CmaCh04G014580 (gene) Cucurbita maxima (Rimu)

NameCmaCh04G014580
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionPectinesterase
LocationCma_Chr04 : 7415195 .. 7418522 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACTCAGCTCCCTTCATTTTCCTTTTCCTCCTCTCTCTCCGCCTCGCCGGAGCTTCCGATTCTCCTCCGCCGTCCGCCGTCCTCTGTAAATCCACTCTATATCCTAAGCTTTGCCGCTCCATTCTCTCCTCCATCCGATTCTCCCCCTCCGATCCATACGGCTACGGAAAATTCTCCGTCAGGAAGTGTCTCAAACAGGCGATCAAAATGTCGAGCGTTATCGGCGAGTATCTCGACCGAGGAGGCGATTCGAGGTTGAATCGGCAGGAGGTTGGCGCTCTGGAAGACTGCAGAGAGCTTTCCGATTCGAATGTGGAGTTTCTCCAGTCGATTGATGGCGTATTGGAGGCAGCGGAGGGCGTTGATGAGGAACTGATGGAGCGTATTGAGAGTATTTTGAGCGCGATCGTGACGAACGGCCAGACTTGTATCGACGGATTGGTGGAGTCGAGAAGTAGTTTGGGGAATGCGCTATCGGCACCGCTGTTGAGCGCGGGGGAGCTGTACAGTGTATCGTTAGGACTGGTTAAGAACTCGCTGTCGCGGCGGTGGAAGAAGCGGAGCCAGAAGGGAGACCGCGAGGGAGGGAATTTCCCGGCGGCTGCGCAGAGCAGAGAGCCATTGAACACGCTGATCAAGGTACGATTCGATATGGCGCCGTTGTTATTTGGTCTCTGTTTGTCTGTTCGTTCGGTTACACGGACCATTACATTTGCTTTATTCAATGCATCAAAAAATTAGGCGAAAATAAGTTTTTGTTTGGATGTGGCCACTAAAATATATATATATTTTAAAATTTAATCTAATAAATAATAATGTTATATTTTTTTCAAAGTAATAGTAAAATAATAAATCATTAACAAATAAGCTTCTTCTTTAAGAATTAATCTTACAAATAAAATTGTTATTAAAATGGGTAATCAACCTTGTAATTAATGCTTCAAAAGTGCATTAAATTCTCCCATAATGACATTATTACTGAGCAGAAATCACAATTGATGGTAGCATTAAGGGGAATTAGTGAGAGAAAAAACATTTAAGTATTCATAGTTATATAATTTTAAAACTATGCTTTAATTTAATTTCATTCACATTTAATTGATTTCTGATTTCTTTCCAATTACTTATGAATTACTTACATTGGAACATCATCTTTATACAAAGTGAGGAATTTAACTCTTGATGAAGAACCTGTCCTAACTATAGTTTAATATTTTAATTTTTACCAATATGATCAGTAAAAACAGTATATTAAATAAATAACAAAAATGTTTTTTAGTGGAAAAAAAGATTATATATATATATATTATATATATTATATTTGTTCATTAAATTTATGTTAAATACGTTGATAATTTTGTTGGTATCACGGGAGAGATTCTAGGATTCCCAAGCTCCACCAAATGCATGTTACAAATTCAGATGTGGATTCTCCTCATAATTTTTTAACTTGCCTTTTTTCTAATTGTTTTATTCCTATCATTTGCCTTTTATTATATATATATATATATATATATATATAGATGTATATGTATATATATGTATATGTACATGTATGTATATGTATATGTACTTTCATGTATATGTGTGTGCGTATACATGTATATGTATATATATGTATATGCATATGTACTTTCATGTATATGTGTGCGTGTATATATATATGTATGTATGTATGTATGTATATGTATATGTATATGAATATGAATATGGGCTGGCAGCCGGACCAGCTAAAAGCAACTCCAAACTCCGATGTCATAGGTTCAAATTATACAGAGCGTGGATTCGATTGTTGTAATCGAATGATTTCTTTAAACTCTGTCTAGTATAAACTAAGTAAAAAAATCTTGCAACAAGTCTTGATGTATACAAAACGCACTAGGTTTTTGTCTTTGGGTACTACGGACCCTCTGCCATCTATTGCAGCAGAGCCGTTTCATGAGCGGAGGCTAAGACGAGAAAGAAGAGGCTTTCCCGTGGACTAGCGTGGGCTAAGCTGGGAGAGTTGATATATATATCAAAACTAGTCTTGGAATTTAAGACAACTAAGGGGCAATTGTTCCTTTTTCTTACCTGATCTGCTCAAGTTCTTTTTAGTATAAATTAGTTGCTGATTTTTGGAGGAGATTGCATTTTTTCCCTATTAAATTAACAATGATCTTAAGAGTTTGAATGAACAGGGACTACATTCAATGGGTTCGTGCAACCAATCCACAAAGTGTTCGGGAAGGAAGCGCATGTTGACTGATTTGGGCACCACAGGAATTTTGATCAACAACACTGTGGTTGTTAGCTCAACCGGTGCTGACAACTTCACATCAATTGGAGATGCAATTTCATTTGCTCCCAACAATTCAATGCCTCAAGATGGCTACTTTGTTATCTATGTTAGACAAGGATACTACGACGAATATGTTGTCGTCCCCAAGTACAAAACCAATATAATGCTCATCGGAGATGGCATTAACCGAACCGTCATCACGGGGAACCATAACGTTGTTGATGGTTGGACGACTTACAATTCCTCTACCTTTAGTAAGCAATTCACAAATCAGTTTAAAATTTATAATATTGTCGTATCAAAATTATTCCGAAATTGGTAGCTGAATGTTTGGGATATATGTTGGCAGCTGTTTATGGGGATGGATTTGTTGCAATAGACATGACATTCAGGAACACAGCAGGAGCAGAGAAGCACCAAGCAGTGGCAGTGAGGAACAGTGCAGATCTCTCAACATTCTACCGCTGTAGCTTTGAGGGATATCAAGACACTCTTTATGTACATTCCTTAAGGCAATTCTACAGGGAATGCGACGTTTATGGCACCGTCGACTTCATTTTTGGCAACGCTGCTGCAGTTTTCCAGCAATGCAATTTATACGCTAGAAAGCCACTCCCCAACCAAAAGAACGCCTTCACTGCACAGGGCCGAACCGATCCGAACCAAAGCACAGGCATCTCAATCCATAACTGCACCATCACGGCTGCCCCAAACTGGGTGATGGATTTGAATGAGACGACGACGAACTATCTCGGGCGGCCATGGAAGCCGTTCTCAAGGACGGTGTATATGCAGTCTTACATTGGGGATTTGATCAGCCCAGTGGGGTGGCTGGAGTGGAATGGAACGGTGGGATTGGACACATTGTATTATGGGGAGTTTGAGAATTATGGACCAGGAGCTAACACGAGCTTGAGGGTCGATTGGGATGGCCATAGCTTGATGAATATCACAGAGGCTATGAATTTTACTGTCTATAACTTCACTATGGGAGATACCTGGCTGCCCTATACAGATATCCCCTTTTCTGGAGGTTTGCTATGA

mRNA sequence

ATGAACTCAGCTCCCTTCATTTTCCTTTTCCTCCTCTCTCTCCGCCTCGCCGGAGCTTCCGATTCTCCTCCGCCGTCCGCCGTCCTCTGTAAATCCACTCTATATCCTAAGCTTTGCCGCTCCATTCTCTCCTCCATCCGATTCTCCCCCTCCGATCCATACGGCTACGGAAAATTCTCCGTCAGGAAGTGTCTCAAACAGGCGATCAAAATGTCGAGCGTTATCGGCGAGTATCTCGACCGAGGAGGCGATTCGAGGTTGAATCGGCAGGAGGTTGGCGCTCTGGAAGACTGCAGAGAGCTTTCCGATTCGAATGTGGAGTTTCTCCAGTCGATTGATGGCGTATTGGAGGCAGCGGAGGGCGTTGATGAGGAACTGATGGAGCGTATTGAGAGTATTTTGAGCGCGATCGTGACGAACGGCCAGACTTGTATCGACGGATTGGTGGAGTCGAGAAGTAGTTTGGGGAATGCGCTATCGGCACCGCTGTTGAGCGCGGGGGAGCTGTACAGTGTATCGTTAGGACTGGTTAAGAACTCGCTGTCGCGGCGGTGGAAGAAGCGGAGCCAGAAGGGAGACCGCGAGGGAGGGAATTTCCCGGCGGCTGCGCAGAGCAGAGAGCCATTGAACACGCTGATCAAGGGACTACATTCAATGGGTTCGTGCAACCAATCCACAAAGTGTTCGGGAAGGAAGCGCATGTTGACTGATTTGGGCACCACAGGAATTTTGATCAACAACACTGTGGTTGTTAGCTCAACCGGTGCTGACAACTTCACATCAATTGGAGATGCAATTTCATTTGCTCCCAACAATTCAATGCCTCAAGATGGCTACTTTGTTATCTATGTTAGACAAGGATACTACGACGAATATGTTGTCGTCCCCAAGTACAAAACCAATATAATGCTCATCGGAGATGGCATTAACCGAACCGTCATCACGGGGAACCATAACGTTGTTGATGGTTGGACGACTTACAATTCCTCTACCTTTACTGTTTATGGGGATGGATTTGTTGCAATAGACATGACATTCAGGAACACAGCAGGAGCAGAGAAGCACCAAGCAGTGGCAGTGAGGAACAGTGCAGATCTCTCAACATTCTACCGCTGTAGCTTTGAGGGATATCAAGACACTCTTTATGTACATTCCTTAAGGCAATTCTACAGGGAATGCGACGTTTATGGCACCGTCGACTTCATTTTTGGCAACGCTGCTGCAGTTTTCCAGCAATGCAATTTATACGCTAGAAAGCCACTCCCCAACCAAAAGAACGCCTTCACTGCACAGGGCCGAACCGATCCGAACCAAAGCACAGGCATCTCAATCCATAACTGCACCATCACGGCTGCCCCAAACTGGGTGATGGATTTGAATGAGACGACGACGAACTATCTCGGGCGGCCATGGAAGCCGTTCTCAAGGACGGTGTATATGCAGTCTTACATTGGGGATTTGATCAGCCCAGTGGGGTGGCTGGAGTGGAATGGAACGGTGGGATTGGACACATTGTATTATGGGGAGTTTGAGAATTATGGACCAGGAGCTAACACGAGCTTGAGGGTCGATTGGGATGGCCATAGCTTGATGAATATCACAGAGGCTATGAATTTTACTGTCTATAACTTCACTATGGGAGATACCTGGCTGCCCTATACAGATATCCCCTTTTCTGGAGGTTTGCTATGA

Coding sequence (CDS)

ATGAACTCAGCTCCCTTCATTTTCCTTTTCCTCCTCTCTCTCCGCCTCGCCGGAGCTTCCGATTCTCCTCCGCCGTCCGCCGTCCTCTGTAAATCCACTCTATATCCTAAGCTTTGCCGCTCCATTCTCTCCTCCATCCGATTCTCCCCCTCCGATCCATACGGCTACGGAAAATTCTCCGTCAGGAAGTGTCTCAAACAGGCGATCAAAATGTCGAGCGTTATCGGCGAGTATCTCGACCGAGGAGGCGATTCGAGGTTGAATCGGCAGGAGGTTGGCGCTCTGGAAGACTGCAGAGAGCTTTCCGATTCGAATGTGGAGTTTCTCCAGTCGATTGATGGCGTATTGGAGGCAGCGGAGGGCGTTGATGAGGAACTGATGGAGCGTATTGAGAGTATTTTGAGCGCGATCGTGACGAACGGCCAGACTTGTATCGACGGATTGGTGGAGTCGAGAAGTAGTTTGGGGAATGCGCTATCGGCACCGCTGTTGAGCGCGGGGGAGCTGTACAGTGTATCGTTAGGACTGGTTAAGAACTCGCTGTCGCGGCGGTGGAAGAAGCGGAGCCAGAAGGGAGACCGCGAGGGAGGGAATTTCCCGGCGGCTGCGCAGAGCAGAGAGCCATTGAACACGCTGATCAAGGGACTACATTCAATGGGTTCGTGCAACCAATCCACAAAGTGTTCGGGAAGGAAGCGCATGTTGACTGATTTGGGCACCACAGGAATTTTGATCAACAACACTGTGGTTGTTAGCTCAACCGGTGCTGACAACTTCACATCAATTGGAGATGCAATTTCATTTGCTCCCAACAATTCAATGCCTCAAGATGGCTACTTTGTTATCTATGTTAGACAAGGATACTACGACGAATATGTTGTCGTCCCCAAGTACAAAACCAATATAATGCTCATCGGAGATGGCATTAACCGAACCGTCATCACGGGGAACCATAACGTTGTTGATGGTTGGACGACTTACAATTCCTCTACCTTTACTGTTTATGGGGATGGATTTGTTGCAATAGACATGACATTCAGGAACACAGCAGGAGCAGAGAAGCACCAAGCAGTGGCAGTGAGGAACAGTGCAGATCTCTCAACATTCTACCGCTGTAGCTTTGAGGGATATCAAGACACTCTTTATGTACATTCCTTAAGGCAATTCTACAGGGAATGCGACGTTTATGGCACCGTCGACTTCATTTTTGGCAACGCTGCTGCAGTTTTCCAGCAATGCAATTTATACGCTAGAAAGCCACTCCCCAACCAAAAGAACGCCTTCACTGCACAGGGCCGAACCGATCCGAACCAAAGCACAGGCATCTCAATCCATAACTGCACCATCACGGCTGCCCCAAACTGGGTGATGGATTTGAATGAGACGACGACGAACTATCTCGGGCGGCCATGGAAGCCGTTCTCAAGGACGGTGTATATGCAGTCTTACATTGGGGATTTGATCAGCCCAGTGGGGTGGCTGGAGTGGAATGGAACGGTGGGATTGGACACATTGTATTATGGGGAGTTTGAGAATTATGGACCAGGAGCTAACACGAGCTTGAGGGTCGATTGGGATGGCCATAGCTTGATGAATATCACAGAGGCTATGAATTTTACTGTCTATAACTTCACTATGGGAGATACCTGGCTGCCCTATACAGATATCCCCTTTTCTGGAGGTTTGCTATGA

Protein sequence

MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNSLSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFTVYNFTMGDTWLPYTDIPFSGGLL
BLAST of CmaCh04G014580 vs. Swiss-Prot
Match: PME25_ARATH (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana GN=PME25 PE=2 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 1.1e-191
Identity = 329/550 (59.82%), Postives = 420/550 (76.36%), Query Frame = 1

Query: 20  SDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRKCLKQAIKMSSVIGEYL 79
           S S  PS + CKST YPKLCR+IL++++ SPSDPY YGKF++++CLKQA ++S VI  Y 
Sbjct: 74  SQSQSPS-LACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYA 133

Query: 80  DR--GGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELMERIESILSAI 139
            R          +E+GA+ DC ELS+ +V +L+++   L+ A+ +   L+E + S+LS +
Sbjct: 134 RRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVEHVNSLLSGV 193

Query: 140 VTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNSLSRRWKK-RSQKGDREG 199
           VTN QTC+DGLVE++S    A+ +P+ +   LYS+SLGLV ++L+R  K+ ++ KG   G
Sbjct: 194 VTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILG 253

Query: 200 GNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGTT---GILINNTVVVSS 259
           G     +  REPL TLIKGL    +C+    C    R L +LG T    IL++  V+V  
Sbjct: 254 G---GNSTYREPLETLIKGLRK--TCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGP 313

Query: 260 TGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKTNIMLIGDGINRTV 319
             +DNFT+I DAI+ APNN+ P+DGYFVIY R+G Y+EY+VVP  K N+ML+GDGIN+T+
Sbjct: 314 FKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTI 373

Query: 320 ITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCS 379
           ITGNHNVVDGWTTYN S+F V G+ F+A+D+TFRNTAG EKHQAVA+RN+A+ S+FYRCS
Sbjct: 374 ITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCS 433

Query: 380 FEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGR 439
           FEGYQDTLYVHSLRQFYRECD+YGTVDFIFGNAAA+FQ CN+YARKP+  QKNA TA GR
Sbjct: 434 FEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGR 493

Query: 440 TDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYMQSYIGDLISPVGW 499
            DPNQ+TGISI NCTI AAP+   +     T +LGRPWKP+SRTV+MQSYI D++ PVGW
Sbjct: 494 LDPNQNTGISIINCTIKAAPDLAAEPKSAMT-FLGRPWKPYSRTVFMQSYISDIVQPVGW 553

Query: 500 LEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFTVYNFTMGDTWLPY 559
           LEWNGT+GLDT+YYGE+ N+GPGANT+ RV W G++L+N+ EAMNFTVYNFTMGDTWLP 
Sbjct: 554 LEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQ 613

Query: 560 TDIPFSGGLL 564
           TDIPF GGLL
Sbjct: 614 TDIPFYGGLL 616

BLAST of CmaCh04G014580 vs. Swiss-Prot
Match: PME47_ARATH (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana GN=PME47 PE=2 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 2.8e-190
Identity = 331/549 (60.29%), Postives = 413/549 (75.23%), Query Frame = 1

Query: 22  SPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRKCLKQAIKMSSVIGEYLDR 81
           SP P  + CKST YPKLCR+ILS+++ SPSDPY YGKF++++CLKQA ++S VI  +  R
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 82  --GGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELMERIESILSAIVT 141
                     +EV A+ DC EL++ +VE+L+++   L+AAE +   L++R+ S+L  +VT
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAALVDRVTSLLGGVVT 199

Query: 142 NGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNSLSRRWKK-RSQKGDR-EGG 201
           N QTC+DGLV+++S    A+  PL +   LYSVSLGLV ++L+R  K+ +  KG    GG
Sbjct: 200 NQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSKGKIFGGG 259

Query: 202 NFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGTTG---ILINNTVVVSST 261
           N P     REPL TLIK L    +C++   C    R L +LG T    IL+   V V   
Sbjct: 260 NKPV----REPLETLIKVLRK--TCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPY 319

Query: 262 GADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKTNIMLIGDGINRTVI 321
             DNF +I +A++ APN++ P+ GYFVIY R G Y+EYVV+   K NIMLIGDGIN+T+I
Sbjct: 320 ETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTII 379

Query: 322 TGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCSF 381
           +GNH+ +DGWTTYNSSTF V GD FVA+D+TFRNTAG EKHQAVAVRN+AD STFYRCSF
Sbjct: 380 SGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSF 439

Query: 382 EGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRT 441
           EGYQDTLYVHSLRQFYRECD+YGT+DFIFGNAAA+FQ CN+YARKP+ NQKNA TA GRT
Sbjct: 440 EGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRT 499

Query: 442 DPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYMQSYIGDLISPVGWL 501
           DPNQ TGISI NCTI AAP+   D   T T +LGRPWKP+SRTVY+QSYI D++ PVGWL
Sbjct: 500 DPNQKTGISIINCTIGAAPDLAADPKSTMT-FLGRPWKPYSRTVYIQSYISDVVQPVGWL 559

Query: 502 EWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFTVYNFTMGDTWLPYT 561
           EWNGT GLDT+ YGE++N+GPGA+TS RV W G+SL+N+ +AMNFTVYNFT+GDTWLP T
Sbjct: 560 EWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQT 619

Query: 562 DIPFSGGLL 564
           DIPF GGLL
Sbjct: 620 DIPFYGGLL 621

BLAST of CmaCh04G014580 vs. Swiss-Prot
Match: PME41_ARATH (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana GN=PME41 PE=2 SV=2)

HSP 1 Score: 502.3 bits (1292), Expect = 7.0e-141
Identity = 273/570 (47.89%), Postives = 366/570 (64.21%), Query Frame = 1

Query: 7   IFLFLLSLRLAGASDSPP-PSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRKCL 66
           +FL L +L +A  +  P   S+ +CK+T  PK C+S+      S  D   YG+FS+RK L
Sbjct: 11  LFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQT--SQGDVREYGRFSLRKSL 70

Query: 67  KQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQS---IDGVLEAAEGV 126
            Q+ K +  I  YL R  ++ L++  VGAL+DCR L+    ++L +      +  +++ +
Sbjct: 71  TQSRKFTRTIDRYLKRN-NALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTL 130

Query: 127 DEELMERIESILSAIVTNGQTCIDGLVESRSS---LGNALSAPLLSAGELYSVSLGLVKN 186
                + I+++LSA +TN QTC+DG+  + SS   + N ++ PL++  +L+SVSL L   
Sbjct: 131 SFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLAL--- 190

Query: 187 SLSRRW--KKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQST----KCSGRKR 246
             ++ W  KK+ Q       +        +P      G   +     +       S RK 
Sbjct: 191 -FTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKL 250

Query: 247 MLTDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYV 306
              D     +L+++ V V+  G  NFT+I +A++ APN +    GYFVIYV  G Y+E V
Sbjct: 251 ADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENV 310

Query: 307 VVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAE 366
           V+ K K  +M+IGDGINRTV+TGN NVVDGWTT+NS+TF V    FVA++MTFRNTAG E
Sbjct: 311 VIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPE 370

Query: 367 KHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQC 426
           KHQAVA+R+SADLS FY CSFE YQDTLY HSLRQFYRECD+YGTVDFIFGNAA VFQ C
Sbjct: 371 KHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDC 430

Query: 427 NLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKP 486
           NLY R+P+ NQ NA TAQGRTDPNQ+TGISIHNCTI  A + V   N T   YLGRPWK 
Sbjct: 431 NLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSS-NYTVKTYLGRPWKE 490

Query: 487 FSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNI 546
           +SRTV+MQSYI +++ PVGW EWNG   L TLYY E+ N G G++T+ RV W G+ ++N 
Sbjct: 491 YSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINS 550

Query: 547 TEAMNFTVYNFTMGDTWLPYTDIPFSGGLL 564
           T+A NFTV NF +GD W+  + +P+  GLL
Sbjct: 551 TDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of CmaCh04G014580 vs. Swiss-Prot
Match: PME7_ARATH (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana GN=PME7 PE=2 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 1.2e-129
Identity = 265/587 (45.14%), Postives = 361/587 (61.50%), Query Frame = 1

Query: 4   APFIFLFLLSLRLAG---ASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 63
           +P   L  LS  L     +S +   S+ +CK+T  PK C+S+      S  +   YG FS
Sbjct: 3   SPIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPH---SQGNVQQYGCFS 62

Query: 64  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEV-GALEDCRELSDSNVEFL-QSIDGVLEA 123
           +RK L Q+ K    +  Y+ R  ++ L++  V  AL+DCR L+   +++L  S + V + 
Sbjct: 63  IRKSLSQSRKFIRTVDRYIKR--NAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDT 122

Query: 124 AEGVDEELM-----ERIESILSAIVTNGQTCIDGLVESRS-----SLGNALSAPLLSAGE 183
           +     + +     + I+++LSA +TN QTC++GL  + S     ++   ++ PL++  +
Sbjct: 123 SAKTSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTK 182

Query: 184 LYSVSLGLVKNSLSRRW--KKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQST 243
           L  VSL L     ++ W  KK+ + G         ++   +P      G   +    + T
Sbjct: 183 LLGVSLAL----FTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPL-KMTEKT 242

Query: 244 KC---SGRKRMLTDLGTTG-------ILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQ 303
           K    S  +R L D  + G       +LI++ V VS  G  NFT+I  A++ APNN+   
Sbjct: 243 KAVYESLSRRKLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGS 302

Query: 304 DGYFVIYVRQGYYDEYVVVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYG 363
            G+F+IYV  G Y+EY+ + K K  +M+IGDGIN+TV+TGN +VVDGWTT+NS+TF V  
Sbjct: 303 AGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTA 362

Query: 364 DGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVY 423
             FVA+++TFRNTAG EKHQAVA+R+ AD S FY CSFE YQDTLY HSLRQFYRECDVY
Sbjct: 363 PNFVAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVY 422

Query: 424 GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWV 483
           GTVDFIFGNAA VFQ CNLY RKP+PNQ NA TAQGR+DPNQ+TG SI NCTI  A + V
Sbjct: 423 GTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV 482

Query: 484 MDLNETTTNYLGRPWKPFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPG 543
              N T   YLGRPWK +SRTVYMQSYI   + PVGW EWNG   L TLYY E+ N GPG
Sbjct: 483 SS-NYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPG 542

Query: 544 ANTSLRVDWDGHSLMNITEAMNFTVYNFTMGDTWLPYTDIPFSGGLL 564
           +NT+ RV W G+ ++N T+A NFTV    +   W+  T +P++ GL+
Sbjct: 543 SNTTNRVTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of CmaCh04G014580 vs. Swiss-Prot
Match: PME20_ARATH (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana GN=PME20 PE=2 SV=2)

HSP 1 Score: 463.8 bits (1192), Expect = 2.7e-129
Identity = 252/558 (45.16%), Postives = 337/558 (60.39%), Query Frame = 1

Query: 12  LSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRKCLKQAIKM 71
           +S ++    ++  PS + C+    P  CRS+L +    P D Y YG+ S+R+ L +A + 
Sbjct: 22  ISAQIPAIGNATSPSNI-CRFAPDPSYCRSVLPN---QPGDIYSYGRLSLRRSLSRARRF 81

Query: 72  SSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELMERIE 131
            S+I   LDR G     +  VGALEDC+ L+   +++L S     ++ + +     E + 
Sbjct: 82  ISMIDAELDRKGKVAA-KSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDVH 141

Query: 132 SILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNSLSRRWKKRSQK 191
           + LSA +TN QTC++GL  + S   N LS  L +  +LY VSL L     S+ W  R Q+
Sbjct: 142 TFLSAAITNEQTCLEGLKSTASE--NGLSGDLFNDTKLYGVSLAL----FSKGWVPRRQR 201

Query: 192 G------DREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGTTGILI 251
                        F      + PL         M    ++   +  +R L       + +
Sbjct: 202 SRPIWQPQARFKKFFGFRNGKLPLK--------MTERARAVYNTVTRRKLLQSDADAVQV 261

Query: 252 NNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKTNIMLI 311
           ++ V V   G  NFT+I  AI+ APN +   +GYF+IYV  G Y+EYV VPK K  +M+I
Sbjct: 262 SDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMI 321

Query: 312 GDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSAD 371
           GDGIN+TVITGN +VVDGWTT+NS+TF + G  F+ +++T RNTAG  K QAVA+R+  D
Sbjct: 322 GDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGD 381

Query: 372 LSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQK 431
           LS FY CSFE YQDTLY HSLRQFYRECDVYGTVDFIFGNAA V Q CNLY R+P   Q 
Sbjct: 382 LSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQS 441

Query: 432 NAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYMQSYIG 491
           N  TAQGRTDPNQ+TG +IH CTI  A + +   N T   YLGRPWK +SRTV MQ+YI 
Sbjct: 442 NEVTAQGRTDPNQNTGTAIHGCTIRPADD-LATSNYTVKTYLGRPWKEYSRTVVMQTYID 501

Query: 492 DLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFTVYNFT 551
             + P GW  W+G   L TLYY E+ N GPG++T+ RV W G+ ++N T+A NFTV NF 
Sbjct: 502 GFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFL 559

Query: 552 MGDTWLPYTDIPFSGGLL 564
           +G+ W+  T +PF GGL+
Sbjct: 562 VGEGWIGQTGVPFVGGLI 559

BLAST of CmaCh04G014580 vs. TrEMBL
Match: A0A0A0KUN8_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G612810 PE=4 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 1.5e-288
Identity = 488/564 (86.52%), Postives = 526/564 (93.26%), Query Frame = 1

Query: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS PFIFL LLSL  AGAS SPPPS  LCKSTLYPKLCRSILS+IRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPPSTALCKSTLYPKLCRSILSTIRFSPSDPYGYGKFS 60

Query: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           V++C+KQA KMS+VIG+YL+RG DSRLNR E GAL DCR+LSD NVEFL+SI+ VLEAAE
Sbjct: 61  VKQCIKQATKMSTVIGDYLNRGRDSRLNRPEAGALSDCRDLSDLNVEFLRSIERVLEAAE 120

Query: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180
           GVDEEL+ER+ESILSAIVTNGQTCIDGLVESRSSLGNALS PLLSAGELYSVSLGLV N+
Sbjct: 121 GVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSGPLLSAGELYSVSLGLVSNA 180

Query: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCN-QSTKCSGRKRMLTDLG 240
           +SRRWKKR +KG   GG  P   +SREPL+TLIKGLH M  CN QSTKC GR+R+LTDLG
Sbjct: 181 MSRRWKKRREKGGGNGG-VPGGGRSREPLDTLIKGLHKMEPCNNQSTKCLGRQRLLTDLG 240

Query: 241 TTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYK 300
           +TGILINNTVVVSSTGADNFTSIGDAI+FAPNNSMPQDGYFVIYV++GYY+EYVVVPK+K
Sbjct: 241 STGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVKEGYYEEYVVVPKFK 300

Query: 301 TNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVA 360
           TNIMLIGDGINRT+ITGNHNVVDGWTTYNSSTFTV GDGFVAID+TFRNTAG EKHQAVA
Sbjct: 301 TNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 VRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARK 420
           +RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECD+YGTVDFIFGNAAAVFQQCNLYARK
Sbjct: 361 LRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARK 420

Query: 421 PLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVY 480
           PLPNQKNAFTAQGRTDPNQ+TGISIHNCTI AAP+WVMD N TTTNYLGRPWK +SRTVY
Sbjct: 421 PLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTTTNYLGRPWKQYSRTVY 480

Query: 481 MQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNF 540
           MQSYIGDLISPVGWLEWNGTVGL+TLYYGE+ENYGPGANTSLRV+W G SL+N+T+AMNF
Sbjct: 481 MQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLLNVTQAMNF 540

Query: 541 TVYNFTMGDTWLPYTDIPFSGGLL 564
           TVYNFTMGDTWLPYTDIPFSGGLL
Sbjct: 541 TVYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of CmaCh04G014580 vs. TrEMBL
Match: B9RPV5_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_1541960 PE=4 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 2.6e-219
Identity = 391/572 (68.36%), Postives = 461/572 (80.59%), Query Frame = 1

Query: 2   NSAPFIFLFLLSLRLAGASDSPPPS-----AVLCKSTLYPKLCRSILSSIRFSPSDPYGY 61
           N + F  L  LS  L  +S SPP S     ++ CKS+LYPKLCRSILS+ R SPSD Y Y
Sbjct: 3   NLSLFFALLFLSSALLISSQSPPQSQSQSPSLACKSSLYPKLCRSILSTYRSSPSDLYDY 62

Query: 62  GKFSVRKCLKQAIKMSSVIGEYLDRGGD-SRLNRQEVGALEDCRELSDSNVEFLQSIDGV 121
            KFSV++C+KQA ++S  I  YL      S++N +E+GALEDC EL+  NV++L +I   
Sbjct: 63  SKFSVKQCIKQANRLSKAINYYLTHDKHRSKINSKEIGALEDCHELTQLNVDYLGTISSE 122

Query: 122 LEAAEGVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLG 181
           L++AE +++EL+ER+ S+LS IVTN QTC DGLVES+SS+   L APL +   LYSVSLG
Sbjct: 123 LKSAESMNDELVERVTSLLSGIVTNQQTCYDGLVESKSSIVAVLQAPLTNVTRLYSVSLG 182

Query: 182 LVKNSLSRRWKK--RSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQS--TKCSGR 241
           LV ++L R  KK  R++KG   G       + REPLNTLIK L    SC+ S  ++C   
Sbjct: 183 LVTHALDRNLKKNKRNKKGSH-GKGILTKNRIREPLNTLIKALRK-SSCHTSGGSRC--- 242

Query: 242 KRMLTDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDE 301
           +R L D+   GILIN+TV+VS  G DNFTSIGDAI+ APNNS P+DGYFVIY R+GYY+E
Sbjct: 243 RRNLADMEEDGILINDTVIVSPYGTDNFTSIGDAIAIAPNNSKPEDGYFVIYAREGYYEE 302

Query: 302 YVVVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAG 361
           YV+VPKYK NI+LIGDGINRTVITGNH+VVDGWTT+NSST  V G+ FVA+D+TFRNTAG
Sbjct: 303 YVIVPKYKKNILLIGDGINRTVITGNHSVVDGWTTFNSSTVAVSGERFVAVDVTFRNTAG 362

Query: 362 AEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQ 421
            +KHQAVA+RN+ADLSTFYRCSFEGYQDTLYVHSLRQFYRECD+YGTVDFIFGN+AAVFQ
Sbjct: 363 PQKHQAVALRNNADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNSAAVFQ 422

Query: 422 QCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPW 481
            CNLYARKPLPNQKNAFTAQGRTDPNQ+TGISIHNCTI AAP+  MDLN +T N+LGRPW
Sbjct: 423 SCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIEAAPDLAMDLN-STLNFLGRPW 482

Query: 482 KPFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLM 541
           K +SRTV+MQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTS+RV W G++LM
Sbjct: 483 KQYSRTVFMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSMRVQWPGYNLM 542

Query: 542 NITEAMNFTVYNFTMGDTWLPYTDIPFSGGLL 564
           N+++A NFTVYNFTMGDTWLP TDIPFSGGLL
Sbjct: 543 NVSQAANFTVYNFTMGDTWLPETDIPFSGGLL 568

BLAST of CmaCh04G014580 vs. TrEMBL
Match: A0A067K1K9_JATCU (Pectinesterase OS=Jatropha curcas GN=JCGZ_18594 PE=4 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 2.8e-213
Identity = 378/569 (66.43%), Postives = 449/569 (78.91%), Query Frame = 1

Query: 1   MNSAPFIFLFL-LSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKF 60
           ++S+ FIF  L LS  L  +SD     ++ CKST YPKLCRSILS+ RFS SDPY YGKF
Sbjct: 4   LSSSFFIFTLLSLSSFLLVSSDQSQSPSIACKSTPYPKLCRSILSTFRFSSSDPYDYGKF 63

Query: 61  SVRKCLKQAIKMSSVIGEYLDRGG-DSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEA 120
           SV++CLKQ  ++S  I  YL      S+L+R+E+GAL DC EL+  +V++L++I   L+A
Sbjct: 64  SVKQCLKQTHRLSKAINYYLTHDKYRSKLSREEIGALTDCHELTQLSVDYLETISTELQA 123

Query: 121 AEGVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVK 180
           A  +  EL+ER+ S+LS +VTN  TC DGLV+S+SS+   L  P+ +  +LYSVSLGLV 
Sbjct: 124 AASLSGELVERVTSLLSGVVTNQDTCFDGLVDSKSSIAGGLYNPIANVSDLYSVSLGLVT 183

Query: 181 NSLSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLH-SMGSCNQSTKCSGRK---RM 240
           ++L R  KK   K  +   +  +    REPL++LIK LH +  SC++S     RK   R 
Sbjct: 184 HALDRNLKK--NKRSKNQRSVFSKKGVREPLDSLIKALHKTKSSCHKSGGSDCRKKVGRN 243

Query: 241 LTDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVV 300
           L D  + GILIN+TV+VS  G  NFTSIGDAI+ APNNS  +DGYFVIY R+GYY+EYVV
Sbjct: 244 LADTESGGILINDTVIVSPYGDYNFTSIGDAIAIAPNNSKQEDGYFVIYAREGYYEEYVV 303

Query: 301 VPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEK 360
           VPKYK NI+LIGDGINRTVITGNH+VVDGWTTYNSSTF V G+ F+A+D+TFRNTAG  K
Sbjct: 304 VPKYKKNILLIGDGINRTVITGNHSVVDGWTTYNSSTFAVSGERFIAVDITFRNTAGPAK 363

Query: 361 HQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCN 420
           HQAVAVRN+ADLSTFYRCSFEGYQDTLYVHSLRQFYRECD+YGTVDFIFGNAAAVFQ CN
Sbjct: 364 HQAVAVRNNADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQSCN 423

Query: 421 LYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPF 480
           LY RKPLPNQKNA TAQGRTDPNQ+TGISIHNCTI AAP+  M++N +T NYLGRPWK +
Sbjct: 424 LYTRKPLPNQKNAITAQGRTDPNQNTGISIHNCTIEAAPDLAMEVNSSTLNYLGRPWKEY 483

Query: 481 SRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNIT 540
           SRTVYM+SYIGDLI   GWL WNGTVGLDTLYYGEF+NYGPGANTSLRV W G+S+MN++
Sbjct: 484 SRTVYMESYIGDLIDKNGWLAWNGTVGLDTLYYGEFDNYGPGANTSLRVQWGGYSIMNVS 543

Query: 541 EAMNFTVYNFTMGDTWLPYTDIPFSGGLL 564
           EA+NFTVYNFTMGDTWLP TDIPF GGLL
Sbjct: 544 EAVNFTVYNFTMGDTWLPNTDIPFYGGLL 570

BLAST of CmaCh04G014580 vs. TrEMBL
Match: B9HVF5_POPTR (Pectinesterase OS=Populus trichocarpa GN=POPTR_0010s25400g PE=4 SV=2)

HSP 1 Score: 736.9 bits (1901), Expect = 1.9e-209
Identity = 371/567 (65.43%), Postives = 445/567 (78.48%), Query Frame = 1

Query: 6   FIFLFLLSLRLAGASDSPPPSAV--LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRK 65
           F FL + S  L  +   PPP +    CKSTLYPKLCRSIL++   S S+PY Y KFSV++
Sbjct: 12  FAFLSIFSPLLISSQSPPPPYSPSNACKSTLYPKLCRSILTTFP-SSSNPYEYSKFSVKQ 71

Query: 66  CLKQAIKMSSVIGEYLDRGGD-SRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGV 125
           CLKQA ++S VI  +L      S++  +E GAL+DC E  + NV++ ++I   L AAE +
Sbjct: 72  CLKQAKRLSKVIDYHLTHEKQLSKMTHEEFGALQDCHEFMELNVDYFETISSELVAAESM 131

Query: 126 DEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNSLS 185
            + L+ER+ S+LS +VTN QTC DGLV+S+SS+ +ALS PL +  +LYSVSL LV +SL 
Sbjct: 132 SDVLVERVTSLLSGVVTNQQTCYDGLVQSKSSIVSALSVPLSNVTQLYSVSLALVTHSLE 191

Query: 186 RRWKK-RSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTD-LGT 245
           +  KK + +KG  +G         REPL TLIK L    SC+++  C   +R+L+D  G 
Sbjct: 192 KNLKKNKRRKGSPQG---TVTRGVREPLETLIKALRKTSSCHETRNCHRGERILSDDAGD 251

Query: 246 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKT 305
            GIL+N+TV+V   G DNFT+IGDAI+FAPNNS P+DGYFVI+VR+G Y+EYVVVPK K 
Sbjct: 252 DGILVNDTVIVGPYGTDNFTTIGDAIAFAPNNSKPEDGYFVIFVREGIYEEYVVVPKNKK 311

Query: 306 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 365
           NI+LIG+GIN+TVITGNH+V+DGWTT+NSSTF V G+ FV IDMTFRNTAG EKHQAVA+
Sbjct: 312 NIVLIGEGINQTVITGNHSVIDGWTTFNSSTFAVSGERFVGIDMTFRNTAGPEKHQAVAL 371

Query: 366 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 425
           RN+ADLSTFYRCSFE YQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQ CNLYARKP
Sbjct: 372 RNNADLSTFYRCSFEAYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQNCNLYARKP 431

Query: 426 LPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNET----TTNYLGRPWKPFSR 485
           + NQKNAFTAQGRTDPNQ+TGISIHNCTI AAP+  MD N T    T N+LGRPWK +SR
Sbjct: 432 MLNQKNAFTAQGRTDPNQNTGISIHNCTIEAAPDLAMDRNSTDSNLTLNFLGRPWKEYSR 491

Query: 486 TVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEA 545
           TV MQSYIG+LI PVGWLEWNGTVGLDT+YYGEF+NYGPGANTS RV W G +LMN T+A
Sbjct: 492 TVIMQSYIGELIQPVGWLEWNGTVGLDTIYYGEFQNYGPGANTSRRVQWPGFNLMNATQA 551

Query: 546 MNFTVYNFTMGDTWLPYTDIPFSGGLL 564
           +NFTVYNFTMGDTWLPYTD+PFSGGL+
Sbjct: 552 VNFTVYNFTMGDTWLPYTDVPFSGGLV 574

BLAST of CmaCh04G014580 vs. TrEMBL
Match: D7TF25_VITVI (Pectinesterase OS=Vitis vinifera GN=VIT_13s0047g00230 PE=4 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 4.2e-209
Identity = 371/567 (65.43%), Postives = 448/567 (79.01%), Query Frame = 1

Query: 6   FIFLFLL---------SLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGY 65
           ++FLF+L         +L ++G +    PSA  CK+TLYPKLCRSILS+ R SP  P  Y
Sbjct: 4   YLFLFILFFTFLSFSPALLISGQAPPLTPSAA-CKATLYPKLCRSILSTFRSSPVRPDAY 63

Query: 66  GKFSVRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVL 125
           G+FSV++CLKQA +MS +IG YL       ++  E GAL+DCR+LS+ NV++LQ+I G L
Sbjct: 64  GQFSVKQCLKQARRMSELIGHYLTHNQRWPMSHAEAGALDDCRQLSELNVDYLQTISGEL 123

Query: 126 EAAEGVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGL 185
           ++AE + +EL+ER+ ++LS IVTN QTC DGLV+SR+S+  AL APL +A +LYSVSLGL
Sbjct: 124 KSAELMTDELVERVRTLLSGIVTNQQTCYDGLVDSRNSMVAALLAPLSNANQLYSVSLGL 183

Query: 186 VKNSLSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLT 245
           V  +LS+  K+R ++G  E        + REP + +I+ L   GS N S   S   R+L 
Sbjct: 184 VSRALSQTRKRRKRRGLTENRFLKELDRVREPTSKIIEVLKK-GSLNTSRGSSRGGRILA 243

Query: 246 DLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVP 305
           +L   G+L++NTV VS  G DNFT+I DAISFAPN+S  +DGYFVIYV++GYY+EY +VP
Sbjct: 244 ELVDGGVLVSNTVTVSPNGTDNFTTIADAISFAPNSSNIEDGYFVIYVKEGYYEEYPMVP 303

Query: 306 KYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQ 365
           KYK  IML+GDGINRTVITGN +VVDGWTT+NS+TF V G+ FVAID+TFRNTAG EKHQ
Sbjct: 304 KYKKGIMLLGDGINRTVITGNRSVVDGWTTFNSATFAVSGERFVAIDITFRNTAGPEKHQ 363

Query: 366 AVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLY 425
           AVAVRN+ADLSTFYRCSFEGYQDTLY HSLRQFYR+C VYGTVDFIFGN+A +FQ CNLY
Sbjct: 364 AVAVRNNADLSTFYRCSFEGYQDTLYAHSLRQFYRDCIVYGTVDFIFGNSACIFQNCNLY 423

Query: 426 ARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSR 485
           ARKP+PNQKNAFTAQGR DPNQ+TGISIHNCTI AAP+  MDLN +T NYLGRPWK +SR
Sbjct: 424 ARKPMPNQKNAFTAQGRMDPNQNTGISIHNCTIEAAPDLAMDLN-STLNYLGRPWKQYSR 483

Query: 486 TVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEA 545
           TVYMQSYIG LI PVGWLEWNGTVGLDTLYYGEFENYGPGANTS+RV W G++LMN ++A
Sbjct: 484 TVYMQSYIGSLIDPVGWLEWNGTVGLDTLYYGEFENYGPGANTSMRVQWPGYTLMNASQA 543

Query: 546 MNFTVYNFTMGDTWLPYTDIPFSGGLL 564
           +NFTVYNFTMGDTWL   DIPF GGLL
Sbjct: 544 VNFTVYNFTMGDTWLTNLDIPFYGGLL 567

BLAST of CmaCh04G014580 vs. TAIR10
Match: AT3G10720.2 (AT3G10720.2 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 671.0 bits (1730), Expect = 6.4e-193
Identity = 329/550 (59.82%), Postives = 420/550 (76.36%), Query Frame = 1

Query: 20  SDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRKCLKQAIKMSSVIGEYL 79
           S S  PS + CKST YPKLCR+IL++++ SPSDPY YGKF++++CLKQA ++S VI  Y 
Sbjct: 74  SQSQSPS-LACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYA 133

Query: 80  DR--GGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELMERIESILSAI 139
            R          +E+GA+ DC ELS+ +V +L+++   L+ A+ +   L+E + S+LS +
Sbjct: 134 RRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVEHVNSLLSGV 193

Query: 140 VTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNSLSRRWKK-RSQKGDREG 199
           VTN QTC+DGLVE++S    A+ +P+ +   LYS+SLGLV ++L+R  K+ ++ KG   G
Sbjct: 194 VTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILG 253

Query: 200 GNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGTT---GILINNTVVVSS 259
           G     +  REPL TLIKGL    +C+    C    R L +LG T    IL++  V+V  
Sbjct: 254 G---GNSTYREPLETLIKGLRK--TCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGP 313

Query: 260 TGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKTNIMLIGDGINRTV 319
             +DNFT+I DAI+ APNN+ P+DGYFVIY R+G Y+EY+VVP  K N+ML+GDGIN+T+
Sbjct: 314 FKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTI 373

Query: 320 ITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCS 379
           ITGNHNVVDGWTTYN S+F V G+ F+A+D+TFRNTAG EKHQAVA+RN+A+ S+FYRCS
Sbjct: 374 ITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCS 433

Query: 380 FEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGR 439
           FEGYQDTLYVHSLRQFYRECD+YGTVDFIFGNAAA+FQ CN+YARKP+  QKNA TA GR
Sbjct: 434 FEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGR 493

Query: 440 TDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYMQSYIGDLISPVGW 499
            DPNQ+TGISI NCTI AAP+   +     T +LGRPWKP+SRTV+MQSYI D++ PVGW
Sbjct: 494 LDPNQNTGISIINCTIKAAPDLAAEPKSAMT-FLGRPWKPYSRTVFMQSYISDIVQPVGW 553

Query: 500 LEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFTVYNFTMGDTWLPY 559
           LEWNGT+GLDT+YYGE+ N+GPGANT+ RV W G++L+N+ EAMNFTVYNFTMGDTWLP 
Sbjct: 554 LEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQ 613

Query: 560 TDIPFSGGLL 564
           TDIPF GGLL
Sbjct: 614 TDIPFYGGLL 616

BLAST of CmaCh04G014580 vs. TAIR10
Match: AT5G04970.1 (AT5G04970.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 666.4 bits (1718), Expect = 1.6e-191
Identity = 331/549 (60.29%), Postives = 413/549 (75.23%), Query Frame = 1

Query: 22  SPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRKCLKQAIKMSSVIGEYLDR 81
           SP P  + CKST YPKLCR+ILS+++ SPSDPY YGKF++++CLKQA ++S VI  +  R
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 82  --GGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELMERIESILSAIVT 141
                     +EV A+ DC EL++ +VE+L+++   L+AAE +   L++R+ S+L  +VT
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAALVDRVTSLLGGVVT 199

Query: 142 NGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNSLSRRWKK-RSQKGDR-EGG 201
           N QTC+DGLV+++S    A+  PL +   LYSVSLGLV ++L+R  K+ +  KG    GG
Sbjct: 200 NQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSKGKIFGGG 259

Query: 202 NFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGTTG---ILINNTVVVSST 261
           N P     REPL TLIK L    +C++   C    R L +LG T    IL+   V V   
Sbjct: 260 NKPV----REPLETLIKVLRK--TCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPY 319

Query: 262 GADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKTNIMLIGDGINRTVI 321
             DNF +I +A++ APN++ P+ GYFVIY R G Y+EYVV+   K NIMLIGDGIN+T+I
Sbjct: 320 ETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTII 379

Query: 322 TGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCSF 381
           +GNH+ +DGWTTYNSSTF V GD FVA+D+TFRNTAG EKHQAVAVRN+AD STFYRCSF
Sbjct: 380 SGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSF 439

Query: 382 EGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRT 441
           EGYQDTLYVHSLRQFYRECD+YGT+DFIFGNAAA+FQ CN+YARKP+ NQKNA TA GRT
Sbjct: 440 EGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRT 499

Query: 442 DPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYMQSYIGDLISPVGWL 501
           DPNQ TGISI NCTI AAP+   D   T T +LGRPWKP+SRTVY+QSYI D++ PVGWL
Sbjct: 500 DPNQKTGISIINCTIGAAPDLAADPKSTMT-FLGRPWKPYSRTVYIQSYISDVVQPVGWL 559

Query: 502 EWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFTVYNFTMGDTWLPYT 561
           EWNGT GLDT+ YGE++N+GPGA+TS RV W G+SL+N+ +AMNFTVYNFT+GDTWLP T
Sbjct: 560 EWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQT 619

Query: 562 DIPFSGGLL 564
           DIPF GGLL
Sbjct: 620 DIPFYGGLL 621

BLAST of CmaCh04G014580 vs. TAIR10
Match: AT4G02330.1 (AT4G02330.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 502.3 bits (1292), Expect = 3.9e-142
Identity = 273/570 (47.89%), Postives = 366/570 (64.21%), Query Frame = 1

Query: 7   IFLFLLSLRLAGASDSPP-PSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRKCL 66
           +FL L +L +A  +  P   S+ +CK+T  PK C+S+      S  D   YG+FS+RK L
Sbjct: 11  LFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQT--SQGDVREYGRFSLRKSL 70

Query: 67  KQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQS---IDGVLEAAEGV 126
            Q+ K +  I  YL R  ++ L++  VGAL+DCR L+    ++L +      +  +++ +
Sbjct: 71  TQSRKFTRTIDRYLKRN-NALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTL 130

Query: 127 DEELMERIESILSAIVTNGQTCIDGLVESRSS---LGNALSAPLLSAGELYSVSLGLVKN 186
                + I+++LSA +TN QTC+DG+  + SS   + N ++ PL++  +L+SVSL L   
Sbjct: 131 SFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLAL--- 190

Query: 187 SLSRRW--KKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQST----KCSGRKR 246
             ++ W  KK+ Q       +        +P      G   +     +       S RK 
Sbjct: 191 -FTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKL 250

Query: 247 MLTDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYV 306
              D     +L+++ V V+  G  NFT+I +A++ APN +    GYFVIYV  G Y+E V
Sbjct: 251 ADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENV 310

Query: 307 VVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAE 366
           V+ K K  +M+IGDGINRTV+TGN NVVDGWTT+NS+TF V    FVA++MTFRNTAG E
Sbjct: 311 VIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPE 370

Query: 367 KHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQC 426
           KHQAVA+R+SADLS FY CSFE YQDTLY HSLRQFYRECD+YGTVDFIFGNAA VFQ C
Sbjct: 371 KHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDC 430

Query: 427 NLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKP 486
           NLY R+P+ NQ NA TAQGRTDPNQ+TGISIHNCTI  A + V   N T   YLGRPWK 
Sbjct: 431 NLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSS-NYTVKTYLGRPWKE 490

Query: 487 FSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNI 546
           +SRTV+MQSYI +++ PVGW EWNG   L TLYY E+ N G G++T+ RV W G+ ++N 
Sbjct: 491 YSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINS 550

Query: 547 TEAMNFTVYNFTMGDTWLPYTDIPFSGGLL 564
           T+A NFTV NF +GD W+  + +P+  GLL
Sbjct: 551 TDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of CmaCh04G014580 vs. TAIR10
Match: AT1G02810.1 (AT1G02810.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 464.9 bits (1195), Expect = 6.9e-131
Identity = 265/587 (45.14%), Postives = 361/587 (61.50%), Query Frame = 1

Query: 4   APFIFLFLLSLRLAG---ASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 63
           +P   L  LS  L     +S +   S+ +CK+T  PK C+S+      S  +   YG FS
Sbjct: 3   SPIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPH---SQGNVQQYGCFS 62

Query: 64  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEV-GALEDCRELSDSNVEFL-QSIDGVLEA 123
           +RK L Q+ K    +  Y+ R  ++ L++  V  AL+DCR L+   +++L  S + V + 
Sbjct: 63  IRKSLSQSRKFIRTVDRYIKR--NAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDT 122

Query: 124 AEGVDEELM-----ERIESILSAIVTNGQTCIDGLVESRS-----SLGNALSAPLLSAGE 183
           +     + +     + I+++LSA +TN QTC++GL  + S     ++   ++ PL++  +
Sbjct: 123 SAKTSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTK 182

Query: 184 LYSVSLGLVKNSLSRRW--KKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQST 243
           L  VSL L     ++ W  KK+ + G         ++   +P      G   +    + T
Sbjct: 183 LLGVSLAL----FTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPL-KMTEKT 242

Query: 244 KC---SGRKRMLTDLGTTG-------ILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQ 303
           K    S  +R L D  + G       +LI++ V VS  G  NFT+I  A++ APNN+   
Sbjct: 243 KAVYESLSRRKLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGS 302

Query: 304 DGYFVIYVRQGYYDEYVVVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYG 363
            G+F+IYV  G Y+EY+ + K K  +M+IGDGIN+TV+TGN +VVDGWTT+NS+TF V  
Sbjct: 303 AGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTA 362

Query: 364 DGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVY 423
             FVA+++TFRNTAG EKHQAVA+R+ AD S FY CSFE YQDTLY HSLRQFYRECDVY
Sbjct: 363 PNFVAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVY 422

Query: 424 GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWV 483
           GTVDFIFGNAA VFQ CNLY RKP+PNQ NA TAQGR+DPNQ+TG SI NCTI  A + V
Sbjct: 423 GTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV 482

Query: 484 MDLNETTTNYLGRPWKPFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPG 543
              N T   YLGRPWK +SRTVYMQSYI   + PVGW EWNG   L TLYY E+ N GPG
Sbjct: 483 SS-NYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPG 542

Query: 544 ANTSLRVDWDGHSLMNITEAMNFTVYNFTMGDTWLPYTDIPFSGGLL 564
           +NT+ RV W G+ ++N T+A NFTV    +   W+  T +P++ GL+
Sbjct: 543 SNTTNRVTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of CmaCh04G014580 vs. TAIR10
Match: AT2G47550.1 (AT2G47550.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 463.8 bits (1192), Expect = 1.5e-130
Identity = 252/558 (45.16%), Postives = 337/558 (60.39%), Query Frame = 1

Query: 12  LSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRKCLKQAIKM 71
           +S ++    ++  PS + C+    P  CRS+L +    P D Y YG+ S+R+ L +A + 
Sbjct: 22  ISAQIPAIGNATSPSNI-CRFAPDPSYCRSVLPN---QPGDIYSYGRLSLRRSLSRARRF 81

Query: 72  SSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELMERIE 131
            S+I   LDR G     +  VGALEDC+ L+   +++L S     ++ + +     E + 
Sbjct: 82  ISMIDAELDRKGKVAA-KSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDVH 141

Query: 132 SILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNSLSRRWKKRSQK 191
           + LSA +TN QTC++GL  + S   N LS  L +  +LY VSL L     S+ W  R Q+
Sbjct: 142 TFLSAAITNEQTCLEGLKSTASE--NGLSGDLFNDTKLYGVSLAL----FSKGWVPRRQR 201

Query: 192 G------DREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGTTGILI 251
                        F      + PL         M    ++   +  +R L       + +
Sbjct: 202 SRPIWQPQARFKKFFGFRNGKLPLK--------MTERARAVYNTVTRRKLLQSDADAVQV 261

Query: 252 NNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKTNIMLI 311
           ++ V V   G  NFT+I  AI+ APN +   +GYF+IYV  G Y+EYV VPK K  +M+I
Sbjct: 262 SDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMI 321

Query: 312 GDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSAD 371
           GDGIN+TVITGN +VVDGWTT+NS+TF + G  F+ +++T RNTAG  K QAVA+R+  D
Sbjct: 322 GDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGD 381

Query: 372 LSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQK 431
           LS FY CSFE YQDTLY HSLRQFYRECDVYGTVDFIFGNAA V Q CNLY R+P   Q 
Sbjct: 382 LSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQS 441

Query: 432 NAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYMQSYIG 491
           N  TAQGRTDPNQ+TG +IH CTI  A + +   N T   YLGRPWK +SRTV MQ+YI 
Sbjct: 442 NEVTAQGRTDPNQNTGTAIHGCTIRPADD-LATSNYTVKTYLGRPWKEYSRTVVMQTYID 501

Query: 492 DLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFTVYNFT 551
             + P GW  W+G   L TLYY E+ N GPG++T+ RV W G+ ++N T+A NFTV NF 
Sbjct: 502 GFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFL 559

Query: 552 MGDTWLPYTDIPFSGGLL 564
           +G+ W+  T +PF GGL+
Sbjct: 562 VGEGWIGQTGVPFVGGLI 559

BLAST of CmaCh04G014580 vs. NCBI nr
Match: gi|659091753|ref|XP_008446714.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Cucumis melo])

HSP 1 Score: 1002.3 bits (2590), Expect = 3.4e-289
Identity = 491/564 (87.06%), Postives = 525/564 (93.09%), Query Frame = 1

Query: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS PFIFL LLSL  AGAS SPP S  LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           V++C+KQAIKMS+VIG+YLDRG DSRLNR+E GAL DCRELSD NVEFL+SI+ VLEAAE
Sbjct: 61  VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 120

Query: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180
           GVDEEL+ER+ES LSAIVTNGQTC+DGLVESRSSLG ALSAPLLSAGELYSVSLGLV N+
Sbjct: 121 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 180

Query: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQ-STKCSGRKRMLTDLG 240
           +SRRWKKR +KG   GG  P   +SRE LNTLIKGLH M  CN  STKCSGRKR+LTDLG
Sbjct: 181 MSRRWKKRREKGGGNGG-VPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLG 240

Query: 241 TTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYK 300
           ++GILINNTVVVSSTGADNFTSIGDAI+FAPNNSMPQDGYFVIYVR+GYY+EYVVVPK+K
Sbjct: 241 SSGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFK 300

Query: 301 TNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVA 360
           TNIMLIGDGINRT+ITGNHNVVDGWTTYNSSTFTV GDGFVAID+TFRNTAG EKHQAVA
Sbjct: 301 TNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 VRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARK 420
           +RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECD+YGTVDFIFGNAAAVFQQCNLYARK
Sbjct: 361 LRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARK 420

Query: 421 PLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVY 480
           PLPNQKNAFTAQGRTDPNQ+TGISIHNCTI AAP+WVMD N TTTNYLGRPWK +SRTVY
Sbjct: 421 PLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTTTNYLGRPWKQYSRTVY 480

Query: 481 MQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNF 540
           MQSYIGDLISPVGWLEWNGTVGL+TLYYGE+ENYGPGANTSLRV+W G SLMN+T+AMNF
Sbjct: 481 MQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLMNLTQAMNF 540

Query: 541 TVYNFTMGDTWLPYTDIPFSGGLL 564
           TVYNFTMGDTWLPYTDIPFSGGLL
Sbjct: 541 TVYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of CmaCh04G014580 vs. NCBI nr
Match: gi|449435184|ref|XP_004135375.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Cucumis sativus])

HSP 1 Score: 999.6 bits (2583), Expect = 2.2e-288
Identity = 488/564 (86.52%), Postives = 526/564 (93.26%), Query Frame = 1

Query: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS PFIFL LLSL  AGAS SPPPS  LCKSTLYPKLCRSILS+IRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPPSTALCKSTLYPKLCRSILSTIRFSPSDPYGYGKFS 60

Query: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           V++C+KQA KMS+VIG+YL+RG DSRLNR E GAL DCR+LSD NVEFL+SI+ VLEAAE
Sbjct: 61  VKQCIKQATKMSTVIGDYLNRGRDSRLNRPEAGALSDCRDLSDLNVEFLRSIERVLEAAE 120

Query: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180
           GVDEEL+ER+ESILSAIVTNGQTCIDGLVESRSSLGNALS PLLSAGELYSVSLGLV N+
Sbjct: 121 GVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSGPLLSAGELYSVSLGLVSNA 180

Query: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCN-QSTKCSGRKRMLTDLG 240
           +SRRWKKR +KG   GG  P   +SREPL+TLIKGLH M  CN QSTKC GR+R+LTDLG
Sbjct: 181 MSRRWKKRREKGGGNGG-VPGGGRSREPLDTLIKGLHKMEPCNNQSTKCLGRQRLLTDLG 240

Query: 241 TTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYK 300
           +TGILINNTVVVSSTGADNFTSIGDAI+FAPNNSMPQDGYFVIYV++GYY+EYVVVPK+K
Sbjct: 241 STGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVKEGYYEEYVVVPKFK 300

Query: 301 TNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVA 360
           TNIMLIGDGINRT+ITGNHNVVDGWTTYNSSTFTV GDGFVAID+TFRNTAG EKHQAVA
Sbjct: 301 TNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 VRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARK 420
           +RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECD+YGTVDFIFGNAAAVFQQCNLYARK
Sbjct: 361 LRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARK 420

Query: 421 PLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVY 480
           PLPNQKNAFTAQGRTDPNQ+TGISIHNCTI AAP+WVMD N TTTNYLGRPWK +SRTVY
Sbjct: 421 PLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTTTNYLGRPWKQYSRTVY 480

Query: 481 MQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNF 540
           MQSYIGDLISPVGWLEWNGTVGL+TLYYGE+ENYGPGANTSLRV+W G SL+N+T+AMNF
Sbjct: 481 MQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLLNVTQAMNF 540

Query: 541 TVYNFTMGDTWLPYTDIPFSGGLL 564
           TVYNFTMGDTWLPYTDIPFSGGLL
Sbjct: 541 TVYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of CmaCh04G014580 vs. NCBI nr
Match: gi|255549442|ref|XP_002515774.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Ricinus communis])

HSP 1 Score: 769.6 bits (1986), Expect = 3.7e-219
Identity = 391/572 (68.36%), Postives = 461/572 (80.59%), Query Frame = 1

Query: 2   NSAPFIFLFLLSLRLAGASDSPPPS-----AVLCKSTLYPKLCRSILSSIRFSPSDPYGY 61
           N + F  L  LS  L  +S SPP S     ++ CKS+LYPKLCRSILS+ R SPSD Y Y
Sbjct: 3   NLSLFFALLFLSSALLISSQSPPQSQSQSPSLACKSSLYPKLCRSILSTYRSSPSDLYDY 62

Query: 62  GKFSVRKCLKQAIKMSSVIGEYLDRGGD-SRLNRQEVGALEDCRELSDSNVEFLQSIDGV 121
            KFSV++C+KQA ++S  I  YL      S++N +E+GALEDC EL+  NV++L +I   
Sbjct: 63  SKFSVKQCIKQANRLSKAINYYLTHDKHRSKINSKEIGALEDCHELTQLNVDYLGTISSE 122

Query: 122 LEAAEGVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLG 181
           L++AE +++EL+ER+ S+LS IVTN QTC DGLVES+SS+   L APL +   LYSVSLG
Sbjct: 123 LKSAESMNDELVERVTSLLSGIVTNQQTCYDGLVESKSSIVAVLQAPLTNVTRLYSVSLG 182

Query: 182 LVKNSLSRRWKK--RSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQS--TKCSGR 241
           LV ++L R  KK  R++KG   G       + REPLNTLIK L    SC+ S  ++C   
Sbjct: 183 LVTHALDRNLKKNKRNKKGSH-GKGILTKNRIREPLNTLIKALRK-SSCHTSGGSRC--- 242

Query: 242 KRMLTDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDE 301
           +R L D+   GILIN+TV+VS  G DNFTSIGDAI+ APNNS P+DGYFVIY R+GYY+E
Sbjct: 243 RRNLADMEEDGILINDTVIVSPYGTDNFTSIGDAIAIAPNNSKPEDGYFVIYAREGYYEE 302

Query: 302 YVVVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAG 361
           YV+VPKYK NI+LIGDGINRTVITGNH+VVDGWTT+NSST  V G+ FVA+D+TFRNTAG
Sbjct: 303 YVIVPKYKKNILLIGDGINRTVITGNHSVVDGWTTFNSSTVAVSGERFVAVDVTFRNTAG 362

Query: 362 AEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQ 421
            +KHQAVA+RN+ADLSTFYRCSFEGYQDTLYVHSLRQFYRECD+YGTVDFIFGN+AAVFQ
Sbjct: 363 PQKHQAVALRNNADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNSAAVFQ 422

Query: 422 QCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPW 481
            CNLYARKPLPNQKNAFTAQGRTDPNQ+TGISIHNCTI AAP+  MDLN +T N+LGRPW
Sbjct: 423 SCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIEAAPDLAMDLN-STLNFLGRPW 482

Query: 482 KPFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLM 541
           K +SRTV+MQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTS+RV W G++LM
Sbjct: 483 KQYSRTVFMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSMRVQWPGYNLM 542

Query: 542 NITEAMNFTVYNFTMGDTWLPYTDIPFSGGLL 564
           N+++A NFTVYNFTMGDTWLP TDIPFSGGLL
Sbjct: 543 NVSQAANFTVYNFTMGDTWLPETDIPFSGGLL 568

BLAST of CmaCh04G014580 vs. NCBI nr
Match: gi|802667492|ref|XP_012081346.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Jatropha curcas])

HSP 1 Score: 749.6 bits (1934), Expect = 4.0e-213
Identity = 378/569 (66.43%), Postives = 449/569 (78.91%), Query Frame = 1

Query: 1   MNSAPFIFLFL-LSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKF 60
           ++S+ FIF  L LS  L  +SD     ++ CKST YPKLCRSILS+ RFS SDPY YGKF
Sbjct: 4   LSSSFFIFTLLSLSSFLLVSSDQSQSPSIACKSTPYPKLCRSILSTFRFSSSDPYDYGKF 63

Query: 61  SVRKCLKQAIKMSSVIGEYLDRGG-DSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEA 120
           SV++CLKQ  ++S  I  YL      S+L+R+E+GAL DC EL+  +V++L++I   L+A
Sbjct: 64  SVKQCLKQTHRLSKAINYYLTHDKYRSKLSREEIGALTDCHELTQLSVDYLETISTELQA 123

Query: 121 AEGVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVK 180
           A  +  EL+ER+ S+LS +VTN  TC DGLV+S+SS+   L  P+ +  +LYSVSLGLV 
Sbjct: 124 AASLSGELVERVTSLLSGVVTNQDTCFDGLVDSKSSIAGGLYNPIANVSDLYSVSLGLVT 183

Query: 181 NSLSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLH-SMGSCNQSTKCSGRK---RM 240
           ++L R  KK   K  +   +  +    REPL++LIK LH +  SC++S     RK   R 
Sbjct: 184 HALDRNLKK--NKRSKNQRSVFSKKGVREPLDSLIKALHKTKSSCHKSGGSDCRKKVGRN 243

Query: 241 LTDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVV 300
           L D  + GILIN+TV+VS  G  NFTSIGDAI+ APNNS  +DGYFVIY R+GYY+EYVV
Sbjct: 244 LADTESGGILINDTVIVSPYGDYNFTSIGDAIAIAPNNSKQEDGYFVIYAREGYYEEYVV 303

Query: 301 VPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEK 360
           VPKYK NI+LIGDGINRTVITGNH+VVDGWTTYNSSTF V G+ F+A+D+TFRNTAG  K
Sbjct: 304 VPKYKKNILLIGDGINRTVITGNHSVVDGWTTYNSSTFAVSGERFIAVDITFRNTAGPAK 363

Query: 361 HQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCN 420
           HQAVAVRN+ADLSTFYRCSFEGYQDTLYVHSLRQFYRECD+YGTVDFIFGNAAAVFQ CN
Sbjct: 364 HQAVAVRNNADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQSCN 423

Query: 421 LYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPF 480
           LY RKPLPNQKNA TAQGRTDPNQ+TGISIHNCTI AAP+  M++N +T NYLGRPWK +
Sbjct: 424 LYTRKPLPNQKNAITAQGRTDPNQNTGISIHNCTIEAAPDLAMEVNSSTLNYLGRPWKEY 483

Query: 481 SRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNIT 540
           SRTVYM+SYIGDLI   GWL WNGTVGLDTLYYGEF+NYGPGANTSLRV W G+S+MN++
Sbjct: 484 SRTVYMESYIGDLIDKNGWLAWNGTVGLDTLYYGEFDNYGPGANTSLRVQWGGYSIMNVS 543

Query: 541 EAMNFTVYNFTMGDTWLPYTDIPFSGGLL 564
           EA+NFTVYNFTMGDTWLP TDIPF GGLL
Sbjct: 544 EAVNFTVYNFTMGDTWLPNTDIPFYGGLL 570

BLAST of CmaCh04G014580 vs. NCBI nr
Match: gi|743906137|ref|XP_011046483.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Populus euphratica])

HSP 1 Score: 746.9 bits (1927), Expect = 2.6e-212
Identity = 372/561 (66.31%), Postives = 445/561 (79.32%), Query Frame = 1

Query: 6   FIFLFLLSLRLAGASDSPPPSAV--LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRK 65
           F FL +LS  L  +   PPP +    CKSTLYPKLCRSIL++   S S+PY Y KFSV++
Sbjct: 11  FAFLSILSPSLISSQSPPPPYSPSNACKSTLYPKLCRSILTTFPSSSSNPYEYSKFSVKQ 70

Query: 66  CLKQAIKMSSVIGEYLDRGGD-SRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGV 125
           CLKQA ++S VI  +L      S++ R+E GAL+DC EL + NV+F  +I   L AA+ +
Sbjct: 71  CLKQAKRLSKVIDYHLTHKKQLSKMTREEFGALQDCHELMELNVDFFVTISNELVAAKSM 130

Query: 126 DEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNSLS 185
            + L+ER+ S+LS +VTN QTC DGLV+S+SS+  ALSAPL +  +LYSVSL LV +SL 
Sbjct: 131 SDVLVERVTSLLSGVVTNQQTCYDGLVQSKSSIAGALSAPLSNVTQLYSVSLALVAHSLE 190

Query: 186 RRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTD-LGTT 245
           +  KK  ++     G        REPL TL+K L    SC++S  C   +R+L+D  G  
Sbjct: 191 KNLKKNKRRKGSPQGTLTRGI--REPLETLVKALRKTSSCHESRNCHRGERILSDDAGGD 250

Query: 246 GILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKTN 305
           GIL+++TV+VS  G DNFT+IGDAI+FAPNNS P+DGYFVI+VR+G Y+EYVVVPK K N
Sbjct: 251 GILVHDTVIVSPYGTDNFTTIGDAIAFAPNNSKPEDGYFVIFVREGTYEEYVVVPKNKKN 310

Query: 306 IMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVR 365
           I+LIG+GINRTVITGNH+V+DGWTT+NSSTF V G+ FV +D+TFRNTAG EKHQAVA+R
Sbjct: 311 IVLIGEGINRTVITGNHSVIDGWTTFNSSTFAVSGERFVGVDITFRNTAGPEKHQAVALR 370

Query: 366 NSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPL 425
           N+ADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQ CNLYARKP+
Sbjct: 371 NNADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQNCNLYARKPM 430

Query: 426 PNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYMQ 485
            NQKNAFTAQGRTDPNQ+TGISIHNCTI AAP+  MD N +T N+LGRPWK +SRTV MQ
Sbjct: 431 LNQKNAFTAQGRTDPNQNTGISIHNCTIEAAPDLAMDRN-STLNFLGRPWKEYSRTVIMQ 490

Query: 486 SYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFTV 545
           SYIG+LI PVGWLEWNGTVGLDT+YYGEF+NYGPGANTS RV W G +LMN T+A+NFTV
Sbjct: 491 SYIGELIQPVGWLEWNGTVGLDTIYYGEFQNYGPGANTSRRVQWPGFNLMNTTQAVNFTV 550

Query: 546 YNFTMGDTWLPYTDIPFSGGL 563
           YNFTMGDTWLPYTD+PFSGGL
Sbjct: 551 YNFTMGDTWLPYTDVPFSGGL 568

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME25_ARATH1.1e-19159.82Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana GN=P... [more]
PME47_ARATH2.8e-19060.29Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana GN=P... [more]
PME41_ARATH7.0e-14147.89Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana GN=P... [more]
PME7_ARATH1.2e-12945.14Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana GN=PM... [more]
PME20_ARATH2.7e-12945.16Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana GN=P... [more]
Match NameE-valueIdentityDescription
A0A0A0KUN8_CUCSA1.5e-28886.52Pectinesterase OS=Cucumis sativus GN=Csa_5G612810 PE=4 SV=1[more]
B9RPV5_RICCO2.6e-21968.36Pectinesterase OS=Ricinus communis GN=RCOM_1541960 PE=4 SV=1[more]
A0A067K1K9_JATCU2.8e-21366.43Pectinesterase OS=Jatropha curcas GN=JCGZ_18594 PE=4 SV=1[more]
B9HVF5_POPTR1.9e-20965.43Pectinesterase OS=Populus trichocarpa GN=POPTR_0010s25400g PE=4 SV=2[more]
D7TF25_VITVI4.2e-20965.43Pectinesterase OS=Vitis vinifera GN=VIT_13s0047g00230 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G10720.26.4e-19359.82 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G04970.11.6e-19160.29 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G02330.13.9e-14247.89 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT1G02810.16.9e-13145.14 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT2G47550.11.5e-13045.16 Plant invertase/pectin methylesterase inhibitor superfamily[more]
Match NameE-valueIdentityDescription
gi|659091753|ref|XP_008446714.1|3.4e-28987.06PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Cucumis melo][more]
gi|449435184|ref|XP_004135375.1|2.2e-28886.52PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Cucumis sativus][more]
gi|255549442|ref|XP_002515774.1|3.7e-21968.36PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Ricinus communis... [more]
gi|802667492|ref|XP_012081346.1|4.0e-21366.43PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Jatropha curcas][more]
gi|743906137|ref|XP_011046483.1|2.6e-21266.31PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Populus euphrati... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000070Pectinesterase_cat
IPR006501Pectinesterase_inhib_dom
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
GO:0004857enzyme inhibitor activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005618 cell wall
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G014580.1CmaCh04G014580.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 249..548
score: 1.3E
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 29..179
score: 3.8
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 29..174
score: 1.8
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 20..176
score: 1.1
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 29..179
score: 2.35
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 244..561
score: 5.93E
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 246..558
score: 2.4E
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 230..563
score: 0.0coord: 1..214
score:
NoneNo IPR availablePANTHERPTHR31707:SF21PECTINESTERASE/PECTINESTERASE INHIBITOR 25-RELATEDcoord: 230..563
score: 0.0coord: 1..214
score: