Carg05167 (gene) Silver-seed gourd

NameCarg05167
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionSubtilisin-like protease
LocationCucurbita_argyrosperma_scaffold_010 : 245515 .. 247839 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCTTGTTTCGCCGCTTCTCTCTCCTCTTCCATCTTCTTCTCTCTCCTCCTGTTAATCCATGGCTCTACAGTTCCGGGTGATCCGCCATTGAAGACCTTCATCTTCCGTGTGGACAGTTTCTCTAAACCCTCTGTTTTTCCCACCCATTTCCACTGGTACACTTCCGAGTTCGCCGAATCCCACAAAATCCTCCATGTTTACGACACCGTGTTTCATGGATTCTCTGCAACTTTCACTCAAGAGCAAGCCGATTCCGTCGGAAAACACCCTTCTGTGCTTGCTGTCTTTGAGGACCGTCGCCGTCAGCTTCACACCACGCGTTCCCCTCAATTTCTCGGCCTTCGAAACCAGCGTGGTTTGTGGTCTGATTCCGATTATGGGTCGGATGTAATCATTGGAGTTTTCGATACTGGGATTTCCCCTGAACGGCGGAGCTTCTCCGATGTGAATTTGGGGCCGATTCCTCGCCGGTGGAAAGGGGTTTGTCAGACTGGAACTAAGTTCACGGCCAAAAACTGTAATAGGAAGATCGTCGGTGCGAGGTTTTTCTCTAAGGGTCACGAAGCAGGGGCCAACGCTGCTGGTCCGATTATTGGGATTAACGACACCGTTGAGTACCGATCTCCGAGAGACGCCGACGGCCATGGGACTCACACGGCATCGACGGCGGCTGGGCGTCACGCGTTTCAGGCGAGTTTGGAGGGTTATGCTTCGGGAATCGCGAAGGGTGTAGCCCCTAAGGCACGTTTGGCGGTTTACAAAGTTTGTTGGAAAAATTCGGGTTGTTTCGATTCCGATATTCTCGCGGCGTTTGATTCTGCTGTTAAGGACGGCGTCGATGTCATTTCGATCTCGATAGGTGGCGGCGATGGCGTTTCCACGCCGTATTATCTTGACCCAATTGCAATCGGGGCGTACGGCGCCGCTTCTAGGGGCATTTTCGTTTCGTCTTCGGCTGGCAATGATGGACCCAATGGGATGTCAGTAACGAACTTAGCGCCGTGGATTACGACGGTCGGAGCTGGTACGATTGACCGGAATTTCCCGGCGGTGGTGATTATGGGAAACCGACGGAAGATTCACGGCGTTTCTCTGTACGCCGGAGCGCCATTGAACAGTACAATGTATCCGTTGGTTTACCCTGGAAAATCAGGGGAGCTCTCTGCTTCACTCTGTATGGAGAGTTCGCTTGACCCTAAGATTGTGACCGGAAAAATTGTTATCTGTGACAGAGGAAGTAGCCCGAGAGTGGAGAAAGGTTTGGTGGTGAAGAAGGCCGGCGGCGTCGGAATGATTTTGGCGAACGGAATTTCAAACGGCGAAGGACTCGTCGGTGATGCCCATATCCTTCCCGCCTGCGCCGTTGGCTCTGTAGAAGGCGATGCCATGAAAGCCTACGCATCGTCCTCTGGAAATCCCACCGCCACCATCGCCTTCCGAGGCACAAGAATCGGAATCAAACCGGCGCCGGTGGTGGCTTCGTTTTCTGCAAGAGGGCCAAATGGATTAAACCCTGAAATTCTAAAACCGGACATGATCGCACCAGGGGTTAACATTTTAGCTGCTTGGACAGATGTCGTCGGCCCAACCGGTTTAGACTTCGATACACGAAAAACAGAGTTCAATATCTTGTCAGGTACATCAATGGCGTGTCCTCATATAAGTGGAGCCGCCGCTCTGTTGAAATCAGCACATCCCGATTGGAGTCCGGCGGCGGTAAGATCCGCCATGATGACCACCGCAAGAATCACTGACAACCGACGGCAGCCGACGACGGAAGAGTCCACCGGAAAAGCTTCTACACCGTATGATTTTGGTGCCGGTCATGTGAATCTCGGCCTCGCAATGGACCCTGGACTAGTCTACGACATTACAAACACCGATTATGTCAACTTCCTATGCTCAATCGGCTACGGAACGAAGATGATCCAAGTCATATCTCGAACGCCGGTGAAATGTCCGGCGAGGAAGCCATTGCCAGAGAATTTGAACTACCCATCAATCGTAGCCGTGTTTTCCAGTCTATCAAAAGGGTTGTCCACAAAATCGTTTATTCGAACGGCGACGAACGTGGGGGCGGCGAATTCGGTGTACCAAGCTAAAATCGAAGCTCCGAAAGGGGCAACGGTAAAGGTAAAGCCGTCGAAACTAGTATTCTCAACGGCGGTAAAGAAACAGAGCTTCGTAGTGGCCATATCAGCCAATGACCAGAATCTGACACTAGGCGATGTGGGTGCAGTCTTTGGTTCTCTTTCTTGGTCTGATGGAAAGCATGTGGTCCGAACACCACTGGTAGTTACTAAATTAGAGCCGTTGTAG

mRNA sequence

ATGGCCGCTTGTTTCGCCGCTTCTCTCTCCTCTTCCATCTTCTTCTCTCTCCTCCTGTTAATCCATGGCTCTACAGTTCCGGGTGATCCGCCATTGAAGACCTTCATCTTCCGTGTGGACAGTTTCTCTAAACCCTCTGTTTTTCCCACCCATTTCCACTGGTACACTTCCGAGTTCGCCGAATCCCACAAAATCCTCCATGTTTACGACACCGTGTTTCATGGATTCTCTGCAACTTTCACTCAAGAGCAAGCCGATTCCGTCGGAAAACACCCTTCTGTGCTTGCTGTCTTTGAGGACCGTCGCCGTCAGCTTCACACCACGCGTTCCCCTCAATTTCTCGGCCTTCGAAACCAGCGTGGTTTGTGGTCTGATTCCGATTATGGGTCGGATGTAATCATTGGAGTTTTCGATACTGGGATTTCCCCTGAACGGCGGAGCTTCTCCGATGTGAATTTGGGGCCGATTCCTCGCCGGTGGAAAGGGGTTTGTCAGACTGGAACTAAGTTCACGGCCAAAAACTGTAATAGGAAGATCGTCGGTGCGAGGTTTTTCTCTAAGGGTCACGAAGCAGGGGCCAACGCTGCTGGTCCGATTATTGGGATTAACGACACCGTTGAGTACCGATCTCCGAGAGACGCCGACGGCCATGGGACTCACACGGCATCGACGGCGGCTGGGCGTCACGCGTTTCAGGCGAGTTTGGAGGGTTATGCTTCGGGAATCGCGAAGGGTGTAGCCCCTAAGGCACGTTTGGCGGTTTACAAAGTTTGTTGGAAAAATTCGGGTTGTTTCGATTCCGATATTCTCGCGGCGTTTGATTCTGCTGTTAAGGACGGCGTCGATGTCATTTCGATCTCGATAGGTGGCGGCGATGGCGTTTCCACGCCGTATTATCTTGACCCAATTGCAATCGGGGCGTACGGCGCCGCTTCTAGGGGCATTTTCGTTTCGTCTTCGGCTGGCAATGATGGACCCAATGGGATGTCAGTAACGAACTTAGCGCCGTGGATTACGACGGTCGGAGCTGGTACGATTGACCGGAATTTCCCGGCGGTGGTGATTATGGGAAACCGACGGAAGATTCACGGCGTTTCTCTGTACGCCGGAGCGCCATTGAACAGTACAATGTATCCGTTGGTTTACCCTGGAAAATCAGGGGAGCTCTCTGCTTCACTCTGTATGGAGAGTTCGCTTGACCCTAAGATTGTGACCGGAAAAATTGTTATCTGTGACAGAGGAAGTAGCCCGAGAGTGGAGAAAGGTTTGGTGGTGAAGAAGGCCGGCGGCGTCGGAATGATTTTGGCGAACGGAATTTCAAACGGCGAAGGACTCGTCGGTGATGCCCATATCCTTCCCGCCTGCGCCGTTGGCTCTGTAGAAGGCGATGCCATGAAAGCCTACGCATCGTCCTCTGGAAATCCCACCGCCACCATCGCCTTCCGAGGCACAAGAATCGGAATCAAACCGGCGCCGGTGGTGGCTTCGTTTTCTGCAAGAGGGCCAAATGGATTAAACCCTGAAATTCTAAAACCGGACATGATCGCACCAGGGGTTAACATTTTAGCTGCTTGGACAGATGTCGTCGGCCCAACCGGTTTAGACTTCGATACACGAAAAACAGAGTTCAATATCTTGTCAGGTACATCAATGGCGTGTCCTCATATAAGTGGAGCCGCCGCTCTGTTGAAATCAGCACATCCCGATTGGAGTCCGGCGGCGGTAAGATCCGCCATGATGACCACCGCAAGAATCACTGACAACCGACGGCAGCCGACGACGGAAGAGTCCACCGGAAAAGCTTCTACACCGTATGATTTTGGTGCCGGTCATGTGAATCTCGGCCTCGCAATGGACCCTGGACTAGTCTACGACATTACAAACACCGATTATGTCAACTTCCTATGCTCAATCGGCTACGGAACGAAGATGATCCAAGTCATATCTCGAACGCCGGTGAAATGTCCGGCGAGGAAGCCATTGCCAGAGAATTTGAACTACCCATCAATCGTAGCCGTGTTTTCCAGTCTATCAAAAGGGTTGTCCACAAAATCGTTTATTCGAACGGCGACGAACGTGGGGGCGGCGAATTCGGTGTACCAAGCTAAAATCGAAGCTCCGAAAGGGGCAACGGTAAAGGTAAAGCCGTCGAAACTAGTATTCTCAACGGCGGTAAAGAAACAGAGCTTCGTAGTGGCCATATCAGCCAATGACCAGAATCTGACACTAGGCGATGTGGGTGCAGTCTTTGGTTCTCTTTCTTGGTCTGATGGAAAGCATGTGGTCCGAACACCACTGGTAGTTACTAAATTAGAGCCGTTGTAG

Coding sequence (CDS)

ATGGCCGCTTGTTTCGCCGCTTCTCTCTCCTCTTCCATCTTCTTCTCTCTCCTCCTGTTAATCCATGGCTCTACAGTTCCGGGTGATCCGCCATTGAAGACCTTCATCTTCCGTGTGGACAGTTTCTCTAAACCCTCTGTTTTTCCCACCCATTTCCACTGGTACACTTCCGAGTTCGCCGAATCCCACAAAATCCTCCATGTTTACGACACCGTGTTTCATGGATTCTCTGCAACTTTCACTCAAGAGCAAGCCGATTCCGTCGGAAAACACCCTTCTGTGCTTGCTGTCTTTGAGGACCGTCGCCGTCAGCTTCACACCACGCGTTCCCCTCAATTTCTCGGCCTTCGAAACCAGCGTGGTTTGTGGTCTGATTCCGATTATGGGTCGGATGTAATCATTGGAGTTTTCGATACTGGGATTTCCCCTGAACGGCGGAGCTTCTCCGATGTGAATTTGGGGCCGATTCCTCGCCGGTGGAAAGGGGTTTGTCAGACTGGAACTAAGTTCACGGCCAAAAACTGTAATAGGAAGATCGTCGGTGCGAGGTTTTTCTCTAAGGGTCACGAAGCAGGGGCCAACGCTGCTGGTCCGATTATTGGGATTAACGACACCGTTGAGTACCGATCTCCGAGAGACGCCGACGGCCATGGGACTCACACGGCATCGACGGCGGCTGGGCGTCACGCGTTTCAGGCGAGTTTGGAGGGTTATGCTTCGGGAATCGCGAAGGGTGTAGCCCCTAAGGCACGTTTGGCGGTTTACAAAGTTTGTTGGAAAAATTCGGGTTGTTTCGATTCCGATATTCTCGCGGCGTTTGATTCTGCTGTTAAGGACGGCGTCGATGTCATTTCGATCTCGATAGGTGGCGGCGATGGCGTTTCCACGCCGTATTATCTTGACCCAATTGCAATCGGGGCGTACGGCGCCGCTTCTAGGGGCATTTTCGTTTCGTCTTCGGCTGGCAATGATGGACCCAATGGGATGTCAGTAACGAACTTAGCGCCGTGGATTACGACGGTCGGAGCTGGTACGATTGACCGGAATTTCCCGGCGGTGGTGATTATGGGAAACCGACGGAAGATTCACGGCGTTTCTCTGTACGCCGGAGCGCCATTGAACAGTACAATGTATCCGTTGGTTTACCCTGGAAAATCAGGGGAGCTCTCTGCTTCACTCTGTATGGAGAGTTCGCTTGACCCTAAGATTGTGACCGGAAAAATTGTTATCTGTGACAGAGGAAGTAGCCCGAGAGTGGAGAAAGGTTTGGTGGTGAAGAAGGCCGGCGGCGTCGGAATGATTTTGGCGAACGGAATTTCAAACGGCGAAGGACTCGTCGGTGATGCCCATATCCTTCCCGCCTGCGCCGTTGGCTCTGTAGAAGGCGATGCCATGAAAGCCTACGCATCGTCCTCTGGAAATCCCACCGCCACCATCGCCTTCCGAGGCACAAGAATCGGAATCAAACCGGCGCCGGTGGTGGCTTCGTTTTCTGCAAGAGGGCCAAATGGATTAAACCCTGAAATTCTAAAACCGGACATGATCGCACCAGGGGTTAACATTTTAGCTGCTTGGACAGATGTCGTCGGCCCAACCGGTTTAGACTTCGATACACGAAAAACAGAGTTCAATATCTTGTCAGGTACATCAATGGCGTGTCCTCATATAAGTGGAGCCGCCGCTCTGTTGAAATCAGCACATCCCGATTGGAGTCCGGCGGCGGTAAGATCCGCCATGATGACCACCGCAAGAATCACTGACAACCGACGGCAGCCGACGACGGAAGAGTCCACCGGAAAAGCTTCTACACCGTATGATTTTGGTGCCGGTCATGTGAATCTCGGCCTCGCAATGGACCCTGGACTAGTCTACGACATTACAAACACCGATTATGTCAACTTCCTATGCTCAATCGGCTACGGAACGAAGATGATCCAAGTCATATCTCGAACGCCGGTGAAATGTCCGGCGAGGAAGCCATTGCCAGAGAATTTGAACTACCCATCAATCGTAGCCGTGTTTTCCAGTCTATCAAAAGGGTTGTCCACAAAATCGTTTATTCGAACGGCGACGAACGTGGGGGCGGCGAATTCGGTGTACCAAGCTAAAATCGAAGCTCCGAAAGGGGCAACGGTAAAGGTAAAGCCGTCGAAACTAGTATTCTCAACGGCGGTAAAGAAACAGAGCTTCGTAGTGGCCATATCAGCCAATGACCAGAATCTGACACTAGGCGATGTGGGTGCAGTCTTTGGTTCTCTTTCTTGGTCTGATGGAAAGCATGTGGTCCGAACACCACTGGTAGTTACTAAATTAGAGCCGTTGTAG

Protein sequence

MAACFAASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFAESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVEKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGTKMIQVISRTPVKCPARKPLPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLVFSTAVKKQSFVVAISANDQNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL
BLAST of Carg05167 vs. NCBI nr
Match: XP_022946948.1 (subtilisin-like protease SBT1.6 [Cucurbita moschata])

HSP 1 Score: 1518.4 bits (3930), Expect = 0.0e+00
Identity = 768/774 (99.22%), Postives = 768/774 (99.22%), Query Frame = 0

Query: 1   MAACFAASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60
           MAACF ASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA
Sbjct: 1   MAACFDASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360

Query: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420
           KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE
Sbjct: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK
Sbjct: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
           TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK
Sbjct: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGTKMIQVISRTPVKCPARKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYG KMIQVISRT VKCPARKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPARKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV 720
           LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKL 
Sbjct: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLA 720

Query: 721 FSTAVKKQSFVVAISANDQNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           FSTAVKKQSFVVAISAN  NLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL
Sbjct: 721 FSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 774

BLAST of Carg05167 vs. NCBI nr
Match: XP_023532451.1 (subtilisin-like protease SBT1.6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1508.0 bits (3903), Expect = 0.0e+00
Identity = 762/774 (98.45%), Postives = 765/774 (98.84%), Query Frame = 0

Query: 1   MAACFAASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60
           MAACFAASLSSSIFFSLLLLIHGSTVP DPPLKTFIFRVDSF+KPSVFPTHFHWYTSEFA
Sbjct: 1   MAACFAASLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFA 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTV HGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAV DGVDVISISIGGGDGVSTPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360

Query: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420
           KIHGVSLYAGAPLN TMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 
Sbjct: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK
Sbjct: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
           TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK
Sbjct: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGTKMIQVISRTPVKCPARKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGY +KMIQVISRTPVKCP RKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKCPTRKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV 720
           LP NLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV
Sbjct: 661 LPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANDQNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           FSTAVKKQSFVVAISAN+QNLTLGDVGAVFGSL WSDGKHVVRTPLVVTKLEPL
Sbjct: 721 FSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL 774

BLAST of Carg05167 vs. NCBI nr
Match: XP_022970938.1 (subtilisin-like protease SBT1.6 [Cucurbita maxima])

HSP 1 Score: 1485.3 bits (3844), Expect = 0.0e+00
Identity = 751/774 (97.03%), Postives = 757/774 (97.80%), Query Frame = 0

Query: 1   MAACFAASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60
           MAACFA SLSSSIFFSLLLLIHGSTVP DPPLKTFIFRVD F+KPSVFPTHFHWYTSEFA
Sbjct: 1   MAACFATSLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTSEFA 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGI PERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAG+NA GPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS
Sbjct: 181 GARFFSKGHEAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAV DGVDVISISIGGGDGVSTPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360

Query: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420
           KIHGVSLYAGAPLN TMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 
Sbjct: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK
Sbjct: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
            EFNILSGTSMACPHISGAAALLKSAH DWSPAA+RSAMMTTA+ITDNR QPTTEESTGK
Sbjct: 541 IEFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGTKMIQVISRTPVKCPARKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYG KMIQVISRTPVKCPARKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKCPARKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV 720
           LP NLNYPSIVAVFSSLSK LSTKSFIRTATNVGAANSVYQAKIEAPKG TVKVKPSKLV
Sbjct: 661 LPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANDQNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           FSTAVKKQSFVVAISAN+QNLTLG+VGAVFGSLSWSDGKHVVR PLVVTKLEPL
Sbjct: 721 FSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPLVVTKLEPL 774

BLAST of Carg05167 vs. NCBI nr
Match: XP_023512905.1 (subtilisin-like protease SBT1.6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1390.2 bits (3597), Expect = 0.0e+00
Identity = 690/770 (89.61%), Postives = 731/770 (94.94%), Query Frame = 0

Query: 6   AASLSSSIFFSLLLLIHGSTVPGDPPL-KTFIFRVDSFSKPSVFPTHFHWYTSEFAESHK 65
           AA LS  +FF  +L++H  TV  D PL KTFIFRVD FSKPSVFPTH+HWY+SEFAESHK
Sbjct: 2   AAYLSPLVFFFFVLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYSSEFAESHK 61

Query: 66  ILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWS 125
           ILHVYDTVFHGFSAT TQ+Q DS+GKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWS
Sbjct: 62  ILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWS 121

Query: 126 DSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARF 185
           DSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTA+NCNRKIVGARF
Sbjct: 122 DSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARF 181

Query: 186 FSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAK 245
           FSKGHEAG+NAAGPIIGINDT+E+RSPRDADGHGTHTASTAAGRHAFQASLEG+ASGIAK
Sbjct: 182 FSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAK 241

Query: 246 GVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDPIA 305
           GVAPKARLAVYKVCWKNSGCFDSDILAAFD+AV DGVDVISISIGGGDGVS+PYYLDPIA
Sbjct: 242 GVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIA 301

Query: 306 IGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHG 365
           IGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVV +GN R+I G
Sbjct: 302 IGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISG 361

Query: 366 VSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVEKGLV 425
           VSLYAGAPLN TM+PLVYPGKSG LS SLCM++SLDPK+V GKIVICDRGSSPRV KGLV
Sbjct: 362 VSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLV 421

Query: 426 VKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAFRGT 485
           VKKAGGVGMILANGISNGEGLVGDAH+LPACAVGS EGD+MKAYASSS NPTATIAF+GT
Sbjct: 422 VKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSANPTATIAFQGT 481

Query: 486 RIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKTEFN 545
            IGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTD VGPTGLDFDTRKTEFN
Sbjct: 482 IIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFN 541

Query: 546 ILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKASTP 605
           ILSGTSMACPH+SGAAALLKSAHPDWSPAA+RSAMMTTA  TDNRRQP TEESTGKASTP
Sbjct: 542 ILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTP 601

Query: 606 YDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGTKMIQVISRTPVKCPARKPLPEN 665
           YDFGAGHVNLGLAMDPGLVYDITNTDY+NFLCSIGYG KMIQVI+RTPV CPA+KPLPEN
Sbjct: 602 YDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPLPEN 661

Query: 666 LNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLVFSTA 725
           LNYPSIVAVFSSLSKG STKSFIRT TNVG ANSVY+AKI+APKG TVKVKPSKLVFS A
Sbjct: 662 LNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIQAPKGVTVKVKPSKLVFSAA 721

Query: 726 VKKQSFVVAISANDQNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           +KKQSFVVA+SA++QNL LGDVGAVFG +SWSDGKHVVR+PLVVT+LEPL
Sbjct: 722 MKKQSFVVAVSADNQNLALGDVGAVFGCVSWSDGKHVVRSPLVVTQLEPL 771

BLAST of Carg05167 vs. NCBI nr
Match: XP_022985703.1 (subtilisin-like protease SBT1.6 [Cucurbita maxima])

HSP 1 Score: 1389.8 bits (3596), Expect = 0.0e+00
Identity = 691/769 (89.86%), Postives = 730/769 (94.93%), Query Frame = 0

Query: 7   ASLSSSIFFSLLLLIHGSTVPGDPPL-KTFIFRVDSFSKPSVFPTHFHWYTSEFAESHKI 66
           A+  S +FF  LL++H  TV  D PL KTFIFRVD FSKPSVFPTH+HWYTSEFAESHKI
Sbjct: 2   AAYLSPLFFFFLLIVHRLTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESHKI 61

Query: 67  LHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSD 126
           LHVYDTVFHGFSAT TQ+Q DS+GKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSD
Sbjct: 62  LHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSD 121

Query: 127 SDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFF 186
           SDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTA+NCNRKIVGARFF
Sbjct: 122 SDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARFF 181

Query: 187 SKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKG 246
           SKGHEAG+NAAGPIIGINDT+E+RSPRDADGHGTHTASTAAGRHAFQASLEG+ASGIAKG
Sbjct: 182 SKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAKG 241

Query: 247 VAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDPIAI 306
           VAPKARLAVYKVCWKNSGCFDSDILAAFD+AV DGVDVISISIGGGDGVS+PYYLDPIAI
Sbjct: 242 VAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAI 301

Query: 307 GAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGV 366
           GAYGAASRGIFVSSS GNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVV +GN R+I GV
Sbjct: 302 GAYGAASRGIFVSSSGGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISGV 361

Query: 367 SLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVEKGLVV 426
           SLYAGAPLN+TM+PLVYPGKSG LS SLCM++SLDPK+V GKIVICDRGSSPRV KGLVV
Sbjct: 362 SLYAGAPLNATMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLVV 421

Query: 427 KKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAFRGTR 486
           KKAGGVGMILANGISNGEGLVGDAH+LPACAVGS EGD+MKAYASSS NPTATIAF+GT 
Sbjct: 422 KKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSANPTATIAFQGTI 481

Query: 487 IGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNI 546
           IGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTD VGPTGLDFDTRKTEFNI
Sbjct: 482 IGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNI 541

Query: 547 LSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKASTPY 606
           LSGTSMACPH+SGAAALLKSAHPDWSPAA+RSAMMTTA  TDNRRQP TEESTGKASTPY
Sbjct: 542 LSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTPY 601

Query: 607 DFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGTKMIQVISRTPVKCPARKPLPENL 666
           DFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYG KMIQVI+RTPV CPA+KPLPENL
Sbjct: 602 DFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGPKMIQVITRTPVTCPAKKPLPENL 661

Query: 667 NYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLVFSTAV 726
           NYPSIVAVFSSLSKG STKSFIRT TNVG ANSVY+AKIEAPKG TVKVKPSKLVFS A+
Sbjct: 662 NYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAAM 721

Query: 727 KKQSFVVAISANDQNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           KKQSFVVA+SA++QNL LGDVGAVFG +SWSDGKHVVR+PLVVT+LEPL
Sbjct: 722 KKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 770

BLAST of Carg05167 vs. TAIR10
Match: AT4G34980.1 (subtilisin-like serine protease 2)

HSP 1 Score: 1142.1 bits (2953), Expect = 0.0e+00
Identity = 556/767 (72.49%), Postives = 658/767 (85.79%), Query Frame = 0

Query: 10  SSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFAESHKILHVY 69
           SS+I   L L     +       KTFIFR+D  S PS+FPTH+HWY++EFAE  +I+HVY
Sbjct: 3   SSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVY 62

Query: 70  DTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYG 129
            TVFHGFSA  T ++AD++  HP+VLAVFEDRRR+LHTTRSPQFLGL+NQ+GLWS+SDYG
Sbjct: 63  HTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYG 122

Query: 130 SDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGH 189
           SDVIIGVFDTGI PERRSFSD+NLGPIP+RW+GVC++G +F+ +NCNRKI+GARFF+KG 
Sbjct: 123 SDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQ 182

Query: 190 EAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPK 249
           +A       I GIN TVE+ SPRDADGHGTHT+STAAGRHAF+AS+ GYASG+AKGVAPK
Sbjct: 183 QAAV-----IGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPK 242

Query: 250 ARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDPIAIGAYG 309
           AR+A YKVCWK+SGC DSDILAAFD+AV+DGVDVISISIGGGDG+++PYYLDPIAIG+YG
Sbjct: 243 ARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYG 302

Query: 310 AASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYA 369
           AAS+GIFVSSSAGN+GPNGMSVTNLAPW+TTVGA TIDRNFPA  I+G+  ++ GVSLYA
Sbjct: 303 AASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYA 362

Query: 370 GAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVEKGLVVKKAG 429
           G PLN  M+P+VYPGKSG  SASLCME++LDPK V GKIVICDRGSSPRV KGLVVKKAG
Sbjct: 363 GVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAG 422

Query: 430 GVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAFRGTRIGIK 489
           GVGMILANG SNGEGLVGDAH++PACAVGS EGD +KAYASS  NP A+I FRGT +GIK
Sbjct: 423 GVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIK 482

Query: 490 PAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNILSGT 549
           PAPV+ASFS RGPNGL+PEILKPD+IAPGVNILAAWTD VGPTGL  D RKTEFNILSGT
Sbjct: 483 PAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGT 542

Query: 550 SMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKASTPYDFGA 609
           SMACPH+SGAAALLKSAHPDWSPA +RSAMMTT  + DN  +   +ESTGK++TPYD+G+
Sbjct: 543 SMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGS 602

Query: 610 GHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGTKMIQVISRTPVKCP-ARKPLPENLNYP 669
           GH+NLG AM+PGLVYDITN DY+ FLCSIGYG K IQVI+RTPV+CP  RKP P NLNYP
Sbjct: 603 GHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYP 662

Query: 670 SIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLVFSTAVKKQ 729
           SI AVF +  +GL +K+ IRTATNVG A +VY+A+IE+P+G TV VKP +LVF++AVK++
Sbjct: 663 SITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRR 722

Query: 730 SFVVAISANDQNLTLGDVGAVFGSLSWSD-GKHVVRTPLVVTKLEPL 775
           S+ V ++ N +N+ LG+ GAVFGS++W D GKHVVR+P+VVT+++ L
Sbjct: 723 SYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

BLAST of Carg05167 vs. TAIR10
Match: AT3G14240.1 (Subtilase family protein)

HSP 1 Score: 838.6 bits (2165), Expect = 3.2e-243
Identity = 437/759 (57.58%), Postives = 538/759 (70.88%), Query Frame = 0

Query: 34  TFIFRVDSFSKPSVFPTHFHWYTSEFA----ESHKILHVYDTVFHGFSATFTQEQADSVG 93
           T+I  VD  +KPS+FPTHFHWYTS  A        I+H YDTVFHGFSA  T + A  + 
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTYDTVFHGFSARLTSQDASQLL 86

Query: 94  KHPSVLAVFEDRRRQLHTTRSPQFLGLR--NQRGLWSDSDYGSDVIIGVFDTGISPERRS 153
            HP V++V  ++ R LHTTRSP+FLGLR  ++ GL  +SD+GSD++IGV DTG+ PER S
Sbjct: 87  DHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTGVWPERPS 146

Query: 154 FSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGHEAGANAAGPIIGINDTVE 213
           F D  LGP+P +WKG C     F    CNRK+VGARFF  G+EA          +N+T E
Sbjct: 147 FDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGK------MNETTE 206

Query: 214 YRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDS 273
           +RSPRD+DGHGTHTAS +AGR+ F AS  GYA G+A G+APKARLA YKVCW NSGC+DS
Sbjct: 207 FRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCW-NSGCYDS 266

Query: 274 DILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDPIAIGAYGAASRGIFVSSSAGNDGPN 333
           DILAAFD+AV DGVDVIS+S+G   GV  PYYLD IAIGA+GA  RGIFVS+SAGN GP 
Sbjct: 267 DILAAFDTAVADGVDVISLSVG---GVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPG 326

Query: 334 GMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYAGAPLN-STMYPLVYPGK- 393
            ++VTN+APW+TTVGAGTIDR+FPA V +GN + I GVS+Y G  L+   MYPLVY G  
Sbjct: 327 ALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSL 386

Query: 394 --SGELSASLCMESSLDPKIVTGKIVICDRGSSPRVEKGLVVKKAGGVGMILANGISNGE 453
                 S+SLC+E SLDP +V GKIV+CDRG + R  KG +V+K GG+GMI+ANG+ +GE
Sbjct: 387 LGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGE 446

Query: 454 GLVGDAHILPACAVGSVEGDAMKAYAS------SSGNPTATIAFRGTRIGIKPAPVVASF 513
           GLV D H+LPA +VG+  GD ++ Y S      SS +PTATI F+GTR+GI+PAPVVASF
Sbjct: 447 GLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASF 506

Query: 514 SARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNILSGTSMACPHIS 573
           SARGPN   PEILKPD+IAPG+NILAAW D +GP+G+  D R+TEFNILSGTSMACPH+S
Sbjct: 507 SARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVS 566

Query: 574 GAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKASTPYDFGAGHVNLGLA 633
           G AALLK+AHPDWSPAA+RSA++TTA   DN  +P  +ESTG  S+  D+G+GHV+   A
Sbjct: 567 GLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKA 626

Query: 634 MDPGLVYDITNTDYVNFLCSIGYGTKMIQVISRTPVKCPARKPLPE--NLNYPSIVAVFS 693
           MDPGLVYDIT+ DY+NFLC+  Y    I  I+R    C   +      NLNYPS   VF 
Sbjct: 627 MDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQ 686

Query: 694 SLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLVFSTAVKKQSFVVAIS 753
              +   +  FIRT TNVG ++SVY+ KI  P+G TV V+P KL F    +K SFVV + 
Sbjct: 687 QYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVK 746

Query: 754 ANDQNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
             +  L+ G      G + WSDGK  V +PLVVT  +PL
Sbjct: 747 TTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQPL 775

BLAST of Carg05167 vs. TAIR10
Match: AT5G67360.1 (Subtilase family protein)

HSP 1 Score: 724.5 bits (1869), Expect = 6.7e-209
Identity = 390/770 (50.65%), Postives = 521/770 (67.66%), Query Frame = 0

Query: 9   LSSSIFFSLLLL--IHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTS---EFAESH 68
           LSS+ FF LL L   H S+   D    T+I  +     PS F  H +WY S     ++S 
Sbjct: 6   LSSTAFFLLLCLGFCHVSSSSSDQ--GTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA 65

Query: 69  KILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGL-RNQRGL 128
           ++L+ Y+   HGFS   TQE+ADS+   P V++V  + R +LHTTR+P FLGL  +   L
Sbjct: 66  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 125

Query: 129 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGA 188
           + ++   SDV++GV DTG+ PE +S+SD   GPIP  WKG C+ GT FTA  CNRK++GA
Sbjct: 126 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 185

Query: 189 RFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGI 248
           RFF++G+E   +  GP   I+++ E RSPRD DGHGTHT+STAAG     ASL GYASG 
Sbjct: 186 RFFARGYE---STMGP---IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGT 245

Query: 249 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDP 308
           A+G+AP+AR+AVYKVCW   GCF SDILAA D A+ D V+V+S+S+GGG    + YY D 
Sbjct: 246 ARGMAPRARVAVYKVCWL-GGCFSSDILAAIDKAIADNVNVLSMSLGGG---MSDYYRDG 305

Query: 309 IAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKI 368
           +AIGA+ A  RGI VS SAGN GP+  S++N+APWITTVGAGT+DR+FPA+ I+GN +  
Sbjct: 306 VAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNF 365

Query: 369 HGVSLYAGAPLNSTMYPLVYPGK-SGELSASLCMESSLDPKIVTGKIVICDRGSSPRVEK 428
            GVSL+ G  L   + P +Y G  S   + +LCM  +L P+ V GKIV+CDRG + RV+K
Sbjct: 366 TGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQK 425

Query: 429 GLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAF 488
           G VVK AGGVGMILAN  +NGE LV DAH+LPA  VG   GD ++ Y ++  NPTA+I+ 
Sbjct: 426 GDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISI 485

Query: 489 RGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKT 548
            GT +G+KP+PVVA+FS+RGPN + P ILKPD+IAPGVNILAAWT   GPTGL  D+R+ 
Sbjct: 486 LGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRV 545

Query: 549 EFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKA 608
           EFNI+SGTSM+CPH+SG AALLKS HP+WSPAA+RSA+MTTA  T    +P  + +TGK 
Sbjct: 546 EFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKP 605

Query: 609 STPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGTKMIQVISRTPVKC-PARKP 668
           STP+D GAGHV+   A +PGL+YD+T  DY+ FLC++ Y +  I+ +SR    C P++  
Sbjct: 606 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY 665

Query: 669 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEA-PKGATVKVKPSKL 728
              +LNYPS    F+    G+    + RT T+VG A + Y  K+ +   G  + V+P+ L
Sbjct: 666 SVADLNYPS----FAVNVDGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVL 725

Query: 729 VFSTAVKKQSFVVAISANDQNLTLGDVGAVFGSLSWSDGKHVVRTPLVVT 770
            F  A +K+S+ V  + +    +  +    FGS+ WSDGKHVV +P+ ++
Sbjct: 726 NFKEANEKKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAIS 755

BLAST of Carg05167 vs. TAIR10
Match: AT2G05920.1 (Subtilase family protein)

HSP 1 Score: 714.9 bits (1844), Expect = 5.3e-206
Identity = 372/736 (50.54%), Postives = 493/736 (66.98%), Query Frame = 0

Query: 34  TFIFRVDSFSKPSVFPTHFHWYTSEFAESHKILHVYDTVFHGFSATFTQEQADS-VGKHP 93
           T+I RV+   KP  F TH  WYTS+      +L+ Y T FHGFSA     +ADS +    
Sbjct: 29  TYIIRVNHSDKPESFLTHHDWYTSQLNSESSLLYTYTTSFHGFSAYLDSTEADSLLSSSN 88

Query: 94  SVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVN 153
           S+L +FED    LHTTR+P+FLGL ++ G+       + VIIGV DTG+ PE RSF D +
Sbjct: 89  SILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTD 148

Query: 154 LGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGHEAGANAAGPIIGINDTVEYRSPR 213
           +  IP +WKG C++G+ F +K CN+K++GAR FSKG +  +       G +   E  SPR
Sbjct: 149 MPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGG-----GFSSKRESVSPR 208

Query: 214 DADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAA 273
           D DGHGTHT++TAAG     AS  GYA+G A+G+A +AR+A YKVCW ++GCF SDILAA
Sbjct: 209 DVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCW-STGCFGSDILAA 268

Query: 274 FDSAVKDGVDVISISIGGGDGVSTPYYLDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVT 333
            D A+ DGVDV+S+S+GGG   S PYY D IAIGA+ A  RG+FVS SAGN GP   SV 
Sbjct: 269 MDRAILDGVDVLSLSLGGG---SAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVA 328

Query: 334 NLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYAGAPLNSTMYPLVYPGKSGELSAS 393
           N+APW+ TVGAGT+DR+FPA   +GN +++ GVSLY+G  + +    LVY  K    S++
Sbjct: 329 NVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY-NKGNSSSSN 388

Query: 394 LCMESSLDPKIVTGKIVICDRGSSPRVEKGLVVKKAGGVGMILANGISNGEGLVGDAHIL 453
           LC+  SLD  IV GKIV+CDRG + RVEKG VV+ AGG+GMI+AN  ++GE LV D+H+L
Sbjct: 389 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 448

Query: 454 PACAVGSVEGDAMKAYASSSGNPTATIAFRGTRIGIKPAPVVASFSARGPNGLNPEILKP 513
           PA AVG   GD ++ Y  S   PTA + F+GT + +KP+PVVA+FS+RGPN + PEILKP
Sbjct: 449 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKP 508

Query: 514 DMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNILSGTSMACPHISGAAALLKSAHPDWSP 573
           D+I PGVNILA W+D +GPTGLD D+R+T+FNI+SGTSM+CPHISG A LLK+AHP+WSP
Sbjct: 509 DVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSP 568

Query: 574 AAVRSAMMTTARITDNRRQPTTEESTGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYV 633
           +A++SA+MTTA + DN   P  + +    S PY  G+GHV+   A+ PGLVYDI+  +Y+
Sbjct: 569 SAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYI 628

Query: 634 NFLCSIGYGT-KMIQVISRTPVKCPARKPLPENLNYPSIVAVFSSLSKGLSTKSFIRTAT 693
            FLCS+ Y    ++ ++ R  V C  +   P  LNYPS    FS L  G     + R  T
Sbjct: 629 RFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPS----FSVLFGGKRVVRYTREVT 688

Query: 694 NVGAANSVYQAKIEAPKGATVKVKPSKLVF-STAVKKQSFVVAISANDQNLTLGDVGAVF 753
           NVGAA+SVY+  +       + VKPSKL F S   KK+  V  +S    ++T     A F
Sbjct: 689 NVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMT---NKAEF 747

Query: 754 GSLSWSDGKHVVRTPL 767
           GS++WS+ +H VR+P+
Sbjct: 749 GSITWSNPQHEVRSPV 747

BLAST of Carg05167 vs. TAIR10
Match: AT5G51750.1 (subtilase 1.3)

HSP 1 Score: 686.8 bits (1771), Expect = 1.5e-197
Identity = 375/779 (48.14%), Postives = 499/779 (64.06%), Query Frame = 0

Query: 18  LLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTS------------EFAESHKI 77
           L+ +   T       KT++  +D  + P  +  H  WY+S            E   +++I
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRI 79

Query: 78  LHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG--LW 137
           L+ Y T FHG +A  TQE+A+ + +   V+AV  + R +LHTTRSP FLGL  Q    +W
Sbjct: 80  LYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVW 139

Query: 138 SDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGAR 197
           ++     DV++GV DTGI PE  SF+D  + P+P  W+G C+TG +F  +NCNRKIVGAR
Sbjct: 140 AERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGAR 199

Query: 198 FFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIA 257
            F +G+EA          I++ +EY+SPRD DGHGTHTA+T AG     A+L G+A G A
Sbjct: 200 VFYRGYEAATGK------IDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTA 259

Query: 258 KGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDPI 317
           +G+A KAR+A YKVCW   GCF SDIL+A D AV DGV V+SIS+GG  GVST Y  D +
Sbjct: 260 RGMAQKARVAAYKVCWV-GGCFSSDILSAVDQAVADGVQVLSISLGG--GVST-YSRDSL 319

Query: 318 AIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIH 377
           +I  +GA   G+FVS SAGN GP+ +S+TN++PWITTVGA T+DR+FPA V +G  R   
Sbjct: 320 SIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFK 379

Query: 378 GVSLYAGAPL--NSTMYPLVYPGK--SGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 437
           GVSLY G  +   +  YPLVY G+  S     S C++ +LD + V GKIVICDRG +PRV
Sbjct: 380 GVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRV 439

Query: 438 EKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATI 497
           +KG VVK+AGG+GM+L N  +NGE LV D+H+LPA AVG  EG  +K YA +S   TA++
Sbjct: 440 QKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASL 499

Query: 498 AFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTR 557
              GTRIGIKP+PVVA+FS+RGPN L+ EILKPD++APGVNILAAWT  + P+ L  D R
Sbjct: 500 EILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPR 559

Query: 558 KTEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTG 617
           + +FNILSGTSM+CPH+SG AAL+KS HPDWSPAA++SA+MTTA + DN  +P T+ S  
Sbjct: 560 RVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGA 619

Query: 618 KASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGTKMIQVI----SRTPVKC 677
             S+PYD GAGH++   A DPGLVYDI   +Y  FLC+       ++V     +RT    
Sbjct: 620 APSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHT 679

Query: 678 PARKPLPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVK 737
            A+   P NLNYP+I A+F   +  +   +  RT TNVG   S Y+  +   KGA+V V+
Sbjct: 680 LAKN--PGNLNYPAISALFPE-NTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQ 739

Query: 738 PSKLVFSTAVKKQSFVVAISANDQNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           P  L F++  +K S+ V      +          FG L W    H VR+P+++T L PL
Sbjct: 740 PKTLNFTSKHQKLSYTVTFRTRFRMKR-----PEFGGLVWKSTTHKVRSPVIITWLPPL 780

BLAST of Carg05167 vs. Swiss-Prot
Match: sp|O49607|SBT16_ARATH (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 1142.1 bits (2953), Expect = 0.0e+00
Identity = 556/767 (72.49%), Postives = 658/767 (85.79%), Query Frame = 0

Query: 10  SSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFAESHKILHVY 69
           SS+I   L L     +       KTFIFR+D  S PS+FPTH+HWY++EFAE  +I+HVY
Sbjct: 3   SSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVY 62

Query: 70  DTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYG 129
            TVFHGFSA  T ++AD++  HP+VLAVFEDRRR+LHTTRSPQFLGL+NQ+GLWS+SDYG
Sbjct: 63  HTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYG 122

Query: 130 SDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGH 189
           SDVIIGVFDTGI PERRSFSD+NLGPIP+RW+GVC++G +F+ +NCNRKI+GARFF+KG 
Sbjct: 123 SDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQ 182

Query: 190 EAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPK 249
           +A       I GIN TVE+ SPRDADGHGTHT+STAAGRHAF+AS+ GYASG+AKGVAPK
Sbjct: 183 QAAV-----IGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPK 242

Query: 250 ARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDPIAIGAYG 309
           AR+A YKVCWK+SGC DSDILAAFD+AV+DGVDVISISIGGGDG+++PYYLDPIAIG+YG
Sbjct: 243 ARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYG 302

Query: 310 AASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYA 369
           AAS+GIFVSSSAGN+GPNGMSVTNLAPW+TTVGA TIDRNFPA  I+G+  ++ GVSLYA
Sbjct: 303 AASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYA 362

Query: 370 GAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVEKGLVVKKAG 429
           G PLN  M+P+VYPGKSG  SASLCME++LDPK V GKIVICDRGSSPRV KGLVVKKAG
Sbjct: 363 GVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAG 422

Query: 430 GVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAFRGTRIGIK 489
           GVGMILANG SNGEGLVGDAH++PACAVGS EGD +KAYASS  NP A+I FRGT +GIK
Sbjct: 423 GVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIK 482

Query: 490 PAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNILSGT 549
           PAPV+ASFS RGPNGL+PEILKPD+IAPGVNILAAWTD VGPTGL  D RKTEFNILSGT
Sbjct: 483 PAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGT 542

Query: 550 SMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKASTPYDFGA 609
           SMACPH+SGAAALLKSAHPDWSPA +RSAMMTT  + DN  +   +ESTGK++TPYD+G+
Sbjct: 543 SMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGS 602

Query: 610 GHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGTKMIQVISRTPVKCP-ARKPLPENLNYP 669
           GH+NLG AM+PGLVYDITN DY+ FLCSIGYG K IQVI+RTPV+CP  RKP P NLNYP
Sbjct: 603 GHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYP 662

Query: 670 SIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLVFSTAVKKQ 729
           SI AVF +  +GL +K+ IRTATNVG A +VY+A+IE+P+G TV VKP +LVF++AVK++
Sbjct: 663 SITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRR 722

Query: 730 SFVVAISANDQNLTLGDVGAVFGSLSWSD-GKHVVRTPLVVTKLEPL 775
           S+ V ++ N +N+ LG+ GAVFGS++W D GKHVVR+P+VVT+++ L
Sbjct: 723 SYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

BLAST of Carg05167 vs. Swiss-Prot
Match: sp|Q9LUM3|SBT15_ARATH (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 838.6 bits (2165), Expect = 5.7e-242
Identity = 437/759 (57.58%), Postives = 538/759 (70.88%), Query Frame = 0

Query: 34  TFIFRVDSFSKPSVFPTHFHWYTSEFA----ESHKILHVYDTVFHGFSATFTQEQADSVG 93
           T+I  VD  +KPS+FPTHFHWYTS  A        I+H YDTVFHGFSA  T + A  + 
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTYDTVFHGFSARLTSQDASQLL 86

Query: 94  KHPSVLAVFEDRRRQLHTTRSPQFLGLR--NQRGLWSDSDYGSDVIIGVFDTGISPERRS 153
            HP V++V  ++ R LHTTRSP+FLGLR  ++ GL  +SD+GSD++IGV DTG+ PER S
Sbjct: 87  DHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTGVWPERPS 146

Query: 154 FSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGHEAGANAAGPIIGINDTVE 213
           F D  LGP+P +WKG C     F    CNRK+VGARFF  G+EA          +N+T E
Sbjct: 147 FDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGK------MNETTE 206

Query: 214 YRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDS 273
           +RSPRD+DGHGTHTAS +AGR+ F AS  GYA G+A G+APKARLA YKVCW NSGC+DS
Sbjct: 207 FRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCW-NSGCYDS 266

Query: 274 DILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDPIAIGAYGAASRGIFVSSSAGNDGPN 333
           DILAAFD+AV DGVDVIS+S+G   GV  PYYLD IAIGA+GA  RGIFVS+SAGN GP 
Sbjct: 267 DILAAFDTAVADGVDVISLSVG---GVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPG 326

Query: 334 GMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYAGAPLN-STMYPLVYPGK- 393
            ++VTN+APW+TTVGAGTIDR+FPA V +GN + I GVS+Y G  L+   MYPLVY G  
Sbjct: 327 ALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSL 386

Query: 394 --SGELSASLCMESSLDPKIVTGKIVICDRGSSPRVEKGLVVKKAGGVGMILANGISNGE 453
                 S+SLC+E SLDP +V GKIV+CDRG + R  KG +V+K GG+GMI+ANG+ +GE
Sbjct: 387 LGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGE 446

Query: 454 GLVGDAHILPACAVGSVEGDAMKAYAS------SSGNPTATIAFRGTRIGIKPAPVVASF 513
           GLV D H+LPA +VG+  GD ++ Y S      SS +PTATI F+GTR+GI+PAPVVASF
Sbjct: 447 GLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASF 506

Query: 514 SARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNILSGTSMACPHIS 573
           SARGPN   PEILKPD+IAPG+NILAAW D +GP+G+  D R+TEFNILSGTSMACPH+S
Sbjct: 507 SARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVS 566

Query: 574 GAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKASTPYDFGAGHVNLGLA 633
           G AALLK+AHPDWSPAA+RSA++TTA   DN  +P  +ESTG  S+  D+G+GHV+   A
Sbjct: 567 GLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKA 626

Query: 634 MDPGLVYDITNTDYVNFLCSIGYGTKMIQVISRTPVKCPARKPLPE--NLNYPSIVAVFS 693
           MDPGLVYDIT+ DY+NFLC+  Y    I  I+R    C   +      NLNYPS   VF 
Sbjct: 627 MDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQ 686

Query: 694 SLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLVFSTAVKKQSFVVAIS 753
              +   +  FIRT TNVG ++SVY+ KI  P+G TV V+P KL F    +K SFVV + 
Sbjct: 687 QYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVK 746

Query: 754 ANDQNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
             +  L+ G      G + WSDGK  V +PLVVT  +PL
Sbjct: 747 TTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQPL 775

BLAST of Carg05167 vs. Swiss-Prot
Match: sp|O65351|SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 1.2e-207
Identity = 390/770 (50.65%), Postives = 521/770 (67.66%), Query Frame = 0

Query: 9   LSSSIFFSLLLL--IHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTS---EFAESH 68
           LSS+ FF LL L   H S+   D    T+I  +     PS F  H +WY S     ++S 
Sbjct: 6   LSSTAFFLLLCLGFCHVSSSSSDQ--GTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA 65

Query: 69  KILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGL-RNQRGL 128
           ++L+ Y+   HGFS   TQE+ADS+   P V++V  + R +LHTTR+P FLGL  +   L
Sbjct: 66  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 125

Query: 129 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGA 188
           + ++   SDV++GV DTG+ PE +S+SD   GPIP  WKG C+ GT FTA  CNRK++GA
Sbjct: 126 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 185

Query: 189 RFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGI 248
           RFF++G+E   +  GP   I+++ E RSPRD DGHGTHT+STAAG     ASL GYASG 
Sbjct: 186 RFFARGYE---STMGP---IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGT 245

Query: 249 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDP 308
           A+G+AP+AR+AVYKVCW   GCF SDILAA D A+ D V+V+S+S+GGG    + YY D 
Sbjct: 246 ARGMAPRARVAVYKVCWL-GGCFSSDILAAIDKAIADNVNVLSMSLGGG---MSDYYRDG 305

Query: 309 IAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKI 368
           +AIGA+ A  RGI VS SAGN GP+  S++N+APWITTVGAGT+DR+FPA+ I+GN +  
Sbjct: 306 VAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNF 365

Query: 369 HGVSLYAGAPLNSTMYPLVYPGK-SGELSASLCMESSLDPKIVTGKIVICDRGSSPRVEK 428
            GVSL+ G  L   + P +Y G  S   + +LCM  +L P+ V GKIV+CDRG + RV+K
Sbjct: 366 TGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQK 425

Query: 429 GLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAF 488
           G VVK AGGVGMILAN  +NGE LV DAH+LPA  VG   GD ++ Y ++  NPTA+I+ 
Sbjct: 426 GDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISI 485

Query: 489 RGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKT 548
            GT +G+KP+PVVA+FS+RGPN + P ILKPD+IAPGVNILAAWT   GPTGL  D+R+ 
Sbjct: 486 LGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRV 545

Query: 549 EFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKA 608
           EFNI+SGTSM+CPH+SG AALLKS HP+WSPAA+RSA+MTTA  T    +P  + +TGK 
Sbjct: 546 EFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKP 605

Query: 609 STPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGTKMIQVISRTPVKC-PARKP 668
           STP+D GAGHV+   A +PGL+YD+T  DY+ FLC++ Y +  I+ +SR    C P++  
Sbjct: 606 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY 665

Query: 669 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEA-PKGATVKVKPSKL 728
              +LNYPS    F+    G+    + RT T+VG A + Y  K+ +   G  + V+P+ L
Sbjct: 666 SVADLNYPS----FAVNVDGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVL 725

Query: 729 VFSTAVKKQSFVVAISANDQNLTLGDVGAVFGSLSWSDGKHVVRTPLVVT 770
            F  A +K+S+ V  + +    +  +    FGS+ WSDGKHVV +P+ ++
Sbjct: 726 NFKEANEKKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAIS 755

BLAST of Carg05167 vs. Swiss-Prot
Match: sp|Q9ZUF6|SBT18_ARATH (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 9.5e-205
Identity = 372/736 (50.54%), Postives = 493/736 (66.98%), Query Frame = 0

Query: 34  TFIFRVDSFSKPSVFPTHFHWYTSEFAESHKILHVYDTVFHGFSATFTQEQADS-VGKHP 93
           T+I RV+   KP  F TH  WYTS+      +L+ Y T FHGFSA     +ADS +    
Sbjct: 29  TYIIRVNHSDKPESFLTHHDWYTSQLNSESSLLYTYTTSFHGFSAYLDSTEADSLLSSSN 88

Query: 94  SVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVN 153
           S+L +FED    LHTTR+P+FLGL ++ G+       + VIIGV DTG+ PE RSF D +
Sbjct: 89  SILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTD 148

Query: 154 LGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGHEAGANAAGPIIGINDTVEYRSPR 213
           +  IP +WKG C++G+ F +K CN+K++GAR FSKG +  +       G +   E  SPR
Sbjct: 149 MPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGG-----GFSSKRESVSPR 208

Query: 214 DADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAA 273
           D DGHGTHT++TAAG     AS  GYA+G A+G+A +AR+A YKVCW ++GCF SDILAA
Sbjct: 209 DVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCW-STGCFGSDILAA 268

Query: 274 FDSAVKDGVDVISISIGGGDGVSTPYYLDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVT 333
            D A+ DGVDV+S+S+GGG   S PYY D IAIGA+ A  RG+FVS SAGN GP   SV 
Sbjct: 269 MDRAILDGVDVLSLSLGGG---SAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVA 328

Query: 334 NLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYAGAPLNSTMYPLVYPGKSGELSAS 393
           N+APW+ TVGAGT+DR+FPA   +GN +++ GVSLY+G  + +    LVY  K    S++
Sbjct: 329 NVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY-NKGNSSSSN 388

Query: 394 LCMESSLDPKIVTGKIVICDRGSSPRVEKGLVVKKAGGVGMILANGISNGEGLVGDAHIL 453
           LC+  SLD  IV GKIV+CDRG + RVEKG VV+ AGG+GMI+AN  ++GE LV D+H+L
Sbjct: 389 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 448

Query: 454 PACAVGSVEGDAMKAYASSSGNPTATIAFRGTRIGIKPAPVVASFSARGPNGLNPEILKP 513
           PA AVG   GD ++ Y  S   PTA + F+GT + +KP+PVVA+FS+RGPN + PEILKP
Sbjct: 449 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKP 508

Query: 514 DMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNILSGTSMACPHISGAAALLKSAHPDWSP 573
           D+I PGVNILA W+D +GPTGLD D+R+T+FNI+SGTSM+CPHISG A LLK+AHP+WSP
Sbjct: 509 DVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSP 568

Query: 574 AAVRSAMMTTARITDNRRQPTTEESTGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYV 633
           +A++SA+MTTA + DN   P  + +    S PY  G+GHV+   A+ PGLVYDI+  +Y+
Sbjct: 569 SAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYI 628

Query: 634 NFLCSIGYGT-KMIQVISRTPVKCPARKPLPENLNYPSIVAVFSSLSKGLSTKSFIRTAT 693
            FLCS+ Y    ++ ++ R  V C  +   P  LNYPS    FS L  G     + R  T
Sbjct: 629 RFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPS----FSVLFGGKRVVRYTREVT 688

Query: 694 NVGAANSVYQAKIEAPKGATVKVKPSKLVF-STAVKKQSFVVAISANDQNLTLGDVGAVF 753
           NVGAA+SVY+  +       + VKPSKL F S   KK+  V  +S    ++T     A F
Sbjct: 689 NVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMT---NKAEF 747

Query: 754 GSLSWSDGKHVVRTPL 767
           GS++WS+ +H VR+P+
Sbjct: 749 GSITWSNPQHEVRSPV 747

BLAST of Carg05167 vs. Swiss-Prot
Match: sp|Q9FLI4|SBT13_ARATH (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 2.8e-196
Identity = 375/779 (48.14%), Postives = 499/779 (64.06%), Query Frame = 0

Query: 18  LLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTS------------EFAESHKI 77
           L+ +   T       KT++  +D  + P  +  H  WY+S            E   +++I
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRI 79

Query: 78  LHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG--LW 137
           L+ Y T FHG +A  TQE+A+ + +   V+AV  + R +LHTTRSP FLGL  Q    +W
Sbjct: 80  LYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVW 139

Query: 138 SDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGAR 197
           ++     DV++GV DTGI PE  SF+D  + P+P  W+G C+TG +F  +NCNRKIVGAR
Sbjct: 140 AERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGAR 199

Query: 198 FFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIA 257
            F +G+EA          I++ +EY+SPRD DGHGTHTA+T AG     A+L G+A G A
Sbjct: 200 VFYRGYEAATGK------IDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTA 259

Query: 258 KGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDPI 317
           +G+A KAR+A YKVCW   GCF SDIL+A D AV DGV V+SIS+GG  GVST Y  D +
Sbjct: 260 RGMAQKARVAAYKVCWV-GGCFSSDILSAVDQAVADGVQVLSISLGG--GVST-YSRDSL 319

Query: 318 AIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIH 377
           +I  +GA   G+FVS SAGN GP+ +S+TN++PWITTVGA T+DR+FPA V +G  R   
Sbjct: 320 SIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFK 379

Query: 378 GVSLYAGAPL--NSTMYPLVYPGK--SGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 437
           GVSLY G  +   +  YPLVY G+  S     S C++ +LD + V GKIVICDRG +PRV
Sbjct: 380 GVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRV 439

Query: 438 EKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATI 497
           +KG VVK+AGG+GM+L N  +NGE LV D+H+LPA AVG  EG  +K YA +S   TA++
Sbjct: 440 QKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASL 499

Query: 498 AFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTR 557
              GTRIGIKP+PVVA+FS+RGPN L+ EILKPD++APGVNILAAWT  + P+ L  D R
Sbjct: 500 EILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPR 559

Query: 558 KTEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTG 617
           + +FNILSGTSM+CPH+SG AAL+KS HPDWSPAA++SA+MTTA + DN  +P T+ S  
Sbjct: 560 RVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGA 619

Query: 618 KASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGTKMIQVI----SRTPVKC 677
             S+PYD GAGH++   A DPGLVYDI   +Y  FLC+       ++V     +RT    
Sbjct: 620 APSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHT 679

Query: 678 PARKPLPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVK 737
            A+   P NLNYP+I A+F   +  +   +  RT TNVG   S Y+  +   KGA+V V+
Sbjct: 680 LAKN--PGNLNYPAISALFPE-NTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQ 739

Query: 738 PSKLVFSTAVKKQSFVVAISANDQNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           P  L F++  +K S+ V      +          FG L W    H VR+P+++T L PL
Sbjct: 740 PKTLNFTSKHQKLSYTVTFRTRFRMKR-----PEFGGLVWKSTTHKVRSPVIITWLPPL 780

BLAST of Carg05167 vs. TrEMBL
Match: tr|A0A0A0LJ58|A0A0A0LJ58_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G270180 PE=4 SV=1)

HSP 1 Score: 1385.5 bits (3585), Expect = 0.0e+00
Identity = 693/774 (89.53%), Postives = 731/774 (94.44%), Query Frame = 0

Query: 1   MAACFAASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60
           MAA  + SL SS FFS LLL+  STV    PLKTFI R+D FSKPSVFPTH+HWYTSEF 
Sbjct: 1   MAAFLSTSL-SSFFFSFLLLL--STVSSHSPLKTFIVRIDRFSKPSVFPTHYHWYTSEFT 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           +S +ILHVYDTVFHGFSAT TQ+Q DS+GKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  QSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAGANAAGPIIGINDT+EYRSPRDADGHGTHTASTAAGRH+FQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFD+AV DGVDVISISIGGGDGVS+PYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIG+YGAAS+G+FVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFP+VV +GN R
Sbjct: 301 DPIAIGSYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGNGR 360

Query: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420
           KI+GVSLYAGAPLN TMYPLVYPGKSG LS SLCME+SLDPK+VTGKIVICDRGSSPRV 
Sbjct: 361 KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAH+LPACAVGS EGDAMKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           F+GT IGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTD VGPTGLDFD RK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDKRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
           TEFNILSGTSMACPH+SGAAALLKSAHPDWSPAA+RSAMMTTA ITDNRRQP TEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGTKMIQVISRTPVKCPARKP 660
            STPYDFGAGHVNLGLAMDPGL+YDITNTDY+NFLCSIGYG KMIQVI+RTPV+CP +KP
Sbjct: 601 PSTPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV 720
           LPENLNYPSIV VFSSLSKG STKSFIRTATNVG +NSVY+ KIEAPKG TVKVKPSKLV
Sbjct: 661 LPENLNYPSIVTVFSSLSKGWSTKSFIRTATNVGPSNSVYRVKIEAPKGVTVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANDQNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           FST VKKQSFVVAISA++QNL LGDVGAVFG LSWSDGKHVVR+PLVVT+LEPL
Sbjct: 721 FSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of Carg05167 vs. TrEMBL
Match: tr|A0A1S3C7D0|A0A1S3C7D0_CUCME (subtilisin-like protease SBT1.6 OS=Cucumis melo OX=3656 GN=LOC103497323 PE=4 SV=1)

HSP 1 Score: 1382.5 bits (3577), Expect = 0.0e+00
Identity = 693/774 (89.53%), Postives = 729/774 (94.19%), Query Frame = 0

Query: 1   MAACFAASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60
           MAA  + SL S  FFS LLL+  STV    PLKTFI R+D FSKPSVFPTH+HWYTSEF 
Sbjct: 1   MAAFLSTSL-SPFFFSFLLLL--STVSSLSPLKTFIVRIDRFSKPSVFPTHYHWYTSEFT 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           +S +ILHVYDTVFHGFSAT TQEQ DS+GKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  QSPQILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAGANAAGPIIGINDT+EYRSPRDADGHGTHTASTAAGRH+FQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFD+AV DGVDVISISIGGGDGVS+PYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAAS+G+FVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFP+VV +GN R
Sbjct: 301 DPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGNGR 360

Query: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420
           KI+GVSLYAGAPLN TMYPLVYPGKSG LS SLCME+SLDPK+VTGKIVICDRGSSPRV 
Sbjct: 361 KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAH+LPACAVGS EGDAMKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           F+GT IGIKPAPVVASFSARGPNGL PEILKPD+IAPGVNILAAWTD VGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLTPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
           TEFNILSGTSMACPH+SGAAALLKSAHPDWSPAA+RSAMMTTA I DNRRQP TEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASIIDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGTKMIQVISRTPVKCPARKP 660
            STPYDFGAGHVNLGLAMDPGL+YDITNTDY+NFLCSIGYG KMIQVI+RTPV+CP +KP
Sbjct: 601 PSTPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV 720
           LPENLNYPSIVAVFSSLSKG STKSFIRT TNVG +NSVY+ KIEAPKG TVKVKPSKLV
Sbjct: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPSNSVYRVKIEAPKGVTVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANDQNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           FST VKKQSFVVAISA++QNL LGDVGAVFG LSWSDGKHVVR+PLVVT+LEPL
Sbjct: 721 FSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of Carg05167 vs. TrEMBL
Match: tr|A0A2P5EWU1|A0A2P5EWU1_9ROSA (Subtilase OS=Trema orientalis OX=63057 GN=TorSBT6 PE=4 SV=1)

HSP 1 Score: 1226.1 bits (3171), Expect = 0.0e+00
Identity = 590/742 (79.51%), Postives = 665/742 (89.62%), Query Frame = 0

Query: 33  KTFIFRVDSFSKPSVFPTHFHWYTSEFAESHKILHVYDTVFHGFSATFTQEQADSVGKHP 92
           KTFIFRVDS SKPS+FPTH+HWYT+EFA+  +ILH YDTVFHGFSA  + +QA SV +HP
Sbjct: 32  KTFIFRVDSHSKPSIFPTHYHWYTTEFADPPQILHTYDTVFHGFSAVLSPDQAASVSRHP 91

Query: 93  SVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVN 152
           SVLAVFED+RRQLHTTRSPQFLGLRNQ+GLWS+SDYGSDVI+GVFDTGI PERRSFSD+N
Sbjct: 92  SVLAVFEDQRRQLHTTRSPQFLGLRNQKGLWSESDYGSDVIVGVFDTGIWPERRSFSDLN 151

Query: 153 LGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGHEAGANAAGPIIGINDTVEYRSPR 212
           LGPIP RWKG C+ G +F+AKNCNRKI+GARFF+KGHEA  N  GP+ G+NDTVE+RSPR
Sbjct: 152 LGPIPSRWKGACEVGVRFSAKNCNRKIIGARFFAKGHEAAGNVGGPVSGVNDTVEFRSPR 211

Query: 213 DADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAA 272
           DADGHGTHTASTAAGR+AF AS+ GYASGIAKGVAPKARLA YKVCWKNSGCFDSDILAA
Sbjct: 212 DADGHGTHTASTAAGRYAFDASMAGYASGIAKGVAPKARLAAYKVCWKNSGCFDSDILAA 271

Query: 273 FDSAVKDGVDVISISIGGGDGVSTPYYLDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVT 332
           FD+AV DGVDVISISIGGGDG+S+PYYLDPIAIGAYGA S+G+FVSSSAGNDGPNGMSVT
Sbjct: 272 FDAAVNDGVDVISISIGGGDGISSPYYLDPIAIGAYGAVSKGVFVSSSAGNDGPNGMSVT 331

Query: 333 NLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYAGAPLNSTMYPLVYPGKSGELSAS 392
           N+APW+T+VGAGTIDRNFPAV+++G+ R++ GVSLYAGAPL   MYP+VYPGKSG L AS
Sbjct: 332 NVAPWMTSVGAGTIDRNFPAVIVLGDGRRLSGVSLYAGAPLKGKMYPVVYPGKSGMLPAS 391

Query: 393 LCMESSLDPKIVTGKIVICDRGSSPRVEKGLVVKKAGGVGMILANGISNGEGLVGDAHIL 452
           LCME+SLDP +V GKIVICDRGSSPRV KG+VVK AGGVGMILANG+S+GEGLVGDAH++
Sbjct: 392 LCMENSLDPNLVRGKIVICDRGSSPRVAKGVVVKNAGGVGMILANGVSHGEGLVGDAHLI 451

Query: 453 PACAVGSVEGDAMKAYASSSGNPTATIAFRGTRIGIKPAPVVASFSARGPNGLNPEILKP 512
           PACAVGS EG+A+KAY SS  NPTATI F GT IGIKPAP+VASFS RGPN LNPEILKP
Sbjct: 452 PACAVGSDEGNAVKAYVSSGKNPTATIDFHGTVIGIKPAPIVASFSGRGPNTLNPEILKP 511

Query: 513 DMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNILSGTSMACPHISGAAALLKSAHPDWSP 572
           D+IAPGVNILAAWTD VGPTGLD DTRKTEFNILSGTSMACPH+SGAAALLKSAHPDWSP
Sbjct: 512 DLIAPGVNILAAWTDAVGPTGLDSDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSP 571

Query: 573 AAVRSAMMTTARITDNRRQPTTEESTGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYV 632
           AA+RSAMMTTA   DNR QP T+E+TGK STPYDFGAGH+NL  AMDPGLVYDITN DYV
Sbjct: 572 AAIRSAMMTTASTVDNRNQPMTDEATGKPSTPYDFGAGHLNLDRAMDPGLVYDITNNDYV 631

Query: 633 NFLCSIGYGTKMIQVISRTPVKCPARKPLPENLNYPSIVAVFSSLSKGLSTKSFIRTATN 692
           NFLCSIGYG K+IQVI+RTPV+CPAR+P  ENLNYPS  A+FS+ +KG  +K FIRT TN
Sbjct: 632 NFLCSIGYGPKVIQVITRTPVRCPARRPASENLNYPSFSALFSTSTKGKESKMFIRTVTN 691

Query: 693 VGAANSVYQAKIEAPKGATVKVKPSKLVFSTAVKKQSFVVAISANDQNLTLGDVGAVFGS 752
           VGA NSVY+  +EAPKG TV VKP+KLVFS AVKK+SFVV +SA+ +NL +GD GA FGS
Sbjct: 692 VGAPNSVYRVSVEAPKGVTVAVKPAKLVFSEAVKKRSFVVTVSADSRNLVVGDSGAAFGS 751

Query: 753 LSWSDGKHVVRTPLVVTKLEPL 775
           LSW+DGKHVVR+P+VVT++ PL
Sbjct: 752 LSWTDGKHVVRSPIVVTQIVPL 773

BLAST of Carg05167 vs. TrEMBL
Match: tr|W9QT04|W9QT04_9ROSA (Subtilisin-like protease OS=Morus notabilis OX=981085 GN=L484_024992 PE=4 SV=1)

HSP 1 Score: 1221.5 bits (3159), Expect = 0.0e+00
Identity = 590/743 (79.41%), Postives = 672/743 (90.44%), Query Frame = 0

Query: 32  LKTFIFRVDSFSKPSVFPTHFHWYTSEFAESHKILHVYDTVFHGFSATFTQEQADSVGKH 91
           LKTFIFRVDS +KPS+FPTH+HWYT+EFA+  +ILHVYDTVF+GFSA  + +Q     +H
Sbjct: 36  LKTFIFRVDSHTKPSIFPTHYHWYTTEFADPPQILHVYDTVFNGFSAVLSSDQVAYASRH 95

Query: 92  PSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDV 151
           PSVLAVFED+RRQLHTTRSPQFLGLRNQRGLWS+SDYGSDVIIGVFDTGI PERRSFSD+
Sbjct: 96  PSVLAVFEDKRRQLHTTRSPQFLGLRNQRGLWSESDYGSDVIIGVFDTGIWPERRSFSDL 155

Query: 152 NLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGHEAGANAAGPIIGINDTVEYRSP 211
           NLGPIP RWKGVC++G KF+ +NCNRK++GARFFSKGHEA  +  GPI G+NDT+E+RSP
Sbjct: 156 NLGPIPSRWKGVCESGAKFSVRNCNRKLIGARFFSKGHEAAGSIGGPISGVNDTLEFRSP 215

Query: 212 RDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILA 271
           RDADGHGTHTASTAAGR+AF+AS+ GYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILA
Sbjct: 216 RDADGHGTHTASTAAGRYAFEASMAGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILA 275

Query: 272 AFDSAVKDGVDVISISIGGGDGVSTPYYLDPIAIGAYGAASRGIFVSSSAGNDGPNGMSV 331
           AFD+AV DGVDVISISIGGGDG+S+PYYLDPIAIGAYGA S+G+FVSSSAGNDGPNGMSV
Sbjct: 276 AFDAAVNDGVDVISISIGGGDGISSPYYLDPIAIGAYGAVSKGVFVSSSAGNDGPNGMSV 335

Query: 332 TNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYAGAPLNSTMYPLVYPGKSGELSA 391
           TNLAPW+TTVGAGTIDR FPAV+++G+ R++ GVSLYAGAPL   MYPLVYPGKSG L A
Sbjct: 336 TNLAPWMTTVGAGTIDRTFPAVIVLGDGRRLSGVSLYAGAPLKGKMYPLVYPGKSGMLPA 395

Query: 392 SLCMESSLDPKIVTGKIVICDRGSSPRVEKGLVVKKAGGVGMILANGISNGEGLVGDAHI 451
           SLCME+SLDP +V GKIVICDRGSSPRV KG+VVKKAGGVGMIL+NGIS G GLVGDAHI
Sbjct: 396 SLCMENSLDPNLVRGKIVICDRGSSPRVAKGMVVKKAGGVGMILSNGISQGGGLVGDAHI 455

Query: 452 LPACAVGSVEGDAMKAYASSSGNPTATIAFRGTRIGIKPAPVVASFSARGPNGLNPEILK 511
           LPACAVGS EG+A+KAY SS+ NPTATI F+GT IGIKPAP+VASFS RGPN +NPEILK
Sbjct: 456 LPACAVGSDEGNAVKAYVSSASNPTATIDFQGTVIGIKPAPIVASFSGRGPNSVNPEILK 515

Query: 512 PDMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNILSGTSMACPHISGAAALLKSAHPDWS 571
           PD+IAPGVNILAAWTD VGPTGLD D RKTEFNILSGTSMACPH+SGAAALLKSAHPDWS
Sbjct: 516 PDLIAPGVNILAAWTDAVGPTGLDSDKRKTEFNILSGTSMACPHVSGAAALLKSAHPDWS 575

Query: 572 PAAVRSAMMTTARITDNRRQPTTEESTGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDY 631
           PAA+RSAMMTTA I DNR Q  T+ESTGK+STPYD GAGH+NL  AMDPGLVYDITN D+
Sbjct: 576 PAAIRSAMMTTASIVDNRNQLMTDESTGKSSTPYDLGAGHLNLDRAMDPGLVYDITNDDH 635

Query: 632 VNFLCSIGYGTKMIQVISRTPVKCPARKPLPENLNYPSIVAVFSSLSKGLSTKSFIRTAT 691
           VNFLCSIGYG K+IQVI+RTPVKCP ++PLPENLNYPS+ A+F + S+G ++K FIRT T
Sbjct: 636 VNFLCSIGYGPKVIQVITRTPVKCPVKRPLPENLNYPSMAALFPTSSRGSTSKMFIRTVT 695

Query: 692 NVGAANSVYQAKIEAPKGATVKVKPSKLVFSTAVKKQSFVVAISANDQNLTLGDVGAVFG 751
           NVGA NSVY+A+IEAPKG TV+VKP+KLVF+ AVKKQSFVV ++A+ ++L LG+ GA FG
Sbjct: 696 NVGAPNSVYRARIEAPKGVTVRVKPAKLVFTEAVKKQSFVVTVTADARSLVLGESGANFG 755

Query: 752 SLSWSDGKHVVRTPLVVTKLEPL 775
           SLSW+DGKHVVR+P+VVT+++PL
Sbjct: 756 SLSWTDGKHVVRSPIVVTEIQPL 778

BLAST of Carg05167 vs. TrEMBL
Match: tr|V4SFS4|V4SFS4_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10027859mg PE=4 SV=1)

HSP 1 Score: 1220.3 bits (3156), Expect = 0.0e+00
Identity = 605/779 (77.66%), Postives = 683/779 (87.68%), Query Frame = 0

Query: 1   MAACFAASLSSSIFFSLLL---LIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTS 60
           MAA   +SL     F LLL    +   T+  D  +KTFIFR+DS SKPS+FPTH+HWY+S
Sbjct: 1   MAAIPYSSLPLQFLFFLLLSGSFLQTRTLSTDQTVKTFIFRIDSQSKPSIFPTHYHWYSS 60

Query: 61  EFAESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLR 120
           EFA   +ILH YDTVFHGFSAT + +QA S+ +HPSVLAV ED+RRQLHTTRSPQFLGLR
Sbjct: 61  EFASPVQILHTYDTVFHGFSATLSPDQAASLSRHPSVLAVIEDQRRQLHTTRSPQFLGLR 120

Query: 121 NQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNR 180
           NQ+GLWS+SDYGSDVIIGVFDTGI PERRSFSD+N+G IP +WKGVCQ G KFTAKNCN+
Sbjct: 121 NQQGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNIGSIPSKWKGVCQVGVKFTAKNCNK 180

Query: 181 KIVGARFFSKGHEAGANAAGPI-IGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLE 240
           KI+GARFFSKGHEA   +AGPI  GIN+TVE+ SPRDADGHGTHTASTAAGRHAF+AS+E
Sbjct: 181 KIIGARFFSKGHEAAGGSAGPIGGGINETVEFMSPRDADGHGTHTASTAAGRHAFRASME 240

Query: 241 GYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVST 300
           GYA+G+AKGVAPKARLAVYKVCWKN+GCFDSDILAAFD+AV DGVDVISISIGGGDG+S+
Sbjct: 241 GYAAGVAKGVAPKARLAVYKVCWKNAGCFDSDILAAFDAAVNDGVDVISISIGGGDGISS 300

Query: 301 PYYLDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIM 360
           PYYLDPIAIG+YGAASRG+FVSSSAGNDGPNGMSVTNLAPWI TVGAGTIDRNFPA V +
Sbjct: 301 PYYLDPIAIGSYGAASRGVFVSSSAGNDGPNGMSVTNLAPWIVTVGAGTIDRNFPAEVRL 360

Query: 361 GNRRKIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSS 420
           G+ R++ GVSLYAGAPL+  MYPL+YPGKSG LSASLCME+SLDP +V GKIVICDRGSS
Sbjct: 361 GDGRRLSGVSLYAGAPLSEKMYPLIYPGKSGVLSASLCMENSLDPNLVRGKIVICDRGSS 420

Query: 421 PRVEKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPT 480
           PRV KGLVVKKAGGVGMILANGISNGEGLVGDAH+LPACA+GS EGDA+KAY SS+ NPT
Sbjct: 421 PRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACALGSDEGDAVKAYISSTANPT 480

Query: 481 ATIAFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDF 540
           ATI F+GT +GIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWT+ VGPTGLD 
Sbjct: 481 ATIDFKGTILGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTEAVGPTGLDS 540

Query: 541 DTRKTEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEE 600
           D RKTEFNILSGTSMACPH+SGAAALLKSAHPDWSPAA+RSAMMTTA I DN  QP T+E
Sbjct: 541 DLRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAAIRSAMMTTASIVDNSNQPMTDE 600

Query: 601 STGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGTKMIQVISRTPVKCP 660
           +TG ASTPYDFGAGHVNL  AMDPGLVYDITN DYVNFLC+ GYG K+IQVI+R P +CP
Sbjct: 601 ATGNASTPYDFGAGHVNLDRAMDPGLVYDITNDDYVNFLCANGYGPKLIQVITRIPARCP 660

Query: 661 ARKPLPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAP-KGATVKVK 720
           A++P PENLNYPSI A+FS+ S+G+S+KSFIRT TNVG  N+VY  K+ +P KG TV VK
Sbjct: 661 AKRPRPENLNYPSIAALFSTQSRGVSSKSFIRTVTNVGQPNAVYTVKVVSPEKGVTVTVK 720

Query: 721 PSKLVFSTAVKKQSFVVAISANDQNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           PS+LVF+  VKK SFVV ++A+ +NL L D GA FGS+SWSDGKH VR+PLVVT+L+PL
Sbjct: 721 PSRLVFTEGVKKSSFVVTVTADSKNLVLNDSGAAFGSISWSDGKHEVRSPLVVTQLDPL 779

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022946948.10.0e+0099.22subtilisin-like protease SBT1.6 [Cucurbita moschata][more]
XP_023532451.10.0e+0098.45subtilisin-like protease SBT1.6 [Cucurbita pepo subsp. pepo][more]
XP_022970938.10.0e+0097.03subtilisin-like protease SBT1.6 [Cucurbita maxima][more]
XP_023512905.10.0e+0089.61subtilisin-like protease SBT1.6 [Cucurbita pepo subsp. pepo][more]
XP_022985703.10.0e+0089.86subtilisin-like protease SBT1.6 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G34980.10.0e+0072.49subtilisin-like serine protease 2[more]
AT3G14240.13.2e-24357.58Subtilase family protein[more]
AT5G67360.16.7e-20950.65Subtilase family protein[more]
AT2G05920.15.3e-20650.54Subtilase family protein[more]
AT5G51750.11.5e-19748.14subtilase 1.3[more]
Match NameE-valueIdentityDescription
sp|O49607|SBT16_ARATH0.0e+0072.49Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S... [more]
sp|Q9LUM3|SBT15_ARATH5.7e-24257.58Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... [more]
sp|O65351|SBT17_ARATH1.2e-20750.65Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
sp|Q9ZUF6|SBT18_ARATH9.5e-20550.54Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... [more]
sp|Q9FLI4|SBT13_ARATH2.8e-19648.14Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 S... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0LJ58|A0A0A0LJ58_CUCSA0.0e+0089.53Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G270180 PE=4 SV=1[more]
tr|A0A1S3C7D0|A0A1S3C7D0_CUCME0.0e+0089.53subtilisin-like protease SBT1.6 OS=Cucumis melo OX=3656 GN=LOC103497323 PE=4 SV=... [more]
tr|A0A2P5EWU1|A0A2P5EWU1_9ROSA0.0e+0079.51Subtilase OS=Trema orientalis OX=63057 GN=TorSBT6 PE=4 SV=1[more]
tr|W9QT04|W9QT04_9ROSA0.0e+0079.41Subtilisin-like protease OS=Morus notabilis OX=981085 GN=L484_024992 PE=4 SV=1[more]
tr|V4SFS4|V4SFS4_9ROSI0.0e+0077.66Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10027859mg PE=4 ... [more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: INTERPRO
TermDefinition
IPR034197Peptidases_S8_3
IPR023828Peptidase_S8_Ser-AS
IPR037045S8pro/Inhibitor_I9_sf
IPR010259S8pro/Inhibitor_I9
IPR000209Peptidase_S8/S53_dom
IPR003137PA_domain
IPR036852Peptidase_S8/S53_dom_sf
IPR015500Peptidase_S8_subtilisin-rel
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05167-RACarg05167-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 213..226
score: 50.0
coord: 547..563
score: 58.55
coord: 129..148
score: 32.4
IPR036852Peptidase S8/S53 domain superfamilyGENE3DG3DSA:3.40.50.200coord: 131..346
e-value: 6.9E-178
score: 594.2
coord: 488..632
e-value: 6.9E-178
score: 594.2
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILYSSF52743Subtilisin-likecoord: 494..622
coord: 105..385
IPR003137PA domainPFAMPF02225PAcoord: 378..464
e-value: 8.0E-9
score: 35.4
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 129..610
e-value: 2.9E-47
score: 161.3
NoneNo IPR availableGENE3DG3DSA:2.60.40.2310coord: 635..771
e-value: 3.4E-37
score: 129.1
NoneNo IPR availableGENE3DG3DSA:3.50.30.30coord: 347..487
e-value: 6.9E-178
score: 594.2
NoneNo IPR availablePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 14..772
NoneNo IPR availablePANTHERPTHR10795:SF442SUBTILISIN-LIKE PROTEASE SBT1.6coord: 14..772
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 353..479
e-value: 2.40675E-37
score: 135.619
NoneNo IPR availableSUPERFAMILYSSF52025PA domaincoord: 404..473
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 48..106
e-value: 1.4E-10
score: 41.6
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3DG3DSA:3.30.70.80coord: 46..106
e-value: 1.0E-8
score: 37.5
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 548..558
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 104..583
e-value: 1.42997E-142
score: 423.548

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg05167Carg16178Silver-seed gourdcarcarB373