CmaCh05G009960 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh05G009960 vs. Swiss-Prot
Match: NCASE_ARATH (Neutral ceramidase OS=Arabidopsis thaliana GN=At2g38010 PE=3 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 2.1e-179
Identity = 348/661 (52.65%), Postives = 443/661 (67.02%), Query Frame = 1

Query: 47  YANMDQSAAGIHFR------LRARTFIVAKSVDGPRIAFVNLDAGMASQLVTIKVLERLK 106
           Y   + + +GIH        L+  T+IV  S+   R +F  +  G+   +V  +  E L+
Sbjct: 106 YTEKNVAISGIHTHAGPGGYLQYVTYIVT-SLGFVRQSFDVVVNGIEQSIV--QAHESLR 165

Query: 107 ASSVDV-AGEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGKSLGAFS 166
             S  V  G++ +AG+NRSPS+YL NP  ER++Y  +VD EM+LVK V+   G + G+F+
Sbjct: 166 PGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMTLVKFVDSQLGPT-GSFN 225

Query: 167 WFPTHGTSMSRDNKLISGDNKGAAARFFEDWLAASNR---STRATPSNKS--------DT 226
           WF THGTSMSR N LISGDNKGAAARF EDW     +   S+R  P   S        + 
Sbjct: 226 WFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSSRNIPRRVSTIVSDFSRNR 285

Query: 227 EELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDS 286
           + L+  A T K++ G    K+    ++VR      FV AFCQSN GDV+PN LG FC D+
Sbjct: 286 DRLLDIAATYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDT 345

Query: 287 GKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVNLFTTATEKLSGEIDF 346
           G PCDFN S+C+G + LC GRGPG+PDE  ST+IIGE+QF  AV LF  ATEKL G+I +
Sbjct: 346 GLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGY 405

Query: 347 RHVYLNFTDIEVELD----GDNVVKTCPAALGPGFAAGTTDGHGVFGFQQGDTQINKLWK 406
           +H YL+F++++V +     G   VKTCPAA+G GFAAGTTDG G F F+QGD Q N  W+
Sbjct: 406 QHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWR 465

Query: 407 KLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFT 466
            +R+ LR P   QV CQKPK +LLDTGEM EPY WAP+ILPIQILR+G+L+ILSVPGEFT
Sbjct: 466 LVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFT 525

Query: 467 TMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLY 526
           TMAGRRLR+A+K  LIS+   EF ++ HVVIAGLTN YSQY+AT EEYE QRYE ASTLY
Sbjct: 526 TMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLY 585

Query: 527 GPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDI 586
           G HTL+AYIQEFKKLA A+  G  + +    PPDL   Q+  +S   ++S P GV FGD+
Sbjct: 586 GRHTLTAYIQEFKKLATALVNGLTLPRG-PQPPDLLDKQISLLSPVVVDSTPLGVKFGDV 645

Query: 587 QQDIKLPKVGWFKRGGKQKPTARFWSANPRFDLLTEGTYAVVER-QEKRRWTPVYDDDDF 646
           + D+  P    F+RG  Q+  A FWS  PR DL+TEG++AVVE  +E  +W PVYDDDDF
Sbjct: 646 KADV--PPKSTFRRG--QQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDF 705

Query: 647 SLVFKWALDNNTFTNSLATIEWDIPTDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFV 685
           SL FKW+      + S ATIEW +P  A  GVYR+RH+G+SKS   S +  F+G+SSAFV
Sbjct: 706 SLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASKSLFGSIS-SFSGSSSAFV 756

BLAST of CmaCh05G009960 vs. Swiss-Prot
Match: NCASE_ORYSJ (Neutral ceramidase OS=Oryza sativa subsp. japonica GN=Os01g0624000 PE=1 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 2.0e-174
Identity = 317/607 (52.22%), Postives = 417/607 (68.70%), Query Frame = 1

Query: 108 GEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGKSLGAFSWFPTHGTS 167
           G++ +AG+NRSPSAYL NP EER++Y  NVD EM+L+K V+   G  +G+F+WF THGTS
Sbjct: 186 GDLLDAGVNRSPSAYLNNPAEERSKYEYNVDKEMTLIKFVDDELGP-VGSFNWFATHGTS 245

Query: 168 MSRDNKLISGDNKGAAARFFEDWLAASNRSTRATPSNKSD-------------------- 227
           MSR N LISGDNKGAAARF EDW        ++  +N  D                    
Sbjct: 246 MSRTNSLISGDNKGAAARFMEDWAEQMGLPKQSAHANSDDLRSLHKTSVLPRRVSTIIPE 305

Query: 228 ----TEELMKKAETIKATGGKRCSKTS-SQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLG 287
               T++L++ A + +A+GG+R + +S ++  +  + +   FV AFCQSN GDV+PNVLG
Sbjct: 306 PNEITDDLIQLASSYEASGGRRLAGSSITRRIRSTQQNKPKFVSAFCQSNCGDVSPNVLG 365

Query: 288 AFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVNLFTTATEKL 347
            FC D+  PCDFN S+C+G + LC GRGPG+PDE  ST++IG RQFLKA +LF +A+E++
Sbjct: 366 TFCIDTNLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRVIGNRQFLKARDLFDSASEEI 425

Query: 348 SGEIDFRHVYLNFTDIEVELD----GDNVVKTCPAALGPGFAAGTTDGHGVFGFQQGDTQ 407
            G+ID+RH YL+F+ +EV++     G   VKTCPAA+G  FAAGTTDG G F F+QGD +
Sbjct: 426 QGKIDYRHTYLDFSKLEVKVSTSAGGQQTVKTCPAAMGFAFAAGTTDGPGAFDFRQGDVK 485

Query: 408 INKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILS 467
            N  WK +R+ L+ P + QV C  PK +LLDTGEM EPY WAPAILP+Q++R+G+L+IL 
Sbjct: 486 GNPFWKLVRNLLKTPGKDQVECHSPKPILLDTGEMKEPYDWAPAILPVQMIRIGQLVILC 545

Query: 468 VPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYE 527
           VPGEFTTMAGRRLR+AVK T++++GN EFD + HVV+AGLTN+YSQY+ T EEY+ QRYE
Sbjct: 546 VPGEFTTMAGRRLRDAVK-TVLTSGNSEFDKNIHVVLAGLTNSYSQYITTFEEYQIQRYE 605

Query: 528 AASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNG 587
            ASTLYGPHTLSAYIQEF+KLA+A+   +++      PPD+   Q+  +     +S P G
Sbjct: 606 GASTLYGPHTLSAYIQEFQKLAMAMIANKEVPTNF-QPPDMLDKQIGLLPGVVFDSTPLG 665

Query: 588 VNFGDIQQDIKLPKVGWFKRGGKQKPTARFWSANPRFDLLTEGTYAVVERQE-KRRWTPV 647
           V FGD+  D+  P    F +G     T  F+SA PR DLLT+GT+A+VE+ +    W PV
Sbjct: 666 VKFGDVNSDV--PGNSTFNKGSTVNAT--FYSACPRNDLLTDGTFALVEKLDGNNNWVPV 725

Query: 648 YDDDDFSLVFKWALDNNTFTNSLATIEWDIPTDANPGVYRLRHFGSSKSAINSTNIYFTG 685
           YDDDD+SL FKW+      + S AT+EW +P DA  GVYRLRHFG+SK    S   +FTG
Sbjct: 726 YDDDDWSLRFKWSRPARLSSRSFATLEWTVPEDAAAGVYRLRHFGASKPMFGSVR-HFTG 784

BLAST of CmaCh05G009960 vs. Swiss-Prot
Match: NCSEB_DICDI (Neutral ceramidase B OS=Dictyostelium discoideum GN=dcd2B PE=3 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 4.4e-105
Identity = 235/586 (40.10%), Postives = 327/586 (55.80%), Query Frame = 1

Query: 108 GEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGKSLGAFSWFPTHGTS 167
           GE+ N  INRSP AY  NP EE+A Y +NVD  M+++++ E  +G    A S+F  H TS
Sbjct: 192 GELWNTNINRSPFAYDNNPEEEKAMYDSNVDKNMTVLRI-EDMNGNPFAAISFFAVHCTS 251

Query: 168 MSRDNKLISGDNKGAAARFFEDWLAASNRSTRATPSNKSDTEELMKKAETIKATGGKRCS 227
           M+  N LISGDNKG A+                          L +K      T GK   
Sbjct: 252 MNNTNHLISGDNKGYAS-------------------------YLWEKQVNGPGTAGK--- 311

Query: 228 KTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCV 287
                           FV AF QSN GDV+PN  G  C D G PCD+  S+C+G +  C 
Sbjct: 312 --------------GPFVAAFGQSNEGDVSPNTRGPTCRD-GSPCDYKTSTCNGRNEECW 371

Query: 288 GRGPGFPDEIL-STKIIGERQFLKAVNLFTTATEKLSGEIDFRHVYLNFTDIEVEL---D 347
             GPG   ++  ST+IIG  QF KA+ LF  A+ ++SG + +RH ++ FT++ VE     
Sbjct: 372 SLGPGKDGDMFESTQIIGGNQFNKALELFNNASIQVSGPVQYRHSWVQFTNVSVEPPYNS 431

Query: 348 GDNVVKTCPAALGPGFAAGTTDGHGVFGFQQGD--TQINKLWKKLRDSLRKPSEFQVGCQ 407
           G +   TC  A+G  FAAGTTDG G F F Q D  T  N  W  + D + KP+  Q+ CQ
Sbjct: 432 GVDNATTCRGAMGYSFAAGTTDGPGAFNFVQSDNNTSGNPFWNFIGDFIAKPTPDQIRCQ 491

Query: 408 KPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLIS 467
            PK +LLD G M EP  W P ++PIQI+ +G++++++VPGEFTTM+GRRLR +V+E +  
Sbjct: 492 SPKPILLDVG-MVEPIPWVPDVMPIQIVTIGQIVLVAVPGEFTTMSGRRLRNSVREII-- 551

Query: 468 NGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAI 527
              GE  ++  V+IAGL+N YS Y+AT EEY+ QRYE AST++GPHTL +Y+QEF KLA 
Sbjct: 552 ---GESIENPIVLIAGLSNTYSGYIATFEEYQVQRYEGASTVFGPHTLGSYMQEFGKLAQ 611

Query: 528 AIAKGEKI-AKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGG 587
           +I  G  + A P  +P +L+   L F+    +++ P+  +FG++  D+ L          
Sbjct: 612 SIVDGTTVPAGP--TPRNLTGHTLFFLPPVIVDAAPDFDDFGEVSIDVNL------NYSV 671

Query: 588 KQKPTARFWSANPRFDLLTEGTYAVVE-RQEKRRWTPVYDDDDFSLVFKWALDNNTFTNS 647
            +  +  F+  NPR D + E ++  V+      +WT V DD D+   FKW + +  F  S
Sbjct: 672 NETVSCVFYGGNPRNDFMIESSFLSVDLLTGTDQWTTVLDDGDWDTKFKWKMHDLGF--S 717

Query: 648 LATIEWDIPTDANPGVYRLRHFGSSKSAINSTNIY-FTGASSAFVV 685
           L TIEW I  D  PG YR+ H G +K    S+N+  + G S  FVV
Sbjct: 732 LITIEWVIAPDTTPGTYRITHSGFAKKNPFSSNLTPYQGISRNFVV 717

BLAST of CmaCh05G009960 vs. Swiss-Prot
Match: NCSEA_DICDI (Neutral ceramidase A OS=Dictyostelium discoideum GN=dcd2A PE=1 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 2.5e-92
Identity = 216/584 (36.99%), Postives = 309/584 (52.91%), Query Frame = 1

Query: 108 GEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGKSLGAFSWFPTHGTS 167
           GE+ N+ INRSP AY  NP EE+A Y  NVD  M+++++ +                   
Sbjct: 186 GELWNSNINRSPYAYDNNPEEEKAMYDANVDKNMTVIRIED------------------- 245

Query: 168 MSRDNKLISGDNKGAAARFFEDWLAASNRSTRATPSNKSDTEELMKKAETIKATGGKRCS 227
                  +SG+   A + F     + +N +   +  NK     L +K     A G     
Sbjct: 246 -------MSGNPFAAISFFGVHCTSMNNTNHLISGDNKGYASYLWEK----HANGQSSLP 305

Query: 228 KTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCV 287
            T              F+ AF QSN GDV+PN  G  C D GKPCD+  S+C+G    C 
Sbjct: 306 GTGP------------FIAAFGQSNEGDVSPNTRGPTCRD-GKPCDYKTSTCNGKVEECW 365

Query: 288 GRGPGFPDEIL-STKIIGERQFLKAVNLFTTATEKLSGEIDFRHVYLNFTDIEVELDGDN 347
             GPG   ++  ST+IIG  QF KA+ LF  AT ++SG+I +RH +  FT++ VE   ++
Sbjct: 366 ALGPGTDGDMFESTQIIGGNQFNKALELFNNATIQVSGKIQYRHTWKPFTNVSVEAPYNS 425

Query: 348 VVK---TCPAALGPGFAAGTTDGHGVFGFQQGDTQI-NKLWKKLRDSLRKPSEFQVGCQK 407
            V+   TC  A+G  FA GTTDG G F F QGD    N  W  +   + KP+  Q  CQ 
Sbjct: 426 GVEGATTCRGAMGYSFAGGTTDGPGAFNFIQGDNSTTNPFWNFIGGIIAKPTPQQTACQA 485

Query: 408 PKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISN 467
           PK +L+D G M EP  W P ++P+QI+ LG++++++VPGEFTTM+GRRLR  V+E +   
Sbjct: 486 PKPILIDVG-MVEPIPWVPDVMPLQIITLGQIVLVAVPGEFTTMSGRRLRNTVREII--- 545

Query: 468 GNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIA 527
             G+  ++  V+IAGL N YS Y+AT EE++ QRYE AST++GPHTL AY QEF  LA +
Sbjct: 546 --GQSIENPIVLIAGLANTYSGYIATFEEFQVQRYEGASTVFGPHTLGAYQQEFANLAQS 605

Query: 528 IAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGGKQ 587
           I  G + A P   P ++S     F+    ++  P   +FGDI  D+             Q
Sbjct: 606 IVDGSQ-ADPGTFPRNMSGHTPFFLPPVIVDVAPKFDDFGDIYTDVSTTT----PYSINQ 665

Query: 588 KPTARFWSANPRFDLLTEGTYAVVER-QEKRRWTPVYDDDDFSLVFKWALDNNTFTNSLA 647
             T  F+ AN R + +TE ++  V++ Q   +WT + +D D+     W + +  F  SL 
Sbjct: 666 TVTVIFYGANLRNNFMTESSFLTVDQLQSNGQWTTILNDGDWDTKLYWKMHDLGF--SLI 713

Query: 648 TIEWDIPTDANPGVYRLRHFGSSKSAINSTNI-YFTGASSAFVV 685
           T++W I     PG YR+ H G +K    S N+ ++ G SS F V
Sbjct: 726 TVDWTISPITQPGTYRITHSGYAKKNPFSDNLTFYQGISSNFNV 713

BLAST of CmaCh05G009960 vs. Swiss-Prot
Match: ASAH2_HUMAN (Neutral ceramidase OS=Homo sapiens GN=ASAH2 PE=1 SV=2)

HSP 1 Score: 331.6 bits (849), Expect = 2.0e-89
Identity = 230/635 (36.22%), Postives = 322/635 (50.71%), Query Frame = 1

Query: 79  FVNLDAGMASQLVTIKVLERLKASSVDVA-------------GEVENAGINRSPSAYLMN 138
           FV    G ++Q     V   LK  S+D+A             G V+   INRSP +YL N
Sbjct: 208 FVIASEGFSNQTFQHMVTGILK--SIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQN 267

Query: 139 PPEERARYPTNVDTEMSLVKVVEGGSGKSLGAFSWFPTHGTSMSRDNKLISGDNKGAAAR 198
           P  ERARY +N D EM ++K+V+                          ++GD+ G  + 
Sbjct: 268 PQSERARYSSNTDKEMIVLKMVD--------------------------LNGDDLGLISW 327

Query: 199 FFEDWLAASNRSTRATPSNKSDTEELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSL-- 258
           F    ++ +N +      N      L+++                       KN G L  
Sbjct: 328 FAIHPVSMNNSNHLVNSDNVGYASYLLEQ----------------------EKNKGYLPG 387

Query: 259 ---FVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCH-GSDLLCVGRGPGFPDEILS 318
              FV AF  SN+GDV+PN+LG  C ++G+ CD   S+C  G   +C+ +GPG  D   S
Sbjct: 388 QGPFVAAFASSNLGDVSPNILGPRCINTGESCDNANSTCPIGGPSMCIAKGPG-QDMFDS 447

Query: 319 TKIIGERQFLKAVNLFTTATEKLSGEIDFRHVYLNFTDIEVELDGDNVVKTCPAALGPGF 378
           T+IIG   + +A  L+ +A+++++G +   H +++ TD+ V L+  +  KTC  ALG  F
Sbjct: 448 TQIIGRAMYQRAKELYASASQEVTGPLASAHQWVDMTDVTVWLNSTHASKTCKPALGYSF 507

Query: 379 AAGTTDGHGVFGFQQGDTQINKLWKKLRDS-LRKPSEFQVGCQKPKTVLLDTGEMFEPYA 438
           AAGT DG G   F QG T+ +  W  +RD  L KPSE    C KPK +LL TGE+ +P+ 
Sbjct: 508 AAGTIDGVGGLNFTQGKTEGDPFWDTIRDQILGKPSEEIKECHKPKPILLHTGELSKPHP 567

Query: 439 WAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGL 498
           W P I+ +QI+ LG L I ++PGEFTTM+GRRLREAV+    S+G      +  VVI+GL
Sbjct: 568 WHPDIVDVQIITLGSLAITAIPGEFTTMSGRRLREAVQAEFASHGM----QNMTVVISGL 627

Query: 499 TNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPD 558
            N Y+ Y+ T EEY+ QRYEAAST+YGPHTLSAYIQ F+ LA AIA  + +A     P  
Sbjct: 628 CNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRNLAKAIAT-DTVANLSRGPEP 687

Query: 559 LSSVQLRFVSDP-FIESPPNGVNFGDIQQDIKLP-KVGWFKRGGKQKPTARFWSANPRFD 618
               QL     P  ++  P G  FGD+ Q  K   +VG       +     F  ANP+  
Sbjct: 688 PFFKQLIVPLIPSIVDRAPKGRTFGDVLQPAKPEYRVG-------EVAEVIFVGANPKNS 747

Query: 619 L--LTEGTYAVVERQE--KRRWTPVYDDDDFSLVFKWALDNNTFTNSLATIEWDIPTDAN 678
           +   T  T+  VE+ E     W  V +D  +   F W         S AT+EW IP  A 
Sbjct: 748 VQNQTHQTFLTVEKYEATSTSWQIVCNDASWETRFYW--HKGLLGLSNATVEWHIPDTAQ 777

Query: 679 PGVYRLRHFGSSKS---AINSTNIYFTGASSAFVV 685
           PG+YR+R+FG ++       +  + F G S AF V
Sbjct: 808 PGIYRIRYFGHNRKQDILKPAVILSFEGTSPAFEV 777

BLAST of CmaCh05G009960 vs. TrEMBL
Match: A0A0A0KII3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G361230 PE=4 SV=1)

HSP 1 Score: 1025.8 bits (2651), Expect = 2.4e-296
Identity = 507/577 (87.87%), Postives = 531/577 (92.03%), Query Frame = 1

Query: 109 EVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGKSLGAFSWFPTHGTSM 168
           EVENAGINRSPSAYLMNP EERA+Y  NVD EMS++K V+G +G S+GAFSWFPTHGTSM
Sbjct: 84  EVENAGINRSPSAYLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTSM 143

Query: 169 SRDNKLISGDNKGAAARFFEDWLAASNRSTRATPSNK-SDTEELMKKAETIKATGGKRCS 228
           SRDNKLISGDNKGAAARFFEDW AASNR T  TPSN  S  E+LMKKA  IKATGGKRCS
Sbjct: 144 SRDNKLISGDNKGAAARFFEDW-AASNRRTTTTPSNNTSGIEDLMKKAGKIKATGGKRCS 203

Query: 229 KTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCV 288
           KTSS+SSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSG PCDFN SSCHGSDLLCV
Sbjct: 204 KTSSKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCV 263

Query: 289 GRGPGFPDEILSTKIIGERQFLKAVNLFTTATEKLSGEIDFRHVYLNFTDIEVELDGDNV 348
           GRGPGFPDEILSTKIIGERQFLKA +LFTTATEKL+GEIDFRHVYLNFTDIEV +DG+NV
Sbjct: 264 GRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVAIDGNNV 323

Query: 349 VKTCPAALGPGFAAGTTDGHGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVL 408
           V+TCPAALGPGFAAGTTDG G FGFQQGDT+INKLWK+LRDSL+KPSEFQVGCQKPKTVL
Sbjct: 324 VRTCPAALGPGFAAGTTDGPGAFGFQQGDTEINKLWKQLRDSLKKPSEFQVGCQKPKTVL 383

Query: 409 LDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEF 468
           LDTGEMFEPYAWAPAILPIQILRLG LIILSVPGEFTTMAGRRLREAVKETLISNGNG F
Sbjct: 384 LDTGEMFEPYAWAPAILPIQILRLGNLIILSVPGEFTTMAGRRLREAVKETLISNGNGVF 443

Query: 469 DDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGE 528
           DDDTHVVIAGLTN YSQYVAT EEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGE
Sbjct: 444 DDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGE 503

Query: 529 KIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGGKQKPTAR 588
           KIAKPI SPPDLSSVQLR V DPF ESPPNGVNFGDIQQD++LPK GWFK G KQKPTA 
Sbjct: 504 KIAKPIASPPDLSSVQLRLVMDPFGESPPNGVNFGDIQQDVELPKDGWFKTGSKQKPTAT 563

Query: 589 FWSANPRFDLLTEGTYAVVERQEKRRWTPVYDDDDFSLVFKWALDNNTFTNSLATIEWDI 648
           FWSANPRFDLLTEGTYA+VER EK+RWTP YDDDDFSL FKW LD NTF N+L TIEWDI
Sbjct: 564 FWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSLFFKWKLD-NTFINNLVTIEWDI 623

Query: 649 PTDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFVV 685
           P DA+PGVYRLRHFGSS+S INSTN+YFTGAS AF V
Sbjct: 624 PIDADPGVYRLRHFGSSRSTINSTNVYFTGASRAFAV 658

BLAST of CmaCh05G009960 vs. TrEMBL
Match: V4T5S7_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10003183mg PE=4 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 7.2e-256
Identity = 443/596 (74.33%), Postives = 508/596 (85.23%), Query Frame = 1

Query: 93  IKVLERLKASSVDVA-GEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGS 152
           ++    LK  S+ +  G+VENAGINRSPSAYL+NP EERARYP NVD EM+L+K V+  S
Sbjct: 247 VQAHNNLKPGSIFIRKGDVENAGINRSPSAYLLNPAEERARYPENVDKEMTLLKFVDAAS 306

Query: 153 GKSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWLAASNRSTRATPSNKSDTEEL 212
           GKS+GAF+W+ THGTSMSRDN LISGDNKGAAARFFEDW ++ N S+  T    ++  +L
Sbjct: 307 GKSIGAFNWYATHGTSMSRDNLLISGDNKGAAARFFEDWFSSKNESSSIT----TNIGKL 366

Query: 213 MKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKP 272
           M+K++TIKATGG++C KT+SQ  KVRKNDGSLFVGAFCQSNVGDVTPNVLGAFC DSGKP
Sbjct: 367 MEKSKTIKATGGQQCGKTTSQGFKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCIDSGKP 426

Query: 273 CDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVNLFTTATEKLSGEIDFRHV 332
           CDFN SSCHG D LC+GRGPG+PDEILSTKIIGERQF KAV+LF +AT++LSG+ID+RHV
Sbjct: 427 CDFNHSSCHGDDQLCIGRGPGYPDEILSTKIIGERQFQKAVDLFNSATDQLSGKIDYRHV 486

Query: 333 YLNFTDIEVELDG--DNVVKTCPAALGPGFAAGTTDGHGVFGFQQGDTQINKLWKKLRDS 392
           Y+NFT+IEV LDG  +N VKTCPAALGPGFAAGTTDG GVFGFQQGDT+INKLWK+LRD 
Sbjct: 487 YINFTNIEVGLDGTGNNTVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKLWKQLRDV 546

Query: 393 LRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGR 452
           L+KPS++Q  CQKPK VLL +GEMFEPYAWAPA+LPIQILRLGKLIILSVPGEFTTMAGR
Sbjct: 547 LKKPSQYQEECQKPKAVLLSSGEMFEPYAWAPAVLPIQILRLGKLIILSVPGEFTTMAGR 606

Query: 453 RLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTL 512
           RLREAVK+TLI+NGNGEFD+DTH+VIAGLTNAYSQY+AT EEY  QRYEAASTLYGPHTL
Sbjct: 607 RLREAVKDTLINNGNGEFDNDTHIVIAGLTNAYSQYIATFEEYAHQRYEAASTLYGPHTL 666

Query: 513 SAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIK 572
            AYIQEFKKLA A+AKGEK  K   SPPDLSSVQL    +P  +SPP G  FGDI++DI 
Sbjct: 667 PAYIQEFKKLAQAMAKGEKTTKG-PSPPDLSSVQLSLSLNPTGDSPPAGKMFGDIKEDIN 726

Query: 573 LPKVGWFKRGGKQKPTARFWSANPRFDLLTEGTYAVVERQEKRRWTPVYDDDDFSLVFKW 632
            PK G F +G   +P+A FWSANPR+DLLTEGTYAVVE  + +RW PVYDDDDFSL FKW
Sbjct: 727 QPKDGSFNKG--DRPSATFWSANPRYDLLTEGTYAVVEMLQGKRWIPVYDDDDFSLYFKW 786

Query: 633 ALDNNTFTNSLATIEWDIPTDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFVVS 686
           +L+N +F + LATIEW++PT A PGVYRLRHFGSSK A+NSTN YFTGASSAF VS
Sbjct: 787 SLENTSF-HGLATIEWEVPTKAIPGVYRLRHFGSSKKAVNSTNHYFTGASSAFTVS 834

BLAST of CmaCh05G009960 vs. TrEMBL
Match: A0A067GZK8_CITSI (Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g047902mg PE=4 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 7.2e-256
Identity = 443/596 (74.33%), Postives = 508/596 (85.23%), Query Frame = 1

Query: 93  IKVLERLKASSVDVA-GEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGS 152
           ++    LK  S+ +  G+VENAGINRSPSAYL+NP EERARYP NVD EM+L+K V+  S
Sbjct: 133 VQAHNNLKPGSIFIRKGDVENAGINRSPSAYLLNPAEERARYPENVDKEMTLLKFVDAAS 192

Query: 153 GKSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWLAASNRSTRATPSNKSDTEEL 212
           GKS+GAF+W+ THGTSMSRDN LISGDNKGAAARFFEDW ++ N S+  T    ++  +L
Sbjct: 193 GKSIGAFNWYATHGTSMSRDNLLISGDNKGAAARFFEDWFSSKNESSSIT----TNIGKL 252

Query: 213 MKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKP 272
           M+K++TIKATGG++C KT+SQ  KVRKNDGSLFVGAFCQSNVGDVTPNVLGAFC DSGKP
Sbjct: 253 MEKSKTIKATGGQQCGKTTSQGFKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCIDSGKP 312

Query: 273 CDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVNLFTTATEKLSGEIDFRHV 332
           CDFN SSCHG D LC+GRGPG+PDEILSTKIIGERQF KAV+LF +AT++LSG+ID+RHV
Sbjct: 313 CDFNHSSCHGDDQLCIGRGPGYPDEILSTKIIGERQFQKAVDLFNSATDQLSGKIDYRHV 372

Query: 333 YLNFTDIEVELDG--DNVVKTCPAALGPGFAAGTTDGHGVFGFQQGDTQINKLWKKLRDS 392
           Y+NFT+IEV LDG  +N VKTCPAALGPGFAAGTTDG GVFGFQQGDT+INKLWK+LRD 
Sbjct: 373 YINFTNIEVVLDGTGNNTVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKLWKQLRDV 432

Query: 393 LRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGR 452
           L+KPS++Q  CQKPK VLL +GEMFEPYAWAPA+LPIQILRLGKLIILSVPGEFTTMAGR
Sbjct: 433 LKKPSQYQEECQKPKAVLLSSGEMFEPYAWAPAVLPIQILRLGKLIILSVPGEFTTMAGR 492

Query: 453 RLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTL 512
           RLREAVK+TLI+NGNGEFD+DTH+VIAGLTNAYSQY+AT EEY  QRYEAASTLYGPHTL
Sbjct: 493 RLREAVKDTLINNGNGEFDNDTHIVIAGLTNAYSQYIATFEEYAHQRYEAASTLYGPHTL 552

Query: 513 SAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIK 572
            AYIQEFKKLA A+AKGEK  K   SPPDLSSVQL    +P  +SPP G  FGDI++DI 
Sbjct: 553 PAYIQEFKKLAQAMAKGEKTTKG-PSPPDLSSVQLSLSLNPTGDSPPAGKMFGDIKEDIN 612

Query: 573 LPKVGWFKRGGKQKPTARFWSANPRFDLLTEGTYAVVERQEKRRWTPVYDDDDFSLVFKW 632
            PK G F +G   +P+A FWSANPR+DLLTEGTYAVVE  + +RW PVYDDDDFSL FKW
Sbjct: 613 QPKDGSFNKG--DRPSATFWSANPRYDLLTEGTYAVVEMLQGKRWIPVYDDDDFSLYFKW 672

Query: 633 ALDNNTFTNSLATIEWDIPTDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFVVS 686
           +L+N +F + LATIEW++PT A PGVYRLRHFGSSK A+NSTN YFTGASSAF VS
Sbjct: 673 SLENTSF-HGLATIEWEVPTKAIPGVYRLRHFGSSKKAVNSTNHYFTGASSAFTVS 720

BLAST of CmaCh05G009960 vs. TrEMBL
Match: B9R8J2_RICCO (Ceramidase, putative OS=Ricinus communis GN=RCOM_1600200 PE=4 SV=1)

HSP 1 Score: 876.7 bits (2264), Expect = 1.8e-251
Identity = 431/594 (72.56%), Postives = 501/594 (84.34%), Query Frame = 1

Query: 93  IKVLERLKASSVDV-AGEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGS 152
           I+  + LK  S+ +  G+VE AGINRSPSAYL+NP EERARYP NVD EM+L+K V+  +
Sbjct: 161 IQAHQNLKPGSIFINTGDVETAGINRSPSAYLLNPAEERARYPANVDKEMTLLKFVDSAT 220

Query: 153 GKSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWLAASNRSTRATPSNKSDTEEL 212
           G S+GAFSW+ THGTSMSRDNKLISGDNKGAAARFFEDW  ++N+   A+ SN  D ++L
Sbjct: 221 GISIGAFSWYATHGTSMSRDNKLISGDNKGAAARFFEDWFTSTNKPKSAS-SNSVDIDKL 280

Query: 213 MKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKP 272
           ++KA+ I+ATGG+ C KT+SQ+ KVRKNDGS FVGAFCQSNVGDVTPNVLGAFC+DSGKP
Sbjct: 281 IEKAQKIRATGGQPCGKTTSQAFKVRKNDGSRFVGAFCQSNVGDVTPNVLGAFCSDSGKP 340

Query: 273 CDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVNLFTTATEKLSGEIDFRHV 332
           CDFN SSCHG   LC+GRGPG+PDEILSTKIIGERQF KAV+LF +A+EKLSG+ID+RH+
Sbjct: 341 CDFNHSSCHGDVRLCLGRGPGYPDEILSTKIIGERQFDKAVDLFMSASEKLSGKIDYRHM 400

Query: 333 YLNFTDIEVELDGDNVVKTCPAALGPGFAAGTTDGHGVFGFQQGDTQINKLWKKLRDSLR 392
           YLNFTD+EV+L      KTCPAALGPGFAAGTTDG G+FGF+QGDT+IN+LW+++RD L+
Sbjct: 401 YLNFTDLEVDLGEQGKAKTCPAALGPGFAAGTTDGPGMFGFEQGDTEINELWRQVRDFLK 460

Query: 393 KPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRL 452
           KPS++Q  CQKPK VLL TGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRL
Sbjct: 461 KPSQYQEDCQKPKNVLLSTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRL 520

Query: 453 REAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSA 512
           REAVKETLISNG GEFDD+TH+VIAGLTN YSQYVAT+EEY QQRYEAASTLYGPHTLSA
Sbjct: 521 REAVKETLISNGGGEFDDETHIVIAGLTNTYSQYVATIEEYGQQRYEAASTLYGPHTLSA 580

Query: 513 YIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLP 572
           YIQEF +LA A+AKGE++ K  + PPDLSS QLR + DP+I+SPP G+ FGD++QD+  P
Sbjct: 581 YIQEFNRLATAMAKGEQLPKIDLLPPDLSSKQLRLLLDPWIDSPPKGIKFGDMKQDVTQP 640

Query: 573 KVGWFKRGGKQKPTARFWSANPRFDLLTEGTYAVVERQEKRRWTPVYDDDDFSLVFKWAL 632
           K G FK+G   +PTA FWS NPRFDLLTEGT+AVVE  +  RW PVYDDDDFSL FKW L
Sbjct: 641 KNGSFKKG--DRPTATFWSGNPRFDLLTEGTFAVVEMLQGERWIPVYDDDDFSLYFKWKL 700

Query: 633 DNNTFTNSLATIEWDIPTDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFVVS 686
           DN T  +SLA +EW++P +A  GVYRLRHFGSSK   NS   YFTGASSAF VS
Sbjct: 701 DNKTL-SSLARVEWEVPNEAISGVYRLRHFGSSKKTNNSPVEYFTGASSAFTVS 750

BLAST of CmaCh05G009960 vs. TrEMBL
Match: A0A0R0GS00_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_13G076400 PE=4 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 1.7e-249
Identity = 432/593 (72.85%), Postives = 493/593 (83.14%), Query Frame = 1

Query: 93  IKVLERLKASSVDV-AGEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGS 152
           I+    LK  S+ +  G+V++AGINRSPSAYL NP +ERARYPTNVDT+M+L++ V+G S
Sbjct: 157 IQAHNNLKPGSIFMNTGDVKDAGINRSPSAYLQNPADERARYPTNVDTQMTLMRFVDGAS 216

Query: 153 GKSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWLAASNRSTRATPSNKSDTEEL 212
           GK++GAFSWFPTHGTSMS  NKLISGDNKG AAR FEDW A+ N ST  T S   D  +L
Sbjct: 217 GKNIGAFSWFPTHGTSMSNQNKLISGDNKGVAARLFEDWFASQNNSTN-TNSTVPDIGQL 276

Query: 213 MKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKP 272
           MKKA++IKAT GK C K +SQ+SKVRKNDGSLFVGAFCQSNVGDV+PNVLGAFC DSGKP
Sbjct: 277 MKKAQSIKATRGKDCKKLASQASKVRKNDGSLFVGAFCQSNVGDVSPNVLGAFCIDSGKP 336

Query: 273 CDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVNLFTTATEKLSGEIDFRHV 332
           CDFNRSSCHG+D LCVGRGPG+PDEILSTKIIGERQF  AV LF + +E+LSG+ID+RHV
Sbjct: 337 CDFNRSSCHGNDQLCVGRGPGYPDEILSTKIIGERQFKTAVKLFESTSEELSGKIDYRHV 396

Query: 333 YLNFTDIEVELDGDNVVKTCPAALGPGFAAGTTDGHGVFGFQQGDTQINKLWKKLRDSLR 392
           YLNFTDIEVELD + VVKTCPAALGPGFAAGTTDG G+FGFQQGDT+IN  WK +RD L 
Sbjct: 397 YLNFTDIEVELDSNKVVKTCPAALGPGFAAGTTDGPGLFGFQQGDTKINPFWKNVRDFLT 456

Query: 393 KPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRL 452
           KPS++QV CQ PK VLL TGEMF PY WAPAILPIQILRLGKLIILSVPGE TTMAGRRL
Sbjct: 457 KPSQYQVDCQNPKPVLLSTGEMFYPYPWAPAILPIQILRLGKLIILSVPGELTTMAGRRL 516

Query: 453 REAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSA 512
           REAVKETLIS+ NGEFDD+THVVIAGLTN YSQY+AT EEY+QQRYEAASTLYGPHTLSA
Sbjct: 517 REAVKETLISSSNGEFDDETHVVIAGLTNTYSQYIATFEEYQQQRYEAASTLYGPHTLSA 576

Query: 513 YIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLP 572
           YIQEFKKLA A+A+GE I     SPPDLSSVQ+ F+ DP  ESPP GV FGDI++D+  P
Sbjct: 577 YIQEFKKLAQAMAEGENITIKGPSPPDLSSVQISFLLDPLGESPPKGVKFGDIKEDVAFP 636

Query: 573 KVGWFKRGGKQKPTARFWSANPRFDLLTEGTYAVVERQEKRRWTPVYDDDDFSLVFKWAL 632
           K G+F +G    P+A FWSANPR+DLLTEGT+A VER +  RW  VYDDDD SL FKW +
Sbjct: 637 KRGYFTKG--DTPSATFWSANPRYDLLTEGTFAAVERLQGERWISVYDDDDLSLFFKWKV 696

Query: 633 DNNTFTNSLATIEWDIPTDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFVV 685
           DN++  + LATIEW+IP DA  GVYRL+HFG++++ I S   YFTGASSAF V
Sbjct: 697 DNSSL-HGLATIEWEIPNDAVSGVYRLKHFGATRTTIISPINYFTGASSAFAV 745

BLAST of CmaCh05G009960 vs. TAIR10
Match: AT5G58980.1 (AT5G58980.1 Neutral/alkaline non-lysosomal ceramidase)

HSP 1 Score: 620.5 bits (1599), Expect = 1.2e-177
Identity = 353/729 (48.42%), Postives = 469/729 (64.33%), Query Frame = 1

Query: 14  LAAVSCCCAHGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRARTFIVAKSVD 73
           L  ++C  +  D+L+G+GS+D+TGPAA+VNMMGYANM+Q A+G+HFRLRAR FIVA+   
Sbjct: 17  LLRLTCIFSDSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAFIVAEPYK 76

Query: 74  GPRIAFVNLDAGMASQLVTIKVLERLK--------ASSVDVAGEVENAG----------- 133
             RIAFVNLDAGMASQLVTIKV+ERLK          +V ++G   +AG           
Sbjct: 77  -KRIAFVNLDAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYLQYILYL 136

Query: 134 ------INRSPSAYLMNPPEERARYPTNVDTEMSLV---KVVEGGSGKSLGAFSWFPTHG 193
                 +++S +A +    +   +   N+     L+   ++++ G  +S  A+   P H 
Sbjct: 137 VTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYLNNPAH- 196

Query: 194 TSMSRDNKLISGDNKGAAARFFE-----------DWLAASN-------RSTRATPSNKSD 253
               R       D +    +F +           DW    N        S R   S  SD
Sbjct: 197 ---ERSKYEYDVDKEMTLVKFVDDQWGPVARIMEDWFERENGCRSVDVESPRRVSSIISD 256

Query: 254 --TEELMKKAETIKATGGKRCSKTSSQSSKVRKN----DGSLFVGAFCQSNVGDVTPNVL 313
              ++LM+ A ++ +TGGK  ++ SS + +VR      D   FV AFCQ+N GDV+PNVL
Sbjct: 257 PYDQDLMEMASSLLSTGGKTVTRMSSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVL 316

Query: 314 GAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVNLFTTATEK 373
           GAFC D+G PC+FN+S+C G +  C GRGPG+PDE  ST+IIGERQF KA +LFT A+E+
Sbjct: 317 GAFCIDTGLPCEFNQSTCGGKNEQCYGRGPGYPDEFESTRIIGERQFKKAADLFTKASEE 376

Query: 374 LSGEIDFRHVYLNFTDIEVELDGDN----VVKTCPAALGPGFAAGTTDGHGVFGFQQGDT 433
           + G++D+RH Y++F+ +EV ++G N    VVKTCPAA+G GFAAGTTDG G F F+QGD 
Sbjct: 377 IQGKVDYRHAYVDFSQLEVTINGQNGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDD 436

Query: 434 QINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIIL 493
           Q N  W+ +R+ L+ P+E QV CQ+PK +LLDTGEM +PY WAP+ILP+QILR+G+L+IL
Sbjct: 437 QGNPFWRLVRNLLKNPTEEQVRCQRPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVIL 496

Query: 494 SVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRY 553
            VPGEFTTMAGRRLR+AVK  L    NG    +  VVIAGLTN+YSQY+AT EEY+ QRY
Sbjct: 497 CVPGEFTTMAGRRLRDAVKTVLKEGSNGR---EFSVVIAGLTNSYSQYIATFEEYQVQRY 556

Query: 554 EAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPN 613
           E ASTLYGPHTLS YIQEFKKLA  +   +    P   PPDL   Q+  ++    +  P 
Sbjct: 557 EGASTLYGPHTLSGYIQEFKKLANDLLSAQ-TTDPGPQPPDLLHKQISLLTPVVADMTPI 616

Query: 614 GVNFGDIQQDIKLPKVGWFKRGGKQKPTARFWSANPRFDLLTEGTYAVVER--QEKRRWT 673
           G  FGD+  D+  P++  F++G       +F SANPR DL+TEGT+A+VER  + +  W 
Sbjct: 617 GTAFGDVTSDV--PRLSKFRKGA-DIVRVQFRSANPRNDLMTEGTFALVERWLEGRETWV 676

Query: 674 PVYDDDDFSLVFKWALDNNTFTNSLATIEWDIPTDANPGVYRLRHFGSSKSAINSTNIYF 685
           PVYDDDDF L FKW+      T S ATIEW IP  A+PGVYR+ HFGS+K+ I+S + +F
Sbjct: 677 PVYDDDDFCLRFKWSRPFKLSTQSTATIEWRIPETASPGVYRITHFGSAKTPISSIH-HF 732

BLAST of CmaCh05G009960 vs. TAIR10
Match: AT1G07380.1 (AT1G07380.1 Neutral/alkaline non-lysosomal ceramidase)

HSP 1 Score: 601.7 bits (1550), Expect = 5.8e-172
Identity = 334/671 (49.78%), Postives = 441/671 (65.72%), Query Frame = 1

Query: 47  YANMDQSAAGIHFRLRARTFI-----VAKSVDGPRIAFVNLDAGMASQLVTIKVLERLKA 106
           Y   +   +GIH       ++     +  S+   R +F  L  G+ + +  I+  E L+ 
Sbjct: 117 YTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDALVDGIENSI--IQAHENLRP 176

Query: 107 SSVDVA-GEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGKSLGAFSW 166
            S+ +  GE+ +AG+NRSPSAYL NP +ER+++  NVD EM+L+K V+   G  +G+F+W
Sbjct: 177 GSIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYNVDKEMTLLKFVDDQWGP-VGSFNW 236

Query: 167 FPTHGTSMSRDNKLISGDNKGAAARFFEDWLAASN---------------RSTRATPSNK 226
           F THGTSMSR N LISGDNKGAA+RF EDW   +                R   +   N 
Sbjct: 237 FATHGTSMSRTNSLISGDNKGAASRFMEDWYEQNTAERSYSEEFISDEIPRRVSSLIENH 296

Query: 227 SDTE-ELMKKAETIKATGGKRCSKTSSQSSKVR----KNDGSLFVGAFCQSNVGDVTPNV 286
            D+  EL++ A   ++  GK  ++ SS + +VR    K D   FV AFCQ+N GDV+PNV
Sbjct: 297 QDSHHELLELASYFESQPGKPVTRISSSARRVRSALRKADKPGFVSAFCQTNCGDVSPNV 356

Query: 287 LGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVNLFTTATE 346
           LGAFC D+G PCDFN S+C G + +C GRGPG+PDE  ST+IIGERQF  A+ LF  A+E
Sbjct: 357 LGAFCLDTGLPCDFNHSTCGGKNEMCYGRGPGYPDEFESTRIIGERQFKMALELFNKASE 416

Query: 347 KLSGEIDFRHVYLNFTDIEVEL---DGDN-VVKTCPAALGPGFAAGTTDGHGVFGFQQGD 406
           +L G++D+RHVY++F+ + V L   DG + VVKTCPAA+G  FAAGTTDG G F F QGD
Sbjct: 417 QLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGD 476

Query: 407 TQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLII 466
            + N  W+ +R+ L+ P + Q+ C  PK +LLDTGEM +PY WAP+IL +Q+LR+G+L I
Sbjct: 477 DKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEMTKPYDWAPSILSLQVLRIGQLFI 536

Query: 467 LSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQR 526
           LSVPGEFTTMAGRRLR AVK  L ++GN +   + HVVIAGL N YSQYV T EEY+ QR
Sbjct: 537 LSVPGEFTTMAGRRLRYAVKTQLKNSGNKDLSGEIHVVIAGLANGYSQYVTTFEEYQVQR 596

Query: 527 YEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPP 586
           YE ASTLYGPHTLS YIQEFKKL+ ++     + +P   PPDL   QL F++   +++ P
Sbjct: 597 YEGASTLYGPHTLSGYIQEFKKLSKSLVLDMPV-QPGPQPPDLLDKQLSFLTPVMMDTTP 656

Query: 587 NGVNFGDIQQDIKLPKVGWFKRGGKQKPTARFWSANPRFDLLTEGTYAVVER--QEKRRW 646
           +G +FGD+  D+  PK    KRG  Q  T  F SA PR DLLTEGT+ +VER  Q+ + W
Sbjct: 657 SGDSFGDVISDV--PKNLSLKRGNGQ-VTVVFRSACPRNDLLTEGTFTLVERLEQKDKTW 716

Query: 647 TPVYDDDDFSLVFKWALDNNTFTNSLATIEWDIPTDANPGVYRLRHFGSSKSAINSTNIY 686
           TPVYDDDD  L FKW+      + S AT+EW IP  A+PGVYR+ HFG++K    S + +
Sbjct: 717 TPVYDDDDLCLRFKWSRHKKLSSRSQATVEWRIPESASPGVYRITHFGAAKKLFGSVH-H 776

BLAST of CmaCh05G009960 vs. TAIR10
Match: AT2G38010.2 (AT2G38010.2 Neutral/alkaline non-lysosomal ceramidase)

HSP 1 Score: 531.9 bits (1369), Expect = 5.6e-151
Identity = 274/479 (57.20%), Postives = 340/479 (70.98%), Query Frame = 1

Query: 211 LMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGK 270
           L+  A T K++ G    K+    ++VR      FV AFCQSN GDV+PN LG FC D+G 
Sbjct: 319 LLDIAATYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGL 378

Query: 271 PCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVNLFTTATEKLSGEIDFRH 330
           PCDFN S+C+G + LC GRGPG+PDE  ST+IIGE+QF  AV LF  ATEKL G+I ++H
Sbjct: 379 PCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQH 438

Query: 331 VYLNFTDIEVELD----GDNVVKTCPAALGPGFAAGTTDGHGVFGFQQGDTQINKLWKKL 390
            YL+F++++V +     G   VKTCPAA+G GFAAGTTDG G F F+QGD Q N  W+ +
Sbjct: 439 AYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLV 498

Query: 391 RDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTM 450
           R+ LR P   QV CQKPK +LLDTGEM EPY WAP+ILPIQILR+G+L+ILSVPGEFTTM
Sbjct: 499 RNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTM 558

Query: 451 AGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGP 510
           AGRRLR+A+K  LIS+   EF ++ HVVIAGLTN YSQY+AT EEYE QRYE ASTLYG 
Sbjct: 559 AGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGR 618

Query: 511 HTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQ 570
           HTL+AYIQEFKKLA A+  G  + +    PPDL   Q+  +S   ++S P GV FGD++ 
Sbjct: 619 HTLTAYIQEFKKLATALVNGLTLPRG-PQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKA 678

Query: 571 DIKLPKVGWFKRGGKQKPTARFWSANPRFDLLTEGTYAVVER-QEKRRWTPVYDDDDFSL 630
           D+  P    F+RG  Q+  A FWS  PR DL+TEG++AVVE  +E  +W PVYDDDDFSL
Sbjct: 679 DV--PPKSTFRRG--QQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSL 738

Query: 631 VFKWALDNNTFTNSLATIEWDIPTDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFVV 685
            FKW+      + S ATIEW +P  A  GVYR+RH+G+SKS   S +  F+G+SSAFVV
Sbjct: 739 KFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASKSLFGSIS-SFSGSSSAFVV 791

BLAST of CmaCh05G009960 vs. NCBI nr
Match: gi|659129879|ref|XP_008464893.1| (PREDICTED: neutral ceramidase-like [Cucumis melo])

HSP 1 Score: 1035.0 bits (2675), Expect = 5.8e-299
Identity = 505/577 (87.52%), Postives = 534/577 (92.55%), Query Frame = 1

Query: 108 GEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGKSLGAFSWFPTHGTS 167
           GEV+NAGINRSPSAYLMNP EERA+Y  NVD EMS++K V+G SG S+GAFSWFPTHGTS
Sbjct: 171 GEVDNAGINRSPSAYLMNPEEERAKYANNVDKEMSILKFVDGESGNSIGAFSWFPTHGTS 230

Query: 168 MSRDNKLISGDNKGAAARFFEDWLAASNRSTRATPSNKSDTEELMKKAETIKATGGKRCS 227
           MSRDNKLISGDNKGAAARFFEDW A++ R+T    +N S  EELMKKAE IKATGGK+CS
Sbjct: 231 MSRDNKLISGDNKGAAARFFEDWAASNGRTTTTPSNNASVIEELMKKAEKIKATGGKQCS 290

Query: 228 KTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCV 287
           KTSS+SSKVRKNDGSLFV AFCQSNVGDVTPNVLGAFC DSGKPCDFNRSSCHGSDLLCV
Sbjct: 291 KTSSKSSKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCIDSGKPCDFNRSSCHGSDLLCV 350

Query: 288 GRGPGFPDEILSTKIIGERQFLKAVNLFTTATEKLSGEIDFRHVYLNFTDIEVELDGDNV 347
           GRGPGFPDEILSTKIIGERQFLKA +LFTTATEKL+GEIDFRHVYLNFTDIEVE+DG+NV
Sbjct: 351 GRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNV 410

Query: 348 VKTCPAALGPGFAAGTTDGHGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVL 407
           V+TCPAALGPGFAAGTTDG GVFGFQQGDT+INKLWK+LRDSL+KPSEFQVGCQKPKTVL
Sbjct: 411 VRTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKLWKQLRDSLKKPSEFQVGCQKPKTVL 470

Query: 408 LDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEF 467
           LDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEF
Sbjct: 471 LDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEF 530

Query: 468 DDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGE 527
           DDDTHVVIAGLTN YSQYVAT EEYEQQRYEAASTLYGPHTLSAYIQEFKKLA AIAKGE
Sbjct: 531 DDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLASAIAKGE 590

Query: 528 KIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGGKQKPTAR 587
           K+AKPI SPPDLSSVQLR V DPF ESPP+GVNFGDIQQD++LPK GWFK G KQKPTA 
Sbjct: 591 KVAKPIASPPDLSSVQLRLVMDPFGESPPDGVNFGDIQQDVELPKDGWFKTGSKQKPTAT 650

Query: 588 FWSANPRFDLLTEGTYAVVERQEKRRWTPVYDDDDFSLVFKWALDNNTFTNSLATIEWDI 647
           FWSANPRFDLLTEGTYA+VER EK+RWTP YDDDDFS+ FKW LDN T  NSLATIEWDI
Sbjct: 651 FWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSVSFKWKLDNTTLINSLATIEWDI 710

Query: 648 PTDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFVV 685
           P DA+PGVYRLRHFGSS+S INSTNIYFTGAS AF V
Sbjct: 711 PIDADPGVYRLRHFGSSRSTINSTNIYFTGASRAFAV 747

BLAST of CmaCh05G009960 vs. NCBI nr
Match: gi|449453075|ref|XP_004144284.1| (PREDICTED: neutral ceramidase-like [Cucumis sativus])

HSP 1 Score: 1028.1 bits (2657), Expect = 7.1e-297
Identity = 508/578 (87.89%), Postives = 532/578 (92.04%), Query Frame = 1

Query: 108 GEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGKSLGAFSWFPTHGTS 167
           GEVENAGINRSPSAYLMNP EERA+Y  NVD EMS++K V+G +G S+GAFSWFPTHGTS
Sbjct: 162 GEVENAGINRSPSAYLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTS 221

Query: 168 MSRDNKLISGDNKGAAARFFEDWLAASNRSTRATPSNK-SDTEELMKKAETIKATGGKRC 227
           MSRDNKLISGDNKGAAARFFEDW AASNR T  TPSN  S  E+LMKKA  IKATGGKRC
Sbjct: 222 MSRDNKLISGDNKGAAARFFEDW-AASNRRTTTTPSNNTSGIEDLMKKAGKIKATGGKRC 281

Query: 228 SKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLC 287
           SKTSS+SSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSG PCDFN SSCHGSDLLC
Sbjct: 282 SKTSSKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLC 341

Query: 288 VGRGPGFPDEILSTKIIGERQFLKAVNLFTTATEKLSGEIDFRHVYLNFTDIEVELDGDN 347
           VGRGPGFPDEILSTKIIGERQFLKA +LFTTATEKL+GEIDFRHVYLNFTDIEV +DG+N
Sbjct: 342 VGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVAIDGNN 401

Query: 348 VVKTCPAALGPGFAAGTTDGHGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTV 407
           VV+TCPAALGPGFAAGTTDG G FGFQQGDT+INKLWK+LRDSL+KPSEFQVGCQKPKTV
Sbjct: 402 VVRTCPAALGPGFAAGTTDGPGAFGFQQGDTEINKLWKQLRDSLKKPSEFQVGCQKPKTV 461

Query: 408 LLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGE 467
           LLDTGEMFEPYAWAPAILPIQILRLG LIILSVPGEFTTMAGRRLREAVKETLISNGNG 
Sbjct: 462 LLDTGEMFEPYAWAPAILPIQILRLGNLIILSVPGEFTTMAGRRLREAVKETLISNGNGV 521

Query: 468 FDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKG 527
           FDDDTHVVIAGLTN YSQYVAT EEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKG
Sbjct: 522 FDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKG 581

Query: 528 EKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGGKQKPTA 587
           EKIAKPI SPPDLSSVQLR V DPF ESPPNGVNFGDIQQD++LPK GWFK G KQKPTA
Sbjct: 582 EKIAKPIASPPDLSSVQLRLVMDPFGESPPNGVNFGDIQQDVELPKDGWFKTGSKQKPTA 641

Query: 588 RFWSANPRFDLLTEGTYAVVERQEKRRWTPVYDDDDFSLVFKWALDNNTFTNSLATIEWD 647
            FWSANPRFDLLTEGTYA+VER EK+RWTP YDDDDFSL FKW LD NTF N+L TIEWD
Sbjct: 642 TFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSLFFKWKLD-NTFINNLVTIEWD 701

Query: 648 IPTDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFVV 685
           IP DA+PGVYRLRHFGSS+S INSTN+YFTGAS AF V
Sbjct: 702 IPIDADPGVYRLRHFGSSRSTINSTNVYFTGASRAFAV 737

BLAST of CmaCh05G009960 vs. NCBI nr
Match: gi|700192357|gb|KGN47561.1| (hypothetical protein Csa_6G361230 [Cucumis sativus])

HSP 1 Score: 1025.8 bits (2651), Expect = 3.5e-296
Identity = 507/577 (87.87%), Postives = 531/577 (92.03%), Query Frame = 1

Query: 109 EVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGKSLGAFSWFPTHGTSM 168
           EVENAGINRSPSAYLMNP EERA+Y  NVD EMS++K V+G +G S+GAFSWFPTHGTSM
Sbjct: 84  EVENAGINRSPSAYLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTSM 143

Query: 169 SRDNKLISGDNKGAAARFFEDWLAASNRSTRATPSNK-SDTEELMKKAETIKATGGKRCS 228
           SRDNKLISGDNKGAAARFFEDW AASNR T  TPSN  S  E+LMKKA  IKATGGKRCS
Sbjct: 144 SRDNKLISGDNKGAAARFFEDW-AASNRRTTTTPSNNTSGIEDLMKKAGKIKATGGKRCS 203

Query: 229 KTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCV 288
           KTSS+SSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSG PCDFN SSCHGSDLLCV
Sbjct: 204 KTSSKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCV 263

Query: 289 GRGPGFPDEILSTKIIGERQFLKAVNLFTTATEKLSGEIDFRHVYLNFTDIEVELDGDNV 348
           GRGPGFPDEILSTKIIGERQFLKA +LFTTATEKL+GEIDFRHVYLNFTDIEV +DG+NV
Sbjct: 264 GRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVAIDGNNV 323

Query: 349 VKTCPAALGPGFAAGTTDGHGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVL 408
           V+TCPAALGPGFAAGTTDG G FGFQQGDT+INKLWK+LRDSL+KPSEFQVGCQKPKTVL
Sbjct: 324 VRTCPAALGPGFAAGTTDGPGAFGFQQGDTEINKLWKQLRDSLKKPSEFQVGCQKPKTVL 383

Query: 409 LDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEF 468
           LDTGEMFEPYAWAPAILPIQILRLG LIILSVPGEFTTMAGRRLREAVKETLISNGNG F
Sbjct: 384 LDTGEMFEPYAWAPAILPIQILRLGNLIILSVPGEFTTMAGRRLREAVKETLISNGNGVF 443

Query: 469 DDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGE 528
           DDDTHVVIAGLTN YSQYVAT EEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGE
Sbjct: 444 DDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGE 503

Query: 529 KIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGGKQKPTAR 588
           KIAKPI SPPDLSSVQLR V DPF ESPPNGVNFGDIQQD++LPK GWFK G KQKPTA 
Sbjct: 504 KIAKPIASPPDLSSVQLRLVMDPFGESPPNGVNFGDIQQDVELPKDGWFKTGSKQKPTAT 563

Query: 589 FWSANPRFDLLTEGTYAVVERQEKRRWTPVYDDDDFSLVFKWALDNNTFTNSLATIEWDI 648
           FWSANPRFDLLTEGTYA+VER EK+RWTP YDDDDFSL FKW LD NTF N+L TIEWDI
Sbjct: 564 FWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSLFFKWKLD-NTFINNLVTIEWDI 623

Query: 649 PTDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFVV 685
           P DA+PGVYRLRHFGSS+S INSTN+YFTGAS AF V
Sbjct: 624 PIDADPGVYRLRHFGSSRSTINSTNVYFTGASRAFAV 658

BLAST of CmaCh05G009960 vs. NCBI nr
Match: gi|568839895|ref|XP_006473911.1| (PREDICTED: neutral ceramidase-like [Citrus sinensis])

HSP 1 Score: 896.0 bits (2314), Expect = 4.2e-257
Identity = 446/599 (74.46%), Postives = 509/599 (84.97%), Query Frame = 1

Query: 93  IKVLERLKASSVDVA-GEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGS 152
           ++    LK  S+ +  G+VENAGINRSPSAYL+NP EERARYP NVD EM+L+K V+  S
Sbjct: 162 VQAHNNLKPGSIFIRKGDVENAGINRSPSAYLLNPAEERARYPENVDKEMTLLKFVDAAS 221

Query: 153 GKSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWLAASNRSTRATPSN---KSDT 212
           GKS+GAF+W+ THGTSMSRDN LISGDNKGAAARFFEDW ++ N S+  T S     SD 
Sbjct: 222 GKSIGAFNWYATHGTSMSRDNLLISGDNKGAAARFFEDWFSSKNESSSTTTSKFSTSSDI 281

Query: 213 EELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDS 272
            +LM+K++TIKATGG++C KT+SQ  KVRKNDGSLFVGAFCQSNVGDVTPNVLGAFC DS
Sbjct: 282 GKLMEKSKTIKATGGQQCGKTTSQGFKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCIDS 341

Query: 273 GKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVNLFTTATEKLSGEIDF 332
           GKPCDFN SSCHG D LC+GRGPG+PDEILSTKIIGERQF KAV+LF +AT++LSG+ID+
Sbjct: 342 GKPCDFNHSSCHGDDQLCIGRGPGYPDEILSTKIIGERQFQKAVDLFNSATDQLSGKIDY 401

Query: 333 RHVYLNFTDIEVELDG--DNVVKTCPAALGPGFAAGTTDGHGVFGFQQGDTQINKLWKKL 392
           RHVY+NFT+IEV LDG  +N VKTCPAALGPGFAAGTTDG GVFGFQQGDT+INKLWK+L
Sbjct: 402 RHVYINFTNIEVVLDGTGNNTVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKLWKQL 461

Query: 393 RDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTM 452
           RD L+KPS++Q  CQKPK VLL +GEMFEPYAWAPA+LPIQILRLGKLIILSVPGEFTTM
Sbjct: 462 RDVLKKPSQYQEECQKPKAVLLSSGEMFEPYAWAPAVLPIQILRLGKLIILSVPGEFTTM 521

Query: 453 AGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGP 512
           AGRRLREAVK+TLI+NGNGEFD+DTH+VIAGLTNAYSQY+AT EEY  QRYEAASTLYGP
Sbjct: 522 AGRRLREAVKDTLINNGNGEFDNDTHIVIAGLTNAYSQYIATFEEYAHQRYEAASTLYGP 581

Query: 513 HTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQ 572
           HTL AYIQEFKKLA A+AKGEK  K   SPPDLSSVQL    +P  +SPP G  FGDI++
Sbjct: 582 HTLPAYIQEFKKLAQAMAKGEKTTKG-PSPPDLSSVQLSLSLNPTGDSPPAGKMFGDIKE 641

Query: 573 DIKLPKVGWFKRGGKQKPTARFWSANPRFDLLTEGTYAVVERQEKRRWTPVYDDDDFSLV 632
           DI  PK G F +G   +P+A FWSANPR+DLLTEGTYAVVE  + +RW PVYDDDDFSL 
Sbjct: 642 DINQPKDGSFNKG--DRPSATFWSANPRYDLLTEGTYAVVEMLQGKRWIPVYDDDDFSLY 701

Query: 633 FKWALDNNTFTNSLATIEWDIPTDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFVVS 686
           FKW+L+N +F + LATIEW++PT A PGVYRLRHFGSSK A+NSTN YFTGASSAF VS
Sbjct: 702 FKWSLENTSF-HGLATIEWEVPTKAIPGVYRLRHFGSSKKAVNSTNHYFTGASSAFTVS 756

BLAST of CmaCh05G009960 vs. NCBI nr
Match: gi|641866349|gb|KDO85034.1| (hypothetical protein CISIN_1g047902mg, partial [Citrus sinensis])

HSP 1 Score: 891.3 bits (2302), Expect = 1.0e-255
Identity = 443/596 (74.33%), Postives = 508/596 (85.23%), Query Frame = 1

Query: 93  IKVLERLKASSVDVA-GEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGS 152
           ++    LK  S+ +  G+VENAGINRSPSAYL+NP EERARYP NVD EM+L+K V+  S
Sbjct: 133 VQAHNNLKPGSIFIRKGDVENAGINRSPSAYLLNPAEERARYPENVDKEMTLLKFVDAAS 192

Query: 153 GKSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWLAASNRSTRATPSNKSDTEEL 212
           GKS+GAF+W+ THGTSMSRDN LISGDNKGAAARFFEDW ++ N S+  T    ++  +L
Sbjct: 193 GKSIGAFNWYATHGTSMSRDNLLISGDNKGAAARFFEDWFSSKNESSSIT----TNIGKL 252

Query: 213 MKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKP 272
           M+K++TIKATGG++C KT+SQ  KVRKNDGSLFVGAFCQSNVGDVTPNVLGAFC DSGKP
Sbjct: 253 MEKSKTIKATGGQQCGKTTSQGFKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCIDSGKP 312

Query: 273 CDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVNLFTTATEKLSGEIDFRHV 332
           CDFN SSCHG D LC+GRGPG+PDEILSTKIIGERQF KAV+LF +AT++LSG+ID+RHV
Sbjct: 313 CDFNHSSCHGDDQLCIGRGPGYPDEILSTKIIGERQFQKAVDLFNSATDQLSGKIDYRHV 372

Query: 333 YLNFTDIEVELDG--DNVVKTCPAALGPGFAAGTTDGHGVFGFQQGDTQINKLWKKLRDS 392
           Y+NFT+IEV LDG  +N VKTCPAALGPGFAAGTTDG GVFGFQQGDT+INKLWK+LRD 
Sbjct: 373 YINFTNIEVVLDGTGNNTVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKLWKQLRDV 432

Query: 393 LRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGR 452
           L+KPS++Q  CQKPK VLL +GEMFEPYAWAPA+LPIQILRLGKLIILSVPGEFTTMAGR
Sbjct: 433 LKKPSQYQEECQKPKAVLLSSGEMFEPYAWAPAVLPIQILRLGKLIILSVPGEFTTMAGR 492

Query: 453 RLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTL 512
           RLREAVK+TLI+NGNGEFD+DTH+VIAGLTNAYSQY+AT EEY  QRYEAASTLYGPHTL
Sbjct: 493 RLREAVKDTLINNGNGEFDNDTHIVIAGLTNAYSQYIATFEEYAHQRYEAASTLYGPHTL 552

Query: 513 SAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIK 572
            AYIQEFKKLA A+AKGEK  K   SPPDLSSVQL    +P  +SPP G  FGDI++DI 
Sbjct: 553 PAYIQEFKKLAQAMAKGEKTTKG-PSPPDLSSVQLSLSLNPTGDSPPAGKMFGDIKEDIN 612

Query: 573 LPKVGWFKRGGKQKPTARFWSANPRFDLLTEGTYAVVERQEKRRWTPVYDDDDFSLVFKW 632
            PK G F +G   +P+A FWSANPR+DLLTEGTYAVVE  + +RW PVYDDDDFSL FKW
Sbjct: 613 QPKDGSFNKG--DRPSATFWSANPRYDLLTEGTYAVVEMLQGKRWIPVYDDDDFSLYFKW 672

Query: 633 ALDNNTFTNSLATIEWDIPTDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFVVS 686
           +L+N +F + LATIEW++PT A PGVYRLRHFGSSK A+NSTN YFTGASSAF VS
Sbjct: 673 SLENTSF-HGLATIEWEVPTKAIPGVYRLRHFGSSKKAVNSTNHYFTGASSAFTVS 720