CmaCh08G004800 (gene) Cucurbita maxima (Rimu)

NameCmaCh08G004800
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionWRKY transcription factor, putative
LocationCma_Chr08 : 2725379 .. 2726821 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AGCCTTATTTCACCGGCTAGGGTTTATGGAGCTCGATTTAATTTGGTCGCAATTTGATATGTTTTCGACGTCGTCTTCCCCAATTCAAAACCCCGTTATCGATCCCGAGAATTTGGAGATTTTGAATGCATGGCTTACATCGTCCAGTCAGCTCATCAATGACGAGTTATCAGAGTTGAATTTAGAAGAGCAGAAACCTCGACAAAAAACTGTTAAATCTAACCCCTTGTTAGTGTTTTTTTTTTCTTTTTAATTCAACAATATTTAGAGGTAAATATAATCACTTTATTGATGTATTTAAAATATTAAATTTAGGTCGAAAACAAAGAAGACGAATCAAAAGAAGAAAGAGCAAGAACAACGATACATGTTCATGACGAAGAGCAAAGTTGATCATTTAGAAGATGGATATAGATGGAGAAAGTACGGGCAAAAAGTTGTAAAAAATAGCCCTTATCCTAGGTAATATCGTTGTACTCAAATTACTATTATACCCTAAATTTTATGGTGCATAGACGTTTCTAACATGTTTTTTTCTTTGTGTAGGAGTTATTATCGGTGTACTAGTGTAGCATGTAACGTAAAGAAACGAGTGGAGAGATGCTTGAAAGATCCCATAATTGTTATCACAACTTACGAAGGCCAACACACTCACTCTACCCCTGTCATGGCTCGATCAACCTTCTTTCCTCTGCCTATCTCTACCTCTCTCTACGAGGGTTCCACATCGTTCACCACCACCACTCCCCATCTCTTTCACAACCAAAACGTTCATAATTTAAATTTTGCCCGTCACCCTAATGACTTTGTGGCACCGTCATTTCATCACGAGCGATTGCCACAACACATTGCCTCCTTCAATCCACCTACTCATTTATTTGCTTCTACTACTAATTATGAGTTACTTCAAGATGTTAGTTGGTCGACATGATGTATTGTAACAACCCAAGTCCGCTACTACTAGATATTATCTGCTTTGACCCATTACGTTTCGTCATCATCTTCATGATTTTAAAACGCGTCGATTAGGTGATTTCACGAATGTTTCGTTCCCCTCTCCAACTACTATAGTTGTTTTATGTAATAATTTTTTAATAATTATTATTATGCGAACCTCAAATAATTTATTTATAACATATTTGTTTCCCCAAAAACAGAGATCTTTCTTCTAATTTATGAAACTATTTTTTAATTCAACAAAAGAATTTACTAAAAAAAAAAAACTAAATTTACTCATAAAAAAATTCAATTAATCCCCCATAAATGAAAAAAGAAAAAAAGAAAACCATTTCCGGCCCAGAAATTTACAAATGTGACCGACGATTCAGAATCAAGTTTCATGGGAGTTACCGTTTCCGTTGGCTGAAGGCGAATGCGAATGCCATCCCAGGATTGATTCAATCGTCGGTCGCCATCTCCGATGAACACAAATTCGATCATTAA

mRNA sequence

AGCCTTATTTCACCGGCTAGGGTTTATGGAGCTCGATTTAATTTGGTCGCAATTTGATATGTTTTCGACGTCGTCTTCCCCAATTCAAAACCCCGTTATCGATCCCGAGAATTTGGAGATTTTGAATGCATGGCTTACATCGTCCAGTCAGCTCATCAATGACGAGTTATCAGAGTTGAATTTAGAAGAGCAGAAACCTCGACAAAAAACTGTTAAATCTAACCCCTTGTCGAAAACAAAGAAGACGAATCAAAAGAAGAAAGAGCAAGAACAACGATACATGTTCATGACGAAGAGCAAAGTTGATCATTTAGAAGATGGATATAGATGGAGAAAGTACGGGCAAAAAGTTGTAAAAAATAGCCCTTATCCTAGGAGTTATTATCGGTGTACTAGTGTAGCATGTAACGTAAAGAAACGAGTGGAGAGATGCTTGAAAGATCCCATAATTGTTATCACAACTTACGAAGGCCAACACACTCACTCTACCCCTGTCATGGCTCGATCAACCTTCTTTCCTCTGCCTATCTCTACCTCTCTCTACGAGGGTTCCACATCGTTCACCACCACCACTCCCCATCTCTTTCACAACCAAAACGTTCATAATTTAAATTTTGCCCGTCACCCTAATGACTTTGTGGCACCAATCAAGTTTCATGGGAGTTACCGTTTCCGTTGGCTGAAGGCGAATGCGAATGCCATCCCAGGATTGATTCAATCGTCGGTCGCCATCTCCGATGAACACAAATTCGATCATTAA

Coding sequence (CDS)

ATGGAGCTCGATTTAATTTGGTCGCAATTTGATATGTTTTCGACGTCGTCTTCCCCAATTCAAAACCCCGTTATCGATCCCGAGAATTTGGAGATTTTGAATGCATGGCTTACATCGTCCAGTCAGCTCATCAATGACGAGTTATCAGAGTTGAATTTAGAAGAGCAGAAACCTCGACAAAAAACTGTTAAATCTAACCCCTTGTCGAAAACAAAGAAGACGAATCAAAAGAAGAAAGAGCAAGAACAACGATACATGTTCATGACGAAGAGCAAAGTTGATCATTTAGAAGATGGATATAGATGGAGAAAGTACGGGCAAAAAGTTGTAAAAAATAGCCCTTATCCTAGGAGTTATTATCGGTGTACTAGTGTAGCATGTAACGTAAAGAAACGAGTGGAGAGATGCTTGAAAGATCCCATAATTGTTATCACAACTTACGAAGGCCAACACACTCACTCTACCCCTGTCATGGCTCGATCAACCTTCTTTCCTCTGCCTATCTCTACCTCTCTCTACGAGGGTTCCACATCGTTCACCACCACCACTCCCCATCTCTTTCACAACCAAAACGTTCATAATTTAAATTTTGCCCGTCACCCTAATGACTTTGTGGCACCAATCAAGTTTCATGGGAGTTACCGTTTCCGTTGGCTGAAGGCGAATGCGAATGCCATCCCAGGATTGATTCAATCGTCGGTCGCCATCTCCGATGAACACAAATTCGATCATTAA

Protein sequence

MELDLIWSQFDMFSTSSSPIQNPVIDPENLEILNAWLTSSSQLINDELSELNLEEQKPRQKTVKSNPLSKTKKTNQKKKEQEQRYMFMTKSKVDHLEDGYRWRKYGQKVVKNSPYPRSYYRCTSVACNVKKRVERCLKDPIIVITTYEGQHTHSTPVMARSTFFPLPISTSLYEGSTSFTTTTPHLFHNQNVHNLNFARHPNDFVAPIKFHGSYRFRWLKANANAIPGLIQSSVAISDEHKFDH
BLAST of CmaCh08G004800 vs. Swiss-Prot
Match: WRK23_ARATH (Probable WRKY transcription factor 23 OS=Arabidopsis thaliana GN=WRKY23 PE=2 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 6.1e-33
Identity = 82/158 (51.90%), Postives = 108/158 (68.35%), Query Frame = 1

Query: 22  NPVIDPENLEILNAWLTSSSQLINDELSEL-NLEEQKPRQKTVKSNPLSKTK-KTNQKKK 81
           NP   P +  I +A    SS+ +N+E  +  + EE+    +  KS+   + K K N +K+
Sbjct: 101 NPPATPNSSSISSA----SSEALNEEKPKTEDNEEEGGEDQQEKSHTKKQLKAKKNNQKR 160

Query: 82  EQEQRYMFMTKSKVDHLEDGYRWRKYGQKVVKNSPYPRSYYRCTSVACNVKKRVERCLKD 141
           ++E R  FMTKS+VDHLEDGYRWRKYGQK VKNSP+PRSYYRCT+ +CNVKKRVER  +D
Sbjct: 161 QREARVAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTTASCNVKKRVERSFRD 220

Query: 142 PIIVITTYEGQHTHSTPVMARSTFFPLPISTSLYEGST 178
           P  V+TTYEGQHTH +P+ +R      PIST  + GS+
Sbjct: 221 PSTVVTTYEGQHTHISPLTSR------PISTGGFFGSS 248

BLAST of CmaCh08G004800 vs. Swiss-Prot
Match: WRK71_ARATH (Probable WRKY transcription factor 71 OS=Arabidopsis thaliana GN=WRKY71 PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 5.7e-31
Identity = 84/163 (51.53%), Postives = 99/163 (60.74%), Query Frame = 1

Query: 13  FSTSSSPIQNPVIDPENLEILNAWLTSSSQLI-------------NDELSELNLEEQKPR 72
           FS  SSP Q+ V    N   LN  LTS+S ++             ND+  ++   E    
Sbjct: 42  FSPYSSPFQS-VSPSVNNPYLN--LTSNSPVVSSSSNEGEPKENTNDKSDQMEDNEGDLH 101

Query: 73  QKTVKSNPLSKTKKTNQKKKEQEQRYMFMTKSKVDHLEDGYRWRKYGQKVVKNSPYPRSY 132
                S  L+K  K   +KKE+E R  FMTKS++DHLEDGYRWRKYGQK VKNSPYPRSY
Sbjct: 102 GVGESSKQLTKQGKKKGEKKEREVRVAFMTKSEIDHLEDGYRWRKYGQKAVKNSPYPRSY 161

Query: 133 YRCTSVACNVKKRVERCLKDPIIVITTYEGQHTHSTPVMARST 163
           YRCT+  CNVKKRVER  +DP IVITTYEG+H H  P   R T
Sbjct: 162 YRCTTQKCNVKKRVERSFQDPSIVITTYEGKHNHPIPSTLRGT 201

BLAST of CmaCh08G004800 vs. Swiss-Prot
Match: WRKY8_ARATH (Probable WRKY transcription factor 8 OS=Arabidopsis thaliana GN=WRKY8 PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 9.7e-31
Identity = 76/140 (54.29%), Postives = 91/140 (65.00%), Query Frame = 1

Query: 64  KSNPLSKTKKTNQKKKEQEQRYMFMTKSKVDHLEDGYRWRKYGQKVVKNSPYPRSYYRCT 123
           +S  + KTKK  +KKKE   R  FMTK++VDHLEDGYRWRKYGQK VKNSPYPRSYYRCT
Sbjct: 152 RSQKVVKTKKKEEKKKEP--RVSFMTKTEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCT 211

Query: 124 SVACNVKKRVERCLKDPIIVITTYEGQHTHSTPVMARSTFFPLPISTSLYEGSTS----- 183
           +  CNVKKRVER  +DP +VITTYE QH H  P   R+  F    + S Y  S+S     
Sbjct: 212 TQKCNVKKRVERSYQDPTVVITTYESQHNHPIPTNRRTAMFS-GTTASDYNPSSSPIFSD 271

Query: 184 FTTTTPHLFHNQNVHNLNFA 199
               TP  F N ++  + +A
Sbjct: 272 LIINTPRSFSNDDLFRVPYA 288

BLAST of CmaCh08G004800 vs. Swiss-Prot
Match: WRK28_ARATH (Probable WRKY transcription factor 28 OS=Arabidopsis thaliana GN=WRKY28 PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.7e-30
Identity = 84/168 (50.00%), Postives = 100/168 (59.52%), Query Frame = 1

Query: 14  STSSSPIQNPVIDPE-NLEILNAWLT-----------SSSQLINDELSELNLEEQKPRQK 73
           S SSS + N  ID E N ++ N  +            S S   + E      +  K R+K
Sbjct: 71  SPSSSEVFNSSIDQEPNRDVTNDVINGGACNETETRVSPSNSSSSEADHPGEDSGKSRRK 130

Query: 74  TVK-------SNPLSKTKKTNQKKKEQEQRYMFMTKSKVDHLEDGYRWRKYGQKVVKNSP 133
                     S  + KTKKT + KK++E R  FMTKS+VDHLEDGYRWRKYGQK VKNSP
Sbjct: 131 RELVGEEDQISKKVGKTKKT-EVKKQREPRVSFMTKSEVDHLEDGYRWRKYGQKAVKNSP 190

Query: 134 YPRSYYRCTSVACNVKKRVERCLKDPIIVITTYEGQHTHSTPVMARST 163
           YPRSYYRCT+  CNVKKRVER  +DP +VITTYEGQH H  P   R +
Sbjct: 191 YPRSYYRCTTQKCNVKKRVERSFQDPTVVITTYEGQHNHPIPTNLRGS 237

BLAST of CmaCh08G004800 vs. Swiss-Prot
Match: WRK48_ARATH (Probable WRKY transcription factor 48 OS=Arabidopsis thaliana GN=WRKY48 PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 3.7e-30
Identity = 88/214 (41.12%), Postives = 120/214 (56.07%), Query Frame = 1

Query: 23  PVIDPENLEILNAWLTS---------SSQLIND-----ELSELNLEEQKPRQKTVKSNPL 82
           P+   E+ E++N   TS         S++  ND     E++  + EE   +Q+   + P 
Sbjct: 134 PLPQAESSEVVNTTPTSPNSTSVSSSSNEAANDNNSGKEVTVKDQEEGDQQQEQKGTKPQ 193

Query: 83  SKTKKTNQKKKEQEQRYMFMTKSKVDHLEDGYRWRKYGQKVVKNSPYPRSYYRCTSVACN 142
            K KK NQ KK +E R+ F+TKS +D+L+DGYRWRKYGQK VKNSPYPRSYYRCT+V C 
Sbjct: 194 LKAKKKNQ-KKAREARFAFLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCG 253

Query: 143 VKKRVERCLKDPIIVITTYEGQHTHSTPVMARSTF----FPLPISTSLYEGSTSFTTTTP 202
           VKKRVER   DP IV+TTYEGQHTH  P+  R        P+    +    S+SF+   P
Sbjct: 254 VKKRVERSSDDPSIVMTTYEGQHTHPFPMTPRGHIGMLTSPILDHGATTASSSSFSIPQP 313

Query: 203 -----------HLFHNQNVHNLNFARHPNDFVAP 208
                      ++++N ++  +N       FV P
Sbjct: 314 RYLLTQHHQPYNMYNNNSLSMINRRSSDGTFVNP 346

BLAST of CmaCh08G004800 vs. TrEMBL
Match: E7CEY6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=WRKY52 PE=2 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 5.3e-52
Identity = 124/208 (59.62%), Postives = 147/208 (70.67%), Query Frame = 1

Query: 8   SQFDMFSTSSSPIQNPVI-DPENLEILNAWLTS----------SSQLINDELS-ELNLE- 67
           SQFD+F TSS  + + +I +PENLEI N W T+          SS+++N EL+ E NLE 
Sbjct: 39  SQFDVFETSSPSLSSSLISNPENLEIWNQWPTTPNYSSSISSTSSEIVNGELTTEPNLEG 98

Query: 68  -EQKP-RQKTVKSNPLSKTKKTNQKKKEQEQRYMFMTKSKVDHLEDGYRWRKYGQKVVKN 127
            EQK  +Q TVK++   KTKK + KKK  E R+ FMTKS+VDHLEDGYRWRKYGQK VKN
Sbjct: 99  GEQKQDQQPTVKADKQLKTKKRSPKKKGAEPRFAFMTKSEVDHLEDGYRWRKYGQKAVKN 158

Query: 128 SPYPRSYYRCTSVACNVKKRVERCLKDPIIVITTYEGQHTHSTPVMARSTFFPLPISTSL 187
           SP+PRSYYRCTSVACNVKKRVERCL+DP IV+TTYEGQHTH +P+MARSTFFP PIS +L
Sbjct: 159 SPHPRSYYRCTSVACNVKKRVERCLQDPSIVVTTYEGQHTHPSPIMARSTFFPPPISATL 218

Query: 188 YEGSTSFTTTTPHLFHNQNVHNLNFARH 201
           Y             +  QN HN N   H
Sbjct: 219 YND-----------YSIQNSHNSNVMSH 235

BLAST of CmaCh08G004800 vs. TrEMBL
Match: A0A0D2P7Q0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G121200 PE=4 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.1e-36
Identity = 92/194 (47.42%), Postives = 128/194 (65.98%), Query Frame = 1

Query: 38  TSSSQLINDELSELNLEEQKPRQKTVKSNPLSKTKKTNQKKKEQEQRYMFMTKSKVDHLE 97
           ++SS+ ++DE ++L+ +E+  R    K+    K KKTNQK+ ++E R+ FMTKS+VDHLE
Sbjct: 104 SASSEAVHDEPAKLDDQEEDQR----KTKKQLKPKKTNQKR-QREPRFAFMTKSEVDHLE 163

Query: 98  DGYRWRKYGQKVVKNSPYPRSYYRCTSVACNVKKRVERCLKDPIIVITTYEGQHTHSTPV 157
           DGYRWRKYGQK VKNSP+PRSYYRCT+++CNVKKRVERC  DP IV+TTYEGQHTH +PV
Sbjct: 164 DGYRWRKYGQKAVKNSPFPRSYYRCTTISCNVKKRVERCFSDPSIVVTTYEGQHTHPSPV 223

Query: 158 MARSTFFPLPISTSLYEGSTSFT-TTTPHLFHNQNVHNLNFARHPNDFVAPIKF--HGSY 217
           M R       +++++   S   +  TTP  +H       +F +   D ++P+ F  +GS 
Sbjct: 224 MPRPNLVGSHLNSAISAASFGMSMQTTPSHYHQ------HFQQPFTDNLSPLNFGHNGSL 283

Query: 218 RFRWLKANANAIPG 229
              +L       PG
Sbjct: 284 NATFLHQKRFCTPG 286

BLAST of CmaCh08G004800 vs. TrEMBL
Match: D7U2K4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0005g01710 PE=4 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.8e-36
Identity = 93/197 (47.21%), Postives = 126/197 (63.96%), Query Frame = 1

Query: 16  SSSPIQNPVIDPENLEILNAWLTSSSQLINDELSELNLEEQKPRQKTVKSNPLSKTKKTN 75
           +SS + NP   P +        +S S   + E     +EE++  ++  K+    K KKT 
Sbjct: 76  ASSDVVNPPATPNS--------SSVSSASSHEQGSKAVEEEEEDEEEKKTKKELKPKKTT 135

Query: 76  QKKKEQEQRYMFMTKSKVDHLEDGYRWRKYGQKVVKNSPYPRSYYRCTSVACNVKKRVER 135
            +K+++E R+ FMTKS+VDHLEDGYRWRKYGQK VKNSP+PRSYYRCTS +CNVKKRVER
Sbjct: 136 SQKRQREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSASCNVKKRVER 195

Query: 136 CLKDPIIVITTYEGQHTHSTPVMARSTFFPLPISTSLYEGSTSFTTTTPHLFHNQNVHNL 195
           C KDP IV+TTYEGQHTH +P+M R+   P  I+T+ + G    TT   HL    ++H+ 
Sbjct: 196 CFKDPAIVVTTYEGQHTHPSPIMPRAN--PSSIATT-FAGPRLQTTPFQHL----HLHHH 255

Query: 196 NFARHPNDFVAPIKFHG 213
              +HP+    P+  +G
Sbjct: 256 QQQQHPSFNDVPLLNYG 257

BLAST of CmaCh08G004800 vs. TrEMBL
Match: K4EQ50_PANQU (WRKY28 OS=Panax quinquefolius PE=2 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.2e-35
Identity = 95/192 (49.48%), Postives = 121/192 (63.02%), Query Frame = 1

Query: 28  ENLEILNAWLTSSSQLIND------ELSELNLEEQKPRQKTVKSNPLSKTKKTNQKKKEQ 87
           ++ E+LN   T +S  ++       E S++ + EQ+   +  K+    K KKT+QKKK++
Sbjct: 79  DSSEVLNTPATPNSSSVSSVSSGAPEQSKIEVNEQQEGHQQHKTKQQLKAKKTSQKKKQR 138

Query: 88  EQRYMFMTKSKVDHLEDGYRWRKYGQKVVKNSPYPRSYYRCTSVACNVKKRVERCLKDPI 147
           E R+ FMTKS+VDHLEDGYRWRKYGQK VKNSP+PRSYYRCTS +CNVKKRVER  +DP 
Sbjct: 139 EPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSSSCNVKKRVERSFEDPS 198

Query: 148 IVITTYEGQHTHSTPVMARS---TFFPLPISTSLYEGSTSFTTTTPHLFHNQNVHNLNFA 207
           IV+TTYEGQHTH +PVM R      FP P S     GS  F     HL    N+ + +  
Sbjct: 199 IVVTTYEGQHTHQSPVMPRGISPAGFP-PGSGISAAGSAGF-----HLPVQMNLLSHHGQ 258

Query: 208 RHPNDFVAPIKF 211
            H N+   P  F
Sbjct: 259 PHFNNLAVPFNF 264

BLAST of CmaCh08G004800 vs. TrEMBL
Match: A0A140H8K6_MANES (WRKY transcription factor 2 OS=Manihot esculenta PE=2 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.6e-35
Identity = 93/168 (55.36%), Postives = 114/168 (67.86%), Query Frame = 1

Query: 8   SQFDMFSTSSS---PIQNPVIDP-ENLEILNAWLT--------SSSQLINDELSELNLEE 67
           S FD+    S       NPV+   E+ E LN   T        +SS+ +NDEL ++   E
Sbjct: 58  SMFDVLQVPSMVQPAASNPVVTKVESPEALNQPATPNSSSISSASSEALNDELVKVADNE 117

Query: 68  QKPRQKTVKSNPLSKTKKTNQKKKEQEQRYMFMTKSKVDHLEDGYRWRKYGQKVVKNSPY 127
           ++ +QKT K     K KKTNQK+ ++E R+ FMTKS+VDHLEDGYRWRKYGQK VKNSP+
Sbjct: 118 EEEQQKTKKE---LKPKKTNQKR-QREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPF 177

Query: 128 PRSYYRCTSVACNVKKRVERCLKDPIIVITTYEGQHTHSTPVMARSTF 164
           PRSYYRCTS +CNVKKRVER   DP IV+TTYEGQHTH + VM R +F
Sbjct: 178 PRSYYRCTSASCNVKKRVERSFSDPSIVVTTYEGQHTHPSAVMTRPSF 221

BLAST of CmaCh08G004800 vs. TAIR10
Match: AT2G47260.1 (AT2G47260.1 WRKY DNA-binding protein 23)

HSP 1 Score: 142.5 bits (358), Expect = 3.4e-34
Identity = 82/158 (51.90%), Postives = 108/158 (68.35%), Query Frame = 1

Query: 22  NPVIDPENLEILNAWLTSSSQLINDELSEL-NLEEQKPRQKTVKSNPLSKTK-KTNQKKK 81
           NP   P +  I +A    SS+ +N+E  +  + EE+    +  KS+   + K K N +K+
Sbjct: 101 NPPATPNSSSISSA----SSEALNEEKPKTEDNEEEGGEDQQEKSHTKKQLKAKKNNQKR 160

Query: 82  EQEQRYMFMTKSKVDHLEDGYRWRKYGQKVVKNSPYPRSYYRCTSVACNVKKRVERCLKD 141
           ++E R  FMTKS+VDHLEDGYRWRKYGQK VKNSP+PRSYYRCT+ +CNVKKRVER  +D
Sbjct: 161 QREARVAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTTASCNVKKRVERSFRD 220

Query: 142 PIIVITTYEGQHTHSTPVMARSTFFPLPISTSLYEGST 178
           P  V+TTYEGQHTH +P+ +R      PIST  + GS+
Sbjct: 221 PSTVVTTYEGQHTHISPLTSR------PISTGGFFGSS 248

BLAST of CmaCh08G004800 vs. TAIR10
Match: AT1G29860.1 (AT1G29860.1 WRKY DNA-binding protein 71)

HSP 1 Score: 136.0 bits (341), Expect = 3.2e-32
Identity = 84/163 (51.53%), Postives = 99/163 (60.74%), Query Frame = 1

Query: 13  FSTSSSPIQNPVIDPENLEILNAWLTSSSQLI-------------NDELSELNLEEQKPR 72
           FS  SSP Q+ V    N   LN  LTS+S ++             ND+  ++   E    
Sbjct: 42  FSPYSSPFQS-VSPSVNNPYLN--LTSNSPVVSSSSNEGEPKENTNDKSDQMEDNEGDLH 101

Query: 73  QKTVKSNPLSKTKKTNQKKKEQEQRYMFMTKSKVDHLEDGYRWRKYGQKVVKNSPYPRSY 132
                S  L+K  K   +KKE+E R  FMTKS++DHLEDGYRWRKYGQK VKNSPYPRSY
Sbjct: 102 GVGESSKQLTKQGKKKGEKKEREVRVAFMTKSEIDHLEDGYRWRKYGQKAVKNSPYPRSY 161

Query: 133 YRCTSVACNVKKRVERCLKDPIIVITTYEGQHTHSTPVMARST 163
           YRCT+  CNVKKRVER  +DP IVITTYEG+H H  P   R T
Sbjct: 162 YRCTTQKCNVKKRVERSFQDPSIVITTYEGKHNHPIPSTLRGT 201

BLAST of CmaCh08G004800 vs. TAIR10
Match: AT5G46350.1 (AT5G46350.1 WRKY DNA-binding protein 8)

HSP 1 Score: 135.2 bits (339), Expect = 5.5e-32
Identity = 76/140 (54.29%), Postives = 91/140 (65.00%), Query Frame = 1

Query: 64  KSNPLSKTKKTNQKKKEQEQRYMFMTKSKVDHLEDGYRWRKYGQKVVKNSPYPRSYYRCT 123
           +S  + KTKK  +KKKE   R  FMTK++VDHLEDGYRWRKYGQK VKNSPYPRSYYRCT
Sbjct: 152 RSQKVVKTKKKEEKKKEP--RVSFMTKTEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCT 211

Query: 124 SVACNVKKRVERCLKDPIIVITTYEGQHTHSTPVMARSTFFPLPISTSLYEGSTS----- 183
           +  CNVKKRVER  +DP +VITTYE QH H  P   R+  F    + S Y  S+S     
Sbjct: 212 TQKCNVKKRVERSYQDPTVVITTYESQHNHPIPTNRRTAMFS-GTTASDYNPSSSPIFSD 271

Query: 184 FTTTTPHLFHNQNVHNLNFA 199
               TP  F N ++  + +A
Sbjct: 272 LIINTPRSFSNDDLFRVPYA 288

BLAST of CmaCh08G004800 vs. TAIR10
Match: AT4G18170.1 (AT4G18170.1 WRKY DNA-binding protein 28)

HSP 1 Score: 134.4 bits (337), Expect = 9.3e-32
Identity = 84/168 (50.00%), Postives = 100/168 (59.52%), Query Frame = 1

Query: 14  STSSSPIQNPVIDPE-NLEILNAWLT-----------SSSQLINDELSELNLEEQKPRQK 73
           S SSS + N  ID E N ++ N  +            S S   + E      +  K R+K
Sbjct: 71  SPSSSEVFNSSIDQEPNRDVTNDVINGGACNETETRVSPSNSSSSEADHPGEDSGKSRRK 130

Query: 74  TVK-------SNPLSKTKKTNQKKKEQEQRYMFMTKSKVDHLEDGYRWRKYGQKVVKNSP 133
                     S  + KTKKT + KK++E R  FMTKS+VDHLEDGYRWRKYGQK VKNSP
Sbjct: 131 RELVGEEDQISKKVGKTKKT-EVKKQREPRVSFMTKSEVDHLEDGYRWRKYGQKAVKNSP 190

Query: 134 YPRSYYRCTSVACNVKKRVERCLKDPIIVITTYEGQHTHSTPVMARST 163
           YPRSYYRCT+  CNVKKRVER  +DP +VITTYEGQH H  P   R +
Sbjct: 191 YPRSYYRCTTQKCNVKKRVERSFQDPTVVITTYEGQHNHPIPTNLRGS 237

BLAST of CmaCh08G004800 vs. TAIR10
Match: AT5G49520.1 (AT5G49520.1 WRKY DNA-binding protein 48)

HSP 1 Score: 133.3 bits (334), Expect = 2.1e-31
Identity = 88/214 (41.12%), Postives = 120/214 (56.07%), Query Frame = 1

Query: 23  PVIDPENLEILNAWLTS---------SSQLIND-----ELSELNLEEQKPRQKTVKSNPL 82
           P+   E+ E++N   TS         S++  ND     E++  + EE   +Q+   + P 
Sbjct: 134 PLPQAESSEVVNTTPTSPNSTSVSSSSNEAANDNNSGKEVTVKDQEEGDQQQEQKGTKPQ 193

Query: 83  SKTKKTNQKKKEQEQRYMFMTKSKVDHLEDGYRWRKYGQKVVKNSPYPRSYYRCTSVACN 142
            K KK NQ KK +E R+ F+TKS +D+L+DGYRWRKYGQK VKNSPYPRSYYRCT+V C 
Sbjct: 194 LKAKKKNQ-KKAREARFAFLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCG 253

Query: 143 VKKRVERCLKDPIIVITTYEGQHTHSTPVMARSTF----FPLPISTSLYEGSTSFTTTTP 202
           VKKRVER   DP IV+TTYEGQHTH  P+  R        P+    +    S+SF+   P
Sbjct: 254 VKKRVERSSDDPSIVMTTYEGQHTHPFPMTPRGHIGMLTSPILDHGATTASSSSFSIPQP 313

Query: 203 -----------HLFHNQNVHNLNFARHPNDFVAP 208
                      ++++N ++  +N       FV P
Sbjct: 314 RYLLTQHHQPYNMYNNNSLSMINRRSSDGTFVNP 346

BLAST of CmaCh08G004800 vs. NCBI nr
Match: gi|659101173|ref|XP_008451465.1| (PREDICTED: probable WRKY transcription factor 23 [Cucumis melo])

HSP 1 Score: 213.8 bits (543), Expect = 3.4e-52
Identity = 120/207 (57.97%), Postives = 145/207 (70.05%), Query Frame = 1

Query: 8   SQFDMFSTSSSPIQNPVI-DPENLEILNAWLTS----------SSQLINDELSELNLE-- 67
           SQFD+F TSS  + + +I +PENLEI N W T+          SS+++N EL+E NLE  
Sbjct: 92  SQFDVFETSSPSLSSSLISNPENLEIWNQWPTTPNYSSSISSTSSEIVNGELTEPNLEGG 151

Query: 68  -EQKPRQKTVKSNPLSKTKKTNQKKKEQEQRYMFMTKSKVDHLEDGYRWRKYGQKVVKNS 127
            E++ RQ TVK++   KTKK + KKK  E R+ FMTKS+VDHLEDGYRWRKYGQK VKNS
Sbjct: 152 EEKQDRQPTVKTDKQLKTKKRSPKKKGVEPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNS 211

Query: 128 PYPRSYYRCTSVACNVKKRVERCLKDPIIVITTYEGQHTHSTPVMARSTFFPLPISTSLY 187
           P+PRSYYRCTSVAC VKKRVERC +DP IV+TTYEGQHTH +P+MAR TFFP PIS +LY
Sbjct: 212 PHPRSYYRCTSVACQVKKRVERCFQDPSIVVTTYEGQHTHPSPIMARPTFFPPPISVTLY 271

Query: 188 EGSTSFTTTTPHLFHNQNVHNLNFARH 201
           +            +  QN HN N   H
Sbjct: 272 DD-----------YLIQNSHNSNVMSH 287

BLAST of CmaCh08G004800 vs. NCBI nr
Match: gi|525507288|ref|NP_001267684.1| (probable WRKY transcription factor 68-like [Cucumis sativus])

HSP 1 Score: 212.6 bits (540), Expect = 7.6e-52
Identity = 124/208 (59.62%), Postives = 147/208 (70.67%), Query Frame = 1

Query: 8   SQFDMFSTSSSPIQNPVI-DPENLEILNAWLTS----------SSQLINDELS-ELNLE- 67
           SQFD+F TSS  + + +I +PENLEI N W T+          SS+++N EL+ E NLE 
Sbjct: 39  SQFDVFETSSPSLSSSLISNPENLEIWNQWPTTPNYSSSISSTSSEIVNGELTTEPNLEG 98

Query: 68  -EQKP-RQKTVKSNPLSKTKKTNQKKKEQEQRYMFMTKSKVDHLEDGYRWRKYGQKVVKN 127
            EQK  +Q TVK++   KTKK + KKK  E R+ FMTKS+VDHLEDGYRWRKYGQK VKN
Sbjct: 99  GEQKQDQQPTVKADKQLKTKKRSPKKKGAEPRFAFMTKSEVDHLEDGYRWRKYGQKAVKN 158

Query: 128 SPYPRSYYRCTSVACNVKKRVERCLKDPIIVITTYEGQHTHSTPVMARSTFFPLPISTSL 187
           SP+PRSYYRCTSVACNVKKRVERCL+DP IV+TTYEGQHTH +P+MARSTFFP PIS +L
Sbjct: 159 SPHPRSYYRCTSVACNVKKRVERCLQDPSIVVTTYEGQHTHPSPIMARSTFFPPPISATL 218

Query: 188 YEGSTSFTTTTPHLFHNQNVHNLNFARH 201
           Y             +  QN HN N   H
Sbjct: 219 YND-----------YSIQNSHNSNVMSH 235

BLAST of CmaCh08G004800 vs. NCBI nr
Match: gi|659077791|ref|XP_008439387.1| (PREDICTED: probable WRKY transcription factor 48 [Cucumis melo])

HSP 1 Score: 164.9 bits (416), Expect = 1.8e-37
Identity = 98/204 (48.04%), Postives = 128/204 (62.75%), Query Frame = 1

Query: 8   SQFDMFSTSSSPIQNPVID-PENLEILNAWLTSSSQLINDELSELNLEEQKPRQKTVKSN 67
           S FD+ ST++ P+ +P    PE+ E++NA  T +S  ++   +E    E+     T    
Sbjct: 71  SFFDLLSTAAPPLSSPASTVPESSEVVNAPTTPNSSSVSSSSNEAAAIEEVNNSTTTHDK 130

Query: 68  PLS----KTKKTNQKKKEQEQRYMFMTKSKVDHLEDGYRWRKYGQKVVKNSPYPRSYYRC 127
           P S    +TKK NQKK ++E R+ FMTKS +DHL+DGYRWRKYGQK VKNSPYPRSYYRC
Sbjct: 131 PSSSKVVETKKKNQKK-QREPRFAFMTKSDIDHLDDGYRWRKYGQKAVKNSPYPRSYYRC 190

Query: 128 TSVACNVKKRVERCLKDPIIVITTYEGQHTHSTPVMARSTFFPLPISTSLYEGSTSFTTT 187
           T+  C VKKRVER   D  IV+TTYEGQHTH +P+M R +   LP ST+        TTT
Sbjct: 191 TTAGCGVKKRVERSSGDHTIVVTTYEGQHTHQSPIMPRGSLRVLPESTNNSLTVDHDTTT 250

Query: 188 TPHLF-HNQNVHNLNFARHPNDFV 206
           T  LF HN +     ++  P+ F+
Sbjct: 251 TGLLFQHNTSSQPFMYSSPPSPFL 273

BLAST of CmaCh08G004800 vs. NCBI nr
Match: gi|823187776|ref|XP_012490293.1| (PREDICTED: probable WRKY transcription factor 23 [Gossypium raimondii])

HSP 1 Score: 161.8 bits (408), Expect = 1.5e-36
Identity = 92/194 (47.42%), Postives = 128/194 (65.98%), Query Frame = 1

Query: 38  TSSSQLINDELSELNLEEQKPRQKTVKSNPLSKTKKTNQKKKEQEQRYMFMTKSKVDHLE 97
           ++SS+ ++DE ++L+ +E+  R    K+    K KKTNQK+ ++E R+ FMTKS+VDHLE
Sbjct: 104 SASSEAVHDEPAKLDDQEEDQR----KTKKQLKPKKTNQKR-QREPRFAFMTKSEVDHLE 163

Query: 98  DGYRWRKYGQKVVKNSPYPRSYYRCTSVACNVKKRVERCLKDPIIVITTYEGQHTHSTPV 157
           DGYRWRKYGQK VKNSP+PRSYYRCT+++CNVKKRVERC  DP IV+TTYEGQHTH +PV
Sbjct: 164 DGYRWRKYGQKAVKNSPFPRSYYRCTTISCNVKKRVERCFSDPSIVVTTYEGQHTHPSPV 223

Query: 158 MARSTFFPLPISTSLYEGSTSFT-TTTPHLFHNQNVHNLNFARHPNDFVAPIKF--HGSY 217
           M R       +++++   S   +  TTP  +H       +F +   D ++P+ F  +GS 
Sbjct: 224 MPRPNLVGSHLNSAISAASFGMSMQTTPSHYHQ------HFQQPFTDNLSPLNFGHNGSL 283

Query: 218 RFRWLKANANAIPG 229
              +L       PG
Sbjct: 284 NATFLHQKRFCTPG 286

BLAST of CmaCh08G004800 vs. NCBI nr
Match: gi|225437606|ref|XP_002277882.1| (PREDICTED: probable WRKY transcription factor 23 [Vitis vinifera])

HSP 1 Score: 161.0 bits (406), Expect = 2.6e-36
Identity = 93/197 (47.21%), Postives = 126/197 (63.96%), Query Frame = 1

Query: 16  SSSPIQNPVIDPENLEILNAWLTSSSQLINDELSELNLEEQKPRQKTVKSNPLSKTKKTN 75
           +SS + NP   P +        +S S   + E     +EE++  ++  K+    K KKT 
Sbjct: 76  ASSDVVNPPATPNS--------SSVSSASSHEQGSKAVEEEEEDEEEKKTKKELKPKKTT 135

Query: 76  QKKKEQEQRYMFMTKSKVDHLEDGYRWRKYGQKVVKNSPYPRSYYRCTSVACNVKKRVER 135
            +K+++E R+ FMTKS+VDHLEDGYRWRKYGQK VKNSP+PRSYYRCTS +CNVKKRVER
Sbjct: 136 SQKRQREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSASCNVKKRVER 195

Query: 136 CLKDPIIVITTYEGQHTHSTPVMARSTFFPLPISTSLYEGSTSFTTTTPHLFHNQNVHNL 195
           C KDP IV+TTYEGQHTH +P+M R+   P  I+T+ + G    TT   HL    ++H+ 
Sbjct: 196 CFKDPAIVVTTYEGQHTHPSPIMPRAN--PSSIATT-FAGPRLQTTPFQHL----HLHHH 255

Query: 196 NFARHPNDFVAPIKFHG 213
              +HP+    P+  +G
Sbjct: 256 QQQQHPSFNDVPLLNYG 257

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
WRK23_ARATH6.1e-3351.90Probable WRKY transcription factor 23 OS=Arabidopsis thaliana GN=WRKY23 PE=2 SV=... [more]
WRK71_ARATH5.7e-3151.53Probable WRKY transcription factor 71 OS=Arabidopsis thaliana GN=WRKY71 PE=2 SV=... [more]
WRKY8_ARATH9.7e-3154.29Probable WRKY transcription factor 8 OS=Arabidopsis thaliana GN=WRKY8 PE=1 SV=1[more]
WRK28_ARATH1.7e-3050.00Probable WRKY transcription factor 28 OS=Arabidopsis thaliana GN=WRKY28 PE=2 SV=... [more]
WRK48_ARATH3.7e-3041.12Probable WRKY transcription factor 48 OS=Arabidopsis thaliana GN=WRKY48 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
E7CEY6_CUCSA5.3e-5259.62Uncharacterized protein OS=Cucumis sativus GN=WRKY52 PE=2 SV=1[more]
A0A0D2P7Q0_GOSRA1.1e-3647.42Uncharacterized protein OS=Gossypium raimondii GN=B456_007G121200 PE=4 SV=1[more]
D7U2K4_VITVI1.8e-3647.21Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0005g01710 PE=4 SV=... [more]
K4EQ50_PANQU1.2e-3549.48WRKY28 OS=Panax quinquefolius PE=2 SV=1[more]
A0A140H8K6_MANES1.6e-3555.36WRKY transcription factor 2 OS=Manihot esculenta PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT2G47260.13.4e-3451.90 WRKY DNA-binding protein 23[more]
AT1G29860.13.2e-3251.53 WRKY DNA-binding protein 71[more]
AT5G46350.15.5e-3254.29 WRKY DNA-binding protein 8[more]
AT4G18170.19.3e-3250.00 WRKY DNA-binding protein 28[more]
AT5G49520.12.1e-3141.12 WRKY DNA-binding protein 48[more]
Match NameE-valueIdentityDescription
gi|659101173|ref|XP_008451465.1|3.4e-5257.97PREDICTED: probable WRKY transcription factor 23 [Cucumis melo][more]
gi|525507288|ref|NP_001267684.1|7.6e-5259.62probable WRKY transcription factor 68-like [Cucumis sativus][more]
gi|659077791|ref|XP_008439387.1|1.8e-3748.04PREDICTED: probable WRKY transcription factor 48 [Cucumis melo][more]
gi|823187776|ref|XP_012490293.1|1.5e-3647.42PREDICTED: probable WRKY transcription factor 23 [Gossypium raimondii][more]
gi|225437606|ref|XP_002277882.1|2.6e-3647.21PREDICTED: probable WRKY transcription factor 23 [Vitis vinifera][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR003657WRKY_dom
Vocabulary: Molecular Function
TermDefinition
GO:0003700transcription factor activity, sequence-specific DNA binding
GO:0043565sequence-specific DNA binding
Vocabulary: Biological Process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005667 transcription factor complex
molecular_function GO:0043565 sequence-specific DNA binding
molecular_function GO:0003700 transcription factor activity, sequence-specific DNA binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh08G004800.1CmaCh08G004800.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainGENE3DG3DSA:2.20.25.80coord: 81..156
score: 9.6
IPR003657WRKY domainPFAMPF03106WRKYcoord: 97..153
score: 2.3
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 96..155
score: 2.3
IPR003657WRKY domainPROFILEPS50811WRKYcoord: 91..156
score: 30
IPR003657WRKY domainunknownSSF118290WRKY DNA-binding domaincoord: 88..156
score: 6.67
NoneNo IPR availablePANTHERPTHR31221FAMILY NOT NAMEDcoord: 38..204
score: 1.5
NoneNo IPR availablePANTHERPTHR31221:SF13WRKY TRANSCRIPTION FACTOR 48-RELATEDcoord: 38..204
score: 1.5

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh08G004800CmaCh14G009400Cucurbita maxima (Rimu)cmacmaB293
CmaCh08G004800CmaCh17G010210Cucurbita maxima (Rimu)cmacmaB382
CmaCh08G004800CmaCh01G002790Cucurbita maxima (Rimu)cmacmaB440