CmaCh06G012110 (gene) Cucurbita maxima (Rimu)

NameCmaCh06G012110
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionE3 ubiquitin-protein ligase
LocationCma_Chr06 : 8078042 .. 8079519 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTTTTTGCAGATCAAAACCTTCTGCTTCGCTTCATTCATCAATGGATTCCGATTTTCCTCCGCTTTTCGGATGCCCGATTTCCATGGAGCTCATGGAGGATCCCGTCACCATCTCCACCGGCGTCACCTACGAGAGAAAAAGCATCGAAAAATGGGCTCATGGAGGATCCCGTCACCATCTCCACCGGCGTCACCTACGAGAGAAAAAGCATCGAAAAATGGCTCTTCACTTACAACAACAAAACCTGTCCGGCGACGATGCAGACCCTCGCCGCTGGCGACTTCGTCATCACTCCTAATCTCAATCTCAAACGCCTCATTCTCGCTTGGAAGATCACTGATTCTTCTGCGTCGCCGGCGACGATCGAGGAAGTTCGATCGCTGCTTGCGACTGTTGAGTCTTCTCCATTCAAGGTGAGTTCGTTGAGGAAATTGAGATCCATGATTGAAACGAGCGATGAGATGAAATCGGGATTTGTTCGATTGAATGGGATTGGAATTGTTGTGAATGTTGTCGTTCAAATTCTCATTGATTGTTCTGATTTTTGGACGTTTGAAGCTTGTGAAGAGGCTGTGGGAGTGTTGTCTCAGCTTCCGATTTCGAACGAAGACAAACTGTTCGACGAAATGTCTAAGAGAGAAGAATTGATGAAATCCATCGCTATTGTTCTTCAAAGGGGAAGTGCTGAAGGCCGTTTTTACGCTGTGAAAATCCTAAGAAATATTGCTAAAAACACCAATTTCAATTGGGGCTCTGTTTTGGAAGAACAGGGGATCGATTTCTTCAAGCCATTGTTGGAGCTTGTATCAGACGAATTCTCCACAAAGGCAAGCTCGTGCGCATTGGATGTAATGATGGAAATTTTAGCATTGTCAAAGAGAATTCTCCACAAAGGCAAGCTCGTGCGCATTGGATGTAATGATGGAAATTTTAGCATTGTCAAAGAGTAGTCGAGTGAAGGCGATCGAGGCCGGTGCTGTGTGCATCCTCATCGAGCTCTTACCGGAGTCAGGTCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCAGAGTTTGTGTGAATGTGCAGAGGGGATCAGGTCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCAGAGTTTGTGTGAATGTGCAGAGGGGAGATTGGCTTTGGTGGAACATGGGATGGGAATTGCAGCAGTGACTAAGAAGATTTTGAATGTTTCAAACGTAGCAACAAAAATGGGAGTGAAGATTTTGCTTTGGATTAGTAGTTTTCACCCCAATGAAAGGGTTGTGGAAGAGATGATGGCTTGTGGGGTAGTGAAGAAGCTGATGATGATATTGCACATGGACGGCGGCGGCGGTGGCCGGAGTTCGACGAAGGAGAAGGCGATCAAGATTTTGAAGATGCATGGGAGCTCATGGAGGCGTAGCCCTTGTTTTCCAAGTGAGTTGAAGAATTATTTGATGTTTGTGAATATTGAATGA

mRNA sequence

ATGAAGCTTTTTGCAGATCAAAACCTTCTGCTTCGCTTCATTCATCAATGGATTCCGATTTTCCTCCGCTTTTCGGATGCCCGATTTCCATGGAGCTCATGGAGGATCCCGTCACCATCTCCACCGGCGTCACCTACGAGAGAAAAAGCATCGAAAAATGGGCTCATGGAGGATCCCGTCACCATCTCCACCGGCGTCACCTACGAGAGAAAAAGCATCGAAAAATGGCTCTTCACTTACAACAACAAAACCTGTCCGGCGACGATGCAGACCCTCGCCGCTGGCGACTTCGTCATCACTCCTAATCTCAATCTCAAACGCCTCATTCTCGCTTGGAAGATCACTGATTCTTCTGCGTCGCCGGCGACGATCGAGGAAGTTCGATCGCTGCTTGCGACTGTTGAGTCTTCTCCATTCAAGGTGAGTTCGTTGAGGAAATTGAGATCCATGATTGAAACGAGCGATGAGATGAAATCGGGATTTGTTCGATTGAATGGGATTGGAATTGTTGTGAATGTTGTCGTTCAAATTCTCATTGATTGTTCTGATTTTTGGACGTTTGAAGCTTGTGAAGAGGCTGTGGGAGTGTTGTCTCAGCTTCCGATTTCGAACGAAGACAAACTGTTCGACGAAATGTCTAAGAGAGAAGAATTGATGAAATCCATCGCTATTGTTCTTCAAAGGGGAAGTGCTGAAGGCCGTTTTTACGCTGTGAAAATCCTAAGAAATATTGCTAAAAACACCAATTTCAATTGGGGCTCTGTTTTGGAAGAACAGGGGATCGATTTCTTCAAGCCATTGTTGGAGCTTGTATCAGACGAATTCTCCACAAAGGCAAGCTCGTGCGCATTGGATGTAATGATGGAAATTTTAGCATTGTCAAAGAGAATTCTCCACAAAGGCAAGCTCGTGCGCATTGGATGTAATGATGGAAATTTTAGCATTTCGAGTGAAGGCGATCGAGGCCGGTGCTGTGTGCATCCTCATCGAGCTCTTACCGGAGTCAGGTCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCAGAGTTTAGGGGATCAGGTCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCAGAGTTTGTGTGAATGTGCAGAGGGGAGATTGGCTTTGGTGGAACATGGGATGGGAATTGCAGCAGTGACTAAGAAGATTTTGAATGTTTCAAACGTAGCAACAAAAATGGGAGTGAAGATTTTGCTTTGGATTAGTAGTTTTCACCCCAATGAAAGGGTTGTGGAAGAGATGATGGCTTGTGGGGTAGTGAAGAAGCTGATGATGATATTGCACATGGACGGCGGCGGCGGTGGCCGGAGTTCGACGAAGGAGAAGGCGATCAAGATTTTGAAGATGCATGGGAGCTCATGGAGGCGTAGCCCTTGTTTTCCAAGTGAGTTGAAGAATTATTTGATGTTTGTGAATATTGAATGA

Coding sequence (CDS)

ATGAAGCTTTTTGCAGATCAAAACCTTCTGCTTCGCTTCATTCATCAATGGATTCCGATTTTCCTCCGCTTTTCGGATGCCCGATTTCCATGGAGCTCATGGAGGATCCCGTCACCATCTCCACCGGCGTCACCTACGAGAGAAAAAGCATCGAAAAATGGGCTCATGGAGGATCCCGTCACCATCTCCACCGGCGTCACCTACGAGAGAAAAAGCATCGAAAAATGGCTCTTCACTTACAACAACAAAACCTGTCCGGCGACGATGCAGACCCTCGCCGCTGGCGACTTCGTCATCACTCCTAATCTCAATCTCAAACGCCTCATTCTCGCTTGGAAGATCACTGATTCTTCTGCGTCGCCGGCGACGATCGAGGAAGTTCGATCGCTGCTTGCGACTGTTGAGTCTTCTCCATTCAAGGTGAGTTCGTTGAGGAAATTGAGATCCATGATTGAAACGAGCGATGAGATGAAATCGGGATTTGTTCGATTGAATGGGATTGGAATTGTTGTGAATGTTGTCGTTCAAATTCTCATTGATTGTTCTGATTTTTGGACGTTTGAAGCTTGTGAAGAGGCTGTGGGAGTGTTGTCTCAGCTTCCGATTTCGAACGAAGACAAACTGTTCGACGAAATGTCTAAGAGAGAAGAATTGATGAAATCCATCGCTATTGTTCTTCAAAGGGGAAGTGCTGAAGGCCGTTTTTACGCTGTGAAAATCCTAAGAAATATTGCTAAAAACACCAATTTCAATTGGGGCTCTGTTTTGGAAGAACAGGGGATCGATTTCTTCAAGCCATTGTTGGAGCTTGTATCAGACGAATTCTCCACAAAGGCAAGCTCGTGCGCATTGGATGTAATGATGGAAATTTTAGCATTGTCAAAGAGAATTCTCCACAAAGGCAAGCTCGTGCGCATTGGATGTAATGATGGAAATTTTAGCATTTCGAGTGAAGGCGATCGAGGCCGGTGCTGTGTGCATCCTCATCGAGCTCTTACCGGAGTCAGGTCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCAGAGTTTAGGGGATCAGGTCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCAGAGTTTGTGTGAATGTGCAGAGGGGAGATTGGCTTTGGTGGAACATGGGATGGGAATTGCAGCAGTGACTAAGAAGATTTTGAATGTTTCAAACGTAGCAACAAAAATGGGAGTGAAGATTTTGCTTTGGATTAGTAGTTTTCACCCCAATGAAAGGGTTGTGGAAGAGATGATGGCTTGTGGGGTAGTGAAGAAGCTGATGATGATATTGCACATGGACGGCGGCGGCGGTGGCCGGAGTTCGACGAAGGAGAAGGCGATCAAGATTTTGAAGATGCATGGGAGCTCATGGAGGCGTAGCCCTTGTTTTCCAAGTGAGTTGAAGAATTATTTGATGTTTGTGAATATTGAATGA

Protein sequence

MKLFADQNLLLRFIHQWIPIFLRFSDARFPWSSWRIPSPSPPASPTREKASKNGLMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAADSAEFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE
BLAST of CmaCh06G012110 vs. Swiss-Prot
Match: PUB23_ARATH (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana GN=PUB23 PE=1 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 4.7e-37
Identity = 134/434 (30.88%), Postives = 207/434 (47.70%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           +M+DPV +STG+TY+R SIEKWLF     +CP T Q +   D  +TPN  L+RLI +W  
Sbjct: 24  IMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD--LTPNHTLRRLIQSWCT 83

Query: 115 TDSSAS---------PATIEEVRSLLATVESS-PFKVSSLRKLRSMIETSDEMKSGFVRL 174
            ++S           P    E+  L+    SS   +V  L++LR ++  +   K      
Sbjct: 84  LNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIVSENATNKRCLEAA 143

Query: 175 NGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREE--LMKSI 234
                + N+V       +D       +EA+ +L  L  S E  L + ++ +++  ++KS+
Sbjct: 144 GVPEFLANIV------SNDSENGSLTDEALNLLYHLETS-ETVLKNLLNNKKDNNIVKSL 203

Query: 235 AIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSC 294
             ++QRG  E R YA  +L+NI +        V +       KP       E  T+    
Sbjct: 204 TKIMQRGMYESRVYATLLLKNILE--------VADPMQSMTLKP-------EVFTEVVQI 263

Query: 295 ALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRAL-TGVRS--IE 354
             D + +    +K  +H   LV I C  G               + H+A+  GV S  IE
Sbjct: 264 LDDRISQ--KATKAAMHI--LVNI-CPWGR--------------NRHKAVEAGVISVIIE 323

Query: 355 MRANAADSAEFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVA 414
           +  + + ++E RG      E  + +L  LC+CAEGR   + HG  IA V KKIL VS  A
Sbjct: 324 LLMDESFTSERRGP-----EMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTA 383

Query: 415 TKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMH 474
           +   V++LL +  F     ++ EM+  GVV KL ++L +  GG     TKEKA ++LK+H
Sbjct: 384 SDRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCGG----KTKEKAKELLKLH 405

BLAST of CmaCh06G012110 vs. Swiss-Prot
Match: PUB22_ARATH (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana GN=PUB22 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.3e-31
Identity = 124/458 (27.07%), Postives = 203/458 (44.32%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           +M+DPV +STG+TY+R+SIEKWLF+    +CP T Q +   D  +TPN  L+RLI +W  
Sbjct: 19  IMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD--LTPNHTLRRLIQSWCT 78

Query: 115 TDSS---------ASPATIEEVRSLLATVESSPF-KVSSLRKLRSMIETSDEMKSGFVRL 174
            ++S           P    E+  L+    SS   +V  L++LR ++  +   K      
Sbjct: 79  LNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIVSENTTNKRCLEAA 138

Query: 175 NGIGIVVNVVVQIL-------IDCSDFWTFEAC----------------EEAVGVLSQLP 234
                + N+V   +          S     + C                +EA+ VL  L 
Sbjct: 139 EVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRSLMDEALSVLYHLD 198

Query: 235 ISNED-KLFDEMSKREELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQG 294
            S    K      K   L+K++  ++QRG  E R YA  +L+ + +  +     +LE   
Sbjct: 199 TSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEVADPMQIILLER-- 258

Query: 295 IDFFKPLLELVSDEFSTKASSCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGD 354
            + F  +++++ D+ S KA+  A+ +++                 I C  G         
Sbjct: 259 -ELFGEVIQILHDQISHKATRSAMQILV-----------------ITCPWGR-------- 318

Query: 355 RGRCCVHPHRALTG---VRSIEMRANAADSAEFRGS--GRSKCERMLQILQSLCECAEGR 414
                 + H+A+ G      IE+  +   S+E R S       + + Q  +        R
Sbjct: 319 ------NRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEG-------R 378

Query: 415 LALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMI 474
              + HG  IA V+KKIL VS + ++  V++LL +  F     +++EM+  GVV KL ++
Sbjct: 379 AEFLNHGAAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLV 429

BLAST of CmaCh06G012110 vs. Swiss-Prot
Match: PUB24_ARATH (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana GN=PUB24 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.1e-28
Identity = 120/445 (26.97%), Postives = 209/445 (46.97%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAW-- 114
           +M+DPVT  +G+TY+R++I KWL      +CP T Q L   D  +TPN  L+RLI  W  
Sbjct: 22  IMKDPVTTVSGITYDRQNIVKWLEKV--PSCPVTKQPLPL-DSDLTPNHMLRRLIQHWCV 81

Query: 115 --------KITDSSASPA---TIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGF 174
                   +I+     P     +EE+++L    + +  +  +L+KL  +    +  +   
Sbjct: 82  ENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLEVLAMDGNNRRLMC 141

Query: 175 VRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPI-SNEDKLFDEMSKREELMK 234
                  +++ VV     D       +  +E++ +L  + I SN+ K    + + + +M+
Sbjct: 142 ECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPSNDAKTI--LMENDRVME 201

Query: 235 SIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKAS 294
           S+  VL +     + Y + +LRN+ + T+ +   ++E    + FK ++  + D  ++  +
Sbjct: 202 SLTWVLHQEDFLSKAYTIVLLRNLTEYTSSH---IVERLNPEIFKGIIGFLKDVVNS-VN 261

Query: 295 SCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEM 354
             +  V   + + S+  L K +  ++   D +  I          +    + +  RS+ +
Sbjct: 262 RTSPTVRETVQSSSRPSLGKTEPSKL---DHSLVIKQAVTAALMILLETSSWSRNRSLLV 321

Query: 355 RANAADSA---EFRGSGRSKC-ERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 414
              A       E   +G  +  E ML +L  LC CA GR  ++ H  GIA VTK++L VS
Sbjct: 322 DLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAVVTKRLLRVS 381

Query: 415 NVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKIL 474
             A    + IL  +S F P   VVEEM+  G V+KL  +L MD G     + KEKA +IL
Sbjct: 382 PAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCG----LNLKEKAKEIL 441

Query: 475 KMHGSSWRRSPCFPSELKNYLMFVN 482
           K H   W++ PC    L   L+ ++
Sbjct: 442 KDHFDEWKKFPCIDITLLTKLLSIS 450

BLAST of CmaCh06G012110 vs. Swiss-Prot
Match: PUB25_ARATH (U-box domain-containing protein 25 OS=Arabidopsis thaliana GN=PUB25 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 8.6e-23
Identity = 117/428 (27.34%), Postives = 187/428 (43.69%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           LM+DPVT+ TG TY+R SIE W+   NN TCP T   L+  DF + PN  L+RLI  W +
Sbjct: 26  LMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS--DFTLIPNHTLRRLIQEWCV 85

Query: 115 TDSS---------ASPATIEEVRSLLA-------TVESSPFKVSSLRKLRSMIETSDEMK 174
            + S           PA    VR+LL+       T  S   + ++LR+LR     SD+ +
Sbjct: 86  ANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRRLRGFARDSDKNR 145

Query: 175 SGFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 234
                 N   I++ ++       S+  + E   E++ +L  LPI+ E   F  +S     
Sbjct: 146 VLIAAHNATEILIKILF------SETTSSELVSESLALLVMLPIT-EPNQFVSISSDPGR 205

Query: 235 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFN--WGSVLEEQGIDFFKPLLELVSDEFS 294
           ++ +  +L   S E R  A  ++  ++  T      GS+   + +  F+ +L+L+ +  S
Sbjct: 206 VEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESV--FEGVLDLLRNPIS 265

Query: 295 TKASSCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALT-GV 354
           ++                       + ++IG        S +  R       H A+T G 
Sbjct: 266 SR-----------------------RALKIGIKTLFALCSVKSTR-------HIAITAGA 325

Query: 355 RSIEMRANAADSAEFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNV 414
             I +   AAD            ER L  ++ LC   EG  A  EH + +  + K IL V
Sbjct: 326 PEILIDRLAADFDRCD------TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRV 385

Query: 415 SNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKI 464
           S+ AT+     LL + +    ER  EE    GVV +L++++  +         K+KA K+
Sbjct: 386 SDRATEYAAGALLALCT--AEERWREEAAGAGVVVQLLLMVQSE----CTERAKKKAQKL 400

BLAST of CmaCh06G012110 vs. Swiss-Prot
Match: PUB21_ARATH (U-box domain-containing protein 21 OS=Arabidopsis thaliana GN=PUB21 PE=2 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 4.4e-19
Identity = 116/441 (26.30%), Postives = 192/441 (43.54%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           LM+DPV ISTG+TY+R SIE W+    NKTCP T   L   D +  PN  ++++I  W +
Sbjct: 43  LMKDPVIISTGITYDRVSIETWI-NSGNKTCPVTNTVLTTFDQI--PNHTIRKMIQGWCV 102

Query: 115 TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFV--RLNGIG---- 174
              S     I   R  L   E   +++S  RKL S     D  K G +  ++  +G    
Sbjct: 103 EKGSPLIQRIPTPRVPLMPCE--VYEIS--RKLSSATRRGDYEKCGVIIEKIKKLGDESE 162

Query: 175 -----IVVNVVVQILIDCSDFWTFEACEEAVGVLSQL--------PISNE--DKLFDEMS 234
                +  N V  +L DC D   F   E+   +L+++        PI  E   KL    S
Sbjct: 163 KNRKCVNENSVGWVLCDCFD--KFSGDEKLTFMLNEILSLLTWMFPIGLEGISKLASATS 222

Query: 235 KREELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSD 294
                 + +A +L+      R  A  I++ I         S   E G+   + L++L+ D
Sbjct: 223 -----FRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGV--AEALVKLIRD 282

Query: 295 EFSTKASSCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALT 354
             S+ ++  +L      +A+ + +L K ++       G  SI                  
Sbjct: 283 SVSSSSTKSSL------IAIYQMVLQKPEIASEFLEIGLVSI------------------ 342

Query: 355 GVRSIEMRANAADSAEFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKIL 414
              ++EM  +A +S           E+ L +L ++CE   GR  + ++ + +  + KKI 
Sbjct: 343 ---TVEMIVDAENSVC---------EKALAVLDAICETEHGREEVRKNALVMPLLVKKIA 402

Query: 415 NVSNVATK--MGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEK 472
            VS +AT+  M + + LW +    N   VE+ +  G  +K++++L +  G      TKEK
Sbjct: 403 KVSELATRSSMSMILKLWKTG---NTVAVEDAVRLGAFQKVLLVLQVGYG----EETKEK 424

BLAST of CmaCh06G012110 vs. TrEMBL
Match: A0A0A0L6J2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G127120 PE=4 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 1.1e-170
Identity = 328/435 (75.40%), Postives = 351/435 (80.69%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAG--DFVITPNLNLKRLILAW 114
           LMEDPVT+STGVTY+RKSIEKWLFTYN KTCPATMQTLAAG  DFVITPNLNLKRLILAW
Sbjct: 17  LMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAEDFVITPNLNLKRLILAW 76

Query: 115 KITDSSASPAT-IEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVV 174
           KI DS+ +PAT +EEV SLLATVESSPFKVSSLRKLRSM+E +D MKS F+RL+GIGIVV
Sbjct: 77  KINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMNDGMKSEFIRLSGIGIVV 136

Query: 175 NVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSA 234
           NVV+QILIDCSDF T + CEEA+GVL Q PISNEDKLFDEM KREELMKSIA+V+QRGSA
Sbjct: 137 NVVIQILIDCSDFSTLDTCEEALGVLCQFPISNEDKLFDEMYKREELMKSIAVVVQRGSA 196

Query: 235 EGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEIL 294
           EGRFYAVKILRNIAKNTN+NWGS++EEQGIDFFKPLLELVSDEF TKASSCALDVM    
Sbjct: 197 EGRFYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVM---- 256

Query: 295 ALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAADSAEFR 354
                                  I S   R R           V++IE  A         
Sbjct: 257 ---------------------IEILSSSKRSR-----------VKAIEAGAVWILIELLP 316

Query: 355 GSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWIS 414
            SGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS VATK+GVKILLWIS
Sbjct: 317 ESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWIS 376

Query: 415 SFHPNERVVEEMMACGVVKKLMMILHMDG---GGGGRSSTKEKAIKILKMHGSSWRRSPC 474
           SFHP ERVV+EMMACG VKKL+MILHMD    G GGRSSTKEK IKILKMHG+ WRRSPC
Sbjct: 377 SFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVIKILKMHGNKWRRSPC 415

Query: 475 FPSELKNYLMFVNIE 484
           FPSELKNYL FVN E
Sbjct: 437 FPSELKNYLKFVNDE 415

BLAST of CmaCh06G012110 vs. TrEMBL
Match: W9RFU0_9ROSA (E3 ubiquitin-protein ligase OS=Morus notabilis GN=L484_023862 PE=4 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 3.6e-108
Identity = 225/429 (52.45%), Postives = 299/429 (69.70%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAW-K 114
           LM+DPVTI TGVTY+RK+IEKW F+YN KTCPATMQT++  DF  TPN  LKRLIL+W K
Sbjct: 16  LMKDPVTIITGVTYDRKNIEKWFFSYNKKTCPATMQTIS--DFQPTPNHTLKRLILSWQK 75

Query: 115 ITDSSASPATIE----EVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGI 174
              SS S +T      E+  LLAT++SSPFKVSSL+KLRS+IE  DE K+  +  +GI +
Sbjct: 76  SKSSSVSSSTSSTKHGEILDLLATIDSSPFKVSSLKKLRSIIEIGDETKTDLIHSDGIEV 135

Query: 175 VVNVVVQILI-DCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQR 234
           ++ ++VQIL+ D SDF TF ACEE++ VL QLP+ +E K+FD +SKRE + KS+AI+LQR
Sbjct: 136 LIRILVQILVSDVSDFITFRACEESLAVLHQLPLCDEGKMFDLLSKRESI-KSMAIMLQR 195

Query: 235 GSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMM 294
            SAE R + + ILRN+AK T  +W  V+++QG+DFFK LLELVSDE   KASS AL+V++
Sbjct: 196 ASAETRLHTLSILRNMAK-TELDWSFVIQDQGVDFFKSLLELVSDEVCAKASSSALEVLI 255

Query: 295 EILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAADSA 354
           EIL+ SK    K +L  I             + G  CV           +E+  +A    
Sbjct: 256 EILSRSK----KSRLRAI-------------EAGAVCV----------LVELLPDA---- 315

Query: 355 EFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILL 414
               +   KCE++L +++ LCECAEGRLALVEHG+G+AAV+KK+L VS  ATK  VK+L 
Sbjct: 316 ----NRPQKCEKILLLIKLLCECAEGRLALVEHGIGMAAVSKKMLWVSGAATKAAVKVLW 375

Query: 415 WISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMHGSSWRRSPC 474
            + +FHP E+V+EEM+ CG VKKL+ +LHMD     RSSTK+K +KI K+HG+SW++ PC
Sbjct: 376 LVCNFHPTEKVLEEMLVCGTVKKLLALLHMD----ERSSTKDKVVKIFKLHGNSWKKYPC 401

Query: 475 FPSELKNYL 478
           FP + K+YL
Sbjct: 436 FPVDFKHYL 401

BLAST of CmaCh06G012110 vs. TrEMBL
Match: A0A059CEH8_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D01133 PE=4 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.3e-97
Identity = 217/439 (49.43%), Postives = 287/439 (65.38%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           +MEDPVTI+TG+TYERK+IEKW F Y  KTCPATMQ++ +  F ITPN  LKRLI+ W+ 
Sbjct: 15  VMEDPVTIATGMTYERKNIEKWFFAYKKKTCPATMQSVES--FGITPNHTLKRLIITWQS 74

Query: 115 TDSSASPATI--------EEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNG 174
                +P++         +E+ SLL  +ESSPFKV+SL+KLRSMIET DE K+ FV+  G
Sbjct: 75  KGGPGTPSSESRKSTERHDEIVSLLEALESSPFKVTSLKKLRSMIETDDEAKADFVQSGG 134

Query: 175 IGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVL 234
           IGI+V +  Q+L D SDF  F ACEEA+GVL QL +S E K  + +SK  E +K +  +L
Sbjct: 135 IGIIVRIASQVLEDGSDFSIFRACEEALGVLHQLSLSTEAKTLETLSK-PEAVKCVTAML 194

Query: 235 QRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDV 294
           QRGSAE R +A+ ILRN+A++   +W  +L++ GID FK LLELVSDE  T+ASS AL+V
Sbjct: 195 QRGSAEARSHAIIILRNMAQSET-HWTFILQDHGIDLFKSLLELVSDEICTRASSYALEV 254

Query: 295 MMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAAD 354
           ++E+L+ SK    K ++  I             + G  CV        +  +   +N + 
Sbjct: 255 LVEMLSASK----KSRVRAI-------------EAGAVCV--------LVELLPESNRSK 314

Query: 355 SAEFRGSGRSKCERMLQILQSLCEC----AEGRLALVEHGMGIAAVTKKILNVSNVATKM 414
           S +              +L+ LCEC         A+V+HGMGIAAVTKK+LNVSN ATK+
Sbjct: 315 SEKILM-----------LLKLLCECAEGRL----AMVDHGMGIAAVTKKMLNVSNAATKL 374

Query: 415 GVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMHGSS 474
            VKIL  I SFHP+ERV+EEM+  G VKKL+ +LH+D    GRSSTKEK IK+ KMH +S
Sbjct: 375 SVKILWLIGSFHPSERVLEEMLIYGSVKKLLALLHID----GRSSTKEKVIKMFKMHCNS 405

Query: 475 WRRSPCFPSELKNYLMFVN 482
           W+R PCFP+E+K+YL  VN
Sbjct: 435 WQRCPCFPNEMKDYLGMVN 405

BLAST of CmaCh06G012110 vs. TrEMBL
Match: A0A0A0KBP6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G079190 PE=4 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.1e-96
Identity = 217/442 (49.10%), Postives = 281/442 (63.57%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           LM+DPVTISTGV++ER +IEKW FTYN KTCPATMQT+   +F ITPN  LKRLIL+WK+
Sbjct: 34  LMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIV--NFDITPNYTLKRLILSWKM 93

Query: 115 TDSSAS------PATIEE----VRSLLATVESSPFKVSSLRKLRSMIETSDEMKSG---- 174
            D S S      P++  E    V  LL  +ESSPFK SSL+KLR ++   D    G    
Sbjct: 94  KDQSCSSTSSLLPSSSMEYHDVVADLLKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPV 153

Query: 175 FVRLNGIGIVVNVVVQILI-DCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELM 234
           FVR+NG  +++ +V Q+ I + SDF  FEACEEA+GVLSQ P S  +K F+ +S+ E + 
Sbjct: 154 FVRVNGFEVLIGIVTQVAICESSDFANFEACEEALGVLSQFPFSKTEKPFELLSEPEAI- 213

Query: 235 KSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKA 294
           KS+AIVLQRGS EGRFYA+++L+ I+K   ++W S+ + Q ID FK +LEL SDE   KA
Sbjct: 214 KSMAIVLQRGSIEGRFYAMEMLQQISKK-GYDWNSLTKHQIIDLFKSILELASDEIIKKA 273

Query: 295 SSCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIE 354
           S+        +       L    L  +        I     + R           + SIE
Sbjct: 274 SAIKTTDSSIVTHCRYGTLFSSALELL------IEIMESSKKSR-----------LLSIE 333

Query: 355 MRANAADSAEFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVA 414
             A          S RSKCE++L IL+ L ECAEGR A+ EH MGIA VTKK +++SN A
Sbjct: 334 AGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK-MSISNTA 393

Query: 415 TKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMH 474
           TK+G+KIL  I ++HP+ERV+EEMM CG VKKL+ IL+    G GRSSTKEKA K++K+H
Sbjct: 394 TKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILN---NGDGRSSTKEKAKKVMKLH 450

Query: 475 GSSWRRSPCFPSELKNYLMFVN 482
           GS WRR PCFP E ++YL  +N
Sbjct: 454 GSFWRRFPCFPYEYRDYLKLIN 450

BLAST of CmaCh06G012110 vs. TrEMBL
Match: A0A0D2S6G5_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_013G013600 PE=4 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 2.0e-95
Identity = 202/432 (46.76%), Postives = 278/432 (64.35%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           +M+DPVTI TGV+YERK+IEKW F YN KTCP TMQ + + D  +TPN  LKRLILAWK 
Sbjct: 18  IMKDPVTIVTGVSYERKNIEKWFFVYNKKTCPTTMQCVESFD--MTPNHTLKRLILAWKE 77

Query: 115 T------DSSASPATI---EEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLN 174
           T       SS+ P  +   +E+ SL   +ESSPFKVSS++K+R+++E  DE KS F+R  
Sbjct: 78  TLVAPSSTSSSMPQPLIKHDEMVSLFTALESSPFKVSSMKKIRAIVELGDETKSDFIRSG 137

Query: 175 GIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIV 234
           G+  VV +++    D SDF +F+ACEEA+G+L  LP+S +D+   ++  + + M+SIAI+
Sbjct: 138 GVEAVVGMLIN-QFDNSDFVSFQACEEALGILHLLPLSKQDEKSFQLISKPDPMRSIAII 197

Query: 235 LQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALD 294
           LQRGSAE RFYA+ I R IAK T FNW S++E+QGID FK LLELV D    KASS AL+
Sbjct: 198 LQRGSAEARFYAITIFRKIAK-TGFNWNSLIEDQGIDLFKSLLELVYDGICNKASSYALE 257

Query: 295 VMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAA 354
           +                          F I S   + R           +++IE  A   
Sbjct: 258 I-------------------------LFEILSSSKKSR-----------LKAIEAGAICI 317

Query: 355 DSAEFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVK 414
                  S RSK E+ML +++ LCE  EGR+A+V+H +GI  ++K +L VSN+ATK+GVK
Sbjct: 318 LIELLPDSNRSKSEKMLLLIKLLCESPEGRMAMVDHSLGIPVISKTLLQVSNLATKLGVK 377

Query: 415 ILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMHGSSWRR 474
           IL  + +FHP ERV+EEM+  G VKK++ +LH++G     SSTK+K +++LKMH +SW+R
Sbjct: 378 ILWQVCNFHPTERVLEEMLMHGAVKKMVTLLHLEGR---LSSTKKKVLEMLKMHCNSWKR 406

Query: 475 SPCFPSELKNYL 478
             CFP +LK+YL
Sbjct: 438 HQCFPCDLKDYL 406

BLAST of CmaCh06G012110 vs. TAIR10
Match: AT2G35930.1 (AT2G35930.1 plant U-box 23)

HSP 1 Score: 157.1 bits (396), Expect = 2.7e-38
Identity = 134/434 (30.88%), Postives = 207/434 (47.70%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           +M+DPV +STG+TY+R SIEKWLF     +CP T Q +   D  +TPN  L+RLI +W  
Sbjct: 24  IMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD--LTPNHTLRRLIQSWCT 83

Query: 115 TDSSAS---------PATIEEVRSLLATVESS-PFKVSSLRKLRSMIETSDEMKSGFVRL 174
            ++S           P    E+  L+    SS   +V  L++LR ++  +   K      
Sbjct: 84  LNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIVSENATNKRCLEAA 143

Query: 175 NGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREE--LMKSI 234
                + N+V       +D       +EA+ +L  L  S E  L + ++ +++  ++KS+
Sbjct: 144 GVPEFLANIV------SNDSENGSLTDEALNLLYHLETS-ETVLKNLLNNKKDNNIVKSL 203

Query: 235 AIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSC 294
             ++QRG  E R YA  +L+NI +        V +       KP       E  T+    
Sbjct: 204 TKIMQRGMYESRVYATLLLKNILE--------VADPMQSMTLKP-------EVFTEVVQI 263

Query: 295 ALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRAL-TGVRS--IE 354
             D + +    +K  +H   LV I C  G               + H+A+  GV S  IE
Sbjct: 264 LDDRISQ--KATKAAMHI--LVNI-CPWGR--------------NRHKAVEAGVISVIIE 323

Query: 355 MRANAADSAEFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVA 414
           +  + + ++E RG      E  + +L  LC+CAEGR   + HG  IA V KKIL VS  A
Sbjct: 324 LLMDESFTSERRGP-----EMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTA 383

Query: 415 TKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMH 474
           +   V++LL +  F     ++ EM+  GVV KL ++L +  GG     TKEKA ++LK+H
Sbjct: 384 SDRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCGG----KTKEKAKELLKLH 405

BLAST of CmaCh06G012110 vs. TAIR10
Match: AT3G52450.1 (AT3G52450.1 plant U-box 22)

HSP 1 Score: 139.0 bits (349), Expect = 7.5e-33
Identity = 124/458 (27.07%), Postives = 203/458 (44.32%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           +M+DPV +STG+TY+R+SIEKWLF+    +CP T Q +   D  +TPN  L+RLI +W  
Sbjct: 19  IMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD--LTPNHTLRRLIQSWCT 78

Query: 115 TDSS---------ASPATIEEVRSLLATVESSPF-KVSSLRKLRSMIETSDEMKSGFVRL 174
            ++S           P    E+  L+    SS   +V  L++LR ++  +   K      
Sbjct: 79  LNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIVSENTTNKRCLEAA 138

Query: 175 NGIGIVVNVVVQIL-------IDCSDFWTFEAC----------------EEAVGVLSQLP 234
                + N+V   +          S     + C                +EA+ VL  L 
Sbjct: 139 EVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRSLMDEALSVLYHLD 198

Query: 235 ISNED-KLFDEMSKREELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQG 294
            S    K      K   L+K++  ++QRG  E R YA  +L+ + +  +     +LE   
Sbjct: 199 TSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEVADPMQIILLER-- 258

Query: 295 IDFFKPLLELVSDEFSTKASSCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGD 354
            + F  +++++ D+ S KA+  A+ +++                 I C  G         
Sbjct: 259 -ELFGEVIQILHDQISHKATRSAMQILV-----------------ITCPWGR-------- 318

Query: 355 RGRCCVHPHRALTG---VRSIEMRANAADSAEFRGS--GRSKCERMLQILQSLCECAEGR 414
                 + H+A+ G      IE+  +   S+E R S       + + Q  +        R
Sbjct: 319 ------NRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEG-------R 378

Query: 415 LALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMI 474
              + HG  IA V+KKIL VS + ++  V++LL +  F     +++EM+  GVV KL ++
Sbjct: 379 AEFLNHGAAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLV 429

BLAST of CmaCh06G012110 vs. TAIR10
Match: AT3G11840.1 (AT3G11840.1 plant U-box 24)

HSP 1 Score: 129.4 bits (324), Expect = 5.9e-30
Identity = 120/445 (26.97%), Postives = 209/445 (46.97%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAW-- 114
           +M+DPVT  +G+TY+R++I KWL      +CP T Q L   D  +TPN  L+RLI  W  
Sbjct: 36  IMKDPVTTVSGITYDRQNIVKWLEKV--PSCPVTKQPLPL-DSDLTPNHMLRRLIQHWCV 95

Query: 115 --------KITDSSASPA---TIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGF 174
                   +I+     P     +EE+++L    + +  +  +L+KL  +    +  +   
Sbjct: 96  ENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLEVLAMDGNNRRLMC 155

Query: 175 VRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPI-SNEDKLFDEMSKREELMK 234
                  +++ VV     D       +  +E++ +L  + I SN+ K    + + + +M+
Sbjct: 156 ECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPSNDAKTI--LMENDRVME 215

Query: 235 SIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKAS 294
           S+  VL +     + Y + +LRN+ + T+ +   ++E    + FK ++  + D  ++  +
Sbjct: 216 SLTWVLHQEDFLSKAYTIVLLRNLTEYTSSH---IVERLNPEIFKGIIGFLKDVVNS-VN 275

Query: 295 SCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEM 354
             +  V   + + S+  L K +  ++   D +  I          +    + +  RS+ +
Sbjct: 276 RTSPTVRETVQSSSRPSLGKTEPSKL---DHSLVIKQAVTAALMILLETSSWSRNRSLLV 335

Query: 355 RANAADSA---EFRGSGRSKC-ERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 414
              A       E   +G  +  E ML +L  LC CA GR  ++ H  GIA VTK++L VS
Sbjct: 336 DLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAVVTKRLLRVS 395

Query: 415 NVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKIL 474
             A    + IL  +S F P   VVEEM+  G V+KL  +L MD G     + KEKA +IL
Sbjct: 396 PAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCG----LNLKEKAKEIL 455

Query: 475 KMHGSSWRRSPCFPSELKNYLMFVN 482
           K H   W++ PC    L   L+ ++
Sbjct: 456 KDHFDEWKKFPCIDITLLTKLLSIS 464

BLAST of CmaCh06G012110 vs. TAIR10
Match: AT3G19380.1 (AT3G19380.1 plant U-box 25)

HSP 1 Score: 109.8 bits (273), Expect = 4.9e-24
Identity = 117/428 (27.34%), Postives = 187/428 (43.69%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           LM+DPVT+ TG TY+R SIE W+   NN TCP T   L+  DF + PN  L+RLI  W +
Sbjct: 26  LMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS--DFTLIPNHTLRRLIQEWCV 85

Query: 115 TDSS---------ASPATIEEVRSLLA-------TVESSPFKVSSLRKLRSMIETSDEMK 174
            + S           PA    VR+LL+       T  S   + ++LR+LR     SD+ +
Sbjct: 86  ANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRRLRGFARDSDKNR 145

Query: 175 SGFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 234
                 N   I++ ++       S+  + E   E++ +L  LPI+ E   F  +S     
Sbjct: 146 VLIAAHNATEILIKILF------SETTSSELVSESLALLVMLPIT-EPNQFVSISSDPGR 205

Query: 235 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFN--WGSVLEEQGIDFFKPLLELVSDEFS 294
           ++ +  +L   S E R  A  ++  ++  T      GS+   + +  F+ +L+L+ +  S
Sbjct: 206 VEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESV--FEGVLDLLRNPIS 265

Query: 295 TKASSCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALT-GV 354
           ++                       + ++IG        S +  R       H A+T G 
Sbjct: 266 SR-----------------------RALKIGIKTLFALCSVKSTR-------HIAITAGA 325

Query: 355 RSIEMRANAADSAEFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNV 414
             I +   AAD            ER L  ++ LC   EG  A  EH + +  + K IL V
Sbjct: 326 PEILIDRLAADFDRCD------TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRV 385

Query: 415 SNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKI 464
           S+ AT+     LL + +    ER  EE    GVV +L++++  +         K+KA K+
Sbjct: 386 SDRATEYAAGALLALCT--AEERWREEAAGAGVVVQLLLMVQSE----CTERAKKKAQKL 400

BLAST of CmaCh06G012110 vs. TAIR10
Match: AT5G37490.1 (AT5G37490.1 ARM repeat superfamily protein)

HSP 1 Score: 97.4 bits (241), Expect = 2.5e-20
Identity = 116/441 (26.30%), Postives = 192/441 (43.54%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           LM+DPV ISTG+TY+R SIE W+    NKTCP T   L   D +  PN  ++++I  W +
Sbjct: 43  LMKDPVIISTGITYDRVSIETWI-NSGNKTCPVTNTVLTTFDQI--PNHTIRKMIQGWCV 102

Query: 115 TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFV--RLNGIG---- 174
              S     I   R  L   E   +++S  RKL S     D  K G +  ++  +G    
Sbjct: 103 EKGSPLIQRIPTPRVPLMPCE--VYEIS--RKLSSATRRGDYEKCGVIIEKIKKLGDESE 162

Query: 175 -----IVVNVVVQILIDCSDFWTFEACEEAVGVLSQL--------PISNE--DKLFDEMS 234
                +  N V  +L DC D   F   E+   +L+++        PI  E   KL    S
Sbjct: 163 KNRKCVNENSVGWVLCDCFD--KFSGDEKLTFMLNEILSLLTWMFPIGLEGISKLASATS 222

Query: 235 KREELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSD 294
                 + +A +L+      R  A  I++ I         S   E G+   + L++L+ D
Sbjct: 223 -----FRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGV--AEALVKLIRD 282

Query: 295 EFSTKASSCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALT 354
             S+ ++  +L      +A+ + +L K ++       G  SI                  
Sbjct: 283 SVSSSSTKSSL------IAIYQMVLQKPEIASEFLEIGLVSI------------------ 342

Query: 355 GVRSIEMRANAADSAEFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKIL 414
              ++EM  +A +S           E+ L +L ++CE   GR  + ++ + +  + KKI 
Sbjct: 343 ---TVEMIVDAENSVC---------EKALAVLDAICETEHGREEVRKNALVMPLLVKKIA 402

Query: 415 NVSNVATK--MGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEK 472
            VS +AT+  M + + LW +    N   VE+ +  G  +K++++L +  G      TKEK
Sbjct: 403 KVSELATRSSMSMILKLWKTG---NTVAVEDAVRLGAFQKVLLVLQVGYG----EETKEK 424

BLAST of CmaCh06G012110 vs. NCBI nr
Match: gi|449433143|ref|XP_004134357.1| (PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis sativus])

HSP 1 Score: 607.8 bits (1566), Expect = 1.6e-170
Identity = 328/435 (75.40%), Postives = 351/435 (80.69%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAG--DFVITPNLNLKRLILAW 114
           LMEDPVT+STGVTY+RKSIEKWLFTYN KTCPATMQTLAAG  DFVITPNLNLKRLILAW
Sbjct: 17  LMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAEDFVITPNLNLKRLILAW 76

Query: 115 KITDSSASPAT-IEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVV 174
           KI DS+ +PAT +EEV SLLATVESSPFKVSSLRKLRSM+E +D MKS F+RL+GIGIVV
Sbjct: 77  KINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMNDGMKSEFIRLSGIGIVV 136

Query: 175 NVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSA 234
           NVV+QILIDCSDF T + CEEA+GVL Q PISNEDKLFDEM KREELMKSIA+V+QRGSA
Sbjct: 137 NVVIQILIDCSDFSTLDTCEEALGVLCQFPISNEDKLFDEMYKREELMKSIAVVVQRGSA 196

Query: 235 EGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEIL 294
           EGRFYAVKILRNIAKNTN+NWGS++EEQGIDFFKPLLELVSDEF TKASSCALDVM    
Sbjct: 197 EGRFYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVM---- 256

Query: 295 ALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAADSAEFR 354
                                  I S   R R           V++IE  A         
Sbjct: 257 ---------------------IEILSSSKRSR-----------VKAIEAGAVWILIELLP 316

Query: 355 GSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWIS 414
            SGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS VATK+GVKILLWIS
Sbjct: 317 ESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWIS 376

Query: 415 SFHPNERVVEEMMACGVVKKLMMILHMDG---GGGGRSSTKEKAIKILKMHGSSWRRSPC 474
           SFHP ERVV+EMMACG VKKL+MILHMD    G GGRSSTKEK IKILKMHG+ WRRSPC
Sbjct: 377 SFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVIKILKMHGNKWRRSPC 415

Query: 475 FPSELKNYLMFVNIE 484
           FPSELKNYL FVN E
Sbjct: 437 FPSELKNYLKFVNDE 415

BLAST of CmaCh06G012110 vs. NCBI nr
Match: gi|659075605|ref|XP_008438233.1| (PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo])

HSP 1 Score: 604.4 bits (1557), Expect = 1.8e-169
Identity = 329/435 (75.63%), Postives = 349/435 (80.23%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAG--DFVITPNLNLKRLILAW 114
           LMEDPVT+STGVTY+RKSIEKWLFTYN KTCPATMQTLAAG  DFVITPNL LKRLILAW
Sbjct: 17  LMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADDFVITPNLTLKRLILAW 76

Query: 115 KITDSSASPAT-IEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVV 174
           KI DS+++PAT +EEV SLLATVESSPFKVSSLRKLRS+IE +D MKS FVRL+GIGIVV
Sbjct: 77  KINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMNDGMKSEFVRLSGIGIVV 136

Query: 175 NVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSA 234
           NVV+QILIDCSDF T E CEEA+GVL Q PISNEDKLFDEM KREELMKSI +VLQRGSA
Sbjct: 137 NVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKREELMKSITVVLQRGSA 196

Query: 235 EGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEIL 294
           EGRFYAVKILRNIAKNTN+NWGSV+EEQ IDFFKPLLELVSDEF TKASSCALDVM    
Sbjct: 197 EGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEFPTKASSCALDVM---- 256

Query: 295 ALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAADSAEFR 354
                                  I S   R R           V++IE  A         
Sbjct: 257 ---------------------IGILSSSKRSR-----------VKAIEAGAVWILIELLP 316

Query: 355 GSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWIS 414
            SGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS VATK+GVKILLWIS
Sbjct: 317 ESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWIS 376

Query: 415 SFHPNERVVEEMMACGVVKKLMMILHMDG---GGGGRSSTKEKAIKILKMHGSSWRRSPC 474
           SFHP ERVV+EMMACG VKKL+MILHMD    G GGRSSTKEK IKILKMHG+ WRRSPC
Sbjct: 377 SFHPRERVVDEMMACGAVKKLLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPC 415

Query: 475 FPSELKNYLMFVNIE 484
           FPSELKNYL FVN E
Sbjct: 437 FPSELKNYLKFVNDE 415

BLAST of CmaCh06G012110 vs. NCBI nr
Match: gi|703103411|ref|XP_010097721.1| (E3 ubiquitin-protein ligase [Morus notabilis])

HSP 1 Score: 400.2 bits (1027), Expect = 5.1e-108
Identity = 225/429 (52.45%), Postives = 299/429 (69.70%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAW-K 114
           LM+DPVTI TGVTY+RK+IEKW F+YN KTCPATMQT++  DF  TPN  LKRLIL+W K
Sbjct: 16  LMKDPVTIITGVTYDRKNIEKWFFSYNKKTCPATMQTIS--DFQPTPNHTLKRLILSWQK 75

Query: 115 ITDSSASPATIE----EVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGI 174
              SS S +T      E+  LLAT++SSPFKVSSL+KLRS+IE  DE K+  +  +GI +
Sbjct: 76  SKSSSVSSSTSSTKHGEILDLLATIDSSPFKVSSLKKLRSIIEIGDETKTDLIHSDGIEV 135

Query: 175 VVNVVVQILI-DCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQR 234
           ++ ++VQIL+ D SDF TF ACEE++ VL QLP+ +E K+FD +SKRE + KS+AI+LQR
Sbjct: 136 LIRILVQILVSDVSDFITFRACEESLAVLHQLPLCDEGKMFDLLSKRESI-KSMAIMLQR 195

Query: 235 GSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMM 294
            SAE R + + ILRN+AK T  +W  V+++QG+DFFK LLELVSDE   KASS AL+V++
Sbjct: 196 ASAETRLHTLSILRNMAK-TELDWSFVIQDQGVDFFKSLLELVSDEVCAKASSSALEVLI 255

Query: 295 EILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAADSA 354
           EIL+ SK    K +L  I             + G  CV           +E+  +A    
Sbjct: 256 EILSRSK----KSRLRAI-------------EAGAVCV----------LVELLPDA---- 315

Query: 355 EFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILL 414
               +   KCE++L +++ LCECAEGRLALVEHG+G+AAV+KK+L VS  ATK  VK+L 
Sbjct: 316 ----NRPQKCEKILLLIKLLCECAEGRLALVEHGIGMAAVSKKMLWVSGAATKAAVKVLW 375

Query: 415 WISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMHGSSWRRSPC 474
            + +FHP E+V+EEM+ CG VKKL+ +LHMD     RSSTK+K +KI K+HG+SW++ PC
Sbjct: 376 LVCNFHPTEKVLEEMLVCGTVKKLLALLHMD----ERSSTKDKVVKIFKLHGNSWKKYPC 401

Query: 475 FPSELKNYL 478
           FP + K+YL
Sbjct: 436 FPVDFKHYL 401

BLAST of CmaCh06G012110 vs. NCBI nr
Match: gi|720061726|ref|XP_010275264.1| (PREDICTED: E3 ubiquitin-protein ligase PUB22-like [Nelumbo nucifera])

HSP 1 Score: 397.5 bits (1020), Expect = 3.3e-107
Identity = 228/434 (52.53%), Postives = 296/434 (68.20%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           +M+DPVTISTGVTYERK+IE W F YN KTCPATMQ + + D  +TPNL LK LILAW+ 
Sbjct: 28  IMKDPVTISTGVTYERKNIETWFFKYNKKTCPATMQCIQSFD--LTPNLTLKSLILAWQD 87

Query: 115 TDS----SASPATIE--EVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSG-FVRLNGI 174
            +S    S+SP + +  E+ SLL T+ES+PFKVSSL+KLR++IE +D+   G FV+  G+
Sbjct: 88  RESQFRLSSSPISTKHTELVSLLTTMESTPFKVSSLKKLRAIIEFNDDAVMGDFVQSGGV 147

Query: 175 GIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQ 234
            ++  ++VQIL+D SDF  F ACEEA+GVL QLP+S+E  +  ++  + E MKS+AI+LQ
Sbjct: 148 DVLSRIIVQILVDSSDFMAFRACEEALGVLHQLPLSDESSI--QLLSKPECMKSMAIMLQ 207

Query: 235 RGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVM 294
           RGSAE R + V + R +AK   +NW  V+E+QG+D FK LLEL+SDE STKASS ALD+ 
Sbjct: 208 RGSAEARLHTVSMFRKMAK-VEYNWSWVVEDQGMDLFKSLLELLSDELSTKASSSALDI- 267

Query: 295 MEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAADS 354
                    IL+  K  R+   +           G  CV           IE+  +A   
Sbjct: 268 ------LIEILNASKKNRLKAIEA----------GAVCV----------LIELLPDA--- 327

Query: 355 AEFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKIL 414
                  RSKCE+ML I++ LCECAEGRLALVEH +GIAA+TKKIL VS++ TK GVKI 
Sbjct: 328 ------NRSKCEKMLVIIKLLCECAEGRLALVEHSLGIAAITKKILRVSDLVTKYGVKIF 387

Query: 415 LWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMHGSSWRRSP 474
             I +FHP E+VVEEM+  G VKKL+ +LHMD    GRSSTK+KA+KI+K+H +SWR+ P
Sbjct: 388 WLICNFHPTEKVVEEMLVYGSVKKLVALLHMD----GRSSTKDKALKIIKLHENSWRQYP 416

Query: 475 CFPSELKNYLMFVN 482
           CFPSELK YL  +N
Sbjct: 448 CFPSELKEYLKLLN 416

BLAST of CmaCh06G012110 vs. NCBI nr
Match: gi|1009110389|ref|XP_015895309.1| (PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Ziziphus jujuba])

HSP 1 Score: 382.9 bits (982), Expect = 8.4e-103
Identity = 222/454 (48.90%), Postives = 289/454 (63.66%), Query Frame = 1

Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           LM DPVTISTGV+YERK+IEKW FTYN +TCPATMQ ++  +  ITPN  LKRLIL+W+ 
Sbjct: 42  LMTDPVTISTGVSYERKNIEKWFFTYNKRTCPATMQPISDFNSQITPNHTLKRLILSWQN 101

Query: 115 TDS------------SASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFV 174
            +             S S    +E+ SLL T++SSPFKV SL+KLRS++E SD+ K+ FV
Sbjct: 102 KNKNKNHQINHHHQPSPSSNKHDEIVSLLNTIQSSPFKVPSLKKLRSIVEISDDTKNDFV 161

Query: 175 RLNGIGIVVNVVVQILI--DCSDFWTFEACEEAVGVLSQLPISNEDKL------------ 234
             +G+ +VV ++ QIL+    SDF TF ACEEA+GVL QLP+S+                
Sbjct: 162 SCSGVEVVVGILHQILVVDTSSDFITFRACEEALGVLHQLPLSSSSSSPPSSSSSSSSSS 221

Query: 235 -FDEMSKREELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLE-EQGIDFFKP 294
               +  + E M+ +AI+LQR SA+ R YAV ILR +AK T+++WG +++  QG+ FFK 
Sbjct: 222 SLSSLLSKPESMRCMAIMLQRASADTRLYAVTILRKLAK-TDYDWGFMIQGNQGVGFFKS 281

Query: 295 LLELVSDEFSTKASSCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCV 354
           LLE+VSDE STK SSCAL+V+         IL K K  R+                    
Sbjct: 282 LLEMVSDEISTKTSSCALEVL-------IEILSKSKKSRL-------------------- 341

Query: 355 HPHRALTGVRSIEMRANAADSAEFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIA 414
                    R+IE  A          S R +CERMLQ+++ LCECAEGRL LVEHGMGIA
Sbjct: 342 ---------RAIEAGAVCVLVEVLPDSNRPRCERMLQLIKLLCECAEGRLGLVEHGMGIA 401

Query: 415 AVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRS 474
           A++KK+L VSNVATK+ VKIL  I +FH  ERV++EM+ CG VKKL+ +LH+D    GRS
Sbjct: 402 AISKKMLQVSNVATKIVVKILWLICNFHATERVLDEMLICGAVKKLLALLHID----GRS 454

Query: 475 STKEKAIKILKMHGSSWRRSPCFPSELKNYLMFV 481
           STK+K +KI K+HG  W R PCFPS+LK++L FV
Sbjct: 462 STKDKVVKIFKLHGHYWSRFPCFPSDLKDFLGFV 454

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PUB23_ARATH4.7e-3730.88E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana GN=PUB23 PE=1 SV=1[more]
PUB22_ARATH1.3e-3127.07E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana GN=PUB22 PE=1 SV=1[more]
PUB24_ARATH1.1e-2826.97E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana GN=PUB24 PE=1 SV=1[more]
PUB25_ARATH8.6e-2327.34U-box domain-containing protein 25 OS=Arabidopsis thaliana GN=PUB25 PE=2 SV=1[more]
PUB21_ARATH4.4e-1926.30U-box domain-containing protein 21 OS=Arabidopsis thaliana GN=PUB21 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L6J2_CUCSA1.1e-17075.40Uncharacterized protein OS=Cucumis sativus GN=Csa_3G127120 PE=4 SV=1[more]
W9RFU0_9ROSA3.6e-10852.45E3 ubiquitin-protein ligase OS=Morus notabilis GN=L484_023862 PE=4 SV=1[more]
A0A059CEH8_EUCGR1.3e-9749.43Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D01133 PE=4 SV=1[more]
A0A0A0KBP6_CUCSA1.1e-9649.10Uncharacterized protein OS=Cucumis sativus GN=Csa_6G079190 PE=4 SV=1[more]
A0A0D2S6G5_GOSRA2.0e-9546.76Uncharacterized protein OS=Gossypium raimondii GN=B456_013G013600 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G35930.12.7e-3830.88 plant U-box 23[more]
AT3G52450.17.5e-3327.07 plant U-box 22[more]
AT3G11840.15.9e-3026.97 plant U-box 24[more]
AT3G19380.14.9e-2427.34 plant U-box 25[more]
AT5G37490.12.5e-2026.30 ARM repeat superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449433143|ref|XP_004134357.1|1.6e-17075.40PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis sativus][more]
gi|659075605|ref|XP_008438233.1|1.8e-16975.63PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo][more]
gi|703103411|ref|XP_010097721.1|5.1e-10852.45E3 ubiquitin-protein ligase [Morus notabilis][more]
gi|720061726|ref|XP_010275264.1|3.3e-10752.53PREDICTED: E3 ubiquitin-protein ligase PUB22-like [Nelumbo nucifera][more]
gi|1009110389|ref|XP_015895309.1|8.4e-10348.90PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Ziziphus jujuba][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR003613Ubox_domain
IPR011989ARM-like
IPR013083Znf_RING/FYVE/PHD
IPR016024ARM-type_fold
Vocabulary: Molecular Function
TermDefinition
GO:0004842ubiquitin-protein transferase activity
GO:0005488binding
Vocabulary: Biological Process
TermDefinition
GO:0016567protein ubiquitination
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005575 cellular_component
molecular_function GO:0005488 binding
molecular_function GO:0016874 ligase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh06G012110.1CmaCh06G012110.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainPFAMPF04564U-boxcoord: 55..113
score: 2.
IPR003613U box domainSMARTSM00504Ubox_2coord: 52..112
score: 8.2
IPR003613U box domainPROFILEPS51698U_BOXcoord: 55..119
score: 11
IPR011989Armadillo-like helicalGENE3DG3DSA:1.25.10.10coord: 363..456
score: 5.1E-8coord: 128..284
score: 5.
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 54..113
score: 5.5
IPR016024Armadillo-type foldunknownSSF48371ARM repeatcoord: 354..437
score: 1.34E-9coord: 123..295
score: 1.3
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 356..483
score: 1.6E-140coord: 55..321
score: 1.6E
NoneNo IPR availablePANTHERPTHR22849:SF23SUBFAMILY NOT NAMEDcoord: 356..483
score: 1.6E-140coord: 55..321
score: 1.6E
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 54..115
score: 9.71

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh06G012110CmaCh14G019510Cucurbita maxima (Rimu)cmacmaB289