CmaCh16G003030 (gene) Cucurbita maxima (Rimu)

NameCmaCh16G003030
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionPyruvate kinase
LocationCma_Chr16 : 1410722 .. 1414871 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAGGTAAATGCTATGATGATTCGTGCTGGTTGCAATTTTGTTGCGAAAAAGCCTGTATTAGATGTGAAGCGTTCGGGTTTTGGCATACCGGTTTTCGGTCGAACAGTTTGGTGCAATCGAAGGAGGACGTCGGGGAGAGTTGGAAATGGGGTGAAAGCGGTAATGCAAGTGGATTTGGAGGATGCGGAGAGATTGAGAAGATTGGAGGGCTTAGAAGCGACATTAGGGCTTGATGTTGTAGCGGAAAGGGAGTTGAAGGAGAAAGGATTTTTAGGGATGAGGAAGACGAAGCTTGTATGTACTATTGGCCCTGCGTGTAGTGCGATTGAGGATCTGGAGAAGTTGGCATTGGAAGGTATGAACATTGCGAGATTGAATATGTGCCACAATTCGAGAGAGTGGCATTGCGATGTGATTAGGAAGATCAAGAAGTTAAATGAGGGGAAAGGATTCTGTATTTCGGTGATGATCGATACTGAAGGGAGTCAGATTCATGTGGTTGATCATGGAGCTCCTTCCTCCATAAAAGTAGAGGTAAATGTTATGTCTCTGTTATTTTTTGCTGCTATTTAACTGAAATATGTTACTATGTTCACTGTTTTCTTCTGTAAATTAAGAACACTGAGTCTAAATTTTCTGGCGATAATTGTTCAGGATGGTTCAATCTGGTTGTTTACTGCTGAGAAATTTGAGGGCTATCGTTCATTCACTGTTCAAGCGAGCTATGAAGGTTTCTCTGAAGGTGTGTCTTGAATCTCTTGTTTCTTCGTCTTCTGAATTGTGATTAGAAGAAATATTTGAATCCCACAAGTTACTTAGAATATGAGTTTGATGTTGATGAGCTTTCTTTTACGAGTTTGGGCAGGTATTCAAGTGGGTGATTCAGTTGTAATTGATGGAGGAATGGCAACCTTTGAGGTTATTGAAAAGATTGGGAATGATTTAAGCTGCAAGTGCACAGACCCTGGTTTGTTTCTTCCTCGAGCAAAGTACAGCTTTTGGAGAGATGGGAGGGTTGTTGTGAGAAATCACGAGTCTCCAACGCTGTCGAGTAAGGTAGGAACGTGTCTCTGCTGCATATCAATACTCATTCTGAGAAATTTAGTTGTAATGAAATATGTGAGTTGATTTTAGTGTGAGATTTCATCTTGGCGATGAGAACGAAACATTTCTTATAAGGGGAGAAAATCTCTCCCTAGTAGACACGTTTTAAAACCGTGAATTTGATGACGATATATAATGGGCTAAAGCAGACAATATCTACTAGCAGTGGATTTGGGCTGTTGCGAATGGTATCAGAGCCAAATACTGAGCGGTGTGCTAACAAGGACGTTGGGCTACCAATGGGAGTGGATTGTGAGATTCCACATCAGTTGGAGAAGGGAAACCTCTCTCTAGTAAACACATTTTAAAACCATGAGGTTGACGGCGATACGAACGGGCAAAAGCGGACAATATCTGCTAGCGGTGGGTTTGGACTGTTACAAATGGTATTAGAGCCAAACACCCGGTGGTGTGCCAACAAGGACGTTGGCCCCCAAGAAGTGGATGGTGAGATTCCACATCGGTTGGAGAGGGGAACAAAGTATTCCTTGTAAGGGTGTAGAAACCTCAAAAGTATTCCTTGTAAGGGTGTAGAAACCTCTTTCTAGTAGACGCGTTTTAGAACCGTGAGCCTGATAGTGATACATAACGGGCCAAAGCGAACAATATCTACTAGCGGTGGGTTTGGGTGTTACATTTATGAAGTTGAAACTTTAAGATATGATGTATTTTATTGATTGGCCTTTTCATATTAATGCATAAACATGAGTAAAAGAATTCAATTAATCATGTCTTCTTGGATGATGCCGGATCTCTGCGTTTAAACCCTGTTTTTTCATGGTGTATTACAGGATTGGTCGGACATTGAATTTGGCATTGCGGAAGGCGTCAACTTCATTGCCCTTTCCTTCGTGAACGGTGCTGATCCTGTCAAGCATCTAAAGAACTACCTATCAACAAAATCAGCAAAGTAAGCATAAGAAGTCAACTTTTACCATGAATTGGGTTGAACTATCACTATCACTATCACTACCACTACTATGCATGAACGATAAATTATAGAGACATTCTTTTCCATAAAAATTTCCGGCAAAATATTGCTACTGAAACAGATATTTTGGGTAATTTCATAACTCAATTTAGTGATGAAGGGGGAAATCTGTTTTCATGCACAGATCCGTAAGGGTATTGGCTAAGATTGAAAGCTTGGAGTCTCTTCAGAATCTGGAAGAAATTATAGAGGCCTCTGATGGAATCATGGTGGCTCGAGGAGATCTTGGGGTTGAAATTCCGCTGGAGCAGATACCAGCAATCCAAGAGGAAATAACTAGTGTTTGTAGGGAACTGAACAAACCAGTGATCATAGCTTCCCAACTTCTAGAGTCGATGGTTGAATATCCTACTCCAACACGAGCTGAGGTATGAAAAATTTATCTTCGGTTTCTAATTTCAGGCATGTTTGGAATGATTTTCAAAATGTTGAAGACTATAATGATGATCCATTTTGGTGGGGTGTTGAATAGGTTGCAGATGTTTCGGAGGCTGTCCGACAGTATGCTGATGCATTAATGTTATCTGGGGAGTCTGCGATAGGATCATATGGACAGAAAGCGCTTTCGGTTCTACAGATGGCCAGCTGCCGAATGGAGTTATGGAGTCGCGAAGAAAATAGGGAAAACTTCCTTCCCCACTATCAACTTGGGGTGTCATTGCACGATCGCATTGCAGAAGAAATTTGCAATTCTGCTTCTGAATTAGGTAAAACAGTTTGGTTTTTGGCTTTTATTCAATAATTCTTTATAGAATATATTGGCCTGTCTTAAATTCTAAAATATTTATATTTCAAGAATAGTCTTAAAACCTTCGTAAAAGTGAAGAGTAATTTTTACAAGCACAAGAATAGCTTCTAAAAGTCCATGAACCTGTGTGAAACTTGCGTATGGGAGGCCGTTAGAGATATTTAGTTTAGTTCCTAAATTTTTAGGTTTGTATTTATTGGTCGCGGGCCTTTCAAAAAAATGTCTAAAAGGTTCTTAAACTTTCAATTATGTATCCAATAAGATCTTGAACTTTAAAAAGTGTGTAATATATTCATAATCTTTCTTTTGTATGTTTGATAAGTTCATTAACTTTAAAAACAAATATGTGTATGATAAATCTTTTAATTGTGTATGATAAATCTTTTAATTTTCTAATTTTGTATTTAATAGGTCGTAGCATATTTTTAAGTTCGACGAATTAATTAGGCAAATGTTTTTGTGGTATAAAGCTGTTGTAGAACTGATCCTTGCACTTAGTTAGGTGTCAAGAAGTAAATTAATTTACCTTTGAACGAAAACTACTGTTTTGTTTGAGTGTTGCTAATTGCATTTGGCCCACGTTTCCACCAAAACAAACATCTCCATGTTGTTTTTGGCAGCTAACAGGCTTTCAGTAGACGCCATATTTGTGCTCACAACGCAAGGACACATGGCATCTCTTCTATCCCGCAACCGCCCAAGGCCGCCTATATTTGCATTGACCGATGATGATAGCACAAGAATGGCTTTGAATCTGCAATGGGGAGTGTTTCCTCTGCGCATTGAGCTCTCAGAAGACATTGAAGCAAACATCTGGAGGGGCATCGAGCTTGTGAAATCGAAGGGGTTGGTGAAACAGGGGGACTCTGTGTTGGTGGTCTCTGAAATCTCTCCAGCTCGAGCTGCATCAATGGCGTCCCAATCAATCCAGCTGAAAACCATCGCGTAGGAGAAAGAGAAGAAGAGAACCAACCAATCCAACAAGAAACTTCAGTGATTTATGATTGCATGTACCATATGTGGAGGTTATTTATCTACTTATTATTTGTGGCCCTCTGTTTATAAAGACTACTTCGAAGCTTGGATAGGCCGTTTGTTGTTTTGTACTACTAGTAGCTAGTATTTGGTCATTTGAATAACTGTACTGAAATTGGGTATTCTGGATTTATTTGGTAGAAACAGGAGACGTAGCGAGTTAGGTTACTGAGAGAAATCTTCTTCCCTTAGTTTATTCCAATCGATTGAGTAGTCGTAGAAGTCGCATTCAAGCTACCGTCCTACTATAAGGATAATAAGCCATA

mRNA sequence

ATGGCGGAGGTAAATGCTATGATGATTCGTGCTGGTTGCAATTTTGTTGCGAAAAAGCCTGTATTAGATGTGAAGCGTTCGGGTTTTGGCATACCGGTTTTCGGTCGAACAGTTTGGTGCAATCGAAGGAGGACGTCGGGGAGAGTTGGAAATGGGGTGAAAGCGGTAATGCAAGTGGATTTGGAGGATGCGGAGAGATTGAGAAGATTGGAGGGCTTAGAAGCGACATTAGGGCTTGATGTTGTAGCGGAAAGGGAGTTGAAGGAGAAAGGATTTTTAGGGATGAGGAAGACGAAGCTTGTATGTACTATTGGCCCTGCGTGTAGTGCGATTGAGGATCTGGAGAAGTTGGCATTGGAAGGTATGAACATTGCGAGATTGAATATGTGCCACAATTCGAGAGAGTGGCATTGCGATGTGATTAGGAAGATCAAGAAGTTAAATGAGGGGAAAGGATTCTGTATTTCGGTGATGATCGATACTGAAGGGAGTCAGATTCATGTGGTTGATCATGGAGCTCCTTCCTCCATAAAAGTAGAGGATGGTTCAATCTGGTTGTTTACTGCTGAGAAATTTGAGGGCTATCGTTCATTCACTGTTCAAGCGAGCTATGAAGGTTTCTCTGAAGGTATTCAAGTGGGTGATTCAGTTGTAATTGATGGAGGAATGGCAACCTTTGAGGTTATTGAAAAGATTGGGAATGATTTAAGCTGCAAGTGCACAGACCCTGGTTTGTTTCTTCCTCGAGCAAAGTACAGCTTTTGGAGAGATGGGAGGGTTGTTGTGAGAAATCACGAGTCTCCAACGCTGTCGAGTAAGGATTGGTCGGACATTGAATTTGGCATTGCGGAAGGCGTCAACTTCATTGCCCTTTCCTTCGTGAACGGTGCTGATCCTGTCAAGCATCTAAAGAACTACCTATCAACAAAATCAGCAAAATCCGTAAGGGTATTGGCTAAGATTGAAAGCTTGGAGTCTCTTCAGAATCTGGAAGAAATTATAGAGGCCTCTGATGGAATCATGGTGGCTCGAGGAGATCTTGGGGTTGAAATTCCGCTGGAGCAGATACCAGCAATCCAAGAGGAAATAACTAGTGTTTGTAGGGAACTGAACAAACCAGTGATCATAGCTTCCCAACTTCTAGAGTCGATGGTTGAATATCCTACTCCAACACGAGCTGAGGTTGCAGATGTTTCGGAGGCTGTCCGACAGTATGCTGATGCATTAATGTTATCTGGGGAGTCTGCGATAGGATCATATGGACAGAAAGCGCTTTCGGTTCTACAGATGGCCAGCTGCCGAATGGAGTTATGGAGTCGCGAAGAAAATAGGGAAAACTTCCTTCCCCACTATCAACTTGGGGTGTCATTGCACGATCGCATTGCAGAAGAAATTTGCAATTCTGCTTCTGAATTAGCTAACAGGCTTTCAGTAGACGCCATATTTGTGCTCACAACGCAAGGACACATGGCATCTCTTCTATCCCGCAACCGCCCAAGGCCGCCTATATTTGCATTGACCGATGATGATAGCACAAGAATGGCTTTGAATCTGCAATGGGGAGTGTTTCCTCTGCGCATTGAGCTCTCAGAAGACATTGAAGCAAACATCTGGAGGGGCATCGAGCTTGTGAAATCGAAGGGGTTGGTGAAACAGGGGGACTCTGTGTTGGTGGTCTCTGAAATCTCTCCAGCTCGAGCTGCATCAATGGCGTCCCAATCAATCCAGCTGAAAACCATCGCGTAGGAGAAAGAGAAGAAGAGAACCAACCAATCCAACAAGAAACTTCAGTGATTTATGATTGCATGTACCATATGTGGAGGTTATTTATCTACTTATTATTTGTGGCCCTCTGTTTATAAAGACTACTTCGAAGCTTGGATAGGCCGTTTGTTGTTTTGTACTACTAGTAGCTAGTATTTGGTCATTTGAATAACTGTACTGAAATTGGGTATTCTGGATTTATTTGGTAGAAACAGGAGACGTAGCGAGTTAGGTTACTGAGAGAAATCTTCTTCCCTTAGTTTATTCCAATCGATTGAGTAGTCGTAGAAGTCGCATTCAAGCTACCGTCCTACTATAAGGATAATAAGCCATA

Coding sequence (CDS)

ATGGCGGAGGTAAATGCTATGATGATTCGTGCTGGTTGCAATTTTGTTGCGAAAAAGCCTGTATTAGATGTGAAGCGTTCGGGTTTTGGCATACCGGTTTTCGGTCGAACAGTTTGGTGCAATCGAAGGAGGACGTCGGGGAGAGTTGGAAATGGGGTGAAAGCGGTAATGCAAGTGGATTTGGAGGATGCGGAGAGATTGAGAAGATTGGAGGGCTTAGAAGCGACATTAGGGCTTGATGTTGTAGCGGAAAGGGAGTTGAAGGAGAAAGGATTTTTAGGGATGAGGAAGACGAAGCTTGTATGTACTATTGGCCCTGCGTGTAGTGCGATTGAGGATCTGGAGAAGTTGGCATTGGAAGGTATGAACATTGCGAGATTGAATATGTGCCACAATTCGAGAGAGTGGCATTGCGATGTGATTAGGAAGATCAAGAAGTTAAATGAGGGGAAAGGATTCTGTATTTCGGTGATGATCGATACTGAAGGGAGTCAGATTCATGTGGTTGATCATGGAGCTCCTTCCTCCATAAAAGTAGAGGATGGTTCAATCTGGTTGTTTACTGCTGAGAAATTTGAGGGCTATCGTTCATTCACTGTTCAAGCGAGCTATGAAGGTTTCTCTGAAGGTATTCAAGTGGGTGATTCAGTTGTAATTGATGGAGGAATGGCAACCTTTGAGGTTATTGAAAAGATTGGGAATGATTTAAGCTGCAAGTGCACAGACCCTGGTTTGTTTCTTCCTCGAGCAAAGTACAGCTTTTGGAGAGATGGGAGGGTTGTTGTGAGAAATCACGAGTCTCCAACGCTGTCGAGTAAGGATTGGTCGGACATTGAATTTGGCATTGCGGAAGGCGTCAACTTCATTGCCCTTTCCTTCGTGAACGGTGCTGATCCTGTCAAGCATCTAAAGAACTACCTATCAACAAAATCAGCAAAATCCGTAAGGGTATTGGCTAAGATTGAAAGCTTGGAGTCTCTTCAGAATCTGGAAGAAATTATAGAGGCCTCTGATGGAATCATGGTGGCTCGAGGAGATCTTGGGGTTGAAATTCCGCTGGAGCAGATACCAGCAATCCAAGAGGAAATAACTAGTGTTTGTAGGGAACTGAACAAACCAGTGATCATAGCTTCCCAACTTCTAGAGTCGATGGTTGAATATCCTACTCCAACACGAGCTGAGGTTGCAGATGTTTCGGAGGCTGTCCGACAGTATGCTGATGCATTAATGTTATCTGGGGAGTCTGCGATAGGATCATATGGACAGAAAGCGCTTTCGGTTCTACAGATGGCCAGCTGCCGAATGGAGTTATGGAGTCGCGAAGAAAATAGGGAAAACTTCCTTCCCCACTATCAACTTGGGGTGTCATTGCACGATCGCATTGCAGAAGAAATTTGCAATTCTGCTTCTGAATTAGCTAACAGGCTTTCAGTAGACGCCATATTTGTGCTCACAACGCAAGGACACATGGCATCTCTTCTATCCCGCAACCGCCCAAGGCCGCCTATATTTGCATTGACCGATGATGATAGCACAAGAATGGCTTTGAATCTGCAATGGGGAGTGTTTCCTCTGCGCATTGAGCTCTCAGAAGACATTGAAGCAAACATCTGGAGGGGCATCGAGCTTGTGAAATCGAAGGGGTTGGTGAAACAGGGGGACTCTGTGTTGGTGGTCTCTGAAATCTCTCCAGCTCGAGCTGCATCAATGGCGTCCCAATCAATCCAGCTGAAAACCATCGCGTAG

Protein sequence

MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVDLEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA
BLAST of CmaCh16G003030 vs. Swiss-Prot
Match: PKP1_ARATH (Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana GN=PKP1 PE=1 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 1.1e-168
Identity = 292/487 (59.96%), Postives = 369/487 (75.77%), Query Frame = 1

Query: 79  LDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHC 138
           +D V E ELKE GF   R+TKL+CTIGPA    E LE LA+ GMN+ARLNMCH +R+WH 
Sbjct: 100 VDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHR 159

Query: 139 DVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGYR-S 198
            VIR +++LNE KGF +++M+DTEGS+IH+ D G  +S K EDG +W FT   F+  R  
Sbjct: 160 GVIRSVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPE 219

Query: 199 FTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYSFWRD 258
            T+  SY+GF+E ++VGD +++DGGM  FEVIEKIG D+ C CTDPGL LPRA  +FWRD
Sbjct: 220 RTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRD 279

Query: 259 GRVV-VRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKS-AKSV 318
           G +V  RN   PT+SSKDW DI+FGIAEGV+FIA+SFV  A+ + HLK+YL+ +S    +
Sbjct: 280 GSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEI 339

Query: 319 RVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVI 378
            V+AKIES++SL NLEEII ASDG MVARGDLG +IPLEQ+PA Q+ I  VCR LNKPVI
Sbjct: 340 GVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVI 399

Query: 379 IASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRM 438
           +ASQLLESM+EYPTPTRAEVADVSEAVRQ +DALMLSGESA+G +  KAL+VL+  S R+
Sbjct: 400 VASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRI 459

Query: 439 ELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLS 498
           E W REE R   +P   +G S  D+I+EEICNSA+++AN L VDA+FV TT GHMASL+S
Sbjct: 460 ERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVS 519

Query: 499 RNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDS 558
           R RP  PIFA T   S R  LNLQWG+ P R+  S+D+E+N+ +   L+KS+G++K GD 
Sbjct: 520 RCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDL 579

Query: 559 VLVVSEI 563
           V+ VS++
Sbjct: 580 VIAVSDM 586

BLAST of CmaCh16G003030 vs. Swiss-Prot
Match: KPYA_RICCO (Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis PE=1 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 2.6e-167
Identity = 287/491 (58.45%), Postives = 374/491 (76.17%), Query Frame = 1

Query: 75  ATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSR 134
           +++ +D V E ELKE GF   R+TKLVCTIGPA    E+LE LA+ GMN+AR+NMCH +R
Sbjct: 83  SSIEVDAVTETELKENGFRSTRRTKLVCTIGPATCGFEELEALAVGGMNVARINMCHGTR 142

Query: 135 EWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEG 194
           EWH  VI ++++LNE KGF +++M+DTEGS+IH+ D G  SS K EDG IW F+   ++ 
Sbjct: 143 EWHKSVIERVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAYDS 202

Query: 195 YR-SFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYS 254
            R   T+  +Y+GF+E ++VGD +++DGGM  FEVIEKIG D+ C+CTDPGL LPRA  +
Sbjct: 203 PRPERTINVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCRCTDPGLLLPRANLT 262

Query: 255 FWRDGRVV-VRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSA 314
           FWRDG +V  RN   PT+SSKDW DI+FGIAEGV+FIA+SFV  A+ + HLK+Y++ +S 
Sbjct: 263 FWRDGSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAISFVKSAEVINHLKSYIAARSR 322

Query: 315 KS-VRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELN 374
            S + V+AKIES++SL+NLEEII ASDG MVARGDLG +IPLEQ+P+ Q+ I  VCR+LN
Sbjct: 323 DSDIAVIAKIESIDSLKNLEEIIRASDGAMVARGDLGAQIPLEQVPSAQQNIVQVCRQLN 382

Query: 375 KPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMA 434
           KPVI+ASQLLESM+EYPTPTRAEVADVSEAVRQ ADALMLSGESA+G Y +KAL+VL+  
Sbjct: 383 KPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRADALMLSGESAMGQYPEKALAVLRSV 442

Query: 435 SCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMA 494
           S R+E W REE     +    +G +  D I+EEICNSA+++AN L VDA+FV T  GHMA
Sbjct: 443 SVRIEKWWREEKHHEAMELPAIGSTYSDSISEEICNSAAKMANNLGVDALFVYTKDGHMA 502

Query: 495 SLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVK 554
           SLLSR RP  PIFA T   S R  LNLQWG+ P R+  ++D+E+N+ +   L+K++G++K
Sbjct: 503 SLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFADDMESNLNKTFSLLKARGMIK 562

Query: 555 QGDSVLVVSEI 563
            GD V+ VS++
Sbjct: 563 SGDLVIAVSDM 573

BLAST of CmaCh16G003030 vs. Swiss-Prot
Match: KPYA_TOBAC (Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 3.0e-163
Identity = 282/491 (57.43%), Postives = 371/491 (75.56%), Query Frame = 1

Query: 75  ATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSR 134
           +++ +D V E ELKE GF   R+TKL+CTIGPA    E LE+LA  GMN+AR+NMCH +R
Sbjct: 93  SSIEVDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLERLAEGGMNVARINMCHGTR 152

Query: 135 EWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEG 194
           EWH  VI ++++LNE KGF +++M+DTEGS+IH+ D G  SS K EDG IW FT   F+ 
Sbjct: 153 EWHRMVIERLRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWNFTVRSFDP 212

Query: 195 -YRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYS 254
                TV  +Y+GF+E ++VGD +++DGGM  FEVIEKIG D+ C CTDPGL LPRA  +
Sbjct: 213 PLPERTVTVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLT 272

Query: 255 FWRDGRVV-VRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSA 314
           FWRDG++V  RN   PT+SSKDW DI+FGIAEGV+FIA+SFV  A+ +KHLK+Y+  ++ 
Sbjct: 273 FWRDGKLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVIKHLKSYIQARAR 332

Query: 315 KS-VRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELN 374
            S + V+AKIES++SL+NLEEII+ASDG MVARGDLG +IPLEQ+P+ Q++I  +CR+LN
Sbjct: 333 DSDISVIAKIESIDSLKNLEEIIQASDGAMVARGDLGAQIPLEQVPSEQQKIVQICRQLN 392

Query: 375 KPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMA 434
           +PVI+ASQLLESM+EYP PTRAEVADVSEAVRQ  DALMLSGESA+G + +KAL+VL+  
Sbjct: 393 RPVIVASQLLESMIEYPIPTRAEVADVSEAVRQRGDALMLSGESAMGQFPEKALTVLRSV 452

Query: 435 SCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMA 494
           S R+E   RE+ R   +    +  S  D I+EEICNSA+++AN L VDA+FV T  GHMA
Sbjct: 453 SLRIERMWREQKRHEVIELPSIASSFSDSISEEICNSAAKMANNLEVDALFVYTKNGHMA 512

Query: 495 SLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVK 554
           SLLSR RP  PIFA T   S R  LNLQWG+ P R+  S+D+E+N+ +   L+K++G++K
Sbjct: 513 SLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLMPFRLSFSDDMESNLNKTFSLLKARGMIK 572

Query: 555 QGDSVLVVSEI 563
            GD ++ VS++
Sbjct: 573 SGDLIIAVSDM 583

BLAST of CmaCh16G003030 vs. Swiss-Prot
Match: KPYG_TOBAC (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 8.6e-86
Identity = 187/465 (40.22%), Postives = 274/465 (58.92%), Query Frame = 1

Query: 96  RKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHCDVIRKIKKLN-EGKGFC 155
           RKTK+VCTIGP+ S+ E + KLA  GMN+ARLNM H     H   I  +K+ N + +   
Sbjct: 91  RKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFEDKV 150

Query: 156 ISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVG 215
           I++M+DT+G +  V+    P  I +++G  + F+ ++       TV  +Y+ F   ++ G
Sbjct: 151 IAIMLDTKGPE--VISGDVPKPILLKEGQEFNFSIKRGVSTED-TVSVNYDDFINDVEAG 210

Query: 216 DSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKD 275
           D +++DGGM +  V  K  + + C+  D G    R      R   V  ++   P+++ KD
Sbjct: 211 DILLVDGGMMSLAVKSKTSDIVKCEVIDGGELKSR------RHLNVRGKSATLPSITEKD 270

Query: 276 WSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSAKSVRVLAKIESLESLQNLEEII 335
           W DI+FG+   V+F A+SFV  A  V  LK+YL + +A  + V+ KIES +S+ NL  II
Sbjct: 271 WDDIKFGVNNQVDFYAVSFVKDAKVVHELKDYLKSCNA-DIHVIVKIESADSIPNLHSII 330

Query: 336 EASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVIIASQLLESMVEYPTPTRAE 395
            ASDG MVARGDLG E+P+E++P +QE+I   C+ + KPVI+A+ +LESM+++PTPTRAE
Sbjct: 331 SASDGAMVARGDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMIDHPTPTRAE 390

Query: 396 VADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRMELWSREENRENFLPHYQLG 455
           V+D+S AVR+ ADA+MLSGE+A G Y  KA+ V+ + + R E       +    P     
Sbjct: 391 VSDISIAVREGADAVMLSGETAHGKYPLKAVKVMHIVALRTE---SSLQKSTSSPSQSAA 450

Query: 456 VSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRM 515
              H  + E     +S +AN LS   I V T  G MA +LS NRP   +FA T+++  + 
Sbjct: 451 YKSH--MGEMFAFHSSSMANTLST-PIIVFTRTGSMAIILSHNRPSSTVFAFTNNERVKQ 510

Query: 516 ALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDSVLVV 560
            L L  GV P+ +E S D E    R I+L+ SK LVK G  V +V
Sbjct: 511 RLALYHGVVPIYMEFSSDAEETFSRAIKLLLSKSLVKDGQYVTLV 539

BLAST of CmaCh16G003030 vs. Swiss-Prot
Match: PKP2_ARATH (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana GN=PKP2 PE=1 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 3.6e-84
Identity = 200/575 (34.78%), Postives = 310/575 (53.91%), Query Frame = 1

Query: 24  VKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVDLEDAERLRRLEGLEATLGLDVVA 83
           +K + F + V G     N  + SGRV    + V+   +  A    R+E     +  + V 
Sbjct: 30  LKPASFAVKVLG-----NEAKRSGRVSVRSRRVVDTTVRSA----RVETEVIPVSPEDVP 89

Query: 84  ERELKEKGFLGM-----------------RKTKLVCTIGPACSAIEDLEKLALEGMNIAR 143
            RE + +  L M                 RKTK+VCT+GP+ +  E + KLA  GMN+AR
Sbjct: 90  NREEQLERLLEMQQFGDTSVGMWSKPTVRRKTKIVCTVGPSTNTREMIWKLAEAGMNVAR 149

Query: 144 LNMCHNSREWHCDVIRKIKKLN-EGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIW 203
           +NM H     H  VI  +K+ N + K   I++M+DT+G ++   D   P  I ++ G  +
Sbjct: 150 MNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTKGPEVRSGD--LPQPIMLDPGQEF 209

Query: 204 LFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGL 263
            FT E+     S  V  +Y+ F   ++ GD +++DGGM +F V  K  + + C+  D G 
Sbjct: 210 TFTIERGVSTPS-CVSVNYDDFVNDVEAGDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGE 269

Query: 264 FLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKN 323
              R      R   V  ++   P+++ KDW DI+FG+   V+F A+SFV  A  V  LK 
Sbjct: 270 LKSR------RHLNVRGKSATLPSITEKDWEDIKFGVENKVDFYAVSFVKDAQVVHELKK 329

Query: 324 YLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITS 383
           YL    A  + V+ KIES +S+ NL  II ASDG MVARGDLG E+P+E++P +QEEI +
Sbjct: 330 YLQNSGA-DIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPILQEEIIN 389

Query: 384 VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKAL 443
           +CR + K VI+A+ +LESM+ +PTPTRAEV+D++ AVR+ ADA+MLSGE+A G +  KA 
Sbjct: 390 LCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAA 449

Query: 444 SVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLT 503
            V+   + R E           +P   LG +  + ++E     A+ ++N L    + V T
Sbjct: 450 GVMHTVALRTEATITSGE----MPP-NLGQAFKNHMSEMFAYHATMMSNTLGTSTV-VFT 509

Query: 504 TQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVK 563
             G MA LLS  RP   I+A T++   +  L L  GV P+ +E ++D E      +  + 
Sbjct: 510 RTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGVCPIYMEFTDDAEETFANALATLL 569

Query: 564 SKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 581
            +G+VK+G+ + +V   +     S ++ +IQ++ +
Sbjct: 570 KQGMVKKGEEIAIVQSGTQPIWRSQSTHNIQVRKV 579

BLAST of CmaCh16G003030 vs. TrEMBL
Match: E5GBH7_CUCME (Pyruvate kinase OS=Cucumis melo subsp. melo PE=3 SV=1)

HSP 1 Score: 1043.9 bits (2698), Expect = 7.4e-302
Identity = 529/581 (91.05%), Postives = 553/581 (95.18%), Query Frame = 1

Query: 1   MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFG-RTVWCNRRRTSGRVGNGVKAVMQV 60
           MAEVNAMMIRA CNF+ + PVLDVKR GF +PV G RTVWCNRRR  GRVGNGVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120
           DL+D ERLRRLEGLEATLGLD+VAERELKEKGFLGMRKTKLVCTIGPACS IEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKV 180
           EGMNIARLNMCHNSREWHCD+I+KIKKLNE KGFCISVMID EGS+IHVVDHGAPSS+KV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCK 240
           EDGSIWLFTAEKFEG R FTVQASYEGFSEGI+VGDS+VIDGGMATFEV EKIGNDLSC+
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADP 300
           CTDPGLFLPRAK SFWRDGR+VVRNHESPTLSSKDWSDIEFGI+EGV+FIALSFVN ADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VKHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAI 360
           V+HLKNYL TKSAKS+RVLAKIESLE+LQNLEEIIEASDGIMVARGDLGVEIPLEQIPA+
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVD 480
           YGQKALSVLQMAS RMELWSREEN +NFLP +QLGVSLHDRIAEEICNSA+ELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWR 540
           AIFVLT QGHMASLLSRNRP PPIFALTDDDSTRMALNLQWGVFPLRI+LSEDIEANI R
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 581
           GIE+VKSKGLVKQGDSVLVVSEISPARA SMASQSIQLKTI
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTI 581

BLAST of CmaCh16G003030 vs. TrEMBL
Match: A0A0A0KSY4_CUCSA (Pyruvate kinase OS=Cucumis sativus GN=Csa_4G000560 PE=3 SV=1)

HSP 1 Score: 1039.6 bits (2687), Expect = 1.4e-300
Identity = 526/581 (90.53%), Postives = 553/581 (95.18%), Query Frame = 1

Query: 1   MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFGR-TVWCNRRRTSGRVGNGVKAVMQV 60
           MAEVNAMMIRA CNF+ + PVLDVKR GF +PV GR TVWC+RRR  GRV NGVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVVNGVKATMQV 60

Query: 61  DLEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120
           DLED ERL+RLEGLEATLGLD+VAERELKEKGFLGMRKTKLVCT+GPACS I DLEKLAL
Sbjct: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKV 180
           EGMNIARLNMCHNS EWHCD+I+KIKKLNE KGFCISVMID EGSQIHVVDHGAPSS+KV
Sbjct: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCK 240
           EDGSIWLFTA+KFEG R FTVQASYEGFSEGI+VGDS+VIDGGMATFEVIEKIGNDLSC+
Sbjct: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADP 300
           CTDPGLFLPRAK SFWRDGR+V+RNHESPTLSSKDWSDIEFGI+EGV+FIALSFVN ADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VKHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAI 360
           V+HLKNYLSTKSAKS+RVLAKIESLE+LQNLEEI+EASDGIMVARGDLGVEIPLEQIPA+
Sbjct: 301 VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVD 480
           YGQKALSVLQMAS RMELWSREENR NFLP +QLGVSLHDRIAEEICNSA+ELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWR 540
           AIFVLT QGHMASLLSRNRP PPIFALTDDDSTRMALNLQWGVFPLRI+LSEDIEANI R
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 581
           GIE+VKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 581

BLAST of CmaCh16G003030 vs. TrEMBL
Match: M5VME8_PRUPE (Pyruvate kinase OS=Prunus persica GN=PRUPE_ppa003382mg PE=3 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 1.5e-241
Identity = 426/565 (75.40%), Postives = 491/565 (86.90%), Query Frame = 1

Query: 17  AKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVDLEDAERLRRLEGLEAT 76
           +K P +++++  FG+PV    V C  ++  G+VG GV+AV QV LE++ER+R L  LE T
Sbjct: 16  SKNPRVNLEKQVFGVPVLHHGVSCIGKKLKGKVGAGVQAV-QVGLEESERVRNLRSLERT 75

Query: 77  LGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREW 136
           L LD V+EREL+EKGFLG+RKTKLVCTIGPACS++EDLEKL L GMN+ARLNMCHN+REW
Sbjct: 76  LELDAVSERELREKGFLGLRKTKLVCTIGPACSSLEDLEKLVLGGMNVARLNMCHNTREW 135

Query: 137 HCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGYR 196
           H DVIRKIKKLNE KGF +SVMIDTEGSQIHVVDHGAPSS+KV++ SIWLFT EKF+G  
Sbjct: 136 HRDVIRKIKKLNEEKGFSVSVMIDTEGSQIHVVDHGAPSSLKVQEDSIWLFTNEKFDGSV 195

Query: 197 SFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYSFWR 256
            FTVQA YEGFSEGI+VGD +VIDGGMA FEVIEK+ +DL CKCTDPGLFLPRAK+SFWR
Sbjct: 196 PFTVQAKYEGFSEGIKVGDELVIDGGMARFEVIEKLRSDLRCKCTDPGLFLPRAKFSFWR 255

Query: 257 DGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSAKSVR 316
           DG++V RN+E PTLS+KDWSDIEFGI+EGVNFIA+SFVN A+ V+HLKNYLSTKSA S+R
Sbjct: 256 DGKLVERNYELPTLSTKDWSDIEFGISEGVNFIAMSFVNDAESVRHLKNYLSTKSATSIR 315

Query: 317 VLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVII 376
           VLAKIESLESLQ LEEIIEASDGIMVAR DLGVEIPLEQIP +QEEIT VCR+LNKPVI+
Sbjct: 316 VLAKIESLESLQKLEEIIEASDGIMVARVDLGVEIPLEQIPTVQEEITKVCRQLNKPVIV 375

Query: 377 ASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRME 436
           ASQ LESM+EYPTPTRAEVAD+SEAVRQYADALML+GESAIG +GQKALSVLQM S RME
Sbjct: 376 ASQFLESMIEYPTPTRAEVADISEAVRQYADALMLAGESAIGLFGQKALSVLQMTSSRME 435

Query: 437 LWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLSR 496
             SREENR+  L   QLGVSL DRIAE+ICNSA ELAN L+VDAIFV T  GHMASLLSR
Sbjct: 436 SSSREENRQGSLLPRQLGVSLPDRIAEQICNSAVELANNLAVDAIFVYTKHGHMASLLSR 495

Query: 497 NRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDSV 556
           NRP PPIFA T+D+STRM+LNLQWGV PL ++LS+D+E+NI   I L+KSKGLVK+GDSV
Sbjct: 496 NRPNPPIFAFTNDESTRMSLNLQWGVIPLLVDLSDDMESNISSTIHLIKSKGLVKEGDSV 555

Query: 557 LVVSEISPARAASMASQSIQLKTIA 582
           LVVS+++P+RA  MA QSIQ+KTIA
Sbjct: 556 LVVSDVTPSRATPMAFQSIQVKTIA 579

BLAST of CmaCh16G003030 vs. TrEMBL
Match: W9R7Y8_9ROSA (Pyruvate kinase OS=Morus notabilis GN=L484_003357 PE=3 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 3.9e-234
Identity = 405/543 (74.59%), Postives = 472/543 (86.92%), Query Frame = 1

Query: 38  VWCNRRRTSGRVGNGVKAVMQVDLEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRK 97
           V CN R+    V  GV+A++QV LED+E +R+L  LE  L LDV  EREL+EKGFLG+RK
Sbjct: 36  VACNGRKLKRNVFCGVQAMVQVGLEDSESVRKLRVLEGNLELDVALERELREKGFLGLRK 95

Query: 98  TKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISV 157
           TKLVCT+GP CS++EDLE+LAL GMN+ARLNMCHN+REWHCDVIR IK+LNE KGFC+SV
Sbjct: 96  TKLVCTVGPTCSSLEDLERLALGGMNVARLNMCHNTREWHCDVIRNIKRLNEEKGFCVSV 155

Query: 158 MIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSV 217
           MIDTEGSQIHVVDHG+PSS+KVE+GS+WLFTA KF+G R  TVQA+ EGFSEGI+VGD +
Sbjct: 156 MIDTEGSQIHVVDHGSPSSVKVEEGSVWLFTARKFDGSRPLTVQANDEGFSEGIKVGDEL 215

Query: 218 VIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSD 277
           VIDGGMA FEVIEKIG+DL C+C+D GLFLP AK+SFWRDGR+V RNHE PTLS KDW+D
Sbjct: 216 VIDGGMACFEVIEKIGSDLHCRCSDSGLFLPGAKFSFWRDGRLVQRNHELPTLSEKDWAD 275

Query: 278 IEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEAS 337
           IEFGI+EG++F+A+SFVN AD +++LKNY+STKS++ +RVLAKIESLESLQ LEEI+EAS
Sbjct: 276 IEFGISEGIDFVAMSFVNDADSIRNLKNYISTKSSRPIRVLAKIESLESLQKLEEIVEAS 335

Query: 338 DGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVAD 397
           DGIMVARGDLGVEIPLE+IP +Q EI  VCR+LNKPVI+ASQLLESMVEYPTPTRAEVAD
Sbjct: 336 DGIMVARGDLGVEIPLEKIPTVQGEIVYVCRQLNKPVIVASQLLESMVEYPTPTRAEVAD 395

Query: 398 VSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRMELWSREENRENFLPHYQLGVSL 457
           VSEAVRQYADALMLS ESAIG YG KAL+VLQMAS RME WSREEN+++FL   QLGVSL
Sbjct: 396 VSEAVRQYADALMLSAESAIGLYGHKALAVLQMASSRMESWSREENQQSFLEQRQLGVSL 455

Query: 458 HDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALN 517
            DRIAE+ICNSA E+AN L+VDAIFV T  GHMASLLSRNRP PPIFA TDD+STRMALN
Sbjct: 456 PDRIAEQICNSAVEIANNLAVDAIFVFTNHGHMASLLSRNRPNPPIFAFTDDESTRMALN 515

Query: 518 LQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQL 577
           LQWGV PL ++L +D+EANI   I LVKSKGLVK+GDSVLVVS+++   +  +A QSIQ+
Sbjct: 516 LQWGVTPLLVDLLDDVEANIKVTISLVKSKGLVKEGDSVLVVSDVTTTISTPLAFQSIQV 575

Query: 578 KTI 581
           KTI
Sbjct: 576 KTI 578

BLAST of CmaCh16G003030 vs. TrEMBL
Match: A0A059AT14_EUCGR (Pyruvate kinase OS=Eucalyptus grandis GN=EUGRSUZ_I02501 PE=3 SV=1)

HSP 1 Score: 816.2 bits (2107), Expect = 2.5e-233
Identity = 407/565 (72.04%), Postives = 488/565 (86.37%), Query Frame = 1

Query: 17  AKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVDLEDAERLRRLEGLEAT 76
           +K P  D K    G+P+    ++C+  +   R+  GV+AV+QV +E A+ + ++EG    
Sbjct: 16  SKIPTFDFKSHISGVPM----IYCSGGKLKSRISTGVRAVLQVGVEAAQSVGKVEG---A 75

Query: 77  LGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREW 136
           LG DVV+E EL+EKGFLGMRKTKLVCTIGPACS++EDLE+LAL GMN+ARLNMCHNSR+W
Sbjct: 76  LGFDVVSEEELREKGFLGMRKTKLVCTIGPACSSLEDLERLALGGMNVARLNMCHNSRKW 135

Query: 137 HCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGYR 196
           H DVIRKIKKLNE KGFC+SVMIDTEGSQIHVVDHGAPSS+KVE+GS WLFT +KFEG R
Sbjct: 136 HLDVIRKIKKLNEEKGFCVSVMIDTEGSQIHVVDHGAPSSVKVEEGSFWLFTTKKFEGSR 195

Query: 197 SFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYSFWR 256
            FTVQA++ GFSEGI+VGD +VIDGGMA+FEVIEKIGNDL CKCTD GLFLP AK+SFWR
Sbjct: 196 PFTVQANHSGFSEGIEVGDELVIDGGMASFEVIEKIGNDLKCKCTDSGLFLPCAKFSFWR 255

Query: 257 DGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSAKSVR 316
           DG++V RN++ PTLS KDW+DI FGIAEGV+FIA+SFVN AD V++LK+Y+STKS+ S+R
Sbjct: 256 DGKLVERNYKLPTLSRKDWADINFGIAEGVDFIAMSFVNDADAVRNLKDYISTKSSISIR 315

Query: 317 VLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVII 376
           VLAKIESLESLQ L EI+EASDG+M+ARGDLGVEIPLEQIPA+QEEIT VCR++NKPVI+
Sbjct: 316 VLAKIESLESLQKLGEIVEASDGLMIARGDLGVEIPLEQIPAVQEEITYVCRQMNKPVIV 375

Query: 377 ASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRME 436
           ASQLLESMVE+PTPTRAEVADVSEAVRQYADALMLSGESAIGS+GQKALSVL+MAS RME
Sbjct: 376 ASQLLESMVEFPTPTRAEVADVSEAVRQYADALMLSGESAIGSHGQKALSVLRMASNRME 435

Query: 437 LWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLSR 496
            WSR+ENR++ L H +LGVSL DRIAE+IC+ A+E+AN L VDAIFV T  G MASLLSR
Sbjct: 436 TWSRQENRQSMLHHRKLGVSLPDRIAEQICDCAAEMANNLGVDAIFVYTKHGQMASLLSR 495

Query: 497 NRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDSV 556
           NRP  PIFA T+D STRMALNLQWGV PL ++L++D+EANI + I+L+K++GLV++GD+V
Sbjct: 496 NRPCSPIFAFTNDRSTRMALNLQWGVIPLFVDLTDDMEANIVKSIDLMKAEGLVREGDAV 555

Query: 557 LVVSEISPARAASMASQSIQLKTIA 582
           LVVS+I+P  A S A QSIQ+KTI+
Sbjct: 556 LVVSDITPTAARSTAFQSIQVKTIS 573

BLAST of CmaCh16G003030 vs. TAIR10
Match: AT3G22960.1 (AT3G22960.1 Pyruvate kinase family protein)

HSP 1 Score: 594.7 bits (1532), Expect = 6.0e-170
Identity = 292/487 (59.96%), Postives = 369/487 (75.77%), Query Frame = 1

Query: 79  LDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHC 138
           +D V E ELKE GF   R+TKL+CTIGPA    E LE LA+ GMN+ARLNMCH +R+WH 
Sbjct: 100 VDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHR 159

Query: 139 DVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGYR-S 198
            VIR +++LNE KGF +++M+DTEGS+IH+ D G  +S K EDG +W FT   F+  R  
Sbjct: 160 GVIRSVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPE 219

Query: 199 FTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYSFWRD 258
            T+  SY+GF+E ++VGD +++DGGM  FEVIEKIG D+ C CTDPGL LPRA  +FWRD
Sbjct: 220 RTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRD 279

Query: 259 GRVV-VRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKS-AKSV 318
           G +V  RN   PT+SSKDW DI+FGIAEGV+FIA+SFV  A+ + HLK+YL+ +S    +
Sbjct: 280 GSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEI 339

Query: 319 RVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVI 378
            V+AKIES++SL NLEEII ASDG MVARGDLG +IPLEQ+PA Q+ I  VCR LNKPVI
Sbjct: 340 GVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVI 399

Query: 379 IASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRM 438
           +ASQLLESM+EYPTPTRAEVADVSEAVRQ +DALMLSGESA+G +  KAL+VL+  S R+
Sbjct: 400 VASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRI 459

Query: 439 ELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLS 498
           E W REE R   +P   +G S  D+I+EEICNSA+++AN L VDA+FV TT GHMASL+S
Sbjct: 460 ERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVS 519

Query: 499 RNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDS 558
           R RP  PIFA T   S R  LNLQWG+ P R+  S+D+E+N+ +   L+KS+G++K GD 
Sbjct: 520 RCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDL 579

Query: 559 VLVVSEI 563
           V+ VS++
Sbjct: 580 VIAVSDM 586

BLAST of CmaCh16G003030 vs. TAIR10
Match: AT5G52920.1 (AT5G52920.1 plastidic pyruvate kinase beta subunit 1)

HSP 1 Score: 313.9 bits (803), Expect = 2.0e-85
Identity = 200/575 (34.78%), Postives = 310/575 (53.91%), Query Frame = 1

Query: 24  VKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVDLEDAERLRRLEGLEATLGLDVVA 83
           +K + F + V G     N  + SGRV    + V+   +  A    R+E     +  + V 
Sbjct: 30  LKPASFAVKVLG-----NEAKRSGRVSVRSRRVVDTTVRSA----RVETEVIPVSPEDVP 89

Query: 84  ERELKEKGFLGM-----------------RKTKLVCTIGPACSAIEDLEKLALEGMNIAR 143
            RE + +  L M                 RKTK+VCT+GP+ +  E + KLA  GMN+AR
Sbjct: 90  NREEQLERLLEMQQFGDTSVGMWSKPTVRRKTKIVCTVGPSTNTREMIWKLAEAGMNVAR 149

Query: 144 LNMCHNSREWHCDVIRKIKKLN-EGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIW 203
           +NM H     H  VI  +K+ N + K   I++M+DT+G ++   D   P  I ++ G  +
Sbjct: 150 MNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTKGPEVRSGD--LPQPIMLDPGQEF 209

Query: 204 LFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGL 263
            FT E+     S  V  +Y+ F   ++ GD +++DGGM +F V  K  + + C+  D G 
Sbjct: 210 TFTIERGVSTPS-CVSVNYDDFVNDVEAGDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGE 269

Query: 264 FLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKN 323
              R      R   V  ++   P+++ KDW DI+FG+   V+F A+SFV  A  V  LK 
Sbjct: 270 LKSR------RHLNVRGKSATLPSITEKDWEDIKFGVENKVDFYAVSFVKDAQVVHELKK 329

Query: 324 YLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITS 383
           YL    A  + V+ KIES +S+ NL  II ASDG MVARGDLG E+P+E++P +QEEI +
Sbjct: 330 YLQNSGA-DIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPILQEEIIN 389

Query: 384 VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKAL 443
           +CR + K VI+A+ +LESM+ +PTPTRAEV+D++ AVR+ ADA+MLSGE+A G +  KA 
Sbjct: 390 LCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAA 449

Query: 444 SVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLT 503
            V+   + R E           +P   LG +  + ++E     A+ ++N L    + V T
Sbjct: 450 GVMHTVALRTEATITSGE----MPP-NLGQAFKNHMSEMFAYHATMMSNTLGTSTV-VFT 509

Query: 504 TQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVK 563
             G MA LLS  RP   I+A T++   +  L L  GV P+ +E ++D E      +  + 
Sbjct: 510 RTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGVCPIYMEFTDDAEETFANALATLL 569

Query: 564 SKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 581
            +G+VK+G+ + +V   +     S ++ +IQ++ +
Sbjct: 570 KQGMVKKGEEIAIVQSGTQPIWRSQSTHNIQVRKV 579

BLAST of CmaCh16G003030 vs. TAIR10
Match: AT1G32440.1 (AT1G32440.1 plastidial pyruvate kinase 3)

HSP 1 Score: 310.5 bits (794), Expect = 2.3e-84
Identity = 190/486 (39.09%), Postives = 279/486 (57.41%), Query Frame = 1

Query: 96  RKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHCDVIRKIKKLNE-GKGFC 155
           RKTK+VCTIGP+ S+ E + KLA  GMN+ARLNM H     H   I  +K+ N       
Sbjct: 99  RKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLVKEYNSLFVDKA 158

Query: 156 ISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVG 215
           I++M+DT+G ++   D   P  I +E+G  + FT ++    +  TV  +Y+ F   ++VG
Sbjct: 159 IAIMLDTKGPEVRSGD--VPQPIFLEEGQEFNFTIKRGVSLKD-TVSVNYDDFVNDVEVG 218

Query: 216 DSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKD 275
           D +++DGGM +  V  K  + + C   D G    R      R   V  ++   P+++ KD
Sbjct: 219 DILLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSR------RHLNVRGKSATLPSITDKD 278

Query: 276 WSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSAKSVRVLAKIESLESLQNLEEII 335
           W DI+FG+   V+F A+SFV  A  V  LKNYL T SA  + V+ KIES +S++NL  II
Sbjct: 279 WEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYLKTCSA-DISVIVKIESADSIKNLPSII 338

Query: 336 EASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVIIASQLLESMVEYPTPTRAE 395
            A DG MVARGDLG E+P+E++P +QEEI   CR ++KPVI+A+ +LESM+ +PTPTRAE
Sbjct: 339 SACDGAMVARGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNMLESMINHPTPTRAE 398

Query: 396 VADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRMELWSREENRENFLPHYQLG 455
           V+D++ AVR+ ADA+MLSGE+A G +  KA++V+   + R E         +    Y+  
Sbjct: 399 VSDIAIAVREGADAIMLSGETAHGKFPLKAVNVMHTVALRTEASLPVRTSASRTTAYK-- 458

Query: 456 VSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRM 515
                 + +     AS +AN LS   + V T  G MA LLS  RP   IFA T+      
Sbjct: 459 ----GHMGQMFAFHASIMANTLS-SPLIVFTRTGSMAVLLSHYRPSATIFAFTNQRRIMQ 518

Query: 516 ALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDSVLVVSEISPARAASMASQS 575
            L L  GV P+ +E S+D E    R ++L++ + ++K+G  V +V   S       ++  
Sbjct: 519 RLALYQGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPIWREESTHL 567

Query: 576 IQLKTI 581
           IQ++ I
Sbjct: 579 IQVRKI 567

BLAST of CmaCh16G003030 vs. TAIR10
Match: AT5G63680.1 (AT5G63680.1 Pyruvate kinase family protein)

HSP 1 Score: 225.7 bits (574), Expect = 7.3e-59
Identity = 158/494 (31.98%), Postives = 254/494 (51.42%), Query Frame = 1

Query: 97  KTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCIS 156
           KTK+VCT+GPA  ++  +EKL   GMN+AR N  H S E+H + +  ++   +  G   +
Sbjct: 21  KTKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSHEYHQETLDNLRTAMQNTGILAA 80

Query: 157 VMIDTEGSQIHV--VDHGAPSSIKVEDGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVG 216
           VM+DT+G +I    +  G P  +K E   I + T    +G    T+  SY+     ++ G
Sbjct: 81  VMLDTKGPEIRTGFLKDGNPIQLK-EGQEITITTDYDIKGDEK-TISMSYKKLPVDVKPG 140

Query: 217 DSVVIDGGMATFEVIEKIGN--DLSCKCTDPGLFLPRAKYSFWRDGRVVVRNHESPTLSS 276
           ++++   G  +  V+    N   + C+C +  +   R   +    G VV    + PTL+ 
Sbjct: 141 NTILCADGSISLAVVSCDPNAGTVICRCENTAMLGERKNVNL--PGVVV----DLPTLTD 200

Query: 277 KDWSDI-EFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSAKSVRVLAKIESLESLQNLE 336
           KD  DI ++G+   ++ IALSFV     + +++  L + S KS+ +++K+E+ E + N +
Sbjct: 201 KDVEDILKWGVPNNIDMIALSFVRKGSDLVNVRKVLGSHS-KSIMLMSKVENQEGVLNFD 260

Query: 337 EIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVIIASQLLESMVEYPTPT 396
           EI+  +D  MVARGDLG+EIP+E+I   Q+ +   C    KPV+ A+Q+LESM++ P PT
Sbjct: 261 EILRETDAFMVARGDLGMEIPIEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPT 320

Query: 397 RAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRMELWSREENRENFLPHY 456
           RAE  DV+ AV    D +MLSGESA G+Y +  ++V  MA   +E  S  +    F    
Sbjct: 321 RAEATDVANAVLDGTDCVMLSGESAAGAYPE--IAVKTMAKICIEAESSLDYNTIFKEMI 380

Query: 457 QLGVSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLSRNRPRPPIFAL----- 516
           +    L     E + +SA   AN+     I VLT  G  A L+++ RP  PI ++     
Sbjct: 381 R-ATPLPMSTLESLASSAVRTANKAKAKLIIVLTRGGTTAKLVAKYRPAVPILSVVVPVF 440

Query: 517 ---------TDDDSTRMALNLQWGVFPL------RIELSEDIEANIWRGIELVKSKGLVK 566
                    +D+   R +L +  G+ P+      +   SE  E  I   ++    KGL  
Sbjct: 441 TSDTFNWSCSDESPARHSL-IYRGLIPVLGEGSAKATDSESTEEIIESALKSATEKGLCN 500

BLAST of CmaCh16G003030 vs. TAIR10
Match: AT5G08570.1 (AT5G08570.1 Pyruvate kinase family protein)

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-58
Identity = 158/494 (31.98%), Postives = 254/494 (51.42%), Query Frame = 1

Query: 97  KTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCIS 156
           KTK+VCT+GPA   +  +EKL   GMN+AR N  H S E+H + +  ++      G   +
Sbjct: 21  KTKIVCTLGPASRTVSMIEKLLKAGMNVARFNFSHGSHEYHQETLDNLRSAMHNTGILAA 80

Query: 157 VMIDTEGSQIHV--VDHGAPSSIKVEDGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVG 216
           VM+DT+G +I    +  G P  +K E   I + T    +G  S T+  SY+     ++ G
Sbjct: 81  VMLDTKGPEIRTGFLKDGNPIQLK-EGQEITITTDYDIQGDES-TISMSYKKLPLDVKPG 140

Query: 217 DSVVIDGGMATFEVI--EKIGNDLSCKCTDPGLFLPRAKYSFWRDGRVVVRNHESPTLSS 276
           ++++   G  +  V+  +     + C+C +  +   R   +    G VV    + PTL+ 
Sbjct: 141 NTILCADGSISLAVLSCDPESGTVRCRCENSAMLGERKNVNL--PGVVV----DLPTLTD 200

Query: 277 KDWSDI-EFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSAKSVRVLAKIESLESLQNLE 336
           KD  DI  +G+   ++ IALSFV     + +++  L +  AKS+ +++K+E+ E + N +
Sbjct: 201 KDIEDILGWGVPNSIDMIALSFVRKGSDLVNVRKVLGSH-AKSIMLMSKVENQEGVINFD 260

Query: 337 EIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVIIASQLLESMVEYPTPT 396
           EI+  +D  MVARGDLG+EIP+E+I   Q+ +   C    KPV+ A+Q+LESM++ P PT
Sbjct: 261 EILRETDAFMVARGDLGMEIPIEKIFLAQKLMIYKCNLAGKPVVTATQMLESMIKSPRPT 320

Query: 397 RAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRMELWSREENRENFLPHY 456
           RAE  DV+ AV    D +MLSGESA G+Y + A+ V  MA   +E  S  +    F    
Sbjct: 321 RAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVKV--MAKICIEAESSLDYNTIFKEMI 380

Query: 457 QLGVSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLSRNRPRPPIFAL----- 516
           +    L     E + +SA   AN+     I VLT  G  A+L+++ RP  PI ++     
Sbjct: 381 R-ATPLPMSPLESLASSAVRTANKARAKLIIVLTRGGSTANLVAKYRPAVPILSVVVPVM 440

Query: 517 ---------TDDDSTRMALNLQWGVFPLRIE------LSEDIEANIWRGIELVKSKGLVK 566
                    +D+   R +L +  G+ P+  E       SE  E  I   ++    +GL  
Sbjct: 441 TTDSFDWSCSDESPARHSL-IYRGLIPMLAEGSAKATDSEATEVIIEAALKSATQRGLCN 500

BLAST of CmaCh16G003030 vs. NCBI nr
Match: gi|659108859|ref|XP_008454424.1| (PREDICTED: plastidial pyruvate kinase 1, chloroplastic-like [Cucumis melo])

HSP 1 Score: 1043.9 bits (2698), Expect = 1.1e-301
Identity = 529/581 (91.05%), Postives = 553/581 (95.18%), Query Frame = 1

Query: 1   MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFG-RTVWCNRRRTSGRVGNGVKAVMQV 60
           MAEVNAMMIRA CNF+ + PVLDVKR GF +PV G RTVWCNRRR  GRVGNGVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120
           DL+D ERLRRLEGLEATLGLD+VAERELKEKGFLGMRKTKLVCTIGPACS IEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKV 180
           EGMNIARLNMCHNSREWHCD+I+KIKKLNE KGFCISVMID EGS+IHVVDHGAPSS+KV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCK 240
           EDGSIWLFTAEKFEG R FTVQASYEGFSEGI+VGDS+VIDGGMATFEV EKIGNDLSC+
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADP 300
           CTDPGLFLPRAK SFWRDGR+VVRNHESPTLSSKDWSDIEFGI+EGV+FIALSFVN ADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VKHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAI 360
           V+HLKNYL TKSAKS+RVLAKIESLE+LQNLEEIIEASDGIMVARGDLGVEIPLEQIPA+
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVD 480
           YGQKALSVLQMAS RMELWSREEN +NFLP +QLGVSLHDRIAEEICNSA+ELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWR 540
           AIFVLT QGHMASLLSRNRP PPIFALTDDDSTRMALNLQWGVFPLRI+LSEDIEANI R
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 581
           GIE+VKSKGLVKQGDSVLVVSEISPARA SMASQSIQLKTI
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTI 581

BLAST of CmaCh16G003030 vs. NCBI nr
Match: gi|449465101|ref|XP_004150267.1| (PREDICTED: plastidial pyruvate kinase 1, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 1039.6 bits (2687), Expect = 2.0e-300
Identity = 526/581 (90.53%), Postives = 553/581 (95.18%), Query Frame = 1

Query: 1   MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFGR-TVWCNRRRTSGRVGNGVKAVMQV 60
           MAEVNAMMIRA CNF+ + PVLDVKR GF +PV GR TVWC+RRR  GRV NGVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVVNGVKATMQV 60

Query: 61  DLEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120
           DLED ERL+RLEGLEATLGLD+VAERELKEKGFLGMRKTKLVCT+GPACS I DLEKLAL
Sbjct: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKV 180
           EGMNIARLNMCHNS EWHCD+I+KIKKLNE KGFCISVMID EGSQIHVVDHGAPSS+KV
Sbjct: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCK 240
           EDGSIWLFTA+KFEG R FTVQASYEGFSEGI+VGDS+VIDGGMATFEVIEKIGNDLSC+
Sbjct: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADP 300
           CTDPGLFLPRAK SFWRDGR+V+RNHESPTLSSKDWSDIEFGI+EGV+FIALSFVN ADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VKHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAI 360
           V+HLKNYLSTKSAKS+RVLAKIESLE+LQNLEEI+EASDGIMVARGDLGVEIPLEQIPA+
Sbjct: 301 VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVD 480
           YGQKALSVLQMAS RMELWSREENR NFLP +QLGVSLHDRIAEEICNSA+ELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWR 540
           AIFVLT QGHMASLLSRNRP PPIFALTDDDSTRMALNLQWGVFPLRI+LSEDIEANI R
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 581
           GIE+VKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 581

BLAST of CmaCh16G003030 vs. NCBI nr
Match: gi|645261341|ref|XP_008236247.1| (PREDICTED: pyruvate kinase isozyme A, chloroplastic-like isoform X3 [Prunus mume])

HSP 1 Score: 850.1 bits (2195), Expect = 2.2e-243
Identity = 428/565 (75.75%), Postives = 494/565 (87.43%), Query Frame = 1

Query: 17  AKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVDLEDAERLRRLEGLEAT 76
           AK P +++++  FG+PV    V C  ++  G+VG GV+AV QV LE++ER+R L+ LE T
Sbjct: 19  AKNPRVNLEKQVFGVPVLHHGVSCIGKKLKGKVGAGVQAV-QVGLEESERVRNLKSLERT 78

Query: 77  LGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREW 136
           L LD V+EREL+EKGFLG+RKTKLVCTIGPACS++EDLEKL L GMN+ARLNMCHN+REW
Sbjct: 79  LELDAVSERELREKGFLGLRKTKLVCTIGPACSSLEDLEKLVLGGMNVARLNMCHNTREW 138

Query: 137 HCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGYR 196
           H DVIRKIKKLNE KGF +SVMIDTEGSQIHVVDHGAPSS+KV++ SIWLFT EKF+G  
Sbjct: 139 HRDVIRKIKKLNEEKGFSVSVMIDTEGSQIHVVDHGAPSSLKVQEDSIWLFTNEKFDGSV 198

Query: 197 SFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYSFWR 256
            FTVQA+YEGFSEGI+VGD +VIDGGMA FEVIEK+G+DL CKCTDPGLFLPRAK+SFWR
Sbjct: 199 PFTVQANYEGFSEGIKVGDELVIDGGMARFEVIEKLGSDLRCKCTDPGLFLPRAKFSFWR 258

Query: 257 DGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSAKSVR 316
           DG++V RN+E PTLS+KDWSDIEFGI+EGVNFIA+SFVN A+ V+ LKNYLSTKSA SVR
Sbjct: 259 DGKLVERNYELPTLSTKDWSDIEFGISEGVNFIAMSFVNDAESVRQLKNYLSTKSAASVR 318

Query: 317 VLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVII 376
           VLAKIESLESLQ LEEIIEASDGIMVAR DLGVEIPLEQIP +QEEIT VCR+LNKPVI+
Sbjct: 319 VLAKIESLESLQKLEEIIEASDGIMVARVDLGVEIPLEQIPTVQEEITKVCRQLNKPVIV 378

Query: 377 ASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRME 436
           ASQ LESM+EYPTPTRAEVAD+SEAVRQYADALML+GESAIG +GQKALSVLQM S R+E
Sbjct: 379 ASQFLESMIEYPTPTRAEVADISEAVRQYADALMLAGESAIGLFGQKALSVLQMTSSRLE 438

Query: 437 LWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLSR 496
            WSREENR+  L   QLGVSL DRIAE+ICNSA ELAN L+VDAIFV T  GHMASLLSR
Sbjct: 439 SWSREENRQGSLLPRQLGVSLPDRIAEQICNSAVELANNLAVDAIFVYTKHGHMASLLSR 498

Query: 497 NRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDSV 556
           NRP PPIFA T+D+STRM+LNLQWGV PL ++LS+D+E+NI   I L+KSKGLVK+GDSV
Sbjct: 499 NRPNPPIFAFTNDESTRMSLNLQWGVIPLLVDLSDDMESNISSTIHLIKSKGLVKEGDSV 558

Query: 557 LVVSEISPARAASMASQSIQLKTIA 582
           LVVS+++P+RA  MA QSIQ+KTIA
Sbjct: 559 LVVSDVTPSRATPMAFQSIQVKTIA 582

BLAST of CmaCh16G003030 vs. NCBI nr
Match: gi|595796631|ref|XP_007201178.1| (hypothetical protein PRUPE_ppa003382mg [Prunus persica])

HSP 1 Score: 843.6 bits (2178), Expect = 2.1e-241
Identity = 426/565 (75.40%), Postives = 491/565 (86.90%), Query Frame = 1

Query: 17  AKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVDLEDAERLRRLEGLEAT 76
           +K P +++++  FG+PV    V C  ++  G+VG GV+AV QV LE++ER+R L  LE T
Sbjct: 16  SKNPRVNLEKQVFGVPVLHHGVSCIGKKLKGKVGAGVQAV-QVGLEESERVRNLRSLERT 75

Query: 77  LGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREW 136
           L LD V+EREL+EKGFLG+RKTKLVCTIGPACS++EDLEKL L GMN+ARLNMCHN+REW
Sbjct: 76  LELDAVSERELREKGFLGLRKTKLVCTIGPACSSLEDLEKLVLGGMNVARLNMCHNTREW 135

Query: 137 HCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGYR 196
           H DVIRKIKKLNE KGF +SVMIDTEGSQIHVVDHGAPSS+KV++ SIWLFT EKF+G  
Sbjct: 136 HRDVIRKIKKLNEEKGFSVSVMIDTEGSQIHVVDHGAPSSLKVQEDSIWLFTNEKFDGSV 195

Query: 197 SFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYSFWR 256
            FTVQA YEGFSEGI+VGD +VIDGGMA FEVIEK+ +DL CKCTDPGLFLPRAK+SFWR
Sbjct: 196 PFTVQAKYEGFSEGIKVGDELVIDGGMARFEVIEKLRSDLRCKCTDPGLFLPRAKFSFWR 255

Query: 257 DGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSAKSVR 316
           DG++V RN+E PTLS+KDWSDIEFGI+EGVNFIA+SFVN A+ V+HLKNYLSTKSA S+R
Sbjct: 256 DGKLVERNYELPTLSTKDWSDIEFGISEGVNFIAMSFVNDAESVRHLKNYLSTKSATSIR 315

Query: 317 VLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVII 376
           VLAKIESLESLQ LEEIIEASDGIMVAR DLGVEIPLEQIP +QEEIT VCR+LNKPVI+
Sbjct: 316 VLAKIESLESLQKLEEIIEASDGIMVARVDLGVEIPLEQIPTVQEEITKVCRQLNKPVIV 375

Query: 377 ASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRME 436
           ASQ LESM+EYPTPTRAEVAD+SEAVRQYADALML+GESAIG +GQKALSVLQM S RME
Sbjct: 376 ASQFLESMIEYPTPTRAEVADISEAVRQYADALMLAGESAIGLFGQKALSVLQMTSSRME 435

Query: 437 LWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLSR 496
             SREENR+  L   QLGVSL DRIAE+ICNSA ELAN L+VDAIFV T  GHMASLLSR
Sbjct: 436 SSSREENRQGSLLPRQLGVSLPDRIAEQICNSAVELANNLAVDAIFVYTKHGHMASLLSR 495

Query: 497 NRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDSV 556
           NRP PPIFA T+D+STRM+LNLQWGV PL ++LS+D+E+NI   I L+KSKGLVK+GDSV
Sbjct: 496 NRPNPPIFAFTNDESTRMSLNLQWGVIPLLVDLSDDMESNISSTIHLIKSKGLVKEGDSV 555

Query: 557 LVVSEISPARAASMASQSIQLKTIA 582
           LVVS+++P+RA  MA QSIQ+KTIA
Sbjct: 556 LVVSDVTPSRATPMAFQSIQVKTIA 579

BLAST of CmaCh16G003030 vs. NCBI nr
Match: gi|645261339|ref|XP_008236246.1| (PREDICTED: pyruvate kinase isozyme A, chloroplastic-like isoform X2 [Prunus mume])

HSP 1 Score: 843.6 bits (2178), Expect = 2.1e-241
Identity = 424/561 (75.58%), Postives = 490/561 (87.34%), Query Frame = 1

Query: 17  AKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVDLEDAERLRRLEGLEAT 76
           AK P +++++  FG+PV    V C  ++  G+VG GV+AV QV LE++ER+R L+ LE T
Sbjct: 19  AKNPRVNLEKQVFGVPVLHHGVSCIGKKLKGKVGAGVQAV-QVGLEESERVRNLKSLERT 78

Query: 77  LGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREW 136
           L LD V+EREL+EKGFLG+RKTKLVCTIGPACS++EDLEKL L GMN+ARLNMCHN+REW
Sbjct: 79  LELDAVSERELREKGFLGLRKTKLVCTIGPACSSLEDLEKLVLGGMNVARLNMCHNTREW 138

Query: 137 HCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGYR 196
           H DVIRKIKKLNE KGF +SVMIDTEGSQIHVVDHGAPSS+KV++ SIWLFT EKF+G  
Sbjct: 139 HRDVIRKIKKLNEEKGFSVSVMIDTEGSQIHVVDHGAPSSLKVQEDSIWLFTNEKFDGSV 198

Query: 197 SFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYSFWR 256
            FTVQA+YEGFSEGI+VGD +VIDGGMA FEVIEK+G+DL CKCTDPGLFLPRAK+SFWR
Sbjct: 199 PFTVQANYEGFSEGIKVGDELVIDGGMARFEVIEKLGSDLRCKCTDPGLFLPRAKFSFWR 258

Query: 257 DGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSAKSVR 316
           DG++V RN+E PTLS+KDWSDIEFGI+EGVNFIA+SFVN A+ V+ LKNYLSTKSA SVR
Sbjct: 259 DGKLVERNYELPTLSTKDWSDIEFGISEGVNFIAMSFVNDAESVRQLKNYLSTKSAASVR 318

Query: 317 VLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVII 376
           VLAKIESLESLQ LEEIIEASDGIMVAR DLGVEIPLEQIP +QEEIT VCR+LNKPVI+
Sbjct: 319 VLAKIESLESLQKLEEIIEASDGIMVARVDLGVEIPLEQIPTVQEEITKVCRQLNKPVIV 378

Query: 377 ASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRME 436
           ASQ LESM+EYPTPTRAEVAD+SEAVRQYADALML+GESAIG +GQKALSVLQM S R+E
Sbjct: 379 ASQFLESMIEYPTPTRAEVADISEAVRQYADALMLAGESAIGLFGQKALSVLQMTSSRLE 438

Query: 437 LWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLSR 496
            WSREENR+  L   QLGVSL DRIAE+ICNSA ELAN L+VDAIFV T  GHMASLLSR
Sbjct: 439 SWSREENRQGSLLPRQLGVSLPDRIAEQICNSAVELANNLAVDAIFVYTKHGHMASLLSR 498

Query: 497 NRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDSV 556
           NRP PPIFA T+D+STRM+LNLQWGV PL ++LS+D+E+NI   I L+KSKGLVK+GDSV
Sbjct: 499 NRPNPPIFAFTNDESTRMSLNLQWGVIPLLVDLSDDMESNISSTIHLIKSKGLVKEGDSV 558

Query: 557 LVVSEISPARAASMASQSIQL 578
           LVVS+++P+RA  MA QSIQ+
Sbjct: 559 LVVSDVTPSRATPMAFQSIQV 578

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PKP1_ARATH1.1e-16859.96Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana GN=PKP1 PE=1... [more]
KPYA_RICCO2.6e-16758.45Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis PE=1 SV=1[more]
KPYA_TOBAC3.0e-16357.43Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum PE=2 SV=1[more]
KPYG_TOBAC8.6e-8640.22Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum PE=2 SV=1[more]
PKP2_ARATH3.6e-8434.78Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana GN=PKP2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
E5GBH7_CUCME7.4e-30291.05Pyruvate kinase OS=Cucumis melo subsp. melo PE=3 SV=1[more]
A0A0A0KSY4_CUCSA1.4e-30090.53Pyruvate kinase OS=Cucumis sativus GN=Csa_4G000560 PE=3 SV=1[more]
M5VME8_PRUPE1.5e-24175.40Pyruvate kinase OS=Prunus persica GN=PRUPE_ppa003382mg PE=3 SV=1[more]
W9R7Y8_9ROSA3.9e-23474.59Pyruvate kinase OS=Morus notabilis GN=L484_003357 PE=3 SV=1[more]
A0A059AT14_EUCGR2.5e-23372.04Pyruvate kinase OS=Eucalyptus grandis GN=EUGRSUZ_I02501 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G22960.16.0e-17059.96 Pyruvate kinase family protein[more]
AT5G52920.12.0e-8534.78 plastidic pyruvate kinase beta subunit 1[more]
AT1G32440.12.3e-8439.09 plastidial pyruvate kinase 3[more]
AT5G63680.17.3e-5931.98 Pyruvate kinase family protein[more]
AT5G08570.11.2e-5831.98 Pyruvate kinase family protein[more]
Match NameE-valueIdentityDescription
gi|659108859|ref|XP_008454424.1|1.1e-30191.05PREDICTED: plastidial pyruvate kinase 1, chloroplastic-like [Cucumis melo][more]
gi|449465101|ref|XP_004150267.1|2.0e-30090.53PREDICTED: plastidial pyruvate kinase 1, chloroplastic-like [Cucumis sativus][more]
gi|645261341|ref|XP_008236247.1|2.2e-24375.75PREDICTED: pyruvate kinase isozyme A, chloroplastic-like isoform X3 [Prunus mume... [more]
gi|595796631|ref|XP_007201178.1|2.1e-24175.40hypothetical protein PRUPE_ppa003382mg [Prunus persica][more]
gi|645261339|ref|XP_008236246.1|2.1e-24175.58PREDICTED: pyruvate kinase isozyme A, chloroplastic-like isoform X2 [Prunus mume... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001697Pyr_Knase
IPR011037Pyrv_Knase-like_insert_dom_sf
IPR015793Pyrv_Knase_brl
IPR015794Pyruvate kinase, alpha/beta
IPR015795Pyrv_Knase_C
IPR015806Pyrv_Knase_insert_dom_sf
IPR015813Pyrv/PenolPyrv_Kinase-like_dom
IPR018209Pyrv_Knase_AS
Vocabulary: Molecular Function
TermDefinition
GO:0000287magnesium ion binding
GO:0004743pyruvate kinase activity
GO:0030955potassium ion binding
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0006096glycolytic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015976 carbon utilization
biological_process GO:0006094 gluconeogenesis
biological_process GO:0006096 glycolytic process
biological_process GO:0006144 purine nucleobase metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016301 kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G003030.1CmaCh16G003030.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 317..343
score: 7.2E-51coord: 369..393
score: 7.2E-51coord: 413..429
score: 7.2E-51coord: 344..368
score: 7.2E-51coord: 153..169
score: 7.2E-51coord: 394..412
score: 7.2E-51coord: 286..300
score: 7.2
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 53..580
score:
IPR001697Pyruvate kinaseTIGRFAMsTIGR01064TIGR01064coord: 96..567
score: 6.9E
IPR011037Pyruvate kinase-like, insert domainunknownSSF50800PK beta-barrel domain-likecoord: 166..250
score: 4.7
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 95..433
score: 2.6
IPR015794Pyruvate kinase, alpha/betaGENE3DG3DSA:3.40.1380.20coord: 424..567
score: 3.2
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 463..563
score: 5.2
IPR015795Pyruvate kinase, C-terminalunknownSSF52935PK C-terminal domain-likecoord: 459..562
score: 4.45
IPR015806Pyruvate kinase, beta-barrel insert domainGENE3DG3DSA:2.40.33.10coord: 200..244
score: 1.
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domainGENE3DG3DSA:3.20.20.60coord: 268..423
score: 4.2E-84coord: 96..168
score: 4.2
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domainunknownSSF51621Phosphoenolpyruvate/pyruvate domaincoord: 90..169
score: 1.51E-76coord: 267..436
score: 1.51
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 315..327
scor
NoneNo IPR availablePANTHERPTHR11817:SF21SUBFAMILY NOT NAMEDcoord: 53..580
score:

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh16G003030CmaCh04G004280Cucurbita maxima (Rimu)cmacmaB351
The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh16G003030Cucumber (Chinese Long) v3cmacucB0414
CmaCh16G003030Cucurbita maxima (Rimu)cmacmaB135
CmaCh16G003030Cucumber (Gy14) v1cgycmaB0623
CmaCh16G003030Cucurbita moschata (Rifu)cmacmoB318
CmaCh16G003030Cucumber (Chinese Long) v2cmacuB348
CmaCh16G003030Cucumber (Gy14) v2cgybcmaB494
CmaCh16G003030Melon (DHL92) v3.6.1cmamedB331