CSPI07G19800 (gene) Wild cucumber (PI 183967)

NameCSPI07G19800
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
Description4-coumarate--CoA ligase
LocationChr7 : 17325318 .. 17327238 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCATCTCTACTTTTTTTCTCTTAATTACTCTCTGTTTCTACTCTGTCTTTCTTCAGACCTCAACAATGGCCTTTGAATCAAACCAAACTAATGAATTCATTTTCCGTTCAAAGCTTCCTAATATTCACATTCCCAATCATCTCCCACTTCACGATTATGTTTTTCAAAATCTCTCAAAATTTGCCTCACGCCCATGTTTGATCAACGGCGCCACCGGGGATGTTTACACCTACCATGACGTTCAGCTAACAGCCCGTCGAGTCGCCGCTGGCCTACACAACCTCGGTATTAAGAAGGGTGATGTTGTCATGAATTTACTTCCCAATTCCCCGGAGTTCGTCTTCACTTTCCTTGGCGCTTCTTACCGGGGCGCCATCATGACGGCAGCCAACCCTTTTTACACTGCGGTGGAAATAGCCAAACAGGCCAAAGCCGCCAATGCGAAATTAATCGTGACTATGGCTTGCTTCTACGATCGGGTTAAGGATTTGGGTGAAAATGGTGTCAAAATCGTATGTGTTGATTTTTCTGCTGAGGGTTGTTTGCATTTCTCTGTTTTGAGTGGGGCTGACGAATCTCTAATGTCGCGGGTGGATTTTTCTTCCGATGATGTGGTGGCGCTACCGTACTCCTCTGGGACTACCGGTTTGCCAAAGGGAGTTATGTTGACTCATAAAGGGTTAATCACAAGTGTGGCTCAACAAGTCGACGGCCAAAATCCGAACCTATATTATCGTGGCGATGATGTAATCCTCTGTGTACTGCCGCTTTTCCATATCTATTCACTCAATTCGATTTTGCTGTGTGGGCTACGCGCCGGTGCTGCCATTATGATTATGCAAAAGTTCGACATCGTTTCACTTTTTCAATTGATTGGGAAATACAAAATCTCGATCGTGCCCATCGTGCCGCCAATCTTCTTGGCCATTGCTAAGTCAGCTGAGTTTGAAAAATACGACGTGTCGTCCGTAAGGGTTCTAAAATCCGGCGGAGCACCACTGGGGAAGGAGTTGGTAGAGGCCGTGAAGGCAAAATTTCCAGCGGCGATTCTCGCACAAGGGTACGGAATGACTGAGGCTGGTCCGGTTCTAACTATGAGTTTGGCTTTTGCAAAAGAACCGTTTCAAGTGAAATCCGAAGCATGCGGAACTGTGGTCAGAAATGCAGAGATGAAGATAGTCGACCCGGAAACCGGCTCCTCCTTACCTGAAAATTCCGCAGGTGAGATTTGTATTAGAGGTGATCAAATCATGAAGGGATATTTGAATGATTTGGAGTCGACCAAGAGGACTATTGATAAAGAAGGATGGCTTCACACCGGCGACATCGGCTTCATCGATGACAACAATGAGCTCTTCATTATAGACCGGCTTAAGGAGCTTATCAAGTTCAAAACATTTCAAGTGGCACCCGCCGAACTGGAAGCACTTCTTATCACTCACCCAAAACTACGTGACGCTGCTGTTATTGGGTGAGTTCAAATCCTATTTGGTTCAAAATGGCATTTGTGAGATTAAATTTTTAATGGGATTTTTCTTTGCATATTGTTGTTTTTAGTATGCCAGACGTGGAGGCAGGAGAAGTACCGGTGGCGTTTGTGGTGGAGGAGAAGAGTGGTGCAAGTGCAACAACAACAGAAGAAGAAGTAAAAGAATTCATAGCAAAGCAAGTGATTTTCTACAAAAAGCTAAAGCGTGTGTTTTTTGTCAACGCTATTCCAAAGGCACCATCTGGCAAAATCCTCAGGAAAGAAATAAGGGCAAATTTGGCATCCGGCGCTTACAATTAATTAAACCCTAATCTTAGTTATTCATTTATTTATTAGCCTTCTGTTTGGTTTGTTAATTAATGCAAATTATGTATTAGGATTGTGAAACACAATTCTTACCTGTAATTGTATATTTTATTTTACAATAATAA

mRNA sequence

ATGGCCTTTGAATCAAACCAAACTAATGAATTCATTTTCCGTTCAAAGCTTCCTAATATTCACATTCCCAATCATCTCCCACTTCACGATTATGTTTTTCAAAATCTCTCAAAATTTGCCTCACGCCCATGTTTGATCAACGGCGCCACCGGGGATGTTTACACCTACCATGACGTTCAGCTAACAGCCCGTCGAGTCGCCGCTGGCCTACACAACCTCGGTATTAAGAAGGGTGATGTTGTCATGAATTTACTTCCCAATTCCCCGGAGTTCGTCTTCACTTTCCTTGGCGCTTCTTACCGGGGCGCCATCATGACGGCAGCCAACCCTTTTTACACTGCGGTGGAAATAGCCAAACAGGCCAAAGCCGCCAATGCGAAATTAATCGTGACTATGGCTTGCTTCTACGATCGGGTTAAGGATTTGGGTGAAAATGGTGTCAAAATCGTATGTGTTGATTTTTCTGCTGAGGGTTGTTTGCATTTCTCTGTTTTGAGTGGGGCTGACGAATCTCTAATGTCGCGGGTGGATTTTTCTTCCGATGATGTGGTGGCGCTACCGTACTCCTCTGGGACTACCGGTTTGCCAAAGGGAGTTATGTTGACTCATAAAGGGTTAATCACAAGTGTGGCTCAACAAGTCGACGGCCAAAATCCGAACCTATATTATCGTGGCGATGATGTAATCCTCTGTGTACTGCCGCTTTTCCATATCTATTCACTCAATTCGATTTTGCTGTGTGGGCTACGCGCCGGTGCTGCCATTATGATTATGCAAAAGTTCGACATCGTTTCACTTTTTCAATTGATTGGGAAATACAAAATCTCGATCGTGCCCATCGTGCCGCCAATCTTCTTGGCCATTGCTAAGTCAGCTGAGTTTGAAAAATACGACGTGTCGTCCGTAAGGGTTCTAAAATCCGGCGGAGCACCACTGGGGAAGGAGTTGGTAGAGGCCGTGAAGGCAAAATTTCCAGCGGCGATTCTCGCACAAGGGTACGGAATGACTGAGGCTGGTCCGGTTCTAACTATGAGTTTGGCTTTTGCAAAAGAACCGTTTCAAGTGAAATCCGAAGCATGCGGAACTGTGGTCAGAAATGCAGAGATGAAGATAGTCGACCCGGAAACCGGCTCCTCCTTACCTGAAAATTCCGCAGGTGAGATTTGTATTAGAGGTGATCAAATCATGAAGGGATATTTGAATGATTTGGAGTCGACCAAGAGGACTATTGATAAAGAAGGATGGCTTCACACCGGCGACATCGGCTTCATCGATGACAACAATGAGCTCTTCATTATAGACCGGCTTAAGGAGCTTATCAAGTTCAAAACATTTCAAGTGGCACCCGCCGAACTGGAAGCACTTCTTATCACTCACCCAAAACTACGTGACGCTGCTGTTATTGGTATGCCAGACGTGGAGGCAGGAGAAGTACCGGTGGCGTTTGTGGTGGAGGAGAAGAGTGGTGCAAGTGCAACAACAACAGAAGAAGAAGTAAAAGAATTCATAGCAAAGCAAGTGATTTTCTACAAAAAGCTAAAGCGTGTGTTTTTTGTCAACGCTATTCCAAAGGCACCATCTGGCAAAATCCTCAGGAAAGAAATAAGGGCAAATTTGGCATCCGGCGCTTACAATTAA

Coding sequence (CDS)

ATGGCCTTTGAATCAAACCAAACTAATGAATTCATTTTCCGTTCAAAGCTTCCTAATATTCACATTCCCAATCATCTCCCACTTCACGATTATGTTTTTCAAAATCTCTCAAAATTTGCCTCACGCCCATGTTTGATCAACGGCGCCACCGGGGATGTTTACACCTACCATGACGTTCAGCTAACAGCCCGTCGAGTCGCCGCTGGCCTACACAACCTCGGTATTAAGAAGGGTGATGTTGTCATGAATTTACTTCCCAATTCCCCGGAGTTCGTCTTCACTTTCCTTGGCGCTTCTTACCGGGGCGCCATCATGACGGCAGCCAACCCTTTTTACACTGCGGTGGAAATAGCCAAACAGGCCAAAGCCGCCAATGCGAAATTAATCGTGACTATGGCTTGCTTCTACGATCGGGTTAAGGATTTGGGTGAAAATGGTGTCAAAATCGTATGTGTTGATTTTTCTGCTGAGGGTTGTTTGCATTTCTCTGTTTTGAGTGGGGCTGACGAATCTCTAATGTCGCGGGTGGATTTTTCTTCCGATGATGTGGTGGCGCTACCGTACTCCTCTGGGACTACCGGTTTGCCAAAGGGAGTTATGTTGACTCATAAAGGGTTAATCACAAGTGTGGCTCAACAAGTCGACGGCCAAAATCCGAACCTATATTATCGTGGCGATGATGTAATCCTCTGTGTACTGCCGCTTTTCCATATCTATTCACTCAATTCGATTTTGCTGTGTGGGCTACGCGCCGGTGCTGCCATTATGATTATGCAAAAGTTCGACATCGTTTCACTTTTTCAATTGATTGGGAAATACAAAATCTCGATCGTGCCCATCGTGCCGCCAATCTTCTTGGCCATTGCTAAGTCAGCTGAGTTTGAAAAATACGACGTGTCGTCCGTAAGGGTTCTAAAATCCGGCGGAGCACCACTGGGGAAGGAGTTGGTAGAGGCCGTGAAGGCAAAATTTCCAGCGGCGATTCTCGCACAAGGGTACGGAATGACTGAGGCTGGTCCGGTTCTAACTATGAGTTTGGCTTTTGCAAAAGAACCGTTTCAAGTGAAATCCGAAGCATGCGGAACTGTGGTCAGAAATGCAGAGATGAAGATAGTCGACCCGGAAACCGGCTCCTCCTTACCTGAAAATTCCGCAGGTGAGATTTGTATTAGAGGTGATCAAATCATGAAGGGATATTTGAATGATTTGGAGTCGACCAAGAGGACTATTGATAAAGAAGGATGGCTTCACACCGGCGACATCGGCTTCATCGATGACAACAATGAGCTCTTCATTATAGACCGGCTTAAGGAGCTTATCAAGTTCAAAACATTTCAAGTGGCACCCGCCGAACTGGAAGCACTTCTTATCACTCACCCAAAACTACGTGACGCTGCTGTTATTGGTATGCCAGACGTGGAGGCAGGAGAAGTACCGGTGGCGTTTGTGGTGGAGGAGAAGAGTGGTGCAAGTGCAACAACAACAGAAGAAGAAGTAAAAGAATTCATAGCAAAGCAAGTGATTTTCTACAAAAAGCTAAAGCGTGTGTTTTTTGTCAACGCTATTCCAAAGGCACCATCTGGCAAAATCCTCAGGAAAGAAATAAGGGCAAATTTGGCATCCGGCGCTTACAATTAA
BLAST of CSPI07G19800 vs. Swiss-Prot
Match: 4CL2_TOBAC (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum GN=4CL2 PE=1 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 2.3e-221
Identity = 381/537 (70.95%), Postives = 451/537 (83.99%), Query Frame = 1

Query: 10  EFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRVAAG 69
           + IFRSKLP+I+IPNHLPLH Y F+N+S+F+SRPCLINGA   +YTY DV+L +R+VAAG
Sbjct: 9   DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 70  LHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLI 129
           LH  GI+  D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA++AK+I
Sbjct: 69  LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 130 VTMACFYDRVKDLG-ENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSSDDVVALPY 189
           VT AC  ++VKD   EN VKI+C+D + EGCLHFSVL+ A+E  +  V+   DDVVALPY
Sbjct: 129 VTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVVALPY 188

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQVDG+NPNLY   +DV+LCVLPLFHIYSLNS+LLCG
Sbjct: 189 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLLCG 248

Query: 250 LRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVSSVRVLKSG 309
           LR GAAI+IMQKFDIVS  +LI +YK++I P VPPI LAIAKS   + YD+SSVR + SG
Sbjct: 249 LRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRTVMSG 308

Query: 310 GAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEACGTVVRNAE 369
            APLGKEL + V+AKFP A L QGYGMTEAGPVL M LAFAKEPF++KS ACGTVVRNAE
Sbjct: 309 AAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAE 368

Query: 370 MKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGFIDDNN 429
           MKIVDP+TG+SLP N +GEICIRGDQIMKGYLND E+T RTIDKEGWL+TGDIG+IDD++
Sbjct: 369 MKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDDDD 428

Query: 430 ELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPVAFVVEEKS 489
           ELFI+DRLKELIK+K FQVAPAELEALL+ HP + DAAV+ M D +AGEVPVAFVV    
Sbjct: 429 ELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSN- 488

Query: 490 GASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLASGAYN 546
              +T TE+EVK+FI+KQVIFYK++KRVFFV+AIPK+PSGKILRK++RA LA+G  N
Sbjct: 489 --GSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542

BLAST of CSPI07G19800 vs. Swiss-Prot
Match: 4CL_VANPL (4-coumarate--CoA ligase OS=Vanilla planifolia GN=4CL PE=3 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 7.3e-220
Identity = 384/543 (70.72%), Postives = 450/543 (82.87%), Query Frame = 1

Query: 7   QTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRV 66
           Q  + IFRSKLP+I+IP +LPLH Y F+N+SKF+SRPCLINGAT +++TY DV+L +RRV
Sbjct: 10  QKKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVELISRRV 69

Query: 67  AAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANA 126
            +GL  LGIK+GD +M LLPNSPEFVF FLGAS+ G+I T ANPF+T+ E+ KQAKA+NA
Sbjct: 70  GSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQAKASNA 129

Query: 127 KLIVTMACFYDRVKDLG-ENGVKIVCVDFS-----AEGCLHFSVLSGADESLMSRVDFSS 186
           KLI+T  C+ D+VKD   ENGVKI+ +D +     A   LHFS L+GADE+ M +V+ S 
Sbjct: 130 KLIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADENEMPKVEISP 189

Query: 187 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 246
           D VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQVDG+NPNLY   DDV+LCVLPLFHIYS
Sbjct: 190 DGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVLPLFHIYS 249

Query: 247 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 306
           LNS+LLCGLRAG+ I+IMQKF+IV   +LI KYK++I P VPPI LAIAKS   + YD+S
Sbjct: 250 LNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTVVDNYDLS 309

Query: 307 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 366
           SVR + SG APLGKEL +AV+AKFP A L QGYGMTEAGPVL M LAFAKEPF +KS AC
Sbjct: 310 SVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGAC 369

Query: 367 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 426
           GTVVRNAEMKIVDPETGSSLP N  GEICIRGDQIMKGYLND E+T RTIDKEGWLHTGD
Sbjct: 370 GTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKEGWLHTGD 429

Query: 427 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 486
           IG+IDD++ELFI+DRLKELIK+K FQVAPAELEALL+THP + DAAV+ M D  AGEVPV
Sbjct: 430 IGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEAAGEVPV 489

Query: 487 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 544
           AFVV+         TE+E+K+FI+KQVIFYK++ RVFFV AIPKAPSGKILRK++RA LA
Sbjct: 490 AFVVKSN---GHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRARLA 549

BLAST of CSPI07G19800 vs. Swiss-Prot
Match: 4CL1_TOBAC (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum GN=4CL1 PE=2 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 1.1e-218
Identity = 375/543 (69.06%), Postives = 444/543 (81.77%), Query Frame = 1

Query: 4   ESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTA 63
           E+ Q+ + IFRSKLP+I+IP HLPLH Y F+N+S+F+SRPCLINGA   +YTY +V+LT 
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 64  RRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKA 123
           R+VA GL+ LGI++ D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 124 ANAKLIVTMACFYDRVKDLG-ENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSSDD 183
           ++AK+I+T +CF  +VKD   EN VK++C+D + EGCLHFS L+ +DE  +  V    DD
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDD 187

Query: 184 VVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYSLN 243
           VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQVDG+N NLY   +DV++CVLPLFHIYSLN
Sbjct: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 247

Query: 244 SILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVSSV 303
           SILLCGLR GAAI+IMQKFDI    +LI KYK+SI P VPPI LAIAKS   + YD+SSV
Sbjct: 248 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 307

Query: 304 RVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEACGT 363
           R + SG APLGKEL +AV+ KFP A L QGYGMTEAGPVL M LAFAKEPF +KS ACGT
Sbjct: 308 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 367

Query: 364 VVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIG 423
           VVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND E+T RTIDKEGWLHTGDIG
Sbjct: 368 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 427

Query: 424 FIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPVAF 483
           FID+++ELFI+DRLKELIK+K FQVAPAE+EALL+ HP + DAAV+ M D +AGEVPVAF
Sbjct: 428 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 487

Query: 484 VVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLASG 543
           VV     A    TE+EVK+FI+KQVIFYK++KRVFFV  +PK+PSGKILRK++RA LA+G
Sbjct: 488 VVRSNGSA---ITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAG 547

Query: 544 AYN 546
             N
Sbjct: 548 VPN 547

BLAST of CSPI07G19800 vs. Swiss-Prot
Match: 4CL1_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum GN=4CL1 PE=2 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 7.5e-217
Identity = 370/534 (69.29%), Postives = 444/534 (83.15%), Query Frame = 1

Query: 10  EFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRVAAG 69
           + IFRSKLP+I+IP HLPLH Y F+N+SK   + CLINGATG+ +TY  V+L +R+VA+G
Sbjct: 10  DLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVASG 69

Query: 70  LHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLI 129
           L+ LGI++GD +M LLPNSPE+ F FLGASYRGAI T ANPF+T+ E+ KQ KA+ AKLI
Sbjct: 70  LNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKLI 129

Query: 130 VTMACFYDRVKDLG-ENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSSDDVVALPY 189
           +T AC+ D+VKD   E  ++I+C+D + + CLHFS L  ADES M  V  +SDDVVALPY
Sbjct: 130 ITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVVINSDDVVALPY 189

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQVDG NPNLY   +DV++C+LPLFHIYSLN++L CG
Sbjct: 190 SSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAVLCCG 249

Query: 250 LRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVSSVRVLKSG 309
           LRAG  I+IMQKFDIV   +LI KYK++I P VPPI LAIAKS   +KYD+SSVR + SG
Sbjct: 250 LRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVMSG 309

Query: 310 GAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEACGTVVRNAE 369
            APLGKEL +AV+AKFP A L QGYGMTEAGPVL M LAFAKEP+++KS ACGTVVRNAE
Sbjct: 310 AAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAE 369

Query: 370 MKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGFIDDNN 429
           MKIVDPET +SLP N  GEICIRGDQIMKGYLND EST+ TID+EGWLHTGDIGFIDD++
Sbjct: 370 MKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDDDD 429

Query: 430 ELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPVAFVVEEKS 489
           ELFI+DRLKE+IK+K FQVAPAELEALL+THP + DAAV+ M D +AGEVPVAFVV    
Sbjct: 430 ELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRTN- 489

Query: 490 GASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLASG 543
               TTTEEE+K+F++KQV+FYK++ RVFFV+AIPK+PSGKILRK++RA +ASG
Sbjct: 490 --GFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARIASG 540

BLAST of CSPI07G19800 vs. Swiss-Prot
Match: 4CL2_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum GN=4CL2 PE=2 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 2.2e-216
Identity = 370/534 (69.29%), Postives = 443/534 (82.96%), Query Frame = 1

Query: 10  EFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRVAAG 69
           + IFRSKLP+I+IP HLPLH Y F+N+SK   + CLINGATG+ +TY  V+L +R+VA+G
Sbjct: 10  DLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVASG 69

Query: 70  LHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLI 129
           L+ LGI++GD +M LLPNSPE+ F FLGASYRGAI T ANPF+T+ E+ KQ KA+ AKLI
Sbjct: 70  LNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLAKLI 129

Query: 130 VTMACFYDRVKDLG-ENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSSDDVVALPY 189
           +T AC+ D+VKD   E  ++I+C+D + + CLHFS L  ADES M  V   SDDVVALPY
Sbjct: 130 ITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVVIDSDDVVALPY 189

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQVDG NPNLY   +DV++C+LPLFHIYSLN++L CG
Sbjct: 190 SSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAVLCCG 249

Query: 250 LRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVSSVRVLKSG 309
           LRAG  I+IMQKFDIV   +LI KYK++I P VPPI LAIAKS   +KYD+SSVR + SG
Sbjct: 250 LRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVMSG 309

Query: 310 GAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEACGTVVRNAE 369
            APLGKEL +AV+AKFP A L QGYGMTEAGPVL M LAFAKEP+++KS ACGTVVRNAE
Sbjct: 310 AAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAE 369

Query: 370 MKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGFIDDNN 429
           MKIVDPET +SLP N  GEICIRGDQIMKGYLND EST+ TID+EGWLHTGDIGFIDD++
Sbjct: 370 MKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDDDD 429

Query: 430 ELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPVAFVVEEKS 489
           ELFI+DRLKE+IK+K FQVAPAELEALL+THP + DAAV+ M D +AGEVPVAFVV    
Sbjct: 430 ELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRTN- 489

Query: 490 GASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLASG 543
               TTTEEE+K+F++KQV+FYK++ RVFFV+AIPK+PSGKILRK++RA +ASG
Sbjct: 490 --GFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKIASG 540

BLAST of CSPI07G19800 vs. TrEMBL
Match: A0A0A0K6Q5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430230 PE=4 SV=1)

HSP 1 Score: 1062.4 bits (2746), Expect = 1.9e-307
Identity = 541/545 (99.27%), Postives = 543/545 (99.63%), Query Frame = 1

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESNQTNEFIFRSKLP+IHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ
Sbjct: 1   MAFESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180
           AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300
           LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 360
           SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVK EAC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKFEAC 360

Query: 361 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETG SLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGVSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480
           IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 540
           AFVVEEKSGASATTTEEEVKEFIAKQVIFYK+LKRVFFVNAIPKAPSGKILRKEIRANLA
Sbjct: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKRLKRVFFVNAIPKAPSGKILRKEIRANLA 540

Query: 541 SGAYN 546
           SGAYN
Sbjct: 541 SGAYN 545

BLAST of CSPI07G19800 vs. TrEMBL
Match: A0A0A0K8H9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430240 PE=4 SV=1)

HSP 1 Score: 969.5 bits (2505), Expect = 1.7e-279
Identity = 489/545 (89.72%), Postives = 516/545 (94.68%), Query Frame = 1

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESN+TNEFIFRSKLP+IHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180
           AKAANAKLIVTMACFYDRVKDL ENGV+IVCVDF+ EGCLHFSVLSGADESL   VDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYY  +DVILCVLP FHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300
           LNSILLCGLR GAAIMIMQKFDIV+L QLI K++ISI+PIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 360
           SVRVLKSGGAPLGKEL +AV+ KFP AIL QGYGMTEAGPVL+MSLAFAKEPFQVK+ AC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETG+SLP NS+GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480
           IGF+DD+NELFI+DRLKELIKFK FQVAPAELEALLITHPKL DAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 540
           AFV++   GA    +EEEVK+FIAKQV+FYK+LKRVFFVNAIPKAPSGKILRKE+RA LA
Sbjct: 481 AFVMKANGGA---ISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA 540

Query: 541 SGAYN 546
           SGAYN
Sbjct: 541 SGAYN 542

BLAST of CSPI07G19800 vs. TrEMBL
Match: A0A0A0K658_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430250 PE=4 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 1.6e-277
Identity = 484/545 (88.81%), Postives = 513/545 (94.13%), Query Frame = 1

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESN+TNEFIFRSKLP+IHIPNHLPLHDYVFQNLSKFASRPCLINGATGD YTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180
           AKAANAKLIVTMACFYDRVKDL ENGV++VCVDF+ EGCLHFSVLSGADESL   VDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYY  DDVIL VLP FHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300
           LNSILLCGLR GAAI+IMQKFDIVSL QLI K++ISI+PIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 360
           SVRVLKSGGAPLGKEL +AV+ KFP A+L QGYGMTEAGPVL+MSLAFAKEPFQVK+ AC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETG+SLP NS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480
           IGF+DD++ELFI+DRLKELIKFK FQVAPAELEALLITHPKL DAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 540
           AFVV+   GA    TEEEVK+FI KQV+FYK+LKRVFFVNAIPKAPSGKILRKE+RA LA
Sbjct: 481 AFVVKANGGA---ITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA 540

Query: 541 SGAYN 546
           SGAYN
Sbjct: 541 SGAYN 542

BLAST of CSPI07G19800 vs. TrEMBL
Match: A0A089YQ16_BETPN (4-coumarate:CoA ligase 1 OS=Betula pendula PE=2 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 1.5e-227
Identity = 392/546 (71.79%), Postives = 463/546 (84.80%), Query Frame = 1

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MA ++ +  EFIFRSKLP+I+IP HLP+H Y F+N+SK  SRPCLING TG VYTY+DV+
Sbjct: 1   MAIQAKE-QEFIFRSKLPDIYIPKHLPIHSYCFENISKVGSRPCLINGLTGKVYTYYDVE 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTAR+VA+GL  LGI+KGDVVM LLPNSPEF F FLGASY GA+ TAANPF TA E++KQ
Sbjct: 61  LTARKVASGLSKLGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDL-GENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFS 180
           AK+ANAK++VT AC+YDRVKD   ENGVKI+C+D   E CLHFS L+ ADE+ ++ VD S
Sbjct: 121 AKSANAKIVVTQACYYDRVKDYTNENGVKIICIDSPPEDCLHFSELTKADENDVAEVDIS 180

Query: 181 SDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIY 240
            DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQVDG+NPNLYY  +DVILCVLPLFHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHIY 240

Query: 241 SLNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDV 300
           SLNS+ LCGLRAGA+I+I+ KF+IVSL QLI K+K++++PIVPPI LAI K  + +KYD+
Sbjct: 241 SLNSVFLCGLRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYDL 300

Query: 301 SSVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEA 360
           SSV++LKSGGAPLGKE+ E VKAKFP A+  QGYGMTEAGPVL M LAFAKEP +VKS A
Sbjct: 301 SSVKMLKSGGAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMEVKSGA 360

Query: 361 CGTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVDPETG+SLP N  GEICIRGDQIMKGY+ND E+T  TIDKEGWLHTG
Sbjct: 361 CGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHTG 420

Query: 421 DIGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVP 480
           DIG IDDN+ELFI+DRLKELIK+K FQVAPAELEALL+THP + DAAV+ M D  AGEVP
Sbjct: 421 DIGLIDDNDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEVP 480

Query: 481 VAFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANL 540
           VAFV        +  TE+E+K+F++KQV+FYK++ RVFF++ +PK+PSGKILRKE+RA L
Sbjct: 481 VAFVARSN---GSQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKL 540

Query: 541 ASGAYN 546
           A+G  N
Sbjct: 541 AAGFSN 542

BLAST of CSPI07G19800 vs. TrEMBL
Match: Q5S017_BETPL (4-coumarate:CoA ligase OS=Betula platyphylla PE=2 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 1.5e-227
Identity = 392/546 (71.79%), Postives = 463/546 (84.80%), Query Frame = 1

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MA ++ +  EFIFRSKLP+I+IP HLP+H Y F+N+SK  SRPCLING TG VYTY+DV+
Sbjct: 1   MAIQAKE-QEFIFRSKLPDIYIPKHLPIHSYCFENISKVGSRPCLINGLTGKVYTYYDVE 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTAR+VA+GL  LGI+KGDVVM LLPNSPEF F FLGASY GA+ TAANPF TA E++KQ
Sbjct: 61  LTARKVASGLSKLGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDL-GENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFS 180
           AK+ANAK++VT AC+YDRVKD   ENGVKI+C+D   E CLHFS L+ ADE+ ++ VD S
Sbjct: 121 AKSANAKIVVTQACYYDRVKDYTNENGVKIICIDSPPEDCLHFSELTKADENDVAEVDIS 180

Query: 181 SDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIY 240
            DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQVDG+NPNLYY  +DVILCVLPLFHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHIY 240

Query: 241 SLNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDV 300
           SLNS+ LCGLRAGA+I+I+ KF+IVSL QLI K+K++++PIVPPI LAI K  + +KYD+
Sbjct: 241 SLNSVFLCGLRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYDL 300

Query: 301 SSVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEA 360
           SSV++LKSGGAPLGKE+ E VKAKFP A+  QGYGMTEAGPVL M LAFAKEP +VKS A
Sbjct: 301 SSVKMLKSGGAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMEVKSGA 360

Query: 361 CGTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVDPETG+SLP N  GEICIRGDQIMKGY+ND E+T  TIDKEGWLHTG
Sbjct: 361 CGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHTG 420

Query: 421 DIGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVP 480
           DIG IDDN+ELFI+DRLKELIK+K FQVAPAELEALL+THP + DAAV+ M D  AGEVP
Sbjct: 421 DIGLIDDNDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEVP 480

Query: 481 VAFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANL 540
           VAFV        +  TE+E+K+F++KQV+FYK++ RVFF++ +PK+PSGKILRKE+RA L
Sbjct: 481 VAFVARSN---GSQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKL 540

Query: 541 ASGAYN 546
           A+G  N
Sbjct: 541 AAGFSN 542

BLAST of CSPI07G19800 vs. TAIR10
Match: AT3G21240.1 (AT3G21240.1 4-coumarate:CoA ligase 2)

HSP 1 Score: 731.1 bits (1886), Expect = 5.0e-211
Identity = 361/542 (66.61%), Postives = 448/542 (82.66%), Query Frame = 1

Query: 8   TNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRVA 67
           +N+ IFRS+LP+I+IPNHLPLHDY+F+N+S+FA++PCLING TG+VYTY DV +T+R++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  AGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAK 127
           AGLHNLG+K+ DVVM LLPNSPE V TFL AS+ GAI T+ANPF+T  EI+KQAKA+ AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 LIVTMACFYDRVKDLGENGVKIVCVDFSA--EGCLHFSVLSGADESLMSRVD--FSSDDV 187
           LIVT + + D++K+L  +GV IV  D  A  E CL FS L+ ++E  +  +    S +DV
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPEDV 197

Query: 188 VALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYSLNS 247
           VALP+SSGTTGLPKGVMLTHKGL+TSVAQQVDG+NPNLY+  DDVILCVLP+FHIY+LNS
Sbjct: 198 VALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNS 257

Query: 248 ILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVSSVR 307
           I+LC LR GA I+IM KF+I  L + I + K+++  +VPPI LAIAKS E EKYD+SSVR
Sbjct: 258 IMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVR 317

Query: 308 VLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEACGTV 367
           ++KSG APLGKEL +A+ AKFP A L QGYGMTEAGPVL MSL FAKEPF VKS ACGTV
Sbjct: 318 MVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTV 377

Query: 368 VRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGF 427
           VRNAEMKI+DP+TG SLP N  GEICIRG+QIMKGYLND  +T  TIDK+GWLHTGD+GF
Sbjct: 378 VRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGF 437

Query: 428 IDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPVAFV 487
           IDD++ELFI+DRLKELIK+K FQVAPAELE+LLI HP++ D AV+ M + +AGEVPVAFV
Sbjct: 438 IDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFV 497

Query: 488 VEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLASGA 546
           V  K    +  +E+E+K+F++KQV+FYK++ +VFF ++IPKAPSGKILRK++RA LA+G 
Sbjct: 498 VRSK---DSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGL 556

BLAST of CSPI07G19800 vs. TAIR10
Match: AT1G51680.1 (AT1G51680.1 4-coumarate:CoA ligase 1)

HSP 1 Score: 727.6 bits (1877), Expect = 5.6e-210
Identity = 364/547 (66.54%), Postives = 444/547 (81.17%), Query Frame = 1

Query: 5   SNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTAR 64
           +N  ++ IFRSKLP+I+IPNHL LHDY+FQN+S+FA++PCLING TG VYTY DV + +R
Sbjct: 17  NNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISR 76

Query: 65  RVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAA 124
           ++AA  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF+T  EIAKQAKA+
Sbjct: 77  QIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKAS 136

Query: 125 NAKLIVTMACFYDRVKDL-GENGVKIVCVDFSA-----EGCLHFSVLSGAD---ESLMSR 184
           N KLI+T A + D++K L  ++GV IVC+D +      EGCL F+ L+ +      ++  
Sbjct: 137 NTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDS 196

Query: 185 VDFSSDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPL 244
           V+ S DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQVDG+NPNLY+  DDVILCVLP+
Sbjct: 197 VEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPM 256

Query: 245 FHIYSLNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFE 304
           FHIY+LNSI+LCGLR GAAI+IM KF+I  L +LI + K+++ P+VPPI LAIAKS+E E
Sbjct: 257 FHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETE 316

Query: 305 KYDVSSVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQV 364
           KYD+SS+RV+KSG APLGKEL +AV AKFP A L QGYGMTEAGPVL MSL FAKEPF V
Sbjct: 317 KYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPV 376

Query: 365 KSEACGTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGW 424
           KS ACGTVVRNAEMKIVDP+TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GW
Sbjct: 377 KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGW 436

Query: 425 LHTGDIGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEA 484
           LHTGDIG IDD++ELFI+DRLKELIK+K FQVAPAELEALLI HP + D AV+ M +  A
Sbjct: 437 LHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAA 496

Query: 485 GEVPVAFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEI 543
           GEVPVAFVV+ K    +  +E++VK+F++KQV+FYK++ +VFF  +IPKAPSGKILRK++
Sbjct: 497 GEVPVAFVVKSK---DSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDL 556

BLAST of CSPI07G19800 vs. TAIR10
Match: AT3G21230.1 (AT3G21230.1 4-coumarate:CoA ligase 5)

HSP 1 Score: 654.1 bits (1686), Expect = 7.8e-188
Identity = 325/549 (59.20%), Postives = 420/549 (76.50%), Query Frame = 1

Query: 4   ESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKF----ASRPCLINGATGDVYTYHDV 63
           E   +++FIFRSKLP+I IPNHLPL DYVFQ  S      +S  C+I+GATG + TY DV
Sbjct: 20  EEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADV 79

Query: 64  QLTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAK 123
           Q   RR+AAG+H LGI+ GDVVM LLPNSPEF  +FL  +Y GA+ T ANPFYT  EIAK
Sbjct: 80  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 139

Query: 124 QAKAANAKLIVTMACFYDRVKDLGENGVKIVCVD---------FSAEGCLHFSVLSGADE 183
           QAKA+ AK+I+T  C  D++ +L  +GV IVC+D          S +GC+ F+ L+ ADE
Sbjct: 140 QAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADE 199

Query: 184 SLMSRVDFSSDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVIL 243
           + + +   S +D VA+PYSSGTTGLPKGVM+THKGL+TS+AQ+VDG+NPNL +  +DVIL
Sbjct: 200 TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVIL 259

Query: 244 CVLPLFHIYSLNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAK 303
           C LP+FHIY+L++++L  +R GAA++I+ +F++  + +LI +YK+++VP+ PP+ LA  K
Sbjct: 260 CFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIK 319

Query: 304 SAEFEKYDVSSVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAK 363
           S E E+YD+SSVR++ SG A L KEL +AV+ KFP AI  QGYGMTE+G V   SLAFAK
Sbjct: 320 SPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAK 379

Query: 364 EPFQVKSEACGTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTI 423
            PF+ KS ACGTV+RNAEMK+VD ETG SLP N +GEIC+RG Q+MKGYLND E+T RTI
Sbjct: 380 NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTI 439

Query: 424 DKEGWLHTGDIGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGM 483
           DK+GWLHTGDIGF+DD++E+FI+DRLKELIKFK +QVAPAELEALLI+HP + DAAV+ M
Sbjct: 440 DKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAM 499

Query: 484 PDVEAGEVPVAFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKI 540
            D  A EVPVAFV   +    +  TE++VK ++ KQV+ YK++K VFF+  IPKA SGKI
Sbjct: 500 KDEVADEVPVAFVARSQ---GSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKI 559

BLAST of CSPI07G19800 vs. TAIR10
Match: AT1G65060.1 (AT1G65060.1 4-coumarate:CoA ligase 3)

HSP 1 Score: 640.2 bits (1650), Expect = 1.2e-183
Identity = 323/530 (60.94%), Postives = 405/530 (76.42%), Query Frame = 1

Query: 12  IFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRVAAGLH 71
           IFRSKLP+I IPNHLPLH Y F+ LS  + +PCLI G+TG  YTY +  L  RRVA+GL+
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  NLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLIVT 131
            LGI+KGDV+M LL NS EFVF+F+GAS  GA+ T ANPFYT+ E+ KQ K++ AKLI+T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 MACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADES--LMSRVDFSSDDVVALPYS 191
            + + D++K+LGEN   I   + + E CL FS L   DE+      VD   DD  ALP+S
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALPFS 213

Query: 192 SGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYSLNSILLCGL 251
           SGTTGLPKGV+LTHK LITSVAQQVDG NPNLY + +DVILCVLPLFHIYSLNS+LL  L
Sbjct: 214 SGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSL 273

Query: 252 RAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVSSVRVLKSGG 311
           R+GA +++M KF+I +L  LI +++++I  +VPP+ +A+AK+     YD+SSVR + SG 
Sbjct: 274 RSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLSGA 333

Query: 312 APLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEACGTVVRNAEM 371
           APLGKEL ++++ + P AIL QGYGMTEAGPVL+MSL FAKEP   KS +CGTVVRNAE+
Sbjct: 334 APLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAEL 393

Query: 372 KIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGFIDDNNE 431
           K+V  ET  SL  N  GEICIRG QIMK YLND E+T  TID+EGWLHTGDIG++D+++E
Sbjct: 394 KVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDE 453

Query: 432 LFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPVAFVVEEKSG 491
           +FI+DRLKE+IKFK FQV PAELE+LLI H  + DAAV+   D  AGEVPVAFVV     
Sbjct: 454 IFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGN 513

Query: 492 ASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANL 540
                TEE+VKE++AKQV+FYK+L +VFFV +IPK+PSGKILRK+++A L
Sbjct: 514 ---DITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of CSPI07G19800 vs. TAIR10
Match: AT1G62940.1 (AT1G62940.1 acyl-CoA synthetase 5)

HSP 1 Score: 395.6 bits (1015), Expect = 5.0e-110
Identity = 213/545 (39.08%), Postives = 328/545 (60.18%), Query Frame = 1

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           M  +  + NE+IFRS  P++ IP+ L L ++V Q + ++      +   TG   TY DV 
Sbjct: 1   MESQKQEDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVV 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
              +R+A  L +LG++KG V++ +LPN  E+    LG    G + + ANP     EI KQ
Sbjct: 61  RDTKRLAKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESL--MSRVDF 180
            +A+ A+ I+T A  Y++VK LG     IV  +   EG +++  L  A +        + 
Sbjct: 121 VEASGARGIITDATNYEKVKSLGLP--VIVLGEEKIEGAVNWKDLLEAGDKCGDTDNEEI 180

Query: 181 SSDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHI 240
              D+ ALP+SSGTTGL KGVMLTH+ LI ++   + G    +   G  V L ++P FHI
Sbjct: 181 LQTDLCALPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMI--GQIVTLGLIPFFHI 240

Query: 241 YSLNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYD 300
           Y +  I    ++    ++ M ++D+      +  +++S  PIVPPI L + K+   +++D
Sbjct: 241 YGIVGICCATMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFD 300

Query: 301 VSSVRV--LKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVK 360
           +S +++  + +  APL  EL+ A +AKFP   + + YG+TE   +        K     K
Sbjct: 301 LSKLKLQSVMTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAK 360

Query: 361 SEACGTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWL 420
             + G ++ N E+K +DP+TG SLP+N++GE+C+R   +M+GY  + E T +TID++GWL
Sbjct: 361 RNSVGFILPNLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWL 420

Query: 421 HTGDIGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAG 480
           HTGDIG+IDD+ ++FI+DR+KELIK+K FQVAPAELEA+L+THP + D AV+ +PD EAG
Sbjct: 421 HTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAG 480

Query: 481 EVPVAFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIR 540
           E+P A VV       AT  EE++  F+A  V  YKK++ V FV++IPK+ SGKI+R+ +R
Sbjct: 481 EIPAACVV---INPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLR 538

Query: 541 ANLAS 542
             + S
Sbjct: 541 DKILS 538

BLAST of CSPI07G19800 vs. NCBI nr
Match: gi|449436228|ref|XP_004135895.1| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus])

HSP 1 Score: 1062.4 bits (2746), Expect = 2.7e-307
Identity = 541/545 (99.27%), Postives = 543/545 (99.63%), Query Frame = 1

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESNQTNEFIFRSKLP+IHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ
Sbjct: 1   MAFESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180
           AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300
           LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 360
           SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVK EAC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKFEAC 360

Query: 361 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETG SLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGVSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480
           IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 540
           AFVVEEKSGASATTTEEEVKEFIAKQVIFYK+LKRVFFVNAIPKAPSGKILRKEIRANLA
Sbjct: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKRLKRVFFVNAIPKAPSGKILRKEIRANLA 540

Query: 541 SGAYN 546
           SGAYN
Sbjct: 541 SGAYN 545

BLAST of CSPI07G19800 vs. NCBI nr
Match: gi|659122581|ref|XP_008461218.1| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 1021.9 bits (2641), Expect = 4.0e-295
Identity = 517/545 (94.86%), Postives = 531/545 (97.43%), Query Frame = 1

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESNQT+EFIFRSKLP+IHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVY+Y+DV 
Sbjct: 1   MAFESNQTHEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYYDVN 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180
            KAANAKLIVTMACFYDRVKDL ENGVKIVCVDFS EGCLHFSVLSGADESLM RVDFSS
Sbjct: 121 VKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFSVEGCLHFSVLSGADESLMPRVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300
           LNSILLCGLRAGAAI IMQKFDIVSLFQLIGKYK+SIVPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAITIMQKFDIVSLFQLIGKYKVSIVPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 360
           SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVK+EAC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAEAC 360

Query: 361 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETG+SLPENS GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPENSPGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480
           IGF+DDNNELFIIDRLKELIKFKTFQVAPAELEALLI HPKLRDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDNNELFIIDRLKELIKFKTFQVAPAELEALLIAHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 540
           AFVVEEKSGA   TTEEEVK+F+ KQV+FYK+LKRVFFVNAIPKAPSGKILRKE+RA LA
Sbjct: 481 AFVVEEKSGA---TTEEEVKQFVTKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA 540

Query: 541 SGAYN 546
           SGAYN
Sbjct: 541 SGAYN 542

BLAST of CSPI07G19800 vs. NCBI nr
Match: gi|778728825|ref|XP_004135894.2| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus])

HSP 1 Score: 969.5 bits (2505), Expect = 2.4e-279
Identity = 489/545 (89.72%), Postives = 516/545 (94.68%), Query Frame = 1

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESN+TNEFIFRSKLP+IHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180
           AKAANAKLIVTMACFYDRVKDL ENGV+IVCVDF+ EGCLHFSVLSGADESL   VDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYY  +DVILCVLP FHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300
           LNSILLCGLR GAAIMIMQKFDIV+L QLI K++ISI+PIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 360
           SVRVLKSGGAPLGKEL +AV+ KFP AIL QGYGMTEAGPVL+MSLAFAKEPFQVK+ AC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETG+SLP NS+GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480
           IGF+DD+NELFI+DRLKELIKFK FQVAPAELEALLITHPKL DAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 540
           AFV++   GA    +EEEVK+FIAKQV+FYK+LKRVFFVNAIPKAPSGKILRKE+RA LA
Sbjct: 481 AFVMKANGGA---ISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA 540

Query: 541 SGAYN 546
           SGAYN
Sbjct: 541 SGAYN 542

BLAST of CSPI07G19800 vs. NCBI nr
Match: gi|659122579|ref|XP_008461217.1| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 963.8 bits (2490), Expect = 1.3e-277
Identity = 486/545 (89.17%), Postives = 515/545 (94.50%), Query Frame = 1

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESN+TNEFIFRSKLP+IHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVM+LLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMHLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180
           AKAA+AKLIVTMACFYDRVKDLGENGVKIVCVDF+ EGCLHFSVLSGADESL   VDFSS
Sbjct: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYY  +DVILCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300
           LNSILLCGLR G+AI+I+QKFDIVSL QLI K++ISI+PIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 360
           SVRVLKSGGAPLGKEL +AV+ KFP AIL QGYGMTEAGPVL+MSLAFAKEPFQVK+ AC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETG+SLP NS+GEICIRG+QIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480
           IGF+D++NELFI+DRLKELIKFK FQVAPAELEALLITHPKL DAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 540
           AFVV+   GA    TEEEVK+FI KQV+FYK+LKRV FVNAIPKAPSGKILRKE+RA LA
Sbjct: 481 AFVVKAIGGA---ITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLA 540

Query: 541 SGAYN 546
           SGAYN
Sbjct: 541 SGAYN 542

BLAST of CSPI07G19800 vs. NCBI nr
Match: gi|778728828|ref|XP_004135893.2| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus])

HSP 1 Score: 963.0 bits (2488), Expect = 2.2e-277
Identity = 484/545 (88.81%), Postives = 513/545 (94.13%), Query Frame = 1

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESN+TNEFIFRSKLP+IHIPNHLPLHDYVFQNLSKFASRPCLINGATGD YTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180
           AKAANAKLIVTMACFYDRVKDL ENGV++VCVDF+ EGCLHFSVLSGADESL   VDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYY  DDVIL VLP FHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300
           LNSILLCGLR GAAI+IMQKFDIVSL QLI K++ISI+PIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 360
           SVRVLKSGGAPLGKEL +AV+ KFP A+L QGYGMTEAGPVL+MSLAFAKEPFQVK+ AC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETG+SLP NS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480
           IGF+DD++ELFI+DRLKELIKFK FQVAPAELEALLITHPKL DAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 540
           AFVV+   GA    TEEEVK+FI KQV+FYK+LKRVFFVNAIPKAPSGKILRKE+RA LA
Sbjct: 481 AFVVKANGGA---ITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA 540

Query: 541 SGAYN 546
           SGAYN
Sbjct: 541 SGAYN 542

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
4CL2_TOBAC2.3e-22170.954-coumarate--CoA ligase 2 OS=Nicotiana tabacum GN=4CL2 PE=1 SV=1[more]
4CL_VANPL7.3e-22070.724-coumarate--CoA ligase OS=Vanilla planifolia GN=4CL PE=3 SV=1[more]
4CL1_TOBAC1.1e-21869.064-coumarate--CoA ligase 1 OS=Nicotiana tabacum GN=4CL1 PE=2 SV=1[more]
4CL1_PETCR7.5e-21769.294-coumarate--CoA ligase 1 OS=Petroselinum crispum GN=4CL1 PE=2 SV=1[more]
4CL2_PETCR2.2e-21669.294-coumarate--CoA ligase 1 OS=Petroselinum crispum GN=4CL2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K6Q5_CUCSA1.9e-30799.27Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430230 PE=4 SV=1[more]
A0A0A0K8H9_CUCSA1.7e-27989.72Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430240 PE=4 SV=1[more]
A0A0A0K658_CUCSA1.6e-27788.81Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430250 PE=4 SV=1[more]
A0A089YQ16_BETPN1.5e-22771.794-coumarate:CoA ligase 1 OS=Betula pendula PE=2 SV=1[more]
Q5S017_BETPL1.5e-22771.794-coumarate:CoA ligase OS=Betula platyphylla PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G21240.15.0e-21166.61 4-coumarate:CoA ligase 2[more]
AT1G51680.15.6e-21066.54 4-coumarate:CoA ligase 1[more]
AT3G21230.17.8e-18859.20 4-coumarate:CoA ligase 5[more]
AT1G65060.11.2e-18360.94 4-coumarate:CoA ligase 3[more]
AT1G62940.15.0e-11039.08 acyl-CoA synthetase 5[more]
Match NameE-valueIdentityDescription
gi|449436228|ref|XP_004135895.1|2.7e-30799.27PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus][more]
gi|659122581|ref|XP_008461218.1|4.0e-29594.86PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo][more]
gi|778728825|ref|XP_004135894.2|2.4e-27989.72PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus][more]
gi|659122579|ref|XP_008461217.1|1.3e-27789.17PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo][more]
gi|778728828|ref|XP_004135893.2|2.2e-27788.81PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000873AMP-dep_Synth/Lig
IPR020845AMP-binding_CS
IPR025110AMP-bd_C
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009805 coumarin biosynthetic process
biological_process GO:0009809 lignin biosynthetic process
biological_process GO:0009811 stilbene biosynthetic process
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016207 4-coumarate-CoA ligase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0016874 ligase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G19800.1CSPI07G19800.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 35..442
score: 2.7E
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 186..197
scor
IPR025110AMP-binding enzyme C-terminal domainPFAMPF13193AMP-binding_Ccoord: 451..529
score: 2.4
NoneNo IPR availableGENE3DG3DSA:2.30.38.10coord: 360..432
score: 1.1
NoneNo IPR availableGENE3DG3DSA:3.30.300.30coord: 439..540
score: 7.8
NoneNo IPR availableGENE3DG3DSA:3.40.50.980coord: 28..191
score: 4.0E-32coord: 192..347
score: 1.8
NoneNo IPR availablePANTHERPTHR24096FAMILY NOT NAMEDcoord: 4..537
score:
NoneNo IPR availablePANTHERPTHR24096:SF1494-COUMARATE--COA LIGASE 1-RELATEDcoord: 4..537
score:
NoneNo IPR availableunknownSSF56801Acetyl-CoA synthetase-likecoord: 12..540
score: 5.63E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CSPI07G19800CSPI03G30180Wild cucumber (PI 183967)cpicpiB142
The following block(s) are covering this gene:
GeneOrganismBlock
CSPI07G19800Cucurbita pepo (Zucchini)cpecpiB163
CSPI07G19800Cucurbita pepo (Zucchini)cpecpiB344
CSPI07G19800Silver-seed gourdcarcpiB0391
CSPI07G19800Silver-seed gourdcarcpiB0489