CmaCh16G003990 (gene) Cucurbita maxima (Rimu)

NameCmaCh16G003990
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionProbable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1
LocationCma_Chr16 : 1983033 .. 1987548 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGGGCAGTGAAGGTATATCCGTTCTCTATCCTTCTTCTCTGATCTCTGTTGAATCACTCAAATGGCGATGGCTTCACCTCTGGCCGTCAATGGAGTCAAGTTGGCTACGACTTCTCTAGCTTATGTGGACACCAAGGCTGCAAACAATACGAAGGCTCTGATGGCGGAGCTTTCCCGCCAATTCTACGGCCTCCGATTGGTTTCCAGCCCCGCCGGTAGCATTGCCATCAAGGTTCACGACCACTCCATTCCCAAGGGACAACACACAATCTCTTTGTCACATTCCGGTGAGAATTTCTCCATCTTTCTCTTTCTAATTCAGTTCCTTGGGAATGTGATTTTCCTTTAGTCTTTTTGGTGTAGAATTTTTTTTTATTATGGTAATTCCTTCTGCTTCAGTCTTTTTGGCTGTTTGTAACTGCCGTATGAATATGGCTAGGCGTCGTTGTAATTCAAACTAGTTCTTGTTGGGAGGATATCCAATTTACCCTTCTCTTTGCAGAAGAAGATGATCTTAATGGTTCACGCCATCAAGAACTATAGCATAAGATCACCTGTTTTTTTGTTTGGCAAGAGTATAAAGGGCTTTGAATTCACGGATTTAGATGAATTTTCTTTTGCTTTTGTTTGATTATACACAGGTGTTAAGAAGGAGAGAATGCTCGCGGAGGACACCTGCATGTTATCTTATGGTGGGTCAATCTTATATTCACCACCTCCAAAGCCATTTTCTCACAAGCTCCCGAACTGTTTGGATGGTAGACCTCTTTTTATGAAGGTATAGTTCCCTGCATAAGAGTATAATCAACTTGTGATGAATAGAAAATACATAGTTATGCTATAAATGAGCTAATGTTCATATAAGCAATGTCTCCTATACCTCGAGACATTAACTTCACTGATCTTTGCATGATCGACCAACCTTTTTCGTTCTTATTTGATTGCCAAATCCATCCACCACAACCAAATTTGTGTTTCTTATTAAAAACAGCAACAATAACTATTGTTGTGGCATTACTTTAAGTTAGGAGAAGGTTTTGTTTTGTTATTTTGGGTAAATCTCAAACTTATTCAGCATTGTATGTGCCTTTTCTTATCGTCCCGTTTTATTTGATAGAAACCTTCGAATGAGAAGTTCCTTTGAGCTTTGTAGATGATGAAACCTCTGATTTTAAACAGTTTCAAGAAATTGATTTTGGGTCTGTCTTGCTTTCATTCGGTCTCAGTAATCAATAACTTAATTTTACAGGCTGATTGTTGTCACTATCTCTTTGATGCTGCTATTAAGCTTCGTCAACTAGGCATTGATTAGTCTTCTCCAACTCAGGATCCTTTCGGGAGCATCAAAGGAATTTCAAGCAAAATTTCTGTGAAGGCTGGGGCTCAAGGTTTAAATAATGAAAACAAACCATTGCCAGTGAGTTGAAGTGCATTTTGTTTAATTGGGAAGCTTAAAGTTTTAGCCTTTGATTTTTCATTCTTTTCTTTTACCTTTGAGGTATATCATTTTTGTATCTGGGTTAGGAATCTTCTTTTCAATTGTGGTTCATTTCTCATTTATATTTTCGATGCAGCGTTGCATAGTTCTTGATATCGAAGGGACTACTACTCCAATATCTTTTGTTACTGACATACTCTTTCCATATGCTCGTGATAACGTTGGAAAGCATTTGATTTTAACGTATGAAACTGCAGAAACCCAAGATGACATTATGTTATTGCGTTCACAAGTAAGATATTTTGGTTCAACAGTATTTTGATTATGTTGCAGTCTGTAGAATGTCTACGATCTATTTACGGTATTTATTTGAGCATTGTTAGTTAAACAGGAATAGCGTGTGCGTTCATGAGTTAATATGTGATACAGAAATCTCCTCCTACCAGTAAATTCAATTTGTTTGAGATCTCACATCGGTTGGAGAGGAGAACGAAACACCCTTTACAAGGGTGTGGAAATTTCTCCTAGCAGACGTGTTTTAAAAACCTTGAGGGTAAGCCCGAAAGGAAAAGCCCAAAGAGAACAATATCTGCTAGCGGTGGGCTTGGGTTGTTAGAAATGATATTAGAGTCAGACACCAAGCAATGTGACAGCAAGGAGGCTGAGCCCCGAAGGGGGTGGACATGAGGCAATGTGCCAACAAAGATGTTGGGCCCCAAAGGGGTGGATTTAGTGGGGGTCCCACATCGATTGGAGAAAGGAACGAGTGCCAGCGAGGATGCTGGGCTCGGAAGGGGGGTGGATTGTGAGATCTCACATCGGTTGAGGAGGAGAACGAAACGCCTTTTATAAGGGTGTGGAAACTTCTCCTTAGAAGACGCGTTTTAAAAACCTTGAAAGGAAGTCCGAAAGGGAAAGCTCAAAAAGGATAATATCTACTAGCGGTGAGCTTGAACGGTTAGAATTTGGTACTTGAGAGGCTATTTGGGATTTGTTTTCACAATTTTCTGTTCCAGATTCAGAGAAAATATGTTTGGATAACCAAATTCTAAAAGTTCTAAATTTTCTGATGAATCACTACGTCGTTGATGTGAGCATTTATCAATAAAACTGTGTTTCAGGTTGAAGAAGATTTGAAAAAAGGTGTCGCTGAAGCTGTGCCCGTCCCTCCTGATAATGCTGGGAAAGAGGAAGTCATTGCAGCATTGGTTGCTAATGTTGAAGCAATGATAAAAGCTGACCGCAAGATAGCTGCCTTGAAACAATTGCAAGTATGCAAGTAAAATGATTCTCAATTTATCTTACTACAATTCATTTGGCAGATTTTCTGAGTGAGCTGACTTCTAGCTATGCAGGGTCACATATGGAGAACAGGATTTTCAGGGAATGAATTGGAGGGAATAGTTTTTGATGATGTGCCAGAGGCTCTTGAGAGATGGCATGCTTTGGGCATTAAGGTTGTTCCCTTCCACCTCTTGATTCTTGCTAAAATCAGTACAAGTTTCTATTTGAGATTAAGTAGGAAAAGGTCTTCTCAATGTTCTAACTCCCGCGCTGGACCAGATGTAGACTGAACTATCTTGGGATGTTCAAAATCCAACTCGCCTCTTGCTTTAAAGGAAGAATGATCACGTACAGTTTCCTGAGAAGGGAGTGGCTACCTATTGGAGCTTCGACTGACTACCATCAGTCAATTCGACTTTGGGGCCACAACCTTTTTCTAACCCAAGTGACGAAGAAATGATGTGATTACTACTAAAATAAAAACGATATTTATTGATCTCTCTTAATTCAACAATCTAAGATTTGAATGGATGTCCTTTGAGTCCTCCAAAATTGCATTGATGCGTGGAGAAAGACTAAACTTAAGCATACTTGAGTCACTTTGAAGAAAAATTTTTGCAGATGTGTTCGAAGTGTTCCTCACAACATATTTAGAAAAGTGTAACGGCCCAAGCCTACCGCTAGCAAATATTGTCCTCTTTGAGCTTTCCCTTTTGAGCTTCCCCTCAAAATTTTTAAAACGCGTCTATTAGGAAGAGATTTCCACATTCTTATAAAGAATGTTTTATTCTCCTCTCCAACCGATGTGAGCTATGACAGAAAGATTAAACTCAGTTACTAAACTCAGTTATTAGATGAGACATATGATTTCATATTTGAGTTAGTAGTGACAGACCTGTCTAATAATAGATGTCGTGTGATTGATTTCCCTTCATTCTCATTTGGAATCCTGGTGATAATTGACCAAAAGTTCTCTGAGTCTCCGACAGACATTACGAACCAGTAATAACTTATATCGTTTCTGGCATGAACAGGTCTACATATACTCGAGCGGAAGCAGGTTGGCACAAAGGCTTATATTCGGAAAGACTAATTATGGTGATCTAAGAAAATACTTATCAGGATTCTTTGACACTGCTGTGGGGTAACCAATTTCTGTTCTTTTCATGTTATAATCATACTCGATATCACGAACATGATGGTGGAACTGCTCTTGTATCTCAGGAACAAAAGAGAAATAAGGAGTTATGTTGAGATTTCAGAGTCTCTTGGAGTTGATACACCATCAGAGATCCTGTTCATCACAGATGTCTATCAGGAAGCTGTAGCAGCAAAAGCAGCAGGTAGAGTCTCTCTACTTTTAGCTATTCTTTGCTGTTAGTTTTGGACGAAAAATTAGTAGAACCCGTTCGTGCTATATCGGAACTGCATTTGGCCAAAACGAAGTTCTTGTGACAAACTATCATATGGAGCTTCAAAAGAATTGCAGATTTACTTGTTTCATACTTGTTCAAATGCAATCCACGACGTTTCAGGGCTGCAGGTGGCCATCTCAATCCGACCAGGAAATGGTCCACTGCCTGATGATCATGGGTTCCAGACTGCCACTTCCTTCTCAGACATCACAGCTTGAGAGACTGTTCCAAGTAAGTTTAGCCTATTTTCTCCCATCTCTGCTAGTGATTGCTTAAAGTTTAAACTGAAGAAATATATATATATATAGCTGTACTTAATCTTGTCCTGGTCAAGCCTATGCAATAAAACTGAGTTCCAAATGAGTTTTTTGCTTCTGAA

mRNA sequence

AGGGCAGTGAAGGTATATCCGTTCTCTATCCTTCTTCTCTGATCTCTGTTGAATCACTCAAATGGCGATGGCTTCACCTCTGGCCGTCAATGGAGTCAAGTTGGCTACGACTTCTCTAGCTTATGTGGACACCAAGGCTGCAAACAATACGAAGGCTCTGATGGCGGAGCTTTCCCGCCAATTCTACGGCCTCCGATTGGTTTCCAGCCCCGCCGGTAGCATTGCCATCAAGGTTCACGACCACTCCATTCCCAAGGGACAACACACAATCTCTTTGTCACATTCCGGTGTTAAGAAGGAGAGAATGCTCGCGGAGGACACCTGCATGTTATCTTATGGTGGGTCAATCTTATATTCACCACCTCCAAAGCCATTTTCTCACAAGCTCCCGAACTGTTTGGATGGTAGACCTCTTTTTATGAAGGATCCTTTCGGGAGCATCAAAGGAATTTCAAGCAAAATTTCTGTGAAGGCTGGGGCTCAAGGTTTAAATAATGAAAACAAACCATTGCCACGTTGCATAGTTCTTGATATCGAAGGGACTACTACTCCAATATCTTTTGTTACTGACATACTCTTTCCATATGCTCGTGATAACGTTGGAAAGCATTTGATTTTAACGTATGAAACTGCAGAAACCCAAGATGACATTATGTTATTGCGTTCACAAGTTGAAGAAGATTTGAAAAAAGGTGTCGCTGAAGCTGTGCCCGTCCCTCCTGATAATGCTGGGAAAGAGGAAGTCATTGCAGCATTGGTTGCTAATGTTGAAGCAATGATAAAAGCTGACCGCAAGATAGCTGCCTTGAAACAATTGCAAGGTCACATATGGAGAACAGGATTTTCAGGGAATGAATTGGAGGGAATAGTTTTTGATGATGTGCCAGAGGCTCTTGAGAGATGGCATGCTTTGGGCATTAAGGTCTACATATACTCGAGCGGAAGCAGGTTGGCACAAAGGCTTATATTCGGAAAGACTAATTATGGTGATCTAAGAAAATACTTATCAGGATTCTTTGACACTGCTGTGGGGAACAAAAGAGAAATAAGGAGTTATGTTGAGATTTCAGAGTCTCTTGGAGTTGATACACCATCAGAGATCCTGTTCATCACAGATGTCTATCAGGAAGCTGTAGCAGCAAAAGCAGCAGGGCTGCAGGTGGCCATCTCAATCCGACCAGGAAATGGTCCACTGCCTGATGATCATGGGTTCCAGACTGCCACTTCCTTCTCAGACATCACAGCTTGAGAGACTGTTCCAAGTAAGTTTAGCCTATTTTCTCCCATCTCTGCTAGTGATTGCTTAAAGTTTAAACTGAAGAAATATATATATATATAGCTGTACTTAATCTTGTCCTGGTCAAGCCTATGCAATAAAACTGAGTTCCAAATGAGTTTTTTGCTTCTGAA

Coding sequence (CDS)

ATGGCGATGGCTTCACCTCTGGCCGTCAATGGAGTCAAGTTGGCTACGACTTCTCTAGCTTATGTGGACACCAAGGCTGCAAACAATACGAAGGCTCTGATGGCGGAGCTTTCCCGCCAATTCTACGGCCTCCGATTGGTTTCCAGCCCCGCCGGTAGCATTGCCATCAAGGTTCACGACCACTCCATTCCCAAGGGACAACACACAATCTCTTTGTCACATTCCGGTGTTAAGAAGGAGAGAATGCTCGCGGAGGACACCTGCATGTTATCTTATGGTGGGTCAATCTTATATTCACCACCTCCAAAGCCATTTTCTCACAAGCTCCCGAACTGTTTGGATGGTAGACCTCTTTTTATGAAGGATCCTTTCGGGAGCATCAAAGGAATTTCAAGCAAAATTTCTGTGAAGGCTGGGGCTCAAGGTTTAAATAATGAAAACAAACCATTGCCACGTTGCATAGTTCTTGATATCGAAGGGACTACTACTCCAATATCTTTTGTTACTGACATACTCTTTCCATATGCTCGTGATAACGTTGGAAAGCATTTGATTTTAACGTATGAAACTGCAGAAACCCAAGATGACATTATGTTATTGCGTTCACAAGTTGAAGAAGATTTGAAAAAAGGTGTCGCTGAAGCTGTGCCCGTCCCTCCTGATAATGCTGGGAAAGAGGAAGTCATTGCAGCATTGGTTGCTAATGTTGAAGCAATGATAAAAGCTGACCGCAAGATAGCTGCCTTGAAACAATTGCAAGGTCACATATGGAGAACAGGATTTTCAGGGAATGAATTGGAGGGAATAGTTTTTGATGATGTGCCAGAGGCTCTTGAGAGATGGCATGCTTTGGGCATTAAGGTCTACATATACTCGAGCGGAAGCAGGTTGGCACAAAGGCTTATATTCGGAAAGACTAATTATGGTGATCTAAGAAAATACTTATCAGGATTCTTTGACACTGCTGTGGGGAACAAAAGAGAAATAAGGAGTTATGTTGAGATTTCAGAGTCTCTTGGAGTTGATACACCATCAGAGATCCTGTTCATCACAGATGTCTATCAGGAAGCTGTAGCAGCAAAAGCAGCAGGGCTGCAGGTGGCCATCTCAATCCGACCAGGAAATGGTCCACTGCCTGATGATCATGGGTTCCAGACTGCCACTTCCTTCTCAGACATCACAGCTTGA

Protein sequence

MAMASPLAVNGVKLATTSLAYVDTKAANNTKALMAELSRQFYGLRLVSSPAGSIAIKVHDHSIPKGQHTISLSHSGVKKERMLAEDTCMLSYGGSILYSPPPKPFSHKLPNCLDGRPLFMKDPFGSIKGISSKISVKAGAQGLNNENKPLPRCIVLDIEGTTTPISFVTDILFPYARDNVGKHLILTYETAETQDDIMLLRSQVEEDLKKGVAEAVPVPPDNAGKEEVIAALVANVEAMIKADRKIAALKQLQGHIWRTGFSGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA
BLAST of CmaCh16G003990 vs. Swiss-Prot
Match: MTBC1_VITVI (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 OS=Vitis vinifera GN=VIT_19s0014g02480 PE=3 SV=2)

HSP 1 Score: 428.7 bits (1101), Expect = 6.9e-119
Identity = 212/271 (78.23%), Postives = 239/271 (88.19%), Query Frame = 1

Query: 125 GSIK--GISSKISVKAGAQGLNNENKPLPRCIVLDIEGTTTPISFVTDILFPYARDNVGK 184
           G IK  G  + +SVKAG    N+E +P  RCIVLDIEGTTTPISFVTD+LFP+AR+NV +
Sbjct: 247 GPIKKYGGKTNMSVKAGTVNSNHETEPSRRCIVLDIEGTTTPISFVTDVLFPFARNNVSR 306

Query: 185 HLILTYETAETQDDIMLLRSQVEEDLKKGVAEAVPVPPDNAGKEEVIAALVANVEAMIKA 244
           HL  TYET ETQDDI LLR+QV+ DL++G+  AVP+PPD+AGKEEVIAALVANVEAMIKA
Sbjct: 307 HLAATYETDETQDDIKLLRTQVQSDLEQGIVGAVPIPPDDAGKEEVIAALVANVEAMIKA 366

Query: 245 DRKIAALKQLQGHIWRTGFSGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQRLI 304
           DRKI ALKQLQGHIWRTGF  NELEG+VF+DVPEAL++WHA GIKVYIYSSGSRLAQRL+
Sbjct: 367 DRKITALKQLQGHIWRTGFQNNELEGVVFEDVPEALQKWHASGIKVYIYSSGSRLAQRLL 426

Query: 305 FGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAAKA 364
           FG TNYGDLRKYL GFFDT VGNKRE +SYVEI+ES+GVD PSEILF+TDVYQEAVAAKA
Sbjct: 427 FGYTNYGDLRKYLCGFFDTTVGNKRETKSYVEITESVGVDKPSEILFVTDVYQEAVAAKA 486

Query: 365 AGLQVAISIRPGNGPLPDDHGFQTATSFSDI 394
           AGL+V ISIRPGNGPLPD+HGF+T TSFSDI
Sbjct: 487 AGLEVIISIRPGNGPLPDNHGFKTITSFSDI 517

BLAST of CmaCh16G003990 vs. Swiss-Prot
Match: MTBC_POPTR (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Populus trichocarpa GN=POPTR_0001s40980g PE=3 SV=2)

HSP 1 Score: 427.6 bits (1098), Expect = 1.5e-118
Identity = 207/265 (78.11%), Postives = 234/265 (88.30%), Query Frame = 1

Query: 129 GISSKISVKAGAQGLNNENKPLPRCIVLDIEGTTTPISFVTDILFPYARDNVGKHLILTY 188
           G    + VKAG    NN  +PLPRCIVLDIEGTTTPI+FV D+LFPYARDNVG+HL  TY
Sbjct: 254 GPIQNVKVKAGMNNSNNRIEPLPRCIVLDIEGTTTPITFVADVLFPYARDNVGRHLSATY 313

Query: 189 ETAETQDDIMLLRSQVEEDLKKGVAEAVPVPPDNAGKEEVIAALVANVEAMIKADRKIAA 248
           +TAET+DDI LLR+QVE+DL +GV  A+P+P D+AGKEEVIAALVANVEAMIKADRKI A
Sbjct: 314 DTAETKDDINLLRTQVEDDLAQGVDGAIPIPTDDAGKEEVIAALVANVEAMIKADRKITA 373

Query: 249 LKQLQGHIWRTGFSGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKTNY 308
           LKQLQGHIWRTG+  NELEG+V+DDVPEALE+WHALGIKVYIYSSGSRLAQRLIFGKTNY
Sbjct: 374 LKQLQGHIWRTGYENNELEGVVYDDVPEALEKWHALGIKVYIYSSGSRLAQRLIFGKTNY 433

Query: 309 GDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAAKAAGLQVA 368
           GDLRKYLSGFFDT VGNK+E RSY+EISESLGVD PS+ILF+TDV+QEA AAK AGL V 
Sbjct: 434 GDLRKYLSGFFDTTVGNKKETRSYIEISESLGVDKPSDILFVTDVFQEAFAAKGAGLDVM 493

Query: 369 ISIRPGNGPLPDDHGFQTATSFSDI 394
           ISIRPGN PLP++HGF+T TSF++I
Sbjct: 494 ISIRPGNAPLPENHGFKTITSFAEI 518

BLAST of CmaCh16G003990 vs. Swiss-Prot
Match: MTBC_RICCO (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Ricinus communis GN=RCOM_0591060 PE=3 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 2.9e-117
Identity = 212/274 (77.37%), Postives = 238/274 (86.86%), Query Frame = 1

Query: 120 MKDPFGSIKGISSKISVKAGAQGLNNENKPLPRCIVLDIEGTTTPISFVTDILFPYARDN 179
           +K   GS + I++  SVKAG +  N+  + LP CIVLDIEGTTTPI+FV D+LFPYARDN
Sbjct: 256 VKALIGSNRDINT--SVKAGLKDSNHGMQSLPGCIVLDIEGTTTPITFVADVLFPYARDN 315

Query: 180 VGKHLILTYETAETQDDIMLLRSQVEEDLKKGVAEAVPVPPDNAGKEEVIAALVANVEAM 239
           VG+HL  TYETAETQDDI LLR+QVE+DL +GV EAV +PPD+AGKEEVIAALVANVEAM
Sbjct: 316 VGRHLYATYETAETQDDIKLLRTQVEDDLARGVNEAVSIPPDDAGKEEVIAALVANVEAM 375

Query: 240 IKADRKIAALKQLQGHIWRTGFSGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQ 299
           IKADRKI ALKQLQGHIWRTGF  NELEG+V+DDVPEALE+WHALGIKVYIYSSGSRLAQ
Sbjct: 376 IKADRKITALKQLQGHIWRTGFQNNELEGVVYDDVPEALEKWHALGIKVYIYSSGSRLAQ 435

Query: 300 RLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVA 359
           RLIFGKTNYGDLRKYLSGFFDT VGNKRE RSY+EISES+GVD PSEILF+TDV QEAVA
Sbjct: 436 RLIFGKTNYGDLRKYLSGFFDTTVGNKRETRSYIEISESVGVDRPSEILFVTDVVQEAVA 495

Query: 360 AKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDI 394
           AK AGL+  ISIR GN PLP++HGF+T  S S+I
Sbjct: 496 AKGAGLEAVISIRQGNAPLPENHGFKTINSLSEI 527

BLAST of CmaCh16G003990 vs. Swiss-Prot
Match: MTBC_ORYSJ (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Oryza sativa subsp. japonica GN=Os11g0484000 PE=2 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 1.1e-103
Identity = 182/248 (73.39%), Postives = 213/248 (85.89%), Query Frame = 1

Query: 146 ENKPLPRCIVLDIEGTTTPISFVTDILFPYARDNVGKHLILTYETAETQDDIMLLRSQVE 205
           ++K    C+VLDIEGTTTPISFVTD++FPYARDNV KHL  TY + ET++DI LLR QVE
Sbjct: 271 DSKSSKHCVVLDIEGTTTPISFVTDVMFPYARDNVRKHLTSTYSSDETKEDIKLLRIQVE 330

Query: 206 EDLKKGVAEAVPVPPDNAGKEEVIAALVANVEAMIKADRKIAALKQLQGHIWRTGFSGNE 265
           EDLK G+  +VP+PPD+A KEEVI ALVANVE+MIKADRKI +LKQLQGHIWRTGF   E
Sbjct: 331 EDLKNGIVGSVPIPPDDADKEEVINALVANVESMIKADRKITSLKQLQGHIWRTGFESKE 390

Query: 266 LEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGN 325
           L+G+VFDDVPEAL+ WHA G+KVYIYSSGSR AQRL+FG T YGDLR+YL GFFDT  GN
Sbjct: 391 LQGVVFDDVPEALKHWHASGMKVYIYSSGSREAQRLLFGNTAYGDLRQYLCGFFDTTTGN 450

Query: 326 KREIRSYVEISESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQ 385
           KRE RSY EIS+SLGVD+P++ILFITDV+QEAVAAK+AG +V ISIRPGN PLP++HGF+
Sbjct: 451 KRETRSYFEISQSLGVDSPAQILFITDVFQEAVAAKSAGFEVIISIRPGNAPLPENHGFR 510

Query: 386 TATSFSDI 394
           T  SFS+I
Sbjct: 511 TIKSFSEI 518

BLAST of CmaCh16G003990 vs. Swiss-Prot
Match: MTBC_ORYSI (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Oryza sativa subsp. indica GN=OsI_36120 PE=2 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 1.1e-103
Identity = 182/248 (73.39%), Postives = 213/248 (85.89%), Query Frame = 1

Query: 146 ENKPLPRCIVLDIEGTTTPISFVTDILFPYARDNVGKHLILTYETAETQDDIMLLRSQVE 205
           ++K    C+VLDIEGTTTPISFVTD++FPYARDNV KHL  TY + ET++DI LLR QVE
Sbjct: 271 DSKSSKHCVVLDIEGTTTPISFVTDVMFPYARDNVRKHLTSTYSSDETKEDIKLLRIQVE 330

Query: 206 EDLKKGVAEAVPVPPDNAGKEEVIAALVANVEAMIKADRKIAALKQLQGHIWRTGFSGNE 265
           EDLK G+  +VP+PPD+A KEEVI ALVANVE+MIKADRKI +LKQLQGHIWRTGF   E
Sbjct: 331 EDLKNGIVGSVPIPPDDADKEEVINALVANVESMIKADRKITSLKQLQGHIWRTGFESKE 390

Query: 266 LEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGN 325
           L+G+VFDDVPEAL+ WHA G+KVYIYSSGSR AQRL+FG T YGDLR+YL GFFDT  GN
Sbjct: 391 LQGVVFDDVPEALKHWHASGMKVYIYSSGSREAQRLLFGNTAYGDLRQYLCGFFDTTTGN 450

Query: 326 KREIRSYVEISESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQ 385
           KRE RSY EIS+SLGVD+P++ILFITDV+QEAVAAK+AG +V ISIRPGN PLP++HGF+
Sbjct: 451 KRETRSYFEISQSLGVDSPAQILFITDVFQEAVAAKSAGFEVIISIRPGNAPLPENHGFR 510

Query: 386 TATSFSDI 394
           T  SFS+I
Sbjct: 511 TIKSFSEI 518

BLAST of CmaCh16G003990 vs. TrEMBL
Match: A0A0A0LAF4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G233480 PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 1.5e-176
Identity = 327/418 (78.23%), Postives = 352/418 (84.21%), Query Frame = 1

Query: 1   MAMASPLAVNGVKLATTSLAYVDTKAANNTKALMAELSRQFYGLRLVSSPAGSIAIKVHD 60
           MAMAS +A+N +KLA+TS A ++ KAAN+TKAL+++LSRQFY LR VS    SIAIKVHD
Sbjct: 1   MAMASAMALNPLKLASTSQACLEKKAANDTKALLSDLSRQFYTLRCVSGTGSSIAIKVHD 60

Query: 61  HSIPKGQHTISLSHSGVKKERMLAEDTCMLSYGGSILYSPPPKPFSHKLPNCLDGRPLFM 120
           HSIP+ Q  I+ S SGVKK RM+ EDT MLSYGGSILYSPPPKPFSHKLPNC D RPLFM
Sbjct: 61  HSIPRRQQLIATSLSGVKKRRMVREDTYMLSYGGSILYSPPPKPFSHKLPNCFDSRPLFM 120

Query: 121 K-------------------------DPFGSIKGISSKISVKAGAQGLNNENKPLPRCIV 180
           K                            GSIKGISS ISVKAGAQGL+N+N+ LPRCIV
Sbjct: 121 KMRNAGGVIHRHSKESYLVTLRNHHQGRIGSIKGISSNISVKAGAQGLDNKNEWLPRCIV 180

Query: 181 LDIEGTTTPISFVTDILFPYARDNVGKHLILTYETAETQDDIMLLRSQVEEDLKKGVAEA 240
           LDIEGTTTPISFVTD+LFPYARDNV KHLILTYET ETQDDI LLRSQVEEDL+KGVA A
Sbjct: 181 LDIEGTTTPISFVTDVLFPYARDNVEKHLILTYETGETQDDIKLLRSQVEEDLEKGVAGA 240

Query: 241 VPVPPDNAGKEEVIAALVANVEAMIKADRKIAALKQLQGHIWRTGFSGNELEGIVFDDVP 300
           VP+PPDNAGKEEVIAA+V NVE MIKADRKI ALKQLQGHIWRTGFS NELEG+VFDDVP
Sbjct: 241 VPIPPDNAGKEEVIAAVVGNVEGMIKADRKITALKQLQGHIWRTGFSVNELEGVVFDDVP 300

Query: 301 EALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEI 360
           EALERWHA GIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKRE  SYVEI
Sbjct: 301 EALERWHASGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKRETSSYVEI 360

Query: 361 SESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDI 394
           SES+GVD+PSEILFITDV QEA AAKAAGLQVAISIRPGNGPL D+HGFQT TSFS+I
Sbjct: 361 SESVGVDSPSEILFITDVCQEAKAAKAAGLQVAISIRPGNGPLSDNHGFQTITSFSEI 418

BLAST of CmaCh16G003990 vs. TrEMBL
Match: A0A059BMC0_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F01149 PE=4 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 1.3e-129
Identity = 245/395 (62.03%), Postives = 295/395 (74.68%), Query Frame = 1

Query: 1   MAMASPLAVNGVKLATTSLAYVDTKAANNTKALMAELSRQFYGLRLVSSPAGSIAIKVHD 60
           MA +  +A+NG      S  Y++ KA    +   ++L R+F  L   SS  G IA++V  
Sbjct: 45  MASSPVVALNGALAGRASRGYLERKAPAKARIQASKLCRRFCTLGWASSAGGGIAVEVRR 104

Query: 61  HSIPKGQHTISLSHS-GVKKERMLAEDTCMLSYGGSILYSPPPKPFSHKLPNCLDGRPLF 120
            S+P+    I+     G +K+R++ +    LS  GSIL  P PK +S K P C+      
Sbjct: 105 DSVPRSNRLIAAGRRPGARKDRVMPDYMYGLSANGSILPLPSPKLYSSKPPRCMSCLSFC 164

Query: 121 MKDPF-GSIKGISSKISVKAGAQGLNNENKPLPRCIVLDIEGTTTPISFVTDILFPYARD 180
            K    G+    + +  VKAGA   +N  +PLPRCIVLDIEGTTTPISFVTD+LFPYARD
Sbjct: 165 TKQTGKGAASYANIRTYVKAGALNSDNRTEPLPRCIVLDIEGTTTPISFVTDVLFPYARD 224

Query: 181 NVGKHLILTYETAETQDDIMLLRSQVEEDLKKGVAEAVPVPPDNAGKEEVIAALVANVEA 240
           NVG+HL  T+ET ET+DDI LLRSQVE+DLK+GV  AVP+P D AGKE+VIAALVANVEA
Sbjct: 225 NVGRHLSATFETPETRDDIKLLRSQVEDDLKQGVQGAVPIPGDEAGKEQVIAALVANVEA 284

Query: 241 MIKADRKIAALKQLQGHIWRTGFSGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLA 300
           MIKADRKI ALKQLQGHIW+TGF  NELEG+VF+DVP+AL  WH+LGIKVYIYSSGSRLA
Sbjct: 285 MIKADRKITALKQLQGHIWQTGFENNELEGVVFEDVPKALTNWHSLGIKVYIYSSGSRLA 344

Query: 301 QRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAV 360
           QRLIFGKTNYGDLRKYL GFFDT VGNK+E RSY+EISESLGVD PS++LF+TDVYQEAV
Sbjct: 345 QRLIFGKTNYGDLRKYLCGFFDTYVGNKKETRSYIEISESLGVDKPSDVLFLTDVYQEAV 404

Query: 361 AAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDI 394
           AAK AGL+V I IRPGNGPLP++HGF+T +SF +I
Sbjct: 405 AAKDAGLKVLILIRPGNGPLPENHGFKTVSSFLEI 439

BLAST of CmaCh16G003990 vs. TrEMBL
Match: A0A059BMP2_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F01149 PE=4 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 6.2e-127
Identity = 246/424 (58.02%), Postives = 297/424 (70.05%), Query Frame = 1

Query: 1   MAMASPLAVNGVKLATTSLAYVDTKAANNTKALMAELSRQFYGLRLVSSPAGSIAIKVHD 60
           MA +  +A+NG      S  Y++ KA    +   ++L R+F  L   SS  G IA++V  
Sbjct: 45  MASSPVVALNGALAGRASRGYLERKAPAKARIQASKLCRRFCTLGWASSAGGGIAVEVRR 104

Query: 61  HSIPKGQHTISLSHS-GVKKERMLAEDTCMLSYGGSILYSPPPKPFSHKLPNCL------ 120
            S+P+    I+     G +K+R++ +    LS  GSIL  P PK +S K P C+      
Sbjct: 105 DSVPRSNRLIAAGRRPGARKDRVMPDYMYGLSANGSILPLPSPKLYSSKPPRCMSCLSFC 164

Query: 121 ---DGRPLFMKDPF---------------------GSIKGISSKISVKAGAQGLNNENKP 180
              +G      D                       G+    + +  VKAGA   +N  +P
Sbjct: 165 TKAEGYHFLFNDALELHHLGLNNLIPNHGLQQTGKGAASYANIRTYVKAGALNSDNRTEP 224

Query: 181 LPRCIVLDIEGTTTPISFVTDILFPYARDNVGKHLILTYETAETQDDIMLLRSQVEEDLK 240
           LPRCIVLDIEGTTTPISFVTD+LFPYARDNVG+HL  T+ET ET+DDI LLRSQVE+DLK
Sbjct: 225 LPRCIVLDIEGTTTPISFVTDVLFPYARDNVGRHLSATFETPETRDDIKLLRSQVEDDLK 284

Query: 241 KGVAEAVPVPPDNAGKEEVIAALVANVEAMIKADRKIAALKQLQGHIWRTGFSGNELEGI 300
           +GV  AVP+P D AGKE+VIAALVANVEAMIKADRKI ALKQLQGHIW+TGF  NELEG+
Sbjct: 285 QGVQGAVPIPGDEAGKEQVIAALVANVEAMIKADRKITALKQLQGHIWQTGFENNELEGV 344

Query: 301 VFDDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREI 360
           VF+DVP+AL  WH+LGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYL GFFDT VGNK+E 
Sbjct: 345 VFEDVPKALTNWHSLGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLCGFFDTYVGNKKET 404

Query: 361 RSYVEISESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATS 394
           RSY+EISESLGVD PS++LF+TDVYQEAVAAK AGL+V I IRPGNGPLP++HGF+T +S
Sbjct: 405 RSYIEISESLGVDKPSDVLFLTDVYQEAVAAKDAGLKVLILIRPGNGPLPENHGFKTVSS 464

BLAST of CmaCh16G003990 vs. TrEMBL
Match: A0A059BNB1_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F01149 PE=4 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 1.9e-120
Identity = 233/393 (59.29%), Postives = 278/393 (70.74%), Query Frame = 1

Query: 1   MAMASPLAVNGVKLATTSLAYVDTKAANNTKALMAELSRQFYGLRLVSSPAGSIAIKVHD 60
           MA +  +A+NG      S  Y++ KA    +   ++L R+F  L   SS  G IA++V  
Sbjct: 45  MASSPVVALNGALAGRASRGYLERKAPAKARIQASKLCRRFCTLGWASSAGGGIAVEVRR 104

Query: 61  HSIPKGQHTISLSHSGVKKERMLAEDTCMLSYGGSILYSPPPKPFSHKLPNCLDGRPLFM 120
            S+P+    I+                C+    G            H   N L       
Sbjct: 105 DSVPRSNRLIAAGR----------RPACIFQAEGYHFLFNDALELHHLGLNNLIPNHGLQ 164

Query: 121 KDPFGSIKGISSKISVKAGAQGLNNENKPLPRCIVLDIEGTTTPISFVTDILFPYARDNV 180
           +   G+    + +  VKAGA   +N  +PLPRCIVLDIEGTTTPISFVTD+LFPYARDNV
Sbjct: 165 QTGKGAASYANIRTYVKAGALNSDNRTEPLPRCIVLDIEGTTTPISFVTDVLFPYARDNV 224

Query: 181 GKHLILTYETAETQDDIMLLRSQVEEDLKKGVAEAVPVPPDNAGKEEVIAALVANVEAMI 240
           G+HL  T+ET ET+DDI LLRSQVE+DLK+GV  AVP+P D AGKE+VIAALVANVEAMI
Sbjct: 225 GRHLSATFETPETRDDIKLLRSQVEDDLKQGVQGAVPIPGDEAGKEQVIAALVANVEAMI 284

Query: 241 KADRKIAALKQLQGHIWRTGFSGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR 300
           KADRKI ALKQLQGHIW+TGF  NELEG+VF+DVP+AL  WH+LGIKVYIYSSGSRLAQR
Sbjct: 285 KADRKITALKQLQGHIWQTGFENNELEGVVFEDVPKALTNWHSLGIKVYIYSSGSRLAQR 344

Query: 301 LIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAA 360
           LIFGKTNYGDLRKYL GFFDT VGNK+E RSY+EISESLGVD PS++LF+TDVYQEAVAA
Sbjct: 345 LIFGKTNYGDLRKYLCGFFDTYVGNKKETRSYIEISESLGVDKPSDVLFLTDVYQEAVAA 404

Query: 361 KAAGLQVAISIRPGNGPLPDDHGFQTATSFSDI 394
           K AGL+V I IRPGNGPLP++HGF+T +SF +I
Sbjct: 405 KDAGLKVLILIRPGNGPLPENHGFKTVSSFLEI 427

BLAST of CmaCh16G003990 vs. TrEMBL
Match: A0A0D2SFC4_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_013G170700 PE=4 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 2.0e-117
Identity = 212/280 (75.71%), Postives = 241/280 (86.07%), Query Frame = 1

Query: 114 DGRPLFMKDPFGSIKGISSKISVKAGAQGLNNENKPLPRCIVLDIEGTTTPISFVTDILF 173
           D  P+     F SI  +  +++ KAG    N + +P PRC+VLDIEGTTTPISFVTD+LF
Sbjct: 85  DHGPIQNVKGFSSINSVIKRVATKAGTVDSNFKLQPYPRCVVLDIEGTTTPISFVTDVLF 144

Query: 174 PYARDNVGKHLILTYETAETQDDIMLLRSQVEEDLKKGVAEAVPVPPDNAGKEEVIAALV 233
           PYARDNVG+HL  TY++AETQDDI LLRSQVE+DLK+GV  AVP+P ++AGKE+VIA+LV
Sbjct: 145 PYARDNVGRHLSATYDSAETQDDIKLLRSQVEDDLKQGVLGAVPIPSEDAGKEKVIASLV 204

Query: 234 ANVEAMIKADRKIAALKQLQGHIWRTGFSGNELEGIVFDDVPEALERWHALGIKVYIYSS 293
           ANVEAMIKADRKI ALKQLQGHIWRTGF  NELEG+VFDDVPEALE+WHALGIKVYIYSS
Sbjct: 205 ANVEAMIKADRKITALKQLQGHIWRTGFENNELEGVVFDDVPEALEKWHALGIKVYIYSS 264

Query: 294 GSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDV 353
           GSRLAQRLIFG TNYGDLRKYLSGFFDTAVG KRE RSYVEI+ESLGVD PS+ILF+TDV
Sbjct: 265 GSRLAQRLIFGNTNYGDLRKYLSGFFDTAVGYKRETRSYVEITESLGVDKPSDILFVTDV 324

Query: 354 YQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDI 394
           YQEA AAKAAGL+V ISIR GNGPLP++HGF+T  SF +I
Sbjct: 325 YQEATAAKAAGLEVVISIRSGNGPLPENHGFKTINSFLEI 364

BLAST of CmaCh16G003990 vs. TAIR10
Match: AT5G53850.2 (AT5G53850.2 haloacid dehalogenase-like hydrolase family protein)

HSP 1 Score: 377.9 bits (969), Expect = 7.8e-105
Identity = 182/242 (75.21%), Postives = 216/242 (89.26%), Query Frame = 1

Query: 152 RCIVLDIEGTTTPISFVTDILFPYARDNVGKHLILTYETAETQDDIMLLRSQVEEDLKKG 211
           R IVLDIEGTTTPI+FVTD+LFPYAR+NVGKHL LTY TAETQ+DI LLR+QVEEDL++G
Sbjct: 266 RWIVLDIEGTTTPITFVTDVLFPYARENVGKHLNLTYHTAETQEDIKLLRAQVEEDLREG 325

Query: 212 VAEAVPVPPDNAGKEEVIAALVANVEAMIKADRKIAALKQLQGHIWRTGFSGNELEGIVF 271
           V  AVP+P  + GKE+VIAA+V+NVEAMI+ADRKI ALK+LQGHIWRTGF  +EL+ IVF
Sbjct: 326 VTGAVPIPHADEGKEKVIAAMVSNVEAMIRADRKITALKELQGHIWRTGFECDELKAIVF 385

Query: 272 DDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRS 331
           +DV +ALE+WH+ GIKVYIYSSGSRLAQ+L+FG T+YGDLRKY+SGFFDT +GNK+E RS
Sbjct: 386 EDVADALEKWHSSGIKVYIYSSGSRLAQKLLFGNTDYGDLRKYISGFFDTTIGNKKESRS 445

Query: 332 YVEISESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFS 391
           Y EI E+LGVD P+EI+F+TDVYQEAVAAKAAGL+  ISIRPGN PLP++HGF+T TSFS
Sbjct: 446 YKEIKETLGVDDPAEIMFVTDVYQEAVAAKAAGLEAIISIRPGNAPLPENHGFKTVTSFS 505

Query: 392 DI 394
            I
Sbjct: 506 QI 507

BLAST of CmaCh16G003990 vs. NCBI nr
Match: gi|659112020|ref|XP_008456026.1| (PREDICTED: probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X2 [Cucumis melo])

HSP 1 Score: 647.5 bits (1669), Expect = 1.5e-182
Identity = 329/393 (83.72%), Postives = 355/393 (90.33%), Query Frame = 1

Query: 1   MAMASPLAVNGVKLATTSLAYVDTKAANNTKALMAELSRQFYGLRLVSSPAGSIAIKVHD 60
           MAMAS L ++GVKLATTS AY++TKAAN+TKAL++ELSRQFY LR VS    SIAIKVHD
Sbjct: 1   MAMASALPLSGVKLATTSQAYMETKAANDTKALLSELSRQFYTLRCVSGTGSSIAIKVHD 60

Query: 61  HSIPKGQHTISLSHSGVKKERMLAEDTCMLSYGGSILYSPPPKPFSHKLPNCLDGRPLFM 120
           HSIP+ Q  I++S SGVKK RM+ EDT MLS GGSIL+SPPPKPFSHKLPNC D RPLFM
Sbjct: 61  HSIPRRQQLIAMSLSGVKKRRMVREDTYMLSSGGSILHSPPPKPFSHKLPNCFDSRPLFM 120

Query: 121 KDPFGSIKGISSKISVKAGAQGLNNENKPLPRCIVLDIEGTTTPISFVTDILFPYARDNV 180
           K   GSIKGISS ISVKAGA+GL+N+N+  PRCIVLDIEGTTTPISFVTD+LF YARDNV
Sbjct: 121 KGQIGSIKGISSNISVKAGARGLDNKNQWFPRCIVLDIEGTTTPISFVTDVLFSYARDNV 180

Query: 181 GKHLILTYETAETQDDIMLLRSQVEEDLKKGVAEAVPVPPDNAGKEEVIAALVANVEAMI 240
            KHLILTYET ETQDDI LLRSQVEEDL+KGVA AVP+PPDNAGKEEVIAA+VANVE MI
Sbjct: 181 EKHLILTYETGETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAAVVANVEGMI 240

Query: 241 KADRKIAALKQLQGHIWRTGFSGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR 300
           KADRKI ALKQLQGHIWRTGFSGNELEG+VFDDVPEALERWHA GIK+YIYSSGSRLAQR
Sbjct: 241 KADRKITALKQLQGHIWRTGFSGNELEGVVFDDVPEALERWHASGIKMYIYSSGSRLAQR 300

Query: 301 LIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAA 360
           LIFGKTNYGDLRKYLSGFFDTAVGNKRE  SYVEISES+GVD+PSEILFITDV QEA AA
Sbjct: 301 LIFGKTNYGDLRKYLSGFFDTAVGNKRETSSYVEISESVGVDSPSEILFITDVCQEAKAA 360

Query: 361 KAAGLQVAISIRPGNGPLPDDHGFQTATSFSDI 394
           KAAGLQVAISIRPGNGPLPD+HGFQT TSFS+I
Sbjct: 361 KAAGLQVAISIRPGNGPLPDNHGFQTITSFSEI 393

BLAST of CmaCh16G003990 vs. NCBI nr
Match: gi|659112018|ref|XP_008456025.1| (PREDICTED: probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X1 [Cucumis melo])

HSP 1 Score: 633.6 bits (1633), Expect = 2.2e-178
Identity = 329/418 (78.71%), Postives = 355/418 (84.93%), Query Frame = 1

Query: 1   MAMASPLAVNGVKLATTSLAYVDTKAANNTKALMAELSRQFYGLRLVSSPAGSIAIKVHD 60
           MAMAS L ++GVKLATTS AY++TKAAN+TKAL++ELSRQFY LR VS    SIAIKVHD
Sbjct: 1   MAMASALPLSGVKLATTSQAYMETKAANDTKALLSELSRQFYTLRCVSGTGSSIAIKVHD 60

Query: 61  HSIPKGQHTISLSHSGVKKERMLAEDTCMLSYGGSILYSPPPKPFSHKLPNCLDGRPLFM 120
           HSIP+ Q  I++S SGVKK RM+ EDT MLS GGSIL+SPPPKPFSHKLPNC D RPLFM
Sbjct: 61  HSIPRRQQLIAMSLSGVKKRRMVREDTYMLSSGGSILHSPPPKPFSHKLPNCFDSRPLFM 120

Query: 121 K-------------------------DPFGSIKGISSKISVKAGAQGLNNENKPLPRCIV 180
           K                            GSIKGISS ISVKAGA+GL+N+N+  PRCIV
Sbjct: 121 KMRNAGAVIHSHSKESYLVTQRNHHQGQIGSIKGISSNISVKAGARGLDNKNQWFPRCIV 180

Query: 181 LDIEGTTTPISFVTDILFPYARDNVGKHLILTYETAETQDDIMLLRSQVEEDLKKGVAEA 240
           LDIEGTTTPISFVTD+LF YARDNV KHLILTYET ETQDDI LLRSQVEEDL+KGVA A
Sbjct: 181 LDIEGTTTPISFVTDVLFSYARDNVEKHLILTYETGETQDDIKLLRSQVEEDLEKGVAGA 240

Query: 241 VPVPPDNAGKEEVIAALVANVEAMIKADRKIAALKQLQGHIWRTGFSGNELEGIVFDDVP 300
           VP+PPDNAGKEEVIAA+VANVE MIKADRKI ALKQLQGHIWRTGFSGNELEG+VFDDVP
Sbjct: 241 VPIPPDNAGKEEVIAAVVANVEGMIKADRKITALKQLQGHIWRTGFSGNELEGVVFDDVP 300

Query: 301 EALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEI 360
           EALERWHA GIK+YIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKRE  SYVEI
Sbjct: 301 EALERWHASGIKMYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKRETSSYVEI 360

Query: 361 SESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDI 394
           SES+GVD+PSEILFITDV QEA AAKAAGLQVAISIRPGNGPLPD+HGFQT TSFS+I
Sbjct: 361 SESVGVDSPSEILFITDVCQEAKAAKAAGLQVAISIRPGNGPLPDNHGFQTITSFSEI 418

BLAST of CmaCh16G003990 vs. NCBI nr
Match: gi|778680342|ref|XP_011651293.1| (PREDICTED: probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 [Cucumis sativus])

HSP 1 Score: 627.1 bits (1616), Expect = 2.1e-176
Identity = 327/418 (78.23%), Postives = 352/418 (84.21%), Query Frame = 1

Query: 1   MAMASPLAVNGVKLATTSLAYVDTKAANNTKALMAELSRQFYGLRLVSSPAGSIAIKVHD 60
           MAMAS +A+N +KLA+TS A ++ KAAN+TKAL+++LSRQFY LR VS    SIAIKVHD
Sbjct: 1   MAMASAMALNPLKLASTSQACLEKKAANDTKALLSDLSRQFYTLRCVSGTGSSIAIKVHD 60

Query: 61  HSIPKGQHTISLSHSGVKKERMLAEDTCMLSYGGSILYSPPPKPFSHKLPNCLDGRPLFM 120
           HSIP+ Q  I+ S SGVKK RM+ EDT MLSYGGSILYSPPPKPFSHKLPNC D RPLFM
Sbjct: 61  HSIPRRQQLIATSLSGVKKRRMVREDTYMLSYGGSILYSPPPKPFSHKLPNCFDSRPLFM 120

Query: 121 K-------------------------DPFGSIKGISSKISVKAGAQGLNNENKPLPRCIV 180
           K                            GSIKGISS ISVKAGAQGL+N+N+ LPRCIV
Sbjct: 121 KMRNAGGVIHRHSKESYLVTLRNHHQGRIGSIKGISSNISVKAGAQGLDNKNEWLPRCIV 180

Query: 181 LDIEGTTTPISFVTDILFPYARDNVGKHLILTYETAETQDDIMLLRSQVEEDLKKGVAEA 240
           LDIEGTTTPISFVTD+LFPYARDNV KHLILTYET ETQDDI LLRSQVEEDL+KGVA A
Sbjct: 181 LDIEGTTTPISFVTDVLFPYARDNVEKHLILTYETGETQDDIKLLRSQVEEDLEKGVAGA 240

Query: 241 VPVPPDNAGKEEVIAALVANVEAMIKADRKIAALKQLQGHIWRTGFSGNELEGIVFDDVP 300
           VP+PPDNAGKEEVIAA+V NVE MIKADRKI ALKQLQGHIWRTGFS NELEG+VFDDVP
Sbjct: 241 VPIPPDNAGKEEVIAAVVGNVEGMIKADRKITALKQLQGHIWRTGFSVNELEGVVFDDVP 300

Query: 301 EALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEI 360
           EALERWHA GIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKRE  SYVEI
Sbjct: 301 EALERWHASGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKRETSSYVEI 360

Query: 361 SESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDI 394
           SES+GVD+PSEILFITDV QEA AAKAAGLQVAISIRPGNGPL D+HGFQT TSFS+I
Sbjct: 361 SESVGVDSPSEILFITDVCQEAKAAKAAGLQVAISIRPGNGPLSDNHGFQTITSFSEI 418

BLAST of CmaCh16G003990 vs. NCBI nr
Match: gi|747100405|ref|XP_011098291.1| (PREDICTED: probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X1 [Sesamum indicum])

HSP 1 Score: 496.9 bits (1278), Expect = 3.3e-137
Identity = 262/423 (61.94%), Postives = 307/423 (72.58%), Query Frame = 1

Query: 2   AMASPLAVNGVKLATTSLAYVDTKAANNTKALMAELSRQFYGLRLVSSPAGSIAIKVHDH 61
           A A+P  +NG   A  + AY+D      TKAL+ E  RQFY L   S   GSI IKVHD 
Sbjct: 4   ASAAPFTING---AVKTQAYLDGSRVKETKALIPEFCRQFYHLGWESGAGGSITIKVHDD 63

Query: 62  SIPKGQHTISLSHSGVKKERMLAEDTCMLSYGGSILYSPPPKPFSHKLPNCLDGRPLFMK 121
           S PK    I +S SGV+KE+M+ ED  +LS  G +L  P PK   +K P C D  PLF++
Sbjct: 64  STPKPHQPIVMSPSGVQKEKMMEEDMYVLSPDGFVLSEPLPKSCPNKPPKCSDYWPLFLE 123

Query: 122 --------------------------DPFGSIKGI-----SSKISVKAGAQGLNNENKPL 181
                                      P  S   +     ++K+S KAG +  NN  +P 
Sbjct: 124 AECYHYLSDAAIKLYQLGLNWSTPSHGPIRSPNAVLGSNRNAKVSAKAGTRSPNNGTEPS 183

Query: 182 PRCIVLDIEGTTTPISFVTDILFPYARDNVGKHLILTYETAETQDDIMLLRSQVEEDLKK 241
            RCIVLDIEGTTTPISFVTD+LFPYARD+VGKHL LTY+TAETQDDI LLR+QVEEDL+ 
Sbjct: 184 RRCIVLDIEGTTTPISFVTDVLFPYARDSVGKHLELTYDTAETQDDIKLLRAQVEEDLEN 243

Query: 242 GVAEAVPVPPDNAGKEEVIAALVANVEAMIKADRKIAALKQLQGHIWRTGFSGNELEGIV 301
           GV +AVP+P D   KE++I A+VANVEAMIKADRKI +LKQLQGHIWRTGF  NELE ++
Sbjct: 244 GVPDAVPIPTDGV-KEDIIPAVVANVEAMIKADRKITSLKQLQGHIWRTGFQSNELETVI 303

Query: 302 FDDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIR 361
           FDDVPEALE+WH+LGIKVYIYSSGSRLAQRL+FG TNYGDLRKYLSGFFDT VGNK+E +
Sbjct: 304 FDDVPEALEKWHSLGIKVYIYSSGSRLAQRLLFGNTNYGDLRKYLSGFFDTTVGNKKETK 363

Query: 362 SYVEISESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSF 394
           SY+EI+ESLGVD PSEILF+TDVYQEA AAKAAGL+V ISIRPGNGPLPD+HGF+T TSF
Sbjct: 364 SYIEITESLGVDNPSEILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPDNHGFRTITSF 422

BLAST of CmaCh16G003990 vs. NCBI nr
Match: gi|720031130|ref|XP_010265717.1| (PREDICTED: probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 [Nelumbo nucifera])

HSP 1 Score: 490.7 bits (1262), Expect = 2.3e-135
Identity = 259/406 (63.79%), Postives = 302/406 (74.38%), Query Frame = 1

Query: 1   MAMASPLAVNGVKLATTSLAYVDTKAANNTKALMAELSRQFYGLRLVSSPAGSIAIKVHD 60
           MA AS   VNGV              +  T+AL++EL RQFY L   S   GSIAIKVHD
Sbjct: 1   MAAASAAVVNGV--------------SQETRALVSELRRQFYTLGWTSGTGGSIAIKVHD 60

Query: 61  HSIPKGQHTISLS-HSGVKKERMLAEDTCMLSYGGSILYSPPPKPFSHKLPNCLDGRPLF 120
            SIPK Q  I +S  SGV+KERM+ ED  +LS  GS+L  P PKP+ HK P C +   LF
Sbjct: 61  DSIPKPQQLIVMSPSSGVQKERMVPEDMYVLSPSGSVLKVPFPKPYPHKPPKCSECASLF 120

Query: 121 MK----------DPFGSIKGI--SSKISVKAGAQGLNNENKPLPRCIVLDIEGTTTPISF 180
           +K           P+  + G   ++ I+VKAG   LN+  +P  RCIVLDIEGTTTPISF
Sbjct: 121 IKLGLNRSTPNYGPYHRVSGAERNTNIAVKAGTMNLNHSPEPSRRCIVLDIEGTTTPISF 180

Query: 181 VTDILFPYARDNVGKHLILTYETAETQDDIMLLRSQVEEDLKKGVAEAVPVPPDNAGKEE 240
           V D LFPYARDNVGKHL  TYET ETQ DI LLR+Q+++DLK+GV  AVP+P D+AGKEE
Sbjct: 181 VMDALFPYARDNVGKHLTATYETEETQYDIKLLRAQIKDDLKQGVIGAVPIPLDDAGKEE 240

Query: 241 VIAALVANVEAMIKADRKIAALKQLQGHIWRTGFSGNELEGIVFDDVPEALERWHALGIK 300
           +I +LV+NV AMIKADRK+ ALKQLQGHIWRTGF  NEL+G+VFDDVPEALE+WHA GIK
Sbjct: 241 IIRSLVSNVGAMIKADRKVTALKQLQGHIWRTGFQNNELKGVVFDDVPEALEKWHASGIK 300

Query: 301 VYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEI 360
           VYIYSSGSR AQRLIFG +NYGDLRKYL GFFDT +GNKRE RSY EIS+S+GVD PSE+
Sbjct: 301 VYIYSSGSREAQRLIFGNSNYGDLRKYLCGFFDTTIGNKRESRSYFEISQSVGVDNPSEV 360

Query: 361 LFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDI 394
           LFITDVYQEA+AAK AGL V ISIRPGNGPLP++HGF+T  SF +I
Sbjct: 361 LFITDVYQEAIAAKEAGLDVIISIRPGNGPLPENHGFKTVGSFLEI 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
MTBC1_VITVI6.9e-11978.23Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
MTBC_POPTR1.5e-11878.11Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
MTBC_RICCO2.9e-11777.37Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
MTBC_ORYSJ1.1e-10373.39Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
MTBC_ORYSI1.1e-10373.39Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
Match NameE-valueIdentityDescription
A0A0A0LAF4_CUCSA1.5e-17678.23Uncharacterized protein OS=Cucumis sativus GN=Csa_3G233480 PE=4 SV=1[more]
A0A059BMC0_EUCGR1.3e-12962.03Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F01149 PE=4 SV=1[more]
A0A059BMP2_EUCGR6.2e-12758.02Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F01149 PE=4 SV=1[more]
A0A059BNB1_EUCGR1.9e-12059.29Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F01149 PE=4 SV=1[more]
A0A0D2SFC4_GOSRA2.0e-11775.71Uncharacterized protein OS=Gossypium raimondii GN=B456_013G170700 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G53850.27.8e-10575.21 haloacid dehalogenase-like hydrolase family protein[more]
Match NameE-valueIdentityDescription
gi|659112020|ref|XP_008456026.1|1.5e-18283.72PREDICTED: probable bifunctional methylthioribulose-1-phosphate dehydratase/enol... [more]
gi|659112018|ref|XP_008456025.1|2.2e-17878.71PREDICTED: probable bifunctional methylthioribulose-1-phosphate dehydratase/enol... [more]
gi|778680342|ref|XP_011651293.1|2.1e-17678.23PREDICTED: probable bifunctional methylthioribulose-1-phosphate dehydratase/enol... [more]
gi|747100405|ref|XP_011098291.1|3.3e-13761.94PREDICTED: probable bifunctional methylthioribulose-1-phosphate dehydratase/enol... [more]
gi|720031130|ref|XP_010265717.1|2.3e-13563.79PREDICTED: probable bifunctional methylthioribulose-1-phosphate dehydratase/enol... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001303Aldolase_II/adducin_N
IPR023214HAD_sf
IPR023943Enolase-ppase_E1
Vocabulary: Molecular Function
TermDefinition
GO:0000287magnesium ion binding
GO:0043874acireductone synthase activity
Vocabulary: Biological Process
TermDefinition
GO:0019509L-methionine biosynthetic process from methylthioadenosine
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019509 L-methionine biosynthetic process from methylthioadenosine
biological_process GO:0019284 L-methionine biosynthetic process from S-adenosylmethionine
biological_process GO:0006470 protein dephosphorylation
biological_process GO:0016311 dephosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0005829 cytosol
cellular_component GO:0005575 cellular_component
molecular_function GO:0043715 2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity
molecular_function GO:0043716 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity
molecular_function GO:0043874 acireductone synthase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0046570 methylthioribulose 1-phosphate dehydratase activity
molecular_function GO:0008270 zinc ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G003990.1CmaCh16G003990.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001303Class II aldolase/adducin N-terminalGENE3DG3DSA:3.40.225.10coord: 29..105
score: 3.
IPR023214HAD-like domainGENE3DG3DSA:3.40.50.1000coord: 151..369
score: 1.3
IPR023214HAD-like domainunknownSSF56784HAD-likecoord: 151..393
score: 4.46
IPR023943Enolase-phosphatase E1TIGRFAMsTIGR01691TIGR01691coord: 152..392
score: 5.5
NoneNo IPR availablePANTHERPTHR20371FAMILY NOT NAMEDcoord: 133..393
score: 4.0E
NoneNo IPR availablePANTHERPTHR20371:SF1ENOLASE-PHOSPHATASE E1coord: 133..393
score: 4.0E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh16G003990Cucsa.032540Cucumber (Gy14) v1cgycmaB0007
CmaCh16G003990Cucsa.123090Cucumber (Gy14) v1cgycmaB0321
CmaCh16G003990Cla023378Watermelon (97103) v1cmawmB325
CmaCh16G003990Cla004295Watermelon (97103) v1cmawmB317
CmaCh16G003990Csa3G233480Cucumber (Chinese Long) v2cmacuB320
CmaCh16G003990Csa4G637120Cucumber (Chinese Long) v2cmacuB340
CmaCh16G003990MELO3C019407Melon (DHL92) v3.5.1cmameB298
CmaCh16G003990ClCG05G009310Watermelon (Charleston Gray)cmawcgB297
CmaCh16G003990ClCG11G018020Watermelon (Charleston Gray)cmawcgB288
CmaCh16G003990CSPI03G19420Wild cucumber (PI 183967)cmacpiB326
CmaCh16G003990CSPI04G23420Wild cucumber (PI 183967)cmacpiB345
CmaCh16G003990CmoCh16G004330Cucurbita moschata (Rifu)cmacmoB322
CmaCh16G003990Lsi04G020670Bottle gourd (USVL1VR-Ls)cmalsiB311
CmaCh16G003990Lsi05G001410Bottle gourd (USVL1VR-Ls)cmalsiB316
CmaCh16G003990Cp4.1LG14g03820Cucurbita pepo (Zucchini)cmacpeB344
CmaCh16G003990MELO3C019407.2Melon (DHL92) v3.6.1cmamedB346
CmaCh16G003990CsaV3_3G019470Cucumber (Chinese Long) v3cmacucB0373
CmaCh16G003990CsaV3_4G033640Cucumber (Chinese Long) v3cmacucB0396
CmaCh16G003990Cla97C11G224060Watermelon (97103) v2cmawmbB340
CmaCh16G003990Cla97C05G091630Watermelon (97103) v2cmawmbB347
CmaCh16G003990Bhi09G002808Wax gourdcmawgoB0451
CmaCh16G003990Bhi01G001165Wax gourdcmawgoB0438
CmaCh16G003990CsGy4G021770Cucumber (Gy14) v2cgybcmaB500
CmaCh16G003990CsGy3G019330Cucumber (Gy14) v2cgybcmaB332
CmaCh16G003990Carg11331Silver-seed gourdcarcmaB0924
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh16G003990Cucurbita pepo (Zucchini)cmacpeB351
CmaCh16G003990Silver-seed gourdcarcmaB0053
CmaCh16G003990Cucurbita maxima (Rimu)cmacmaB351
CmaCh16G003990Cucurbita moschata (Rifu)cmacmoB338