CmaCh16G011260 (gene) Cucurbita maxima (Rimu)

NameCmaCh16G011260
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionMethionyl-tRNA formyltransferase
LocationCma_Chr16 : 8668725 .. 8672504 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCCTCATTCGGAGAGTTTCGTCGGCTTTCCCTCGAGTTTTTGCGTTTGCGAATAGGTCCTTGTATTCTTTGAATTCTCCGGGCGAGTCCTTGGATTTACCGACCTCTTCCTCTTTCACTCATGGAATCCATGTCTTCCATTGTCCTGTAAGTTCTCCTTCGATTGCTGACTAAGTTAGACTGCTTCGACTCTGTGTTCTATTCTAATATAAATGTCTCTATTATCGGATATAAAGACGAAGGATAGATTGCTTGTGCGATTTATACTGGTTTTGTGATAGGATGCCATGGGAATCGTTGCTAAGCTATCGGAATGTATAGCTTCCAGAGGGGGAAACATACTCGGCGCCGATATTTTTGTCCCTGAAAACAAGAACGTCTTCTATTCCAGAAGGTAATCTATGGCTTCTTTTGCTTATGGTGGTCTCTGAATAAATCATTTCATATTATTGTACTATGTAACTGCCCTTTTCTTATTCTGTGCTTTAAAAGAGTTTTATAGATCATATGAATATAAACATAAGGAAGGAAGCCAAGCTCCAGCTAATAAATAAATCACTCAATTGACACAGAGTTTTGATAATGTAATTGTTAAAAGGAGAGACAAGTTTACATTAAAAAGTAGTCAGTAGATTCAAAAGAGACGCCATCTAGAAATTGTTCCAAGGGTTATCTTTGTTGTTAAAGATCCTGTTAAATGATGTCTCTATCTTATTATGGGTCTTTGTTGAATTAAACAAAAAAGAATATGATCCATCCAGAACCTATCAAGGGGGTTTGTATTTTTGCTATAGATTAAAGAAAGAGGTCTGTTATTATCTTGACATCTACAAAGTCAAATCTCAAGTCAAGGCGTTAGCTCTTTAGAAAGATTGTAGGGCATTGAGGCTTGACTAAGAATCTCGAAAGGGCTTTTGAAGCTTACTCTGCTACAGCGAACCGTTAGGTTAGCAGGGTGCCCCGGTTTGGGGGCTTGAAGGACTTAGCGTCATGACAAGGGGTATCTGAGCCAAAGGTTCGGCCATGCCATGGCTAGTGGGGGAAAAAAAAAACCATAGACTAGTGTTGCGAAGAGGCTGGGAGAAGACTCATTGCTCGGCCACAGTATACTTAGCCCCTTAATTTTGTAATCTAATTTACGCAATTACATATAAGGCTAAATTGATCTACTCAACAATTGTCGCATTCAAAGTAAAAGATGTTCTTTTATTTTGTTTCGTGTCTTCCTAGGAAACGTGGATACAAATGAAAGGATAATGATTATGACAGGTTTAATATATGAACTTTACGATTGCTTAACTCGTCAAGCAACATGTTCCATGACCATGTGGTGAGTCAAGAAAGACGTCCATGCTCATATTTCATTTTCTACTGCATTTTTTCTTGTATCTCTAGAAGCCGTGTCCTCCTTTTATTGTATATCAGGATTACTCATAATTTTCAAGAGTGAAATTTGATCTTGTGTTCCTGGCTGTTACTGAGGACGTGCATCAGTAACTGCAATATTATACTGACATAATTGCTGCTTTCTGGGATGTTATTGAAGACTTGCATTAGTAACTACAATTTGAGAGGAACATCATGATTTTACTTTGTTTTTTCCTTTCTGGGAACAGTGGAAGTTCTTACATACTTGATACTCTTAAAAAGCGTTGTTATTGTTGTTTTCTGGGAACAGTGGAAGTTCTTGCATATATGAAAAGCATGCCTAAAAAGCTACCAAACATTTCTCGCGAAGTTTAGAACATTATAACTGCTCTTCTTTGGTATCCATGGAAGTCTTAAGAGTGGTTGATGAGAGAACATTTCTAGTCTTGTGTTCAAGAATGTAATTCATGACTCGCATTTGTAACTTGAAATAAAAGAGTATTCCGATTGTACACATACTGCTCCCTAGTGAGATTATGTATGCTACGCTGTGCACCTGTTTTTTTCTGGAATAAACTAATACAGTTTTTAGTTTTATGGCCATTAACTTGGCTGTGATGAATGCCCTATTGAACTTTATATCTTCAAGGAAGCAATTTGGATTTCTCTAGTGCACTATGCTGAAAGCGAAAACTCTACTTGAAATTCTAGCTTGAACCACCACCTGTGTCTGACTTTCGTTTTATGTGCAGCCAGTTTCTTTTTGATCCTTGTAAATGGCCTAGGATGCAAATGGATGAAGACTTCCTTAAGATTTCCAAAACATTTAACGCTTGGAAATCTGTTGTCCGAGTACCAGATCAAGACCCCAAATATAAGATTGCAATTCTTGCTTCAAAACAGGTATACATATACCTGTCAATTTCATGTCAATCATTTTTGTTTGCATGGATATTTGAGATTAACTTACTTTTCCCATTGCAGGATCATTGTCTGGTTGACTTACTTCATCAATGGCAAGGAGGGAAACTTCCAGTGGAAATAACTTGTGTTATAAGGTCAACCGAAACTTGCTTCTTTAATTTGTTTCGAAGTATCGTGACCTTTCTTCTCAATCCACTTGTTTCAGTAATCACGATAGAGCCCCCAACACCCACGTGATGCGATTTCTTGAAAGGCATGGTATTGCTTATCATTATTTGCACACATACAATGATAATAAAAGAGAAGGAGAGATATTGGAATTGGTGAAGAATACTGATTTTTTAGTACTCGCCAGGTATATGCAGGTAAATTGGATCATAATACCTATGCATTCGTAGTCGTACCAGCTGAATTCTTTAGAAAAAAAATTAGTTAATTATGAAGTTCTGTCTTGTTGAAAAGTAAATCTTAATTCAATTTCTTAGTGTTAATTGATTCATTGAGCAATTCAAGATCTAATCCTGGCAATTATCAAATTTCTTCTGCAGGTATTGTCTGGTAATTTCTTGAGGAGCTACAGAAATGATGTAATTAACATCCACCATGGTCTTCTGCCTTCATTCAAGGGTAGTAATCCTTCAAAACAGGCAAGTTACACAAGCCATCAACTCCCCCTCCCCAACTCCCATTTTGTTTCTTTTTTCTGTAACTGTCAATGCATCTTTTATCTGTTAGATTTCATTGACAGGCGTTTAATGCTGGCGTGAAGTTAATTGGTGCTACAAGCCATTTTGTGACAGAAGAACTCGATGCTGGACCAATCATTGAACAGATGGTATCTTTTAGATTGTTTGATGCAACTAATCTGCATGTTGTCTATTTCCTTGTTCTTTATCTCTGAACATAGCCTGCTCCTCTACTCCTTTTTTCTTTTTATAAATTCTTGGGTGTTTAATTCTTGTTCTTTATTTTAGAACCATGCCTCTCCTCTCCTCCTCTACCCTTCTTTTTTTCTTTTTATAAATTCTTGGATGTTTAATCAAGTCATTCTTAACCCTTGTTTTTCTAAAAAACTATTAAATACGCAGTCCATGGATTGTCCTTGCGAACATGTTGGTTGCCATTTTCTTTTCATTAATATAACCACTAAGTTTTTGTTCAAAATTATCAGGTTGAGAGAGTTTCCCACAGAGATAATTTGCAGAGTTTTGTGCAGAAGTCAGAGACCTTAGAAAAGCAATGTCTAACGAATGCAATTAAATCATACTGTGAGCTGCGCGTTTTGCCATACGAGGAAAACAGGACTGTTGTTTTTTAAGAGGAAAAGGATGCATCCATTTGAAGTGTGTCAAGTACATTTCTTCTTCTGCTCATTTGATTTTTCAATTAACATACTCTTTTTGGCATATTTTATACGTTGAATTCAATTTCAATTTATATATACCTTATAACTCTACCTTGATTTGTTTCAAATCAACTTGCAAG

mRNA sequence

ATGGTCCTCATTCGGAGAGTTTCGTCGGCTTTCCCTCGAGTTTTTGCGTTTGCGAATAGGTCCTTGTATTCTTTGAATTCTCCGGGCGAGTCCTTGGATTTACCGACCTCTTCCTCTTTCACTCATGGAATCCATGTCTTCCATTGTCCTGATGCCATGGGAATCGTTGCTAAGCTATCGGAATGTATAGCTTCCAGAGGGGGAAACATACTCGGCGCCGATATTTTTGTCCCTGAAAACAAGAACGTCTTCTATTCCAGAAGCCAGTTTCTTTTTGATCCTTGTAAATGGCCTAGGATGCAAATGGATGAAGACTTCCTTAAGATTTCCAAAACATTTAACGCTTGGAAATCTGTTGTCCGAGTACCAGATCAAGACCCCAAATATAAGATTGCAATTCTTGCTTCAAAACAGGATCATTGTCTGGTTGACTTACTTCATCAATGGCAAGGAGGGAAACTTCCAGTGGAAATAACTTGTGTTATAAGTAATCACGATAGAGCCCCCAACACCCACGTGATGCGATTTCTTGAAAGGCATGGTATTGCTTATCATTATTTGCACACATACAATGATAATAAAAGAGAAGGAGAGATATTGGAATTGGTGAAGAATACTGATTTTTTAGTACTCGCCAGGTATATGCAGGTATTGTCTGGTAATTTCTTGAGGAGCTACAGAAATGATGTAATTAACATCCACCATGGTCTTCTGCCTTCATTCAAGGGTAGTAATCCTTCAAAACAGGCGTTTAATGCTGGCGTGAAGTTAATTGGTGCTACAAGCCATTTTGTGACAGAAGAACTCGATGCTGGACCAATCATTGAACAGATGGTTGAGAGAGTTTCCCACAGAGATAATTTGCAGAGTTTTGTGCAGAAGTCAGAGACCTTAGAAAAGCAATGTCTAACGAATGCAATTAAATCATACTGTGAGCTGCGCGTTTTGCCATACGAGGAAAACAGGACTGTTGTTTTTTAAGAGGAAAAGGATGCATCCATTTGAAGTGTGTCAAGTACATTTCTTCTTCTGCTCATTTGATTTTTCAATTAACATACTCTTTTTGGCATATTTTATACGTTGAATTCAATTTCAATTTATATATACCTTATAACTCTACCTTGATTTGTTTCAAATCAACTTGCAAG

Coding sequence (CDS)

ATGGTCCTCATTCGGAGAGTTTCGTCGGCTTTCCCTCGAGTTTTTGCGTTTGCGAATAGGTCCTTGTATTCTTTGAATTCTCCGGGCGAGTCCTTGGATTTACCGACCTCTTCCTCTTTCACTCATGGAATCCATGTCTTCCATTGTCCTGATGCCATGGGAATCGTTGCTAAGCTATCGGAATGTATAGCTTCCAGAGGGGGAAACATACTCGGCGCCGATATTTTTGTCCCTGAAAACAAGAACGTCTTCTATTCCAGAAGCCAGTTTCTTTTTGATCCTTGTAAATGGCCTAGGATGCAAATGGATGAAGACTTCCTTAAGATTTCCAAAACATTTAACGCTTGGAAATCTGTTGTCCGAGTACCAGATCAAGACCCCAAATATAAGATTGCAATTCTTGCTTCAAAACAGGATCATTGTCTGGTTGACTTACTTCATCAATGGCAAGGAGGGAAACTTCCAGTGGAAATAACTTGTGTTATAAGTAATCACGATAGAGCCCCCAACACCCACGTGATGCGATTTCTTGAAAGGCATGGTATTGCTTATCATTATTTGCACACATACAATGATAATAAAAGAGAAGGAGAGATATTGGAATTGGTGAAGAATACTGATTTTTTAGTACTCGCCAGGTATATGCAGGTATTGTCTGGTAATTTCTTGAGGAGCTACAGAAATGATGTAATTAACATCCACCATGGTCTTCTGCCTTCATTCAAGGGTAGTAATCCTTCAAAACAGGCGTTTAATGCTGGCGTGAAGTTAATTGGTGCTACAAGCCATTTTGTGACAGAAGAACTCGATGCTGGACCAATCATTGAACAGATGGTTGAGAGAGTTTCCCACAGAGATAATTTGCAGAGTTTTGTGCAGAAGTCAGAGACCTTAGAAAAGCAATGTCTAACGAATGCAATTAAATCATACTGTGAGCTGCGCGTTTTGCCATACGAGGAAAACAGGACTGTTGTTTTTTAA

Protein sequence

MVLIRRVSSAFPRVFAFANRSLYSLNSPGESLDLPTSSSFTHGIHVFHCPDAMGIVAKLSECIASRGGNILGADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWKSVVRVPDQDPKYKIAILASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFLERHGIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSYRNDVINIHHGLLPSFKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSETLEKQCLTNAIKSYCELRVLPYEENRTVVF
BLAST of CmaCh16G011260 vs. Swiss-Prot
Match: PURU2_ARATH (Formyltetrahydrofolate deformylase 2, mitochondrial OS=Arabidopsis thaliana GN=PURU2 PE=1 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 1.6e-137
Identity = 242/328 (73.78%), Postives = 274/328 (83.54%), Query Frame = 1

Query: 3   LIRRVSS-AFPRVFAFANRSLYSLNSP---GESLDLPTSSSFTHGIHVFHCPDAMGIVAK 62
           +IRRVS+ +     AF + + +S  S    GESLD   S     G HVFHCPD +GIVAK
Sbjct: 1   MIRRVSTTSCLSATAFRSFTKWSFKSSQFHGESLDSSVSPLLIPGFHVFHCPDVVGIVAK 60

Query: 63  LSECIASRGGNILGADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWKS 122
           LS+CIA++GGNILG D+FVPENKNVFYSRS+F+FDP KWPR QMDEDF  I++ F+A  S
Sbjct: 61  LSDCIAAKGGNILGYDVFVPENKNVFYSRSEFIFDPVKWPRRQMDEDFQTIAQKFSALSS 120

Query: 123 VVRVPDQDPKYKIAILASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFLE 182
           VVRVP  DPKYKIA+L SKQDHCLV++LH+WQ GKLPV+ITCVISNH+RAPNTHVMRFL+
Sbjct: 121 VVRVPSLDPKYKIALLLSKQDHCLVEMLHKWQDGKLPVDITCVISNHERAPNTHVMRFLQ 180

Query: 183 RHGIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSYRNDVINIHHGLL 242
           RHGI+YHYL T + NK E EILELVK TDFLVLARYMQ+LSGNFL+ Y  DVINIHHGLL
Sbjct: 181 RHGISYHYLPTTDQNKIEEEILELVKGTDFLVLARYMQLLSGNFLKGYGKDVINIHHGLL 240

Query: 243 PSFKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSETL 302
           PSFKG NP KQAF+AGVKLIGAT+HFVTEELD+GPIIEQMVERVSHRDNL+SFVQKSE L
Sbjct: 241 PSFKGRNPVKQAFDAGVKLIGATTHFVTEELDSGPIIEQMVERVSHRDNLRSFVQKSEDL 300

Query: 303 EKQCLTNAIKSYCELRVLPYEENRTVVF 327
           EK+CL  AIKSYCELRVLPY   RTVVF
Sbjct: 301 EKKCLMKAIKSYCELRVLPYGTQRTVVF 328

BLAST of CmaCh16G011260 vs. Swiss-Prot
Match: PURU1_ARATH (Formyltetrahydrofolate deformylase 1, mitochondrial OS=Arabidopsis thaliana GN=PURU1 PE=1 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 8.7e-136
Identity = 237/326 (72.70%), Postives = 270/326 (82.82%), Query Frame = 1

Query: 3   LIRRVSSAFPRVFAFANRS--LYSLNSPGESLDLPTSSSFTHGIHVFHCPDAMGIVAKLS 62
           +IRR++    R   FA     L S    GESLD   S     G+HVFHC DA+GIVAKLS
Sbjct: 1   MIRRITE---RASGFAKNIPILKSSRFHGESLDSSVSPVLIPGVHVFHCQDAVGIVAKLS 60

Query: 63  ECIASRGGNILGADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWKSVV 122
           +CIA++GGNILG D+FVPEN NVFYSRS+F+FDP KWPR Q+DEDF  I++ + A  SVV
Sbjct: 61  DCIAAKGGNILGYDVFVPENNNVFYSRSEFIFDPVKWPRSQVDEDFQTIAQRYGALNSVV 120

Query: 123 RVPDQDPKYKIAILASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFLERH 182
           RVP  DPKYKIA+L SKQDHCLV++LH+WQ GKLPV+ITCVISNH+RA NTHVMRFLERH
Sbjct: 121 RVPSIDPKYKIALLLSKQDHCLVEMLHKWQDGKLPVDITCVISNHERASNTHVMRFLERH 180

Query: 183 GIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSYRNDVINIHHGLLPS 242
           GI YHY+ T  +NKRE +ILELVK+TDFLVLARYMQ+LSGNFL+ Y  DVINIHHGLLPS
Sbjct: 181 GIPYHYVSTTKENKREDDILELVKDTDFLVLARYMQILSGNFLKGYGKDVINIHHGLLPS 240

Query: 243 FKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSETLEK 302
           FKG  P+KQAF+AGVKLIGATSHFVTEELD+GPIIEQMVE VSHRDNL+SFVQKSE LEK
Sbjct: 241 FKGGYPAKQAFDAGVKLIGATSHFVTEELDSGPIIEQMVESVSHRDNLRSFVQKSEDLEK 300

Query: 303 QCLTNAIKSYCELRVLPYEENRTVVF 327
           +CLT AIKSYCELRVLPY  N+TVVF
Sbjct: 301 KCLTRAIKSYCELRVLPYGTNKTVVF 323

BLAST of CmaCh16G011260 vs. Swiss-Prot
Match: PURU_BACSU (Formyltetrahydrofolate deformylase OS=Bacillus subtilis (strain 168) GN=purU PE=3 SV=2)

HSP 1 Score: 199.9 bits (507), Expect = 4.3e-50
Identity = 111/283 (39.22%), Postives = 170/283 (60.07%), Query Frame = 1

Query: 49  CPDAMGIVAKLSECIASRGGNILGADIFVPENKNV-FYSRSQFLFDPCKWPRMQMDEDFL 108
           CPD  GIV+ +S  +   G NI+ ++ +  + +   F+ R +F     +  +  +   F 
Sbjct: 26  CPDQPGIVSAVSAFLFEHGANIIESNQYTTDPEGGRFFLRIEFDCAGIREKKSSLQAAFA 85

Query: 109 KISKTFNAWKSVVRVPDQDPKYKIAILASKQDHCLVDLLHQWQGGKLPVEITCVISNHDR 168
            +++ F+   S+    +     ++AI  SK+ HCL +L+ +WQ G L  EI  VISNH+ 
Sbjct: 86  SVAEKFDMTWSLTLASELK---RVAIFVSKELHCLHELIWEWQTGNLMAEIAVVISNHEE 145

Query: 169 APNTHVMRFLERHGIAYHYLHTYNDNKREGEI--LELVK--NTDFLVLARYMQVLSGNFL 228
           A        +ER  I +HY+    D + E E   LEL++  + D +VLARYMQ+L+ +F+
Sbjct: 146 A-----RELVERLNIPFHYMKANKDIRAEVEKKQLELLEQYDVDVIVLARYMQILTPDFV 205

Query: 229 RSYRNDVINIHHGLLPSFKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVS 288
            ++ N +INIHH  LP+F G+NP K+A+  GVKLIGATSH+VT +LD GPIIEQ +ERV 
Sbjct: 206 SAHPNRIINIHHSFLPAFIGANPYKRAYERGVKLIGATSHYVTNDLDEGPIIEQDIERVD 265

Query: 289 HRDNLQSFVQKSETLEKQCLTNAIKSYCELRVLPYEENRTVVF 327
           HRDN ++      T+E+  L  A+K + E RV+ + EN+T+VF
Sbjct: 266 HRDNAEALKNIGRTIERSVLARAVKWHLEDRVIVH-ENKTIVF 299

BLAST of CmaCh16G011260 vs. Swiss-Prot
Match: PURU_SYNY3 (Formyltetrahydrofolate deformylase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=purU PE=3 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.6e-49
Identity = 110/283 (38.87%), Postives = 170/283 (60.07%), Query Frame = 1

Query: 49  CPDAMGIVAKLSECIASRGGNILGADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLK 108
           CPD  GIVA++++ I    GNI+ AD     +  +F +R ++  D  +  R ++   + +
Sbjct: 12  CPDQPGIVAQIAQFIYQNQGNIIHADQHTDFSSGLFLNRVEWQLDNFRLSRPELLSAWSQ 71

Query: 109 ISKTFNA-WKSVVRVPDQDPKYKIAILASKQDHCLVDLLHQWQGGKLPVEITCVISNHDR 168
           +++   A W+  +   DQ P+  +A+  SKQDHCL+D+L +W+ G+L  EI  +ISNH  
Sbjct: 72  LAEQLQATWQ--IHFSDQLPR--LALWVSKQDHCLLDILWRWRSGELRCEIPLIISNHP- 131

Query: 169 APNTHVMRFLERHGIAYHYLHTYNDNKREGEILELVK----NTDFLVLARYMQVLSGNFL 228
                +    ++ GI +H L    +NK   E  EL        D +VLA+Y+Q+L+ +F+
Sbjct: 132 ----DLKSIADQFGIDFHCLPITKENKLAQETAELALLKQYQIDLVVLAKYLQILTTDFV 191

Query: 229 RSYRNDVINIHHGLLPSFKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVS 288
             + N +INIHH  LP+F G+NP  +A   GVK+IGAT+H+ T +LD GPIIEQ V RVS
Sbjct: 192 VQFPN-IINIHHSFLPAFPGANPYHRAHERGVKIIGATAHYATAQLDEGPIIEQDVVRVS 251

Query: 289 HRDNLQSFVQKSETLEKQCLTNAIKSYCELRVLPYEENRTVVF 327
           HRDN+   ++K   LE+  L  A++ + + R+L Y +NRTVVF
Sbjct: 252 HRDNVDDLIRKGRDLERVVLARAVRLHLQHRILVY-DNRTVVF 283

BLAST of CmaCh16G011260 vs. Swiss-Prot
Match: PURU_CORS1 (Formyltetrahydrofolate deformylase OS=Corynebacterium sp. (strain P-1) GN=purU PE=3 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 2.0e-39
Identity = 102/282 (36.17%), Postives = 149/282 (52.84%), Query Frame = 1

Query: 49  CPDAMGIVAKLSECIASRGGNILGADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLK 108
           CP+ +GIV  ++  +      I+    +   +    + R  F  D        +  +F +
Sbjct: 13  CPEGIGIVHAVTGFLVRHQRTIVELKQYDDMSAGRLFLRVDFAGDSAPDLLDALRSEFSE 72

Query: 109 ISKTFNA-WKSVVRVPDQDPKYKIAILASKQDHCLVDLLHQWQGGKLPVEITCVISNHDR 168
           ++  F+  W+    + ++  K K+ I+ SK +HCL DLL +   G LP+E+  V SNH  
Sbjct: 73  VAAKFDMDWQ----LRERGQKTKVLIMVSKFEHCLQDLLFRMHSGDLPIEVVGVASNH-- 132

Query: 169 APNTHVMRFLERHGIAYHYLHTYNDNKREGE--ILELVKNT--DFLVLARYMQVLSGNFL 228
            P+   +  +E +GI +H++    D K   E  +LEL+  T  + +VLARYMQVLS +  
Sbjct: 133 -PDHRSL--VEWYGIGFHHIPISKDTKPRAEAALLELIDQTGAELVVLARYMQVLSDHLA 192

Query: 229 RSYRNDVINIHHGLLPSFKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVS 288
                  INIHH  LPSFKG+ P  QA+  GVK +GAT+H+V  ELD GPII Q V  V 
Sbjct: 193 SELTGKTINIHHSFLPSFKGAKPYHQAWERGVKTVGATAHYVNSELDEGPIIAQQVVEVD 252

Query: 289 HRDNLQSFVQKSETLEKQCLTNAIKSYCELRVLPYEENRTVV 326
           H    Q  V      E + L+NA++ +CE RV  Y  NRTVV
Sbjct: 253 HTYGPQDLVAAGRDSECKALSNAVRWHCEGRVFLY-GNRTVV 284

BLAST of CmaCh16G011260 vs. TrEMBL
Match: A0A0A0L1I1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G019900 PE=4 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 3.3e-182
Identity = 312/326 (95.71%), Postives = 317/326 (97.24%), Query Frame = 1

Query: 1   MVLIRRVSSAFPRVFAFANRSLYSLNSPGESLDLPTSSSFTHGIHVFHCPDAMGIVAKLS 60
           M LIRRVSS+FPRVF FANRSL SLNSPGE+LDLPTSSSF HGIHVFHCPDA+GIVAKLS
Sbjct: 1   MALIRRVSSSFPRVFGFANRSLSSLNSPGEALDLPTSSSFAHGIHVFHCPDAVGIVAKLS 60

Query: 61  ECIASRGGNILGADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWKSVV 120
           ECIAS+GGNIL ADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWKSVV
Sbjct: 61  ECIASKGGNILNADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWKSVV 120

Query: 121 RVPDQDPKYKIAILASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFLERH 180
           RVPDQDPKYKIAI ASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFLERH
Sbjct: 121 RVPDQDPKYKIAIFASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFLERH 180

Query: 181 GIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSYRNDVINIHHGLLPS 240
            IAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSY NDVINIHHGLLPS
Sbjct: 181 DIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSYGNDVINIHHGLLPS 240

Query: 241 FKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSETLEK 300
           FKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSE LEK
Sbjct: 241 FKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSENLEK 300

Query: 301 QCLTNAIKSYCELRVLPYEENRTVVF 327
           QCLTNAI+SYCELRVLPYEENRTVVF
Sbjct: 301 QCLTNAIRSYCELRVLPYEENRTVVF 326

BLAST of CmaCh16G011260 vs. TrEMBL
Match: D7U3P4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0229g00160 PE=4 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 1.6e-149
Identity = 258/329 (78.42%), Postives = 286/329 (86.93%), Query Frame = 1

Query: 1   MVLIRRVSSAFPRVFAFANRSLYSLNSPGESL---DLPTSSSFTHGIHVFHCPDAMGIVA 60
           M L RR+S++ P++  F NRS  SL  P +SL   +  + SS  HGIHVFHCPD +GIVA
Sbjct: 1   MSLRRRLSTSLPQLLGFTNRSFKSLKFPQQSLHCFNSSSESSLAHGIHVFHCPDGVGIVA 60

Query: 61  KLSECIASRGGNILGADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWK 120
           KLS+CIASRGGNILGADIFVPENK VFYSRS+F+FDP KWPR QMDEDFLK+S  FNA K
Sbjct: 61  KLSDCIASRGGNILGADIFVPENKKVFYSRSEFIFDPAKWPRAQMDEDFLKLSNMFNAMK 120

Query: 121 SVVRVPDQDPKYKIAILASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFL 180
           SVVRVP  DPKYKI++LASKQDHCLVDLLH WQ G+LPV+ITCVISNHDR PNTHV RFL
Sbjct: 121 SVVRVPILDPKYKISVLASKQDHCLVDLLHGWQDGRLPVDITCVISNHDRGPNTHVFRFL 180

Query: 181 ERHGIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSYRNDVINIHHGL 240
           ERHGI YHYLHT  +NKREGEIL+LV++TDFLVLARYMQ+LSGNFL+SY  D+INIHHGL
Sbjct: 181 ERHGIPYHYLHTTKENKREGEILDLVQDTDFLVLARYMQILSGNFLKSYGKDIINIHHGL 240

Query: 241 LPSFKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSET 300
           LPSFKG NPSKQAF+AGVKLIGATSHFVTEELDAGPII QMVERV HRDNL+SFVQKSE 
Sbjct: 241 LPSFKGGNPSKQAFDAGVKLIGATSHFVTEELDAGPIIGQMVERVCHRDNLKSFVQKSEN 300

Query: 301 LEKQCLTNAIKSYCELRVLPYEENRTVVF 327
           LEKQCL  AIKSYCELRVLPYE+N+TVVF
Sbjct: 301 LEKQCLVKAIKSYCELRVLPYEDNKTVVF 329

BLAST of CmaCh16G011260 vs. TrEMBL
Match: M5WB64_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008574mg PE=4 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 8.1e-149
Identity = 255/326 (78.22%), Postives = 286/326 (87.73%), Query Frame = 1

Query: 1   MVLIRRVSSAFPRVFAFANRSLYSLNSPGESLDLPTSSSFTHGIHVFHCPDAMGIVAKLS 60
           M L++R S + P++F FA RS  SL  PGE LD  +S   ++GIHVFH PDA+GIVAKLS
Sbjct: 1   MSLVQRASPSLPKLFGFAKRSFKSLRFPGEPLDPSSSPILSYGIHVFHAPDAVGIVAKLS 60

Query: 61  ECIASRGGNILGADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWKSVV 120
           +CIAS+GGNILGA +FVPENK+VFYSRS+F+FDP KWPR +MDEDF K+  T++A +SVV
Sbjct: 61  DCIASKGGNILGASVFVPENKHVFYSRSEFIFDPIKWPRSEMDEDFRKLLTTYSAMRSVV 120

Query: 121 RVPDQDPKYKIAILASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFLERH 180
           RVPD DPKYKI ILASKQDHCLVDLLHQWQ G+LPV+ITCVISNHDR PNTHV RFLERH
Sbjct: 121 RVPDHDPKYKICILASKQDHCLVDLLHQWQEGRLPVDITCVISNHDRGPNTHVSRFLERH 180

Query: 181 GIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSYRNDVINIHHGLLPS 240
           GI YHYL T N +KREGEILELV+NTDFLVLARYMQ+LSG+FL+SY  DVINIHHGLLPS
Sbjct: 181 GIPYHYLQTTNTDKREGEILELVQNTDFLVLARYMQLLSGDFLKSYGKDVINIHHGLLPS 240

Query: 241 FKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSETLEK 300
           FKG  PSKQAF+AGVKLIGATSHFVT+ELDAGPIIEQMV RVSHRDNLQSFVQKSE LEK
Sbjct: 241 FKGGKPSKQAFDAGVKLIGATSHFVTQELDAGPIIEQMVARVSHRDNLQSFVQKSENLEK 300

Query: 301 QCLTNAIKSYCELRVLPYEENRTVVF 327
           QCLT AIKSYCELRVLPYE+N+TVVF
Sbjct: 301 QCLTKAIKSYCELRVLPYEDNKTVVF 326

BLAST of CmaCh16G011260 vs. TrEMBL
Match: A0A0D2N0Z3_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_004G197200 PE=4 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 4.5e-147
Identity = 257/326 (78.83%), Postives = 284/326 (87.12%), Query Frame = 1

Query: 1   MVLIRRVSSAFPRVFAFANRSLYSLNSPGESLDLPTSSSFTHGIHVFHCPDAMGIVAKLS 60
           M L RR+SS+ P  F FA RS  S   PG      +SSS THGIHVFHCPD +GIVAKLS
Sbjct: 1   MALTRRISSSIPHYFGFAERSFKSYKLPGGP---SSSSSLTHGIHVFHCPDQVGIVAKLS 60

Query: 61  ECIASRGGNILGADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWKSVV 120
           E IASRGGNILGADIFVPE KNVFYSR++FLFDP KWPR QMDEDFL +SK F+A +SVV
Sbjct: 61  ERIASRGGNILGADIFVPEKKNVFYSRNEFLFDPVKWPREQMDEDFLNLSKLFSATRSVV 120

Query: 121 RVPDQDPKYKIAILASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFLERH 180
           RVPD DPK+KI ILASKQDHCLVDLLH+WQ G+LPVEITCVISNH+R PNTHV+RFLER+
Sbjct: 121 RVPDLDPKFKIGILASKQDHCLVDLLHRWQEGRLPVEITCVISNHERGPNTHVIRFLERN 180

Query: 181 GIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSYRNDVINIHHGLLPS 240
            I YHYL+T  +NKREGE+LELV +TDFLVLARYMQ+LSGNFLR+Y  DVINIHHGLLPS
Sbjct: 181 NIPYHYLNTTKENKREGEMLELVLDTDFLVLARYMQILSGNFLRTYGKDVINIHHGLLPS 240

Query: 241 FKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSETLEK 300
           FKG NPSKQAF+AGVKLIGATSHFVTEELD+GPIIEQMVERVSHRDNL+SFVQKSE LE+
Sbjct: 241 FKGGNPSKQAFDAGVKLIGATSHFVTEELDSGPIIEQMVERVSHRDNLESFVQKSENLER 300

Query: 301 QCLTNAIKSYCELRVLPYEENRTVVF 327
           QCL  A++SYCELRVLPYEENRTVVF
Sbjct: 301 QCLAKAMRSYCELRVLPYEENRTVVF 323

BLAST of CmaCh16G011260 vs. TrEMBL
Match: V4VL95_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10020835mg PE=4 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 2.2e-146
Identity = 253/326 (77.61%), Postives = 282/326 (86.50%), Query Frame = 1

Query: 1   MVLIRRVSSAFPRVFAFANRSLYSLNSPGESLDLPTSSSFTHGIHVFHCPDAMGIVAKLS 60
           M L+RR+SS+  +V  F NRS  SL  PGE ++   SS+ THGIHVFHCPD +GIVAKLS
Sbjct: 1   MTLLRRLSSSLQQVVKFTNRSFKSLKFPGEPIESSVSSTLTHGIHVFHCPDEVGIVAKLS 60

Query: 61  ECIASRGGNILGADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWKSVV 120
           ECIASRGGNIL AD+FVPE KNVFYSRS+F+FDP KWPR QMDEDF K+SK FNA +SVV
Sbjct: 61  ECIASRGGNILAADVFVPEKKNVFYSRSEFIFDPIKWPREQMDEDFFKLSKMFNAMRSVV 120

Query: 121 RVPDQDPKYKIAILASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFLERH 180
           RVPD DPKYK+A+LASKQ+HCLVD L+ WQ GKLPVEITCVISNHDR PN+HV+R LERH
Sbjct: 121 RVPDIDPKYKVAVLASKQEHCLVDFLYGWQEGKLPVEITCVISNHDRGPNSHVIRLLERH 180

Query: 181 GIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSYRNDVINIHHGLLPS 240
           GI YHYL    +NKRE E+LELV+NTDFLVLARYMQ+LSG FLRSY  DVINIHHGLLPS
Sbjct: 181 GIPYHYL-CAKENKREEELLELVQNTDFLVLARYMQILSGKFLRSYGKDVINIHHGLLPS 240

Query: 241 FKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSETLEK 300
           FKG  P+KQAF+AGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNL++FVQKSE +EK
Sbjct: 241 FKGGKPAKQAFDAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLRTFVQKSEDVEK 300

Query: 301 QCLTNAIKSYCELRVLPYEENRTVVF 327
           QCL  AIKSYCELRVLPYE N+TVVF
Sbjct: 301 QCLAKAIKSYCELRVLPYEMNKTVVF 325

BLAST of CmaCh16G011260 vs. TAIR10
Match: AT4G17360.1 (AT4G17360.1 Formyl transferase)

HSP 1 Score: 490.3 bits (1261), Expect = 8.9e-139
Identity = 242/328 (73.78%), Postives = 274/328 (83.54%), Query Frame = 1

Query: 3   LIRRVSS-AFPRVFAFANRSLYSLNSP---GESLDLPTSSSFTHGIHVFHCPDAMGIVAK 62
           +IRRVS+ +     AF + + +S  S    GESLD   S     G HVFHCPD +GIVAK
Sbjct: 1   MIRRVSTTSCLSATAFRSFTKWSFKSSQFHGESLDSSVSPLLIPGFHVFHCPDVVGIVAK 60

Query: 63  LSECIASRGGNILGADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWKS 122
           LS+CIA++GGNILG D+FVPENKNVFYSRS+F+FDP KWPR QMDEDF  I++ F+A  S
Sbjct: 61  LSDCIAAKGGNILGYDVFVPENKNVFYSRSEFIFDPVKWPRRQMDEDFQTIAQKFSALSS 120

Query: 123 VVRVPDQDPKYKIAILASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFLE 182
           VVRVP  DPKYKIA+L SKQDHCLV++LH+WQ GKLPV+ITCVISNH+RAPNTHVMRFL+
Sbjct: 121 VVRVPSLDPKYKIALLLSKQDHCLVEMLHKWQDGKLPVDITCVISNHERAPNTHVMRFLQ 180

Query: 183 RHGIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSYRNDVINIHHGLL 242
           RHGI+YHYL T + NK E EILELVK TDFLVLARYMQ+LSGNFL+ Y  DVINIHHGLL
Sbjct: 181 RHGISYHYLPTTDQNKIEEEILELVKGTDFLVLARYMQLLSGNFLKGYGKDVINIHHGLL 240

Query: 243 PSFKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSETL 302
           PSFKG NP KQAF+AGVKLIGAT+HFVTEELD+GPIIEQMVERVSHRDNL+SFVQKSE L
Sbjct: 241 PSFKGRNPVKQAFDAGVKLIGATTHFVTEELDSGPIIEQMVERVSHRDNLRSFVQKSEDL 300

Query: 303 EKQCLTNAIKSYCELRVLPYEENRTVVF 327
           EK+CL  AIKSYCELRVLPY   RTVVF
Sbjct: 301 EKKCLMKAIKSYCELRVLPYGTQRTVVF 328

BLAST of CmaCh16G011260 vs. TAIR10
Match: AT5G47435.2 (AT5G47435.2 formyltetrahydrofolate deformylase, putative)

HSP 1 Score: 484.6 bits (1246), Expect = 4.9e-137
Identity = 237/326 (72.70%), Postives = 270/326 (82.82%), Query Frame = 1

Query: 3   LIRRVSSAFPRVFAFANRS--LYSLNSPGESLDLPTSSSFTHGIHVFHCPDAMGIVAKLS 62
           +IRR++    R   FA     L S    GESLD   S     G+HVFHC DA+GIVAKLS
Sbjct: 1   MIRRITE---RASGFAKNIPILKSSRFHGESLDSSVSPVLIPGVHVFHCQDAVGIVAKLS 60

Query: 63  ECIASRGGNILGADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWKSVV 122
           +CIA++GGNILG D+FVPEN NVFYSRS+F+FDP KWPR Q+DEDF  I++ + A  SVV
Sbjct: 61  DCIAAKGGNILGYDVFVPENNNVFYSRSEFIFDPVKWPRSQVDEDFQTIAQRYGALNSVV 120

Query: 123 RVPDQDPKYKIAILASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFLERH 182
           RVP  DPKYKIA+L SKQDHCLV++LH+WQ GKLPV+ITCVISNH+RA NTHVMRFLERH
Sbjct: 121 RVPSIDPKYKIALLLSKQDHCLVEMLHKWQDGKLPVDITCVISNHERASNTHVMRFLERH 180

Query: 183 GIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSYRNDVINIHHGLLPS 242
           GI YHY+ T  +NKRE +ILELVK+TDFLVLARYMQ+LSGNFL+ Y  DVINIHHGLLPS
Sbjct: 181 GIPYHYVSTTKENKREDDILELVKDTDFLVLARYMQILSGNFLKGYGKDVINIHHGLLPS 240

Query: 243 FKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSETLEK 302
           FKG  P+KQAF+AGVKLIGATSHFVTEELD+GPIIEQMVE VSHRDNL+SFVQKSE LEK
Sbjct: 241 FKGGYPAKQAFDAGVKLIGATSHFVTEELDSGPIIEQMVESVSHRDNLRSFVQKSEDLEK 300

Query: 303 QCLTNAIKSYCELRVLPYEENRTVVF 327
           +CLT AIKSYCELRVLPY  N+TVVF
Sbjct: 301 KCLTRAIKSYCELRVLPYGTNKTVVF 323

BLAST of CmaCh16G011260 vs. NCBI nr
Match: gi|449458970|ref|XP_004147219.1| (PREDICTED: formyltetrahydrofolate deformylase 1, mitochondrial [Cucumis sativus])

HSP 1 Score: 645.6 bits (1664), Expect = 4.7e-182
Identity = 312/326 (95.71%), Postives = 317/326 (97.24%), Query Frame = 1

Query: 1   MVLIRRVSSAFPRVFAFANRSLYSLNSPGESLDLPTSSSFTHGIHVFHCPDAMGIVAKLS 60
           M LIRRVSS+FPRVF FANRSL SLNSPGE+LDLPTSSSF HGIHVFHCPDA+GIVAKLS
Sbjct: 1   MALIRRVSSSFPRVFGFANRSLSSLNSPGEALDLPTSSSFAHGIHVFHCPDAVGIVAKLS 60

Query: 61  ECIASRGGNILGADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWKSVV 120
           ECIAS+GGNIL ADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWKSVV
Sbjct: 61  ECIASKGGNILNADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWKSVV 120

Query: 121 RVPDQDPKYKIAILASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFLERH 180
           RVPDQDPKYKIAI ASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFLERH
Sbjct: 121 RVPDQDPKYKIAIFASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFLERH 180

Query: 181 GIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSYRNDVINIHHGLLPS 240
            IAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSY NDVINIHHGLLPS
Sbjct: 181 DIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSYGNDVINIHHGLLPS 240

Query: 241 FKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSETLEK 300
           FKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSE LEK
Sbjct: 241 FKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSENLEK 300

Query: 301 QCLTNAIKSYCELRVLPYEENRTVVF 327
           QCLTNAI+SYCELRVLPYEENRTVVF
Sbjct: 301 QCLTNAIRSYCELRVLPYEENRTVVF 326

BLAST of CmaCh16G011260 vs. NCBI nr
Match: gi|659095897|ref|XP_008448821.1| (PREDICTED: formyltetrahydrofolate deformylase 1, mitochondrial [Cucumis melo])

HSP 1 Score: 643.7 bits (1659), Expect = 1.8e-181
Identity = 311/326 (95.40%), Postives = 316/326 (96.93%), Query Frame = 1

Query: 1   MVLIRRVSSAFPRVFAFANRSLYSLNSPGESLDLPTSSSFTHGIHVFHCPDAMGIVAKLS 60
           M LIRRVSS+FPRVF   NRSL SLNSPGESLDLPTSSSF HGIHVFHCPDA+GIVAKLS
Sbjct: 1   MALIRRVSSSFPRVFGLTNRSLNSLNSPGESLDLPTSSSFAHGIHVFHCPDAVGIVAKLS 60

Query: 61  ECIASRGGNILGADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWKSVV 120
           ECIAS+GGNILGADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWKSVV
Sbjct: 61  ECIASKGGNILGADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWKSVV 120

Query: 121 RVPDQDPKYKIAILASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFLERH 180
           RVPDQDPKYKIAI ASKQDHCLVDLLHQWQGGKLPVEITCVISNHDR PNTHVMRFLERH
Sbjct: 121 RVPDQDPKYKIAIFASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRDPNTHVMRFLERH 180

Query: 181 GIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSYRNDVINIHHGLLPS 240
           GIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSG+FLRSY NDVINIHHGLLPS
Sbjct: 181 GIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGDFLRSYGNDVINIHHGLLPS 240

Query: 241 FKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSETLEK 300
           FKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSE LEK
Sbjct: 241 FKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSENLEK 300

Query: 301 QCLTNAIKSYCELRVLPYEENRTVVF 327
           QCLTNAI+SYCELRVLPYEENRTVVF
Sbjct: 301 QCLTNAIRSYCELRVLPYEENRTVVF 326

BLAST of CmaCh16G011260 vs. NCBI nr
Match: gi|1009173665|ref|XP_015867932.1| (PREDICTED: formyltetrahydrofolate deformylase 1, mitochondrial-like isoform X2 [Ziziphus jujuba])

HSP 1 Score: 543.1 bits (1398), Expect = 3.3e-151
Identity = 262/328 (79.88%), Postives = 291/328 (88.72%), Query Frame = 1

Query: 1   MVLIRRVSSA--FPRVFAFANRSLYSLNSPGESLDLPTSSSFTHGIHVFHCPDAMGIVAK 60
           M ++RR +S     ++  FANRS  SL  PGES D  + SS T+GIHVFHCPDA+GIVAK
Sbjct: 1   MTVVRRAASPNNLSKLVGFANRSFKSLRFPGESFDSSSPSSVTYGIHVFHCPDAVGIVAK 60

Query: 61  LSECIASRGGNILGADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWKS 120
           LS+CIASRGGNILGAD+FVPE K+VFYSRS+F+FDP KW R+QMDEDF K+SKTFNA +S
Sbjct: 61  LSDCIASRGGNILGADVFVPEKKHVFYSRSEFIFDPLKWSRLQMDEDFSKLSKTFNAIRS 120

Query: 121 VVRVPDQDPKYKIAILASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFLE 180
           VVRVPD+DPKYKIAI ASKQDHCLVDLLHQWQ G+LPVEITCVISNHDR PNTHV+RFLE
Sbjct: 121 VVRVPDRDPKYKIAIFASKQDHCLVDLLHQWQDGRLPVEITCVISNHDRDPNTHVIRFLE 180

Query: 181 RHGIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSYRNDVINIHHGLL 240
           RHGI YHYL T  D+K EG++LELV+NTDFLVLARYMQ+LSGNFL+SY  DVINIHHGLL
Sbjct: 181 RHGIPYHYLCTNKDDKSEGQMLELVQNTDFLVLARYMQILSGNFLKSYGKDVINIHHGLL 240

Query: 241 PSFKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSETL 300
           PSFKG +PSKQAF+AGVKLIGATSHFVTEELD GPIIEQMVERVSHRDNLQSFVQKSE L
Sbjct: 241 PSFKGGHPSKQAFDAGVKLIGATSHFVTEELDEGPIIEQMVERVSHRDNLQSFVQKSENL 300

Query: 301 EKQCLTNAIKSYCELRVLPYEENRTVVF 327
           EKQCL+ AIKSYCELRVLPYEEN+TVVF
Sbjct: 301 EKQCLSKAIKSYCELRVLPYEENKTVVF 328

BLAST of CmaCh16G011260 vs. NCBI nr
Match: gi|1009173663|ref|XP_015867931.1| (PREDICTED: formyltetrahydrofolate deformylase 1, mitochondrial-like isoform X1 [Ziziphus jujuba])

HSP 1 Score: 538.5 bits (1386), Expect = 8.1e-150
Identity = 262/329 (79.64%), Postives = 291/329 (88.45%), Query Frame = 1

Query: 1   MVLIRRVSSA--FPRVFAFANRSLYSLNSPGESLDLPTSSSFTHGIHVFHCPDAMGIVAK 60
           M ++RR +S     ++  FANRS  SL  PGES D  + SS T+GIHVFHCPDA+GIVAK
Sbjct: 1   MTVVRRAASPNNLSKLVGFANRSFKSLRFPGESFDSSSPSSVTYGIHVFHCPDAVGIVAK 60

Query: 61  LSECIASRGGNILGADIFVPENKNVFYSRS-QFLFDPCKWPRMQMDEDFLKISKTFNAWK 120
           LS+CIASRGGNILGAD+FVPE K+VFYSRS +F+FDP KW R+QMDEDF K+SKTFNA +
Sbjct: 61  LSDCIASRGGNILGADVFVPEKKHVFYSRSSEFIFDPLKWSRLQMDEDFSKLSKTFNAIR 120

Query: 121 SVVRVPDQDPKYKIAILASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFL 180
           SVVRVPD+DPKYKIAI ASKQDHCLVDLLHQWQ G+LPVEITCVISNHDR PNTHV+RFL
Sbjct: 121 SVVRVPDRDPKYKIAIFASKQDHCLVDLLHQWQDGRLPVEITCVISNHDRDPNTHVIRFL 180

Query: 181 ERHGIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSYRNDVINIHHGL 240
           ERHGI YHYL T  D+K EG++LELV+NTDFLVLARYMQ+LSGNFL+SY  DVINIHHGL
Sbjct: 181 ERHGIPYHYLCTNKDDKSEGQMLELVQNTDFLVLARYMQILSGNFLKSYGKDVINIHHGL 240

Query: 241 LPSFKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSET 300
           LPSFKG +PSKQAF+AGVKLIGATSHFVTEELD GPIIEQMVERVSHRDNLQSFVQKSE 
Sbjct: 241 LPSFKGGHPSKQAFDAGVKLIGATSHFVTEELDEGPIIEQMVERVSHRDNLQSFVQKSEN 300

Query: 301 LEKQCLTNAIKSYCELRVLPYEENRTVVF 327
           LEKQCL+ AIKSYCELRVLPYEEN+TVVF
Sbjct: 301 LEKQCLSKAIKSYCELRVLPYEENKTVVF 329

BLAST of CmaCh16G011260 vs. NCBI nr
Match: gi|359495984|ref|XP_003635126.1| (PREDICTED: formyltetrahydrofolate deformylase 1, mitochondrial isoform X1 [Vitis vinifera])

HSP 1 Score: 537.0 bits (1382), Expect = 2.4e-149
Identity = 258/329 (78.42%), Postives = 286/329 (86.93%), Query Frame = 1

Query: 1   MVLIRRVSSAFPRVFAFANRSLYSLNSPGESL---DLPTSSSFTHGIHVFHCPDAMGIVA 60
           M L RR+S++ P++  F NRS  SL  P +SL   +  + SS  HGIHVFHCPD +GIVA
Sbjct: 1   MSLRRRLSTSLPQLLGFTNRSFKSLKFPQQSLHCFNSSSESSLAHGIHVFHCPDGVGIVA 60

Query: 61  KLSECIASRGGNILGADIFVPENKNVFYSRSQFLFDPCKWPRMQMDEDFLKISKTFNAWK 120
           KLS+CIASRGGNILGADIFVPENK VFYSRS+F+FDP KWPR QMDEDFLK+S  FNA K
Sbjct: 61  KLSDCIASRGGNILGADIFVPENKKVFYSRSEFIFDPAKWPRAQMDEDFLKLSNMFNAMK 120

Query: 121 SVVRVPDQDPKYKIAILASKQDHCLVDLLHQWQGGKLPVEITCVISNHDRAPNTHVMRFL 180
           SVVRVP  DPKYKI++LASKQDHCLVDLLH WQ G+LPV+ITCVISNHDR PNTHV RFL
Sbjct: 121 SVVRVPILDPKYKISVLASKQDHCLVDLLHGWQDGRLPVDITCVISNHDRGPNTHVFRFL 180

Query: 181 ERHGIAYHYLHTYNDNKREGEILELVKNTDFLVLARYMQVLSGNFLRSYRNDVINIHHGL 240
           ERHGI YHYLHT  +NKREGEIL+LV++TDFLVLARYMQ+LSGNFL+SY  D+INIHHGL
Sbjct: 181 ERHGIPYHYLHTTKENKREGEILDLVQDTDFLVLARYMQILSGNFLKSYGKDIINIHHGL 240

Query: 241 LPSFKGSNPSKQAFNAGVKLIGATSHFVTEELDAGPIIEQMVERVSHRDNLQSFVQKSET 300
           LPSFKG NPSKQAF+AGVKLIGATSHFVTEELDAGPII QMVERV HRDNL+SFVQKSE 
Sbjct: 241 LPSFKGGNPSKQAFDAGVKLIGATSHFVTEELDAGPIIGQMVERVCHRDNLKSFVQKSEN 300

Query: 301 LEKQCLTNAIKSYCELRVLPYEENRTVVF 327
           LEKQCL  AIKSYCELRVLPYE+N+TVVF
Sbjct: 301 LEKQCLVKAIKSYCELRVLPYEDNKTVVF 329

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PURU2_ARATH1.6e-13773.78Formyltetrahydrofolate deformylase 2, mitochondrial OS=Arabidopsis thaliana GN=P... [more]
PURU1_ARATH8.7e-13672.70Formyltetrahydrofolate deformylase 1, mitochondrial OS=Arabidopsis thaliana GN=P... [more]
PURU_BACSU4.3e-5039.22Formyltetrahydrofolate deformylase OS=Bacillus subtilis (strain 168) GN=purU PE=... [more]
PURU_SYNY31.6e-4938.87Formyltetrahydrofolate deformylase OS=Synechocystis sp. (strain PCC 6803 / Kazus... [more]
PURU_CORS12.0e-3936.17Formyltetrahydrofolate deformylase OS=Corynebacterium sp. (strain P-1) GN=purU P... [more]
Match NameE-valueIdentityDescription
A0A0A0L1I1_CUCSA3.3e-18295.71Uncharacterized protein OS=Cucumis sativus GN=Csa_3G019900 PE=4 SV=1[more]
D7U3P4_VITVI1.6e-14978.42Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0229g00160 PE=4 SV=... [more]
M5WB64_PRUPE8.1e-14978.22Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008574mg PE=4 SV=1[more]
A0A0D2N0Z3_GOSRA4.5e-14778.83Uncharacterized protein OS=Gossypium raimondii GN=B456_004G197200 PE=4 SV=1[more]
V4VL95_9ROSI2.2e-14677.61Uncharacterized protein OS=Citrus clementina GN=CICLE_v10020835mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G17360.18.9e-13973.78 Formyl transferase[more]
AT5G47435.24.9e-13772.70 formyltetrahydrofolate deformylase, putative[more]
Match NameE-valueIdentityDescription
gi|449458970|ref|XP_004147219.1|4.7e-18295.71PREDICTED: formyltetrahydrofolate deformylase 1, mitochondrial [Cucumis sativus][more]
gi|659095897|ref|XP_008448821.1|1.8e-18195.40PREDICTED: formyltetrahydrofolate deformylase 1, mitochondrial [Cucumis melo][more]
gi|1009173665|ref|XP_015867932.1|3.3e-15179.88PREDICTED: formyltetrahydrofolate deformylase 1, mitochondrial-like isoform X2 [... [more]
gi|1009173663|ref|XP_015867931.1|8.1e-15079.64PREDICTED: formyltetrahydrofolate deformylase 1, mitochondrial-like isoform X1 [... [more]
gi|359495984|ref|XP_003635126.1|2.4e-14978.42PREDICTED: formyltetrahydrofolate deformylase 1, mitochondrial isoform X1 [Vitis... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002376Formyl_transf_N
IPR004810PurU
Vocabulary: Molecular Function
TermDefinition
GO:0016742hydroxymethyl-, formyl- and related transferase activity
GO:0008864formyltetrahydrofolate deformylase activity
Vocabulary: Biological Process
TermDefinition
GO:0009058biosynthetic process
GO:0006189'de novo' IMP biosynthetic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009256 10-formyltetrahydrofolate metabolic process
biological_process GO:0006189 'de novo' IMP biosynthetic process
biological_process GO:0046487 glyoxylate metabolic process
biological_process GO:0032259 methylation
biological_process GO:0006144 purine nucleobase metabolic process
biological_process GO:0009058 biosynthetic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016597 amino acid binding
molecular_function GO:0008864 formyltetrahydrofolate deformylase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0004644 phosphoribosylglycinamide formyltransferase activity
molecular_function GO:0016742 hydroxymethyl-, formyl- and related transferase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G011260.1CmaCh16G011260.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002376Formyl transferase, N-terminalGENE3DG3DSA:3.40.50.170coord: 129..321
score: 1.4
IPR002376Formyl transferase, N-terminalPFAMPF00551Formyl_trans_Ncoord: 130..307
score: 4.4
IPR002376Formyl transferase, N-terminalunknownSSF53328Formyltransferasecoord: 130..324
score: 2.88
IPR004810Formyltetrahydrofolate deformylasePRINTSPR01575FFH4HYDRLASEcoord: 152..179
score: 9.2E-28coord: 299..324
score: 9.2E-28coord: 277..299
score: 9.2E-28coord: 130..152
score: 9.2E-28coord: 45..71
score: 9.2
IPR004810Formyltetrahydrofolate deformylasePANTHERPTHR10520:SF7FORMYLTETRAHYDROFOLATE DEFORMYLASE 1, MITOCHONDRIAL-RELATEDcoord: 37..319
score: 9.8E
NoneNo IPR availableGENE3DG3DSA:3.30.70.260coord: 47..114
score: 1.
NoneNo IPR availablePIRPIRSF036480FormyFH4_hydrcoord: 41..326
score: 2.6E
NoneNo IPR availablePANTHERPTHR10520TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3-RELATEDcoord: 37..319
score: 9.8E
NoneNo IPR availableunknownSSF55021ACT-likecoord: 47..115
score: 6.2