ClCG05G022100 (gene) Watermelon (Charleston Gray)

NameClCG05G022100
Typegene
OrganismCitrullus lanatus (Watermelon (Charleston Gray))
DescriptionPectinesterase
LocationCG_Chr05 : 34085854 .. 34092517 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACTCAGTTCCATTCATTTGCCTTCTCCTTCTCTCTCTCCTTCTCGCCGGAGCTTCCGATTCTTCTCCGCCGTCTACCGCCCTCTGTAAATCCACTCTCTACCCTAAGCTCTGCCGCTCCATTCTCTCATCCATCCGGTTCTCCCCCTCCGATCCATACGGCTACGGGAAATTCTCCGTCAAGAAATGTCTCAAACAAGCGATCAAAATGTCGAGCGTAATCAGCGACTATCTCGACCGAGGACGCGATTCGAGGCTGAATCAGCGGGAGGCCGGCGCTCTGAATGACTGCAGAGAACTTTCCGATTTGAATGTGGAGTTTCTTCGGTCGATCCAGGGCGTATTGGAGGCGGCGGAGGGCGTCGATGAGGAACTGGTGGAGCGCGTTGAGAGTATTCTGAGCGCGATCGTGACGAACGGCCAGACTTGTATCGATGGATTGGTGGAATCGAGGAGTAGTTTGGGGAATGCGCTATCGGCGCCGCTGGCGAGCGCCGGAGAGCTGTACAGTGTGTCATTGGGATTGGTTTCGAACTCGCTGTCGCGACGGTGGAAGAAGCGGCGGCAGAAGGGAAATGGAGATGGAGGGAATTTTCCGGCGGGTGCGCGGAGCAGAGAGCCATTGAACACGCTGATCAAGGTACGATTGAATATGGGTGTTGTTGCTGTTTGGTTTCTGTTTGTCTGTTCGTTCGGTTACACGAACCATTGCATTTGTTTTATTCAATGCATAAAATAATTGGGGAAAAGAAAGTTTTTGTTTGGACGTAGCCAATAGCCATTAATTTAGATTTTTTTTTTCTTTAAATTACATATTAGTTTTTTAATTTTTAAATTCGTGTCATTTTTAATATTACAACTTTTAGTTGTAATTATATCATCAAATTTTTAATAATAAAAATTGAGACCTCAAACTAATATAAGTATTAAAATTGAACTTTTAAACTTACATAATTTTAGAAATTGTATAAGTTCAAGATTTCAATTTTTATTATTTGAGGATCCACTTTCAACAACTATTGTAAGTTTGGGATACAATTTTAACCTTTGTATAAGTCTAAGGTTTCAATTTTTACAATTAAGTAGTTCAAACTACTATAATATTTCAAGAATGCTTTTTATAATTTGTCTTATTTTTAAAATTAGCAGATTTACCATATGCAAAATTAAAAGTTTAGATTTTGTTAAACATAAAATTTTAAATTAAATTAAAAAAAAATTTGAATTTGCGCTTTATTCACATAATTTGAAGGAACTTAGGTATGTACTTCTGAAAATTCACAGTTAAATAGGCACAAATTTCAATTTACGTGGACTAAACTGTAATTTCACTTTTTTTGTTTTAATAAAAGAACAACAAAGCATAATATTTAATTTAAACTTTTTGGAAAATTTAAAATGTTGGGGATTCCACCCTTACTTTTGTAATTGTTAAGTTAAAAACATTATTATCTTAAAATTAAAAACTCAACCATAAAACTTCATATCTAATAAATTCTTACAATTTCAATGTGGCATTTAGCATGAAGTTCTTGAACATTCAATATTATATTTAATAAGACTTTAAATGTTAAAATATATAATAAGTTTGAAACTTTTAATTTTGTATTTAGTATCATAGTAGATTAATTTCGTAAAACAAATTTTAGAATTTAAATACTTAATAAATACAAAATTACAAGTTGAGAAGTATATTATACACTTTTAAGATTAATTTACTTATTAAACACAATAATAAAAATGTATGGACCTATTAAATATTTTTTATATAAAAATTTGATTTATTAGACATCTAAAATTTATGGATTGAACTTTCAATTTACCTAAATTTATTAGCTCAACCAAATTCCATTTTACACTATTACAATTTTTTTTTTAATTCAACAATTGCCAGGCGGGGAGTAAACCTATCCAGTATCAACTATTAAAAGTAATTAATGACAACAGGGTTGCATTAAAACTTTATTTTCTCTTTCAAAAAAAAACTTTATTTTCTTAATACAAAGTACATAGTTTCATTCAAATTATTTTATTTTATTCATTTCTTAAAATAAAAAATAAAAAATAAAAAAAAGCCAAAATATCAAATCAGACGTCAGGATAAATGAACGTCTGACTTTTGAGACAAAGTATTGGCAGAAGACAGAAATAATATGTTTTTTTTAAATTTATTTTTTTAAAAAAATTATTTTAAATGACAAAAATATTGAAAAAAATTATCAATAATAAATTGAGATAAATTAATATCAGTATCTATCATGACCTAGATCAGCACACATAACAGTAGTAGTGTAGATAATAAAATTTTGTTATATTTATAAATTTCCTATATTTGAAAACAACTTATTTTAAAGATAATTTAATATTTGAATTTTTATCCAATAGTGTAGATGATTAAAAATAAATATTTTTAATGGTTATATTTTATGATAAAATGACAAATTAGACATCAGGATTGAGGAACTCCTCAAAAGGGCATGAGGGGATCAAAGAAGCTATATGATCAATCAAACGCGAAATTAAAATAAATCACAAAAATGTTTTTTTTAGAGAAATAAATCACAAAAATATTTTTTTAGTGAAACATAAATTCTTTTATTATGATGCATTCTTTTAGTATTTAAGTATTTAAATTAATTTTACTTTGTTGGTAAAAAAATTTCTCCAACCAAGAATCAGCATGGTACCATTTTGTTTCTATTATTAATTCTTTATTAAAGTGAAAGACAAGCTTGTTTATCAATATTGGATCCCCTTTACAAAATATGTTATTGTCGAAATTTAAAAAAAAAAAAAATACAACTATTGTAAATGATCTTTAAAATATATTAGAAAAAAATTTAAATAATATTTTTAATCTCTAGTGACAATTCAATTTGTAATAATTTAGTTTTTATCATAAAAAACTATTATTAAGAATTAATAGATATTTCTTATACATATAAATTGATAAACTAACTAAGAATCAAATATATTTATAAATTATAAAAACTAAATAGTTATAAATCAAAATGTATAGATACTAAATTGTTGCTTCTCTTAATACTAGAACACGAAAAGTGTTTATAAAAATCATACTTTATCAAACTAAAATTCAAATGGTTTCTAATTTGAGACCTTAAATAAAAAAAACAACCAAATAAATGAAAACTCGGGCTATCTTGTGACTTTTTTTTTTTTTTTTTTAATTAGATTCTATCCTGCTTTTACAAAACCGTATATTTATCTCTTTAAATAATTTTAATTAACTTGTGAAATTGGGAGAAGAAATGTTAGATAATTGCAAATATTAGTGGAATATAATATAAAAATTATGAACAATTTTAAGTTAATCAGCATTTAATCAATTGTTGATGTCTGTTAATTGGAATATTAGGACAAATAAAGAGGGTAAAGTTCCTGTAGACTGTGGAGGCATCTGCAACCCTATCCTTAATACTACAAAATCCCAATTTAAAAAGATAATATTTATTTGATTAAAATAATAATAATAATGAATTTTTGTTTATTAAATTGTGTTAAATATGTTGATAAGATTGTTGGTATCACGAGAGAGATTTTAGTGGGGACGGCAGAGTAGGATTCCCATAGAGAGAATTAATCAAATGCATGTTACAAATTGAGATGTGGATTCTCTTCACAATTTTAAATTAGCTTATTCTAATTACTTTATTCCTATATCATTTGCCTCTTAATATGTTTATTTATATATATCTTTTGAGTTCAACCATGTAGAATAGGAATTTCAACTCGTAAGCTTTTTGGTCGACGTTATTAATATCTTAACCTGTTGACCGTTGATTTTTTTAAATTTAATTTTTATTTTATTTTAAATGAAAGGAATCCGTTGAACTATATTATATTGACGGTTATTTACTTTATTGTTTCATCAAATATTATTCTTGGACAAACTGAGTTACTGAGCCAATAAGGTTTTATATATATATATATATATATATAAATTAAAGGGATATTTTAAACCTATTTGACAACTATTTGTATCTCATTTGGTAAGCATTTTGATTTTAACTTATTTTAATATAATTAAAAGGTTTATAAACATTACTTTTACCTTTTGTCATCCGTTTTTACAAATATTTTGAAAACCTAAAAAAAAATATTTTTAAAATTTATCTAAGAATTCAAGTGTTTATTCAATAAATGTGAAAACTATACTACAAAAATTGAGAAAAAACAAAGTATTGATTTTTGGTTTTTGGTTTTTGGAAATTAGGCTTATAAATACTTTCAACCTATAAGTTTATTTGTTCTTTTGATAACATGTAGGTAGAAGCAATCGAATCTCTGACTTCACGGTTGATAATATAAATACTATGGTAGAGCTAGGTTTATTTTGGCCTATAAATTTATTTGTCTTGTCATTTACTTAGACTAAAAGTTTAAAGTTTTCTCTAAGAAAAGATGAGAACTATTATAATGCGATGATGAACTTAGTTGCTGATTTTAGAGGTGAATTCAATTATTTTAAGGAACAATTAACTTCAAGGTTTAAGTTGAATCAGGGACTACATAAAATGGGATCATGCAACAACCAATCCACAAAGTGCTCAGGAAGGAAGCGTTTGTTAACTGATTTAGGCAGCACAGGAATTTTGATCAACAACACTGTGGTTGTTAGCTCAACTGGTGCTGACAACTTCACATCAATTGGAGATGCAATTGCATTTGCTCCCAACAACTCAATGCCTCAAGATGGCTACTTTGTTATCTATGTTAGAGAAGGATACTATGAAGAATACGTAGTCATCCCCAAATTCAAAACCAATGTAATGCTCATCGGAGACGGCATTAATCGAACCATCATCACCGGGAACCATAGCGTCGTCGATGGTTGGACGACTTATAACTCTTCAACCTTCAGTAAGCATATCACAAATTCATTACTCGTCTAATTTTATCTTCACTCCCTCCCCCTTGATGAACGTACAAATCACATGATCACAATTCTTAAAAAGTTAAATAACAAAAGGTATTATAAACTTGGTTCTTTGTTCTTTCAAAAGTTGTGATATTACTCTTGAAGTAAAATCTATTAGAATTTTGGATGAAAATTTAGAACTGAAACGATATGCAGTTGACCTGAAACGGTATGACGGTGGCGATTTTCGTTCTAGTTGCTCACTGCGACACGACACCGTTTGAAGTCCTAGTTTTCATTCAACATTCAAATAGACCTTTACACTAGTGATATTTTTTAAATATTTATGAAACATCAATGGGTAATATTACAACTTTTGAACAAATATGAGTATTTTTTAAACAAATGGAAAGAGTTCAGAATTGGTAGTTTGAATGTTTTGGACATATTTTGCCTAATCATTTTATCACAATTCTTGAAATTGTTAAATTACAAAATGTATTCATTAACTTTCTTCTTTGTTCTTCGAAGAGTTGAAATACTGAAGTAGAAATCTATTAGAGTTTTGGATAAAAATTGAGACTTGAAAGCAATAATTTGTATTAGAAAACAATATGCTAATGGCCTAACATGATATAACAGTCTCAATTTTCGTTCGAAGTCACTCCCACGCCCTCCCAATTTCTAACATTCTAAGGAATTTCTACTTCAAAATAGTATTGAAATGCTTCTAGACAAAGTTGAAGGGTATATATCTTTTTATAATACACCTTTGATAAAAAGAGTTTGGAATGGGTAGTTTGAATGTTTTGAACATATATTTTGCAGCTGTTTGTGGGGATGGATTTGTTGCAATAGACATGACATTCAGGAACACAGCAGGGCCAGAGAAGCACCAAGCTGTAGCAGTAAGAAACAGTGCAGATCTCTCAACATTCTACCGCTGTAGTTTTGAAGGATATCAAGACACTCTTTATGTTCATTCCCTAAGACAATTCTATAGAGAATGTGACATTTATGGCACTGTAGATTTCATTTTTGGCAATGCAGCTGCAGTTTTCCAGCAATGCAATCTCTATGCCAGAAAGCCACTCCCCAACCAAAAAAATGCCTTCACCGCGCAGGGCCGAACCGATCCGAACCAAAACACAGGCATCTCGATCCACAACTGCAGCATCAAGGCTGCACCCGACTGGGCGATGGATTGGAATGCGACGACGATGACGACGAACTACCTCGGGCGACCGTGGAAACAGTACTCAAGGACAGTGTATATGCAGTCTTATATTGGGAATTTGATAAGCCCAGTGGGATGGTTGGAATGGAATGGAACAGTAGGATTAGAGACATTGTATTATGGGGAGTTTGAGAATTATGGACCAGGAGCTAACACAAGCTTGAGGGTGAATTGGCCTGGATATAGCTTGATGAATCTTACACAAGCTATGAATTTTACTGTCTATAATTTCACTATGGGAGATACTTGGCTGCCTTATACTGATATACCTTTCTCTGGAGGTTTGTTAATTATGTGATTTTTCCTCTTAAATTCATCTTTCACTTTCAAGCTTTTCATCTTTTATTCAGAGGGAAATGGAGAGGGATTTTAGACCTGGGGGGCTTCAGTGTTGAGTTGCTATGGCTTTGGATATATACTTTGTATAAATCATTGGTGTTTGCAACTCTTAGAGAGAGCTCTAATTATTTTGAAAAGAGAGAGTATTTGGTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTAGTTTTTGAAATTTATGTTTAA

mRNA sequence

ATGAACTCAGTTCCATTCATTTGCCTTCTCCTTCTCTCTCTCCTTCTCGCCGGAGCTTCCGATTCTTCTCCGCCGTCTACCGCCCTCTGTAAATCCACTCTCTACCCTAAGCTCTGCCGCTCCATTCTCTCATCCATCCGGTTCTCCCCCTCCGATCCATACGGCTACGGGAAATTCTCCGTCAAGAAATGTCTCAAACAAGCGATCAAAATGTCGAGCGTAATCAGCGACTATCTCGACCGAGGACGCGATTCGAGGCTGAATCAGCGGGAGGCCGGCGCTCTGAATGACTGCAGAGAACTTTCCGATTTGAATGTGGAGTTTCTTCGGTCGATCCAGGGCGTATTGGAGGCGGCGGAGGGCGTCGATGAGGAACTGGTGGAGCGCGTTGAGAGTATTCTGAGCGCGATCGTGACGAACGGCCAGACTTGTATCGATGGATTGGTGGAATCGAGGAGTAGTTTGGGGAATGCGCTATCGGCGCCGCTGGCGAGCGCCGGAGAGCTGTACAGTGTGTCATTGGGATTGGTTTCGAACTCGCTGTCGCGACGGTGGAAGAAGCGGCGGCAGAAGGGAAATGGAGATGGAGGGAATTTTCCGGCGGGTGCGCGGAGCAGAGAGCCATTGAACACGCTGATCAAGGGACTACATAAAATGGGATCATGCAACAACCAATCCACAAAGTGCTCAGGAAGGAAGCGTTTGTTAACTGATTTAGGCAGCACAGGAATTTTGATCAACAACACTGTGGTTGTTAGCTCAACTGGTGCTGACAACTTCACATCAATTGGAGATGCAATTGCATTTGCTCCCAACAACTCAATGCCTCAAGATGGCTACTTTGTTATCTATGTTAGAGAAGGATACTATGAAGAATACGTAGTCATCCCCAAATTCAAAACCAATGTAATGCTCATCGGAGACGGCATTAATCGAACCATCATCACCGGGAACCATAGCGTCGTCGATGGTTGGACGACTTATAACTCTTCAACCTTCACTGTTTGTGGGGATGGATTTGTTGCAATAGACATGACATTCAGGAACACAGCAGGGCCAGAGAAGCACCAAGCTGGCCGAACCGATCCGAACCAAAACACAGGCATCTCGATCCACAACTGCAGCATCAAGGCTGCACCCGACTGGGCGATGGATTGGAATGCGACGACGATGACGACGAACTACCTCGGGCGACCGTGGAAACAGTACTCAAGGACAGTGTATATGCAGTCTTATATTGGGAATTTGATAAGCCCAGTGGGATGGTTGGAATGGAATGGAACAGTAGGATTAGAGACATTGTATTATGGGGAGTTTGAGAATTATGGACCAGGAGCTAACACAAGCTTGAGGGTGAATTGGCCTGGATATAGCTTGATGAATCTTACACAAGCTATGAATTTTACTGTCTATAATTTCACTATGGGAGATACTTGGCTGCCTTATACTGATATACCTTTCTCTGGAGTTTTTGAAATTTATGTTTAA

Coding sequence (CDS)

ATGAACTCAGTTCCATTCATTTGCCTTCTCCTTCTCTCTCTCCTTCTCGCCGGAGCTTCCGATTCTTCTCCGCCGTCTACCGCCCTCTGTAAATCCACTCTCTACCCTAAGCTCTGCCGCTCCATTCTCTCATCCATCCGGTTCTCCCCCTCCGATCCATACGGCTACGGGAAATTCTCCGTCAAGAAATGTCTCAAACAAGCGATCAAAATGTCGAGCGTAATCAGCGACTATCTCGACCGAGGACGCGATTCGAGGCTGAATCAGCGGGAGGCCGGCGCTCTGAATGACTGCAGAGAACTTTCCGATTTGAATGTGGAGTTTCTTCGGTCGATCCAGGGCGTATTGGAGGCGGCGGAGGGCGTCGATGAGGAACTGGTGGAGCGCGTTGAGAGTATTCTGAGCGCGATCGTGACGAACGGCCAGACTTGTATCGATGGATTGGTGGAATCGAGGAGTAGTTTGGGGAATGCGCTATCGGCGCCGCTGGCGAGCGCCGGAGAGCTGTACAGTGTGTCATTGGGATTGGTTTCGAACTCGCTGTCGCGACGGTGGAAGAAGCGGCGGCAGAAGGGAAATGGAGATGGAGGGAATTTTCCGGCGGGTGCGCGGAGCAGAGAGCCATTGAACACGCTGATCAAGGGACTACATAAAATGGGATCATGCAACAACCAATCCACAAAGTGCTCAGGAAGGAAGCGTTTGTTAACTGATTTAGGCAGCACAGGAATTTTGATCAACAACACTGTGGTTGTTAGCTCAACTGGTGCTGACAACTTCACATCAATTGGAGATGCAATTGCATTTGCTCCCAACAACTCAATGCCTCAAGATGGCTACTTTGTTATCTATGTTAGAGAAGGATACTATGAAGAATACGTAGTCATCCCCAAATTCAAAACCAATGTAATGCTCATCGGAGACGGCATTAATCGAACCATCATCACCGGGAACCATAGCGTCGTCGATGGTTGGACGACTTATAACTCTTCAACCTTCACTGTTTGTGGGGATGGATTTGTTGCAATAGACATGACATTCAGGAACACAGCAGGGCCAGAGAAGCACCAAGCTGGCCGAACCGATCCGAACCAAAACACAGGCATCTCGATCCACAACTGCAGCATCAAGGCTGCACCCGACTGGGCGATGGATTGGAATGCGACGACGATGACGACGAACTACCTCGGGCGACCGTGGAAACAGTACTCAAGGACAGTGTATATGCAGTCTTATATTGGGAATTTGATAAGCCCAGTGGGATGGTTGGAATGGAATGGAACAGTAGGATTAGAGACATTGTATTATGGGGAGTTTGAGAATTATGGACCAGGAGCTAACACAAGCTTGAGGGTGAATTGGCCTGGATATAGCTTGATGAATCTTACACAAGCTATGAATTTTACTGTCTATAATTTCACTATGGGAGATACTTGGCTGCCTTATACTGATATACCTTTCTCTGGAGTTTTTGAAATTTATGTTTAA

Protein sequence

MNSVPFICLLLLSLLLAGASDSSPPSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKKCLKQAIKMSSVISDYLDRGRDSRLNQREAGALNDCRELSDLNVEFLRSIQGVLEAAEGVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLASAGELYSVSLGLVSNSLSRRWKKRRQKGNGDGGNFPAGARSREPLNTLIKGLHKMGSCNNQSTKCSGRKRLLTDLGSTGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPKFKTNVMLIGDGINRTIITGNHSVVDGWTTYNSSTFTVCGDGFVAIDMTFRNTAGPEKHQAGRTDPNQNTGISIHNCSIKAAPDWAMDWNATTMTTNYLGRPWKQYSRTVYMQSYIGNLISPVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGVFEIYV
BLAST of ClCG05G022100 vs. Swiss-Prot
Match: PME47_ARATH (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana GN=PME47 PE=2 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 9.2e-129
Identity = 267/547 (48.81%), Postives = 332/547 (60.69%), Query Frame = 1

Query: 24  PPSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKKCLKQAIKMSSVISDYLDR-- 83
           PP+ A CKST YPKLCR+ILS+++ SPSDPY YGKF++K+CLKQA ++S VI+ +  R  
Sbjct: 83  PPNIA-CKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQRVE 142

Query: 84  GRDSRLNQREAGALNDCRELSDLNVEFLRSIQGVLEAAEGVDEELVERVESILSAIVTNG 143
                    E  A+ DC EL++L+VE+L ++   L+AAE +   LV+RV S+L  +VTN 
Sbjct: 143 ADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAALVDRVTSLLGGVVTNQ 202

Query: 144 QTCIDGLVESRSSLGNALSAPLASAGELYSVSLGLVSNSLSRRWKKRR-QKGN-GDGGNF 203
           QTC+DGLV+++S    A+  PL +   LYSVSLGLVS++L+R  K+ +  KG    GGN 
Sbjct: 203 QTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSKGKIFGGGNK 262

Query: 204 PAGARSREPLNTLIKGLHKMGSCNNQSTKCSGRKRLLTDLGSTG---ILINNTVVVSSTG 263
           P     REPL TLIK L K  +C+ +   C    R L +LG T    IL+   V V    
Sbjct: 263 PV----REPLETLIKVLRK--TCD-KGKDCRKANRNLGELGETSGGSILVREAVTVGPYE 322

Query: 264 ADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPKFKTNVMLIGDGINRTIIT 323
            DNF +I +A+A APN++ P+ GYFVIY R G YEEYVVI   K N+MLIGDGIN+TII+
Sbjct: 323 TDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIIS 382

Query: 324 GNHSVVDGWTTYNSS-------------------------------------TFTVCG-- 383
           GNHS +DGWTTYNSS                                     TF  C   
Sbjct: 383 GNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFE 442

Query: 384 ------------------DGFVAIDMTFRNTAG-----------PEKHQA------GRTD 443
                             D +  ID  F N A            P  +Q       GRTD
Sbjct: 443 GYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTD 502

Query: 444 PNQNTGISIHNCSIKAAPDWAMDWNATTMTTNYLGRPWKQYSRTVYMQSYIGNLISPVGW 490
           PNQ TGISI NC+I AAPD A D   +TMT  +LGRPWK YSRTVY+QSYI +++ PVGW
Sbjct: 503 PNQKTGISIINCTIGAAPDLAAD-PKSTMT--FLGRPWKPYSRTVYIQSYISDVVQPVGW 562

BLAST of ClCG05G022100 vs. Swiss-Prot
Match: PME25_ARATH (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana GN=PME25 PE=2 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 7.1e-97
Identity = 192/368 (52.17%), Postives = 250/368 (67.93%), Query Frame = 1

Query: 20  SDSSPPSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKKCLKQAIKMSSVISDYL 79
           S S  PS A CKST YPKLCR+IL++++ SPSDPY YGKF++K+CLKQA ++S VI+ Y 
Sbjct: 74  SQSQSPSLA-CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYA 133

Query: 80  DR--GRDSRLNQREAGALNDCRELSDLNVEFLRSIQGVLEAAEGVDEELVERVESILSAI 139
            R   +       E GA+ DC ELS+L+V +L ++   L+ A+ +   LVE V S+LS +
Sbjct: 134 RRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVEHVNSLLSGV 193

Query: 140 VTNGQTCIDGLVESRSSLGNALSAPLASAGELYSVSLGLVSNSLSRRWKK-RRQKGNGDG 199
           VTN QTC+DGLVE++S    A+ +P+ +   LYS+SLGLVS++L+R  K+ +  KG   G
Sbjct: 194 VTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILG 253

Query: 200 GNFPAGARSREPLNTLIKGLHKMGSCNNQSTKCSGRKRLLTDLGST---GILINNTVVVS 259
           G     +  REPL TLIKGL K  +C+N    C    R L +LG T    IL++  V+V 
Sbjct: 254 GG---NSTYREPLETLIKGLRK--TCDNDK-DCRKTSRNLGELGETSGGSILVSKAVIVG 313

Query: 260 STGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPKFKTNVMLIGDGINRT 319
              +DNFT+I DAIA APNN+ P+DGYFVIY REG YEEY+V+P  K N+ML+GDGIN+T
Sbjct: 314 PFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKT 373

Query: 320 IITGNHSVVDGWTTYNSSTFTVCGDGFVAIDMTFRNTAGPEKHQAGRTDPNQNTGISIHN 379
           IITGNH+VVDGWTTYN S+F V G+ F+A+D+TFRNTAGPEKHQA     N   G S + 
Sbjct: 374 IITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVAL-RNNAEGSSFYR 433

Query: 380 CSIKAAPD 382
           CS +   D
Sbjct: 434 CSFEGYQD 433


HSP 2 Score: 207.2 bits (526), Expect = 4.1e-52
Identity = 94/131 (71.76%), Postives = 109/131 (83.21%), Query Frame = 1

Query: 359 GRTDPNQNTGISIHNCSIKAAPDWAMDWNATTMTTNYLGRPWKQYSRTVYMQSYIGNLIS 418
           GR DPNQNTGISI NC+IKAAPD A +   + MT  +LGRPWK YSRTV+MQSYI +++ 
Sbjct: 486 GRLDPNQNTGISIINCTIKAAPDLAAE-PKSAMT--FLGRPWKPYSRTVFMQSYISDIVQ 545

Query: 419 PVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQAMNFTVYNFTMGDT 478
           PVGWLEWNGT+GL+T+YYGE+ N+GPGANT+ RV W GY+L+NL +AMNFTVYNFTMGDT
Sbjct: 546 PVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDT 605

Query: 479 WLPYTDIPFSG 490
           WLP TDIPF G
Sbjct: 606 WLPQTDIPFYG 613

BLAST of ClCG05G022100 vs. Swiss-Prot
Match: PME41_ARATH (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana GN=PME41 PE=2 SV=2)

HSP 1 Score: 226.9 bits (577), Expect = 5.0e-58
Identity = 148/391 (37.85%), Postives = 218/391 (55.75%), Query Frame = 1

Query: 6   FICLLLLSL-LLAGASDSSPPSTA---LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSV 65
           F+  L LSL  L  AS +  PS +   +CK+T  PK C+S+      S  D   YG+FS+
Sbjct: 7   FLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQT--SQGDVREYGRFSL 66

Query: 66  KKCLKQAIKMSSVISDYLDRGRDSRLNQREAGALNDCRELSDLNVEFLRS---IQGVLEA 125
           +K L Q+ K +  I  YL R  ++ L+Q   GAL DCR L+ L  ++L +      +  +
Sbjct: 67  RKSLTQSRKFTRTIDRYLKRN-NALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTS 126

Query: 126 AEGVDEELVERVESILSAIVTNGQTCIDGLVESRSS---LGNALSAPLASAGELYSVSLG 185
           ++ +     + ++++LSA +TN QTC+DG+  + SS   + N ++ PL +  +L+SVSL 
Sbjct: 127 SKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLA 186

Query: 186 LVSNSLSRRW--KKRRQKGNGDGGNFPAGARSREPLNTLIKGLH--KMGSCNNQSTKCSG 245
           L     ++ W  KK++Q  +    +        +P      G    KM        +   
Sbjct: 187 L----FTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLS 246

Query: 246 RKRLLTDLGSTG-ILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYY 305
           R++L  D      +L+++ V V+  G  NFT+I +A+  APN +    GYFVIYV  G Y
Sbjct: 247 RRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVY 306

Query: 306 EEYVVIPKFKTNVMLIGDGINRTIITGNHSVVDGWTTYNSSTFTVCGDGFVAIDMTFRNT 365
           EE VVI K K  +M+IGDGINRT++TGN +VVDGWTT+NS+TF V    FVA++MTFRNT
Sbjct: 307 EENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNT 366

Query: 366 AGPEKHQAGRTDPNQNTGISIHNCSIKAAPD 382
           AGPEKHQA     + +  I  ++CS +A  D
Sbjct: 367 AGPEKHQAVAMRSSADLSI-FYSCSFEAYQD 389


HSP 2 Score: 165.6 bits (418), Expect = 1.4e-39
Identity = 77/129 (59.69%), Postives = 91/129 (70.54%), Query Frame = 1

Query: 359 GRTDPNQNTGISIHNCSIKAAPDWAMDWNATTMTTNYLGRPWKQYSRTVYMQSYIGNLIS 418
           GRTDPNQNTGISIHNC+IK A D     N T  T  YLGRPWK+YSRTV+MQSYI  ++ 
Sbjct: 442 GRTDPNQNTGISIHNCTIKPADDLVSS-NYTVKT--YLGRPWKEYSRTVFMQSYIDEVVE 501

Query: 419 PVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQAMNFTVYNFTMGDT 478
           PVGW EWNG   L TLYY E+ N G G++T+ RV WPGY ++N T A NFTV NF +GD 
Sbjct: 502 PVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTVENFLLGDG 561

Query: 479 WLPYTDIPF 488
           W+  + +P+
Sbjct: 562 WMVQSGVPY 567

BLAST of ClCG05G022100 vs. Swiss-Prot
Match: PME20_ARATH (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana GN=PME20 PE=2 SV=2)

HSP 1 Score: 213.8 bits (543), Expect = 4.3e-54
Identity = 138/376 (36.70%), Postives = 202/376 (53.72%), Query Frame = 1

Query: 12  LSLLLAGASDSSPPSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKKCLKQAIKM 71
           +S  +    +++ PS  +C+    P  CRS+L +    P D Y YG+ S+++ L +A + 
Sbjct: 22  ISAQIPAIGNATSPSN-ICRFAPDPSYCRSVLPN---QPGDIYSYGRLSLRRSLSRARRF 81

Query: 72  SSVISDYLDRGRDSRLNQREAGALNDCRELSDLNVEFLRSIQGVLEAAEGVDEELVERVE 131
            S+I   LDR +     +   GAL DC+ L+ L +++L S     ++ + +     E V 
Sbjct: 82  ISMIDAELDR-KGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDVH 141

Query: 132 SILSAIVTNGQTCIDGLVESRSSLGNALSAPLASAGELYSVSLGLVSNSLSRRWKKRRQK 191
           + LSA +TN QTC++GL  + S   N LS  L +  +LY VSL L     S+ W  RRQ+
Sbjct: 142 TFLSAAITNEQTCLEGLKSTASE--NGLSGDLFNDTKLYGVSLAL----FSKGWVPRRQR 201

Query: 192 GN------GDGGNFPAGARSREPLNTLIKGLHKMGSCNNQSTKCSGRKRLLTDLGSTGIL 251
                        F      + PL    K   +  +  N  T+   RK L +D  +  + 
Sbjct: 202 SRPIWQPQARFKKFFGFRNGKLPL----KMTERARAVYNTVTR---RKLLQSD--ADAVQ 261

Query: 252 INNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPKFKTNVML 311
           +++ V V   G  NFT+I  AIA APN +   +GYF+IYV  G YEEYV +PK K  VM+
Sbjct: 262 VSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMM 321

Query: 312 IGDGINRTIITGNHSVVDGWTTYNSSTFTVCGDGFVAIDMTFRNTAGPEKHQAGRTDPNQ 371
           IGDGIN+T+ITGN SVVDGWTT+NS+TF + G  F+ +++T RNTAGP K QA       
Sbjct: 322 IGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGG 376

Query: 372 NTGISIHNCSIKAAPD 382
           +  +  ++CS +A  D
Sbjct: 382 DLSV-FYSCSFEAYQD 376


HSP 2 Score: 157.9 bits (398), Expect = 2.8e-37
Identity = 73/131 (55.73%), Postives = 88/131 (67.18%), Query Frame = 1

Query: 359 GRTDPNQNTGISIHNCSIKAAPDWAMDWNATTMTTNYLGRPWKQYSRTVYMQSYIGNLIS 418
           GRTDPNQNTG +IH C+I+ A D A   N T  T  YLGRPWK+YSRTV MQ+YI   + 
Sbjct: 429 GRTDPNQNTGTAIHGCTIRPADDLATS-NYTVKT--YLGRPWKEYSRTVVMQTYIDGFLE 488

Query: 419 PVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQAMNFTVYNFTMGDT 478
           P GW  W+G   L TLYY E+ N GPG++T+ RV WPGY ++N T A NFTV NF +G+ 
Sbjct: 489 PSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEG 548

Query: 479 WLPYTDIPFSG 490
           W+  T +PF G
Sbjct: 549 WIGQTGVPFVG 556

BLAST of ClCG05G022100 vs. Swiss-Prot
Match: PME7_ARATH (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana GN=PME7 PE=2 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 2.4e-52
Identity = 147/405 (36.30%), Postives = 217/405 (53.58%), Query Frame = 1

Query: 5   PFICLLLLSLLLAGASDSSPP---STALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSV 64
           P   L+ LS  L     SS     S+ +CK+T  PK C+S+      S  +   YG FS+
Sbjct: 4   PIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPH---SQGNVQQYGCFSI 63

Query: 65  KKCLKQAIKMSSVISDYLDRGRDSRLNQREA-GALNDCRELSDLNVEFL-RSIQGVLEAA 124
           +K L Q+ K    +  Y+ R  ++ L+Q     AL DCR L+ L +++L  S + V + +
Sbjct: 64  RKSLSQSRKFIRTVDRYIKR--NAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTS 123

Query: 125 -----EGVDEELVERVESILSAIVTNGQTCIDGLVESRS-----SLGNALSAPLASAGEL 184
                + +     + ++++LSA +TN QTC++GL  + S     ++   ++ PL +  +L
Sbjct: 124 AKTSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKL 183

Query: 185 YSVSLGLVSNSLSRRWKKRRQKGNGDGGNFPAGARSREPLNTLIKGLHKMGSCN---NQS 244
             VSL L     ++ W  +++K  G     P   RS    +T    L + G+      + 
Sbjct: 184 LGVSLAL----FTKGWVPKKKKRAGFAWAQP---RSGSSTHTKPFRLFRNGALPLKMTEK 243

Query: 245 TKC---SGRKRLLTDLGSTG-------ILINNTVVVSSTGADNFTSIGDAIAFAPNNSMP 304
           TK    S  +R L D  S G       +LI++ V VS  G  NFT+I  A+A APNN+  
Sbjct: 244 TKAVYESLSRRKLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDG 303

Query: 305 QDGYFVIYVREGYYEEYVVIPKFKTNVMLIGDGINRTIITGNHSVVDGWTTYNSSTFTVC 364
             G+F+IYV  G YEEY+ I K K  +M+IGDGIN+T++TGN SVVDGWTT+NS+TF V 
Sbjct: 304 SAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVT 363

Query: 365 GDGFVAIDMTFRNTAGPEKHQAGRTDPNQNTGISIHNCSIKAAPD 382
              FVA+++TFRNTAGPEKHQA       +  I  ++CS +A  D
Sbjct: 364 APNFVAVNITFRNTAGPEKHQAVALRSGADFSI-FYSCSFEAYQD 395


HSP 2 Score: 156.8 bits (395), Expect = 6.3e-37
Identity = 74/130 (56.92%), Postives = 86/130 (66.15%), Query Frame = 1

Query: 359 GRTDPNQNTGISIHNCSIKAAPDWAMDWNATTMTTNYLGRPWKQYSRTVYMQSYIGNLIS 418
           GR+DPNQNTG SI NC+IK A D     N T  T  YLGRPWK+YSRTVYMQSYI   + 
Sbjct: 448 GRSDPNQNTGTSIQNCTIKPADDLVSS-NYTVKT--YLGRPWKEYSRTVYMQSYIDGFVE 507

Query: 419 PVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQAMNFTVYNFTMGDT 478
           PVGW EWNG   L TLYY E+ N GPG+NT+ RV WPGY ++N T A NFTV    +   
Sbjct: 508 PVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEAD 567

Query: 479 WLPYTDIPFS 489
           W+  T +P++
Sbjct: 568 WIWKTGVPYT 574

BLAST of ClCG05G022100 vs. TrEMBL
Match: A0A0A0KUN8_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G612810 PE=4 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 1.1e-232
Identity = 422/563 (74.96%), Postives = 451/563 (80.11%), Query Frame = 1

Query: 1   MNSVPFICLLLLSLLLAGASDSSPPSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS+PFI LLLLSL  AGAS S PPSTALCKSTLYPKLCRSILS+IRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPPSTALCKSTLYPKLCRSILSTIRFSPSDPYGYGKFS 60

Query: 61  VKKCLKQAIKMSSVISDYLDRGRDSRLNQREAGALNDCRELSDLNVEFLRSIQGVLEAAE 120
           VK+C+KQA KMS+VI DYL+RGRDSRLN+ EAGAL+DCR+LSDLNVEFLRSI+ VLEAAE
Sbjct: 61  VKQCIKQATKMSTVIGDYLNRGRDSRLNRPEAGALSDCRDLSDLNVEFLRSIERVLEAAE 120

Query: 121 GVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLASAGELYSVSLGLVSNS 180
           GVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALS PL SAGELYSVSLGLVSN+
Sbjct: 121 GVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSGPLLSAGELYSVSLGLVSNA 180

Query: 181 LSRRWKKRRQKGNGDGGNFPAGARSREPLNTLIKGLHKMGSCNNQSTKCSGRKRLLTDLG 240
           +SRRWKKRR+KG G+GG  P G RSREPL+TLIKGLHKM  CNNQSTKC GR+RLLTDLG
Sbjct: 181 MSRRWKKRREKGGGNGG-VPGGGRSREPLDTLIKGLHKMEPCNNQSTKCLGRQRLLTDLG 240

Query: 241 STGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPKFK 300
           STGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYV+EGYYEEYVV+PKFK
Sbjct: 241 STGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVKEGYYEEYVVVPKFK 300

Query: 301 TNVMLIGDGINRTIITGNHSVVDGWTTYNSS----------------------------- 360
           TN+MLIGDGINRTIITGNH+VVDGWTTYNSS                             
Sbjct: 301 TNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 --------TFTVCG--------------------DGFVAIDMTFRNTAG----------- 420
                   TF  C                     D +  +D  F N A            
Sbjct: 361 LRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARK 420

Query: 421 --PEKHQA----GRTDPNQNTGISIHNCSIKAAPDWAMDWNATTMTTNYLGRPWKQYSRT 480
             P +  A    GRTDPNQNTGISIHNC+IKAAPDW MD N TT  TNYLGRPWKQYSRT
Sbjct: 421 PLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTT--TNYLGRPWKQYSRT 480

Query: 481 VYMQSYIGNLISPVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQAM 490
           VYMQSYIG+LISPVGWLEWNGTVGLETLYYGE+ENYGPGANTSLRVNWPG+SL+N+TQAM
Sbjct: 481 VYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLLNVTQAM 540

BLAST of ClCG05G022100 vs. TrEMBL
Match: B9RPV5_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_1541960 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 4.1e-160
Identity = 313/565 (55.40%), Postives = 373/565 (66.02%), Query Frame = 1

Query: 6   FICLLLLSLLLAGASDSSPPSTA-----LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 65
           F  LL LS  L  +S S P S +      CKS+LYPKLCRSILS+ R SPSD Y Y KFS
Sbjct: 7   FFALLFLSSALLISSQSPPQSQSQSPSLACKSSLYPKLCRSILSTYRSSPSDLYDYSKFS 66

Query: 66  VKKCLKQAIKMSSVISDYLDRGRD-SRLNQREAGALNDCRELSDLNVEFLRSIQGVLEAA 125
           VK+C+KQA ++S  I+ YL   +  S++N +E GAL DC EL+ LNV++L +I   L++A
Sbjct: 67  VKQCIKQANRLSKAINYYLTHDKHRSKINSKEIGALEDCHELTQLNVDYLGTISSELKSA 126

Query: 126 EGVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLASAGELYSVSLGLVSN 185
           E +++ELVERV S+LS IVTN QTC DGLVES+SS+   L APL +   LYSVSLGLV++
Sbjct: 127 ESMNDELVERVTSLLSGIVTNQQTCYDGLVESKSSIVAVLQAPLTNVTRLYSVSLGLVTH 186

Query: 186 SLSRRWKK-RRQKGNGDGGNFPAGARSREPLNTLIKGLHKMGSCNNQSTKCSGRKRLLTD 245
           +L R  KK +R K    G       R REPLNTLIK L K     +  ++C   +R L D
Sbjct: 187 ALDRNLKKNKRNKKGSHGKGILTKNRIREPLNTLIKALRKSSCHTSGGSRC---RRNLAD 246

Query: 246 LGSTGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPK 305
           +   GILIN+TV+VS  G DNFTSIGDAIA APNNS P+DGYFVIY REGYYEEYV++PK
Sbjct: 247 MEEDGILINDTVIVSPYGTDNFTSIGDAIAIAPNNSKPEDGYFVIYAREGYYEEYVIVPK 306

Query: 306 FKTNVMLIGDGINRTIITGNHSVVDGWTT------------------------------- 365
           +K N++LIGDGINRT+ITGNHSVVDGWTT                               
Sbjct: 307 YKKNILLIGDGINRTVITGNHSVVDGWTTFNSSTVAVSGERFVAVDVTFRNTAGPQKHQA 366

Query: 366 ------YNSSTFTVCG--------------------DGFVAIDMTFRNTAG--------- 425
                  + STF  C                     D +  +D  F N+A          
Sbjct: 367 VALRNNADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNSAAVFQSCNLYA 426

Query: 426 ----PEKHQA----GRTDPNQNTGISIHNCSIKAAPDWAMDWNATTMTTNYLGRPWKQYS 485
               P +  A    GRTDPNQNTGISIHNC+I+AAPD AMD N+T    N+LGRPWKQYS
Sbjct: 427 RKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIEAAPDLAMDLNSTL---NFLGRPWKQYS 486

Query: 486 RTVYMQSYIGNLISPVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQ 490
           RTV+MQSYIG+LISPVGWLEWNGTVGL+TLYYGEFENYGPGANTS+RV WPGY+LMN++Q
Sbjct: 487 RTVFMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSMRVQWPGYNLMNVSQ 546

BLAST of ClCG05G022100 vs. TrEMBL
Match: B9HVF5_POPTR (Pectinesterase OS=Populus trichocarpa GN=POPTR_0010s25400g PE=4 SV=2)

HSP 1 Score: 550.1 bits (1416), Expect = 2.8e-153
Identity = 297/563 (52.75%), Postives = 363/563 (64.48%), Query Frame = 1

Query: 6   FICLLLLSLLLAGASDSSPPSTA--LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKK 65
           F  L + S LL  +    PP +    CKSTLYPKLCRSIL++   S S+PY Y KFSVK+
Sbjct: 12  FAFLSIFSPLLISSQSPPPPYSPSNACKSTLYPKLCRSILTTFP-SSSNPYEYSKFSVKQ 71

Query: 66  CLKQAIKMSSVISDYLDRGRD-SRLNQREAGALNDCRELSDLNVEFLRSIQGVLEAAEGV 125
           CLKQA ++S VI  +L   +  S++   E GAL DC E  +LNV++  +I   L AAE +
Sbjct: 72  CLKQAKRLSKVIDYHLTHEKQLSKMTHEEFGALQDCHEFMELNVDYFETISSELVAAESM 131

Query: 126 DEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLASAGELYSVSLGLVSNSLS 185
            + LVERV S+LS +VTN QTC DGLV+S+SS+ +ALS PL++  +LYSVSL LV++SL 
Sbjct: 132 SDVLVERVTSLLSGVVTNQQTCYDGLVQSKSSIVSALSVPLSNVTQLYSVSLALVTHSLE 191

Query: 186 RRWKKRRQKGNGDGGNFPAGARSREPLNTLIKGLHKMGSCNNQSTKCSGRKRLLTDLGST 245
           +  KK +++     G    G R  EPL TLIK L K  SC+       G + L  D G  
Sbjct: 192 KNLKKNKRRKGSPQGTVTRGVR--EPLETLIKALRKTSSCHETRNCHRGERILSDDAGDD 251

Query: 246 GILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPKFKTN 305
           GIL+N+TV+V   G DNFT+IGDAIAFAPNNS P+DGYFVI+VREG YEEYVV+PK K N
Sbjct: 252 GILVNDTVIVGPYGTDNFTTIGDAIAFAPNNSKPEDGYFVIFVREGIYEEYVVVPKNKKN 311

Query: 306 VMLIGDGINRTIITGNHSVVDGWTTYNSS------------------------------- 365
           ++LIG+GIN+T+ITGNHSV+DGWTT+NSS                               
Sbjct: 312 IVLIGEGINQTVITGNHSVIDGWTTFNSSTFAVSGERFVGIDMTFRNTAGPEKHQAVALR 371

Query: 366 ------TFTVCG--------------------DGFVAIDMTFRNTAG-----------PE 425
                 TF  C                     D +  +D  F N A            P 
Sbjct: 372 NNADLSTFYRCSFEAYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQNCNLYARKPM 431

Query: 426 KHQA------GRTDPNQNTGISIHNCSIKAAPDWAMDWNAT--TMTTNYLGRPWKQYSRT 485
            +Q       GRTDPNQNTGISIHNC+I+AAPD AMD N+T   +T N+LGRPWK+YSRT
Sbjct: 432 LNQKNAFTAQGRTDPNQNTGISIHNCTIEAAPDLAMDRNSTDSNLTLNFLGRPWKEYSRT 491

Query: 486 VYMQSYIGNLISPVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQAM 490
           V MQSYIG LI PVGWLEWNGTVGL+T+YYGEF+NYGPGANTS RV WPG++LMN TQA+
Sbjct: 492 VIMQSYIGELIQPVGWLEWNGTVGLDTIYYGEFQNYGPGANTSRRVQWPGFNLMNATQAV 551

BLAST of ClCG05G022100 vs. TrEMBL
Match: A0A067K1K9_JATCU (Pectinesterase OS=Jatropha curcas GN=JCGZ_18594 PE=4 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 4.9e-153
Identity = 303/562 (53.91%), Postives = 366/562 (65.12%), Query Frame = 1

Query: 6   FICLLLLSLLLAGASDSSPPSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKKCL 65
           F  L L S LL  +  S  PS A CKST YPKLCRSILS+ RFS SDPY YGKFSVK+CL
Sbjct: 11  FTLLSLSSFLLVSSDQSQSPSIA-CKSTPYPKLCRSILSTFRFSSSDPYDYGKFSVKQCL 70

Query: 66  KQAIKMSSVISDYLDRGR-DSRLNQREAGALNDCRELSDLNVEFLRSIQGVLEAAEGVDE 125
           KQ  ++S  I+ YL   +  S+L++ E GAL DC EL+ L+V++L +I   L+AA  +  
Sbjct: 71  KQTHRLSKAINYYLTHDKYRSKLSREEIGALTDCHELTQLSVDYLETISTELQAAASLSG 130

Query: 126 ELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLASAGELYSVSLGLVSNSLSRR 185
           ELVERV S+LS +VTN  TC DGLV+S+SS+   L  P+A+  +LYSVSLGLV+++L R 
Sbjct: 131 ELVERVTSLLSGVVTNQDTCFDGLVDSKSSIAGGLYNPIANVSDLYSVSLGLVTHALDRN 190

Query: 186 WKKRRQKGNGDGGNFPAGARSREPLNTLIKGLHKMGSCNNQSTKCSGRKRL---LTDLGS 245
            KK ++  N        G R  EPL++LIK LHK  S  ++S     RK++   L D  S
Sbjct: 191 LKKNKRSKNQRSVFSKKGVR--EPLDSLIKALHKTKSSCHKSGGSDCRKKVGRNLADTES 250

Query: 246 TGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPKFKT 305
            GILIN+TV+VS  G  NFTSIGDAIA APNNS  +DGYFVIY REGYYEEYVV+PK+K 
Sbjct: 251 GGILINDTVIVSPYGDYNFTSIGDAIAIAPNNSKQEDGYFVIYAREGYYEEYVVVPKYKK 310

Query: 306 NVMLIGDGINRTIITGNHSVVDGWTTYN-------------------------------- 365
           N++LIGDGINRT+ITGNHSVVDGWTTYN                                
Sbjct: 311 NILLIGDGINRTVITGNHSVVDGWTTYNSSTFAVSGERFIAVDITFRNTAGPAKHQAVAV 370

Query: 366 -----SSTFTVCG--------------------DGFVAIDMTFRNTAG------------ 425
                 STF  C                     D +  +D  F N A             
Sbjct: 371 RNNADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQSCNLYTRKP 430

Query: 426 -PEKHQA----GRTDPNQNTGISIHNCSIKAAPDWAMDWNATTMTTNYLGRPWKQYSRTV 485
            P +  A    GRTDPNQNTGISIHNC+I+AAPD AM+ N++T+  NYLGRPWK+YSRTV
Sbjct: 431 LPNQKNAITAQGRTDPNQNTGISIHNCTIEAAPDLAMEVNSSTL--NYLGRPWKEYSRTV 490

Query: 486 YMQSYIGNLISPVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQAMN 490
           YM+SYIG+LI   GWL WNGTVGL+TLYYGEF+NYGPGANTSLRV W GYS+MN+++A+N
Sbjct: 491 YMESYIGDLIDKNGWLAWNGTVGLDTLYYGEFDNYGPGANTSLRVQWGGYSIMNVSEAVN 550

BLAST of ClCG05G022100 vs. TrEMBL
Match: B9HK85_POPTR (Pectinesterase OS=Populus trichocarpa GN=POPTR_0008s01180g PE=4 SV=2)

HSP 1 Score: 534.3 bits (1375), Expect = 1.6e-148
Identity = 295/571 (51.66%), Postives = 371/571 (64.97%), Query Frame = 1

Query: 7   ICLLLLSLL----------LAGASDSSPPSTALCKSTLYPKLCRSILSSIRFSPSDPYGY 66
           I LL++SLL          + G +  SP     CKSTLYPKLCRSIL++   S S+PY Y
Sbjct: 11  IVLLMISLLNLDSIREFKRVDGGAPQSPSDA--CKSTLYPKLCRSILTTFPSSSSNPYEY 70

Query: 67  GKFSVKKCLKQAIKMSSVISDYLD-RGRDSRLNQREAGALNDCRELSDLNVEFLRSIQGV 126
            KFSVK+C KQA ++S VI+ +L  + + S++   E GAL DC EL +LNV++  +I   
Sbjct: 71  SKFSVKQCHKQAKRLSKVINYHLTHKNQRSKMTHEEFGALQDCHELMELNVDYFETISSE 130

Query: 127 LEAAEGVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLASAGELYSVSLG 186
           L++AE +++ LVERV+S+LS +VTN Q+C DGLV+S+SS+ +ALS PL++   LYSVSL 
Sbjct: 131 LKSAESMNDVLVERVKSLLSGVVTNQQSCYDGLVQSKSSIASALSVPLSNGTRLYSVSLA 190

Query: 187 LVSNSLSRRWKKRR-QKGNGDGGNFPAGARSREPLNTLIKGLHKMGSCNNQSTKCSGRKR 246
           LV++SL +  KK + +KG+   G    G R  EPL TLIK L +  SC+ +S+ C   +R
Sbjct: 191 LVTHSLEKNLKKNKGRKGSHHHGILTKGVR--EPLETLIKALKRTASCH-KSSNCHRGER 250

Query: 247 LLTDLGSTGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYV 306
           +L+D    GIL+N++V+V   GADNFT+I DAIAFAPN+S P+DGYFVI+VREG YEEYV
Sbjct: 251 ILSDDSGDGILLNDSVIVGPYGADNFTTITDAIAFAPNSSTPEDGYFVIFVREGIYEEYV 310

Query: 307 VIPKFKTNVMLIGDGINRTIITGNHSVVDGWTTYNS------------------------ 366
           V+PK K N+M+IG+GINRTIITGNHSV+DGWTT+NS                        
Sbjct: 311 VVPKNKKNIMMIGEGINRTIITGNHSVMDGWTTFNSSTFAVSGERFVGVYITFRNTAGPQ 370

Query: 367 -------------STFTVCG--------------------DGFVAIDMTFRNTAG----- 426
                        STF  C                     D +  +D  F N A      
Sbjct: 371 KHQAVALRNNADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQNC 430

Query: 427 ------PEKHQA------GRTDPNQNTGISIHNCSIKAAPDWAMDWNAT--TMTTNYLGR 486
                 P  +Q       GRTDPNQNTGISI NC+I+AAPD AMD N+T    T+N+LGR
Sbjct: 431 NLYARKPMLNQKNAFTAQGRTDPNQNTGISIQNCTIEAAPDLAMDKNSTGSNSTSNFLGR 490

Query: 487 PWKQYSRTVYMQSYIGNLISPVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYS 490
           PWK YSRTV MQSYIG LI P GWLEWNGTVGL+T+YYGEF+NYGPG+NTS RV WPGY+
Sbjct: 491 PWKVYSRTVIMQSYIGELIQPAGWLEWNGTVGLDTIYYGEFQNYGPGSNTSRRVKWPGYN 550

BLAST of ClCG05G022100 vs. TAIR10
Match: AT5G04970.1 (AT5G04970.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 461.8 bits (1187), Expect = 5.2e-130
Identity = 267/547 (48.81%), Postives = 332/547 (60.69%), Query Frame = 1

Query: 24  PPSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKKCLKQAIKMSSVISDYLDR-- 83
           PP+ A CKST YPKLCR+ILS+++ SPSDPY YGKF++K+CLKQA ++S VI+ +  R  
Sbjct: 83  PPNIA-CKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQRVE 142

Query: 84  GRDSRLNQREAGALNDCRELSDLNVEFLRSIQGVLEAAEGVDEELVERVESILSAIVTNG 143
                    E  A+ DC EL++L+VE+L ++   L+AAE +   LV+RV S+L  +VTN 
Sbjct: 143 ADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAALVDRVTSLLGGVVTNQ 202

Query: 144 QTCIDGLVESRSSLGNALSAPLASAGELYSVSLGLVSNSLSRRWKKRR-QKGN-GDGGNF 203
           QTC+DGLV+++S    A+  PL +   LYSVSLGLVS++L+R  K+ +  KG    GGN 
Sbjct: 203 QTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSKGKIFGGGNK 262

Query: 204 PAGARSREPLNTLIKGLHKMGSCNNQSTKCSGRKRLLTDLGSTG---ILINNTVVVSSTG 263
           P     REPL TLIK L K  +C+ +   C    R L +LG T    IL+   V V    
Sbjct: 263 PV----REPLETLIKVLRK--TCD-KGKDCRKANRNLGELGETSGGSILVREAVTVGPYE 322

Query: 264 ADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPKFKTNVMLIGDGINRTIIT 323
            DNF +I +A+A APN++ P+ GYFVIY R G YEEYVVI   K N+MLIGDGIN+TII+
Sbjct: 323 TDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIIS 382

Query: 324 GNHSVVDGWTTYNSS-------------------------------------TFTVCG-- 383
           GNHS +DGWTTYNSS                                     TF  C   
Sbjct: 383 GNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFE 442

Query: 384 ------------------DGFVAIDMTFRNTAG-----------PEKHQA------GRTD 443
                             D +  ID  F N A            P  +Q       GRTD
Sbjct: 443 GYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTD 502

Query: 444 PNQNTGISIHNCSIKAAPDWAMDWNATTMTTNYLGRPWKQYSRTVYMQSYIGNLISPVGW 490
           PNQ TGISI NC+I AAPD A D   +TMT  +LGRPWK YSRTVY+QSYI +++ PVGW
Sbjct: 503 PNQKTGISIINCTIGAAPDLAAD-PKSTMT--FLGRPWKPYSRTVYIQSYISDVVQPVGW 562

BLAST of ClCG05G022100 vs. TAIR10
Match: AT3G10720.2 (AT3G10720.2 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 355.9 bits (912), Expect = 4.0e-98
Identity = 192/368 (52.17%), Postives = 250/368 (67.93%), Query Frame = 1

Query: 20  SDSSPPSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKKCLKQAIKMSSVISDYL 79
           S S  PS A CKST YPKLCR+IL++++ SPSDPY YGKF++K+CLKQA ++S VI+ Y 
Sbjct: 74  SQSQSPSLA-CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYA 133

Query: 80  DR--GRDSRLNQREAGALNDCRELSDLNVEFLRSIQGVLEAAEGVDEELVERVESILSAI 139
            R   +       E GA+ DC ELS+L+V +L ++   L+ A+ +   LVE V S+LS +
Sbjct: 134 RRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVEHVNSLLSGV 193

Query: 140 VTNGQTCIDGLVESRSSLGNALSAPLASAGELYSVSLGLVSNSLSRRWKK-RRQKGNGDG 199
           VTN QTC+DGLVE++S    A+ +P+ +   LYS+SLGLVS++L+R  K+ +  KG   G
Sbjct: 194 VTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILG 253

Query: 200 GNFPAGARSREPLNTLIKGLHKMGSCNNQSTKCSGRKRLLTDLGST---GILINNTVVVS 259
           G     +  REPL TLIKGL K  +C+N    C    R L +LG T    IL++  V+V 
Sbjct: 254 GG---NSTYREPLETLIKGLRK--TCDNDK-DCRKTSRNLGELGETSGGSILVSKAVIVG 313

Query: 260 STGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPKFKTNVMLIGDGINRT 319
              +DNFT+I DAIA APNN+ P+DGYFVIY REG YEEY+V+P  K N+ML+GDGIN+T
Sbjct: 314 PFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKT 373

Query: 320 IITGNHSVVDGWTTYNSSTFTVCGDGFVAIDMTFRNTAGPEKHQAGRTDPNQNTGISIHN 379
           IITGNH+VVDGWTTYN S+F V G+ F+A+D+TFRNTAGPEKHQA     N   G S + 
Sbjct: 374 IITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVAL-RNNAEGSSFYR 433

Query: 380 CSIKAAPD 382
           CS +   D
Sbjct: 434 CSFEGYQD 433


HSP 2 Score: 207.2 bits (526), Expect = 2.3e-53
Identity = 94/131 (71.76%), Postives = 109/131 (83.21%), Query Frame = 1

Query: 359 GRTDPNQNTGISIHNCSIKAAPDWAMDWNATTMTTNYLGRPWKQYSRTVYMQSYIGNLIS 418
           GR DPNQNTGISI NC+IKAAPD A +   + MT  +LGRPWK YSRTV+MQSYI +++ 
Sbjct: 486 GRLDPNQNTGISIINCTIKAAPDLAAE-PKSAMT--FLGRPWKPYSRTVFMQSYISDIVQ 545

Query: 419 PVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQAMNFTVYNFTMGDT 478
           PVGWLEWNGT+GL+T+YYGE+ N+GPGANT+ RV W GY+L+NL +AMNFTVYNFTMGDT
Sbjct: 546 PVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDT 605

Query: 479 WLPYTDIPFSG 490
           WLP TDIPF G
Sbjct: 606 WLPQTDIPFYG 613

BLAST of ClCG05G022100 vs. TAIR10
Match: AT4G02330.1 (AT4G02330.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 226.9 bits (577), Expect = 2.8e-59
Identity = 148/391 (37.85%), Postives = 218/391 (55.75%), Query Frame = 1

Query: 6   FICLLLLSL-LLAGASDSSPPSTA---LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSV 65
           F+  L LSL  L  AS +  PS +   +CK+T  PK C+S+      S  D   YG+FS+
Sbjct: 7   FLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQT--SQGDVREYGRFSL 66

Query: 66  KKCLKQAIKMSSVISDYLDRGRDSRLNQREAGALNDCRELSDLNVEFLRS---IQGVLEA 125
           +K L Q+ K +  I  YL R  ++ L+Q   GAL DCR L+ L  ++L +      +  +
Sbjct: 67  RKSLTQSRKFTRTIDRYLKRN-NALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTS 126

Query: 126 AEGVDEELVERVESILSAIVTNGQTCIDGLVESRSS---LGNALSAPLASAGELYSVSLG 185
           ++ +     + ++++LSA +TN QTC+DG+  + SS   + N ++ PL +  +L+SVSL 
Sbjct: 127 SKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLA 186

Query: 186 LVSNSLSRRW--KKRRQKGNGDGGNFPAGARSREPLNTLIKGLH--KMGSCNNQSTKCSG 245
           L     ++ W  KK++Q  +    +        +P      G    KM        +   
Sbjct: 187 L----FTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLS 246

Query: 246 RKRLLTDLGSTG-ILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYY 305
           R++L  D      +L+++ V V+  G  NFT+I +A+  APN +    GYFVIYV  G Y
Sbjct: 247 RRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVY 306

Query: 306 EEYVVIPKFKTNVMLIGDGINRTIITGNHSVVDGWTTYNSSTFTVCGDGFVAIDMTFRNT 365
           EE VVI K K  +M+IGDGINRT++TGN +VVDGWTT+NS+TF V    FVA++MTFRNT
Sbjct: 307 EENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNT 366

Query: 366 AGPEKHQAGRTDPNQNTGISIHNCSIKAAPD 382
           AGPEKHQA     + +  I  ++CS +A  D
Sbjct: 367 AGPEKHQAVAMRSSADLSI-FYSCSFEAYQD 389


HSP 2 Score: 165.6 bits (418), Expect = 7.6e-41
Identity = 77/129 (59.69%), Postives = 91/129 (70.54%), Query Frame = 1

Query: 359 GRTDPNQNTGISIHNCSIKAAPDWAMDWNATTMTTNYLGRPWKQYSRTVYMQSYIGNLIS 418
           GRTDPNQNTGISIHNC+IK A D     N T  T  YLGRPWK+YSRTV+MQSYI  ++ 
Sbjct: 442 GRTDPNQNTGISIHNCTIKPADDLVSS-NYTVKT--YLGRPWKEYSRTVFMQSYIDEVVE 501

Query: 419 PVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQAMNFTVYNFTMGDT 478
           PVGW EWNG   L TLYY E+ N G G++T+ RV WPGY ++N T A NFTV NF +GD 
Sbjct: 502 PVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTVENFLLGDG 561

Query: 479 WLPYTDIPF 488
           W+  + +P+
Sbjct: 562 WMVQSGVPY 567

BLAST of ClCG05G022100 vs. TAIR10
Match: AT2G47550.1 (AT2G47550.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 213.8 bits (543), Expect = 2.4e-55
Identity = 138/376 (36.70%), Postives = 202/376 (53.72%), Query Frame = 1

Query: 12  LSLLLAGASDSSPPSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKKCLKQAIKM 71
           +S  +    +++ PS  +C+    P  CRS+L +    P D Y YG+ S+++ L +A + 
Sbjct: 22  ISAQIPAIGNATSPSN-ICRFAPDPSYCRSVLPN---QPGDIYSYGRLSLRRSLSRARRF 81

Query: 72  SSVISDYLDRGRDSRLNQREAGALNDCRELSDLNVEFLRSIQGVLEAAEGVDEELVERVE 131
            S+I   LDR +     +   GAL DC+ L+ L +++L S     ++ + +     E V 
Sbjct: 82  ISMIDAELDR-KGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDVH 141

Query: 132 SILSAIVTNGQTCIDGLVESRSSLGNALSAPLASAGELYSVSLGLVSNSLSRRWKKRRQK 191
           + LSA +TN QTC++GL  + S   N LS  L +  +LY VSL L     S+ W  RRQ+
Sbjct: 142 TFLSAAITNEQTCLEGLKSTASE--NGLSGDLFNDTKLYGVSLAL----FSKGWVPRRQR 201

Query: 192 GN------GDGGNFPAGARSREPLNTLIKGLHKMGSCNNQSTKCSGRKRLLTDLGSTGIL 251
                        F      + PL    K   +  +  N  T+   RK L +D  +  + 
Sbjct: 202 SRPIWQPQARFKKFFGFRNGKLPL----KMTERARAVYNTVTR---RKLLQSD--ADAVQ 261

Query: 252 INNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPKFKTNVML 311
           +++ V V   G  NFT+I  AIA APN +   +GYF+IYV  G YEEYV +PK K  VM+
Sbjct: 262 VSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMM 321

Query: 312 IGDGINRTIITGNHSVVDGWTTYNSSTFTVCGDGFVAIDMTFRNTAGPEKHQAGRTDPNQ 371
           IGDGIN+T+ITGN SVVDGWTT+NS+TF + G  F+ +++T RNTAGP K QA       
Sbjct: 322 IGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGG 376

Query: 372 NTGISIHNCSIKAAPD 382
           +  +  ++CS +A  D
Sbjct: 382 DLSV-FYSCSFEAYQD 376


HSP 2 Score: 157.9 bits (398), Expect = 1.6e-38
Identity = 73/131 (55.73%), Postives = 88/131 (67.18%), Query Frame = 1

Query: 359 GRTDPNQNTGISIHNCSIKAAPDWAMDWNATTMTTNYLGRPWKQYSRTVYMQSYIGNLIS 418
           GRTDPNQNTG +IH C+I+ A D A   N T  T  YLGRPWK+YSRTV MQ+YI   + 
Sbjct: 429 GRTDPNQNTGTAIHGCTIRPADDLATS-NYTVKT--YLGRPWKEYSRTVVMQTYIDGFLE 488

Query: 419 PVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQAMNFTVYNFTMGDT 478
           P GW  W+G   L TLYY E+ N GPG++T+ RV WPGY ++N T A NFTV NF +G+ 
Sbjct: 489 PSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEG 548

Query: 479 WLPYTDIPFSG 490
           W+  T +PF G
Sbjct: 549 WIGQTGVPFVG 556

BLAST of ClCG05G022100 vs. TAIR10
Match: AT1G02810.1 (AT1G02810.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 208.0 bits (528), Expect = 1.3e-53
Identity = 147/405 (36.30%), Postives = 217/405 (53.58%), Query Frame = 1

Query: 5   PFICLLLLSLLLAGASDSSPP---STALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSV 64
           P   L+ LS  L     SS     S+ +CK+T  PK C+S+      S  +   YG FS+
Sbjct: 4   PIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPH---SQGNVQQYGCFSI 63

Query: 65  KKCLKQAIKMSSVISDYLDRGRDSRLNQREA-GALNDCRELSDLNVEFL-RSIQGVLEAA 124
           +K L Q+ K    +  Y+ R  ++ L+Q     AL DCR L+ L +++L  S + V + +
Sbjct: 64  RKSLSQSRKFIRTVDRYIKR--NAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTS 123

Query: 125 -----EGVDEELVERVESILSAIVTNGQTCIDGLVESRS-----SLGNALSAPLASAGEL 184
                + +     + ++++LSA +TN QTC++GL  + S     ++   ++ PL +  +L
Sbjct: 124 AKTSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKL 183

Query: 185 YSVSLGLVSNSLSRRWKKRRQKGNGDGGNFPAGARSREPLNTLIKGLHKMGSCN---NQS 244
             VSL L     ++ W  +++K  G     P   RS    +T    L + G+      + 
Sbjct: 184 LGVSLAL----FTKGWVPKKKKRAGFAWAQP---RSGSSTHTKPFRLFRNGALPLKMTEK 243

Query: 245 TKC---SGRKRLLTDLGSTG-------ILINNTVVVSSTGADNFTSIGDAIAFAPNNSMP 304
           TK    S  +R L D  S G       +LI++ V VS  G  NFT+I  A+A APNN+  
Sbjct: 244 TKAVYESLSRRKLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDG 303

Query: 305 QDGYFVIYVREGYYEEYVVIPKFKTNVMLIGDGINRTIITGNHSVVDGWTTYNSSTFTVC 364
             G+F+IYV  G YEEY+ I K K  +M+IGDGIN+T++TGN SVVDGWTT+NS+TF V 
Sbjct: 304 SAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVT 363

Query: 365 GDGFVAIDMTFRNTAGPEKHQAGRTDPNQNTGISIHNCSIKAAPD 382
              FVA+++TFRNTAGPEKHQA       +  I  ++CS +A  D
Sbjct: 364 APNFVAVNITFRNTAGPEKHQAVALRSGADFSI-FYSCSFEAYQD 395


HSP 2 Score: 156.8 bits (395), Expect = 3.5e-38
Identity = 74/130 (56.92%), Postives = 86/130 (66.15%), Query Frame = 1

Query: 359 GRTDPNQNTGISIHNCSIKAAPDWAMDWNATTMTTNYLGRPWKQYSRTVYMQSYIGNLIS 418
           GR+DPNQNTG SI NC+IK A D     N T  T  YLGRPWK+YSRTVYMQSYI   + 
Sbjct: 448 GRSDPNQNTGTSIQNCTIKPADDLVSS-NYTVKT--YLGRPWKEYSRTVYMQSYIDGFVE 507

Query: 419 PVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQAMNFTVYNFTMGDT 478
           PVGW EWNG   L TLYY E+ N GPG+NT+ RV WPGY ++N T A NFTV    +   
Sbjct: 508 PVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEAD 567

Query: 479 WLPYTDIPFS 489
           W+  T +P++
Sbjct: 568 WIWKTGVPYT 574

BLAST of ClCG05G022100 vs. NCBI nr
Match: gi|659091753|ref|XP_008446714.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Cucumis melo])

HSP 1 Score: 820.1 bits (2117), Expect = 2.1e-234
Identity = 427/563 (75.84%), Postives = 450/563 (79.93%), Query Frame = 1

Query: 1   MNSVPFICLLLLSLLLAGASDSSPPSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS+PFI LLLLSL  AGAS S P STALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKKCLKQAIKMSSVISDYLDRGRDSRLNQREAGALNDCRELSDLNVEFLRSIQGVLEAAE 120
           VK+C+KQAIKMS+VI DYLDRGRDSRLN+REAGAL+DCRELSDLNVEFLRSI+ VLEAAE
Sbjct: 61  VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 120

Query: 121 GVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLASAGELYSVSLGLVSNS 180
           GVDEELVERVES LSAIVTNGQTC+DGLVESRSSLG ALSAPL SAGELYSVSLGLVSN+
Sbjct: 121 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 180

Query: 181 LSRRWKKRRQKGNGDGGNFPAGARSREPLNTLIKGLHKMGSCNNQSTKCSGRKRLLTDLG 240
           +SRRWKKRR+KG G+GG  P G RSRE LNTLIKGLHKM  CNN STKCSGRKRLLTDLG
Sbjct: 181 MSRRWKKRREKGGGNGG-VPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLG 240

Query: 241 STGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPKFK 300
           S+GILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVV+PKFK
Sbjct: 241 SSGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFK 300

Query: 301 TNVMLIGDGINRTIITGNHSVVDGWTTYNSS----------------------------- 360
           TN+MLIGDGINRTIITGNH+VVDGWTTYNSS                             
Sbjct: 301 TNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 --------TFTVCG--------------------DGFVAIDMTFRNTAG----------- 420
                   TF  C                     D +  +D  F N A            
Sbjct: 361 LRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARK 420

Query: 421 --PEKHQA----GRTDPNQNTGISIHNCSIKAAPDWAMDWNATTMTTNYLGRPWKQYSRT 480
             P +  A    GRTDPNQNTGISIHNC+IKAAPDW MD N TT  TNYLGRPWKQYSRT
Sbjct: 421 PLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTT--TNYLGRPWKQYSRT 480

Query: 481 VYMQSYIGNLISPVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQAM 490
           VYMQSYIG+LISPVGWLEWNGTVGLETLYYGE+ENYGPGANTSLRVNWPG+SLMNLTQAM
Sbjct: 481 VYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLMNLTQAM 540

BLAST of ClCG05G022100 vs. NCBI nr
Match: gi|449435184|ref|XP_004135375.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Cucumis sativus])

HSP 1 Score: 813.9 bits (2101), Expect = 1.5e-232
Identity = 422/563 (74.96%), Postives = 451/563 (80.11%), Query Frame = 1

Query: 1   MNSVPFICLLLLSLLLAGASDSSPPSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS+PFI LLLLSL  AGAS S PPSTALCKSTLYPKLCRSILS+IRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPPSTALCKSTLYPKLCRSILSTIRFSPSDPYGYGKFS 60

Query: 61  VKKCLKQAIKMSSVISDYLDRGRDSRLNQREAGALNDCRELSDLNVEFLRSIQGVLEAAE 120
           VK+C+KQA KMS+VI DYL+RGRDSRLN+ EAGAL+DCR+LSDLNVEFLRSI+ VLEAAE
Sbjct: 61  VKQCIKQATKMSTVIGDYLNRGRDSRLNRPEAGALSDCRDLSDLNVEFLRSIERVLEAAE 120

Query: 121 GVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLASAGELYSVSLGLVSNS 180
           GVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALS PL SAGELYSVSLGLVSN+
Sbjct: 121 GVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSGPLLSAGELYSVSLGLVSNA 180

Query: 181 LSRRWKKRRQKGNGDGGNFPAGARSREPLNTLIKGLHKMGSCNNQSTKCSGRKRLLTDLG 240
           +SRRWKKRR+KG G+GG  P G RSREPL+TLIKGLHKM  CNNQSTKC GR+RLLTDLG
Sbjct: 181 MSRRWKKRREKGGGNGG-VPGGGRSREPLDTLIKGLHKMEPCNNQSTKCLGRQRLLTDLG 240

Query: 241 STGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPKFK 300
           STGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYV+EGYYEEYVV+PKFK
Sbjct: 241 STGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVKEGYYEEYVVVPKFK 300

Query: 301 TNVMLIGDGINRTIITGNHSVVDGWTTYNSS----------------------------- 360
           TN+MLIGDGINRTIITGNH+VVDGWTTYNSS                             
Sbjct: 301 TNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 --------TFTVCG--------------------DGFVAIDMTFRNTAG----------- 420
                   TF  C                     D +  +D  F N A            
Sbjct: 361 LRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARK 420

Query: 421 --PEKHQA----GRTDPNQNTGISIHNCSIKAAPDWAMDWNATTMTTNYLGRPWKQYSRT 480
             P +  A    GRTDPNQNTGISIHNC+IKAAPDW MD N TT  TNYLGRPWKQYSRT
Sbjct: 421 PLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTT--TNYLGRPWKQYSRT 480

Query: 481 VYMQSYIGNLISPVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQAM 490
           VYMQSYIG+LISPVGWLEWNGTVGLETLYYGE+ENYGPGANTSLRVNWPG+SL+N+TQAM
Sbjct: 481 VYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLLNVTQAM 540

BLAST of ClCG05G022100 vs. NCBI nr
Match: gi|255549442|ref|XP_002515774.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Ricinus communis])

HSP 1 Score: 572.8 bits (1475), Expect = 5.9e-160
Identity = 313/565 (55.40%), Postives = 373/565 (66.02%), Query Frame = 1

Query: 6   FICLLLLSLLLAGASDSSPPSTA-----LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 65
           F  LL LS  L  +S S P S +      CKS+LYPKLCRSILS+ R SPSD Y Y KFS
Sbjct: 7   FFALLFLSSALLISSQSPPQSQSQSPSLACKSSLYPKLCRSILSTYRSSPSDLYDYSKFS 66

Query: 66  VKKCLKQAIKMSSVISDYLDRGRD-SRLNQREAGALNDCRELSDLNVEFLRSIQGVLEAA 125
           VK+C+KQA ++S  I+ YL   +  S++N +E GAL DC EL+ LNV++L +I   L++A
Sbjct: 67  VKQCIKQANRLSKAINYYLTHDKHRSKINSKEIGALEDCHELTQLNVDYLGTISSELKSA 126

Query: 126 EGVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLASAGELYSVSLGLVSN 185
           E +++ELVERV S+LS IVTN QTC DGLVES+SS+   L APL +   LYSVSLGLV++
Sbjct: 127 ESMNDELVERVTSLLSGIVTNQQTCYDGLVESKSSIVAVLQAPLTNVTRLYSVSLGLVTH 186

Query: 186 SLSRRWKK-RRQKGNGDGGNFPAGARSREPLNTLIKGLHKMGSCNNQSTKCSGRKRLLTD 245
           +L R  KK +R K    G       R REPLNTLIK L K     +  ++C   +R L D
Sbjct: 187 ALDRNLKKNKRNKKGSHGKGILTKNRIREPLNTLIKALRKSSCHTSGGSRC---RRNLAD 246

Query: 246 LGSTGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPK 305
           +   GILIN+TV+VS  G DNFTSIGDAIA APNNS P+DGYFVIY REGYYEEYV++PK
Sbjct: 247 MEEDGILINDTVIVSPYGTDNFTSIGDAIAIAPNNSKPEDGYFVIYAREGYYEEYVIVPK 306

Query: 306 FKTNVMLIGDGINRTIITGNHSVVDGWTT------------------------------- 365
           +K N++LIGDGINRT+ITGNHSVVDGWTT                               
Sbjct: 307 YKKNILLIGDGINRTVITGNHSVVDGWTTFNSSTVAVSGERFVAVDVTFRNTAGPQKHQA 366

Query: 366 ------YNSSTFTVCG--------------------DGFVAIDMTFRNTAG--------- 425
                  + STF  C                     D +  +D  F N+A          
Sbjct: 367 VALRNNADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNSAAVFQSCNLYA 426

Query: 426 ----PEKHQA----GRTDPNQNTGISIHNCSIKAAPDWAMDWNATTMTTNYLGRPWKQYS 485
               P +  A    GRTDPNQNTGISIHNC+I+AAPD AMD N+T    N+LGRPWKQYS
Sbjct: 427 RKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIEAAPDLAMDLNSTL---NFLGRPWKQYS 486

Query: 486 RTVYMQSYIGNLISPVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQ 490
           RTV+MQSYIG+LISPVGWLEWNGTVGL+TLYYGEFENYGPGANTS+RV WPGY+LMN++Q
Sbjct: 487 RTVFMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSMRVQWPGYNLMNVSQ 546

BLAST of ClCG05G022100 vs. NCBI nr
Match: gi|566192712|ref|XP_002315399.2| (hypothetical protein POPTR_0010s25400g [Populus trichocarpa])

HSP 1 Score: 550.1 bits (1416), Expect = 4.1e-153
Identity = 297/563 (52.75%), Postives = 363/563 (64.48%), Query Frame = 1

Query: 6   FICLLLLSLLLAGASDSSPPSTA--LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKK 65
           F  L + S LL  +    PP +    CKSTLYPKLCRSIL++   S S+PY Y KFSVK+
Sbjct: 12  FAFLSIFSPLLISSQSPPPPYSPSNACKSTLYPKLCRSILTTFP-SSSNPYEYSKFSVKQ 71

Query: 66  CLKQAIKMSSVISDYLDRGRD-SRLNQREAGALNDCRELSDLNVEFLRSIQGVLEAAEGV 125
           CLKQA ++S VI  +L   +  S++   E GAL DC E  +LNV++  +I   L AAE +
Sbjct: 72  CLKQAKRLSKVIDYHLTHEKQLSKMTHEEFGALQDCHEFMELNVDYFETISSELVAAESM 131

Query: 126 DEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLASAGELYSVSLGLVSNSLS 185
            + LVERV S+LS +VTN QTC DGLV+S+SS+ +ALS PL++  +LYSVSL LV++SL 
Sbjct: 132 SDVLVERVTSLLSGVVTNQQTCYDGLVQSKSSIVSALSVPLSNVTQLYSVSLALVTHSLE 191

Query: 186 RRWKKRRQKGNGDGGNFPAGARSREPLNTLIKGLHKMGSCNNQSTKCSGRKRLLTDLGST 245
           +  KK +++     G    G R  EPL TLIK L K  SC+       G + L  D G  
Sbjct: 192 KNLKKNKRRKGSPQGTVTRGVR--EPLETLIKALRKTSSCHETRNCHRGERILSDDAGDD 251

Query: 246 GILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPKFKTN 305
           GIL+N+TV+V   G DNFT+IGDAIAFAPNNS P+DGYFVI+VREG YEEYVV+PK K N
Sbjct: 252 GILVNDTVIVGPYGTDNFTTIGDAIAFAPNNSKPEDGYFVIFVREGIYEEYVVVPKNKKN 311

Query: 306 VMLIGDGINRTIITGNHSVVDGWTTYNSS------------------------------- 365
           ++LIG+GIN+T+ITGNHSV+DGWTT+NSS                               
Sbjct: 312 IVLIGEGINQTVITGNHSVIDGWTTFNSSTFAVSGERFVGIDMTFRNTAGPEKHQAVALR 371

Query: 366 ------TFTVCG--------------------DGFVAIDMTFRNTAG-----------PE 425
                 TF  C                     D +  +D  F N A            P 
Sbjct: 372 NNADLSTFYRCSFEAYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQNCNLYARKPM 431

Query: 426 KHQA------GRTDPNQNTGISIHNCSIKAAPDWAMDWNAT--TMTTNYLGRPWKQYSRT 485
            +Q       GRTDPNQNTGISIHNC+I+AAPD AMD N+T   +T N+LGRPWK+YSRT
Sbjct: 432 LNQKNAFTAQGRTDPNQNTGISIHNCTIEAAPDLAMDRNSTDSNLTLNFLGRPWKEYSRT 491

Query: 486 VYMQSYIGNLISPVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQAM 490
           V MQSYIG LI PVGWLEWNGTVGL+T+YYGEF+NYGPGANTS RV WPG++LMN TQA+
Sbjct: 492 VIMQSYIGELIQPVGWLEWNGTVGLDTIYYGEFQNYGPGANTSRRVQWPGFNLMNATQAV 551

BLAST of ClCG05G022100 vs. NCBI nr
Match: gi|802667492|ref|XP_012081346.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Jatropha curcas])

HSP 1 Score: 549.3 bits (1414), Expect = 7.0e-153
Identity = 303/562 (53.91%), Postives = 366/562 (65.12%), Query Frame = 1

Query: 6   FICLLLLSLLLAGASDSSPPSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKKCL 65
           F  L L S LL  +  S  PS A CKST YPKLCRSILS+ RFS SDPY YGKFSVK+CL
Sbjct: 11  FTLLSLSSFLLVSSDQSQSPSIA-CKSTPYPKLCRSILSTFRFSSSDPYDYGKFSVKQCL 70

Query: 66  KQAIKMSSVISDYLDRGR-DSRLNQREAGALNDCRELSDLNVEFLRSIQGVLEAAEGVDE 125
           KQ  ++S  I+ YL   +  S+L++ E GAL DC EL+ L+V++L +I   L+AA  +  
Sbjct: 71  KQTHRLSKAINYYLTHDKYRSKLSREEIGALTDCHELTQLSVDYLETISTELQAAASLSG 130

Query: 126 ELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSAPLASAGELYSVSLGLVSNSLSRR 185
           ELVERV S+LS +VTN  TC DGLV+S+SS+   L  P+A+  +LYSVSLGLV+++L R 
Sbjct: 131 ELVERVTSLLSGVVTNQDTCFDGLVDSKSSIAGGLYNPIANVSDLYSVSLGLVTHALDRN 190

Query: 186 WKKRRQKGNGDGGNFPAGARSREPLNTLIKGLHKMGSCNNQSTKCSGRKRL---LTDLGS 245
            KK ++  N        G R  EPL++LIK LHK  S  ++S     RK++   L D  S
Sbjct: 191 LKKNKRSKNQRSVFSKKGVR--EPLDSLIKALHKTKSSCHKSGGSDCRKKVGRNLADTES 250

Query: 246 TGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPKFKT 305
            GILIN+TV+VS  G  NFTSIGDAIA APNNS  +DGYFVIY REGYYEEYVV+PK+K 
Sbjct: 251 GGILINDTVIVSPYGDYNFTSIGDAIAIAPNNSKQEDGYFVIYAREGYYEEYVVVPKYKK 310

Query: 306 NVMLIGDGINRTIITGNHSVVDGWTTYN-------------------------------- 365
           N++LIGDGINRT+ITGNHSVVDGWTTYN                                
Sbjct: 311 NILLIGDGINRTVITGNHSVVDGWTTYNSSTFAVSGERFIAVDITFRNTAGPAKHQAVAV 370

Query: 366 -----SSTFTVCG--------------------DGFVAIDMTFRNTAG------------ 425
                 STF  C                     D +  +D  F N A             
Sbjct: 371 RNNADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQSCNLYTRKP 430

Query: 426 -PEKHQA----GRTDPNQNTGISIHNCSIKAAPDWAMDWNATTMTTNYLGRPWKQYSRTV 485
            P +  A    GRTDPNQNTGISIHNC+I+AAPD AM+ N++T+  NYLGRPWK+YSRTV
Sbjct: 431 LPNQKNAITAQGRTDPNQNTGISIHNCTIEAAPDLAMEVNSSTL--NYLGRPWKEYSRTV 490

Query: 486 YMQSYIGNLISPVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQAMN 490
           YM+SYIG+LI   GWL WNGTVGL+TLYYGEF+NYGPGANTSLRV W GYS+MN+++A+N
Sbjct: 491 YMESYIGDLIDKNGWLAWNGTVGLDTLYYGEFDNYGPGANTSLRVQWGGYSIMNVSEAVN 550

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME47_ARATH9.2e-12948.81Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana GN=P... [more]
PME25_ARATH7.1e-9752.17Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana GN=P... [more]
PME41_ARATH5.0e-5837.85Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana GN=P... [more]
PME20_ARATH4.3e-5436.70Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana GN=P... [more]
PME7_ARATH2.4e-5236.30Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana GN=PM... [more]
Match NameE-valueIdentityDescription
A0A0A0KUN8_CUCSA1.1e-23274.96Pectinesterase OS=Cucumis sativus GN=Csa_5G612810 PE=4 SV=1[more]
B9RPV5_RICCO4.1e-16055.40Pectinesterase OS=Ricinus communis GN=RCOM_1541960 PE=4 SV=1[more]
B9HVF5_POPTR2.8e-15352.75Pectinesterase OS=Populus trichocarpa GN=POPTR_0010s25400g PE=4 SV=2[more]
A0A067K1K9_JATCU4.9e-15353.91Pectinesterase OS=Jatropha curcas GN=JCGZ_18594 PE=4 SV=1[more]
B9HK85_POPTR1.6e-14851.66Pectinesterase OS=Populus trichocarpa GN=POPTR_0008s01180g PE=4 SV=2[more]
Match NameE-valueIdentityDescription
AT5G04970.15.2e-13048.81 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G10720.24.0e-9852.17 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G02330.12.8e-5937.85 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT2G47550.12.4e-5536.70 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT1G02810.11.3e-5336.30 Plant invertase/pectin methylesterase inhibitor superfamily[more]
Match NameE-valueIdentityDescription
gi|659091753|ref|XP_008446714.1|2.1e-23475.84PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Cucumis melo][more]
gi|449435184|ref|XP_004135375.1|1.5e-23274.96PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Cucumis sativus][more]
gi|255549442|ref|XP_002515774.1|5.9e-16055.40PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Ricinus communis... [more]
gi|566192712|ref|XP_002315399.2|4.1e-15352.75hypothetical protein POPTR_0010s25400g [Populus trichocarpa][more]
gi|802667492|ref|XP_012081346.1|7.0e-15353.91PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Jatropha curcas][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000070Pectinesterase_cat
IPR006501Pectinesterase_inhib_dom
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
GO:0004857enzyme inhibitor activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005618 cell wall
cellular_component GO:0005667 transcription factor complex
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 transcription factor activity, sequence-specific DNA binding
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0016787 hydrolase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG05G022100.1ClCG05G022100.1mRNA


Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 250..358
score: 2.5E-35coord: 359..477
score: 3.3
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 28..179
score: 3.2
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 28..175
score: 3.3
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 21..176
score: 1.2
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 3..152
score: 8.1
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 28..179
score: 1.83
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 245..488
score: 7.59
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 359..486
score: 3.8E-37coord: 247..358
score: 2.6
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 1..210
score: 1.9E-259coord: 231..493
score: 1.9E
NoneNo IPR availablePANTHERPTHR31707:SF21PECTINESTERASE/PECTINESTERASE INHIBITOR 25-RELATEDcoord: 1..210
score: 1.9E-259coord: 231..493
score: 1.9E