Carg08103 (gene) Silver-seed gourd

Overview
NameCarg08103
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionPyruvate kinase
LocationCucurbita_argyrosperma_scaffold_050 : 736863 .. 741733 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCAGGTTTTCGTCAATGGCGGAGGTGAGTGCTATGATGATTCGTGCTTGTTGCAATTTCGTTGCTAAAACCTCTGTGTTAGATGTGAAACGATCGGATTCTCGTTTGTCGGTTATCGGTCGAACAGTTTGGTGCGATCGGGGTAGGATGAAGGGGAGCGTTGTAACTGGAGTTAAGGCGGCAATGCAGGTGGATTTGGAGGATGATGAGAGGTTGAAACGATTGGACGGTTTAGAGGCGACATTAGGGCTTGATGTTGTGGCGGAGAGGGAATTGAAGGAGAAAGGGTTTTTGGGGATGAGGAAGACGAAGTTTGTTTGTACGATTGGGCCTGCGTGTTGTGCGATTGAGGATTTGGAGAAGTTGGCTTTGGAAGGTATGAACATTGCGAGATTGAATATGTGTCACAACAAGAAGGAGTGGCACTATGACGTGATTAGGAAGATCAAGAAGTTGAATGAAAAAGGATTTTGTATTTCGGTGATGATTGATACTGAAGGAAGTCAGATTCATGTTGTTGATCATGGAGCTCCTTCCTCCGTCAAAGTAGAGGTAGATTTTATATCTCTGTTATTTTTTCTGTAATTTTAGTGGTAATCAAATCAACAGAGAGTTATGATCCAGTGTTACTATTTTCCATTGTAATTTAAGAAAAACGGGGTTTAAATTCTCTGGCCATCATTGTTCAGGATGGTTCAAATTGGTTGTTTACTGCTGAGAAATTCGAGGGCTCTCGTCCATTTACTGTTCAAGCGAGCTATGAAGGCTTTTCTGAAGGTGCTGCACTTTGAATCTTTTTTTTTGTTTTCTCTGTCTTCTGAATTGTGATTAGAAGAAATGTATGAATCCCACAAGTTAAATAGGATATGAGTTTGAATTCTACAGGTATCCAAGTGGGTGATTCAATTGTGATTGATGGGGGAATGGCATCCTTTGAAGTTATGGAAAAGATTGGGAATGATTTAAGCTGTAAGTGCACAGACCCTGGTTTGTTTCTTCCTCGAGCAAAATGCAGCTTTTGGAGGGATGGGAGGCTTGTTATGAGAAAACATGAGTCTCCCACGCTGTCAAGTAAGGTAGGAAAGTGTCTGATGCATATCAATATTCATTCTGAATCATTTACTTGTGATGAAGCAGGTATTGTACGTTTGGAATTTCCCTTTCGAGCTCTCCTCAAAATTTTTAAAACGTGTCAGTTAGGAAGAGGGTTCCACACCCTTATAAAGAATGCTTCTCCTCTCCGACATGGAATCTCACAATCCACCCTCTTTGGGGCCAGCGTCTTCGCTGGCACTCGTTCCCCTCTCCAATGGACGTGGGATCTTTGAACCTCACAAGTTTTTACCCCTCCCCAACGGTGTGGACATGATGTATAAGGTTCACCGTTGAAAATTTTTCTTACTCTTGGGTTGTCAACAGGACAAGGTTTTACTGAATATGGTATGGCGACAAAAATGTTATGTTCATCATTAAAAATTTGTCTTCTCATAGTGGTTGTCAACATTATAAGATTTTGTTTCTGATTGGTATTGTGGATGACATTTTAGGATCATTGTTTAAATTTATTTTCTTGGGTTGTCAGCATCACAAGTTTTATCCCTGTATATCAACATCTATGTGCAGAAGAAATTTTATCCACTTTTCTGCAATTTTGCTCTCTTTTGACAAGGTTGACGATCGCATTAATGTGTCAAAATGTAAAAAATAATAACAAAAAAAAAAAAAGAGAGAGAGAAGAATTTAATGAATTACGTCTTCTTCTTGGATGATGCTAGTTCTCTGCTTTTCAACTCTGTTTTTCACAGTGTATTATACTATTTGGTTTACTATTTTATAGGATTGGTCCGACATTGAATTTGGAATCTCGGAAGGCGTCGACTTCATTGCCCTTTCCTTTGTGAACAGTGCAGATTCTGTCAAGCATCTAAAGAGCTACCTATCAGCAAAATCAGCAAAGTAAGCATCGATTCAAGAAGTCAACATATTAGCATGAGTTAGGTTGGAATATCACTACTAGGCATGAACGATATATCCCGATGTCCCTTTCAATTGAATTTTCATCACCACTGGTTTCTTAGCCGCAATTCATAAATTGTCTGGAAATCTTCTTTATTGAAACTGATATTGTGTAATTTCAAAACCCATTTTAGTAGTTAAGGATAAAAAAAATGTTCTCATGCACAGATCTGTGAGAGTATTGGCCAAGATTGAAAGCTTGGAGTCCCTTCAAAATCTAGAAGAAATCATAAAGGCTTCTGATGGAATCATGGTGGCTCGAGGGGATCTTGGGGTTGAAATTCCTCTTGAGCAGATACCAGCAGTCCAAGAAGAGATAACTAGTGTCTGTAGGGAGCTGAACAAACCAGTGATCGTAGCTTCTCAACTTCTAGAGTCGATGGTTGAATATCCTACTCCAACACGAGCTGAGGTACGAGTTTTTTAGTTAGTTAGTCTTTTTCAACTTCAGGTACAGGGATCCAACTATAATTCTCGTGAGATCCCACATCGGTTGGAGATGAGAACGAAACATTTCTTATAAAGGTGTGGAAACTTCTTCCTAGCAGATGCGTTTTAAAACCGTTAGACTGATAGTGATACGTAATGGGCCAAAGTAGACAATATCTACTAGTGGTGGGCTTAAGATGTTATAAATGGTATAGAGCCAAAATCGGGCACTGTGCCAGTGAGGACGTTGGGCCCTCAAGGGGAGTGGATTGTGAGACCTCACATCGGTTGGAAAAGGGAACGAAACATTCTTTATAAAGAGGGTAGAAACCTTTCCCTAACAGACATGTTTTAAAATTGTGAGGTTGAGACGATAGGGCCAAAACGGACAGTTATTCAAAAACATATTTCCGAGTTGAAATTTCTGGTGCTCTCTGGTATGGTGGATTGAATATTGTGTTGAAGTCTAGAATGATGATCAGTCTTCGTGTTCAATAGGTTGCAGATGTTTCTGAAGTTGTCCGACAGTATGCCGATGCATTGATGCTGTCTGGAGAGTCTGCCATAGGAGCATATGGACAGAAAGCGCTTTCGGTTCTGCAGATAGCCAGCAGCCGAATGGAGTTATGGAGTCGTGAAGAAAATACGAAACATTTCCTTCCCCAACACCAACTTGGGGTGTCATTGCACGATCGCATTGCCGAAGAAATTTGCAATTCTGCAGCTGAATTGGGTAAAACTCACTTTTCTCCACTTGCTTTCTTAACAATCCCTTCGTGTGTGATCATGAAAATAACATCAGAATCCCCTTTCGGTCATATATCATGTCTAACTTCTAGCTTCTTTATTACGTAATCATGCATAAGAAAATTATAAAGTTATTCTTTGTTAGTTTCAATTTGGTACTTTTTGTTTAGAAGTACATATTAACAAAATCTTTCATATCATCTTGGCTGTTCGCTTCTCAAAAAATTAAGTTTCGTTTGATGGGAGAGATAAACCATGGTAGATTGACTTAGTAGTCAATGTAAACGATAAAAGACTCGGACGAAATGAGTTCAAGACATGATGGTTATTTACCTAAGATTTAATATGTGTGACAGCCCAAGTCCACCGTTAGCAACTATTGTATGCTTTTGTCCCTTCACGATTTTAAAACGCGTCTGCTAGGGAGAAGTTTCTACACCCTTATAAAGAAAGTTTTGTTCTCCTCTCCAACCAATGTGGGCTCAGCATTCGCACTGTCATACCATTCGGTGTTTGACTCTGATACCATTTATAACAATCTAAGCCCACCGCTAACCGACATTGTCAATTTTATGTATTGTCGTCAGCCTCACAATTCTAAAACGCATCTATCAGAGAGAGATTTCCACACTCTCATAAATAATGTTTTGTTCCCCTCTTCAGCTAGGATAGGATCTATGAATTATCTTGAAAACCATATCTAATAGGGTTAGACAATTCTCTATGCCAACAGGGTTCGCTAGTTTAGACACTCACAGATATAAAAGTACAAATGGCTAGTGGTACTGAATTGGAGTAAAAAGAAAGTGTCGTATACAAAAGTAGATAACATTTAGCAACAACAGAGACATATTGTATCAAAAAAGTTATGGGTTGTGTCGTGGTGGGTGTGGTAGAACTGATTATATCATTTATAGGTTTCAAGAAACAATTCAATTACCTTTGAAAGGAAAGGTTCTGAAACTAGTTAACACCACCATGTGCTTTGTTGCAGCCAACAAACTTTCAGTAGACGCTATATTCGTGCTCACAAAGCAAGGTCACATGGCTTCTCTTCTATCCCGAAACCGCCCCAGCCCGCCTATATTCGCATTAACCGACGACGACAGCACGAGAATGGCTTTGAATCTTCAATGGGGAGTGTTTCCTCTGCGCATTGACCTCTCAGACGACATAGAAGCCAACATCTCAAGAGGAATCGAGCTAGTGAAGTCAAAGGGACTGGTCAAACAGGGGGACTTCGTGTTGATGGTCTCAGAGATCTCTCCAGCTCGAGCTGCATCAATGGCGTCCCAATCAATCCAGCTGAAATCCATCGTGTAGCACAAACAGAAAACCAAGGCAGGTCAGAGGTTTTATATACTGGAAACTGCTTTAGGATGCTTTGTAGTATGCTTTTTAGTGGTTTGTGATTGCACGTACCATATGTGGTGGCTACTCATTTATCTATCTCATTACATGTGAAACGACGTCGTTTTGTTACTTGTGGCCATGTCTGATAGGTTGCTTGCTGTAGTATAGCTAATATTTGGTGATTGAACAACTCTATTGAAATTATTGGCCATTGTTGATATGTTTGTAGAAAACGACAAAAGATGTGCCAAACTTTAGATCTGATTTTTAAAGTAATATTATTTATTTTATTATTAAATTTTAAGTTGTAGGAATTAGAAGTTGTA

mRNA sequence

TGCAGGTTTTCGTCAATGGCGGAGGTGAGTGCTATGATGATTCGTGCTTGTTGCAATTTCGTTGCTAAAACCTCTGTGTTAGATGTGAAACGATCGGATTCTCGTTTGTCGGTTATCGGTCGAACAGTTTGGTGCGATCGGGGTAGGATGAAGGGGAGCGTTGTAACTGGAGTTAAGGCGGCAATGCAGGTGGATTTGGAGGATGATGAGAGGTTGAAACGATTGGACGGTTTAGAGGCGACATTAGGGCTTGATGTTGTGGCGGAGAGGGAATTGAAGGAGAAAGGGTTTTTGGGGATGAGGAAGACGAAGTTTGTTTGTACGATTGGGCCTGCGTGTTGTGCGATTGAGGATTTGGAGAAGTTGGCTTTGGAAGGTATGAACATTGCGAGATTGAATATGTGTCACAACAAGAAGGAGTGGCACTATGACGTGATTAGGAAGATCAAGAAGTTGAATGAAAAAGGATTTTGTATTTCGGTGATGATTGATACTGAAGGAAGTCAGATTCATGTTGTTGATCATGGAGCTCCTTCCTCCGTCAAAGTAGAGGATGGTTCAAATTGGTTGTTTACTGCTGAGAAATTCGAGGGCTCTCGTCCATTTACTGTTCAAGCGAGCTATGAAGGCTTTTCTGAAGGTATCCAAGTGGGTGATTCAATTGTGATTGATGGGGGAATGGCATCCTTTGAAGTTATGGAAAAGATTGGGAATGATTTAAGCTGTAAGTGCACAGACCCTGGTTTGTTTCTTCCTCGAGCAAAATGCAGCTTTTGGAGGGATGGGAGGCTTGTTATGAGAAAACATGAGTCTCCCACGCTGTCAAGTAAGGATTGGTCCGACATTGAATTTGGAATCTCGGAAGGCGTCGACTTCATTGCCCTTTCCTTTGTGAACAGTGCAGATTCTGTCAAGCATCTAAAGAGCTACCTATCAGCAAAATCAGCAAAATCTGTGAGAGTATTGGCCAAGATTGAAAGCTTGGAGTCCCTTCAAAATCTAGAAGAAATCATAAAGGCTTCTGATGGAATCATGGTGGCTCGAGGGGATCTTGGGGTTGAAATTCCTCTTGAGCAGATACCAGCAGTCCAAGAAGAGATAACTAGTGTCTGTAGGGAGCTGAACAAACCAGTGATCGTAGCTTCTCAACTTCTAGAGTCGATGGTTGAATATCCTACTCCAACACGAGCTGAGGTTGCAGATGTTTCTGAAGTTGTCCGACAGTATGCCGATGCATTGATGCTGTCTGGAGAGTCTGCCATAGGAGCATATGGACAGAAAGCGCTTTCGGTTCTGCAGATAGCCAGCAGCCGAATGGAGTTATGGAGTCGTGAAGAAAATACGAAACATTTCCTTCCCCAACACCAACTTGGGGTGTCATTGCACGATCGCATTGCCGAAGAAATTTGCAATTCTGCAGCTGAATTGGCCAACAAACTTTCAGTAGACGCTATATTCGTGCTCACAAAGCAAGGTCACATGGCTTCTCTTCTATCCCGAAACCGCCCCAGCCCGCCTATATTCGCATTAACCGACGACGACAGCACGAGAATGGCTTTGAATCTTCAATGGGGAGTGTTTCCTCTGCGCATTGACCTCTCAGACGACATAGAAGCCAACATCTCAAGAGGAATCGAGCTAGTGAAGTCAAAGGGACTGGTCAAACAGGGGGACTTCGTGTTGATGGTCTCAGAGATCTCTCCAGCTCGAGCTGCATCAATGGCGTCCCAATCAATCCAGCTGAAATCCATCGTGTAGCACAAACAGAAAACCAAGGCAGTATGCTTTTTAGTGGTTTGTGATTGCACGTACCATATGTGGTGGCTACTCATTTATCTATCTCATTACATGTGAAACGACGTCGTTTTGTTACTTGTGGCCATGTCTGATAGGTTGCTTGCTGTAGTATAGCTAATATTTGGTGATTGAACAACTCTATTGAAATTATTGGCCATTGTTGATATGTTTGTAGAAAACGACAAAAGATGTGCCAAACTTTAGATCTGATTTTTAAAGTAATATTATTTATTTTATTATTAAATTTTAAGTTGTAGGAATTAGAAGTTGTA

Coding sequence (CDS)

ATGGCGGAGGTGAGTGCTATGATGATTCGTGCTTGTTGCAATTTCGTTGCTAAAACCTCTGTGTTAGATGTGAAACGATCGGATTCTCGTTTGTCGGTTATCGGTCGAACAGTTTGGTGCGATCGGGGTAGGATGAAGGGGAGCGTTGTAACTGGAGTTAAGGCGGCAATGCAGGTGGATTTGGAGGATGATGAGAGGTTGAAACGATTGGACGGTTTAGAGGCGACATTAGGGCTTGATGTTGTGGCGGAGAGGGAATTGAAGGAGAAAGGGTTTTTGGGGATGAGGAAGACGAAGTTTGTTTGTACGATTGGGCCTGCGTGTTGTGCGATTGAGGATTTGGAGAAGTTGGCTTTGGAAGGTATGAACATTGCGAGATTGAATATGTGTCACAACAAGAAGGAGTGGCACTATGACGTGATTAGGAAGATCAAGAAGTTGAATGAAAAAGGATTTTGTATTTCGGTGATGATTGATACTGAAGGAAGTCAGATTCATGTTGTTGATCATGGAGCTCCTTCCTCCGTCAAAGTAGAGGATGGTTCAAATTGGTTGTTTACTGCTGAGAAATTCGAGGGCTCTCGTCCATTTACTGTTCAAGCGAGCTATGAAGGCTTTTCTGAAGGTATCCAAGTGGGTGATTCAATTGTGATTGATGGGGGAATGGCATCCTTTGAAGTTATGGAAAAGATTGGGAATGATTTAAGCTGTAAGTGCACAGACCCTGGTTTGTTTCTTCCTCGAGCAAAATGCAGCTTTTGGAGGGATGGGAGGCTTGTTATGAGAAAACATGAGTCTCCCACGCTGTCAAGTAAGGATTGGTCCGACATTGAATTTGGAATCTCGGAAGGCGTCGACTTCATTGCCCTTTCCTTTGTGAACAGTGCAGATTCTGTCAAGCATCTAAAGAGCTACCTATCAGCAAAATCAGCAAAATCTGTGAGAGTATTGGCCAAGATTGAAAGCTTGGAGTCCCTTCAAAATCTAGAAGAAATCATAAAGGCTTCTGATGGAATCATGGTGGCTCGAGGGGATCTTGGGGTTGAAATTCCTCTTGAGCAGATACCAGCAGTCCAAGAAGAGATAACTAGTGTCTGTAGGGAGCTGAACAAACCAGTGATCGTAGCTTCTCAACTTCTAGAGTCGATGGTTGAATATCCTACTCCAACACGAGCTGAGGTTGCAGATGTTTCTGAAGTTGTCCGACAGTATGCCGATGCATTGATGCTGTCTGGAGAGTCTGCCATAGGAGCATATGGACAGAAAGCGCTTTCGGTTCTGCAGATAGCCAGCAGCCGAATGGAGTTATGGAGTCGTGAAGAAAATACGAAACATTTCCTTCCCCAACACCAACTTGGGGTGTCATTGCACGATCGCATTGCCGAAGAAATTTGCAATTCTGCAGCTGAATTGGCCAACAAACTTTCAGTAGACGCTATATTCGTGCTCACAAAGCAAGGTCACATGGCTTCTCTTCTATCCCGAAACCGCCCCAGCCCGCCTATATTCGCATTAACCGACGACGACAGCACGAGAATGGCTTTGAATCTTCAATGGGGAGTGTTTCCTCTGCGCATTGACCTCTCAGACGACATAGAAGCCAACATCTCAAGAGGAATCGAGCTAGTGAAGTCAAAGGGACTGGTCAAACAGGGGGACTTCGTGTTGATGGTCTCAGAGATCTCTCCAGCTCGAGCTGCATCAATGGCGTCCCAATCAATCCAGCTGAAATCCATCGTGTAG

Protein sequence

MAEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGSVVTGVKAAMQVDLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADSVKHLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV
Homology
[Close]
BLAST of Carg08103 vs. NCBI nr
Match: XP_022942487.1 (plastidial pyruvate kinase 1, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1040.0 bits (2688), Expect = 3.0e-300
Identity = 573/580 (98.79%), Postives = 574/580 (98.97%), Query Frame = 0 

Query: 1   MAEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGSVVTGVKAAMQVD 60
           M EVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKG VVTGVKAAMQVD
Sbjct: 1   MTEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGRVVTGVKAAMQVD 60

Query: 61  LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALE 120
           LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALE
Sbjct: 61  LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED 180
           GMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAP SVKVED
Sbjct: 121 GMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPFSVKVED 180

Query: 181 GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT 240
           GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT
Sbjct: 181 GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT 240

Query: 241 DPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSAXXXX 300
           DPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSAXXXX
Sbjct: 241 DPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSAXXXX 300

Query: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARGDLGVEIPLEQIPAVQE 360
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARGDLGVEIPLEQIPAVQE
Sbjct: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARGDLGVEIPLEQIPAVQE 360

Query: 361 EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYG 420
           EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALML GESAIG+YG
Sbjct: 361 EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLCGESAIGSYG 420

Query: 421 QKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI 480
           QKALSVLQIASSRMELWS EENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI
Sbjct: 421 QKALSVLQIASSRMELWSGEENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI 480

Query: 481 FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGI 540
           FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGI
Sbjct: 481 FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGI 540

Query: 541 ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 581
           ELVKSKGLVKQGDFVLMVSEIS ARAASMASQSIQLKSIV
Sbjct: 541 ELVKSKGLVKQGDFVLMVSEISAARAASMASQSIQLKSIV 580

[Close]
BLAST of Carg08103 vs. NCBI nr
Match: XP_022975726.1 (plastidial pyruvate kinase 1, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1020.8 bits (2638), Expect = 1.9e-294
Identity = 564/580 (97.24%), Postives = 568/580 (97.93%), Query Frame = 0 

Query: 1   MAEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGSVVTGVKAAMQVD 60
           MAEVSAMMIRA CNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKG VVTGVKA MQVD
Sbjct: 1   MAEVSAMMIRARCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGRVVTGVKATMQVD 60

Query: 61  LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALE 120
           LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCA EDLEKLALE
Sbjct: 61  LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCATEDLEKLALE 120

Query: 121 GMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED 180
           GMNIARLNMCHN KEWHY VIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED
Sbjct: 121 GMNIARLNMCHNTKEWHYGVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED 180

Query: 181 GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT 240
           GSNWLFTAEKFEGSRPFTVQASY GFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT
Sbjct: 181 GSNWLFTAEKFEGSRPFTVQASYGGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT 240

Query: 241 DPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSAXXXX 300
           DPGLFLPRAKCSFWRDGRLV+RKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSAXXXX
Sbjct: 241 DPGLFLPRAKCSFWRDGRLVVRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSAXXXX 300

Query: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARGDLGVEIPLEQIPAVQE 360
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX  ASDGIMVARGDLGVEIPLEQIPAVQE
Sbjct: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXASDGIMVARGDLGVEIPLEQIPAVQE 360

Query: 361 EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYG 420
           EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSE VRQYADALML GESAIG+YG
Sbjct: 361 EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGSYG 420

Query: 421 QKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI 480
           QKALSVLQIASSRMELWSREEN KHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI
Sbjct: 421 QKALSVLQIASSRMELWSREENRKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI 480

Query: 481 FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGI 540
           FVLTKQGHMASLLSRNRPSPPIFALT++DSTRMALNLQWGVFPLRIDLSDDIEANISRGI
Sbjct: 481 FVLTKQGHMASLLSRNRPSPPIFALTNEDSTRMALNLQWGVFPLRIDLSDDIEANISRGI 540

Query: 541 ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 581
           ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV
Sbjct: 541 ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 580

[Close]
BLAST of Carg08103 vs. NCBI nr
Match: XP_023515955.1 (plastidial pyruvate kinase 1, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1018.1 bits (2631), Expect = 1.2e-293
Identity = 562/580 (96.90%), Postives = 567/580 (97.76%), Query Frame = 0 

Query: 1   MAEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGSVVTGVKAAMQVD 60
           MAEVSAMMIRA CNFVA   VLDVKRSDSRLSVIGRTVWCDRGRMKG VVTGVKAAMQ+D
Sbjct: 1   MAEVSAMMIRARCNFVAINPVLDVKRSDSRLSVIGRTVWCDRGRMKGRVVTGVKAAMQMD 60

Query: 61  LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALE 120
           LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAI+DLEKLALE
Sbjct: 61  LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIDDLEKLALE 120

Query: 121 GMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED 180
           GMNIARLNMCHN KEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED
Sbjct: 121 GMNIARLNMCHNTKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED 180

Query: 181 GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT 240
           GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT
Sbjct: 181 GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT 240

Query: 241 DPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSAXXXX 300
           DPGLFLPRAKCSFWR+GRLV+RKHESPTLSSKDWSDIEFGISEGVDFIALSFV   XXXX
Sbjct: 241 DPGLFLPRAKCSFWRNGRLVVRKHESPTLSSKDWSDIEFGISEGVDFIALSFVXXXXXXX 300

Query: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARGDLGVEIPLEQIPAVQE 360
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARGDLGVEIPLEQIPAVQE
Sbjct: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARGDLGVEIPLEQIPAVQE 360

Query: 361 EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYG 420
           EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSE VRQYADALML GESAIG+YG
Sbjct: 361 EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGSYG 420

Query: 421 QKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI 480
           QKALSVLQIASSRMELWSREEN KHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI
Sbjct: 421 QKALSVLQIASSRMELWSREENRKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI 480

Query: 481 FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGI 540
           FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRM LNLQWGVFPLRIDLSDDIEANISRGI
Sbjct: 481 FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMGLNLQWGVFPLRIDLSDDIEANISRGI 540

Query: 541 ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 581
           ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV
Sbjct: 541 ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 580

[Close]
BLAST of Carg08103 vs. NCBI nr
Match: XP_022942488.1 (plastidial pyruvate kinase 1, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 955.7 bits (2469), Expect = 7.3e-275
Identity = 530/536 (98.88%), Postives = 531/536 (99.07%), Query Frame = 0 

Query: 45  MKGSVVTGVKAAMQVDLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTI 104
           MKG VVTGVKAAMQVDLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTI
Sbjct: 1   MKGRVVTGVKAAMQVDLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTI 60

Query: 105 GPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQ 164
           GPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQ
Sbjct: 61  GPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQ 120

Query: 165 IHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMAS 224
           IHVVDHGAP SVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMAS
Sbjct: 121 IHVVDHGAPFSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMAS 180

Query: 225 FEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEG 284
           FEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEG
Sbjct: 181 FEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEG 240

Query: 285 VDFIALSFVNSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARG 344
           VDFIALSFVNSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARG
Sbjct: 241 VDFIALSFVNSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARG 300

Query: 345 DLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQY 404
           DLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQY
Sbjct: 301 DLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQY 360

Query: 405 ADALMLSGESAIGAYGQKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEI 464
           ADALML GESAIG+YGQKALSVLQIASSRMELWS EENTKHFLPQHQLGVSLHDRIAEEI
Sbjct: 361 ADALMLCGESAIGSYGQKALSVLQIASSRMELWSGEENTKHFLPQHQLGVSLHDRIAEEI 420

Query: 465 CNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPL 524
           CNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPL
Sbjct: 421 CNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPL 480

Query: 525 RIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 581
           RIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEIS ARAASMASQSIQLKSIV
Sbjct: 481 RIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISAARAASMASQSIQLKSIV 536

[Close]
BLAST of Carg08103 vs. NCBI nr
Match: XP_023515961.1 (plastidial pyruvate kinase 1, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 943.7 bits (2438), Expect = 2.9e-271
Identity = 522/536 (97.39%), Postives = 527/536 (98.32%), Query Frame = 0 

Query: 45  MKGSVVTGVKAAMQVDLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTI 104
           MKG VVTGVKAAMQ+DLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTI
Sbjct: 1   MKGRVVTGVKAAMQMDLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTI 60

Query: 105 GPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQ 164
           GPACCAI+DLEKLALEGMNIARLNMCHN KEWHYDVIRKIKKLNEKGFCISVMIDTEGSQ
Sbjct: 61  GPACCAIDDLEKLALEGMNIARLNMCHNTKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQ 120

Query: 165 IHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMAS 224
           IHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMAS
Sbjct: 121 IHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMAS 180

Query: 225 FEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEG 284
           FEVMEKIGNDLSCKCTDPGLFLPRAKCSFWR+GRLV+RKHESPTLSSKDWSDIEFGISEG
Sbjct: 181 FEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRNGRLVVRKHESPTLSSKDWSDIEFGISEG 240

Query: 285 VDFIALSFVNSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARG 344
           VDFIALSFV   XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARG
Sbjct: 241 VDFIALSFVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARG 300

Query: 345 DLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQY 404
           DLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSE VRQY
Sbjct: 301 DLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEAVRQY 360

Query: 405 ADALMLSGESAIGAYGQKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEI 464
           ADALML GESAIG+YGQKALSVLQIASSRMELWSREEN KHFLPQHQLGVSLHDRIAEEI
Sbjct: 361 ADALMLCGESAIGSYGQKALSVLQIASSRMELWSREENRKHFLPQHQLGVSLHDRIAEEI 420

Query: 465 CNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPL 524
           CNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRM LNLQWGVFPL
Sbjct: 421 CNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMGLNLQWGVFPL 480

Query: 525 RIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 581
           RIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV
Sbjct: 481 RIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 536

[Close]
BLAST of Carg08103 vs. TAIR10
Match: AT3G22960.1 (Pyruvate kinase family protein)

HSP 1 Score: 543.9 bits (1400), Expect = 1.2e-154
Identity = 273/487 (56.06%), Postives = 340/487 (69.82%), Query Frame = 0 

Query: 79  LDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKKEWHY 138
           +D V E ELKE GF   R+TK +CTIGPA C  E LE LA+ GMN+ARLNMCH  ++WH 
Sbjct: 100 VDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHR 159

Query: 139 DVIRKIKKLN-EKGFCISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRP- 198
            VIR +++LN EKGF +++M+DTEGS+IH+ D G  +S K EDG  W FT   F+ SRP 
Sbjct: 160 GVIRSVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPE 219

Query: 199 FTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRD 258
            T+  SY+GF+E ++VGD +++DGGM  FEV+EKIG D+ C CTDPGL LPRA  +FWRD
Sbjct: 220 RTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRD 279

Query: 259 GRLVMRKHES-PTLSSKDWSDIEFGISEGVDFIALSFVNSA-XXXXXXXXXXXXXXXXXX 318
           G LV  ++   PT+SSKDW DI+FGI+EGVDFIA+SFV SA                   
Sbjct: 280 GSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEI 339

Query: 319 XXXXXXXXXXXXXXXXXXIKASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVI 378
                             I ASDG MVARGDLG +IPLEQ+PA Q+ I  VCR LNKPVI
Sbjct: 340 GVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVI 399

Query: 379 VASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSRM 438
           VASQLLESM+EYPTPTRAEVADVSE VRQ +DALMLSGESA+G +  KAL+VL+  S R+
Sbjct: 400 VASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRI 459

Query: 439 ELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLLS 498
           E W REE     +P   +G S  D+I+EEICNSAA++AN L VDA+FV T  GHMASL+S
Sbjct: 460 ERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVS 519

Query: 499 RNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDF 558
           R RP  PIFA T   S R  LNLQWG+ P R+  SDD+E+N+++   L+KS+G++K GD 
Sbjct: 520 RCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDL 579

Query: 559 VLMVSEI 562
           V+ VS++
Sbjct: 580 VIAVSDM 586

[Close]
BLAST of Carg08103 vs. TAIR10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1)

HSP 1 Score: 277.3 bits (708), Expect = 2.1e-74
Identity = 170/486 (34.98%), Postives = 261/486 (53.70%), Query Frame = 0 

Query: 96  RKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLN--EKGFC 155
           RKTK VCT+GP+    E + KLA  GMN+AR+NM H     H  VI  +K+ N   K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 156 ISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVG 215
           I++M+DT+G ++   D   P  + ++ G  + FT E+   S P  V  +Y+ F   ++ G
Sbjct: 170 IAIMLDTKGPEVRSGD--LPQPIMLDPGQEFTFTIER-GVSTPSCVSVNYDDFVNDVEAG 229

Query: 216 DSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKD 275
           D +++DGGM SF V  K  + + C+  D G    R      R   +  +    P+++ KD
Sbjct: 230 DMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSR------RHLNVRGKSATLPSITEKD 289

Query: 276 WSDIEFGISEGVDFIALSFVNSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI 335
           W DI+FG+   VDF A+SFV  A                                    I
Sbjct: 290 WEDIKFGVENKVDFYAVSFVKDA-QVVHELKKYLQNSGADIHVIVKIESADSIPNLHSII 349

Query: 336 KASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAE 395
            ASDG MVARGDLG E+P+E++P +QEEI ++CR + K VIVA+ +LESM+ +PTPTRAE
Sbjct: 350 TASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAE 409

Query: 396 VADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSRMELWSREENTKHFLPQHQLG 455
           V+D++  VR+ ADA+MLSGE+A G +  KA  V+   + R E       T   +P + LG
Sbjct: 410 VSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTE----ATITSGEMPPN-LG 469

Query: 456 VSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRM 515
            +  + ++E     A  ++N L    + V T+ G MA LLS  RPS  I+A T++   + 
Sbjct: 470 QAFKNHMSEMFAYHATMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQ 529

Query: 516 ALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMASQS 575
            L L  GV P+ ++ +DD E   +  +  +  +G+VK+G+ + +V   +     S ++ +
Sbjct: 530 RLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPIWRSQSTHN 579

Query: 576 IQLKSI 580
           IQ++ +
Sbjct: 590 IQVRKV 579

[Close]
BLAST of Carg08103 vs. TAIR10
Match: AT1G32440.1 (plastidial pyruvate kinase 3)

HSP 1 Score: 268.5 bits (685), Expect = 9.8e-72
Identity = 173/488 (35.45%), Postives = 258/488 (52.87%), Query Frame = 0 

Query: 96  RKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNE--KGFC 155
           RKTK VCTIGP+  + E + KLA  GMN+ARLNM H     H   I  +K+ N       
Sbjct: 99  RKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLVKEYNSLFVDKA 158

Query: 156 ISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVG 215
           I++M+DT+G ++   D   P  + +E+G  + FT ++    +  TV  +Y+ F   ++VG
Sbjct: 159 IAIMLDTKGPEVRSGD--VPQPIFLEEGQEFNFTIKRGVSLKD-TVSVNYDDFVNDVEVG 218

Query: 216 DSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKD 275
           D +++DGGM S  V  K  + + C   D G    R      R   +  +    P+++ KD
Sbjct: 219 DILLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSR------RHLNVRGKSATLPSITDKD 278

Query: 276 WSDIEFGISEGVDFIALSFVNSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI 335
           W DI+FG+   VDF A+SFV  A                                    I
Sbjct: 279 WEDIKFGVDNQVDFYAVSFVKDA-KVVHELKNYLKTCSADISVIVKIESADSIKNLPSII 338

Query: 336 KASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAE 395
            A DG MVARGDLG E+P+E++P +QEEI   CR ++KPVIVA+ +LESM+ +PTPTRAE
Sbjct: 339 SACDGAMVARGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNMLESMINHPTPTRAE 398

Query: 396 VADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSRME--LWSREENTKHFLPQHQ 455
           V+D++  VR+ ADA+MLSGE+A G +  KA++V+   + R E  L  R   ++    +  
Sbjct: 399 VSDIAIAVREGADAIMLSGETAHGKFPLKAVNVMHTVALRTEASLPVRTSASRTTAYKGH 458

Query: 456 LGVSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDST 515
           +G        +     A+ +AN LS   + V T+ G MA LLS  RPS  IFA T+    
Sbjct: 459 MG--------QMFAFHASIMANTLS-SPLIVFTRTGSMAVLLSHYRPSATIFAFTNQRRI 518

Query: 516 RMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMAS 575
              L L  GV P+ ++ SDD E   +R ++L++ + ++K+G  V +V   S       ++
Sbjct: 519 MQRLALYQGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPIWREEST 567

Query: 576 QSIQLKSI 580
             IQ++ I
Sbjct: 579 HLIQVRKI 567

[Close]
BLAST of Carg08103 vs. TAIR10
Match: AT3G04050.1 (Pyruvate kinase family protein)

HSP 1 Score: 193.7 bits (491), Expect = 3.1e-49
Identity = 157/502 (31.27%), Postives = 231/502 (46.02%), Query Frame = 0 

Query: 97  KTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCI-- 156
           KTK VCT+GPA  ++E +EKL   GMN+AR N  H    +H + +  ++   E   CI  
Sbjct: 17  KTKIVCTLGPASRSVEMVEKLLKAGMNVARFNFSHGSHSYHQETLDNLRTAME-NTCIPC 76

Query: 157 SVMIDTEGSQIHV--VDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQV 216
           +VM+DT+G +I    +  G P  V++  G     + +        T+  SY+  +E ++ 
Sbjct: 77  AVMLDTKGPEIRTGFLKEGKP--VELIQGQEITISTDYTMEGDSNTISMSYKKLAEDLKS 136

Query: 217 GDSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKH-------- 276
           GD I+   G  S  V       LSC   D  L L RA+C       L  RK+        
Sbjct: 137 GDVILCSDGTISLTV-------LSC---DKNLGLVRARCE--NSAVLGERKNVNLPGIVV 196

Query: 277 ESPTLSSKDWSDI-EFGISEGVDFIALSFVNSAXXXXXXXXXXXXXXXXXXXXXXXXXXX 336
           + PTL+ KD  DI ++G+   +D IALSFV                              
Sbjct: 197 DLPTLTEKDQEDILQWGVPNKIDIIALSFVRKG-SDLVEVRKLLGENAKSIMLMSKVENQ 256

Query: 337 XXXXXXXXXIKASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESM 396
                    ++ SD  MVARGDLG+EIP+E++   Q+ +      L KP++ A+Q+LESM
Sbjct: 257 EGVMNFDKILEYSDAFMVARGDLGMEIPIEKMFLAQKMMIQKANALGKPIVTATQMLESM 316

Query: 397 VEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSRMELWSREENT 456
            + P PTRAE  DV+  V    D +MLSGE+A GA+ + A+  +       E +  + +T
Sbjct: 317 TKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAHPETAVLTMSRICKEAEDFI-DYDT 376

Query: 457 KHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIF 516
            H   + Q  VSL     E +  SA   A  L   AI VLTK G+   L+++ RPS PI 
Sbjct: 377 MH--KKIQDIVSLPLSPIESLAASAVSTARSLCAAAIVVLTKGGYTVELVAKYRPSVPIL 436

Query: 517 ALTDDDSTRMALNLQW-----------------GVFPL-------RIDLSDDIEANISRG 562
           ++   + TR   + +W                 G+ P+       R    D  E  I   
Sbjct: 437 SVIVPEITRTD-DFEWSCSESAAHVARRGLIYRGIVPVMATGASARSSNKDSTEETIQFA 496

[Close]
BLAST of Carg08103 vs. TAIR10
Match: AT5G63680.1 (Pyruvate kinase family protein)

HSP 1 Score: 189.5 bits (480), Expect = 5.8e-48
Identity = 144/494 (29.15%), Postives = 229/494 (46.36%), Query Frame = 0 

Query: 97  KTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIK-KLNEKGFCIS 156
           KTK VCT+GPA  ++  +EKL   GMN+AR N  H   E+H + +  ++  +   G   +
Sbjct: 21  KTKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSHEYHQETLDNLRTAMQNTGILAA 80

Query: 157 VMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDS 216
           VM+DT+G +I        + +++++G     T +        T+  SY+     ++ G++
Sbjct: 81  VMLDTKGPEIRTGFLKDGNPIQLKEGQEITITTDYDIKGDEKTISMSYKKLPVDVKPGNT 140

Query: 217 IVIDGGMASFEVM--EKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKD 276
           I+   G  S  V+  +     + C+C +  +   R   +    G +V    + PTL+ KD
Sbjct: 141 ILCADGSISLAVVSCDPNAGTVICRCENTAMLGERKNVNL--PGVVV----DLPTLTDKD 200

Query: 277 WSDI-EFGISEGVDFIALSFVNSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 336
             DI ++G+   +D IALSFV                                       
Sbjct: 201 VEDILKWGVPNNIDMIALSFVRKG-SDLVNVRKVLGSHSKSIMLMSKVENQEGVLNFDEI 260

Query: 337 IKASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRA 396
           ++ +D  MVARGDLG+EIP+E+I   Q+ +   C    KPV+ A+Q+LESM++ P PTRA
Sbjct: 261 LRETDAFMVARGDLGMEIPIEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRA 320

Query: 397 EVADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSRMELWSREENT--KHFLPQH 456
           E  DV+  V    D +MLSGESA GAY + A+  +       E  S + NT  K  +   
Sbjct: 321 EATDVANAVLDGTDCVMLSGESAAGAYPEIAVKTMAKICIEAES-SLDYNTIFKEMIRAT 380

Query: 457 QLGVSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFAL----- 516
            L +S      E + +SA   ANK     I VLT+ G  A L+++ RP+ PI ++     
Sbjct: 381 PLPMS----TLESLASSAVRTANKAKAKLIIVLTRGGTTAKLVAKYRPAVPILSVVVPVF 440

Query: 517 ---------TDDDSTRMALNLQWGVFPL------RIDLSDDIEANISRGIELVKSKGLVK 565
                    +D+   R +L +  G+ P+      +   S+  E  I   ++    KGL  
Sbjct: 441 TSDTFNWSCSDESPARHSL-IYRGLIPVLGEGSAKATDSESTEEIIESALKSATEKGLCN 500

[Close]
BLAST of Carg08103 vs. Swiss-Prot
Match: sp|Q9LIK0|PKP1_ARATH (Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP1 PE=1 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 2.2e-153
Identity = 273/487 (56.06%), Postives = 340/487 (69.82%), Query Frame = 0 

Query: 79  LDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKKEWHY 138
           +D V E ELKE GF   R+TK +CTIGPA C  E LE LA+ GMN+ARLNMCH  ++WH 
Sbjct: 100 VDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHR 159

Query: 139 DVIRKIKKLN-EKGFCISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRP- 198
            VIR +++LN EKGF +++M+DTEGS+IH+ D G  +S K EDG  W FT   F+ SRP 
Sbjct: 160 GVIRSVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPE 219

Query: 199 FTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRD 258
            T+  SY+GF+E ++VGD +++DGGM  FEV+EKIG D+ C CTDPGL LPRA  +FWRD
Sbjct: 220 RTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRD 279

Query: 259 GRLVMRKHES-PTLSSKDWSDIEFGISEGVDFIALSFVNSA-XXXXXXXXXXXXXXXXXX 318
           G LV  ++   PT+SSKDW DI+FGI+EGVDFIA+SFV SA                   
Sbjct: 280 GSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEI 339

Query: 319 XXXXXXXXXXXXXXXXXXIKASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVI 378
                             I ASDG MVARGDLG +IPLEQ+PA Q+ I  VCR LNKPVI
Sbjct: 340 GVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVI 399

Query: 379 VASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSRM 438
           VASQLLESM+EYPTPTRAEVADVSE VRQ +DALMLSGESA+G +  KAL+VL+  S R+
Sbjct: 400 VASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRI 459

Query: 439 ELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLLS 498
           E W REE     +P   +G S  D+I+EEICNSAA++AN L VDA+FV T  GHMASL+S
Sbjct: 460 ERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVS 519

Query: 499 RNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDF 558
           R RP  PIFA T   S R  LNLQWG+ P R+  SDD+E+N+++   L+KS+G++K GD 
Sbjct: 520 RCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDL 579

Query: 559 VLMVSEI 562
           V+ VS++
Sbjct: 580 VIAVSDM 586

[Close]
BLAST of Carg08103 vs. Swiss-Prot
Match: sp|Q43117|KPYA_RICCO (Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 8.3e-153
Identity = 269/491 (54.79%), Postives = 346/491 (70.47%), Query Frame = 0 

Query: 75  ATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKK 134
           +++ +D V E ELKE GF   R+TK VCTIGPA C  E+LE LA+ GMN+AR+NMCH  +
Sbjct: 83  SSIEVDAVTETELKENGFRSTRRTKLVCTIGPATCGFEELEALAVGGMNVARINMCHGTR 142

Query: 135 EWHYDVIRKIKKLN-EKGFCISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEG 194
           EWH  VI ++++LN EKGF +++M+DTEGS+IH+ D G  SS K EDG  W F+   ++ 
Sbjct: 143 EWHKSVIERVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAYDS 202

Query: 195 SRP-FTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCS 254
            RP  T+  +Y+GF+E ++VGD +++DGGM  FEV+EKIG D+ C+CTDPGL LPRA  +
Sbjct: 203 PRPERTINVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCRCTDPGLLLPRANLT 262

Query: 255 FWRDGRLVMRKHES-PTLSSKDWSDIEFGISEGVDFIALSFVNSA-XXXXXXXXXXXXXX 314
           FWRDG LV  ++   PT+SSKDW DI+FGI+EGVDFIA+SFV SA               
Sbjct: 263 FWRDGSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAISFVKSAEVINHLKSYIAARSR 322

Query: 315 XXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELN 374
                                 I+ASDG MVARGDLG +IPLEQ+P+ Q+ I  VCR+LN
Sbjct: 323 DSDIAVIAKIESIDSLKNLEEIIRASDGAMVARGDLGAQIPLEQVPSAQQNIVQVCRQLN 382

Query: 375 KPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIA 434
           KPVIVASQLLESM+EYPTPTRAEVADVSE VRQ ADALMLSGESA+G Y +KAL+VL+  
Sbjct: 383 KPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRADALMLSGESAMGQYPEKALAVLRSV 442

Query: 435 SSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMA 494
           S R+E W REE     +    +G +  D I+EEICNSAA++AN L VDA+FV TK GHMA
Sbjct: 443 SVRIEKWWREEKHHEAMELPAIGSTYSDSISEEICNSAAKMANNLGVDALFVYTKDGHMA 502

Query: 495 SLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVK 554
           SLLSR RP  PIFA T   S R  LNLQWG+ P R+  +DD+E+N+++   L+K++G++K
Sbjct: 503 SLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFADDMESNLNKTFSLLKARGMIK 562

Query: 555 QGDFVLMVSEI 562
            GD V+ VS++
Sbjct: 563 SGDLVIAVSDM 573

[Close]
BLAST of Carg08103 vs. Swiss-Prot
Match: sp|Q40545|KPYA_TOBAC (Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 1.4e-147
Identity = 263/491 (53.56%), Postives = 341/491 (69.45%), Query Frame = 0 

Query: 75  ATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKK 134
           +++ +D V E ELKE GF   R+TK +CTIGPA C  E LE+LA  GMN+AR+NMCH  +
Sbjct: 93  SSIEVDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLERLAEGGMNVARINMCHGTR 152

Query: 135 EWHYDVIRKIKKLN-EKGFCISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEG 194
           EWH  VI ++++LN EKGF +++M+DTEGS+IH+ D G  SS K EDG  W FT   F+ 
Sbjct: 153 EWHRMVIERLRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWNFTVRSFDP 212

Query: 195 SRP-FTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCS 254
             P  TV  +Y+GF+E ++VGD +++DGGM  FEV+EKIG D+ C CTDPGL LPRA  +
Sbjct: 213 PLPERTVTVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLT 272

Query: 255 FWRDGRLVMRKHES-PTLSSKDWSDIEFGISEGVDFIALSFVNSA-XXXXXXXXXXXXXX 314
           FWRDG+LV  ++   PT+SSKDW DI+FGI+EGVDFIA+SFV SA               
Sbjct: 273 FWRDGKLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVIKHLKSYIQARAR 332

Query: 315 XXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELN 374
                                 I+ASDG MVARGDLG +IPLEQ+P+ Q++I  +CR+LN
Sbjct: 333 DSDISVIAKIESIDSLKNLEEIIQASDGAMVARGDLGAQIPLEQVPSEQQKIVQICRQLN 392

Query: 375 KPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIA 434
           +PVIVASQLLESM+EYP PTRAEVADVSE VRQ  DALMLSGESA+G + +KAL+VL+  
Sbjct: 393 RPVIVASQLLESMIEYPIPTRAEVADVSEAVRQRGDALMLSGESAMGQFPEKALTVLRSV 452

Query: 435 SSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMA 494
           S R+E   RE+     +    +  S  D I+EEICNSAA++AN L VDA+FV TK GHMA
Sbjct: 453 SLRIERMWREQKRHEVIELPSIASSFSDSISEEICNSAAKMANNLEVDALFVYTKNGHMA 512

Query: 495 SLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVK 554
           SLLSR RP  PIFA T   S R  LNLQWG+ P R+  SDD+E+N+++   L+K++G++K
Sbjct: 513 SLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLMPFRLSFSDDMESNLNKTFSLLKARGMIK 572

Query: 555 QGDFVLMVSEI 562
            GD ++ VS++
Sbjct: 573 SGDLIIAVSDM 583

[Close]
BLAST of Carg08103 vs. Swiss-Prot
Match: sp|Q40546|KPYG_TOBAC (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.4e-75
Identity = 176/486 (36.21%), Postives = 265/486 (54.53%), Query Frame = 0 

Query: 96  RKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNE--KGFC 155
           RKTK VCTIGP+  + E + KLA  GMN+ARLNM H     H   I  +K+ N   +   
Sbjct: 91  RKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFEDKV 150

Query: 156 ISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVG 215
           I++M+DT+G +  V+    P  + +++G  + F+ ++   +   TV  +Y+ F   ++ G
Sbjct: 151 IAIMLDTKGPE--VISGDVPKPILLKEGQEFNFSIKRGVSTED-TVSVNYDDFINDVEAG 210

Query: 216 DSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKD 275
           D +++DGGM S  V  K  + + C+  D G    R      R   +  +    P+++ KD
Sbjct: 211 DILLVDGGMMSLAVKSKTSDIVKCEVIDGGELKSR------RHLNVRGKSATLPSITEKD 270

Query: 276 WSDIEFGISEGVDFIALSFVNSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI 335
           W DI+FG++  VDF A+SFV  A                                    I
Sbjct: 271 WDDIKFGVNNQVDFYAVSFVKDA-KVVHELKDYLKSCNADIHVIVKIESADSIPNLHSII 330

Query: 336 KASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAE 395
            ASDG MVARGDLG E+P+E++P +QE+I   C+ + KPVIVA+ +LESM+++PTPTRAE
Sbjct: 331 SASDGAMVARGDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMIDHPTPTRAE 390

Query: 396 VADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSRMELWSREENTKHFLPQHQLG 455
           V+D+S  VR+ ADA+MLSGE+A G Y  KA+ V+ I + R E  S +++T    P     
Sbjct: 391 VSDISIAVREGADAVMLSGETAHGKYPLKAVKVMHIVALRTES-SLQKSTSS--PSQSAA 450

Query: 456 VSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRM 515
              H  + E     ++ +AN LS   I V T+ G MA +LS NRPS  +FA T+++  + 
Sbjct: 451 YKSH--MGEMFAFHSSSMANTLST-PIIVFTRTGSMAIILSHNRPSSTVFAFTNNERVKQ 510

Query: 516 ALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMASQS 575
            L L  GV P+ ++ S D E   SR I+L+ SK LVK G +V +V   +       ++  
Sbjct: 511 RLALYHGVVPIYMEFSSDAEETFSRAIKLLLSKSLVKDGQYVTLVQSGAQPIWRRHSTHH 560

Query: 576 IQLKSI 580
           IQ++ +
Sbjct: 571 IQVRKV 560

[Close]
BLAST of Carg08103 vs. Swiss-Prot
Match: sp|Q9FLW9|PKP2_ARATH (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 3.8e-73
Identity = 170/486 (34.98%), Postives = 261/486 (53.70%), Query Frame = 0 

Query: 96  RKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLN--EKGFC 155
           RKTK VCT+GP+    E + KLA  GMN+AR+NM H     H  VI  +K+ N   K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 156 ISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVG 215
           I++M+DT+G ++   D   P  + ++ G  + FT E+   S P  V  +Y+ F   ++ G
Sbjct: 170 IAIMLDTKGPEVRSGD--LPQPIMLDPGQEFTFTIER-GVSTPSCVSVNYDDFVNDVEAG 229

Query: 216 DSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKD 275
           D +++DGGM SF V  K  + + C+  D G    R      R   +  +    P+++ KD
Sbjct: 230 DMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSR------RHLNVRGKSATLPSITEKD 289

Query: 276 WSDIEFGISEGVDFIALSFVNSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI 335
           W DI+FG+   VDF A+SFV  A                                    I
Sbjct: 290 WEDIKFGVENKVDFYAVSFVKDA-QVVHELKKYLQNSGADIHVIVKIESADSIPNLHSII 349

Query: 336 KASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAE 395
            ASDG MVARGDLG E+P+E++P +QEEI ++CR + K VIVA+ +LESM+ +PTPTRAE
Sbjct: 350 TASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAE 409

Query: 396 VADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSRMELWSREENTKHFLPQHQLG 455
           V+D++  VR+ ADA+MLSGE+A G +  KA  V+   + R E       T   +P + LG
Sbjct: 410 VSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTE----ATITSGEMPPN-LG 469

Query: 456 VSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRM 515
            +  + ++E     A  ++N L    + V T+ G MA LLS  RPS  I+A T++   + 
Sbjct: 470 QAFKNHMSEMFAYHATMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQ 529

Query: 516 ALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMASQS 575
            L L  GV P+ ++ +DD E   +  +  +  +G+VK+G+ + +V   +     S ++ +
Sbjct: 530 RLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPIWRSQSTHN 579

Query: 576 IQLKSI 580
           IQ++ +
Sbjct: 590 IQVRKV 579

[Close]
BLAST of Carg08103 vs. TrEMBL
Match: tr|A0A1S3BYK3|A0A1S3BYK3_CUCME (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103494833 PE=3 SV=1)

HSP 1 Score: 943.3 bits (2437), Expect = 2.5e-271
Identity = 487/582 (83.68%), Postives = 516/582 (88.66%), Query Frame = 0 

Query: 1   MAEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIG-RTVWCDRGRMKGSVVTGVKAAMQV 60
           MAEV+AMMIRA CNF+ +  VLDVKR   R+ V+G RTVWC+R RMKG V  GVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLAL 120
           DL+D+ERL+RL+GLEATLGLD+VAERELKEKGFLGMRKTK VCTIGPAC  IEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNKKEWHYDVIRKIKKLN-EKGFCISVMIDTEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHN +EWH D+I+KIKKLN EKGFCISVMID EGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCK 240
           EDGS WLFTAEKFEGSRPFTVQASYEGFSEGI+VGDSIVIDGGMA+FEV EKIGNDLSC+
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSAXX 300
           CTDPGLFLPRAKCSFWRDGRLV+R HESPTLSSKDWSDIEFGISEGVDFIALSFVNSA  
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARGDLGVEIPLEQIPAV 360
                                             I+ASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGA 420
           QEEIT VCRELNKPVI+ASQLLESMVEYPTPTRAEVADVSE VRQYADALMLSGESAIG+
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVD 480
           YGQKALSVLQ+ASSRMELWSREENTK+FLPQHQLGVSLHDRIAEEICNSAAELAN+LSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISR 540
           AIFVLT QGHMASLLSRNRP PPIFALTDDDSTRMALNLQWGVFPLRIDLS+DIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 581
           GIE+VKSKGLVKQGD VL+VSEISPARA SMASQSIQLK+IV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

[Close]
BLAST of Carg08103 vs. TrEMBL
Match: tr|E5GBH7|E5GBH7_CUCME (Pyruvate kinase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 943.3 bits (2437), Expect = 2.5e-271
Identity = 487/582 (83.68%), Postives = 516/582 (88.66%), Query Frame = 0 

Query: 1   MAEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIG-RTVWCDRGRMKGSVVTGVKAAMQV 60
           MAEV+AMMIRA CNF+ +  VLDVKR   R+ V+G RTVWC+R RMKG V  GVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLAL 120
           DL+D+ERL+RL+GLEATLGLD+VAERELKEKGFLGMRKTK VCTIGPAC  IEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNKKEWHYDVIRKIKKLN-EKGFCISVMIDTEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHN +EWH D+I+KIKKLN EKGFCISVMID EGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCK 240
           EDGS WLFTAEKFEGSRPFTVQASYEGFSEGI+VGDSIVIDGGMA+FEV EKIGNDLSC+
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSAXX 300
           CTDPGLFLPRAKCSFWRDGRLV+R HESPTLSSKDWSDIEFGISEGVDFIALSFVNSA  
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARGDLGVEIPLEQIPAV 360
                                             I+ASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGA 420
           QEEIT VCRELNKPVI+ASQLLESMVEYPTPTRAEVADVSE VRQYADALMLSGESAIG+
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVD 480
           YGQKALSVLQ+ASSRMELWSREENTK+FLPQHQLGVSLHDRIAEEICNSAAELAN+LSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISR 540
           AIFVLT QGHMASLLSRNRP PPIFALTDDDSTRMALNLQWGVFPLRIDLS+DIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 581
           GIE+VKSKGLVKQGD VL+VSEISPARA SMASQSIQLK+IV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

[Close]
BLAST of Carg08103 vs. TrEMBL
Match: tr|A0A0A0KSY4|A0A0A0KSY4_CUCSA (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G000560 PE=3 SV=1)

HSP 1 Score: 941.0 bits (2431), Expect = 1.2e-270
Identity = 510/582 (87.63%), Postives = 535/582 (91.92%), Query Frame = 0 

Query: 1   MAEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIG-RTVWCDRGRMKGSVVTGVKAAMQV 60
           MAEV+AMMIRA CNF+ +  VLDVKR   R+ V+G RTVWC R RMKG VV GVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVVNGVKATMQV 60

Query: 61  DLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLAL 120
           DLED+ERLKRL+GLEATLGLD+VAERELKEKGFLGMRKTK VCT+GPAC  I DLEKLAL
Sbjct: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120

Query: 121 EGMNIARLNMCHNKKEWHYDVIRKIKKLN-EKGFCISVMIDTEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHN  EWH D+I+KIKKLN EKGFCISVMID EGSQIHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180

Query: 181 EDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCK 240
           EDGS WLFTA+KFEGSRPFTVQASYEGFSEGI+VGDSIVIDGGMA+FEV+EKIGNDLSC+
Sbjct: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSAXX 300
           CTDPGLFLPRAKCSFWRDGRLVMR HESPTLSSKDWSDIEFGISEGVDFIALSFVNSA  
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARGDLGVEIPLEQIPAV 360
                      XXXXXXXXXXXXXXXXXXXXXXX  ASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLSTKXXXXXXXXXXXXXXXXXXXXXXXXXASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGA 420
           QEEIT VCRELNKPVI+ASQLLESMVEYPTPTRAEVADVSE VRQYADALMLSGESAIG+
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVD 480
           YGQKALSVLQ+ASSRMELWSREEN  +FLPQHQLGVSLHDRIAEEICNSAAELAN+LSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISR 540
           AIFVLT QGHMASLLSRNRP PPIFALTDDDSTRMALNLQWGVFPLRIDLS+DIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 581
           GIE+VKSKGLVKQGD VL+VSEISPARAASMASQSIQLK+IV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 582

[Close]
BLAST of Carg08103 vs. TrEMBL
Match: tr|A0A2N9F1S9|A0A2N9F1S9_FAGSY (Pyruvate kinase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS8626 PE=3 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 1.8e-221
Identity = 424/574 (73.87%), Postives = 480/574 (83.62%), Query Frame = 0 

Query: 12  CCNFVAKTSVLDVKRSDSRLSVIGRTVW-----CDRGRMKGSVVTGVKAAMQVDLEDDER 71
           C   + K   L++K       VIGR V+     C+ G++KG +   V+AA+QV +E  E 
Sbjct: 11  CGRIIGKNPCLELKN-----RVIGRPVFAPGFCCNGGKLKGKIGIRVRAAVQVGVEKSEG 70

Query: 72  LKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALEGMNIAR 131
           L  L GL   LG DVV+E EL+EKGFLGMRKTK VCTIGPACC++EDLEKLAL GMN+AR
Sbjct: 71  LIDLKGLSGVLGFDVVSEGELREKGFLGMRKTKLVCTIGPACCSLEDLEKLALGGMNVAR 130

Query: 132 LNMCHNKKEWHYDVIRKIKKLNE-KGFCISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWL 191
           LNMCHN +EWH DVIRKIK+LNE KGFC+SVMIDTEGSQIHVVDHG+PSSVK EDGS WL
Sbjct: 131 LNMCHNGREWHRDVIRKIKRLNEDKGFCVSVMIDTEGSQIHVVDHGSPSSVKAEDGSIWL 190

Query: 192 FTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCTDPGLF 251
           FT +KFEGSRPFTVQA+YEGFSEGI+VGD +VIDGGMA FEV+EKIG DL CKCTDPGLF
Sbjct: 191 FTTQKFEGSRPFTVQANYEGFSEGIEVGDELVIDGGMAGFEVIEKIGGDLRCKCTDPGLF 250

Query: 252 LPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSAXXXXXXXXX 311
           LPRAK SFWRDG+LV RKHE PTLS+KDW+DIEFG+SEG+DFIA+SFVN +         
Sbjct: 251 LPRAKLSFWRDGKLVRRKHELPTLSTKDWADIEFGVSEGIDFIAMSFVNDSDSVKHLKNY 310

Query: 312 XXXXXXXXXXXXXXXXXXXXXXXXXXXIKASDGIMVARGDLGVEIPLEQIPAVQEEITSV 371
           XXXXXXXXXXXXXXXXXXXXXXXXXXX  ASDGIMVARGDLGVEIPLEQIP VQE I +V
Sbjct: 311 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXASDGIMVARGDLGVEIPLEQIPTVQEVIINV 370

Query: 372 CRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYGQKALS 431
           CR+LNKPVIVASQLLESMVEYPTPTRAEVADVSE +RQYADALMLSGESAIG YGQKALS
Sbjct: 371 CRQLNKPVIVASQLLESMVEYPTPTRAEVADVSEAIRQYADALMLSGESAIGLYGQKALS 430

Query: 432 VLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAIFVLTK 491
           VL++ SSR+E W REE+ +  L QHQLGVSL DRIAEEICNSAAE+ANKL VDAIFV TK
Sbjct: 431 VLRMTSSRIETWGREESQQSLLHQHQLGVSLPDRIAEEICNSAAEMANKLGVDAIFVYTK 490

Query: 492 QGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGIELVKS 551
            GHMASLLSRNRP+PPIFA TD+DSTR ALNLQWGV PL +DLSDD+EANIS+ I+L+K 
Sbjct: 491 HGHMASLLSRNRPNPPIFAFTDNDSTRKALNLQWGVIPLLVDLSDDVEANISKTIDLIKR 550

Query: 552 KGLVKQGDFVLMVSEISPARAASMASQSIQLKSI 580
            G++KQGD VL+VS+++P  A  MA QS Q+K+I
Sbjct: 551 TGMLKQGDAVLVVSDVTPTLATPMAFQSFQVKTI 579

[Close]
BLAST of Carg08103 vs. TrEMBL
Match: tr|A0A2P4KD79|A0A2P4KD79_QUESU (Pyruvate kinase OS=Quercus suber OX=58331 GN=CFP56_05359 PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 1.7e-219
Identity = 422/566 (74.56%), Postives = 484/566 (85.51%), Query Frame = 0 

Query: 16  VAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGSVVTGVKAAMQVDLEDDERLKR-LDGLE 75
           + K   LD+K       V  R +  + G++KG +  GV+AA+QV++E+ E L+R L GL 
Sbjct: 15  IGKNPCLDLKNRVLGRPVFARGICFNGGKVKGKIGVGVRAAVQVNVEETEGLQRELKGLR 74

Query: 76  ATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKK 135
             LG DVV+E EL+EKGFLGMRKTK VCTIGPACC++EDLEKLAL GMN+ARLNMCHN +
Sbjct: 75  GVLGFDVVSEGELREKGFLGMRKTKLVCTIGPACCSLEDLEKLALGGMNVARLNMCHNGR 134

Query: 136 EWHYDVIRKIKKLN-EKGFCISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEG 195
           EWH DVIRKIK+LN EKGFC+SVMIDTEGSQI VVDHGAPSSVKVEDGS WLFTAEKF G
Sbjct: 135 EWHRDVIRKIKRLNEEKGFCVSVMIDTEGSQIQVVDHGAPSSVKVEDGSIWLFTAEKFAG 194

Query: 196 SRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCSF 255
           SRPFTVQA+YEGFSEGI+VGD +VIDGGMA FEV+EKIG+DL CKCTDPGLFLPRAK SF
Sbjct: 195 SRPFTVQANYEGFSEGIEVGDELVIDGGMAGFEVIEKIGSDLRCKCTDPGLFLPRAKLSF 254

Query: 256 WRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSAXXXXXXXXXXXXXXXXX 315
           WRDG+LV R +E PTLS+KDW+DIEFG+SEG+DFIA+SFVN +       XXXXXXXXXX
Sbjct: 255 WRDGKLVGRNYELPTLSAKDWADIEFGVSEGIDFIAMSFVNDSDSVKHLKXXXXXXXXXX 314

Query: 316 XXXXXXXXXXXXXXXXXXXIKASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPV 375
           XXXXXXXXXXXXXXXXXXX++ASDGIMVARGDLGVEIPLEQIP+VQEEI +VCR+LNKPV
Sbjct: 315 XXXXXXXXXXXXXXXXXXXVEASDGIMVARGDLGVEIPLEQIPSVQEEIINVCRQLNKPV 374

Query: 376 IVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSR 435
           IVASQLLESMVEYPTPTRAEVADVSE VRQYADALMLSGESAIG  GQKALSVL++ SSR
Sbjct: 375 IVASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGLNGQKALSVLRMTSSR 434

Query: 436 MELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLL 495
           +E W REE+ +  LPQHQLGVSL DRIAE+ICN AAE+ANKL VDAIFV TK GHMASLL
Sbjct: 435 IETWGREESQQTLLPQHQLGVSLPDRIAEQICNCAAEMANKLGVDAIFVYTKHGHMASLL 494

Query: 496 SRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGD 555
           SRNRP+PPIFA T+DD+TR ALNLQWGV  L +DLSDD+EANIS+ I L+K  G+++QGD
Sbjct: 495 SRNRPNPPIFAFTNDDNTRKALNLQWGVTSLLVDLSDDMEANISKTINLIKRTGMLEQGD 554

Query: 556 FVLMVSEISPARAASMASQSIQLKSI 580
            VL+VS+++P  A  MA QSIQ+K+I
Sbjct: 555 AVLVVSDVTPTLATPMAFQSIQVKTI 580

The following BLAST results are available for this feature:
BLAST of Carg08103 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022942487.13.0e-30098.79plastidial pyruvate kinase 1, chloroplastic-like isoform X1 [Cucurbita moschata][more]
XP_022975726.11.9e-29497.24plastidial pyruvate kinase 1, chloroplastic-like isoform X1 [Cucurbita maxima][more]
XP_023515955.11.2e-29396.90plastidial pyruvate kinase 1, chloroplastic-like isoform X1 [Cucurbita pepo subs... [more]
XP_022942488.17.3e-27598.88plastidial pyruvate kinase 1, chloroplastic-like isoform X2 [Cucurbita moschata][more]
XP_023515961.12.9e-27197.39plastidial pyruvate kinase 1, chloroplastic-like isoform X2 [Cucurbita pepo subs... [more]
BLAST of Carg08103 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G22960.11.2e-15456.06Pyruvate kinase family protein[more]
AT5G52920.12.1e-7434.98plastidic pyruvate kinase beta subunit 1[more]
AT1G32440.19.8e-7235.45plastidial pyruvate kinase 3[more]
AT3G04050.13.1e-4931.27Pyruvate kinase family protein[more]
AT5G63680.15.8e-4829.15Pyruvate kinase family protein[more]
BLAST of Carg08103 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9LIK0|PKP1_ARATH2.2e-15356.06Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P... [more]
sp|Q43117|KPYA_RICCO8.3e-15354.79Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1[more]
sp|Q40545|KPYA_TOBAC1.4e-14753.56Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
sp|Q40546|KPYG_TOBAC1.4e-7536.21Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
sp|Q9FLW9|PKP2_ARATH3.8e-7334.98Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1[more]
BLAST of Carg08103 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3BYK3|A0A1S3BYK3_CUCME2.5e-27183.68Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103494833 PE=3 SV=1[more]
tr|E5GBH7|E5GBH7_CUCME2.5e-27183.68Pyruvate kinase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
tr|A0A0A0KSY4|A0A0A0KSY4_CUCSA1.2e-27087.63Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G000560 PE=3 SV=1[more]
tr|A0A2N9F1S9|A0A2N9F1S9_FAGSY1.8e-22173.87Pyruvate kinase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS8626 PE=3 SV=1[more]
tr|A0A2P4KD79|A0A2P4KD79_QUESU1.7e-21974.56Pyruvate kinase OS=Quercus suber OX=58331 GN=CFP56_05359 PE=3 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0000287magnesium ion binding
GO:0030955potassium ion binding
GO:0004743pyruvate kinase activity
Vocabulary: Biological Process
TermDefinition
GO:0006096glycolytic process
Vocabulary: INTERPRO
TermDefinition
IPR011037Pyrv_Knase-like_insert_dom_sf
IPR015813Pyrv/PenolPyrv_Kinase-like_dom
IPR018209Pyrv_Knase_AS
IPR015795Pyrv_Knase_C
IPR015793Pyrv_Knase_brl
IPR036918Pyrv_Knase_C_sf
IPR015806Pyrv_Knase_insert_dom_sf
IPR001697Pyr_Knase
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015976 carbon utilization
biological_process GO:0006094 gluconeogenesis
biological_process GO:0006096 glycolytic process
biological_process GO:0006144 purine nucleobase metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0003824 catalytic activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08103-RACarg08103-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 152..168
score: 27.42
coord: 285..299
score: 47.28
coord: 343..367
score: 48.92
coord: 393..411
score: 49.07
coord: 368..392
score: 58.28
coord: 412..428
score: 35.38
coord: 316..342
score: 50.88
IPR001697Pyruvate kinaseTIGRFAMTIGR01064TIGR01064coord: 96..565
e-value: 2.5E-121
score: 403.4
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 55..579
NoneNo IPR availableGENE3DG3DSA:3.20.20.60coord: 96..163
e-value: 3.5E-145
score: 486.1
coord: 267..443
e-value: 3.5E-145
score: 486.1
NoneNo IPR availablePANTHERPTHR11817:SF21SUBFAMILY NOT NAMEDcoord: 55..579
IPR015806Pyruvate kinase, insert domain superfamilyGENE3DG3DSA:2.40.33.10coord: 164..266
e-value: 3.5E-145
score: 486.1
IPR036918Pyruvate kinase, C-terminal domain superfamilyGENE3DG3DSA:3.40.1380.20coord: 444..555
e-value: 3.5E-145
score: 486.1
IPR036918Pyruvate kinase, C-terminal domain superfamilySUPERFAMILYSSF52935PK C-terminal domain-likecoord: 458..561
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 95..430
e-value: 1.0E-84
score: 284.3
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 462..562
e-value: 4.4E-21
score: 75.2
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 314..326
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILYSSF51621Phosphoenolpyruvate/pyruvate domaincoord: 90..168
coord: 266..435
IPR011037Pyruvate kinase-like, insert domain superfamilySUPERFAMILYSSF50800PK beta-barrel domain-likecoord: 165..252

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Carg08103Cla006459Watermelon (97103) v1carwmB0366
Carg08103Csa4G000560Cucumber (Chinese Long) v2carcuB0402
Carg08103MELO3C018027Melon (DHL92) v3.5.1carmeB0383
Carg08103ClCG10G009990Watermelon (Charleston Gray)carwcgB0365
Carg08103CSPI04G00070Wild cucumber (PI 183967)carcpiB0407
Carg08103Cucsa.298530Cucumber (Gy14) v1carcgyB0439
Carg08103CmaCh04G004280Cucurbita maxima (Rimu)carcmaB0529
Carg08103CmaCh16G003030Cucurbita maxima (Rimu)carcmaB0526
Carg08103CmoCh16G003210Cucurbita moschata (Rifu)carcmoB0509
Carg08103CmoCh04G004600Cucurbita moschata (Rifu)carcmoB0514
Carg08103Lsi03G010040Bottle gourd (USVL1VR-Ls)carlsiB341
Carg08103Cp4.1LG01g02840Cucurbita pepo (Zucchini)carcpeB0527
Carg08103Cp4.1LG14g04760Cucurbita pepo (Zucchini)carcpeB0523
Carg08103MELO3C018027.2Melon (DHL92) v3.6.1carmedB0387
Carg08103CsaV3_4G000040Cucumber (Chinese Long) v3carcucB0406
Carg08103Cla97C10G193010Watermelon (97103) v2carwmbB0391
Carg08103Bhi11G000910Wax gourdcarwgoB0456
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg08103Carg19702Silver-seed gourdcarcarB324
The following block(s) are covering this gene:
GeneOrganismBlock
Carg08103Silver-seed gourdcarcarB401
Carg08103Silver-seed gourdcarcarB402
Carg08103Silver-seed gourdcarcarB404
Carg08103Cucumber (Gy14) v2carcgybB0383
Carg08103Cucumber (Gy14) v2carcgybB0388
Carg08103Cucumber (Gy14) v2carcgybB0389
Carg08103Cucumber (Gy14) v1carcgyB0433
Carg08103Cucurbita maxima (Rimu)carcmaB0528
Carg08103Cucurbita maxima (Rimu)carcmaB0525
Carg08103Cucurbita moschata (Rifu)carcmoB0508
Carg08103Cucurbita moschata (Rifu)carcmoB0516
Carg08103Cucurbita pepo (Zucchini)carcpeB0526
Carg08103Cucurbita pepo (Zucchini)carcpeB0529
Carg08103Cucumber (Chinese Long) v2carcuB0409
Carg08103Cucumber (Chinese Long) v2carcuB0410
Carg08103Bottle gourd (USVL1VR-Ls)carlsiB340
Carg08103Melon (DHL92) v3.5.1carmeB0386
Carg08103Melon (DHL92) v3.6.1carmedB0390
Carg08103Watermelon (Charleston Gray)carwcgB0368
Carg08103Watermelon (97103) v1carwmB0375
Carg08103Watermelon (97103) v2carwmbB0394