Cp4.1LG11g08010 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG11g08010
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionNeutral ceramidase
LocationCp4.1LG11 : 6516233 .. 6522417 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGGCGGCAGTTGTTGAAGGTTGTGGCCACTGCTGTGCTTGCAGCTGTGAGCTGCTGCTGTGCTGATGGGGATTGGTTGGTCGGCGTCGGGAGCTTCGACATGACTGGCCCGGCTGCCGAAGTGAACATGATGGGGTATGCTAATATGGATCAGAGCGCTGCGGGGATTCATTTTAGGCTCCGAGCGAGGACGTTTATTGTTGCGAAGAGCGTCGATGGGCCGAGAATTGCATTTGTTAATTTAGATGCTGGCATGGCTTCGCAATTGGTCACTATTAAGCTTCTCGAGAGGCTCAAAGCTAGGTATATATATAAGATACGTTAATAATAAATGCCTATTTGTTAGAAACCACGAACTTTCTCAATGGTATGATACTGTCCACTTTGAACATAAGTTTTCATGACTTTTCTCTTGGTTTCCCCAAAATTTCTTATTCTTTACTTATAAACTCATGATTAACCTCTTAATTAGCCGACGTGGGACTCCTCTCCCAACAATCCTCCCCTCGAACAAAGTACTCCCCTGAAGCTTATGGAGTCCTCGAACAGCCTCTCCTTAATCGAGACTTGACTCCTTTCTCTAGAGCCCTCGAACAAAGTACACTCTTTGTTCGACACTTGAGTCACTTTTGGCTCGAACAAAGTACACCATAGAGCCTCCTCTGATGTCTCTGGAGCTCTCGAACAACCTCCCCTTAATTGAGGTTTGACTCCTTTCTCTAGAACCCTCGAATAAAGTACACAATTTGTTTGACACTGGAGTCACTTTTGACTATACCTTCGAGACTCACAACTTCTTTGTTATGTAACGATTCTATCGACATGACTCTAATACCATACTAGAAACCACGAACCTCCACAATGGTATGATATTGTCCACTTTGAACATAAGCTCTCGTGACTTTGTTTTTTTATTTCTCCAAAAGACCTCGTACCAACCGAGAAAGTATTCCTTACCTGTAAAACTATGATCAACCCCTTAATTAGCAAACGTGGGACTCCTCTTCCAACAATCCTCAATACTCTTAACAATATCGATAAAGATAATTATTTTCATTACCTTACATTTTTGTCGAAACTAAAACCATGACATTTCGTACAAAGGTTCGGGAATCTATACAGAGAAGAAAACATAGCAATAAGCGGAATCCACACACACGCCGGCCCAGGCGGCTACTTACAATACCTAGTCTACTCCATAACATCACTTGGCTTCGTTCAACAATCCTTCGACGCGATCGTGAACGCAATCGAACAAAGCATCGTCCAAGCTCACGAAAGCCTAAAACCAGGGTTCATGTTAATCAACAAAGGTAAGAAGAGCATGGTTGAATAATGATTAACTTATGGATGGGGTTGTTGAATGAAAGAAGTAGTGTGGATGTTGCAGGGGAAGTGGAGAATGCGGGGATTAATAGAAGCCCAAGTGCTTACCTTATGAACCCACCGGAGGAACGAGCTCGATATCCAACCAATGTGGACACAGACATGAGCCTTGTGAAGGTGGTGGAGGGAGGCAGTGGTGTGAGCCTTGGCGCCTTCAGCTGGTTCCCCACGCATGGGACGTCGATGAGTAGAGATAATAAGCTCATTAGTGGGGATAACAAGGGCGCTGCTGCCAGGTTTTTTGAGGACTGGGTCGCCGCCGCCTCTAACCGTTCTACCAGAGCCACCTCCTCCAATAAGTCAGGTCAATTGGAAAAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNATACCAATAAATTTTGTATTGGGTTTGAGAAAAATACCTTTAAATTTTAAAATATTTTAAAAATTTTAAGAACATCCCTCGACTTTCAAAAAGTGTTTAAAAATGTCTTCCAACACCTTCCCTCGAACAAAGTATTCTTCTTCGAGGAGGGTCGACTCCTCTTCTTTTGGAGTCCTTTATTAGCACGACTAAGTTTAGGGCATGGCTCTGATACCATGTTAGACGAACACGACTCTCGACAATAGTATGATATTCTCCACTTTGAGCATATGCTCTCATGACTTTGCTTTGGGCTCCCCAAAAGGGGCCTCATATCAATAGAGATAGTATTCCTTGATTATAAACCCACGATCATTCCCTAAATTAGCCGATGTGGGACTTTCATTATCCAACACCTCCCCTTAATTGAGACTCGACTCTTTGTCTTAGTCATTTTTTACTATGCCTTCGAGGAGGCTCGACTACTTTTCCTTTGGAGTCATTTGTTCGATATTTGAGGATTTCCCAATCTATTGGCACGACTAAGTTTAGGGCATGACTCTGATACCATGTTAGACGAACACGACTCTCGACAATAGTATGATATTGTTCACTTTGAGCATATGCTCTCATGACTTTGCTTTGGGCTCCCCAAAAGGGGCCTCATATCAATAGAGATAGTATTCCTTGATTATAAACCCACGATCATTCCCTAAATTAGCCGATGTGGGACTTTCATTATCCAACACCTCCCCTTAATTGAGACTCGACTCTTTGTCTTAGTCATTTTTTACTATGCCTTCGAGGAGGCTCGACTACTTTTCCTTTGGAGTCATTTGTTCGATATTTGAGGATTTCCCAATCTATTGGCACGACTAAGTTTAGGGCATGACTCTGATACCATGTTAGACGAACACGACTCTCGACAATAGTATGATATTGTTCACTTTGAGCATATTCTCTCATGGCGTTGCTTTGGGGTTCCCAAAAGGCCTCTGCCAATGAAGAGAGTATTGTGAGTTGTCAAAAATTTCTATTGTAACTTTTAAAAAAATAACATTAAGCTTCATTTAAGAAATAATTATTAACTTTGAAAAATAATACTAATACTCTTAAACTTTTCGGGAAAAAAAAATAACTTTTTTACATAGTATTTGTAATGAAGGTTAAGGATATTAATACTAATTTTAAAAGATTATGGATATTTTTTAACTGTGGTATTAATATTTTTTGAAATTGAAGAGTATTAATAAAAAAAAAATTAAAGCTATTTCTTTTAACATTTTTGAAATAGAGTATTATTGAAACTTTTAAATGTTTATGAATATGTTTGAAACAAAATACAAAATTTACAGGTATATTTATTAATTTAACCTATTTAATAAGTTATGTTTGTTGGATGATGATGAAAGTCCCGGCTAATTTAGGGAATGATATGGGTTTATAATCAACGAATACTATATTCATTGGTATGAGCCTTTTGGAGAAGCCCAAAGCAAAATCACGAGAACTTATGCTCAAAGTGGATAATATCATCCCATTGTGAAGAGTCATGTTCGTCTAACTTGGTATCAGAGTCATCCCTAAACTTAGTCGTGCCAATAGATTGGTAAATCCTCAAATGTCGAACAAAGGACTCAAAAGAAAAGGAAAATGAGTCGAGACTCGTTTAACTATGCCTATGAGTTACGACCAAAATATATCAATTCTATGAACTCTTTTACTAATTGGGTTGAATCCAACGGACTGTTTTTCAAAAAATTATTCGATGAGGTCGCTATAATATCGTCATCTAAAGTAATAGTTGACCCATTCTAAAGGATTTTACAAATTTAAGTGTATTTGTTCCTAATAAGATTGTTTGAAACAATTAATTTCTTTCTTTTTGAAAATAGACATTGTGGAGTTGATGAAGAAAGCAGAGACGATAAAGGCGACCGGAGGGAAACGATGCAGCAAAACGAGTAGCCAATCATCAAAAGTGAGAAAGAACGACGGATCTCTGTTCGTCGGAGCATTTTGCCAATCCAACGTCGGCGATGTCACGCCCAATGTTCTTGGAGCCTTTTGCACCGATTCCGGCAAGCCCTGCGATTTCAATCGCTCCTCTTGCCATGGCAGTGATCTTCTATGCGTGGGTCGTGGGCCTGGGTAAGCTACGTACAATCATTTCTTTCTTTTGAATCAATGAAGGTTGTGCACGTCAAGTGTTTGTGAAAATGCCACACAGGTTTCCAGATGAAATTCTAAGCACGAAGATCATTGGGGAGAGGCAGTTCTTGAAAGCGGTTGATTTGTTCACAACAGCAACGGAGAGGCTAACTGGGGAGATTGATTTTCGCCATGTGTATCTGAATTTCACGGATATTGAAGTGGAATTGGGTGGGGATAATGTTGTCAAGACATGTCCAGCTGCTCTTGGCCCTGGTTTTGCTGCCGGAACTACGGATGGCCCTGGTGTGTTTGGCTTTCAACAGGGCGATACTCAGGTCAGTACTATGCGTAATGGGCTGAATCTTTGCCTTTTTTTTCTTTTGTCATCCTTGGAAATTTTTTGATTTTGTTGCAGATCAATAAGTTGTGGAAGAAGTTGCGAGATTCATTGAGGAAACCCAGTGAGTTTCAGGTTGGGTGTCAGAAGCCGAAGACGGTGTTGTTGGACACGGGCGAGATGTTCGAACCTTATGCTTGGGCGGTATGAAAGAAATAGTTTGGCTTAAATCAAACATATTTTTGCTTGCTTGGCTTCATTAAGTTTATAGCTCACCTATCCATTCCTTGCATCGCATCGTGCCAACATATCATTTATTAGTCCAAACAATATATCACACGTGTTAGGATGCACAACTCAAACACTTTATTTAGATGAACACAAAAAATAAGAGAGAGAACATGTAAGGAGGAATATTGACTTGAGGTTTATATTCATGACTAAATGTACAGAACAGTGAATAATGCAAAAAATACATACATCCTCAAAAGCTACTATATATAGTTCAGTTTACTATATATAGTCGTCTATAATACTGTAAGATCCCACCTCGGTTAGAGAGGAGAACGAAAAACATTCCTTATAAGGGTGTGGAAATCTCTCCCTAACTAACTCGTTTTAAAACCTTGAGGGGAAGTCTTTGAAAGAGAAGCTCAAAGAGGACAATATCTGCTAACGGTGAGTTTGAGCTATTACAAATAGTATAAAGCCAAACATTGGGAGGTGTGCCAGTGATGACGCTAAGCCCCCAAGGGGGTGGATTGTGAGATCTCACATCGGTTAGAGAGGGAAACGAAACATTTATTATAAAGGTGTGGAGACCTCTCCCTAATAGACGGGTTTTAAAACCTTGAGGAGAAGCCCATAAGAAAAAACCCAAAGAGGACAATATTTGCTAGCGGTGAGATTGGGTATATACTCCAAGCACTGCCTACTAGTGCAAAAATCCATGGATGTGGTGTGCAAAAATGCTTATTGTAATTGATTGCTAACTTTGACGGGTGAATCTGCAGCCAGCAATTCTTCCAATTCAAATTCTCAGGCTAGGAAAACTGATCATACTCTCAGTACCAGGAGGTACAAATAGAAAACAAAGACCCTATCAATTCAAGTTTTTCACTCAGTGTAACATTATTAACCCTTTGTTTTGCAGAGTTCACAACAATGGCGGGGCGTCGACTAAGGGAAGCAGTAAAGGAAACTCTGATTAGCAATGGAAATGGAGAGTTTGATGATGATACCCACGTTGTGATTGCAGGGCTTACAAATGCATACTCCCAATATGTCGCTACTTTAGAAGAGTATGAACAACAACGATATGAAGTAAGCACTTGGAAGGCTAGTTTTTGTCTTACAATTCTTCCTAAGGCTGGTTTTTGTTTCATTACATGTTCCCATGTTATCTTCAGGCTGCCTCAACTCTCTATGGACCACACACTCTATCAGCATATATTCAAGAGTTCAAGAAACTAGCAATAGCAATAGCCAAAGGTGAAAAGATTGCCAAGCCAATCGTCTCACCGCCAGACCTTTCCTCCGTCCAACTCCGATTCGTATCAGACCCTTTCATAGAATCTCCACCGAACGGTGTCAACTTTGGAGACATTCAACAAGACATCAAATTACCAAAAGTTGGTTGGTTCAAAAGAGGGAGCAAACAGAAACCAACTGCCAGATTTTGGAGTGCAAACCCGAGATTCGATCTCTTGACCGAAGGGACCTATGCTGTGGTTGAAAGGCTAGAGAAACGGCGGTGGACACCGGTTTATGATGACGATGATTTCTCCTTGGTTTTTAAATGGGCATTAGACAACAACACATTCACCAAGAGCTTAGCGACTATCGAATGGGACATCCCAATAGACGCCAATCCAGGTGTGTATCGGTTACGACACTTCGGTTCATCAAAGAGTGCAATAAACTCAACCAACATATATTTCACTGGGGCTTCTAGTGCATTTGCAGTGTTCTAA

mRNA sequence

ATGCGGCGGCAGTTGTTGAAGGTTGTGGCCACTGCTGTGCTTGCAGCTGTGAGCTGCTGCTGTGCTGATGGGGATTGGTTGGTCGGCGTCGGGAGCTTCGACATGACTGGCCCGGCTGCCGAAGTGAACATGATGGGGTTCGGGAATCTATACAGAGAAGAAAACATAGCAATAAGCGGAATCCACACACACGCCGGCCCAGGCGGCTACTTACAATACCTAGTCTACTCCATAACATCACTTGGCTTCGTTCAACAATCCTTCGACGCGATCGTGAACGCAATCGAACAAAGCATCGTCCAAGCTCACGAAAGCCTAAAACCAGGGTTCATGTTAATCAACAAAGGGGAAGTGGAGAATGCGGGGATTAATAGAAGCCCAAGTGCTTACCTTATGAACCCACCGGAGGAACGAGCTCGATATCCAACCAATGTGGACACAGACATGAGCCTTGTGAAGGTGGTGGAGGGAGGCAGTGGTGTGAGCCTTGGCGCCTTCAGCTGGTTCCCCACGCATGGGACGTCGATGAGTAGAGATAATAAGCTCATTAGTGGGGATAACAAGGGCGCTGCTGCCAGGTTTTTTGAGGACTGGGTCGCCGCCGCCTCTAACCGTTCTACCAGAGCCACCTCCTCCAATAAGTCAGACATTGTGGAGTTGATGAAGAAAGCAGAGACGATAAAGGCGACCGGAGGGAAACGATGCAGCAAAACGAGTAGCCAATCATCAAAAGTGAGAAAGAACGACGGATCTCTGTTCGTCGGAGCATTTTGCCAATCCAACGTCGGCGATGTCACGCCCAATGTTCTTGGAGCCTTTTGCACCGATTCCGGCAAGCCCTGCGATTTCAATCGCTCCTCTTGCCATGGCAGTGATCTTCTATGCGTGGGTCGTGGGCCTGGGTTTCCAGATGAAATTCTAAGCACGAAGATCATTGGGGAGAGGCAGTTCTTGAAAGCGGTTGATTTGTTCACAACAGCAACGGAGAGGCTAACTGGGGAGATTGATTTTCGCCATGTGTATCTGAATTTCACGGATATTGAAGTGGAATTGGGTGGGGATAATGTTGTCAAGACATGTCCAGCTGCTCTTGGCCCTGGTTTTGCTGCCGGAACTACGGATGGCCCTGGTGTGTTTGGCTTTCAACAGGGCGATACTCAGATCAATAAGTTGTGGAAGAAGTTGCGAGATTCATTGAGGAAACCCAGTGAGTTTCAGGTTGGGTGTCAGAAGCCGAAGACGGTGTTGTTGGACACGGGCGAGATGTTCGAACCTTATGCTTGGGCGCCAGCAATTCTTCCAATTCAAATTCTCAGGCTAGGAAAACTGATCATACTCTCAGTACCAGGAGAGTTCACAACAATGGCGGGGCGTCGACTAAGGGAAGCAGTAAAGGAAACTCTGATTAGCAATGGAAATGGAGAGTTTGATGATGATACCCACGTTGTGATTGCAGGGCTTACAAATGCATACTCCCAATATGTCGCTACTTTAGAAGAGTATGAACAACAACGATATGAAGCTGCCTCAACTCTCTATGGACCACACACTCTATCAGCATATATTCAAGAGTTCAAGAAACTAGCAATAGCAATAGCCAAAGGTGAAAAGATTGCCAAGCCAATCGTCTCACCGCCAGACCTTTCCTCCGTCCAACTCCGATTCGTATCAGACCCTTTCATAGAATCTCCACCGAACGGTGTCAACTTTGGAGACATTCAACAAGACATCAAATTACCAAAAGTTGGTTGGTTCAAAAGAGGGAGCAAACAGAAACCAACTGCCAGATTTTGGAGTGCAAACCCGAGATTCGATCTCTTGACCGAAGGGACCTATGCTGTGGTTGAAAGGCTAGAGAAACGGCGGTGGACACCGGTTTATGATGACGATGATTTCTCCTTGGTTTTTAAATGGGCATTAGACAACAACACATTCACCAAGAGCTTAGCGACTATCGAATGGGACATCCCAATAGACGCCAATCCAGGTGTGTATCGGTTACGACACTTCGGTTCATCAAAGAGTGCAATAAACTCAACCAACATATATTTCACTGGGGCTTCTAGTGCATTTGCAGTGTTCTAA

Coding sequence (CDS)

ATGCGGCGGCAGTTGTTGAAGGTTGTGGCCACTGCTGTGCTTGCAGCTGTGAGCTGCTGCTGTGCTGATGGGGATTGGTTGGTCGGCGTCGGGAGCTTCGACATGACTGGCCCGGCTGCCGAAGTGAACATGATGGGGTTCGGGAATCTATACAGAGAAGAAAACATAGCAATAAGCGGAATCCACACACACGCCGGCCCAGGCGGCTACTTACAATACCTAGTCTACTCCATAACATCACTTGGCTTCGTTCAACAATCCTTCGACGCGATCGTGAACGCAATCGAACAAAGCATCGTCCAAGCTCACGAAAGCCTAAAACCAGGGTTCATGTTAATCAACAAAGGGGAAGTGGAGAATGCGGGGATTAATAGAAGCCCAAGTGCTTACCTTATGAACCCACCGGAGGAACGAGCTCGATATCCAACCAATGTGGACACAGACATGAGCCTTGTGAAGGTGGTGGAGGGAGGCAGTGGTGTGAGCCTTGGCGCCTTCAGCTGGTTCCCCACGCATGGGACGTCGATGAGTAGAGATAATAAGCTCATTAGTGGGGATAACAAGGGCGCTGCTGCCAGGTTTTTTGAGGACTGGGTCGCCGCCGCCTCTAACCGTTCTACCAGAGCCACCTCCTCCAATAAGTCAGACATTGTGGAGTTGATGAAGAAAGCAGAGACGATAAAGGCGACCGGAGGGAAACGATGCAGCAAAACGAGTAGCCAATCATCAAAAGTGAGAAAGAACGACGGATCTCTGTTCGTCGGAGCATTTTGCCAATCCAACGTCGGCGATGTCACGCCCAATGTTCTTGGAGCCTTTTGCACCGATTCCGGCAAGCCCTGCGATTTCAATCGCTCCTCTTGCCATGGCAGTGATCTTCTATGCGTGGGTCGTGGGCCTGGGTTTCCAGATGAAATTCTAAGCACGAAGATCATTGGGGAGAGGCAGTTCTTGAAAGCGGTTGATTTGTTCACAACAGCAACGGAGAGGCTAACTGGGGAGATTGATTTTCGCCATGTGTATCTGAATTTCACGGATATTGAAGTGGAATTGGGTGGGGATAATGTTGTCAAGACATGTCCAGCTGCTCTTGGCCCTGGTTTTGCTGCCGGAACTACGGATGGCCCTGGTGTGTTTGGCTTTCAACAGGGCGATACTCAGATCAATAAGTTGTGGAAGAAGTTGCGAGATTCATTGAGGAAACCCAGTGAGTTTCAGGTTGGGTGTCAGAAGCCGAAGACGGTGTTGTTGGACACGGGCGAGATGTTCGAACCTTATGCTTGGGCGCCAGCAATTCTTCCAATTCAAATTCTCAGGCTAGGAAAACTGATCATACTCTCAGTACCAGGAGAGTTCACAACAATGGCGGGGCGTCGACTAAGGGAAGCAGTAAAGGAAACTCTGATTAGCAATGGAAATGGAGAGTTTGATGATGATACCCACGTTGTGATTGCAGGGCTTACAAATGCATACTCCCAATATGTCGCTACTTTAGAAGAGTATGAACAACAACGATATGAAGCTGCCTCAACTCTCTATGGACCACACACTCTATCAGCATATATTCAAGAGTTCAAGAAACTAGCAATAGCAATAGCCAAAGGTGAAAAGATTGCCAAGCCAATCGTCTCACCGCCAGACCTTTCCTCCGTCCAACTCCGATTCGTATCAGACCCTTTCATAGAATCTCCACCGAACGGTGTCAACTTTGGAGACATTCAACAAGACATCAAATTACCAAAAGTTGGTTGGTTCAAAAGAGGGAGCAAACAGAAACCAACTGCCAGATTTTGGAGTGCAAACCCGAGATTCGATCTCTTGACCGAAGGGACCTATGCTGTGGTTGAAAGGCTAGAGAAACGGCGGTGGACACCGGTTTATGATGACGATGATTTCTCCTTGGTTTTTAAATGGGCATTAGACAACAACACATTCACCAAGAGCTTAGCGACTATCGAATGGGACATCCCAATAGACGCCAATCCAGGTGTGTATCGGTTACGACACTTCGGTTCATCAAAGAGTGCAATAAACTCAACCAACATATATTTCACTGGGGCTTCTAGTGCATTTGCAGTGTTCTAA

Protein sequence

MRRQLLKVVATAVLAAVSCCCADGDWLVGVGSFDMTGPAAEVNMMGFGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESLKPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRSTRATSSNKSDIVELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAVF
BLAST of Cp4.1LG11g08010 vs. Swiss-Prot
Match: NCASE_ARATH (Neutral ceramidase OS=Arabidopsis thaliana GN=At2g38010 PE=3 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 3.8e-213
Identity = 383/662 (57.85%), Postives = 477/662 (72.05%), Query Frame = 1

Query: 47  FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESL 106
           +G LY E+N+AISGIHTHAGPGGYLQY+ Y +TSLGFV+QSFD +VN IEQSIVQAHESL
Sbjct: 102 YGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESL 161

Query: 107 KPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAF 166
           +PG   +NKG++ +AG+NRSPS+YL NP  ER++Y  +VD +M+LVK V+   G + G+F
Sbjct: 162 RPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMTLVKFVDSQLGPT-GSF 221

Query: 167 SWFPTHGTSMSRDNKLISGDNKGAAARFFEDWV------AAASNRSTRATSSNKSDIVE- 226
           +WF THGTSMSR N LISGDNKGAAARF EDW       + +S    R  S+  SD    
Sbjct: 222 NWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSSRNIPRRVSTIVSDFSRN 281

Query: 227 ---LMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTD 286
              L+  A T K++ G    K+    ++VR      FV AFCQSN GDV+PN LG FC D
Sbjct: 282 RDRLLDIAATYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCID 341

Query: 287 SGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEID 346
           +G PCDFN S+C+G + LC GRGPG+PDE  ST+IIGE+QF  AV+LF  ATE+L G+I 
Sbjct: 342 TGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIG 401

Query: 347 FRHVYLNFTDIEVEL----GGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLW 406
           ++H YL+F++++V +    GG   VKTCPAA+G GFAAGTTDGPG F F+QGD Q N  W
Sbjct: 402 YQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFW 461

Query: 407 KKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEF 466
           + +R+ LR P   QV CQKPK +LLDTGEM EPY WAP+ILPIQILR+G+L+ILSVPGEF
Sbjct: 462 RLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEF 521

Query: 467 TTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTL 526
           TTMAGRRLR+A+K  LIS+   EF ++ HVVIAGLTN YSQY+AT EEYE QRYE ASTL
Sbjct: 522 TTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTL 581

Query: 527 YGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGD 586
           YG HTL+AYIQEFKKLA A+  G  + +    PPDL   Q+  +S   ++S P GV FGD
Sbjct: 582 YGRHTLTAYIQEFKKLATALVNGLTLPRG-PQPPDLLDKQISLLSPVVVDSTPLGVKFGD 641

Query: 587 IQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERL-EKRRWTPVYDDDD 646
           ++ D+  P    F+RG  Q+  A FWS  PR DL+TEG++AVVE L E  +W PVYDDDD
Sbjct: 642 VKADV--PPKSTFRRG--QQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDD 701

Query: 647 FSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAF 694
           FSL FKW+      ++S ATIEW +P  A  GVYR+RH+G+SKS   S +  F+G+SSAF
Sbjct: 702 FSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASKSLFGSIS-SFSGSSSAF 756

BLAST of Cp4.1LG11g08010 vs. Swiss-Prot
Match: NCASE_ORYSJ (Neutral ceramidase OS=Oryza sativa subsp. japonica GN=Os01g0624000 PE=1 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 5.6e-209
Identity = 372/676 (55.03%), Postives = 485/676 (71.75%), Query Frame = 1

Query: 47  FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESL 106
           +G+LY E N+AISGIHTHAGPGGYLQY+VY +TSLGFV+QSFD IV+ IEQSIV+AH +L
Sbjct: 117 YGDLYNENNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVIVDGIEQSIVEAHNNL 176

Query: 107 KPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAF 166
           +PG + +NKG++ +AG+NRSPSAYL NP EER++Y  NVD +M+L+K V+   G  +G+F
Sbjct: 177 RPGKIFVNKGDLLDAGVNRSPSAYLNNPAEERSKYEYNVDKEMTLIKFVDDELG-PVGSF 236

Query: 167 SWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVA-------AASNRSTRATSSNKSDIV- 226
           +WF THGTSMSR N LISGDNKGAAARF EDW         +A   S    S +K+ ++ 
Sbjct: 237 NWFATHGTSMSRTNSLISGDNKGAAARFMEDWAEQMGLPKQSAHANSDDLRSLHKTSVLP 296

Query: 227 ---------------ELMKKAETIKATGGKRCSKTS-SQSSKVRKNDGSLFVGAFCQSNV 286
                          +L++ A + +A+GG+R + +S ++  +  + +   FV AFCQSN 
Sbjct: 297 RRVSTIIPEPNEITDDLIQLASSYEASGGRRLAGSSITRRIRSTQQNKPKFVSAFCQSNC 356

Query: 287 GDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVD 346
           GDV+PNVLG FC D+  PCDFN S+C+G + LC GRGPG+PDE  ST++IG RQFLKA D
Sbjct: 357 GDVSPNVLGTFCIDTNLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRVIGNRQFLKARD 416

Query: 347 LFTTATERLTGEIDFRHVYLNFTDIEVEL----GGDNVVKTCPAALGPGFAAGTTDGPGV 406
           LF +A+E + G+ID+RH YL+F+ +EV++    GG   VKTCPAA+G  FAAGTTDGPG 
Sbjct: 417 LFDSASEEIQGKIDYRHTYLDFSKLEVKVSTSAGGQQTVKTCPAAMGFAFAAGTTDGPGA 476

Query: 407 FGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQIL 466
           F F+QGD + N  WK +R+ L+ P + QV C  PK +LLDTGEM EPY WAPAILP+Q++
Sbjct: 477 FDFRQGDVKGNPFWKLVRNLLKTPGKDQVECHSPKPILLDTGEMKEPYDWAPAILPVQMI 536

Query: 467 RLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATL 526
           R+G+L+IL VPGEFTTMAGRRLR+AVK T++++GN EFD + HVV+AGLTN+YSQY+ T 
Sbjct: 537 RIGQLVILCVPGEFTTMAGRRLRDAVK-TVLTSGNSEFDKNIHVVLAGLTNSYSQYITTF 596

Query: 527 EEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSD 586
           EEY+ QRYE ASTLYGPHTLSAYIQEF+KLA+A+   +++      PPD+   Q+  +  
Sbjct: 597 EEYQIQRYEGASTLYGPHTLSAYIQEFQKLAMAMIANKEVPTNF-QPPDMLDKQIGLLPG 656

Query: 587 PFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERL 646
              +S P GV FGD+  D+  P    F +GS    T  F+SA PR DLLT+GT+A+VE+L
Sbjct: 657 VVFDSTPLGVKFGDVNSDV--PGNSTFNKGSTVNAT--FYSACPRNDLLTDGTFALVEKL 716

Query: 647 E-KRRWTPVYDDDDFSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAI 694
           +    W PVYDDDD+SL FKW+      ++S AT+EW +P DA  GVYRLRHFG+SK   
Sbjct: 717 DGNNNWVPVYDDDDWSLRFKWSRPARLSSRSFATLEWTVPEDAAAGVYRLRHFGASKPMF 776

BLAST of Cp4.1LG11g08010 vs. Swiss-Prot
Match: NCSEB_DICDI (Neutral ceramidase B OS=Dictyostelium discoideum GN=dcd2B PE=3 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 1.3e-128
Identity = 271/655 (41.37%), Postives = 379/655 (57.86%), Query Frame = 1

Query: 49  NLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESLKP 108
           N+Y E N+ +SG HTH+GP G+ QY +Y ITSLGF +++FD I N I Q+IV+AH+S++P
Sbjct: 125 NVYTEANVLLSGTHTHSGPAGFSQYALYGITSLGFYKKNFDTICNGIVQAIVKAHKSVQP 184

Query: 109 GFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAFSW 168
             M    GE+ N  INRSP AY  NP EE+A Y +NVD +M+++++ E  +G    A S+
Sbjct: 185 ANMFTETGELWNTNINRSPFAYDNNPEEEKAMYDSNVDKNMTVLRI-EDMNGNPFAAISF 244

Query: 169 FPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRSTRATSSNKSDIVELMKKAETIK 228
           F  H TSM+  N LISGDNKG A+                           L +K     
Sbjct: 245 FAVHCTSMNNTNHLISGDNKGYAS--------------------------YLWEKQVNGP 304

Query: 229 ATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSC 288
            T GK                   FV AF QSN GDV+PN  G  C D G PCD+  S+C
Sbjct: 305 GTAGK-----------------GPFVAAFGQSNEGDVSPNTRGPTCRD-GSPCDYKTSTC 364

Query: 289 HGSDLLCVGRGPGFPDEIL-STKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDI 348
           +G +  C   GPG   ++  ST+IIG  QF KA++LF  A+ +++G + +RH ++ FT++
Sbjct: 365 NGRNEECWSLGPGKDGDMFESTQIIGGNQFNKALELFNNASIQVSGPVQYRHSWVQFTNV 424

Query: 349 EVE----LGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGD--TQINKLWKKLRDSLRK 408
            VE     G DN   TC  A+G  FAAGTTDGPG F F Q D  T  N  W  + D + K
Sbjct: 425 SVEPPYNSGVDNAT-TCRGAMGYSFAAGTTDGPGAFNFVQSDNNTSGNPFWNFIGDFIAK 484

Query: 409 PSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLR 468
           P+  Q+ CQ PK +LLD G M EP  W P ++PIQI+ +G++++++VPGEFTTM+GRRLR
Sbjct: 485 PTPDQIRCQSPKPILLDVG-MVEPIPWVPDVMPIQIVTIGQIVLVAVPGEFTTMSGRRLR 544

Query: 469 EAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAY 528
            +V+E +     GE  ++  V+IAGL+N YS Y+AT EEY+ QRYE AST++GPHTL +Y
Sbjct: 545 NSVREII-----GESIENPIVLIAGLSNTYSGYIATFEEYQVQRYEGASTVFGPHTLGSY 604

Query: 529 IQEFKKLAIAIAKGEKI-AKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLP 588
           +QEF KLA +I  G  + A P  +P +L+   L F+    +++ P+  +FG++  D+ L 
Sbjct: 605 MQEFGKLAQSIVDGTTVPAGP--TPRNLTGHTLFFLPPVIVDAAPDFDDFGEVSIDVNL- 664

Query: 589 KVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLE-KRRWTPVYDDDDFSLVFKWA 648
                     +  +  F+  NPR D + E ++  V+ L    +WT V DD D+   FKW 
Sbjct: 665 -----NYSVNETVSCVFYGGNPRNDFMIESSFLSVDLLTGTDQWTTVLDDGDWDTKFKWK 717

Query: 649 LDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIY-FTGASSAFAV 694
           + +  F  SL TIEW I  D  PG YR+ H G +K    S+N+  + G S  F V
Sbjct: 725 MHDLGF--SLITIEWVIAPDTTPGTYRITHSGFAKKNPFSSNLTPYQGISRNFVV 717

BLAST of Cp4.1LG11g08010 vs. Swiss-Prot
Match: NCSEA_DICDI (Neutral ceramidase A OS=Dictyostelium discoideum GN=dcd2A PE=1 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 1.2e-115
Identity = 250/651 (38.40%), Postives = 363/651 (55.76%), Query Frame = 1

Query: 50  LYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESLKPG 109
           LY  +N+ +SG HTH+GP G+ +Y +Y IT+LGF +++FD I + I Q+IV+AH+S++P 
Sbjct: 120 LYTHDNVLLSGTHTHSGPAGFSEYALYGITALGFYKKNFDTICDGIVQAIVKAHKSVQPA 179

Query: 110 FMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAFSWF 169
            ML  +GE+ N+ INRSP AY  NP EE+A Y  NVD +M+++++ +             
Sbjct: 180 RMLTQQGELWNSNINRSPYAYDNNPEEEKAMYDANVDKNMTVIRIED------------- 239

Query: 170 PTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRSTRATSSNKSDIVELMKKAETIKA 229
                        +SG N  AA  FF     + +N +   +  NK     L +K     A
Sbjct: 240 -------------MSG-NPFAAISFFGVHCTSMNNTNHLISGDNKGYASYLWEK----HA 299

Query: 230 TGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCH 289
            G      T              F+ AF QSN GDV+PN  G  C D GKPCD+  S+C+
Sbjct: 300 NGQSSLPGTGP------------FIAAFGQSNEGDVSPNTRGPTCRD-GKPCDYKTSTCN 359

Query: 290 GSDLLCVGRGPGFPDEIL-STKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIE 349
           G    C   GPG   ++  ST+IIG  QF KA++LF  AT +++G+I +RH +  FT++ 
Sbjct: 360 GKVEECWALGPGTDGDMFESTQIIGGNQFNKALELFNNATIQVSGKIQYRHTWKPFTNVS 419

Query: 350 VELGGDNVVK---TCPAALGPGFAAGTTDGPGVFGFQQGDTQI-NKLWKKLRDSLRKPSE 409
           VE   ++ V+   TC  A+G  FA GTTDGPG F F QGD    N  W  +   + KP+ 
Sbjct: 420 VEAPYNSGVEGATTCRGAMGYSFAGGTTDGPGAFNFIQGDNSTTNPFWNFIGGIIAKPTP 479

Query: 410 FQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAV 469
            Q  CQ PK +L+D G M EP  W P ++P+QI+ LG++++++VPGEFTTM+GRRLR  V
Sbjct: 480 QQTACQAPKPILIDVG-MVEPIPWVPDVMPLQIITLGQIVLVAVPGEFTTMSGRRLRNTV 539

Query: 470 KETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQE 529
           +E +     G+  ++  V+IAGL N YS Y+AT EE++ QRYE AST++GPHTL AY QE
Sbjct: 540 REII-----GQSIENPIVLIAGLANTYSGYIATFEEFQVQRYEGASTVFGPHTLGAYQQE 599

Query: 530 FKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGW 589
           F  LA +I  G + A P   P ++S     F+    ++  P   +FGDI  D+       
Sbjct: 600 FANLAQSIVDGSQ-ADPGTFPRNMSGHTPFFLPPVIVDVAPKFDDFGDIYTDVSTTT--- 659

Query: 590 FKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKR-RWTPVYDDDDFSLVFKWALDNN 649
                 Q  T  F+ AN R + +TE ++  V++L+   +WT + +D D+     W + + 
Sbjct: 660 -PYSINQTVTVIFYGANLRNNFMTESSFLTVDQLQSNGQWTTILNDGDWDTKLYWKMHDL 713

Query: 650 TFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNI-YFTGASSAFAV 694
            F  SL T++W I     PG YR+ H G +K    S N+ ++ G SS F V
Sbjct: 720 GF--SLITVDWTISPITQPGTYRITHSGYAKKNPFSDNLTFYQGISSNFNV 713

BLAST of Cp4.1LG11g08010 vs. Swiss-Prot
Match: ASAH2_MOUSE (Neutral ceramidase OS=Mus musculus GN=Asah2 PE=1 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 1.2e-113
Identity = 259/663 (39.06%), Postives = 356/663 (53.70%), Query Frame = 1

Query: 47  FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESL 106
           +G+LYR +N+ +S IHTH+GP G+ QY +Y + S GF  ++F  IV+ I +SI  AH +L
Sbjct: 155 YGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNL 214

Query: 107 KPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAF 166
           KPG + INKG V N  INRSPS+YL+NP  ERARY +N D +M ++K+V+  +G  LG  
Sbjct: 215 KPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDL-NGEDLGLI 274

Query: 167 SWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRSTRATSSNKSDIVELMKKAET 226
           SWF  H  S                            N S    +S+       + + E 
Sbjct: 275 SWFAIHPVS---------------------------MNNSNHFVNSDNMGYAAYLFEQE- 334

Query: 227 IKATGGKRCSKTSSQSSKVRKNDGSL-----FVGAFCQSNVGDVTPNVLGAFCTDSGKPC 286
                               KN G L     FV  F  SN+GDV+PN+LG  C ++G+ C
Sbjct: 335 --------------------KNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESC 394

Query: 287 DFNRSSC-HGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHV 346
           D ++S+C +G   +C+  GPG  D   ST IIG   + KA +L+ +A++ +TG +   H 
Sbjct: 395 DNDKSTCPNGGPSMCMASGPG-QDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQ 454

Query: 347 YLNFTDIEVELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLR 406
           ++N TD+ V+L   + VKTC  ALG  FAAGT DG       QG T+ +  W  LRD L 
Sbjct: 455 WVNMTDVSVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLL 514

Query: 407 -KPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRR 466
            KPSE  V CQKPK +LL +GE+  P+ W P I+ +QI+ +G L I ++PGE TTM+GRR
Sbjct: 515 GKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRR 574

Query: 467 LREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLS 526
            REA+K+     G      D  VVIAGL+N Y+ Y+ T EEY+ QRYEAAST+YGPHTLS
Sbjct: 575 FREAIKKEFALYGM----KDMTVVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLS 634

Query: 527 AYIQEFKKLAIAIAKGEKIAKPIVSP-PDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIK 586
           AYIQ F+ LA AIA  + +A     P P      +  +     +  P G +FGD+ Q  K
Sbjct: 635 AYIQLFRDLAKAIAT-DTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKHFGDVLQPAK 694

Query: 587 LP-KVGWFKRGSKQKPTARFWSANPR--FDLLTEGTYAVVERLEK--RRWTPVYDDDDFS 646
              +VG       +     F  ANP+   +  T  T+  VE+ E     W  +Y+D  + 
Sbjct: 695 PEYRVG-------EVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWE 753

Query: 647 LVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKS---AINSTNIYFTGASSA 694
             F W         S ATI W IP  A PG+YR+R+FG ++       +  + F G SS 
Sbjct: 755 TRFYW--HKGILGLSNATIYWHIPDTAYPGIYRIRYFGHNRKQELLKPAVILAFEGISSP 753

BLAST of Cp4.1LG11g08010 vs. TrEMBL
Match: A0A0A0KII3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G361230 PE=4 SV=1)

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 571/661 (86.38%), Postives = 604/661 (91.38%), Query Frame = 1

Query: 44  MMGFGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAH 103
           M  FG++Y EEN+AISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSI+QAH
Sbjct: 1   MKTFGDVYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAH 60

Query: 104 ESLKPGFMLINKG----------EVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVK 163
           ESLKPGF+L NKG          EVENAGINRSPSAYLMNP EERA+Y  NVD +MS++K
Sbjct: 61  ESLKPGFILFNKGKQIRQLFHFWEVENAGINRSPSAYLMNPEEERAKYANNVDKEMSILK 120

Query: 164 VVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRSTRATSSN 223
            V+G +G S+GAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDW AA++ R+T   S+N
Sbjct: 121 FVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDW-AASNRRTTTTPSNN 180

Query: 224 KSDIVELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAF 283
            S I +LMKKA  IKATGGKRCSKTSS+SSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAF
Sbjct: 181 TSGIEDLMKKAGKIKATGGKRCSKTSSKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAF 240

Query: 284 CTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTG 343
           CTDSG PCDFN SSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKA DLFTTATE+LTG
Sbjct: 241 CTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTG 300

Query: 344 EIDFRHVYLNFTDIEVELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWK 403
           EIDFRHVYLNFTDIEV + G+NVV+TCPAALGPGFAAGTTDGPG FGFQQGDT+INKLWK
Sbjct: 301 EIDFRHVYLNFTDIEVAIDGNNVVRTCPAALGPGFAAGTTDGPGAFGFQQGDTEINKLWK 360

Query: 404 KLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFT 463
           +LRDSL+KPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLG LIILSVPGEFT
Sbjct: 361 QLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGNLIILSVPGEFT 420

Query: 464 TMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLY 523
           TMAGRRLREAVKETLISNGNG FDDDTHVVIAGLTN YSQYVAT EEYEQQRYEAASTLY
Sbjct: 421 TMAGRRLREAVKETLISNGNGVFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLY 480

Query: 524 GPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDI 583
           GPHTLSAYIQEFKKLAIAIAKGEKIAKPI SPPDLSSVQLR V DPF ESPPNGVNFGDI
Sbjct: 481 GPHTLSAYIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGESPPNGVNFGDI 540

Query: 584 QQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFS 643
           QQD++LPK GWFK GSKQKPTA FWSANPRFDLLTEGTYA+VERLEK+RWTP YDDDDFS
Sbjct: 541 QQDVELPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFS 600

Query: 644 LVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAV 695
           L FKW LD NTF  +L TIEWDIPIDA+PGVYRLRHFGSS+S INSTN+YFTGAS AFAV
Sbjct: 601 LFFKWKLD-NTFINNLVTIEWDIPIDADPGVYRLRHFGSSRSTINSTNVYFTGASRAFAV 659

BLAST of Cp4.1LG11g08010 vs. TrEMBL
Match: A0A067GZK8_CITSI (Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g047902mg PE=4 SV=1)

HSP 1 Score: 1003.0 bits (2592), Expect = 1.7e-289
Identity = 500/667 (74.96%), Postives = 566/667 (84.86%), Query Frame = 1

Query: 29  GVGSFDMTGPAAEVNMMGFGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSF 88
           G+ S  +T    E     FGNLY EENIAISGIHTHAGPGGYLQYL+YSITSLGFVQQSF
Sbjct: 62  GMASQLVTTKVLEKLKTRFGNLYTEENIAISGIHTHAGPGGYLQYLIYSITSLGFVQQSF 121

Query: 89  DAIVNAIEQSIVQAHESLKPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTD 148
           DAIV+AI QSIVQAH +LKPG + I KG+VENAGINRSPSAYL+NP EERARYP NVD +
Sbjct: 122 DAIVSAIVQSIVQAHNNLKPGSIFIRKGDVENAGINRSPSAYLLNPAEERARYPENVDKE 181

Query: 149 MSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRSTR 208
           M+L+K V+  SG S+GAF+W+ THGTSMSRDN LISGDNKGAAARFFEDW ++ +  S+ 
Sbjct: 182 MTLLKFVDAASGKSIGAFNWYATHGTSMSRDNLLISGDNKGAAARFFEDWFSSKNESSSI 241

Query: 209 ATSSNKSDIVELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPN 268
            T+  K     LM+K++TIKATGG++C KT+SQ  KVRKNDGSLFVGAFCQSNVGDVTPN
Sbjct: 242 TTNIGK-----LMEKSKTIKATGGQQCGKTTSQGFKVRKNDGSLFVGAFCQSNVGDVTPN 301

Query: 269 VLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTAT 328
           VLGAFC DSGKPCDFN SSCHG D LC+GRGPG+PDEILSTKIIGERQF KAVDLF +AT
Sbjct: 302 VLGAFCIDSGKPCDFNHSSCHGDDQLCIGRGPGYPDEILSTKIIGERQFQKAVDLFNSAT 361

Query: 329 ERLTGEIDFRHVYLNFTDIEVELGG--DNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDT 388
           ++L+G+ID+RHVY+NFT+IEV L G  +N VKTCPAALGPGFAAGTTDGPGVFGFQQGDT
Sbjct: 362 DQLSGKIDYRHVYINFTNIEVVLDGTGNNTVKTCPAALGPGFAAGTTDGPGVFGFQQGDT 421

Query: 389 QINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIIL 448
           +INKLWK+LRD L+KPS++Q  CQKPK VLL +GEMFEPYAWAPA+LPIQILRLGKLIIL
Sbjct: 422 EINKLWKQLRDVLKKPSQYQEECQKPKAVLLSSGEMFEPYAWAPAVLPIQILRLGKLIIL 481

Query: 449 SVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRY 508
           SVPGEFTTMAGRRLREAVK+TLI+NGNGEFD+DTH+VIAGLTNAYSQY+AT EEY  QRY
Sbjct: 482 SVPGEFTTMAGRRLREAVKDTLINNGNGEFDNDTHIVIAGLTNAYSQYIATFEEYAHQRY 541

Query: 509 EAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPN 568
           EAASTLYGPHTL AYIQEFKKLA A+AKGEK  K   SPPDLSSVQL    +P  +SPP 
Sbjct: 542 EAASTLYGPHTLPAYIQEFKKLAQAMAKGEKTTKG-PSPPDLSSVQLSLSLNPTGDSPPA 601

Query: 569 GVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPV 628
           G  FGDI++DI  PK G F +G   +P+A FWSANPR+DLLTEGTYAVVE L+ +RW PV
Sbjct: 602 GKMFGDIKEDINQPKDGSFNKG--DRPSATFWSANPRYDLLTEGTYAVVEMLQGKRWIPV 661

Query: 629 YDDDDFSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTG 688
           YDDDDFSL FKW+L+N +F   LATIEW++P  A PGVYRLRHFGSSK A+NSTN YFTG
Sbjct: 662 YDDDDFSLYFKWSLENTSF-HGLATIEWEVPTKAIPGVYRLRHFGSSKKAVNSTNHYFTG 719

Query: 689 ASSAFAV 694
           ASSAF V
Sbjct: 722 ASSAFTV 719

BLAST of Cp4.1LG11g08010 vs. TrEMBL
Match: V4T5S7_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10003183mg PE=4 SV=1)

HSP 1 Score: 1002.3 bits (2590), Expect = 2.9e-289
Identity = 496/649 (76.43%), Postives = 560/649 (86.29%), Query Frame = 1

Query: 47  FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESL 106
           FGNLY EENIAISGIHTHAGPGGYLQYL+YSITSLGFVQQSFDAIV+AI QSIVQAH +L
Sbjct: 194 FGNLYTEENIAISGIHTHAGPGGYLQYLIYSITSLGFVQQSFDAIVSAIVQSIVQAHNNL 253

Query: 107 KPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAF 166
           KPG + I KG+VENAGINRSPSAYL+NP EERARYP NVD +M+L+K V+  SG S+GAF
Sbjct: 254 KPGSIFIRKGDVENAGINRSPSAYLLNPAEERARYPENVDKEMTLLKFVDAASGKSIGAF 313

Query: 167 SWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRSTRATSSNKSDIVELMKKAET 226
           +W+ THGTSMSRDN LISGDNKGAAARFFEDW ++ +  S+  T+  K     LM+K++T
Sbjct: 314 NWYATHGTSMSRDNLLISGDNKGAAARFFEDWFSSKNESSSITTNIGK-----LMEKSKT 373

Query: 227 IKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRS 286
           IKATGG++C KT+SQ  KVRKNDGSLFVGAFCQSNVGDVTPNVLGAFC DSGKPCDFN S
Sbjct: 374 IKATGGQQCGKTTSQGFKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCIDSGKPCDFNHS 433

Query: 287 SCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTD 346
           SCHG D LC+GRGPG+PDEILSTKIIGERQF KAVDLF +AT++L+G+ID+RHVY+NFT+
Sbjct: 434 SCHGDDQLCIGRGPGYPDEILSTKIIGERQFQKAVDLFNSATDQLSGKIDYRHVYINFTN 493

Query: 347 IEVELGG--DNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSE 406
           IEV L G  +N VKTCPAALGPGFAAGTTDGPGVFGFQQGDT+INKLWK+LRD L+KPS+
Sbjct: 494 IEVGLDGTGNNTVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKLWKQLRDVLKKPSQ 553

Query: 407 FQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAV 466
           +Q  CQKPK VLL +GEMFEPYAWAPA+LPIQILRLGKLIILSVPGEFTTMAGRRLREAV
Sbjct: 554 YQEECQKPKAVLLSSGEMFEPYAWAPAVLPIQILRLGKLIILSVPGEFTTMAGRRLREAV 613

Query: 467 KETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQE 526
           K+TLI+NGNGEFD+DTH+VIAGLTNAYSQY+AT EEY  QRYEAASTLYGPHTL AYIQE
Sbjct: 614 KDTLINNGNGEFDNDTHIVIAGLTNAYSQYIATFEEYAHQRYEAASTLYGPHTLPAYIQE 673

Query: 527 FKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGW 586
           FKKLA A+AKGEK  K   SPPDLSSVQL    +P  +SPP G  FGDI++DI  PK G 
Sbjct: 674 FKKLAQAMAKGEKTTKG-PSPPDLSSVQLSLSLNPTGDSPPAGKMFGDIKEDINQPKDGS 733

Query: 587 FKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNT 646
           F +G   +P+A FWSANPR+DLLTEGTYAVVE L+ +RW PVYDDDDFSL FKW+L+N +
Sbjct: 734 FNKG--DRPSATFWSANPRYDLLTEGTYAVVEMLQGKRWIPVYDDDDFSLYFKWSLENTS 793

Query: 647 FTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAV 694
           F   LATIEW++P  A PGVYRLRHFGSSK A+NSTN YFTGASSAF V
Sbjct: 794 F-HGLATIEWEVPTKAIPGVYRLRHFGSSKKAVNSTNHYFTGASSAFTV 833

BLAST of Cp4.1LG11g08010 vs. TrEMBL
Match: B9R8J2_RICCO (Ceramidase, putative OS=Ricinus communis GN=RCOM_1600200 PE=4 SV=1)

HSP 1 Score: 995.0 bits (2571), Expect = 4.7e-287
Identity = 482/647 (74.50%), Postives = 556/647 (85.94%), Query Frame = 1

Query: 47  FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESL 106
           FGNLY +EN+AISGIHTHAGPGGYLQYL+Y +TSLGFVQQSFDAIVN I QSI+QAH++L
Sbjct: 108 FGNLYTKENLAISGIHTHAGPGGYLQYLIYHVTSLGFVQQSFDAIVNGIVQSIIQAHQNL 167

Query: 107 KPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAF 166
           KPG + IN G+VE AGINRSPSAYL+NP EERARYP NVD +M+L+K V+  +G+S+GAF
Sbjct: 168 KPGSIFINTGDVETAGINRSPSAYLLNPAEERARYPANVDKEMTLLKFVDSATGISIGAF 227

Query: 167 SWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRSTRATSSNKSDIVELMKKAET 226
           SW+ THGTSMSRDNKLISGDNKGAAARFFEDW    S    ++ SSN  DI +L++KA+ 
Sbjct: 228 SWYATHGTSMSRDNKLISGDNKGAAARFFEDWFT--STNKPKSASSNSVDIDKLIEKAQK 287

Query: 227 IKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRS 286
           I+ATGG+ C KT+SQ+ KVRKNDGS FVGAFCQSNVGDVTPNVLGAFC+DSGKPCDFN S
Sbjct: 288 IRATGGQPCGKTTSQAFKVRKNDGSRFVGAFCQSNVGDVTPNVLGAFCSDSGKPCDFNHS 347

Query: 287 SCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTD 346
           SCHG   LC+GRGPG+PDEILSTKIIGERQF KAVDLF +A+E+L+G+ID+RH+YLNFTD
Sbjct: 348 SCHGDVRLCLGRGPGYPDEILSTKIIGERQFDKAVDLFMSASEKLSGKIDYRHMYLNFTD 407

Query: 347 IEVELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQ 406
           +EV+LG     KTCPAALGPGFAAGTTDGPG+FGF+QGDT+IN+LW+++RD L+KPS++Q
Sbjct: 408 LEVDLGEQGKAKTCPAALGPGFAAGTTDGPGMFGFEQGDTEINELWRQVRDFLKKPSQYQ 467

Query: 407 VGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKE 466
             CQKPK VLL TGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKE
Sbjct: 468 EDCQKPKNVLLSTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKE 527

Query: 467 TLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQEFK 526
           TLISNG GEFDD+TH+VIAGLTN YSQYVAT+EEY QQRYEAASTLYGPHTLSAYIQEF 
Sbjct: 528 TLISNGGGEFDDETHIVIAGLTNTYSQYVATIEEYGQQRYEAASTLYGPHTLSAYIQEFN 587

Query: 527 KLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFK 586
           +LA A+AKGE++ K  + PPDLSS QLR + DP+I+SPP G+ FGD++QD+  PK G FK
Sbjct: 588 RLATAMAKGEQLPKIDLLPPDLSSKQLRLLLDPWIDSPPKGIKFGDMKQDVTQPKNGSFK 647

Query: 587 RGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFT 646
           +G   +PTA FWS NPRFDLLTEGT+AVVE L+  RW PVYDDDDFSL FKW LDN T +
Sbjct: 648 KG--DRPTATFWSGNPRFDLLTEGTFAVVEMLQGERWIPVYDDDDFSLYFKWKLDNKTLS 707

Query: 647 KSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAV 694
            SLA +EW++P +A  GVYRLRHFGSSK   NS   YFTGASSAF V
Sbjct: 708 -SLARVEWEVPNEAISGVYRLRHFGSSKKTNNSPVEYFTGASSAFTV 749

BLAST of Cp4.1LG11g08010 vs. TrEMBL
Match: A0A067KPB8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_08134 PE=4 SV=1)

HSP 1 Score: 985.7 bits (2547), Expect = 2.8e-284
Identity = 482/649 (74.27%), Postives = 557/649 (85.82%), Query Frame = 1

Query: 47  FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESL 106
           FGNLY EEN+AISGIHTHAGPGGYLQYL+Y +TSLGFVQQSFDAIVNAI QSIVQAHE+L
Sbjct: 112 FGNLYSEENLAISGIHTHAGPGGYLQYLLYHVTSLGFVQQSFDAIVNAIIQSIVQAHENL 171

Query: 107 KPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAF 166
           KPG + INKG+VENAGINRSPSAYL+NP EERARY +NVD +M+L+K V+G +G S+GAF
Sbjct: 172 KPGSIFINKGDVENAGINRSPSAYLLNPEEERARYSSNVDKEMTLLKFVDGETGKSIGAF 231

Query: 167 SWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRSTRATS--SNKSDIVELMKKA 226
           SW+ THGTSMSRDNKLISGDNKGAAARFFEDW A+ +  +T + +  S  SDI  L++KA
Sbjct: 232 SWYATHGTSMSRDNKLISGDNKGAAARFFEDWFASTTKPNTTSVTNFSTASDIDMLIEKA 291

Query: 227 ETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFN 286
           + IKATGG+ C KT+SQ  KVRKN+GSLFVGAFCQSNVGDVTPNVLGAFCTD+GKPCDFN
Sbjct: 292 QNIKATGGQTCGKTTSQVFKVRKNNGSLFVGAFCQSNVGDVTPNVLGAFCTDTGKPCDFN 351

Query: 287 RSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNF 346
            SSCHG   LC+GRGPG+PDEILSTKIIGERQF KAVDLF +A+E L G+ID+RH+YLNF
Sbjct: 352 HSSCHGDVRLCLGRGPGYPDEILSTKIIGERQFNKAVDLFMSASEELNGKIDYRHLYLNF 411

Query: 347 TDIEVELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSE 406
           TD+EV++ G   VKTCPAALGPGFAAGTTDGPG+FGF+QGDT IN+LW+K+RD LRKPS+
Sbjct: 412 TDLEVDIDGKGKVKTCPAALGPGFAAGTTDGPGMFGFEQGDTNINELWRKVRDLLRKPSK 471

Query: 407 FQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAV 466
           +Q  CQKPK VL+ TGEMFEPYAWAPAILPIQILR+GKLIILSVPGEFTTMAGRRLREAV
Sbjct: 472 YQEHCQKPKNVLISTGEMFEPYAWAPAILPIQILRVGKLIILSVPGEFTTMAGRRLREAV 531

Query: 467 KETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQE 526
           KETLI NG GEFD+DTH+VIAGLTN YSQYVAT EEY QQRYEAASTLYGPHTLSAYIQE
Sbjct: 532 KETLIRNG-GEFDNDTHIVIAGLTNTYSQYVATTEEYGQQRYEAASTLYGPHTLSAYIQE 591

Query: 527 FKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGW 586
           F +LA A+A+GE++ K  + PPDLSS QLR + DP+I++PP G+ FGD++QD+  PK G 
Sbjct: 592 FNRLAEAMARGEQLIKTDLVPPDLSSKQLRLLLDPWIDTPPKGIKFGDVKQDVSQPKEGS 651

Query: 587 FKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNT 646
           FK+G   +P+A FWS NPRFDLLTEGT+AVVE+L+  RW PVYDDDDFSL FKW LD+ T
Sbjct: 652 FKKG--DRPSATFWSGNPRFDLLTEGTFAVVEKLQGERWIPVYDDDDFSLYFKWRLDDGT 711

Query: 647 FTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAV 694
            + S+ATIEW++P +A  GVYRLRHFGS K   +S   YFTG SSAF V
Sbjct: 712 LS-SIATIEWEVPKEATSGVYRLRHFGSFKKTADSHIEYFTGTSSAFTV 756

BLAST of Cp4.1LG11g08010 vs. TAIR10
Match: AT2G38010.2 (AT2G38010.2 Neutral/alkaline non-lysosomal ceramidase)

HSP 1 Score: 729.2 bits (1881), Expect = 2.4e-210
Identity = 383/697 (54.95%), Postives = 477/697 (68.44%), Query Frame = 1

Query: 47  FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESL 106
           +G LY E+N+AISGIHTHAGPGGYLQY+ Y +TSLGFV+QSFD +VN IEQSIVQAHESL
Sbjct: 102 YGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESL 161

Query: 107 KPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAF 166
           +PG   +NKG++ +AG+NRSPS+YL NP  ER++Y  +VD +M+LVK V+   G + G+F
Sbjct: 162 RPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMTLVKFVDSQLGPT-GSF 221

Query: 167 SWFPTHGTSMSRDNKLISGDNKGAAARFFEDWV------AAASNRSTRATSSNKSDI--- 226
           +WF THGTSMSR N LISGDNKGAAARF EDW       + +S    R  S+  SD    
Sbjct: 222 NWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSSRNIPRRVSTIVSDFSRN 281

Query: 227 ------------------------------------VELMKKAETIKATGGKRCSKTSSQ 286
                                                 L+  A T K++ G    K+   
Sbjct: 282 RESYPFLELPNVAILYIAVAERLMWKVMVLFYFVSESRLLDIAATYKSSRGHSVDKSLDV 341

Query: 287 SSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPG 346
            ++VR      FV AFCQSN GDV+PN LG FC D+G PCDFN S+C+G + LC GRGPG
Sbjct: 342 KTRVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPG 401

Query: 347 FPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVEL----GGDNVV 406
           +PDE  ST+IIGE+QF  AV+LF  ATE+L G+I ++H YL+F++++V +    GG   V
Sbjct: 402 YPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETV 461

Query: 407 KTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLL 466
           KTCPAA+G GFAAGTTDGPG F F+QGD Q N  W+ +R+ LR P   QV CQKPK +LL
Sbjct: 462 KTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILL 521

Query: 467 DTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFD 526
           DTGEM EPY WAP+ILPIQILR+G+L+ILSVPGEFTTMAGRRLR+A+K  LIS+   EF 
Sbjct: 522 DTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFS 581

Query: 527 DDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEK 586
           ++ HVVIAGLTN YSQY+AT EEYE QRYE ASTLYG HTL+AYIQEFKKLA A+  G  
Sbjct: 582 NNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLT 641

Query: 587 IAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARF 646
           + +    PPDL   Q+  +S   ++S P GV FGD++ D+  P    F+RG  Q+  A F
Sbjct: 642 LPRG-PQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADV--PPKSTFRRG--QQVNATF 701

Query: 647 WSANPRFDLLTEGTYAVVERL-EKRRWTPVYDDDDFSLVFKWALDNNTFTKSLATIEWDI 694
           WS  PR DL+TEG++AVVE L E  +W PVYDDDDFSL FKW+      ++S ATIEW +
Sbjct: 702 WSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRV 761

BLAST of Cp4.1LG11g08010 vs. TAIR10
Match: AT1G07380.1 (AT1G07380.1 Neutral/alkaline non-lysosomal ceramidase)

HSP 1 Score: 708.8 bits (1828), Expect = 3.4e-204
Identity = 368/672 (54.76%), Postives = 477/672 (70.98%), Query Frame = 1

Query: 47  FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESL 106
           +G+LY E+N+ ISGIHTHAGPGGYLQY+VY +TSLGFV+QSFDA+V+ IE SI+QAHE+L
Sbjct: 113 YGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDALVDGIENSIIQAHENL 172

Query: 107 KPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAF 166
           +PG + +N GE+ +AG+NRSPSAYL NP +ER+++  NVD +M+L+K V+   G  +G+F
Sbjct: 173 RPGSIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYNVDKEMTLLKFVDDQWG-PVGSF 232

Query: 167 SWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAA-----------ASNRSTRATSS--- 226
           +WF THGTSMSR N LISGDNKGAA+RF EDW               S+   R  SS   
Sbjct: 233 NWFATHGTSMSRTNSLISGDNKGAASRFMEDWYEQNTAERSYSEEFISDEIPRRVSSLIE 292

Query: 227 -NKSDIVELMKKAETIKATGGKRCSKTSSQSSKVR----KNDGSLFVGAFCQSNVGDVTP 286
            ++    EL++ A   ++  GK  ++ SS + +VR    K D   FV AFCQ+N GDV+P
Sbjct: 293 NHQDSHHELLELASYFESQPGKPVTRISSSARRVRSALRKADKPGFVSAFCQTNCGDVSP 352

Query: 287 NVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTA 346
           NVLGAFC D+G PCDFN S+C G + +C GRGPG+PDE  ST+IIGERQF  A++LF  A
Sbjct: 353 NVLGAFCLDTGLPCDFNHSTCGGKNEMCYGRGPGYPDEFESTRIIGERQFKMALELFNKA 412

Query: 347 TERLTGEIDFRHVYLNFTDIEVEL----GGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQ 406
           +E+L G++D+RHVY++F+ + V L    G   VVKTCPAA+G  FAAGTTDGPG F F Q
Sbjct: 413 SEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPAAMGFAFAAGTTDGPGAFDFTQ 472

Query: 407 GDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKL 466
           GD + N  W+ +R+ L+ P + Q+ C  PK +LLDTGEM +PY WAP+IL +Q+LR+G+L
Sbjct: 473 GDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEMTKPYDWAPSILSLQVLRIGQL 532

Query: 467 IILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQ 526
            ILSVPGEFTTMAGRRLR AVK  L ++GN +   + HVVIAGL N YSQYV T EEY+ 
Sbjct: 533 FILSVPGEFTTMAGRRLRYAVKTQLKNSGNKDLSGEIHVVIAGLANGYSQYVTTFEEYQV 592

Query: 527 QRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIES 586
           QRYE ASTLYGPHTLS YIQEFKKL+ ++     + +P   PPDL   QL F++   +++
Sbjct: 593 QRYEGASTLYGPHTLSGYIQEFKKLSKSLVLDMPV-QPGPQPPDLLDKQLSFLTPVMMDT 652

Query: 587 PPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEK--R 646
            P+G +FGD+  D+  PK    KRG+ Q  T  F SA PR DLLTEGT+ +VERLE+  +
Sbjct: 653 TPSGDSFGDVISDV--PKNLSLKRGNGQ-VTVVFRSACPRNDLLTEGTFTLVERLEQKDK 712

Query: 647 RWTPVYDDDDFSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTN 694
            WTPVYDDDD  L FKW+      ++S AT+EW IP  A+PGVYR+ HFG++K    S +
Sbjct: 713 TWTPVYDDDDLCLRFKWSRHKKLSSRSQATVEWRIPESASPGVYRITHFGAAKKLFGSVH 772

BLAST of Cp4.1LG11g08010 vs. TAIR10
Match: AT5G58980.1 (AT5G58980.1 Neutral/alkaline non-lysosomal ceramidase)

HSP 1 Score: 676.8 bits (1745), Expect = 1.4e-194
Identity = 360/666 (54.05%), Postives = 459/666 (68.92%), Query Frame = 1

Query: 47  FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESL 106
           +G LY EEN+AISG HTHAGPGGYLQY++Y +TSLGFV QSF+A+V+ IEQSI+QAHE+L
Sbjct: 105 YGELYTEENVAISGTHTHAGPGGYLQYILYLVTSLGFVHQSFNALVDGIEQSIIQAHENL 164

Query: 107 KPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAF 166
           +PG +LINKGE+ +AG+NRSPSAYL NP  ER++Y  +VD +M+LVK V+          
Sbjct: 165 RPGSILINKGELLDAGVNRSPSAYLNNPAHERSKYEYDVDKEMTLVKFVD---------- 224

Query: 167 SWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASN------RSTRATSSNKSDIVE- 226
                              D  G  AR  EDW    +        S R  SS  SD  + 
Sbjct: 225 -------------------DQWGPVARIMEDWFERENGCRSVDVESPRRVSSIISDPYDQ 284

Query: 227 -LMKKAETIKATGGKRCSKTSSQSSKVRKN----DGSLFVGAFCQSNVGDVTPNVLGAFC 286
            LM+ A ++ +TGGK  ++ SS + +VR      D   FV AFCQ+N GDV+PNVLGAFC
Sbjct: 285 DLMEMASSLLSTGGKTVTRMSSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFC 344

Query: 287 TDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGE 346
            D+G PC+FN+S+C G +  C GRGPG+PDE  ST+IIGERQF KA DLFT A+E + G+
Sbjct: 345 IDTGLPCEFNQSTCGGKNEQCYGRGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGK 404

Query: 347 IDFRHVYLNFTDIEVELGGDN----VVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINK 406
           +D+RH Y++F+ +EV + G N    VVKTCPAA+G GFAAGTTDGPG F F+QGD Q N 
Sbjct: 405 VDYRHAYVDFSQLEVTINGQNGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNP 464

Query: 407 LWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPG 466
            W+ +R+ L+ P+E QV CQ+PK +LLDTGEM +PY WAP+ILP+QILR+G+L+IL VPG
Sbjct: 465 FWRLVRNLLKNPTEEQVRCQRPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPG 524

Query: 467 EFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAAS 526
           EFTTMAGRRLR+AVK  L    NG    +  VVIAGLTN+YSQY+AT EEY+ QRYE AS
Sbjct: 525 EFTTMAGRRLRDAVKTVLKEGSNGR---EFSVVIAGLTNSYSQYIATFEEYQVQRYEGAS 584

Query: 527 TLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNF 586
           TLYGPHTLS YIQEFKKLA  +   +    P   PPDL   Q+  ++    +  P G  F
Sbjct: 585 TLYGPHTLSGYIQEFKKLANDLLSAQ-TTDPGPQPPDLLHKQISLLTPVVADMTPIGTAF 644

Query: 587 GDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVER-LEKRR-WTPVYD 646
           GD+  D+  P++  F++G+      +F SANPR DL+TEGT+A+VER LE R  W PVYD
Sbjct: 645 GDVTSDV--PRLSKFRKGA-DIVRVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYD 704

Query: 647 DDDFSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGAS 695
           DDDF L FKW+      T+S ATIEW IP  A+PGVYR+ HFGS+K+ I+S + +F+G+S
Sbjct: 705 DDDFCLRFKWSRPFKLSTQSTATIEWRIPETASPGVYRITHFGSAKTPISSIH-HFSGSS 733

BLAST of Cp4.1LG11g08010 vs. NCBI nr
Match: gi|659129879|ref|XP_008464893.1| (PREDICTED: neutral ceramidase-like [Cucumis melo])

HSP 1 Score: 1171.4 bits (3029), Expect = 0.0e+00
Identity = 574/653 (87.90%), Postives = 610/653 (93.42%), Query Frame = 1

Query: 42  VNMMGFGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQ 101
           +++  FG+LY EEN+AISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSI+Q
Sbjct: 97  ISVQRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQ 156

Query: 102 AHESLKPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGV 161
           AHESLKPGF+L NKGEV+NAGINRSPSAYLMNP EERA+Y  NVD +MS++K V+G SG 
Sbjct: 157 AHESLKPGFILFNKGEVDNAGINRSPSAYLMNPEEERAKYANNVDKEMSILKFVDGESGN 216

Query: 162 SLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRSTRATSSNKSDIVELM 221
           S+GAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDW AA++ R+T   S+N S I ELM
Sbjct: 217 SIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDW-AASNGRTTTTPSNNASVIEELM 276

Query: 222 KKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPC 281
           KKAE IKATGGK+CSKTSS+SSKVRKNDGSLFV AFCQSNVGDVTPNVLGAFC DSGKPC
Sbjct: 277 KKAEKIKATGGKQCSKTSSKSSKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCIDSGKPC 336

Query: 282 DFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVY 341
           DFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKA DLFTTATE+LTGEIDFRHVY
Sbjct: 337 DFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVY 396

Query: 342 LNFTDIEVELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRK 401
           LNFTDIEVE+ G+NVV+TCPAALGPGFAAGTTDGPGVFGFQQGDT+INKLWK+LRDSL+K
Sbjct: 397 LNFTDIEVEIDGNNVVRTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKLWKQLRDSLKK 456

Query: 402 PSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLR 461
           PSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLR
Sbjct: 457 PSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLR 516

Query: 462 EAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAY 521
           EAVKETLISNGNGEFDDDTHVVIAGLTN YSQYVAT EEYEQQRYEAASTLYGPHTLSAY
Sbjct: 517 EAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAY 576

Query: 522 IQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPK 581
           IQEFKKLA AIAKGEK+AKPI SPPDLSSVQLR V DPF ESPP+GVNFGDIQQD++LPK
Sbjct: 577 IQEFKKLASAIAKGEKVAKPIASPPDLSSVQLRLVMDPFGESPPDGVNFGDIQQDVELPK 636

Query: 582 VGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALD 641
            GWFK GSKQKPTA FWSANPRFDLLTEGTYA+VERLEK+RWTP YDDDDFS+ FKW LD
Sbjct: 637 DGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSVSFKWKLD 696

Query: 642 NNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAVF 695
           N T   SLATIEWDIPIDA+PGVYRLRHFGSS+S INSTNIYFTGAS AFAVF
Sbjct: 697 NTTLINSLATIEWDIPIDADPGVYRLRHFGSSRSTINSTNIYFTGASRAFAVF 748

BLAST of Cp4.1LG11g08010 vs. NCBI nr
Match: gi|449453075|ref|XP_004144284.1| (PREDICTED: neutral ceramidase-like [Cucumis sativus])

HSP 1 Score: 1160.6 bits (3001), Expect = 0.0e+00
Identity = 570/648 (87.96%), Postives = 603/648 (93.06%), Query Frame = 1

Query: 47  FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESL 106
           FG++Y EEN+AISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSI+QAHESL
Sbjct: 93  FGDVYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESL 152

Query: 107 KPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAF 166
           KPGF+L NKGEVENAGINRSPSAYLMNP EERA+Y  NVD +MS++K V+G +G S+GAF
Sbjct: 153 KPGFILFNKGEVENAGINRSPSAYLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAF 212

Query: 167 SWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRSTRATSSNKSDIVELMKKAET 226
           SWFPTHGTSMSRDNKLISGDNKGAAARFFEDW AA++ R+T   S+N S I +LMKKA  
Sbjct: 213 SWFPTHGTSMSRDNKLISGDNKGAAARFFEDW-AASNRRTTTTPSNNTSGIEDLMKKAGK 272

Query: 227 IKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRS 286
           IKATGGKRCSKTSS+SSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSG PCDFN S
Sbjct: 273 IKATGGKRCSKTSSKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHS 332

Query: 287 SCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTD 346
           SCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKA DLFTTATE+LTGEIDFRHVYLNFTD
Sbjct: 333 SCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTD 392

Query: 347 IEVELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQ 406
           IEV + G+NVV+TCPAALGPGFAAGTTDGPG FGFQQGDT+INKLWK+LRDSL+KPSEFQ
Sbjct: 393 IEVAIDGNNVVRTCPAALGPGFAAGTTDGPGAFGFQQGDTEINKLWKQLRDSLKKPSEFQ 452

Query: 407 VGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKE 466
           VGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLG LIILSVPGEFTTMAGRRLREAVKE
Sbjct: 453 VGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGNLIILSVPGEFTTMAGRRLREAVKE 512

Query: 467 TLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQEFK 526
           TLISNGNG FDDDTHVVIAGLTN YSQYVAT EEYEQQRYEAASTLYGPHTLSAYIQEFK
Sbjct: 513 TLISNGNGVFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFK 572

Query: 527 KLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFK 586
           KLAIAIAKGEKIAKPI SPPDLSSVQLR V DPF ESPPNGVNFGDIQQD++LPK GWFK
Sbjct: 573 KLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGESPPNGVNFGDIQQDVELPKDGWFK 632

Query: 587 RGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFT 646
            GSKQKPTA FWSANPRFDLLTEGTYA+VERLEK+RWTP YDDDDFSL FKW LD NTF 
Sbjct: 633 TGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSLFFKWKLD-NTFI 692

Query: 647 KSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAVF 695
            +L TIEWDIPIDA+PGVYRLRHFGSS+S INSTN+YFTGAS AFAVF
Sbjct: 693 NNLVTIEWDIPIDADPGVYRLRHFGSSRSTINSTNVYFTGASRAFAVF 738

BLAST of Cp4.1LG11g08010 vs. NCBI nr
Match: gi|700192357|gb|KGN47561.1| (hypothetical protein Csa_6G361230 [Cucumis sativus])

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 571/661 (86.38%), Postives = 604/661 (91.38%), Query Frame = 1

Query: 44  MMGFGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAH 103
           M  FG++Y EEN+AISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSI+QAH
Sbjct: 1   MKTFGDVYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAH 60

Query: 104 ESLKPGFMLINKG----------EVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVK 163
           ESLKPGF+L NKG          EVENAGINRSPSAYLMNP EERA+Y  NVD +MS++K
Sbjct: 61  ESLKPGFILFNKGKQIRQLFHFWEVENAGINRSPSAYLMNPEEERAKYANNVDKEMSILK 120

Query: 164 VVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRSTRATSSN 223
            V+G +G S+GAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDW AA++ R+T   S+N
Sbjct: 121 FVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDW-AASNRRTTTTPSNN 180

Query: 224 KSDIVELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAF 283
            S I +LMKKA  IKATGGKRCSKTSS+SSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAF
Sbjct: 181 TSGIEDLMKKAGKIKATGGKRCSKTSSKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAF 240

Query: 284 CTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTG 343
           CTDSG PCDFN SSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKA DLFTTATE+LTG
Sbjct: 241 CTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTG 300

Query: 344 EIDFRHVYLNFTDIEVELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWK 403
           EIDFRHVYLNFTDIEV + G+NVV+TCPAALGPGFAAGTTDGPG FGFQQGDT+INKLWK
Sbjct: 301 EIDFRHVYLNFTDIEVAIDGNNVVRTCPAALGPGFAAGTTDGPGAFGFQQGDTEINKLWK 360

Query: 404 KLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFT 463
           +LRDSL+KPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLG LIILSVPGEFT
Sbjct: 361 QLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGNLIILSVPGEFT 420

Query: 464 TMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLY 523
           TMAGRRLREAVKETLISNGNG FDDDTHVVIAGLTN YSQYVAT EEYEQQRYEAASTLY
Sbjct: 421 TMAGRRLREAVKETLISNGNGVFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLY 480

Query: 524 GPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDI 583
           GPHTLSAYIQEFKKLAIAIAKGEKIAKPI SPPDLSSVQLR V DPF ESPPNGVNFGDI
Sbjct: 481 GPHTLSAYIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGESPPNGVNFGDI 540

Query: 584 QQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFS 643
           QQD++LPK GWFK GSKQKPTA FWSANPRFDLLTEGTYA+VERLEK+RWTP YDDDDFS
Sbjct: 541 QQDVELPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFS 600

Query: 644 LVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAV 695
           L FKW LD NTF  +L TIEWDIPIDA+PGVYRLRHFGSS+S INSTN+YFTGAS AFAV
Sbjct: 601 LFFKWKLD-NTFINNLVTIEWDIPIDADPGVYRLRHFGSSRSTINSTNVYFTGASRAFAV 659

BLAST of Cp4.1LG11g08010 vs. NCBI nr
Match: gi|568839895|ref|XP_006473911.1| (PREDICTED: neutral ceramidase-like [Citrus sinensis])

HSP 1 Score: 1010.7 bits (2612), Expect = 1.2e-291
Identity = 504/669 (75.34%), Postives = 569/669 (85.05%), Query Frame = 1

Query: 29  GVGSFDMTGPAAEVNMMGFGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSF 88
           G+ S  +T    E     FGNLY EENIAISGIHTHAGPGGYLQYL+YSITSLGFVQQSF
Sbjct: 91  GMASQLVTTKVLEKLKTRFGNLYTEENIAISGIHTHAGPGGYLQYLIYSITSLGFVQQSF 150

Query: 89  DAIVNAIEQSIVQAHESLKPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTD 148
           DAIV AI QSIVQAH +LKPG + I KG+VENAGINRSPSAYL+NP EERARYP NVD +
Sbjct: 151 DAIVTAIVQSIVQAHNNLKPGSIFIRKGDVENAGINRSPSAYLLNPAEERARYPENVDKE 210

Query: 149 MSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRSTR 208
           M+L+K V+  SG S+GAF+W+ THGTSMSRDN LISGDNKGAAARFFEDW ++ +  S+ 
Sbjct: 211 MTLLKFVDAASGKSIGAFNWYATHGTSMSRDNLLISGDNKGAAARFFEDWFSSKNESSST 270

Query: 209 ATS--SNKSDIVELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVT 268
            TS  S  SDI +LM+K++TIKATGG++C KT+SQ  KVRKNDGSLFVGAFCQSNVGDVT
Sbjct: 271 TTSKFSTSSDIGKLMEKSKTIKATGGQQCGKTTSQGFKVRKNDGSLFVGAFCQSNVGDVT 330

Query: 269 PNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTT 328
           PNVLGAFC DSGKPCDFN SSCHG D LC+GRGPG+PDEILSTKIIGERQF KAVDLF +
Sbjct: 331 PNVLGAFCIDSGKPCDFNHSSCHGDDQLCIGRGPGYPDEILSTKIIGERQFQKAVDLFNS 390

Query: 329 ATERLTGEIDFRHVYLNFTDIEVELGG--DNVVKTCPAALGPGFAAGTTDGPGVFGFQQG 388
           AT++L+G+ID+RHVY+NFT+IEV L G  +N VKTCPAALGPGFAAGTTDGPGVFGFQQG
Sbjct: 391 ATDQLSGKIDYRHVYINFTNIEVVLDGTGNNTVKTCPAALGPGFAAGTTDGPGVFGFQQG 450

Query: 389 DTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLI 448
           DT+INKLWK+LRD L+KPS++Q  CQKPK VLL +GEMFEPYAWAPA+LPIQILRLGKLI
Sbjct: 451 DTEINKLWKQLRDVLKKPSQYQEECQKPKAVLLSSGEMFEPYAWAPAVLPIQILRLGKLI 510

Query: 449 ILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQ 508
           ILSVPGEFTTMAGRRLREAVK+TLI+NGNGEFD+DTH+VIAGLTNAYSQY+AT EEY  Q
Sbjct: 511 ILSVPGEFTTMAGRRLREAVKDTLINNGNGEFDNDTHIVIAGLTNAYSQYIATFEEYAHQ 570

Query: 509 RYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESP 568
           RYEAASTLYGPHTL AYIQEFKKLA A+AKGEK  K   SPPDLSSVQL    +P  +SP
Sbjct: 571 RYEAASTLYGPHTLPAYIQEFKKLAQAMAKGEKTTKG-PSPPDLSSVQLSLSLNPTGDSP 630

Query: 569 PNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWT 628
           P G  FGDI++DI  PK G F +G   +P+A FWSANPR+DLLTEGTYAVVE L+ +RW 
Sbjct: 631 PAGKMFGDIKEDINQPKDGSFNKG--DRPSATFWSANPRYDLLTEGTYAVVEMLQGKRWI 690

Query: 629 PVYDDDDFSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYF 688
           PVYDDDDFSL FKW+L+N +F   LATIEW++P  A PGVYRLRHFGSSK A+NSTN YF
Sbjct: 691 PVYDDDDFSLYFKWSLENTSF-HGLATIEWEVPTKAIPGVYRLRHFGSSKKAVNSTNHYF 750

Query: 689 TGASSAFAV 694
           TGASSAF V
Sbjct: 751 TGASSAFTV 755

BLAST of Cp4.1LG11g08010 vs. NCBI nr
Match: gi|645229279|ref|XP_008221393.1| (PREDICTED: neutral ceramidase-like [Prunus mume])

HSP 1 Score: 1004.2 bits (2595), Expect = 1.1e-289
Identity = 501/655 (76.49%), Postives = 566/655 (86.41%), Query Frame = 1

Query: 47  FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESL 106
           FG+LY EEN+AISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAH +L
Sbjct: 101 FGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHHNL 160

Query: 107 KPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAF 166
           KPG + INKG+V NAGINRSPSAYL+NP EERARYP+NVDT M+L+K V+  S  S+GAF
Sbjct: 161 KPGSVFINKGDVVNAGINRSPSAYLLNPAEERARYPSNVDTLMTLLKFVDDASAKSVGAF 220

Query: 167 SWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRSTRATSSNKS-------DIVE 226
           SWF THGTSMS++N LISGDNKGAAARFFEDW +++S+ ST ++ S+ +        ++ 
Sbjct: 221 SWFATHGTSMSKNNLLISGDNKGAAARFFEDWFSSSSSSSTTSSDSSSTTPADSSASLLS 280

Query: 227 LMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGK 286
           L+KKA+++KATGGK C K SS+ SKVRKN+GSLFVGAFCQSNVGDVTPNVLGAFCTDSG+
Sbjct: 281 LIKKAQSMKATGGKLCGKRSSRESKVRKNNGSLFVGAFCQSNVGDVTPNVLGAFCTDSGR 340

Query: 287 PCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRH 346
           PCDFN SSCHG+DLLC+GRGPG+PDEILSTKIIGERQF KA  LFTTAT++LTG ID+RH
Sbjct: 341 PCDFNHSSCHGNDLLCLGRGPGYPDEILSTKIIGERQFQKAAHLFTTATQQLTGTIDYRH 400

Query: 347 VYLNFTDIEVEL-GGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDS 406
           VYLNFTDIEVEL  G+  VKTCPAALGPGFAAGTTDGPG FGFQQGDT+IN+LW+KLRD+
Sbjct: 401 VYLNFTDIEVELLDGNEKVKTCPAALGPGFAAGTTDGPGAFGFQQGDTKINELWRKLRDA 460

Query: 407 LRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGR 466
           L+KP+ +QV CQKPKTVLLDTGEMF PYAWAPAILPIQ+LRLGKLIILSVPGEFTTMAGR
Sbjct: 461 LKKPTPYQVDCQKPKTVLLDTGEMFVPYAWAPAILPIQMLRLGKLIILSVPGEFTTMAGR 520

Query: 467 RLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTL 526
           RLREAVKETLISN NGEFDD+THVVIAGLTN YSQY+AT EEY QQRYEAASTLYGPHTL
Sbjct: 521 RLREAVKETLISNSNGEFDDETHVVIAGLTNTYSQYIATFEEYAQQRYEAASTLYGPHTL 580

Query: 527 SAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIK 586
           SAYIQEF KLA A+AKGEKI K   SPPDLSSVQLR + +P  + PP   NFGD++QDI 
Sbjct: 581 SAYIQEFDKLAKAMAKGEKITKG-PSPPDLSSVQLRLLLEPTGDLPPPHTNFGDMKQDII 640

Query: 587 LPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKW 646
           LPK G FK+G   +P A FWSANPR+DLLTEGT+AVVE L+ + W PVYDDDDF L FKW
Sbjct: 641 LPKGGSFKKG--DRPIATFWSANPRYDLLTEGTFAVVELLQGKHWVPVYDDDDFCLFFKW 700

Query: 647 ALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAV 694
            +DN+T +   ATIEW+IP DA+ GVYRLRHFGSSK   +S NIYFTGASS FAV
Sbjct: 701 KVDNST-SYGTATIEWEIPRDADSGVYRLRHFGSSKKTKDSPNIYFTGASSGFAV 751

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NCASE_ARATH3.8e-21357.85Neutral ceramidase OS=Arabidopsis thaliana GN=At2g38010 PE=3 SV=1[more]
NCASE_ORYSJ5.6e-20955.03Neutral ceramidase OS=Oryza sativa subsp. japonica GN=Os01g0624000 PE=1 SV=1[more]
NCSEB_DICDI1.3e-12841.37Neutral ceramidase B OS=Dictyostelium discoideum GN=dcd2B PE=3 SV=1[more]
NCSEA_DICDI1.2e-11538.40Neutral ceramidase A OS=Dictyostelium discoideum GN=dcd2A PE=1 SV=1[more]
ASAH2_MOUSE1.2e-11339.06Neutral ceramidase OS=Mus musculus GN=Asah2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KII3_CUCSA0.0e+0086.38Uncharacterized protein OS=Cucumis sativus GN=Csa_6G361230 PE=4 SV=1[more]
A0A067GZK8_CITSI1.7e-28974.96Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g047902mg PE=4 S... [more]
V4T5S7_9ROSI2.9e-28976.43Uncharacterized protein OS=Citrus clementina GN=CICLE_v10003183mg PE=4 SV=1[more]
B9R8J2_RICCO4.7e-28774.50Ceramidase, putative OS=Ricinus communis GN=RCOM_1600200 PE=4 SV=1[more]
A0A067KPB8_JATCU2.8e-28474.27Uncharacterized protein OS=Jatropha curcas GN=JCGZ_08134 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G38010.22.4e-21054.95 Neutral/alkaline non-lysosomal ceramidase[more]
AT1G07380.13.4e-20454.76 Neutral/alkaline non-lysosomal ceramidase[more]
AT5G58980.11.4e-19454.05 Neutral/alkaline non-lysosomal ceramidase[more]
Match NameE-valueIdentityDescription
gi|659129879|ref|XP_008464893.1|0.0e+0087.90PREDICTED: neutral ceramidase-like [Cucumis melo][more]
gi|449453075|ref|XP_004144284.1|0.0e+0087.96PREDICTED: neutral ceramidase-like [Cucumis sativus][more]
gi|700192357|gb|KGN47561.1|0.0e+0086.38hypothetical protein Csa_6G361230 [Cucumis sativus][more]
gi|568839895|ref|XP_006473911.1|1.2e-29175.34PREDICTED: neutral ceramidase-like [Citrus sinensis][more]
gi|645229279|ref|XP_008221393.1|1.1e-28976.49PREDICTED: neutral ceramidase-like [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006823Ceramidase_alk
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0006687 glycosphingolipid metabolic process
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0017040 ceramidase activity
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG11g08010.1Cp4.1LG11g08010.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006823Neutral/alkaline nonlysosomal ceramidasePANTHERPTHR12670CERAMIDASEcoord: 22..694
score:
NoneNo IPR availableunknownCoilCoilcoord: 484..504
scor
NoneNo IPR availablePANTHERPTHR12670:SF6SUBFAMILY NOT NAMEDcoord: 22..694
score:
NoneNo IPR availablePROFILEPS51257PROKAR_LIPOPROTEINcoord: 1..19
score:

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG11g08010Watermelon (Charleston Gray)cpewcgB124