CmaCh08G005700 (gene) Cucurbita maxima (Rimu)

NameCmaCh08G005700
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
Description4-coumarate:CoA ligase
LocationCma_Chr08 : 3253231 .. 3255245 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GACCACGAAACCAAATTTTTATAAAAAAATCCGATTTATTTTTCTCACCAACCAAACACTAATTTCTAAAATTTTCTCGTCTCTATAAAATCTCTGCATTTCTCTTCATCCTTCTTCACACCAAGCCCACCTCTGTTTTTTTCTCTGCTCTCTGTATATCCTCAATTCTGAGCTTCAACCTTCAACAATGGCCGCTGAAATTAACCAAACAAAGGAATTCATTTTCCGTTCAAAGCTTCCCGATATTCACATTCCCAATCATCTTCCACTCCATGAATATATTTTTCAAAACCTCCCCAATTACGGCTCCCGCCCCTGTTTGATTAACGGCGCCACCGGCCACGTTTACTCATACGACGACGTTCAGTTAACAGCCCGTCGTGTCGCCAGTGGACTTCACAATCTCGGGATTAAGAAGGGTGACGTCGTTATGAATTTACTTCCCAACTCGCCGGAATTTGTTTTCACGTTTCTCGGAGCGTCGTATCGCGGTGCTATCATGACGGCGGCGAATCCGTTTTACACGGCGGTGGAAATTGCCAAACAGGCGAAAGCTGCTAATGCGAAATTGATTGTCACCATGGCTTGCTTTTATGATCGGGTTAAGGATTTGGCTGAAAATGGGGTGAAAATCGTGTGTGTTGATTATGCTGTTGAGGGTTGTTTGCATTACTCTGTTTTGAGCGGCGCGGATGAATCCCATGTGCCGTTTGTGGATTTCACCGCCGATGATGTGGTGGCGCTGCCGTATTCCTCCGGCACCACGGGTTTGCCAAAGGGTGTTATGTTGACTCATAAAGGGTTGATCACAAGCGTTGCTCAACAAATCGACGGCCAAAATCCGAATCTGTATTACAAACGCAATGACGTTATCCTCTGTGTTTTGCCGTTTTTTCACATCTATTCACTCAATTCGATTTTGCTGTGTGGATTACGCGCCGGTGCTGCGATTTTGATAATGCAAAAATTCGAAATCGTTTCGCTTTTGGAATTAGTTGAGAAATATAAAGCTTCAATCGTGCCGATTGTGCCGCCGATTCTTTTAGCCATTGCTAAGTCATCGGAATTCGAGAAATACGACATGTCGTCAGTGAGGGTATTGAAATCCGGTGGAGCACCGTTGGGGAAAAAATTGGAAGACGCTGTGAGGGAGAAGTTTCCAACGGCGATTCTCGGGCAAGGATATGGAATGACCGAAGCCGGTCCGGTTCTGACCATGAGTTTAGCGTTTGCGAAAGAACCGTTTCCGGTGAAACCCGGCGCTTGTGGGACGGTGGTTCGAAATGCAGAGATGAAGATTATTGATCCAGAAACCGGCGTTTCGTTGCCGGCGAATTCCGCCGGCGAGATTTGTATCAGAGGGGATCAGATCATGAAGGGATACTTAAACGATTTGGAGTCTACGAAGAGGACTATTGACAAAGAAGGATGGCTCCACACCGGCGACATTGGATTCATCGACGACGACGACGAGCTCTTCATCGTCGACCGGCTTAAAGAACTGATAAAATTCAAGACGTTTCAGGTGGCGCCGGCGGAGCTGGAGGCTCTTCTAATCGCTCATCCCAAACTATCCGATGCAGCGGTGATTGGGTAAGCCATAATGAATTTTGTCGTTTATAAATAAAAGAAATCATTTTTTTCCTAATTCATTTTTTTTTTTGTTCTGAACAGTATGCCGGACGAGCAAGCCGGAGAATTGCCGGTGGCATTTGTGGTGAATGCGAACGGCGGCGAAATCACAGAAGAGGACGTAAAGCAATTCATATCAGAACAAGTGGTGTTCTACAAAAGGCTAAAACGAGTGTTTTTCGTCAACGCTATCCCAAAGGCTCCGTCGGGCAAAATCCTCAGAAAAGAACTCCGATCACAATTGGCCGCCGGTGTTTACAATTAAACGCCAAATAATTTATTATTTTCCATTTATGTTTCGTTTGTGAATTCAAATTATGTATTTCGGATTTTTAAGTATATTTTTTAAAAATAATAAACCAAATATCTATATTGCTTTATTG

mRNA sequence

GACCACGAAACCAAATTTTTATAAAAAAATCCGATTTATTTTTCTCACCAACCAAACACTAATTTCTAAAATTTTCTCGTCTCTATAAAATCTCTGCATTTCTCTTCATCCTTCTTCACACCAAGCCCACCTCTGTTTTTTTCTCTGCTCTCTGTATATCCTCAATTCTGAGCTTCAACCTTCAACAATGGCCGCTGAAATTAACCAAACAAAGGAATTCATTTTCCGTTCAAAGCTTCCCGATATTCACATTCCCAATCATCTTCCACTCCATGAATATATTTTTCAAAACCTCCCCAATTACGGCTCCCGCCCCTGTTTGATTAACGGCGCCACCGGCCACGTTTACTCATACGACGACGTTCAGTTAACAGCCCGTCGTGTCGCCAGTGGACTTCACAATCTCGGGATTAAGAAGGGTGACGTCGTTATGAATTTACTTCCCAACTCGCCGGAATTTGTTTTCACGTTTCTCGGAGCGTCGTATCGCGGTGCTATCATGACGGCGGCGAATCCGTTTTACACGGCGGTGGAAATTGCCAAACAGGCGAAAGCTGCTAATGCGAAATTGATTGTCACCATGGCTTGCTTTTATGATCGGGTTAAGGATTTGGCTGAAAATGGGGTGAAAATCGTGTGTGTTGATTATGCTGTTGAGGGTTGTTTGCATTACTCTGTTTTGAGCGGCGCGGATGAATCCCATGTGCCGTTTGTGGATTTCACCGCCGATGATGTGGTGGCGCTGCCGTATTCCTCCGGCACCACGGGTTTGCCAAAGGGTGTTATGTTGACTCATAAAGGGTTGATCACAAGCGTTGCTCAACAAATCGACGGCCAAAATCCGAATCTGTATTACAAACGCAATGACGTTATCCTCTGTGTTTTGCCGTTTTTTCACATCTATTCACTCAATTCGATTTTGCTGTGTGGATTACGCGCCGGTGCTGCGATTTTGATAATGCAAAAATTCGAAATCGTTTCGCTTTTGGAATTAGTTGAGAAATATAAAGCTTCAATCGTGCCGATTGTGCCGCCGATTCTTTTAGCCATTGCTAAGTCATCGGAATTCGAGAAATACGACATGTCGTCAGTGAGGGTATTGAAATCCGGTGGAGCACCGTTGGGGAAAAAATTGGAAGACGCTGTGAGGGAGAAGTTTCCAACGGCGATTCTCGGGCAAGGATATGGAATGACCGAAGCCGGTCCGGTTCTGACCATGAGTTTAGCGTTTGCGAAAGAACCGTTTCCGGTGAAACCCGGCGCTTGTGGGACGGTGGTTCGAAATGCAGAGATGAAGATTATTGATCCAGAAACCGGCGTTTCGTTGCCGGCGAATTCCGCCGGCGAGATTTGTATCAGAGGGGATCAGATCATGAAGGGATACTTAAACGATTTGGAGTCTACGAAGAGGACTATTGACAAAGAAGGATGGCTCCACACCGGCGACATTGGATTCATCGACGACGACGACGAGCTCTTCATCGTCGACCGGCTTAAAGAACTGATAAAATTCAAGACGTTTCAGGTGGCGCCGGCGGAGCTGGAGGCTCTTCTAATCGCTCATCCCAAACTATCCGATGCAGCGGTGATTGGTATGCCGGACGAGCAAGCCGGAGAATTGCCGGTGGCATTTGTGGTGAATGCGAACGGCGGCGAAATCACAGAAGAGGACGTAAAGCAATTCATATCAGAACAAGTGGTGTTCTACAAAAGGCTAAAACGAGTGTTTTTCGTCAACGCTATCCCAAAGGCTCCGTCGGGCAAAATCCTCAGAAAAGAACTCCGATCACAATTGGCCGCCGGTGTTTACAATTAAACGCCAAATAATTTATTATTTTCCATTTATGTTTCGTTTGTGAATTCAAATTATGTATTTCGGATTTTTAAGTATATTTTTTAAAAATAATAAACCAAATATCTATATTGCTTTATTG

Coding sequence (CDS)

ATGGCCGCTGAAATTAACCAAACAAAGGAATTCATTTTCCGTTCAAAGCTTCCCGATATTCACATTCCCAATCATCTTCCACTCCATGAATATATTTTTCAAAACCTCCCCAATTACGGCTCCCGCCCCTGTTTGATTAACGGCGCCACCGGCCACGTTTACTCATACGACGACGTTCAGTTAACAGCCCGTCGTGTCGCCAGTGGACTTCACAATCTCGGGATTAAGAAGGGTGACGTCGTTATGAATTTACTTCCCAACTCGCCGGAATTTGTTTTCACGTTTCTCGGAGCGTCGTATCGCGGTGCTATCATGACGGCGGCGAATCCGTTTTACACGGCGGTGGAAATTGCCAAACAGGCGAAAGCTGCTAATGCGAAATTGATTGTCACCATGGCTTGCTTTTATGATCGGGTTAAGGATTTGGCTGAAAATGGGGTGAAAATCGTGTGTGTTGATTATGCTGTTGAGGGTTGTTTGCATTACTCTGTTTTGAGCGGCGCGGATGAATCCCATGTGCCGTTTGTGGATTTCACCGCCGATGATGTGGTGGCGCTGCCGTATTCCTCCGGCACCACGGGTTTGCCAAAGGGTGTTATGTTGACTCATAAAGGGTTGATCACAAGCGTTGCTCAACAAATCGACGGCCAAAATCCGAATCTGTATTACAAACGCAATGACGTTATCCTCTGTGTTTTGCCGTTTTTTCACATCTATTCACTCAATTCGATTTTGCTGTGTGGATTACGCGCCGGTGCTGCGATTTTGATAATGCAAAAATTCGAAATCGTTTCGCTTTTGGAATTAGTTGAGAAATATAAAGCTTCAATCGTGCCGATTGTGCCGCCGATTCTTTTAGCCATTGCTAAGTCATCGGAATTCGAGAAATACGACATGTCGTCAGTGAGGGTATTGAAATCCGGTGGAGCACCGTTGGGGAAAAAATTGGAAGACGCTGTGAGGGAGAAGTTTCCAACGGCGATTCTCGGGCAAGGATATGGAATGACCGAAGCCGGTCCGGTTCTGACCATGAGTTTAGCGTTTGCGAAAGAACCGTTTCCGGTGAAACCCGGCGCTTGTGGGACGGTGGTTCGAAATGCAGAGATGAAGATTATTGATCCAGAAACCGGCGTTTCGTTGCCGGCGAATTCCGCCGGCGAGATTTGTATCAGAGGGGATCAGATCATGAAGGGATACTTAAACGATTTGGAGTCTACGAAGAGGACTATTGACAAAGAAGGATGGCTCCACACCGGCGACATTGGATTCATCGACGACGACGACGAGCTCTTCATCGTCGACCGGCTTAAAGAACTGATAAAATTCAAGACGTTTCAGGTGGCGCCGGCGGAGCTGGAGGCTCTTCTAATCGCTCATCCCAAACTATCCGATGCAGCGGTGATTGGTATGCCGGACGAGCAAGCCGGAGAATTGCCGGTGGCATTTGTGGTGAATGCGAACGGCGGCGAAATCACAGAAGAGGACGTAAAGCAATTCATATCAGAACAAGTGGTGTTCTACAAAAGGCTAAAACGAGTGTTTTTCGTCAACGCTATCCCAAAGGCTCCGTCGGGCAAAATCCTCAGAAAAGAACTCCGATCACAATTGGCCGCCGGTGTTTACAATTAA

Protein sequence

MAAEINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQLTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFTADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYSLNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDMSSVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGACGTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPVAFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGVYN
BLAST of CmaCh08G005700 vs. Swiss-Prot
Match: 4CL2_TOBAC (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum GN=4CL2 PE=1 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 2.5e-228
Identity = 387/534 (72.47%), Postives = 454/534 (85.02%), Query Frame = 1

Query: 10  EFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQLTARRVASG 69
           + IFRSKLPDI+IPNHLPLH Y F+N+  + SRPCLINGA   +Y+Y DV+L +R+VA+G
Sbjct: 9   DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 70  LHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLI 129
           LH  GI+  D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA++AK+I
Sbjct: 69  LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 130 VTMACFYDRVKDLA-ENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFTADDVVALPY 189
           VT AC  ++VKD A EN VKI+C+D A EGCLH+SVL+ A+E  +P V+   DDVVALPY
Sbjct: 129 VTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVVALPY 188

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY    DV+LCVLP FHIYSLNS+LLCG
Sbjct: 189 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLLCG 248

Query: 250 LRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDMSSVRVLKSG 309
           LR GAAILIMQKF+IVS LEL+++YK +I P VPPI+LAIAKS   + YD+SSVR + SG
Sbjct: 249 LRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRTVMSG 308

Query: 310 GAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGACGTVVRNAE 369
            APLGK+LED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K GACGTVVRNAE
Sbjct: 309 AAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAE 368

Query: 370 MKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGFIDDDD 429
           MKI+DP+TG SLP N +GEICIRGDQIMKGYLND E+T RTIDKEGWL+TGDIG+IDDDD
Sbjct: 369 MKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDDDD 428

Query: 430 ELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPVAFVVNANG 489
           ELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+ M DEQAGE+PVAFVV +NG
Sbjct: 429 ELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNG 488

Query: 490 GEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGVYN 543
             ITE++VK FIS+QV+FYKR+KRVFFV+AIPK+PSGKILRK+LR++LAAG+ N
Sbjct: 489 STITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542

BLAST of CmaCh08G005700 vs. Swiss-Prot
Match: 4CL1_TOBAC (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum GN=4CL1 PE=2 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 4.7e-227
Identity = 384/540 (71.11%), Postives = 450/540 (83.33%), Query Frame = 1

Query: 4   EINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQLTA 63
           E  Q+ + IFRSKLPDI+IP HLPLH Y F+N+  + SRPCLINGA   +Y+Y +V+LT 
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 64  RRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKA 123
           R+VA GL+ LGI++ D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 124 ANAKLIVTMACFYDRVKDLA-ENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFTADD 183
           ++AK+I+T +CF  +VKD A EN VK++C+D A EGCLH+S L+ +DE  +P V    DD
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDD 187

Query: 184 VVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYSLN 243
           VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY    DV++CVLP FHIYSLN
Sbjct: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 247

Query: 244 SILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDMSSV 303
           SILLCGLR GAAILIMQKF+I   LEL++KYK SI P VPPI+LAIAKS   + YD+SSV
Sbjct: 248 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 307

Query: 304 RVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGACGT 363
           R + SG APLGK+LEDAVR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K GACGT
Sbjct: 308 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 367

Query: 364 VVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIG 423
           VVRNAEMKI+DP+TG SLP N  GEICIRGDQIMKGYLND E+T RTIDKEGWLHTGDIG
Sbjct: 368 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 427

Query: 424 FIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPVAF 483
           FID+DDELFIVDRLKELIK+K FQVAPAE+EALL+ HP +SDAAV+ M DEQAGE+PVAF
Sbjct: 428 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 487

Query: 484 VVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGVYN 543
           VV +NG  ITE++VK FIS+QV+FYKR+KRVFFV  +PK+PSGKILRK+LR++LAAGV N
Sbjct: 488 VVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

BLAST of CmaCh08G005700 vs. Swiss-Prot
Match: 4CL_VANPL (4-coumarate--CoA ligase OS=Vanilla planifolia GN=4CL PE=3 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 3.0e-226
Identity = 390/547 (71.30%), Postives = 456/547 (83.36%), Query Frame = 1

Query: 1   MAAEI---NQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYD 60
           MAA +    Q K+ IFRSKLPDI+IP +LPLH Y F+N+  + SRPCLINGAT  +++Y 
Sbjct: 1   MAAAVAIEEQKKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYA 60

Query: 61  DVQLTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEI 120
           DV+L +RRV SGL  LGIK+GD +M LLPNSPEFVF FLGAS+ G+I T ANPF+T+ E+
Sbjct: 61  DVELISRRVGSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEV 120

Query: 121 AKQAKAANAKLIVTMACFYDRVKDLA-ENGVKIVCVDYAVEG-----CLHYSVLSGADES 180
            KQAKA+NAKLI+T  C+ D+VKD A ENGVKI+ +D           LH+S L+GADE+
Sbjct: 121 IKQAKASNAKLIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADEN 180

Query: 181 HVPFVDFTADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILC 240
            +P V+ + D VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY   +DV+LC
Sbjct: 181 EMPKVEISPDGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLC 240

Query: 241 VLPFFHIYSLNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKS 300
           VLP FHIYSLNS+LLCGLRAG+ ILIMQKFEIV  LEL++KYK +I P VPPI+LAIAKS
Sbjct: 241 VLPLFHIYSLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKS 300

Query: 301 SEFEKYDMSSVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKE 360
           +  + YD+SSVR + SG APLGK+LEDAVR KFP A LGQGYGMTEAGPVL M LAFAKE
Sbjct: 301 TVVDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKE 360

Query: 361 PFPVKPGACGTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTID 420
           PF +K GACGTVVRNAEMKI+DPETG SLP N  GEICIRGDQIMKGYLND E+T RTID
Sbjct: 361 PFDIKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTID 420

Query: 421 KEGWLHTGDIGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMP 480
           KEGWLHTGDIG+IDDDDELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+ M 
Sbjct: 421 KEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMK 480

Query: 481 DEQAGELPVAFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKE 539
           DE AGE+PVAFVV +NG  ITE+++KQFIS+QV+FYKR+ RVFFV AIPKAPSGKILRK+
Sbjct: 481 DEAAGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKD 540

BLAST of CmaCh08G005700 vs. Swiss-Prot
Match: 4CL2_SOLTU (4-coumarate--CoA ligase 2 OS=Solanum tuberosum GN=4CL2 PE=3 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 6.3e-224
Identity = 380/543 (69.98%), Postives = 448/543 (82.50%), Query Frame = 1

Query: 1   MAAEINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQ 60
           M  E  Q+ + IFRSKLPDI+IP HLPLH Y F+NL  + SRPCLI+GA   +Y+Y +V+
Sbjct: 3   MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+R+VA GL+ LGI++ D +M LLPN PEFVF F+GASY GAI T ANP +T  E+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFT 180
           AKA++AK+++T ACF  +VKD A EN +K++CVD A EGC+H+S L  +DE  +P V   
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 ADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIY 240
            DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY   +DV++CVLP FHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDM 300
           SLNS+LLC LR GAAILIMQKF+I   LEL+ K+K +I P VPPI+LAIAKS     YD+
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDL 302

Query: 301 SSVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGA 360
           SSVR + SG APLGK+LEDAVR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKI+DP+TG SLP N  GEICIRGDQIMKGYLND E+T RTI+KEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DIGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELP 480
           DIGFIDDDDELFIVDRLKELIK+K FQVAPAELEALLI HP +SDAAV+ M DEQAGE+P
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAG 540
           VAFVV +NG  ITE++VK FIS+QV+FYKR+KRVFFV  +PK+PSGKILRK+LR++LAAG
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 VYN 543
           + N
Sbjct: 543 ISN 545

BLAST of CmaCh08G005700 vs. Swiss-Prot
Match: 4CL1_SOLTU (4-coumarate--CoA ligase 1 OS=Solanum tuberosum GN=4CL1 PE=3 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 8.3e-224
Identity = 379/543 (69.80%), Postives = 448/543 (82.50%), Query Frame = 1

Query: 1   MAAEINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQ 60
           M  E  Q+ + IFRSKLPDI+IP HLPLH Y F+NL  + SRPCLI+GA   +Y+Y +V+
Sbjct: 3   MDTETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+R+VA GL+ LGI++ D +M LLPN PEFVF F+GASY GAI T ANP +T  E+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFT 180
           AKA++AK+++T ACF  +VKD A EN +K++CVD   EGC+H+S L  +DE  +P V   
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSVPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 ADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIY 240
            DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY   +DV++CVLP FHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDM 300
           SLNS+LLC LR GAAILIMQKF+I   LEL+ K+K +I P VPPI+LAIAKS   + YD+
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDL 302

Query: 301 SSVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGA 360
           SSVR + SG APLGK+LEDAVR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKI+DP+TG SLP N  GEICIRGDQIMKGYLND E+T RTI+KEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DIGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELP 480
           DIGFIDDDDELFIVDRLKELIK+K FQVAPAELEALLI HP +SDAAV+ M DEQAGE+P
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAG 540
           VAFVV +NG  ITE++VK FIS+QV+FYKR+KRVFFV  +PK+PSGKILRK+LR++LAAG
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 VYN 543
           + N
Sbjct: 543 ISN 545

BLAST of CmaCh08G005700 vs. TrEMBL
Match: A0A0A0K8H9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430240 PE=4 SV=1)

HSP 1 Score: 967.2 bits (2499), Expect = 8.2e-279
Identity = 478/542 (88.19%), Postives = 516/542 (95.20%), Query Frame = 1

Query: 1   MAAEINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQ 60
           MA E N+T EFIFRSKLPDIHIPNHLPLH+Y+FQNL  + SRPCLINGATG VY+Y DVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVA+GLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFTA 180
           AKAANAKLIVTMACFYDRVKDLAENGV+IVCVD+AVEGCLH+SVLSGADES  P VDF++
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYY RNDVILCVLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDMS 300
           LNSILLCGLR GAAI+IMQKF+IV+LL+L+EK++ SI+PIVPPI LAIAKS EFEKYD+S
Sbjct: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGAC 360
           SVRVLKSGGAPLGK+LEDAVREKFPTAILGQGYGMTEAGPVL+MSLAFAKEPF VK GAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKI+D ETG SLPANS+GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480
           IGF+DDD+ELFIVDRLKELIKFK FQVAPAELEALLI HPKLSDAAVIGMPD +AGE+PV
Sbjct: 421 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540
           AFV+ ANGG I+EE+VKQFI++QVVFYKRLKRVFFVNAIPKAPSGKILRKELR++LA+G 
Sbjct: 481 AFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmaCh08G005700 vs. TrEMBL
Match: A0A0A0K658_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430250 PE=4 SV=1)

HSP 1 Score: 959.1 bits (2478), Expect = 2.2e-276
Identity = 474/542 (87.45%), Postives = 512/542 (94.46%), Query Frame = 1

Query: 1   MAAEINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQ 60
           MA E N+T EFIFRSKLPDIHIPNHLPLH+Y+FQNL  + SRPCLINGATG  Y+Y DVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVA+GLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFTA 180
           AKAANAKLIVTMACFYDRVKDLAENGV++VCVD+AVEGCLH+SVLSGADES  P VDF++
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYY  +DVIL VLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDMS 300
           LNSILLCGLR GAAILIMQKF+IVSLL+L+EK++ SI+PIVPPI LAIAKS EFEKYD+S
Sbjct: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGAC 360
           SVRVLKSGGAPLGK+LEDAVREKFP A+LGQGYGMTEAGPVL+MSLAFAKEPF VK GAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKI+D ETG SLPANS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480
           IGF+DDDDELFIVDRLKELIKFK FQVAPAELEALLI HPKLSDAAVIGMPD +AGE+PV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540
           AFVV ANGG ITEE+VKQFI++QVVFYKRLKRVFFVNAIPKAPSGKILRKELR++LA+G 
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmaCh08G005700 vs. TrEMBL
Match: A0A0A0K6Q5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430230 PE=4 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 2.6e-269
Identity = 467/545 (85.69%), Postives = 504/545 (92.48%), Query Frame = 1

Query: 1   MAAEINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQ 60
           MA E NQT EFIFRSKLPDIHIPNHLPLH+Y+FQNL  + SRPCLINGATG VY+Y DVQ
Sbjct: 1   MAFESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVA+GLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFTA 180
           AKAANAKLIVTMACFYDRVKDL ENGVKIVCVD++ EGCLH+SVLSGADES +  VDF++
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYY+ +DVILCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDMS 300
           LNSILLCGLRAGAAI+IMQKF+IVSL +L+ KYK SIVPIVPPI LAIAKS+EFEKYD+S
Sbjct: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGAC 360
           SVRVLKSGGAPLGK+L +AV+ KFP AIL QGYGMTEAGPVLTMSLAFAKEPF VK  AC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKFEAC 360

Query: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKI+DPETGVSLP NSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGVSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480
           IGFIDD++ELFI+DRLKELIKFKTFQVAPAELEALLI HPKL DAAVIGMPD +AGE+PV
Sbjct: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVNANGG---EITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLA 540
           AFVV    G     TEE+VK+FI++QV+FYKRLKRVFFVNAIPKAPSGKILRKE+R+ LA
Sbjct: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKRLKRVFFVNAIPKAPSGKILRKEIRANLA 540

Query: 541 AGVYN 543
           +G YN
Sbjct: 541 SGAYN 545

BLAST of CmaCh08G005700 vs. TrEMBL
Match: S4W6Z9_CHOAR (Ca4CL3 OS=Chosenia arbutifolia PE=2 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 2.1e-234
Identity = 395/538 (73.42%), Postives = 468/538 (86.99%), Query Frame = 1

Query: 1   MAAEINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQ 60
           M   +N  +EFIFRSKLPDI+IP +LPLH Y+F+NL  + S+PCLINGA G VY+Y DV+
Sbjct: 1   MDTTMNPQEEFIFRSKLPDIYIPKNLPLHTYVFENLSKHSSKPCLINGANGDVYTYADVE 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVASGL+ +GI++GDV+M  LP+SPEFV  FLGAS+RGAI+TAANPF T  E+AK 
Sbjct: 61  LTARRVASGLNKIGIQQGDVIMLFLPSSPEFVLAFLGASHRGAIITAANPFSTPAELAKH 120

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFT 180
           AKA+ AKL++T AC+Y++VKD + ENGVKI+CVD A +GCLH+S L+ ADE+ +P VD  
Sbjct: 121 AKASKAKLLITQACYYEKVKDFSQENGVKIMCVDSAPDGCLHFSDLTQADENEMPQVDLN 180

Query: 181 ADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIY 240
            DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLY+   DVILCVLP FHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGENPNLYFHSEDVILCVLPMFHIY 240

Query: 241 SLNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDM 300
           +LNSI+LCGLR GA+ILIM KFEI SLL L+EKYK SI P+VPP++LAIAKS++ +K+D+
Sbjct: 241 ALNSIMLCGLRVGASILIMPKFEIGSLLGLIEKYKVSIAPVVPPVMLAIAKSADLDKHDL 300

Query: 301 SSVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGA 360
           SS+R+LKSGGAPLGK+LED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +KPGA
Sbjct: 301 SSLRMLKSGGAPLGKELEDTVRAKFPQARLGQGYGMTEAGPVLAMGLAFAKEPFDIKPGA 360

Query: 361 CGTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKI+DPETG SLP N  GEICIRGDQIMKGYLND E+T RTIDK+GWLHTG
Sbjct: 361 CGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYLNDPEATSRTIDKQGWLHTG 420

Query: 421 DIGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELP 480
           DIG+IDDDDELFIVDRLKELIK+K FQVAPAELEALL+AHP++SDAAV+GM DE AGE+P
Sbjct: 421 DIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLAHPEISDAAVVGMKDEDAGEVP 480

Query: 481 VAFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLA 538
           VAF V +   ++TE+++KQ+IS+QV+FYKR+KRVFF+ AIPKAPSGKILRK LR +LA
Sbjct: 481 VAFAVKSEKSQVTEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILRKNLREKLA 538

BLAST of CmaCh08G005700 vs. TrEMBL
Match: B2Z6P0_POPTR (4-coumarate:CoA ligase OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 4.0e-233
Identity = 395/537 (73.56%), Postives = 465/537 (86.59%), Query Frame = 1

Query: 1   MAAEINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQ 60
           M A +N  +EFIFRSKLPDI+IP +LPLH Y+ +NL  Y S+PCLINGA G V +Y DV+
Sbjct: 1   MDAIMNSQEEFIFRSKLPDIYIPKNLPLHSYVLENLSKYSSKPCLINGANGDVCTYADVE 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVASGL+ +GI++GDV+M  LP+SPEFV  FLGAS+RGAI+TAANPF T  E+AK 
Sbjct: 61  LTARRVASGLNKIGIQQGDVIMLFLPSSPEFVLAFLGASHRGAIVTAANPFSTPAELAKH 120

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFT 180
           AKA+ AKL++T AC+YD+VKD A E+ VK++CVD A +GCLH+S L+ ADE+ VP VDF+
Sbjct: 121 AKASRAKLLITQACYYDKVKDFARESDVKVMCVDSAPDGCLHFSELTQADENEVPQVDFS 180

Query: 181 ADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIY 240
            DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG NPNLY+   DVILCVLP FHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGDNPNLYFHSEDVILCVLPMFHIY 240

Query: 241 SLNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDM 300
           +LNSI+LCGLR GA+ILIM KF+I +LL L+EKYK SI P+VPP++LAIAKS +F+K+D+
Sbjct: 241 ALNSIMLCGLRVGASILIMPKFDIGTLLGLIEKYKVSIAPVVPPVMLAIAKSPDFDKHDL 300

Query: 301 SSVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGA 360
           SS+R++KSGGAPLGK+LED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +KPGA
Sbjct: 301 SSLRMIKSGGAPLGKELEDTVRAKFPQARLGQGYGMTEAGPVLAMCLAFAKEPFDIKPGA 360

Query: 361 CGTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKI+DPETG SLP N  GEICIRGDQIMKGYLND E+T RTIDKEGWLHTG
Sbjct: 361 CGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYLNDPEATSRTIDKEGWLHTG 420

Query: 421 DIGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELP 480
           DIG+IDDDDELFIVDRLKELIK+K FQVAPAELEALL+AHP++SDAAV+GM DE AGE+P
Sbjct: 421 DIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLAHPEISDAAVVGMKDEDAGEVP 480

Query: 481 VAFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQL 537
           VAFVV +   + TE+++KQ+IS+QV+FYKR+KRVFF+ AIPKAPSGKILRK LR  L
Sbjct: 481 VAFVVKSEKSQATEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILRKNLRETL 537

BLAST of CmaCh08G005700 vs. TAIR10
Match: AT1G51680.1 (AT1G51680.1 4-coumarate:CoA ligase 1)

HSP 1 Score: 747.7 bits (1929), Expect = 5.2e-216
Identity = 372/544 (68.38%), Postives = 445/544 (81.80%), Query Frame = 1

Query: 6   NQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQLTARR 65
           N   + IFRSKLPDI+IPNHL LH+YIFQN+  + ++PCLING TGHVY+Y DV + +R+
Sbjct: 18  NNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQ 77

Query: 66  VASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAAN 125
           +A+  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF+T  EIAKQAKA+N
Sbjct: 78  IAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASN 137

Query: 126 AKLIVTMACFYDRVKDLA-ENGVKIVCVDY-----AVEGCLHYSVL--SGADESHV-PFV 185
            KLI+T A + D++K L  ++GV IVC+D        EGCL ++ L  S  + S V   V
Sbjct: 138 TKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSV 197

Query: 186 DFTADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFF 245
           + + DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+  +DVILCVLP F
Sbjct: 198 EISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMF 257

Query: 246 HIYSLNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEK 305
           HIY+LNSI+LCGLR GAAILIM KFEI  LLEL+++ K ++ P+VPPI+LAIAKSSE EK
Sbjct: 258 HIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEK 317

Query: 306 YDMSSVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVK 365
           YD+SS+RV+KSG APLGK+LEDAV  KFP A LGQGYGMTEAGPVL MSL FAKEPFPVK
Sbjct: 318 YDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVK 377

Query: 366 PGACGTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWL 425
            GACGTVVRNAEMKI+DP+TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GWL
Sbjct: 378 SGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWL 437

Query: 426 HTGDIGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAG 485
           HTGDIG IDDDDELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AV+ M +E AG
Sbjct: 438 HTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAG 497

Query: 486 ELPVAFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQL 541
           E+PVAFVV +   E++E+DVKQF+S+QVVFYKR+ +VFF  +IPKAPSGKILRK+LR++L
Sbjct: 498 EVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 557

BLAST of CmaCh08G005700 vs. TAIR10
Match: AT3G21240.1 (AT3G21240.1 4-coumarate:CoA ligase 2)

HSP 1 Score: 745.0 bits (1922), Expect = 3.3e-215
Identity = 363/539 (67.35%), Postives = 448/539 (83.12%), Query Frame = 1

Query: 8   TKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQLTARRVA 67
           + + IFRS+LPDI+IPNHLPLH+YIF+N+  + ++PCLING TG VY+Y DV +T+R++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  SGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAK 127
           +GLHNLG+K+ DVVM LLPNSPE V TFL AS+ GAI T+ANPF+T  EI+KQAKA+ AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 LIVTMACFYDRVKDLAENGVKIVCVDYAV--EGCLHYSVLSGADESHVPFVD--FTADDV 187
           LIVT + + D++K+L  +GV IV  D     E CL +S L+ ++E  V  +    + +DV
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPEDV 197

Query: 188 VALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYSLNS 247
           VALP+SSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+ R+DVILCVLP FHIY+LNS
Sbjct: 198 VALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNS 257

Query: 248 ILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDMSSVR 307
           I+LC LR GA ILIM KFEI  LLE +++ K ++  +VPPI+LAIAKS E EKYD+SSVR
Sbjct: 258 IMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVR 317

Query: 308 VLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGACGTV 367
           ++KSG APLGK+LEDA+  KFP A LGQGYGMTEAGPVL MSL FAKEPFPVK GACGTV
Sbjct: 318 MVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTV 377

Query: 368 VRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGF 427
           VRNAEMKI+DP+TG SLP N  GEICIRG+QIMKGYLND  +T  TIDK+GWLHTGD+GF
Sbjct: 378 VRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGF 437

Query: 428 IDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPVAFV 487
           IDDDDELFIVDRLKELIK+K FQVAPAELE+LLI HP+++D AV+ M +E AGE+PVAFV
Sbjct: 438 IDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFV 497

Query: 488 VNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGVYN 543
           V +    I+E+++KQF+S+QVVFYKR+ +VFF ++IPKAPSGKILRK+LR++LA G+ N
Sbjct: 498 VRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLMN 556

BLAST of CmaCh08G005700 vs. TAIR10
Match: AT3G21230.1 (AT3G21230.1 4-coumarate:CoA ligase 5)

HSP 1 Score: 681.0 bits (1756), Expect = 5.9e-196
Identity = 329/546 (60.26%), Postives = 425/546 (77.84%), Query Frame = 1

Query: 4   EINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYG----SRPCLINGATGHVYSYDDV 63
           E   + +FIFRSKLPDI IPNHLPL +Y+FQ     G    S  C+I+GATG + +Y DV
Sbjct: 20  EEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADV 79

Query: 64  QLTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAK 123
           Q   RR+A+G+H LGI+ GDVVM LLPNSPEF  +FL  +Y GA+ T ANPFYT  EIAK
Sbjct: 80  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 139

Query: 124 QAKAANAKLIVTMACFYDRVKDLAENGVKIVCVD---------YAVEGCLHYSVLSGADE 183
           QAKA+ AK+I+T  C  D++ +L  +GV IVC+D          + +GC+ ++ L+ ADE
Sbjct: 140 QAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADE 199

Query: 184 SHVPFVDFTADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVIL 243
           + +     + +D VA+PYSSGTTGLPKGVM+THKGL+TS+AQ++DG+NPNL +  NDVIL
Sbjct: 200 TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVIL 259

Query: 244 CVLPFFHIYSLNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAK 303
           C LP FHIY+L++++L  +R GAA+LI+ +FE+  ++EL+++YK ++VP+ PP++LA  K
Sbjct: 260 CFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIK 319

Query: 304 SSEFEKYDMSSVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAK 363
           S E E+YD+SSVR++ SG A L K+LEDAVR KFP AI GQGYGMTE+G V   SLAFAK
Sbjct: 320 SPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAK 379

Query: 364 EPFPVKPGACGTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTI 423
            PF  K GACGTV+RNAEMK++D ETG+SLP N +GEIC+RG Q+MKGYLND E+T RTI
Sbjct: 380 NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTI 439

Query: 424 DKEGWLHTGDIGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGM 483
           DK+GWLHTGDIGF+DDDDE+FIVDRLKELIKFK +QVAPAELEALLI+HP + DAAV+ M
Sbjct: 440 DKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAM 499

Query: 484 PDEQAGELPVAFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRK 537
            DE A E+PVAFV  + G ++TE+DVK ++++QVV YKR+K VFF+  IPKA SGKILRK
Sbjct: 500 KDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRK 559

BLAST of CmaCh08G005700 vs. TAIR10
Match: AT1G65060.1 (AT1G65060.1 4-coumarate:CoA ligase 3)

HSP 1 Score: 674.1 bits (1738), Expect = 7.2e-194
Identity = 333/528 (63.07%), Postives = 419/528 (79.36%), Query Frame = 1

Query: 12  IFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQLTARRVASGLH 71
           IFRSKLPDI IPNHLPLH Y F+ L +   +PCLI G+TG  Y+Y +  L  RRVASGL+
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  NLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLIVT 131
            LGI+KGDV+M LL NS EFVF+F+GAS  GA+ T ANPFYT+ E+ KQ K++ AKLI+T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 MACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPF---VDFTADDVVALPY 191
            + + D++K+L EN   I   +   E CL +S L   DE++ PF   VD   DD  ALP+
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLITDDETN-PFQETVDIGGDDAAALPF 213

Query: 192 SSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYSLNSILLCG 251
           SSGTTGLPKGV+LTHK LITSVAQQ+DG NPNLY K NDVILCVLP FHIYSLNS+LL  
Sbjct: 214 SSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNS 273

Query: 252 LRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDMSSVRVLKSG 311
           LR+GA +L+M KFEI +LL+L+++++ +I  +VPP+++A+AK+     YD+SSVR + SG
Sbjct: 274 LRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLSG 333

Query: 312 GAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGACGTVVRNAE 371
            APLGK+L+D++R + P AILGQGYGMTEAGPVL+MSL FAKEP P K G+CGTVVRNAE
Sbjct: 334 AAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAE 393

Query: 372 MKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGFIDDDD 431
           +K++  ET +SL  N  GEICIRG QIMK YLND E+T  TID+EGWLHTGDIG++D+DD
Sbjct: 394 LKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDD 453

Query: 432 ELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPVAFVVNANG 491
           E+FIVDRLKE+IKFK FQV PAELE+LLI H  ++DAAV+   DE AGE+PVAFVV +NG
Sbjct: 454 EIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNG 513

Query: 492 GEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQL 537
            +ITEEDVK+++++QVVFYKRL +VFFV +IPK+PSGKILRK+L+++L
Sbjct: 514 NDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of CmaCh08G005700 vs. TAIR10
Match: AT4G05160.1 (AT4G05160.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 402.9 bits (1034), Expect = 3.1e-112
Identity = 220/548 (40.15%), Postives = 331/548 (60.40%), Query Frame = 1

Query: 9   KEFIFRSKLPDIHIPN--HLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQLTARRV 68
           ++ I+RS  P + +P   +  L  ++F+N  +Y S+  + +  TG   ++  ++    R+
Sbjct: 8   RDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARL 67

Query: 69  ASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANA 128
           A G H LGI+K DVV+   PNS +F   FL  +  G + T ANP YT  E++KQ K +N 
Sbjct: 68  AHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNP 127

Query: 129 KLIVTMACFYDRVK------------DLAE----NGVKIVCVDYAVEGCLHYSVLSGADE 188
           K+I+++   +D++K            D  E    +  KI+  D  +E             
Sbjct: 128 KIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVME--------LSEPV 187

Query: 189 SHVPFVDFTADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVIL 248
           S  PFV+    D  AL YSSGTTG  KGV LTH   I   A  +   + +L  + + V L
Sbjct: 188 SEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIA--ASLMVTMDQDLMGEYHGVFL 247

Query: 249 CVLPFFHIYSLNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAK 308
           C LP FH++ L  I    L+ G A++ M +FE+  +L+ +EK++ + + +VPP+ LA++K
Sbjct: 248 CFLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSK 307

Query: 309 SSEFEKYDMSSVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAK 368
            S  +K+D+SS++ + SG APLGK L +      P  +L QGYGMTE     T  +   +
Sbjct: 308 QSIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTE-----TCGIVSVE 367

Query: 369 EPFPVK--PGACGTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKR 428
           +P   K   G+ G +    E +I+  ETG S P N  GEI +RG  +MKGYLN+ ++TK 
Sbjct: 368 DPRLGKRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKE 427

Query: 429 TIDKEGWLHTGDIGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVI 488
           TIDK+ W+HTGD+G+ ++D  L++VDR+KELIK+K FQVAPAELE LL++HP + DA VI
Sbjct: 428 TIDKKSWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVI 487

Query: 489 GMPDEQAGELPVAFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKIL 537
             PDE+AGE+P+AFVV +    ITE+D+++FI++QV  YKRL+RV F++ +PK+ +GKIL
Sbjct: 488 PFPDEEAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKIL 540

BLAST of CmaCh08G005700 vs. NCBI nr
Match: gi|659122577|ref|XP_008461216.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo])

HSP 1 Score: 968.4 bits (2502), Expect = 5.3e-279
Identity = 479/542 (88.38%), Postives = 514/542 (94.83%), Query Frame = 1

Query: 1   MAAEINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQ 60
           MA E NQT+EFIFRSKLPDIHIPNHLPLH+Y+FQN+P + +RPCLINGATG VY+Y DVQ
Sbjct: 1   MAFESNQTQEFIFRSKLPDIHIPNHLPLHQYVFQNMPKFATRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVA+GLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFTA 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVD+AVEGCLH+SVLSGADES  P VDF++
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESLAPVVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYY  +DVIL VLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDMS 300
           LNSILLCGLR GA ILIMQKF+IVSLL+L+EK+K SI+PIVPPI LAIAKS EFEKYD+S
Sbjct: 241 LNSILLCGLRVGATILIMQKFDIVSLLQLIEKHKISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGAC 360
           SVRVLKSGGAPLGK+LEDAVREKFPTA+LGQGYGMTEAGPVL+MSLAFAKEPF VK GAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKI+D ETG SLPANS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480
           IGF+DDDDELFIVDRLKELIKFK FQVAPAELEALLI HPKLSDAAVIGMPD +AGE+PV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540
           AFVV ANGG ITEE+VKQFI++QVVFYKRLKRVFFVNAIPKAPSGKILRKELR++LA G 
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLALGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmaCh08G005700 vs. NCBI nr
Match: gi|778728825|ref|XP_004135894.2| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus])

HSP 1 Score: 967.2 bits (2499), Expect = 1.2e-278
Identity = 478/542 (88.19%), Postives = 516/542 (95.20%), Query Frame = 1

Query: 1   MAAEINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQ 60
           MA E N+T EFIFRSKLPDIHIPNHLPLH+Y+FQNL  + SRPCLINGATG VY+Y DVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVA+GLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFTA 180
           AKAANAKLIVTMACFYDRVKDLAENGV+IVCVD+AVEGCLH+SVLSGADES  P VDF++
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYY RNDVILCVLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDMS 300
           LNSILLCGLR GAAI+IMQKF+IV+LL+L+EK++ SI+PIVPPI LAIAKS EFEKYD+S
Sbjct: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGAC 360
           SVRVLKSGGAPLGK+LEDAVREKFPTAILGQGYGMTEAGPVL+MSLAFAKEPF VK GAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKI+D ETG SLPANS+GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480
           IGF+DDD+ELFIVDRLKELIKFK FQVAPAELEALLI HPKLSDAAVIGMPD +AGE+PV
Sbjct: 421 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540
           AFV+ ANGG I+EE+VKQFI++QVVFYKRLKRVFFVNAIPKAPSGKILRKELR++LA+G 
Sbjct: 481 AFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmaCh08G005700 vs. NCBI nr
Match: gi|778728828|ref|XP_004135893.2| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus])

HSP 1 Score: 959.1 bits (2478), Expect = 3.2e-276
Identity = 474/542 (87.45%), Postives = 512/542 (94.46%), Query Frame = 1

Query: 1   MAAEINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQ 60
           MA E N+T EFIFRSKLPDIHIPNHLPLH+Y+FQNL  + SRPCLINGATG  Y+Y DVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVA+GLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFTA 180
           AKAANAKLIVTMACFYDRVKDLAENGV++VCVD+AVEGCLH+SVLSGADES  P VDF++
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYY  +DVIL VLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDMS 300
           LNSILLCGLR GAAILIMQKF+IVSLL+L+EK++ SI+PIVPPI LAIAKS EFEKYD+S
Sbjct: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGAC 360
           SVRVLKSGGAPLGK+LEDAVREKFP A+LGQGYGMTEAGPVL+MSLAFAKEPF VK GAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKI+D ETG SLPANS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480
           IGF+DDDDELFIVDRLKELIKFK FQVAPAELEALLI HPKLSDAAVIGMPD +AGE+PV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540
           AFVV ANGG ITEE+VKQFI++QVVFYKRLKRVFFVNAIPKAPSGKILRKELR++LA+G 
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmaCh08G005700 vs. NCBI nr
Match: gi|659122579|ref|XP_008461217.1| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 954.1 bits (2465), Expect = 1.0e-274
Identity = 474/542 (87.45%), Postives = 512/542 (94.46%), Query Frame = 1

Query: 1   MAAEINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQ 60
           MA E N+T EFIFRSKLPDIHIPNHLPLH+Y+FQNL  + SRPCLINGATG VY+Y DVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVA+GLHNLGIKKGDVVM+LLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMHLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFTA 180
           AKAA+AKLIVTMACFYDRVKDL ENGVKIVCVD+AVEGCLH+SVLSGADES  P VDF++
Sbjct: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYY  NDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDMS 300
           LNSILLCGLR G+AILI+QKF+IVSLL+L+EK++ SI+PIVPPI LAIAKS EFEKYD+S
Sbjct: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGAC 360
           SVRVLKSGGAPLGK+LEDAVREKFPTAILGQGYGMTEAGPVL+MSLAFAKEPF VK GAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKI+D ETG SLPANS+GEICIRG+QIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480
           IGF+D+D+ELFIVDRLKELIKFK FQVAPAELEALLI HPKLSDAAVIGMPD +AGE+PV
Sbjct: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540
           AFVV A GG ITEE+VKQFI++QVVFYKRLKRV FVNAIPKAPSGKILRKELR++LA+G 
Sbjct: 481 AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmaCh08G005700 vs. NCBI nr
Match: gi|659122581|ref|XP_008461218.1| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 941.4 bits (2432), Expect = 6.9e-271
Identity = 468/542 (86.35%), Postives = 503/542 (92.80%), Query Frame = 1

Query: 1   MAAEINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQ 60
           MA E NQT EFIFRSKLPDIHIPNHLPLH+Y+FQNL  + SRPCLINGATG VYSY DV 
Sbjct: 1   MAFESNQTHEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYYDVN 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVA+GLH+LGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFTA 180
            KAANAKLIVTMACFYDRVKDLAENGVKIVCVD++VEGCLH+SVLSGADES +P VDF++
Sbjct: 121 VKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFSVEGCLHFSVLSGADESLMPRVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYY+ +DVILCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDMS 300
           LNSILLCGLRAGAAI IMQKF+IVSL +L+ KYK SIVPIVPPI LAIAKS EFEKYD+S
Sbjct: 241 LNSILLCGLRAGAAITIMQKFDIVSLFQLIGKYKVSIVPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGAC 360
           SVRVLKSGGAPLGK+L +AV+ KFP AIL QGYGMTEAGPVLTMSLAFAKEPF VK  AC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAEAC 360

Query: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKI+DPETG SLP NS GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPENSPGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480
           IGF+DD++ELFI+DRLKELIKFKTFQVAPAELEALLIAHPKL DAAVIGMPD +AGE+PV
Sbjct: 421 IGFVDDNNELFIIDRLKELIKFKTFQVAPAELEALLIAHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540
           AFVV    G  TEE+VKQF+++QVVFYKRLKRVFFVNAIPKAPSGKILRKELR++LA+G 
Sbjct: 481 AFVVEEKSGATTEEEVKQFVTKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
4CL2_TOBAC2.5e-22872.474-coumarate--CoA ligase 2 OS=Nicotiana tabacum GN=4CL2 PE=1 SV=1[more]
4CL1_TOBAC4.7e-22771.114-coumarate--CoA ligase 1 OS=Nicotiana tabacum GN=4CL1 PE=2 SV=1[more]
4CL_VANPL3.0e-22671.304-coumarate--CoA ligase OS=Vanilla planifolia GN=4CL PE=3 SV=1[more]
4CL2_SOLTU6.3e-22469.984-coumarate--CoA ligase 2 OS=Solanum tuberosum GN=4CL2 PE=3 SV=1[more]
4CL1_SOLTU8.3e-22469.804-coumarate--CoA ligase 1 OS=Solanum tuberosum GN=4CL1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K8H9_CUCSA8.2e-27988.19Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430240 PE=4 SV=1[more]
A0A0A0K658_CUCSA2.2e-27687.45Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430250 PE=4 SV=1[more]
A0A0A0K6Q5_CUCSA2.6e-26985.69Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430230 PE=4 SV=1[more]
S4W6Z9_CHOAR2.1e-23473.42Ca4CL3 OS=Chosenia arbutifolia PE=2 SV=1[more]
B2Z6P0_POPTR4.0e-23373.564-coumarate:CoA ligase OS=Populus trichocarpa PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G51680.15.2e-21668.38 4-coumarate:CoA ligase 1[more]
AT3G21240.13.3e-21567.35 4-coumarate:CoA ligase 2[more]
AT3G21230.15.9e-19660.26 4-coumarate:CoA ligase 5[more]
AT1G65060.17.2e-19463.07 4-coumarate:CoA ligase 3[more]
AT4G05160.13.1e-11240.15 AMP-dependent synthetase and ligase family protein[more]
Match NameE-valueIdentityDescription
gi|659122577|ref|XP_008461216.1|5.3e-27988.38PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo][more]
gi|778728825|ref|XP_004135894.2|1.2e-27888.19PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus][more]
gi|778728828|ref|XP_004135893.2|3.2e-27687.45PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus][more]
gi|659122579|ref|XP_008461217.1|1.0e-27487.45PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo][more]
gi|659122581|ref|XP_008461218.1|6.9e-27186.35PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000873AMP-dep_Synth/Lig
IPR020845AMP-binding_CS
IPR025110AMP-bd_C
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009805 coumarin biosynthetic process
biological_process GO:0009809 lignin biosynthetic process
biological_process GO:0009811 stilbene biosynthetic process
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016207 4-coumarate-CoA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0016874 ligase activity
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh08G005700.1CmaCh08G005700.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 39..442
score: 2.3E
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 186..197
scor
IPR025110AMP-binding enzyme C-terminal domainPFAMPF13193AMP-binding_Ccoord: 451..526
score: 4.4
NoneNo IPR availableGENE3DG3DSA:2.30.38.10coord: 357..433
score: 1.1
NoneNo IPR availableGENE3DG3DSA:3.30.300.30coord: 439..536
score: 2.3
NoneNo IPR availableGENE3DG3DSA:3.40.50.980coord: 28..191
score: 7.1E-33coord: 192..347
score: 1.2
NoneNo IPR availablePANTHERPTHR24096FAMILY NOT NAMEDcoord: 3..535
score:
NoneNo IPR availablePANTHERPTHR24096:SF1494-COUMARATE--COA LIGASE 1-RELATEDcoord: 3..535
score:
NoneNo IPR availableunknownSSF56801Acetyl-CoA synthetase-likecoord: 10..538
score: 1.83E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh08G005700Cucsa.106170Cucumber (Gy14) v1cgycmaB0241
CmaCh08G005700Cucsa.049830Cucumber (Gy14) v1cgycmaB0052
CmaCh08G005700Cla015298Watermelon (97103) v1cmawmB857
CmaCh08G005700Cla017226Watermelon (97103) v1cmawmB862
CmaCh08G005700Csa3G710200Cucumber (Chinese Long) v2cmacuB886
CmaCh08G005700Csa7G430230Cucumber (Chinese Long) v2cmacuB914
CmaCh08G005700MELO3C023494Melon (DHL92) v3.5.1cmameB824
CmaCh08G005700ClCG10G012790Watermelon (Charleston Gray)cmawcgB792
CmaCh08G005700ClCG09G003580Watermelon (Charleston Gray)cmawcgB787
CmaCh08G005700CSPI03G30180Wild cucumber (PI 183967)cmacpiB904
CmaCh08G005700CSPI07G19800Wild cucumber (PI 183967)cmacpiB934
CmaCh08G005700CmoCh08G005640Cucurbita moschata (Rifu)cmacmoB913
CmaCh08G005700CmoCh01G002070Cucurbita moschata (Rifu)cmacmoB905
CmaCh08G005700CmoCh17G008870Cucurbita moschata (Rifu)cmacmoB896
CmaCh08G005700CmoCh14G010570Cucurbita moschata (Rifu)cmacmoB879
CmaCh08G005700Lsi02G026350Bottle gourd (USVL1VR-Ls)cmalsiB822
CmaCh08G005700Lsi03G012450Bottle gourd (USVL1VR-Ls)cmalsiB831
CmaCh08G005700MELO3C023494.2Melon (DHL92) v3.6.1cmamedB922
CmaCh08G005700CsaV3_7G031600Cucumber (Chinese Long) v3cmacucB1084
CmaCh08G005700Cla97C10G195660Watermelon (97103) v2cmawmbB910
CmaCh08G005700Cla97C09G165820Watermelon (97103) v2cmawmbB941
CmaCh08G005700Bhi09G000458Wax gourdcmawgoB1128
CmaCh08G005700CsGy3G027980Cucumber (Gy14) v2cgybcmaB456
CmaCh08G005700CsGy7G018090Cucumber (Gy14) v2cgybcmaB973
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh08G005700CmaCh14G010390Cucurbita maxima (Rimu)cmacmaB293
CmaCh08G005700CmaCh17G009160Cucurbita maxima (Rimu)cmacmaB382
CmaCh08G005700CmaCh01G002000Cucurbita maxima (Rimu)cmacmaB440
The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh08G005700Silver-seed gourdcarcmaB0499
CmaCh08G005700Silver-seed gourdcarcmaB0628
CmaCh08G005700Cucurbita pepo (Zucchini)cmacpeB902
CmaCh08G005700Cucurbita pepo (Zucchini)cmacpeB917