHSSMNCPSIMSGPALSVGGGMYSNQASGPSAGTSGECFKCHQTGH 838

Query: 125 WARDCPGLATFPPAYGNSGFTAR 148
           WARDCPGLA  PPAYGN GFTAR
Sbjct: 839 WARDCPGLANVPPAYGNRGFTAR 861

BLAST of CmaCh16G012150 vs. NCBI nr
Match: gi|449456771|ref|XP_004146122.1| (PREDICTED: replication protein A 70 kDa DNA-binding subunit A [Cucumis sativus])

HSP 1 Score: 223.8 bits (569), Expect = 2.0e-55
Identity = 107/143 (74.83%), Postives = 117/143 (81.82%), Query Frame = 1

Query: 5   RAGNTIQNPGVFRPRSENIGGGQFVSPIRNSTDVSREYGTPNRGVQYGDHHNSCRPTPST 64
           +A +TI N G     SENIGGGQFVSPIRNST+ SREYGTPN+GVQYG+ ++S RP+PST
Sbjct: 719 KAESTIHNFGFRDTASENIGGGQFVSPIRNSTNFSREYGTPNQGVQYGNQYSSSRPSPST 778

Query: 65  LHNSRTYCNSCGGLGHSSMNCPSIISSPAPSVGGGMYLNRVSGSSGRDSGECFKCHQTGH 124
             NS TYCNSCGG GHSS NCPSI+S PA SVGGGMY N+ SG SG  SGECFKCHQTGH
Sbjct: 779 PLNSNTYCNSCGGSGHSSTNCPSIMSGPALSVGGGMYSNQASGPSGGTSGECFKCHQTGH 838

Query: 125 WARDCPGLATFPPAYGNSGFTAR 148
           WARDCPGLA  PPAYGN GFTAR
Sbjct: 839 WARDCPGLANVPPAYGNRGFTAR 861

BLAST of CmaCh16G012150 vs. NCBI nr
Match: gi|147856620|emb|CAN82475.1| (hypothetical protein VITISV_038295 [Vitis vinifera])

HSP 1 Score: 127.9 bits (320), Expect = 1.5e-26
Identity = 68/145 (46.90%), Postives = 92/145 (63.45%), Query Frame = 1

Query: 5   RAGNTIQNPGVFRPRSENIGGGQFVSPIR---NSTDVSREYGTP-NRGVQYGDHHNSCRP 64
           ++ N I N G+  P   N+G  Q    +    N+++  R++G P N+GVQ+G+ + S   
Sbjct: 722 KSENVISNGGMTNPGLGNVGIRQSTPSVNYSGNASNAGRDFGFPANQGVQHGNQYGSSGL 781

Query: 65  TPSTLHNSRTYCNSCGGLGHSSMNCPSIISSPAPSVGGGMYLNRVSG--SSGRDSGECFK 124
           + +        CNSCGG GHSS NCPS++ SP  S GGG Y++R S   S+G  +GEC+K
Sbjct: 782 SATGSTGMXQSCNSCGGTGHSSSNCPSVMHSPRQSSGGG-YVSRASTGPSAGGTTGECYK 841

Query: 125 CHQTGHWARDCPGLATFPPAYGNSG 144
           CHQ GHWARDCPGL T PPAYG+SG
Sbjct: 842 CHQFGHWARDCPGLNTGPPAYGSSG 865

BLAST of CmaCh16G012150 vs. NCBI nr
Match: gi|225427514|ref|XP_002264009.1| (PREDICTED: replication protein A 70 kDa DNA-binding subunit A [Vitis vinifera])

HSP 1 Score: 127.5 bits (319), Expect = 1.9e-26
Identity = 68/145 (46.90%), Postives = 92/145 (63.45%), Query Frame = 1

Query: 5   RAGNTIQNPGVFRPRSENIGGGQFVSPIR---NSTDVSREYGTP-NRGVQYGDHHNSCRP 64
           ++ N I N G+  P   N+G  Q    +    N+++  R++G P N+GVQ+G+ + S   
Sbjct: 722 KSENVISNGGMTNPGLGNVGIRQSTPSVNYSGNASNAGRDFGFPANQGVQHGNQYGSSGL 781

Query: 65  TPSTLHNSRTYCNSCGGLGHSSMNCPSIISSPAPSVGGGMYLNRVSG--SSGRDSGECFK 124
           + +        CNSCGG GHSS NCPS++ SP  S GGG Y++R S   S+G  +GEC+K
Sbjct: 782 SATGSTGMYQSCNSCGGTGHSSSNCPSVMHSPRQSSGGG-YVSRASTGPSAGGTTGECYK 841

Query: 125 CHQTGHWARDCPGLATFPPAYGNSG 144
           CHQ GHWARDCPGL T PPAYG+SG
Sbjct: 842 CHQFGHWARDCPGLNTGPPAYGSSG 865

BLAST of CmaCh16G012150 vs. NCBI nr
Match: gi|296088465|emb|CBI37456.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 127.5 bits (319), Expect = 1.9e-26
Identity = 68/145 (46.90%), Postives = 92/145 (63.45%), Query Frame = 1

Query: 5   RAGNTIQNPGVFRPRSENIGGGQFVSPIR---NSTDVSREYGTP-NRGVQYGDHHNSCRP 64
           ++ N I N G+  P   N+G  Q    +    N+++  R++G P N+GVQ+G+ + S   
Sbjct: 543 KSENVISNGGMTNPGLGNVGIRQSTPSVNYSGNASNAGRDFGFPANQGVQHGNQYGSSGL 602

Query: 65  TPSTLHNSRTYCNSCGGLGHSSMNCPSIISSPAPSVGGGMYLNRVSG--SSGRDSGECFK 124
           + +        CNSCGG GHSS NCPS++ SP  S GGG Y++R S   S+G  +GEC+K
Sbjct: 603 SATGSTGMYQSCNSCGGTGHSSSNCPSVMHSPRQSSGGG-YVSRASTGPSAGGTTGECYK 662

Query: 125 CHQTGHWARDCPGLATFPPAYGNSG 144
           CHQ GHWARDCPGL T PPAYG+SG
Sbjct: 663 CHQFGHWARDCPGLNTGPPAYGSSG 686

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RFA1C_ORYSJ3.8e-0636.00Replication protein A 70 kDa DNA-binding subunit C OS=Oryza sativa subsp. japoni... [more]
Match NameE-valueIdentityDescription
A0A0A0L503_CUCSA1.4e-5574.83Uncharacterized protein OS=Cucumis sativus GN=Csa_3G005560 PE=4 SV=1[more]
A5AQN6_VITVI1.0e-2646.90Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_038295 PE=4 SV=1[more]
F6I092_VITVI1.4e-2646.90Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0033g01170 PE=4 SV=... [more]
A0A067E5M0_CITSI4.5e-2242.36Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g003281mg PE=4 SV=1[more]
A0A151THH2_CAJCA4.5e-2250.85Replication protein A 70 kDa DNA-binding subunit OS=Cajanus cajan GN=KK1_012785 ... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
gi|659095462|ref|XP_008448593.1|1.5e-5574.83PREDICTED: replication protein A 70 kDa DNA-binding subunit A [Cucumis melo][more]
gi|449456771|ref|XP_004146122.1|2.0e-5574.83PREDICTED: replication protein A 70 kDa DNA-binding subunit A [Cucumis sativus][more]
gi|147856620|emb|CAN82475.1|1.5e-2646.90hypothetical protein VITISV_038295 [Vitis vinifera][more]
gi|225427514|ref|XP_002264009.1|1.9e-2646.90PREDICTED: replication protein A 70 kDa DNA-binding subunit A [Vitis vinifera][more]
gi|296088465|emb|CBI37456.3|1.9e-2646.90unnamed protein product [Vitis vinifera][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001878Znf_CCHC
Vocabulary: Molecular Function
TermDefinition
GO:0003676nucleic acid binding
GO:0008270zinc ion binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006310 DNA recombination
biological_process GO:0006281 DNA repair
biological_process GO:0006260 DNA replication
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003676 nucleic acid binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G012150.1CmaCh16G012150.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001878Zinc finger, CCHC-typeGENE3DG3DSA:4.10.60.10coord: 67..130
score: 3.4
IPR001878Zinc finger, CCHC-typePFAMPF00098zf-CCHCcoord: 115..130
score: 2.
IPR001878Zinc finger, CCHC-typeSMARTSM00343c2hcfinal6coord: 115..131
score: 1.2E-4coord: 71..87
score: 0
IPR001878Zinc finger, CCHC-typePROFILEPS50158ZF_CCHCcoord: 72..87
score: 8.565coord: 116..130
score: 10
IPR001878Zinc finger, CCHC-typeunknownSSF57756Retrovirus zinc finger-like domainscoord: 98..135
score: 1.07