CmaCh02G002470 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh02G002470 vs. Swiss-Prot
Match: SBT16_ARATH (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 1138.6 bits (2944), Expect = 0.0e+00
Identity = 558/767 (72.75%), Postives = 657/767 (85.66%), Query Frame = 1

Query: 10  SSSIFFSLLLLIHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTSEFAESHKILHVY 69
           SS+I   L L     +  A    KTFIFR+D  + PS+FPTH+HWY++EFAE  +I+HVY
Sbjct: 3   SSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVY 62

Query: 70  DTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYG 129
            TVFHGFSA  T ++AD++  HP+VLAVFEDRRR+LHTTRSPQFLGL+NQ+GLWS+SDYG
Sbjct: 63  HTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYG 122

Query: 130 SDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGH 189
           SDVIIGVFDTGI PERRSFSD+NLGPIP+RW+GVC++G +F+ +NCNRKI+GARFF+KG 
Sbjct: 123 SDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQ 182

Query: 190 EAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPK 249
           +A       I GIN TVE+ SPRDADGHGTHT+STAAGRHAF+AS+ GYASG+AKGVAPK
Sbjct: 183 QAAV-----IGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPK 242

Query: 250 ARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDPIAIGAYG 309
           AR+A YKVCWK+SGC DSDILAAFD+AV DGVDVISISIGGGDG+++PYYLDPIAIG+YG
Sbjct: 243 ARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYG 302

Query: 310 AASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYA 369
           AAS+GIFVSSSAGN+GPNGMSVTNLAPW+TTVGA TIDRNFPA  I+G+  ++ GVSLYA
Sbjct: 303 AASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYA 362

Query: 370 GAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVAKGLVVKKAG 429
           G PLNG M+P+VYPGKSG  SASLCME++LDPK V GKIVICDRGSSPRVAKGLVVKKAG
Sbjct: 363 GVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAG 422

Query: 430 GVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTATIAFRGTRIGIK 489
           GVGMILANG SNGEGLVGDAH++PACAVGS EGD +KAYASS  NP A+I FRGT +GIK
Sbjct: 423 GVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIK 482

Query: 490 PAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKIEFNILSGT 549
           PAPV+ASFS RGPNGL+PEILKPD+IAPGVNILAAWTD VGPTGL  D RK EFNILSGT
Sbjct: 483 PAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGT 542

Query: 550 SMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEESTGKASTPYDFGA 609
           SMACPH+SGAAALLKSAH DWSPA IRSAMMTT  + DN  +   +ESTGK++TPYD+G+
Sbjct: 543 SMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGS 602

Query: 610 GHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKCP-ARKPLPGNLNYP 669
           GH+NLG AM+PGLVYDITN DY+ FLCSIGYG K IQVI+RTPV+CP  RKP PGNLNYP
Sbjct: 603 GHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYP 662

Query: 670 SIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVKPSKLVFSTAVKKQ 729
           SI AVF +  + L +K+ IRTATNVG A +VY+A+IE+P+GVTV VKP +LVF++AVK++
Sbjct: 663 SITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRR 722

Query: 730 SFVVAISANNQNLTLGNVGAVFGSLSWSD-GKHVVRMPLVVTKLEPL 775
           S+ V ++ N +N+ LG  GAVFGS++W D GKHVVR P+VVT+++ L
Sbjct: 723 SYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

BLAST of CmaCh02G002470 vs. Swiss-Prot
Match: SBT15_ARATH (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 4.6e-244
Identity = 447/779 (57.38%), Postives = 548/779 (70.35%), Query Frame = 1

Query: 14  FFSLLLLIHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTSEFAE----SHKILHVY 73
           FF LL L   S+  +     T+I  VD  AKPS+FPTHFHWYTS  A        I+H Y
Sbjct: 7   FFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTY 66

Query: 74  DTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRN--QRGLWSDSD 133
           DTVFHGFSA  T + A  +  HP V++V  ++ R LHTTRSP+FLGLR+  + GL  +SD
Sbjct: 67  DTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESD 126

Query: 134 YGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSK 193
           +GSD++IGV DTG+ PER SF D  LGP+P +WKG C     F    CNRK+VGARFF  
Sbjct: 127 FGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCG 186

Query: 194 GHEAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVA 253
           G+EA +        +N+T E+RSPRD+DGHGTHTAS +AGR+ F AS  GYA G+A G+A
Sbjct: 187 GYEATNGK------MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 246

Query: 254 PKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDPIAIGA 313
           PKARLA YKVCW NSGC+DSDILAAFD+AV DGVDVIS+S+GG   V  PYYLD IAIGA
Sbjct: 247 PKARLAAYKVCW-NSGCYDSDILAAFDTAVADGVDVISLSVGG---VVVPYYLDAIAIGA 306

Query: 314 YGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSL 373
           +GA  RGIFVS+SAGN GP  ++VTN+APW+TTVGAGTIDR+FPA V +GN + I GVS+
Sbjct: 307 FGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSV 366

Query: 374 YAGAPLN-GTMYPLVYPGK--SGE-LSASLCMESSLDPKIVTGKIVICDRGSSPRVAKGL 433
           Y G  L+ G MYPLVY G    G+  S+SLC+E SLDP +V GKIV+CDRG + R  KG 
Sbjct: 367 YGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGE 426

Query: 434 VVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYAS------SSRNPTA 493
           +V+K GG+GMI+ANG+ +GEGLV D H+LPA +VG+  GD ++ Y S      SS++PTA
Sbjct: 427 IVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTA 486

Query: 494 TIAFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFD 553
           TI F+GTR+GI+PAPVVASFSARGPN   PEILKPD+IAPG+NILAAW D +GP+G+  D
Sbjct: 487 TIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSD 546

Query: 554 TRKIEFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEES 613
            R+ EFNILSGTSMACPH+SG AALLK+AH DWSPAAIRSA++TTA   DN  +P  +ES
Sbjct: 547 NRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDES 606

Query: 614 TGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKCPA 673
           TG  S+  D+G+GHV+   AMDPGLVYDIT+ DY+NFLC+  Y    I  I+R    C  
Sbjct: 607 TGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDG 666

Query: 674 --RKPLPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVK 733
             R    GNLNYPS   VF    +   +  FIRT TNVG ++SVY+ KI  P+G TV V+
Sbjct: 667 ARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVE 726

Query: 734 PSKLVFSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPLVVTKLEPL 775
           P KL F    +K SFVV +      L+ G      G + WSDGK  V  PLVVT  +PL
Sbjct: 727 PEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQPL 775

BLAST of CmaCh02G002470 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 7.7e-207
Identity = 391/770 (50.78%), Postives = 522/770 (67.79%), Query Frame = 1

Query: 9   LSSSIFFSLLLL--IHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTSEF---AESH 68
           LSS+ FF LL L   H S+  +D    T+I  + +   PS F  H +WY S     ++S 
Sbjct: 6   LSSTAFFLLLCLGFCHVSSSSSDQG--TYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA 65

Query: 69  KILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGL-RNQRGL 128
           ++L+ Y+   HGFS   TQE+ADS+   P V++V  + R +LHTTR+P FLGL  +   L
Sbjct: 66  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 125

Query: 129 WSDSDYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGA 188
           + ++   SDV++GV DTG+ PE +S+SD   GPIP  WKG C+ GT FTA  CNRK++GA
Sbjct: 126 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 185

Query: 189 RFFSKGHEAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGI 248
           RFF++G+E+     GPI   +++ E RSPRD DGHGTHT+STAAG     ASL GYASG 
Sbjct: 186 RFFARGYESTM---GPI---DESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGT 245

Query: 249 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDP 308
           A+G+AP+AR+AVYKVCW   GCF SDILAA D A+ D V+V+S+S+GGG    + YY D 
Sbjct: 246 ARGMAPRARVAVYKVCWLG-GCFSSDILAAIDKAIADNVNVLSMSLGGG---MSDYYRDG 305

Query: 309 IAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKI 368
           +AIGA+ A  RGI VS SAGN GP+  S++N+APWITTVGAGT+DR+FPA+ I+GN +  
Sbjct: 306 VAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNF 365

Query: 369 HGVSLYAGAPLNGTMYPLVYPGK-SGELSASLCMESSLDPKIVTGKIVICDRGSSPRVAK 428
            GVSL+ G  L   + P +Y G  S   + +LCM  +L P+ V GKIV+CDRG + RV K
Sbjct: 366 TGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQK 425

Query: 429 GLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTATIAF 488
           G VVK AGGVGMILAN  +NGE LV DAH+LPA  VG   GD ++ Y ++  NPTA+I+ 
Sbjct: 426 GDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISI 485

Query: 489 RGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKI 548
            GT +G+KP+PVVA+FS+RGPN + P ILKPD+IAPGVNILAAWT   GPTGL  D+R++
Sbjct: 486 LGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRV 545

Query: 549 EFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEESTGKA 608
           EFNI+SGTSM+CPH+SG AALLKS H +WSPAAIRSA+MTTA  T    +P  + +TGK 
Sbjct: 546 EFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKP 605

Query: 609 STPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKC-PARKP 668
           STP+D GAGHV+   A +PGL+YD+T  DY+ FLC++ Y +  I+ +SR    C P++  
Sbjct: 606 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY 665

Query: 669 LPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEA-PKGVTVKVKPSKL 728
              +LNYPS    F+     +    + RT T+VG A + Y  K+ +   GV + V+P+ L
Sbjct: 666 SVADLNYPS----FAVNVDGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVL 725

Query: 729 VFSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPLVVT 770
            F  A +K+S+ V  + ++   +  N    FGS+ WSDGKHVV  P+ ++
Sbjct: 726 NFKEANEKKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAIS 755

BLAST of CmaCh02G002470 vs. Swiss-Prot
Match: SBT18_ARATH (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 4.7e-204
Identity = 377/768 (49.09%), Postives = 507/768 (66.02%), Query Frame = 1

Query: 1   MAACFATSLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTSEFA 60
           MA+  ++S S +I  + L L+  +T       KT+I RV+   KP  F TH  WYTS+  
Sbjct: 1   MASSSSSSSSITIITTFLFLLLHTTAK-----KTYIIRVNHSDKPESFLTHHDWYTSQLN 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADSV-GKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQ 120
               +L+ Y T FHGFSA     +ADS+     S+L +FED    LHTTR+P+FLGL ++
Sbjct: 61  SESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSE 120

Query: 121 RGLWSDSDYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKI 180
            G+       + VIIGV DTG+ PE RSF D ++  IP +WKG C++G+ F +K CN+K+
Sbjct: 121 FGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKL 180

Query: 181 VGARFFSKGHEAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYA 240
           +GAR FSKG +  S       G +   E  SPRD DGHGTHT++TAAG     AS  GYA
Sbjct: 181 IGARSFSKGFQMASGG-----GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYA 240

Query: 241 SGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYY 300
           +G A+G+A +AR+A YKVCW ++GCF SDILAA D A+ DGVDV+S+S+GGG   S PYY
Sbjct: 241 AGTARGMATRARVATYKVCW-STGCFGSDILAAMDRAILDGVDVLSLSLGGG---SAPYY 300

Query: 301 LDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNR 360
            D IAIGA+ A  RG+FVS SAGN GP   SV N+APW+ TVGAGT+DR+FPA   +GN 
Sbjct: 301 RDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNG 360

Query: 361 RKIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 420
           +++ GVSLY+G  +      LVY  K    S++LC+  SLD  IV GKIV+CDRG + RV
Sbjct: 361 KRLTGVSLYSGVGMGTKPLELVY-NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARV 420

Query: 421 AKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTATI 480
            KG VV+ AGG+GMI+AN  ++GE LV D+H+LPA AVG   GD ++ Y  S   PTA +
Sbjct: 421 EKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALL 480

Query: 481 AFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTR 540
            F+GT + +KP+PVVA+FS+RGPN + PEILKPD+I PGVNILA W+D +GPTGLD D+R
Sbjct: 481 VFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSR 540

Query: 541 KIEFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEESTG 600
           + +FNI+SGTSM+CPHISG A LLK+AH +WSP+AI+SA+MTTA + DN   P  + +  
Sbjct: 541 RTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADN 600

Query: 601 KASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGA-KMIQVISRTPVKCPAR 660
             S PY  G+GHV+   A+ PGLVYDI+  +Y+ FLCS+ Y    ++ ++ R  V C  +
Sbjct: 601 SLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKK 660

Query: 661 KPLPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVKPSK 720
              PG LNYPS   +F     V     + R  TNVGAA+SVY+  +     V + VKPSK
Sbjct: 661 FSDPGQLNYPSFSVLFGGKRVV----RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSK 720

Query: 721 LVFSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPL 767
           L F +  +K+ + V    + + +++ N  A FGS++WS+ +H VR P+
Sbjct: 721 LSFKSVGEKKRYTVTF-VSKKGVSMTN-KAEFGSITWSNPQHEVRSPV 747

BLAST of CmaCh02G002470 vs. Swiss-Prot
Match: SBT13_ARATH (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 8.5e-198
Identity = 376/779 (48.27%), Postives = 500/779 (64.18%), Query Frame = 1

Query: 18  LLLIHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTS------------EFAESHKI 77
           L+ +   T       KT++  +D+ A P  +  H  WY+S            E   +++I
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRI 79

Query: 78  LHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG--LW 137
           L+ Y T FHG +A  TQE+A+ + +   V+AV  + R +LHTTRSP FLGL  Q    +W
Sbjct: 80  LYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVW 139

Query: 138 SDSDYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGAR 197
           ++     DV++GV DTGI PE  SF+D  + P+P  W+G C+TG +F  +NCNRKIVGAR
Sbjct: 140 AERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGAR 199

Query: 198 FFSKGHEAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIA 257
            F +G+EA +        I++ +EY+SPRD DGHGTHTA+T AG     A+L G+A G A
Sbjct: 200 VFYRGYEAATGK------IDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTA 259

Query: 258 KGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDPI 317
           +G+A KAR+A YKVCW   GCF SDIL+A D AV DGV V+SIS+GGG  VST Y  D +
Sbjct: 260 RGMAQKARVAAYKVCWVG-GCFSSDILSAVDQAVADGVQVLSISLGGG--VST-YSRDSL 319

Query: 318 AIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIH 377
           +I  +GA   G+FVS SAGN GP+ +S+TN++PWITTVGA T+DR+FPA V +G  R   
Sbjct: 320 SIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFK 379

Query: 378 GVSLYAGAPL--NGTMYPLVYPGKSGELS--ASLCMESSLDPKIVTGKIVICDRGSSPRV 437
           GVSLY G  +      YPLVY G++       S C++ +LD + V GKIVICDRG +PRV
Sbjct: 380 GVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRV 439

Query: 438 AKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTATI 497
            KG VVK+AGG+GM+L N  +NGE LV D+H+LPA AVG  EG  +K YA +S+  TA++
Sbjct: 440 QKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASL 499

Query: 498 AFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTR 557
              GTRIGIKP+PVVA+FS+RGPN L+ EILKPD++APGVNILAAWT  + P+ L  D R
Sbjct: 500 EILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPR 559

Query: 558 KIEFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEESTG 617
           +++FNILSGTSM+CPH+SG AAL+KS H DWSPAAI+SA+MTTA + DN ++P T+ S  
Sbjct: 560 RVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGA 619

Query: 618 KASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVI----SRTPVKC 677
             S+PYD GAGH++   A DPGLVYDI   +Y  FLC+       ++V     +RT    
Sbjct: 620 APSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHT 679

Query: 678 PARKPLPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVK 737
            A+   PGNLNYP+I A+F   + V    +  RT TNVG   S Y+  +   KG +V V+
Sbjct: 680 LAKN--PGNLNYPAISALFPENTHV-KAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQ 739

Query: 738 PSKLVFSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPLVVTKLEPL 775
           P  L F++  +K S+ V      +          FG L W    H VR P+++T L PL
Sbjct: 740 PKTLNFTSKHQKLSYTVTFRTRFRMKR-----PEFGGLVWKSTTHKVRSPVIITWLPPL 780

BLAST of CmaCh02G002470 vs. TrEMBL
Match: A0A0A0LJ58_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G270180 PE=4 SV=1)

HSP 1 Score: 1377.8 bits (3565), Expect = 0.0e+00
Identity = 690/774 (89.15%), Postives = 730/774 (94.32%), Query Frame = 1

Query: 1   MAACFATSLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTSEFA 60
           MAA  +TSLSS  FFS LLL+  STV +  PLKTFI R+DRF+KPSVFPTH+HWYTSEF 
Sbjct: 1   MAAFLSTSLSS-FFFSFLLLL--STVSSHSPLKTFIVRIDRFSKPSVFPTHYHWYTSEFT 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           +S +ILHVYDTVFHGFSAT TQ+Q DS+GKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  QSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGI PERRSFSDVNLGPIPRRWKGVC+TGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAG+NA GPIIGINDT+EYRSPRDADGHGTHTASTAAGRH+FQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFD+AVNDGVDVISISIGGGDGVS+PYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIG+YGAAS+G+FVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFP+VV +GN R
Sbjct: 301 DPIAIGSYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGNGR 360

Query: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420
           KI+GVSLYAGAPLNGTMYPLVYPGKSG LS SLCME+SLDPK+VTGKIVICDRGSSPRVA
Sbjct: 361 KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAH+LPACAVGS EGDAMKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           F+GT IGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTD VGPTGLDFD RK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDKRK 540

Query: 541 IEFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEESTGK 600
            EFNILSGTSMACPH+SGAAALLKSAH DWSPAA+RSAMMTTA ITDNR QP TEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKCPARKP 660
            STPYDFGAGHVNLGLAMDPGL+YDITNTDY+NFLCSIGYG KMIQVI+RTPV+CP +KP
Sbjct: 601 PSTPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKP 660

Query: 661 LPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVKPSKLV 720
           LP NLNYPSIV VFSSLSK  STKSFIRTATNVG +NSVY+ KIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVTVFSSLSKGWSTKSFIRTATNVGPSNSVYRVKIEAPKGVTVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPLVVTKLEPL 775
           FST VKKQSFVVAISA+NQNL LG+VGAVFG LSWSDGKHVVR PLVVT+LEPL
Sbjct: 721 FSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of CmaCh02G002470 vs. TrEMBL
Match: W9QT04_9ROSA (Subtilisin-like protease OS=Morus notabilis GN=L484_024992 PE=4 SV=1)

HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 590/743 (79.41%), Postives = 668/743 (89.91%), Query Frame = 1

Query: 32  LKTFIFRVDRFAKPSVFPTHFHWYTSEFAESHKILHVYDTVFHGFSATFTQEQADSVGKH 91
           LKTFIFRVD   KPS+FPTH+HWYT+EFA+  +ILHVYDTVF+GFSA  + +Q     +H
Sbjct: 36  LKTFIFRVDSHTKPSIFPTHYHWYTTEFADPPQILHVYDTVFNGFSAVLSSDQVAYASRH 95

Query: 92  PSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGICPERRSFSDV 151
           PSVLAVFED+RRQLHTTRSPQFLGLRNQRGLWS+SDYGSDVIIGVFDTGI PERRSFSD+
Sbjct: 96  PSVLAVFEDKRRQLHTTRSPQFLGLRNQRGLWSESDYGSDVIIGVFDTGIWPERRSFSDL 155

Query: 152 NLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGHEAGSNADGPIIGINDTVEYRSP 211
           NLGPIP RWKGVC++G KF+ +NCNRK++GARFFSKGHEA  +  GPI G+NDT+E+RSP
Sbjct: 156 NLGPIPSRWKGVCESGAKFSVRNCNRKLIGARFFSKGHEAAGSIGGPISGVNDTLEFRSP 215

Query: 212 RDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILA 271
           RDADGHGTHTASTAAGR+AF+AS+ GYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILA
Sbjct: 216 RDADGHGTHTASTAAGRYAFEASMAGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILA 275

Query: 272 AFDSAVNDGVDVISISIGGGDGVSTPYYLDPIAIGAYGAASRGIFVSSSAGNDGPNGMSV 331
           AFD+AVNDGVDVISISIGGGDG+S+PYYLDPIAIGAYGA S+G+FVSSSAGNDGPNGMSV
Sbjct: 276 AFDAAVNDGVDVISISIGGGDGISSPYYLDPIAIGAYGAVSKGVFVSSSAGNDGPNGMSV 335

Query: 332 TNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYAGAPLNGTMYPLVYPGKSGELSA 391
           TNLAPW+TTVGAGTIDR FPAV+++G+ R++ GVSLYAGAPL G MYPLVYPGKSG L A
Sbjct: 336 TNLAPWMTTVGAGTIDRTFPAVIVLGDGRRLSGVSLYAGAPLKGKMYPLVYPGKSGMLPA 395

Query: 392 SLCMESSLDPKIVTGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHI 451
           SLCME+SLDP +V GKIVICDRGSSPRVAKG+VVKKAGGVGMIL+NGIS G GLVGDAHI
Sbjct: 396 SLCMENSLDPNLVRGKIVICDRGSSPRVAKGMVVKKAGGVGMILSNGISQGGGLVGDAHI 455

Query: 452 LPACAVGSVEGDAMKAYASSSRNPTATIAFRGTRIGIKPAPVVASFSARGPNGLNPEILK 511
           LPACAVGS EG+A+KAY SS+ NPTATI F+GT IGIKPAP+VASFS RGPN +NPEILK
Sbjct: 456 LPACAVGSDEGNAVKAYVSSASNPTATIDFQGTVIGIKPAPIVASFSGRGPNSVNPEILK 515

Query: 512 PDMIAPGVNILAAWTDVVGPTGLDFDTRKIEFNILSGTSMACPHISGAAALLKSAHLDWS 571
           PD+IAPGVNILAAWTD VGPTGLD D RK EFNILSGTSMACPH+SGAAALLKSAH DWS
Sbjct: 516 PDLIAPGVNILAAWTDAVGPTGLDSDKRKTEFNILSGTSMACPHVSGAAALLKSAHPDWS 575

Query: 572 PAAIRSAMMTTAKITDNRWQPTTEESTGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDY 631
           PAAIRSAMMTTA I DNR Q  T+ESTGK+STPYD GAGH+NL  AMDPGLVYDITN D+
Sbjct: 576 PAAIRSAMMTTASIVDNRNQLMTDESTGKSSTPYDLGAGHLNLDRAMDPGLVYDITNDDH 635

Query: 632 VNFLCSIGYGAKMIQVISRTPVKCPARKPLPGNLNYPSIVAVFSSLSKVLSTKSFIRTAT 691
           VNFLCSIGYG K+IQVI+RTPVKCP ++PLP NLNYPS+ A+F + S+  ++K FIRT T
Sbjct: 636 VNFLCSIGYGPKVIQVITRTPVKCPVKRPLPENLNYPSMAALFPTSSRGSTSKMFIRTVT 695

Query: 692 NVGAANSVYQAKIEAPKGVTVKVKPSKLVFSTAVKKQSFVVAISANNQNLTLGNVGAVFG 751
           NVGA NSVY+A+IEAPKGVTV+VKP+KLVF+ AVKKQSFVV ++A+ ++L LG  GA FG
Sbjct: 696 NVGAPNSVYRARIEAPKGVTVRVKPAKLVFTEAVKKQSFVVTVTADARSLVLGESGANFG 755

Query: 752 SLSWSDGKHVVRMPLVVTKLEPL 775
           SLSW+DGKHVVR P+VVT+++PL
Sbjct: 756 SLSWTDGKHVVRSPIVVTEIQPL 778

BLAST of CmaCh02G002470 vs. TrEMBL
Match: A0A061DZB4_THECC (Subtilisin-like serine protease 2 OS=Theobroma cacao GN=TCM_006646 PE=4 SV=1)

HSP 1 Score: 1208.4 bits (3125), Expect = 0.0e+00
Identity = 599/769 (77.89%), Postives = 671/769 (87.26%), Query Frame = 1

Query: 11  SSIFFSLLLLIHGSTVPAD----PPLKTFIFRVDRFAKPSVFPTHFHWYTSEFAESHKIL 70
           SS    L LL   S  P        +KTFIFRVD  +KPS+FPTH+HWYTSEFAE  +IL
Sbjct: 6   SSFLPLLFLLFISSDFPKSFSSYQTVKTFIFRVDSESKPSIFPTHYHWYTSEFAEPTRIL 65

Query: 71  HVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDS 130
           HVYDTVFHGFSA  T+  A S+  HPSVLAVFEDRRR+LHTTRSPQFLGLRNQ GLWSDS
Sbjct: 66  HVYDTVFHGFSAVVTETHAASLSNHPSVLAVFEDRRRELHTTRSPQFLGLRNQHGLWSDS 125

Query: 131 DYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFS 190
           DYGSDVIIGVFDTGI PERRSFSD NLGPIP RWKGVCQTG KF AKNCNRK++GARFFS
Sbjct: 126 DYGSDVIIGVFDTGIWPERRSFSDTNLGPIPARWKGVCQTGAKFVAKNCNRKLIGARFFS 185

Query: 191 KGHEAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGV 250
           KGHEA +   GPI GIN+T+E+ SPRDADGHGTHTASTAAGRH+F+AS+EGYA+GIAKGV
Sbjct: 186 KGHEAAAGLGGPIAGINETIEFMSPRDADGHGTHTASTAAGRHSFRASMEGYAAGIAKGV 245

Query: 251 APKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDPIAIG 310
           APKARLAVYKVCWKNSGCFDSDILAAFD AVNDGVDVISISIGGGDG+S+PYYLDPIAIG
Sbjct: 246 APKARLAVYKVCWKNSGCFDSDILAAFDGAVNDGVDVISISIGGGDGISSPYYLDPIAIG 305

Query: 311 AYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVS 370
           AYGA SRG+FVSSSAGNDGPN MSVTNLAPW+ TVGAGTIDRNFPA VI+G+ R+++GVS
Sbjct: 306 AYGAVSRGVFVSSSAGNDGPNLMSVTNLAPWLVTVGAGTIDRNFPADVILGDARRLNGVS 365

Query: 371 LYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVAKGLVVK 430
           LY+G  L G MYPLVYPGKSG LSASLCME+SLDP +V GKIVICDRGSSPRVAKGLVV+
Sbjct: 366 LYSGEQLKGKMYPLVYPGKSGVLSASLCMENSLDPSVVKGKIVICDRGSSPRVAKGLVVQ 425

Query: 431 KAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTATIAFRGTRI 490
           KAGGVGMILANG+SNGEGLVGDAHILPACA+GS EGDA+K+Y SSS NPTATI F+GT I
Sbjct: 426 KAGGVGMILANGVSNGEGLVGDAHILPACALGSDEGDAVKSYVSSSANPTATIDFKGTVI 485

Query: 491 GIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKIEFNIL 550
           GIKPAPVVASF+ RGPNGLNPEILKPD+IAPGVNILAAWTD VGPTGLD D RK EFNIL
Sbjct: 486 GIKPAPVVASFTGRGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDSDQRKTEFNIL 545

Query: 551 SGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEESTGKASTPYD 610
           SGTSMACPH+SGAAALLKSAH DWSPAAIRSAMMTTA ITDN+ QP  +E+TGK STPYD
Sbjct: 546 SGTSMACPHVSGAAALLKSAHPDWSPAAIRSAMMTTASITDNKNQPMIDEATGKQSTPYD 605

Query: 611 FGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKCPARKPLPGNLN 670
           FGAGH+NL  AMDPGL+YDITN DY NFLC+IGY  K++QV++R+P  CP +KPLP NLN
Sbjct: 606 FGAGHLNLDRAMDPGLIYDITNNDYENFLCAIGYNPKLVQVVTRSPAVCPMKKPLPENLN 665

Query: 671 YPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVKPSKLVFSTAVK 730
           YPSI A+FS+ S+  ++K+FIRT TNVG AN+VY AKIEAPKGV V VKP +LVF+ AVK
Sbjct: 666 YPSIAALFSTTSRGPTSKTFIRTVTNVGQANAVYVAKIEAPKGVRVTVKPVELVFTPAVK 725

Query: 731 KQSFVVAISANNQNLTLGNVGAVFGSLSWSDG-KHVVRMPLVVTKLEPL 775
           K+SF V I+A++++L + + GAVFGSLSW+DG KHVVR P+VVT+L+PL
Sbjct: 726 KRSFFVTITADSKHLVVDDSGAVFGSLSWTDGNKHVVRSPIVVTQLDPL 774

BLAST of CmaCh02G002470 vs. TrEMBL
Match: A0A067FX47_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g004010mg PE=4 SV=1)

HSP 1 Score: 1206.4 bits (3120), Expect = 0.0e+00
Identity = 601/779 (77.15%), Postives = 680/779 (87.29%), Query Frame = 1

Query: 1   MAACFATSLSSSIFFSLLL---LIHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTS 60
           MAA   +SL     F LLL    +   T+  D  +KTFIFR+D  +KPS+FPTH+HWY+S
Sbjct: 1   MAAIPYSSLPLQFLFFLLLSGSFLQTRTLSTDQTVKTFIFRIDSQSKPSIFPTHYHWYSS 60

Query: 61  EFAESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLR 120
           EFA   +ILH YDTVFHGFSAT + +QA S+ +HPSVLAV ED+RRQLHTTRSPQFLGLR
Sbjct: 61  EFASPVQILHTYDTVFHGFSATLSPDQAASLSRHPSVLAVIEDQRRQLHTTRSPQFLGLR 120

Query: 121 NQRGLWSDSDYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNR 180
           NQ+GLWS+SDYGSDVIIGVFDTGI PERRSFSD+N+G IP +WKGVCQ G KFTAKNCN+
Sbjct: 121 NQQGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNIGSIPSKWKGVCQVGVKFTAKNCNK 180

Query: 181 KIVGARFFSKGHEAGSNADGPIIG-INDTVEYRSPRDADGHGTHTASTAAGRHAFQASLE 240
           KI+GARFFSKGHEA   + GPI G IN+TVE+ SPRDADGHGTHTASTAAGRHAF+AS+E
Sbjct: 181 KIIGARFFSKGHEAAGGSAGPIGGGINETVEFMSPRDADGHGTHTASTAAGRHAFRASME 240

Query: 241 GYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVST 300
           GYA+G+AKGVAPKARLAVYKVCWKN+GCFDSDILAAFD+AVNDGVDVISISIGGGDG+S+
Sbjct: 241 GYAAGVAKGVAPKARLAVYKVCWKNAGCFDSDILAAFDAAVNDGVDVISISIGGGDGISS 300

Query: 301 PYYLDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIM 360
           PYYLDPIAIG+YGAASRG+FVSSSAGNDGPNGMSVTNLAPWI TVGAGTIDRNFPA V +
Sbjct: 301 PYYLDPIAIGSYGAASRGVFVSSSAGNDGPNGMSVTNLAPWIVTVGAGTIDRNFPAEVRL 360

Query: 361 GNRRKIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSS 420
           G+ R++ GVSLYAGAPL+  MYPL+YPGKSG LSASLCME+SLDP +V GKIVICDRGSS
Sbjct: 361 GDGRRLSGVSLYAGAPLSEKMYPLIYPGKSGVLSASLCMENSLDPNLVRGKIVICDRGSS 420

Query: 421 PRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPT 480
           PRVAKGLVVKKAGGVGMILANGISNGEGLVGDAH+LPACA+GS EGDA+KAY SS+ NPT
Sbjct: 421 PRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACALGSDEGDAVKAYISSTANPT 480

Query: 481 ATIAFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDF 540
           ATI F+GT +GIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWT+ VGPTGLD 
Sbjct: 481 ATIDFKGTILGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTEAVGPTGLDS 540

Query: 541 DTRKIEFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEE 600
           D RK EFNILSGTSMACPH+SGAAALLKSAH DWSPAAIRSAMMTTA I DN  QP T+E
Sbjct: 541 DLRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAAIRSAMMTTASIVDNSNQPMTDE 600

Query: 601 STGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKCP 660
           +TG ASTPYDFGAGHVNL  AMDPGLVYDITN DYVNFLC+ GYG K+IQVI+R P +CP
Sbjct: 601 ATGNASTPYDFGAGHVNLDRAMDPGLVYDITNDDYVNFLCANGYGPKLIQVITRIPARCP 660

Query: 661 ARKPLPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAP-KGVTVKVK 720
           A++P P NLNYPSI A+FS+ S+ +S+KSFIRT TNVG  N+VY  K+ +P KGVTV VK
Sbjct: 661 AKRPRPENLNYPSIAALFSTQSRGVSSKSFIRTVTNVGQPNAVYTVKVVSPEKGVTVTVK 720

Query: 721 PSKLVFSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPLVVTKLEPL 775
           PS+LVF+  VKK SFVV ++A+++NL L + GA FGS+SWSDGKH VR PLVVT+L+PL
Sbjct: 721 PSRLVFTEGVKKSSFVVTVTADSKNLVLNDSGAAFGSISWSDGKHEVRSPLVVTQLDPL 779

BLAST of CmaCh02G002470 vs. TrEMBL
Match: V4SFS4_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027859mg PE=4 SV=1)

HSP 1 Score: 1206.4 bits (3120), Expect = 0.0e+00
Identity = 601/779 (77.15%), Postives = 680/779 (87.29%), Query Frame = 1

Query: 1   MAACFATSLSSSIFFSLLL---LIHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTS 60
           MAA   +SL     F LLL    +   T+  D  +KTFIFR+D  +KPS+FPTH+HWY+S
Sbjct: 1   MAAIPYSSLPLQFLFFLLLSGSFLQTRTLSTDQTVKTFIFRIDSQSKPSIFPTHYHWYSS 60

Query: 61  EFAESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLR 120
           EFA   +ILH YDTVFHGFSAT + +QA S+ +HPSVLAV ED+RRQLHTTRSPQFLGLR
Sbjct: 61  EFASPVQILHTYDTVFHGFSATLSPDQAASLSRHPSVLAVIEDQRRQLHTTRSPQFLGLR 120

Query: 121 NQRGLWSDSDYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNR 180
           NQ+GLWS+SDYGSDVIIGVFDTGI PERRSFSD+N+G IP +WKGVCQ G KFTAKNCN+
Sbjct: 121 NQQGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNIGSIPSKWKGVCQVGVKFTAKNCNK 180

Query: 181 KIVGARFFSKGHEAGSNADGPIIG-INDTVEYRSPRDADGHGTHTASTAAGRHAFQASLE 240
           KI+GARFFSKGHEA   + GPI G IN+TVE+ SPRDADGHGTHTASTAAGRHAF+AS+E
Sbjct: 181 KIIGARFFSKGHEAAGGSAGPIGGGINETVEFMSPRDADGHGTHTASTAAGRHAFRASME 240

Query: 241 GYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVST 300
           GYA+G+AKGVAPKARLAVYKVCWKN+GCFDSDILAAFD+AVNDGVDVISISIGGGDG+S+
Sbjct: 241 GYAAGVAKGVAPKARLAVYKVCWKNAGCFDSDILAAFDAAVNDGVDVISISIGGGDGISS 300

Query: 301 PYYLDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIM 360
           PYYLDPIAIG+YGAASRG+FVSSSAGNDGPNGMSVTNLAPWI TVGAGTIDRNFPA V +
Sbjct: 301 PYYLDPIAIGSYGAASRGVFVSSSAGNDGPNGMSVTNLAPWIVTVGAGTIDRNFPAEVRL 360

Query: 361 GNRRKIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSS 420
           G+ R++ GVSLYAGAPL+  MYPL+YPGKSG LSASLCME+SLDP +V GKIVICDRGSS
Sbjct: 361 GDGRRLSGVSLYAGAPLSEKMYPLIYPGKSGVLSASLCMENSLDPNLVRGKIVICDRGSS 420

Query: 421 PRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPT 480
           PRVAKGLVVKKAGGVGMILANGISNGEGLVGDAH+LPACA+GS EGDA+KAY SS+ NPT
Sbjct: 421 PRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACALGSDEGDAVKAYISSTANPT 480

Query: 481 ATIAFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDF 540
           ATI F+GT +GIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWT+ VGPTGLD 
Sbjct: 481 ATIDFKGTILGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTEAVGPTGLDS 540

Query: 541 DTRKIEFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEE 600
           D RK EFNILSGTSMACPH+SGAAALLKSAH DWSPAAIRSAMMTTA I DN  QP T+E
Sbjct: 541 DLRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAAIRSAMMTTASIVDNSNQPMTDE 600

Query: 601 STGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKCP 660
           +TG ASTPYDFGAGHVNL  AMDPGLVYDITN DYVNFLC+ GYG K+IQVI+R P +CP
Sbjct: 601 ATGNASTPYDFGAGHVNLDRAMDPGLVYDITNDDYVNFLCANGYGPKLIQVITRIPARCP 660

Query: 661 ARKPLPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAP-KGVTVKVK 720
           A++P P NLNYPSI A+FS+ S+ +S+KSFIRT TNVG  N+VY  K+ +P KGVTV VK
Sbjct: 661 AKRPRPENLNYPSIAALFSTQSRGVSSKSFIRTVTNVGQPNAVYTVKVVSPEKGVTVTVK 720

Query: 721 PSKLVFSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPLVVTKLEPL 775
           PS+LVF+  VKK SFVV ++A+++NL L + GA FGS+SWSDGKH VR PLVVT+L+PL
Sbjct: 721 PSRLVFTEGVKKSSFVVTVTADSKNLVLNDSGAAFGSISWSDGKHEVRSPLVVTQLDPL 779

BLAST of CmaCh02G002470 vs. TAIR10
Match: AT4G34980.1 (AT4G34980.1 subtilisin-like serine protease 2)

HSP 1 Score: 1138.6 bits (2944), Expect = 0.0e+00
Identity = 558/767 (72.75%), Postives = 657/767 (85.66%), Query Frame = 1

Query: 10  SSSIFFSLLLLIHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTSEFAESHKILHVY 69
           SS+I   L L     +  A    KTFIFR+D  + PS+FPTH+HWY++EFAE  +I+HVY
Sbjct: 3   SSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVY 62

Query: 70  DTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYG 129
            TVFHGFSA  T ++AD++  HP+VLAVFEDRRR+LHTTRSPQFLGL+NQ+GLWS+SDYG
Sbjct: 63  HTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYG 122

Query: 130 SDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGH 189
           SDVIIGVFDTGI PERRSFSD+NLGPIP+RW+GVC++G +F+ +NCNRKI+GARFF+KG 
Sbjct: 123 SDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQ 182

Query: 190 EAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPK 249
           +A       I GIN TVE+ SPRDADGHGTHT+STAAGRHAF+AS+ GYASG+AKGVAPK
Sbjct: 183 QAAV-----IGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPK 242

Query: 250 ARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDPIAIGAYG 309
           AR+A YKVCWK+SGC DSDILAAFD+AV DGVDVISISIGGGDG+++PYYLDPIAIG+YG
Sbjct: 243 ARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYG 302

Query: 310 AASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYA 369
           AAS+GIFVSSSAGN+GPNGMSVTNLAPW+TTVGA TIDRNFPA  I+G+  ++ GVSLYA
Sbjct: 303 AASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYA 362

Query: 370 GAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVAKGLVVKKAG 429
           G PLNG M+P+VYPGKSG  SASLCME++LDPK V GKIVICDRGSSPRVAKGLVVKKAG
Sbjct: 363 GVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAG 422

Query: 430 GVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTATIAFRGTRIGIK 489
           GVGMILANG SNGEGLVGDAH++PACAVGS EGD +KAYASS  NP A+I FRGT +GIK
Sbjct: 423 GVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIK 482

Query: 490 PAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKIEFNILSGT 549
           PAPV+ASFS RGPNGL+PEILKPD+IAPGVNILAAWTD VGPTGL  D RK EFNILSGT
Sbjct: 483 PAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGT 542

Query: 550 SMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEESTGKASTPYDFGA 609
           SMACPH+SGAAALLKSAH DWSPA IRSAMMTT  + DN  +   +ESTGK++TPYD+G+
Sbjct: 543 SMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGS 602

Query: 610 GHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKCP-ARKPLPGNLNYP 669
           GH+NLG AM+PGLVYDITN DY+ FLCSIGYG K IQVI+RTPV+CP  RKP PGNLNYP
Sbjct: 603 GHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYP 662

Query: 670 SIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVKPSKLVFSTAVKKQ 729
           SI AVF +  + L +K+ IRTATNVG A +VY+A+IE+P+GVTV VKP +LVF++AVK++
Sbjct: 663 SITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRR 722

Query: 730 SFVVAISANNQNLTLGNVGAVFGSLSWSD-GKHVVRMPLVVTKLEPL 775
           S+ V ++ N +N+ LG  GAVFGS++W D GKHVVR P+VVT+++ L
Sbjct: 723 SYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

BLAST of CmaCh02G002470 vs. TAIR10
Match: AT3G14240.1 (AT3G14240.1 Subtilase family protein)

HSP 1 Score: 845.5 bits (2183), Expect = 2.6e-245
Identity = 447/779 (57.38%), Postives = 548/779 (70.35%), Query Frame = 1

Query: 14  FFSLLLLIHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTSEFAE----SHKILHVY 73
           FF LL L   S+  +     T+I  VD  AKPS+FPTHFHWYTS  A        I+H Y
Sbjct: 7   FFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTY 66

Query: 74  DTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRN--QRGLWSDSD 133
           DTVFHGFSA  T + A  +  HP V++V  ++ R LHTTRSP+FLGLR+  + GL  +SD
Sbjct: 67  DTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESD 126

Query: 134 YGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSK 193
           +GSD++IGV DTG+ PER SF D  LGP+P +WKG C     F    CNRK+VGARFF  
Sbjct: 127 FGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCG 186

Query: 194 GHEAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVA 253
           G+EA +        +N+T E+RSPRD+DGHGTHTAS +AGR+ F AS  GYA G+A G+A
Sbjct: 187 GYEATNGK------MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 246

Query: 254 PKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDPIAIGA 313
           PKARLA YKVCW NSGC+DSDILAAFD+AV DGVDVIS+S+GG   V  PYYLD IAIGA
Sbjct: 247 PKARLAAYKVCW-NSGCYDSDILAAFDTAVADGVDVISLSVGG---VVVPYYLDAIAIGA 306

Query: 314 YGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSL 373
           +GA  RGIFVS+SAGN GP  ++VTN+APW+TTVGAGTIDR+FPA V +GN + I GVS+
Sbjct: 307 FGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSV 366

Query: 374 YAGAPLN-GTMYPLVYPGK--SGE-LSASLCMESSLDPKIVTGKIVICDRGSSPRVAKGL 433
           Y G  L+ G MYPLVY G    G+  S+SLC+E SLDP +V GKIV+CDRG + R  KG 
Sbjct: 367 YGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGE 426

Query: 434 VVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYAS------SSRNPTA 493
           +V+K GG+GMI+ANG+ +GEGLV D H+LPA +VG+  GD ++ Y S      SS++PTA
Sbjct: 427 IVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTA 486

Query: 494 TIAFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFD 553
           TI F+GTR+GI+PAPVVASFSARGPN   PEILKPD+IAPG+NILAAW D +GP+G+  D
Sbjct: 487 TIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSD 546

Query: 554 TRKIEFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEES 613
            R+ EFNILSGTSMACPH+SG AALLK+AH DWSPAAIRSA++TTA   DN  +P  +ES
Sbjct: 547 NRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDES 606

Query: 614 TGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKCPA 673
           TG  S+  D+G+GHV+   AMDPGLVYDIT+ DY+NFLC+  Y    I  I+R    C  
Sbjct: 607 TGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDG 666

Query: 674 --RKPLPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVK 733
             R    GNLNYPS   VF    +   +  FIRT TNVG ++SVY+ KI  P+G TV V+
Sbjct: 667 ARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVE 726

Query: 734 PSKLVFSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPLVVTKLEPL 775
           P KL F    +K SFVV +      L+ G      G + WSDGK  V  PLVVT  +PL
Sbjct: 727 PEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQPL 775

BLAST of CmaCh02G002470 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 721.8 bits (1862), Expect = 4.3e-208
Identity = 391/770 (50.78%), Postives = 522/770 (67.79%), Query Frame = 1

Query: 9   LSSSIFFSLLLL--IHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTSEF---AESH 68
           LSS+ FF LL L   H S+  +D    T+I  + +   PS F  H +WY S     ++S 
Sbjct: 6   LSSTAFFLLLCLGFCHVSSSSSDQG--TYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA 65

Query: 69  KILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGL-RNQRGL 128
           ++L+ Y+   HGFS   TQE+ADS+   P V++V  + R +LHTTR+P FLGL  +   L
Sbjct: 66  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 125

Query: 129 WSDSDYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGA 188
           + ++   SDV++GV DTG+ PE +S+SD   GPIP  WKG C+ GT FTA  CNRK++GA
Sbjct: 126 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 185

Query: 189 RFFSKGHEAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGI 248
           RFF++G+E+     GPI   +++ E RSPRD DGHGTHT+STAAG     ASL GYASG 
Sbjct: 186 RFFARGYESTM---GPI---DESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGT 245

Query: 249 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDP 308
           A+G+AP+AR+AVYKVCW   GCF SDILAA D A+ D V+V+S+S+GGG    + YY D 
Sbjct: 246 ARGMAPRARVAVYKVCWLG-GCFSSDILAAIDKAIADNVNVLSMSLGGG---MSDYYRDG 305

Query: 309 IAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKI 368
           +AIGA+ A  RGI VS SAGN GP+  S++N+APWITTVGAGT+DR+FPA+ I+GN +  
Sbjct: 306 VAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNF 365

Query: 369 HGVSLYAGAPLNGTMYPLVYPGK-SGELSASLCMESSLDPKIVTGKIVICDRGSSPRVAK 428
            GVSL+ G  L   + P +Y G  S   + +LCM  +L P+ V GKIV+CDRG + RV K
Sbjct: 366 TGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQK 425

Query: 429 GLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTATIAF 488
           G VVK AGGVGMILAN  +NGE LV DAH+LPA  VG   GD ++ Y ++  NPTA+I+ 
Sbjct: 426 GDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISI 485

Query: 489 RGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKI 548
            GT +G+KP+PVVA+FS+RGPN + P ILKPD+IAPGVNILAAWT   GPTGL  D+R++
Sbjct: 486 LGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRV 545

Query: 549 EFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEESTGKA 608
           EFNI+SGTSM+CPH+SG AALLKS H +WSPAAIRSA+MTTA  T    +P  + +TGK 
Sbjct: 546 EFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKP 605

Query: 609 STPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKC-PARKP 668
           STP+D GAGHV+   A +PGL+YD+T  DY+ FLC++ Y +  I+ +SR    C P++  
Sbjct: 606 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY 665

Query: 669 LPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEA-PKGVTVKVKPSKL 728
              +LNYPS    F+     +    + RT T+VG A + Y  K+ +   GV + V+P+ L
Sbjct: 666 SVADLNYPS----FAVNVDGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVL 725

Query: 729 VFSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPLVVT 770
            F  A +K+S+ V  + ++   +  N    FGS+ WSDGKHVV  P+ ++
Sbjct: 726 NFKEANEKKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAIS 755

BLAST of CmaCh02G002470 vs. TAIR10
Match: AT2G05920.1 (AT2G05920.1 Subtilase family protein)

HSP 1 Score: 712.6 bits (1838), Expect = 2.6e-205
Identity = 377/768 (49.09%), Postives = 507/768 (66.02%), Query Frame = 1

Query: 1   MAACFATSLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTSEFA 60
           MA+  ++S S +I  + L L+  +T       KT+I RV+   KP  F TH  WYTS+  
Sbjct: 1   MASSSSSSSSITIITTFLFLLLHTTAK-----KTYIIRVNHSDKPESFLTHHDWYTSQLN 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADSV-GKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQ 120
               +L+ Y T FHGFSA     +ADS+     S+L +FED    LHTTR+P+FLGL ++
Sbjct: 61  SESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSE 120

Query: 121 RGLWSDSDYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKI 180
            G+       + VIIGV DTG+ PE RSF D ++  IP +WKG C++G+ F +K CN+K+
Sbjct: 121 FGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKL 180

Query: 181 VGARFFSKGHEAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYA 240
           +GAR FSKG +  S       G +   E  SPRD DGHGTHT++TAAG     AS  GYA
Sbjct: 181 IGARSFSKGFQMASGG-----GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYA 240

Query: 241 SGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYY 300
           +G A+G+A +AR+A YKVCW ++GCF SDILAA D A+ DGVDV+S+S+GGG   S PYY
Sbjct: 241 AGTARGMATRARVATYKVCW-STGCFGSDILAAMDRAILDGVDVLSLSLGGG---SAPYY 300

Query: 301 LDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNR 360
            D IAIGA+ A  RG+FVS SAGN GP   SV N+APW+ TVGAGT+DR+FPA   +GN 
Sbjct: 301 RDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNG 360

Query: 361 RKIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 420
           +++ GVSLY+G  +      LVY  K    S++LC+  SLD  IV GKIV+CDRG + RV
Sbjct: 361 KRLTGVSLYSGVGMGTKPLELVY-NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARV 420

Query: 421 AKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTATI 480
            KG VV+ AGG+GMI+AN  ++GE LV D+H+LPA AVG   GD ++ Y  S   PTA +
Sbjct: 421 EKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALL 480

Query: 481 AFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTR 540
            F+GT + +KP+PVVA+FS+RGPN + PEILKPD+I PGVNILA W+D +GPTGLD D+R
Sbjct: 481 VFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSR 540

Query: 541 KIEFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEESTG 600
           + +FNI+SGTSM+CPHISG A LLK+AH +WSP+AI+SA+MTTA + DN   P  + +  
Sbjct: 541 RTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADN 600

Query: 601 KASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGA-KMIQVISRTPVKCPAR 660
             S PY  G+GHV+   A+ PGLVYDI+  +Y+ FLCS+ Y    ++ ++ R  V C  +
Sbjct: 601 SLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKK 660

Query: 661 KPLPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVKPSK 720
              PG LNYPS   +F     V     + R  TNVGAA+SVY+  +     V + VKPSK
Sbjct: 661 FSDPGQLNYPSFSVLFGGKRVV----RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSK 720

Query: 721 LVFSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPL 767
           L F +  +K+ + V    + + +++ N  A FGS++WS+ +H VR P+
Sbjct: 721 LSFKSVGEKKRYTVTF-VSKKGVSMTN-KAEFGSITWSNPQHEVRSPV 747

BLAST of CmaCh02G002470 vs. TAIR10
Match: AT5G51750.1 (AT5G51750.1 subtilase 1.3)

HSP 1 Score: 691.8 bits (1784), Expect = 4.8e-199
Identity = 376/779 (48.27%), Postives = 500/779 (64.18%), Query Frame = 1

Query: 18  LLLIHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTS------------EFAESHKI 77
           L+ +   T       KT++  +D+ A P  +  H  WY+S            E   +++I
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRI 79

Query: 78  LHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG--LW 137
           L+ Y T FHG +A  TQE+A+ + +   V+AV  + R +LHTTRSP FLGL  Q    +W
Sbjct: 80  LYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVW 139

Query: 138 SDSDYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGAR 197
           ++     DV++GV DTGI PE  SF+D  + P+P  W+G C+TG +F  +NCNRKIVGAR
Sbjct: 140 AERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGAR 199

Query: 198 FFSKGHEAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIA 257
            F +G+EA +        I++ +EY+SPRD DGHGTHTA+T AG     A+L G+A G A
Sbjct: 200 VFYRGYEAATGK------IDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTA 259

Query: 258 KGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDPI 317
           +G+A KAR+A YKVCW   GCF SDIL+A D AV DGV V+SIS+GGG  VST Y  D +
Sbjct: 260 RGMAQKARVAAYKVCWVG-GCFSSDILSAVDQAVADGVQVLSISLGGG--VST-YSRDSL 319

Query: 318 AIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIH 377
           +I  +GA   G+FVS SAGN GP+ +S+TN++PWITTVGA T+DR+FPA V +G  R   
Sbjct: 320 SIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFK 379

Query: 378 GVSLYAGAPL--NGTMYPLVYPGKSGELS--ASLCMESSLDPKIVTGKIVICDRGSSPRV 437
           GVSLY G  +      YPLVY G++       S C++ +LD + V GKIVICDRG +PRV
Sbjct: 380 GVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRV 439

Query: 438 AKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTATI 497
            KG VVK+AGG+GM+L N  +NGE LV D+H+LPA AVG  EG  +K YA +S+  TA++
Sbjct: 440 QKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASL 499

Query: 498 AFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTR 557
              GTRIGIKP+PVVA+FS+RGPN L+ EILKPD++APGVNILAAWT  + P+ L  D R
Sbjct: 500 EILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPR 559

Query: 558 KIEFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEESTG 617
           +++FNILSGTSM+CPH+SG AAL+KS H DWSPAAI+SA+MTTA + DN ++P T+ S  
Sbjct: 560 RVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGA 619

Query: 618 KASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVI----SRTPVKC 677
             S+PYD GAGH++   A DPGLVYDI   +Y  FLC+       ++V     +RT    
Sbjct: 620 APSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHT 679

Query: 678 PARKPLPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVK 737
            A+   PGNLNYP+I A+F   + V    +  RT TNVG   S Y+  +   KG +V V+
Sbjct: 680 LAKN--PGNLNYPAISALFPENTHV-KAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQ 739

Query: 738 PSKLVFSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPLVVTKLEPL 775
           P  L F++  +K S+ V      +          FG L W    H VR P+++T L PL
Sbjct: 740 PKTLNFTSKHQKLSYTVTFRTRFRMKR-----PEFGGLVWKSTTHKVRSPVIITWLPPL 780

BLAST of CmaCh02G002470 vs. NCBI nr
Match: gi|449458602|ref|XP_004147036.1| (PREDICTED: subtilisin-like protease SBT1.6 [Cucumis sativus])

HSP 1 Score: 1377.8 bits (3565), Expect = 0.0e+00
Identity = 690/774 (89.15%), Postives = 730/774 (94.32%), Query Frame = 1

Query: 1   MAACFATSLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTSEFA 60
           MAA  +TSLSS  FFS LLL+  STV +  PLKTFI R+DRF+KPSVFPTH+HWYTSEF 
Sbjct: 1   MAAFLSTSLSS-FFFSFLLLL--STVSSHSPLKTFIVRIDRFSKPSVFPTHYHWYTSEFT 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           +S +ILHVYDTVFHGFSAT TQ+Q DS+GKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  QSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGI PERRSFSDVNLGPIPRRWKGVC+TGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAG+NA GPIIGINDT+EYRSPRDADGHGTHTASTAAGRH+FQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFD+AVNDGVDVISISIGGGDGVS+PYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIG+YGAAS+G+FVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFP+VV +GN R
Sbjct: 301 DPIAIGSYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGNGR 360

Query: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420
           KI+GVSLYAGAPLNGTMYPLVYPGKSG LS SLCME+SLDPK+VTGKIVICDRGSSPRVA
Sbjct: 361 KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAH+LPACAVGS EGDAMKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           F+GT IGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTD VGPTGLDFD RK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDKRK 540

Query: 541 IEFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEESTGK 600
            EFNILSGTSMACPH+SGAAALLKSAH DWSPAA+RSAMMTTA ITDNR QP TEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKCPARKP 660
            STPYDFGAGHVNLGLAMDPGL+YDITNTDY+NFLCSIGYG KMIQVI+RTPV+CP +KP
Sbjct: 601 PSTPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKP 660

Query: 661 LPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVKPSKLV 720
           LP NLNYPSIV VFSSLSK  STKSFIRTATNVG +NSVY+ KIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVTVFSSLSKGWSTKSFIRTATNVGPSNSVYRVKIEAPKGVTVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPLVVTKLEPL 775
           FST VKKQSFVVAISA+NQNL LG+VGAVFG LSWSDGKHVVR PLVVT+LEPL
Sbjct: 721 FSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of CmaCh02G002470 vs. NCBI nr
Match: gi|659115683|ref|XP_008457681.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 1374.8 bits (3557), Expect = 0.0e+00
Identity = 690/774 (89.15%), Postives = 728/774 (94.06%), Query Frame = 1

Query: 1   MAACFATSLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTSEFA 60
           MAA  +TSLS   FFS LLL+  STV +  PLKTFI R+DRF+KPSVFPTH+HWYTSEF 
Sbjct: 1   MAAFLSTSLSP-FFFSFLLLL--STVSSLSPLKTFIVRIDRFSKPSVFPTHYHWYTSEFT 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           +S +ILHVYDTVFHGFSAT TQEQ DS+GKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  QSPQILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGI PERRSFSDVNLGPIPRRWKGVC+TGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAG+NA GPIIGINDT+EYRSPRDADGHGTHTASTAAGRH+FQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFD+AVNDGVDVISISIGGGDGVS+PYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAAS+G+FVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFP+VV +GN R
Sbjct: 301 DPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGNGR 360

Query: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420
           KI+GVSLYAGAPLNGTMYPLVYPGKSG LS SLCME+SLDPK+VTGKIVICDRGSSPRVA
Sbjct: 361 KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAH+LPACAVGS EGDAMKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           F+GT IGIKPAPVVASFSARGPNGL PEILKPD+IAPGVNILAAWTD VGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLTPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 541 IEFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEESTGK 600
            EFNILSGTSMACPH+SGAAALLKSAH DWSPAA+RSAMMTTA I DNR QP TEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASIIDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKCPARKP 660
            STPYDFGAGHVNLGLAMDPGL+YDITNTDY+NFLCSIGYG KMIQVI+RTPV+CP +KP
Sbjct: 601 PSTPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKP 660

Query: 661 LPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVKPSKLV 720
           LP NLNYPSIVAVFSSLSK  STKSFIRT TNVG +NSVY+ KIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPSNSVYRVKIEAPKGVTVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPLVVTKLEPL 775
           FST VKKQSFVVAISA+NQNL LG+VGAVFG LSWSDGKHVVR PLVVT+LEPL
Sbjct: 721 FSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of CmaCh02G002470 vs. NCBI nr
Match: gi|1009151104|ref|XP_015893374.1| (PREDICTED: subtilisin-like protease SBT1.6 isoform X1 [Ziziphus jujuba])

HSP 1 Score: 1229.2 bits (3179), Expect = 0.0e+00
Identity = 612/777 (78.76%), Postives = 687/777 (88.42%), Query Frame = 1

Query: 6   ATSLSSSIFFSLLLLI-----HGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTSEFA 65
           AT LS+S+ F  L           ++ +D  LKTFIFRVD  +KPSVFPTH+HWYT+EFA
Sbjct: 2   ATLLSASLSFFFLFFFCNFVPQTLSLSSDQNLKTFIFRVDSHSKPSVFPTHYHWYTTEFA 61

Query: 66  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 125
           +  +ILHVYDTVFHGFSAT   +Q  ++ KHPSVLAVFED+RRQLHTTRSPQFLGLRNQR
Sbjct: 62  DPPQILHVYDTVFHGFSATLNPDQVAAITKHPSVLAVFEDQRRQLHTTRSPQFLGLRNQR 121

Query: 126 GLWSDSDYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 185
           GLWSDSDYGSDVI+GVFDTGI PERRSFSD+NLGP+P RWKGVC+TG KFTAKNCNRKIV
Sbjct: 122 GLWSDSDYGSDVIVGVFDTGISPERRSFSDLNLGPVPARWKGVCETGVKFTAKNCNRKIV 181

Query: 186 GARFFSKGHEAGSNAD---GPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEG 245
           GARFF++GHEA +  +   GPI GIN+T E+RSPRDADGHGTHTASTAAGR+AF+AS+EG
Sbjct: 182 GARFFARGHEAAAAVNSIGGPISGINETGEFRSPRDADGHGTHTASTAAGRYAFKASMEG 241

Query: 246 YASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTP 305
           YASGIAKGVAPKARLA+YKVCWKNSGCFDSDILAAFD+AV+DGVDVISISIGGGDG+S+P
Sbjct: 242 YASGIAKGVAPKARLAIYKVCWKNSGCFDSDILAAFDAAVHDGVDVISISIGGGDGISSP 301

Query: 306 YYLDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMG 365
           YYLDPIAIGAYGA SRG+FVSSSAGNDGP GMSVTNLAPW+TTVGAGTIDRNFPAVVI+G
Sbjct: 302 YYLDPIAIGAYGANSRGVFVSSSAGNDGPTGMSVTNLAPWLTTVGAGTIDRNFPAVVILG 361

Query: 366 NRRKIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSP 425
           + RK+ GVSLYAGAPL G MYPLVYPGKSG LSASLCME+SLDPK+V GKIVICDRGSSP
Sbjct: 362 DGRKLTGVSLYAGAPLKGKMYPLVYPGKSGLLSASLCMENSLDPKLVRGKIVICDRGSSP 421

Query: 426 RVAKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTA 485
           RVAKGLVVKKAGGVGMILANG SNGEGLVGDAHI+ ACA+GS EGDA+KAY SS+ +PTA
Sbjct: 422 RVAKGLVVKKAGGVGMILANGASNGEGLVGDAHIIAACAIGSDEGDALKAYLSSTSSPTA 481

Query: 486 TIAFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFD 545
           TI F+GT IGIKPAPVVASFS RGPNGLNPEILKPD+IAPGVNILAAWTD VGPTGLD D
Sbjct: 482 TIDFQGTVIGIKPAPVVASFSGRGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDSD 541

Query: 546 TRKIEFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEES 605
            RK EFNILSGTSMACPH+SGAAALLKSAH DWSPAAIRSAMMTTA   +N+ Q  TEES
Sbjct: 542 LRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAAIRSAMMTTASTVNNQNQMMTEES 601

Query: 606 TGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKCPA 665
           TGK +TPYDFGAGHVNL LAMDPGLVYDITN DYVNFLCSIGYG K+IQVI+R+PV CPA
Sbjct: 602 TGKPATPYDFGAGHVNLDLAMDPGLVYDITNDDYVNFLCSIGYGPKVIQVITRSPVHCPA 661

Query: 666 RKPLPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVKPS 725
           ++PLP NLNYPSI A+FSS +   S+K+FIRT TNVG  N+VY+  ++APKGVTV VKPS
Sbjct: 662 KRPLPENLNYPSIGALFSSAAVGKSSKTFIRTVTNVGEPNAVYRVGVQAPKGVTVTVKPS 721

Query: 726 KLVFSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPLVVTKLEPL 775
           KLVFS AVKK+SF V ++A+++NL LG+ GAVFGS SW+DGKHVVR P+VVT+++PL
Sbjct: 722 KLVFSPAVKKRSFFVTVTADSRNLALGDTGAVFGSFSWTDGKHVVRSPIVVTQIDPL 778

BLAST of CmaCh02G002470 vs. NCBI nr
Match: gi|1009171764|ref|XP_015866916.1| (PREDICTED: subtilisin-like protease SBT1.6 [Ziziphus jujuba])

HSP 1 Score: 1228.8 bits (3178), Expect = 0.0e+00
Identity = 612/777 (78.76%), Postives = 686/777 (88.29%), Query Frame = 1

Query: 6   ATSLSSSIFFSLLLLI-----HGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTSEFA 65
           AT LS+S+ F  L           ++ +D  LKTFIFRVD  +KPSVFPTH+HWYT+EFA
Sbjct: 2   ATLLSASLSFFFLFFFCNFVPQTLSLSSDQNLKTFIFRVDSHSKPSVFPTHYHWYTTEFA 61

Query: 66  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 125
           +  +ILHVYDTVFHGFSAT   +Q  ++ KHPSVLAVFED+RRQLHTTRSPQFLGLRNQR
Sbjct: 62  DPPQILHVYDTVFHGFSATLNPDQVAAITKHPSVLAVFEDQRRQLHTTRSPQFLGLRNQR 121

Query: 126 GLWSDSDYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 185
           GLWSDSDYGSDV  GVFDTGI PERRSFSD+NLGP+P RWKGVC+TG KFTAKNCNRKIV
Sbjct: 122 GLWSDSDYGSDVFAGVFDTGISPERRSFSDLNLGPVPARWKGVCETGVKFTAKNCNRKIV 181

Query: 186 GARFFSKGHEAGSNAD---GPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEG 245
           GARFF++GHEA +  +   GPI GIN+TVE+RSPRDADGHGTHTASTAAGR+AF+AS+EG
Sbjct: 182 GARFFARGHEAAAAVNSIGGPISGINETVEFRSPRDADGHGTHTASTAAGRYAFKASMEG 241

Query: 246 YASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTP 305
           YASGIAKGVAPKARLA+YKVCWKNSGCFDSDILAAFD+AV+DGVDVISISIGGGDG+S+P
Sbjct: 242 YASGIAKGVAPKARLAIYKVCWKNSGCFDSDILAAFDAAVHDGVDVISISIGGGDGISSP 301

Query: 306 YYLDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMG 365
           YYLDPIAIGAYGA SRG+FVSSSAGNDGP GMSVTNLAPW+TTVGAGTIDRNFPAVVI+G
Sbjct: 302 YYLDPIAIGAYGANSRGVFVSSSAGNDGPTGMSVTNLAPWLTTVGAGTIDRNFPAVVILG 361

Query: 366 NRRKIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSP 425
           + RK+ GVSLYAGAPL G MYPLVYPGKSG LSASLCME+SLDPK+V GKIVICDRGSSP
Sbjct: 362 DGRKLTGVSLYAGAPLKGKMYPLVYPGKSGLLSASLCMENSLDPKLVRGKIVICDRGSSP 421

Query: 426 RVAKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTA 485
           RVAKGLVVKKAGGVGMILANG SNGEGLVGDAHI+ ACA+GS EGDA+KAY SS+ +PTA
Sbjct: 422 RVAKGLVVKKAGGVGMILANGASNGEGLVGDAHIIAACAIGSDEGDALKAYLSSTSSPTA 481

Query: 486 TIAFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFD 545
           TI F+GT IGIKPAPVVASFS RGPNGLNPEILKPD+IAPGVNILAAWTD VGPTGLD D
Sbjct: 482 TIDFQGTVIGIKPAPVVASFSGRGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDSD 541

Query: 546 TRKIEFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEES 605
            RK EFNILSGTSMACPH+SGAAALLKSAH DWSPAAIRSAMMTTA   +N+ Q  TEES
Sbjct: 542 LRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAAIRSAMMTTASTVNNQNQMMTEES 601

Query: 606 TGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKCPA 665
           TGK +TPYDFGAGHVNL LAMDPGLVYDITN DYVNFLCSIGYG K+IQVI+R+PV CPA
Sbjct: 602 TGKPATPYDFGAGHVNLDLAMDPGLVYDITNDDYVNFLCSIGYGPKVIQVITRSPVHCPA 661

Query: 666 RKPLPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVKPS 725
           ++PLP NLNYPSI A+FSS +   S+K+FIRT TNVG  N+VY+  ++APKGVTV VKPS
Sbjct: 662 KRPLPENLNYPSIGALFSSAAVGKSSKTFIRTVTNVGEPNAVYRVGVQAPKGVTVTVKPS 721

Query: 726 KLVFSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPLVVTKLEPL 775
           KLVFS AVKK+SF V ++A+++NL LG+ GAVFGS SW+DGKHVVR P+VVT+++PL
Sbjct: 722 KLVFSPAVKKRSFFVTVTADSRNLALGDTGAVFGSFSWTDGKHVVRSPIVVTQIDPL 778

BLAST of CmaCh02G002470 vs. NCBI nr
Match: gi|703076406|ref|XP_010090327.1| (Subtilisin-like protease [Morus notabilis])

HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 590/743 (79.41%), Postives = 668/743 (89.91%), Query Frame = 1

Query: 32  LKTFIFRVDRFAKPSVFPTHFHWYTSEFAESHKILHVYDTVFHGFSATFTQEQADSVGKH 91
           LKTFIFRVD   KPS+FPTH+HWYT+EFA+  +ILHVYDTVF+GFSA  + +Q     +H
Sbjct: 36  LKTFIFRVDSHTKPSIFPTHYHWYTTEFADPPQILHVYDTVFNGFSAVLSSDQVAYASRH 95

Query: 92  PSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGICPERRSFSDV 151
           PSVLAVFED+RRQLHTTRSPQFLGLRNQRGLWS+SDYGSDVIIGVFDTGI PERRSFSD+
Sbjct: 96  PSVLAVFEDKRRQLHTTRSPQFLGLRNQRGLWSESDYGSDVIIGVFDTGIWPERRSFSDL 155

Query: 152 NLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGHEAGSNADGPIIGINDTVEYRSP 211
           NLGPIP RWKGVC++G KF+ +NCNRK++GARFFSKGHEA  +  GPI G+NDT+E+RSP
Sbjct: 156 NLGPIPSRWKGVCESGAKFSVRNCNRKLIGARFFSKGHEAAGSIGGPISGVNDTLEFRSP 215

Query: 212 RDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILA 271
           RDADGHGTHTASTAAGR+AF+AS+ GYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILA
Sbjct: 216 RDADGHGTHTASTAAGRYAFEASMAGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILA 275

Query: 272 AFDSAVNDGVDVISISIGGGDGVSTPYYLDPIAIGAYGAASRGIFVSSSAGNDGPNGMSV 331
           AFD+AVNDGVDVISISIGGGDG+S+PYYLDPIAIGAYGA S+G+FVSSSAGNDGPNGMSV
Sbjct: 276 AFDAAVNDGVDVISISIGGGDGISSPYYLDPIAIGAYGAVSKGVFVSSSAGNDGPNGMSV 335

Query: 332 TNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYAGAPLNGTMYPLVYPGKSGELSA 391
           TNLAPW+TTVGAGTIDR FPAV+++G+ R++ GVSLYAGAPL G MYPLVYPGKSG L A
Sbjct: 336 TNLAPWMTTVGAGTIDRTFPAVIVLGDGRRLSGVSLYAGAPLKGKMYPLVYPGKSGMLPA 395

Query: 392 SLCMESSLDPKIVTGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHI 451
           SLCME+SLDP +V GKIVICDRGSSPRVAKG+VVKKAGGVGMIL+NGIS G GLVGDAHI
Sbjct: 396 SLCMENSLDPNLVRGKIVICDRGSSPRVAKGMVVKKAGGVGMILSNGISQGGGLVGDAHI 455

Query: 452 LPACAVGSVEGDAMKAYASSSRNPTATIAFRGTRIGIKPAPVVASFSARGPNGLNPEILK 511
           LPACAVGS EG+A+KAY SS+ NPTATI F+GT IGIKPAP+VASFS RGPN +NPEILK
Sbjct: 456 LPACAVGSDEGNAVKAYVSSASNPTATIDFQGTVIGIKPAPIVASFSGRGPNSVNPEILK 515

Query: 512 PDMIAPGVNILAAWTDVVGPTGLDFDTRKIEFNILSGTSMACPHISGAAALLKSAHLDWS 571
           PD+IAPGVNILAAWTD VGPTGLD D RK EFNILSGTSMACPH+SGAAALLKSAH DWS
Sbjct: 516 PDLIAPGVNILAAWTDAVGPTGLDSDKRKTEFNILSGTSMACPHVSGAAALLKSAHPDWS 575

Query: 572 PAAIRSAMMTTAKITDNRWQPTTEESTGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDY 631
           PAAIRSAMMTTA I DNR Q  T+ESTGK+STPYD GAGH+NL  AMDPGLVYDITN D+
Sbjct: 576 PAAIRSAMMTTASIVDNRNQLMTDESTGKSSTPYDLGAGHLNLDRAMDPGLVYDITNDDH 635

Query: 632 VNFLCSIGYGAKMIQVISRTPVKCPARKPLPGNLNYPSIVAVFSSLSKVLSTKSFIRTAT 691
           VNFLCSIGYG K+IQVI+RTPVKCP ++PLP NLNYPS+ A+F + S+  ++K FIRT T
Sbjct: 636 VNFLCSIGYGPKVIQVITRTPVKCPVKRPLPENLNYPSMAALFPTSSRGSTSKMFIRTVT 695

Query: 692 NVGAANSVYQAKIEAPKGVTVKVKPSKLVFSTAVKKQSFVVAISANNQNLTLGNVGAVFG 751
           NVGA NSVY+A+IEAPKGVTV+VKP+KLVF+ AVKKQSFVV ++A+ ++L LG  GA FG
Sbjct: 696 NVGAPNSVYRARIEAPKGVTVRVKPAKLVFTEAVKKQSFVVTVTADARSLVLGESGANFG 755

Query: 752 SLSWSDGKHVVRMPLVVTKLEPL 775
           SLSW+DGKHVVR P+VVT+++PL
Sbjct: 756 SLSWTDGKHVVRSPIVVTEIQPL 778