CmaCh01G008300 (gene) Cucurbita maxima (Rimu)

NameCmaCh01G008300
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptiontRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
LocationCma_Chr01 : 4441241 .. 4454165 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAGAAGGAGCAGCAGCAGAAGCAGCGAAGCACCGCGGCTGCCCTTCTCGTCTCTTCAGCGGAGGAAACTGTAAGCCATGGAAGACATAGAAGACCTGCTCATCGGCGGCGGCGGCGGCGGTGGAGCTCCCCCTGGTTACCGACTTCCAATATCCGCAGTCGGTGTAAAGTCGAAGAAAAAGAATAAGCTGAAACCTAATCCTGATGCTGATGATTCCAGCAGCGGGCTTTTTCCAACCCAAAATCTACTGGCACCTAAGATTCCAGGCACTCAGGTCTCTCTCTCTCTTCATATCTTCTTTTAACGCCTTGAGTGTTTTTTTGTTTTATGTTCACATTTCTCTTGCGGTACTGATAACTTTATGCGTCGATGTATTTTGGCAGACAATTTATGTTAAAACCTTTGGATGTTCTCACAACCAGGTTAGTCCTTCACACCTCGCGTATTAAATTGATTTCTTTTGTTTCCTTGGCTTAAATGAATGGCTGAGTCTTTAATCCCGTTAGAGTTTGTTTATTGTATACTTGTCCAAATAGAGGTTGATTGGTTATTAGTTTTTATCCTTGCAATTTGTTGTGACATGAAACTGGATTTTACTATTTCCACTGTGCATTCCACAATTGTTTCATCTTTTATGGCGCACAATTGTTTCATCTTTTATGGCGTTTTATCTAAGGCTTCCAGTTGTTGTAAATTGATTTTAGAGTAAAAGTTTTGAGTGCAATGTGATATATGTAAAACTAGAACTTTTTGCGAGTAGATAAAATGCGTGACACTGACACATCGTATATTAAATCTATTATTATGTTTTCTTTTCCTAATAAAGATTAGAAAAAGCTATCAATGATCCTATTTTTCAAGAGGACAAGTCCTATTTAGAGGAGAAATATAAGCAATACTACAAGCTCAATGGAATAAAGTTGGAATCGTCAAATTATATTAACTTTTTGCTCGAGTAAGAATCTTCACACACTGCGTAGATGTTCATCATAGAAAGCTCTTTGACAGCATTTGAAGTGTAAAAGATACAGCAGTGTCCAGGTCGCATTCTTCATGAGTTATCTTTCTTTTCTTTCTTTTCTTTCTTTATGGTCTGCAAAGGAAAAAAATCTTGATGTGAGATAGGTATCACAAGTTTCTCCTGTTTTGGACGAACCCAAGAAATTCTTGTCACAAAAGCACAAATATTTAATAGATGATTCATGTTCATGTTATCACTTTTATGTAGAATGCATCTGTCATGAGGGAGGAAAAATACTGATGAGGTCTTCTCTTGTATCATTGGTGATTAATTGAAAGATTGAGGTTAGTATTTTGGGGATTGGAGAAGAAACTTACCAAATAATGATTTCCAAACCTTAACAGTTGAAGTGCGTGGTTCTAGCCTCTCTCTCTTCAATGCTAGCGGAGTTTTCTCAAATGTCTCAAAGTACATGTACTTCAGCTGCTAGTTGGTCCTTTTATGGCTCCGAAGCTGAATTTACTATGGATAAATGTGGTTAAAGCTCTTATGTCGTGGTTGGGAGGAATGAGATAATTTTCGATAATAAACAACCCCCTTGGGCTGATTGTTTTTGAATATGCAAGATTAAGGGCTTCATCATGATGTTCTCTCTCCAAGTCTTTTTCTGTTTTATTTTACTAGATATATGTTTAAATTGAGATGTTTTTATTTTTCCATTTTAATTGTCTATTTATTTCCTACTTTTCTTCTCTCAATGGTGCGTTCTGTGTCACCATTGCTAAAATAAAAAATGGATAAAATTTTCATCATTGTTTTTTTTTCAATGACAGAGTGACAGCGAATACATGGCTGGTCAGCTTTCAGCATTTGGTTATTTGTTAAGTGAGAATCCTGAAGAGGCAGACTTGTGGCTCATAAATACGTATGATCTGTTCCTTCAAATTTTACTGTTTCTTATTTTGTCTTAATTTTGTAGAAATGTTCAGTGAAAATTTCTTTCTTAGTTGCCAGAAAACATAAGTTGCCTGTGAATACTTTCTAACGGTTTTTTGTGCCTCTAATGTGCCTTGTTGGCCTTAAGTCTACCATTTGTCTCTGCATCATTTATTCAAGTAAACTGTTCTCTTTCCCAGGTTGGTTATTACATCTTCTTAAGGACATTAACCTTTAACTTCCTCTAATCTACCTGGTGTCTTCTCTCATTATTGTTCTTTGATGTAGATAGCTGTGCATATATGTGCTTTGACACAGACATATATTTTTGCATAGACAGTCATGAATTTTTTTCCTTTTCTCCACTTATAAAAGAGACAATAGCCTGATAACTATTTTTTCAGTTGCACAGTTAAATCCCCTAGTCAGTCTGCTATGGACACTCTAATAACAAAATGCAAAAATGCAAAAAAGCCTCTTGTGGTAGCTGGGTGTGTACCACAAGGTAGTCGAGATTTAAAGGAGCTAGAAGGGGTCAGTATAGTAGGAGTCCAGCAGATTGACCGTGTGGTAGAAGTTGTGGAAGAGACACTGAAGGGTCATGAAGTAAGGCTATTGAATCGCAAGACACTGCCAGCGCTTGACCTTCCAAAAGTGCGAATTGTTTCCTGTTCTAGCCTTTTGAATCTTCTATTTGAGTTTGATTTAAGAAGCTTCTTTAACATATATTATCTTTGTCTTACCCTTTCGCCAACTATTAAAGAAGTTTATTTTGATTTTGAATTTTGAACAGGTGAGAAAGAACAAGTTCATCGAAATACTACCAATCAATGTTGGCTGTTTAGGTGCTTGCACTTATTGCAAGACAAAACATGCTCGTGGTCACTTGGGAAGTTACACAGTAGATAGCCTTGTAAGAACTTTAAACTAATGTATGGTTTTTGTTAAACATATGCCCCTTTTTTTCAGTTAGAAACTTAATGTTGTTCGATCTGAAGAAACATCTGCTAAAACTGTATTTGCAGGTAAATCGTGTAAGAACTGTCATCAATGAAGGAGTCCGGGAAATATGGTTGAGCAGTGAAGATACTGGGGCATATGGTACTTTACTCTACTTCCATAGAAATATATATCGTGTTGATTGAGTTATTTTATCAGAAGAGAACATTGAATCCATAGAAATATATAGAGTAGGATTTTCAATAAATGTTGATATCAATTAAGATTTATGGCCCAAAACCATCAAAATTGTGAATGAAATTATATTCTTCATCACTATGGACATCTCAATCCTTCCAATTATCATTTTTTAATGGATATGCATTATAAAAAATAGTTTAAAATTTTTTGAGGAATTAATTGCTTGCTTTTATTGTTGAGGAATATTCTCTCTTGGGGTCGAGGGGACCAATTATGGTTCTTTTTATGAAAATTAAAGTGTGCTCTCTCCCTCCCTCACTCACCTCCTTGTGGGGTTGGTGGACCAATTATGTTTCTTTCCTCCTCTCTCTTTCGGGAGACATCTCTTTCTTTATTCTTAGGTTGCTATACTTGAATCAATGATGATTTTGTAAGCTTCTTTCAATGCTGATTCTCTCCCTCTCTCCCCCAATCCACCATCTCACCCCCATCAAAAACCATGGCCAAACTCATACCACCTGTCCCTGACCATGGCAATAGACCCACCATTGTAGAAGAAAATTGTTACTATCAAAAGAAAGAAGTACTCTTAGACTTAGACAATAGATATCAAACGAATAGAGTAAAGGTCTCAGAATTTACGCAAGAAAGGCTTTCTTCATACCTCTACATTGGGAACCTTTGAAATGGTTGTTACTATCACACATACGTGTGCTCCTTTAAATGGGAAGTTCTTTAGGGAAGAGTGGTTTACCCAATGGGTTATCTGAGTTGAAAAAATTGCAAACAAGAAAGATACTCTTGTCGAGGTTGTCCTTCTACATACCAAGGAGGGTTAAACAAACTCTTTGTACCTATTGATGCAGAATGAAAAGGCTGGGATGTTTTTTATAATCTCATAGCTAACTACCCTGCCCTACCATTAAAGGTGGTTTACCAACCTAAAATCCTCCATTCTTCAACAAGACTACTAATGAACCTGCTCTTAAACCCTTTTACCTACCGAAAATTCTTCCCCCACTCCACTATCATTAAATTGTAGTCTTGTTGAAGGATTGAGGGTTTAGGTTGGTCTGCTTATTGGGATTTTGCTATGATTGTCACTAGAAGAAACTTTCATGATAGTTTGTACAAGATTTTTAATGGCCCTATTTTTCTTAATAAAATAGAAATGTGGTATGCCTAATATGTGCAAAAATTCATTAGAACAATCACAAGTAAATGTTTCCATGGACTTAAACGTTTGCAAAATTCATTTAAAATGAAGGTAAAAGTGATGTTAACAATAACAACAGAAATCAAAATAGCAAGAAATTCAAATGAATGGAAACACCCTGATCTAATCTGAAGGCTACAACGTTAAATGCAAATGCGACTACCCTAACTTCATTCCCATGGCCTCCAAAACGACGCTATCATCCGTACAAAGGTGCCTTACCTTTACCTGAGAAAGAAGTAGCACGTGATTTGAGTATTTAAAGGAATACTAAGCAAGTAGCCTTATTGTTGAGGTCGTGAAATATAATCACATGTAATCATGTTGGGACCTATTTTTTCATAAAAACATCATGGTCTTGAACTCTACTTTTCAGTTCGAACGAAAGTGGATGTGTAGTATTTCTCCACACGGCCCACCACGCGCACATGGACCCTCCAAGCGGGCTTGTATACATACCTCTTGGGTTTGGCCTTTGACTAGTGCACGCTCACTCTGTCGGAGCTACAAAGGGAAAATAGCTTGCATAATATCATGGGGTACATAAATATTGTAATGTACGCACTCATACAAATCATAACGGGGAAGCATCTTGATGCACGTGACATACAAAATGCATGAGGTTCCATAGTATCAAATCATGACATATACTTATGATGTAAGTTGTACGTCCCTTCTTTTCATAACATAGCATTCTTATGAGACATGCAATGTATACTAAGCATATCTTTCATGGTTAATATGTATGGTTTATCAATTTCTCGTAGGCATAGAGTGAACATGCTAACATAATATATAGTATGACATATGGAGGTTCATGCATCATATAATGTAATTAGGTAGAGTCAATTGAGGGTTCAACAATCACAATGTGGATGAGTTGTCAAACTAAAAAGTCAAGGAAAAGCCAAGCAGTAACACTCTAACTCAACCAGGAATCATGCCAAAGAGAGGTTGAGAGACCATTACCAAGACTTCTCTGAGCATGATTAGCTAGGTCAATAGATGAACAAATAAAACCAAGATACGAACTAAACCCCTCCAAATATGTCGAGACAAAAAACAATTGTTCTCTGAAGAATAATTATTTTGTTATTCTTCTTTCCCTTGCTTTTCCGTGCTTTTATTTGTATTTTAATATTTCAACAATAGGTTGGTCTAATGATCAAAACAAAAACTATCCATTGAGATACAATCGAAGTCATATATAATCTTCAATATATCTCTAAGTCTAGATCTTGTATATTTGTTTGGGTGGTCGTTTGTAAGATTAGCAAAAACACGACACAGTGACCTATAGGTTGAAGAGAACCAATGATTGGTTTTGTATCATGAATACTATGATAATTTTCAATATTGAATACATTTTTTCTAATAATATGAAATTTGAACCTTGAAATAAGGCATACATATTATCCAAGGATCTCTAAGATCATAAAATATTTGTCCTTGGCTATAAAGTATCATCACTTGTAAAAGGGCCAAGTATTGATGTCACACTTATATCGCACTTCGACACGTTGTCGACACATGTTTGACACATTGTTTTGCATGCTATTATTGTTTTTTATATTATTTTTCATTTTTTGGCATGGCAAGGACATGCTCGGACACAGTTAGGGACACACAAAGACACGGTTAAGACATGTAATGCCAATTTTTTTTCAAGTTTCAAGTCTTTTAAGATCATTTAAAAGAAAGCCCACTCCGCATATAGACCTCAAAGTATTAGTGTTTCTTACCATCATCTTCCAACCTCAAATCTTGCCTCCACGACAAGAAAAAAAATCCTGCACAACTGCTCATCAACAACAACAATGAATGACAGTTGTCGGTTGTCGTTGATCACTAGTGGCCATTGGTGAAGAGGACAACGATGGTGGCCGCGGATATAGAAAGGCTTTTTAGAAATTACTTGTGGAAAGATAGTGCACACCTTGTTTGTTGGAATATTCTAAACCTCCCAACAGAAAAGGGAGGTCTCGGTCTTCTCTCGATAAGGAAGAAGAACACAGCTCTCCTCGCCAAATTGATATAGAGATATCATCACGAAAAAAAGGCCTTATGGAGAAATCTTATAAAGGCTAAATATACTCCTACATCAAACAAAGATCACCTCCCTCCATCTTCTACAAAAGGGCCTTGGAAGTACATAAAGAAACATCAAAATCTCATCACCCACCGAACTCGCCATAAGGTGGGGAATGGGGGAAGCACATCATTTTGGACCAACCCATGGATTGAAAACACCACGCTAGCTTTGAAGTATCCACTTTTGTACAGGCTTTCTCACAACAAAAAAGCCACGATTAAAGAAACGTGGAATGTTGTCAACAAATTTTGGGACTTGAAGCTTGGGAGGAATCTAAAGGATAATGAAGCAACAGAGTGGGCTGAATTAAGCCTTGACCTTGCCCCTGTGGTATTGTCAAACAAAGAAGACTCACTAACTTGGCTCCCCAATGCAGACGGGGTCTTTTCTACAAAATCCTTGATGATGGACATGGGGAAAAAAAGTAGAAGCAATAAATCCCACGCTAGCAAAGACATTATGGAAAGGACATCAACCTAAAAAGGTGAAGTTCTTCCTTTGGGAAATAGCGCATAAAGCCATCAGCACAAGTGAAAATCTTCAAAAAAGAATTCCTTACATCACTCTCTCTCCAAATTGGTGTCCACTATGCAAGAAAGCAAACGAATCACAAAGTCACTTGTTTATGGAATGCACATACACTCAAAATTTATGGCCAACGATTCTCAATATATTCGGATGACATCTCACATTTCCTAGGGAGGTAAAGGATTTTTTTGGATATGGCCTTAACGTGCCACCCTTTCAAGAACGCAAAAGCCCTATTATGGAAAAACCTCATCATGACTTTCTTTTGGAATCTGTGGAAAGAAAGAAATCAGAGAATATTTGCAGAAACGACATAGACCTACACAAAACTTTTCGACAATGTTGTTTACCAAGCTATATCTTGGTGTAAACTGTCTAATATTTTTACTTCTTATAGTTATACCTCCCTCGTTGCAAATTGGGAAGGTCTTTTGTAAACATCATGGATTATACATCCCTCTTGTAAATTTCACTCATCAATGAAATTGTCTCTTATAAAAAAAAAAANAAAAAAGACAAGGATGGGGAAAATATGTAGTATGTAGAAGACTCGTTTTGTTTGATTCTATCTTGTAGTTTTCTGTGTAAGACTTGTTGAACATTGAATATTGTTGATGTTGAAGTTTTTTTGTGTAAGACTTGTTGAATATTGGATATAGTTGATGTTGTTTGTTGATTGTTATCTTATCATTATCTTTTTAATGAATTTTATGGATGTTCTTTTGATGTTTAAATGTAGAACGTTACATTAGAAGTAATGAATATTCATAACATGTCAAATTATACACATTTTAAAAAATAAAACAAATGTTTCCCCTATGTGTTGTGTCCTACTTTTTTGGAAATTAATGTGTCGCCTGTTCATGTCGTGTCGTATCCACGTTACATGTTCTTGCCTGTGTTTTTTTTTTTTGTCCTTGCTAATATCATATGATGAAATTTCAGGCCGTGACATTGGGGTCAATCTTCCAATTTTGTTAAATGCAATTGTTGCCGAGCTTCCTCCTGATGCAAGCACAATGCTTCGGATTGGGATGACAAATCCTCCTTTCATTCTTGAGCATTTGAAAGAGATAGCTAATGTTTTGTGTCATCCATGTGTATACTCCTTTTTGCATGTGCCAGTTCAATCTGGGAGTGACACAATCTTAAGTGTAAGTATTGTCTATACCCAAATTTTCTTAGATTTGTTTTTAGTGACTTGAGGCCAGTACAGTTTTGAGGAAGGAAATTACTTGTAGACCTAATCATTACATTTTTCTCTCAGGCAATGAATCGAGAATATACAGTCAGTGAGTTCAGGACTGTAGTAGATACCTTAACTGAGCTTGTTCCTGGGATGCAGGTCGCAACTGATATAATATGTGGATTCCCTGGTTAGTAAGTGGGTTTGAGGGGATTGATCCCTATACTTCTATTGTTGTCTCTCTATTGGTTTGCATGGGTTTTTATTCTTTGCATACTGTTTTGGATGGGAAATGAAACTTTCACCTTCATTGATTTCCTTGTTGCAAAACACCAACTGCTGAAACAGTGTAATCTATAAAACTATTGTTCTCCTCAAAGATGCAATATTTGAGCTATTACTTACAAACATTTTAAAATATTGATTTTCATAATGGTCAACTATATTGATTATTATGAAAATGTAACCCTTTGGCCTTGTGGGATACCTCATCTCCCCTCTTTTTGTAATAGTTCCTCTTGTTAATTTTACTGTCTCGTTTCCTTGTTCAATTTTTTTTTTTTTTTTTTTTTTTAAGAAAATAACTGAAAAATACTAATGTTCAAGTATCAGTGGTTTGTTTTGGTCGCTATGCGGCAGTGTGAAAATTATCTGTTAGGTTTGTCAGGGCCTTTATCCCCCATTTTTTTCTTTGTATCATCTTGAATGTTTATCTCCTCCAATGTTGTATACAATATTAATTCGCATTTTGCGGAGCATAATATCTTAAAATTTTATTACATGTGAATTATTGCCAAACTTTTGTTAATTCTGTGGCTTTTCAGGGGAAACGGATGAAGATTTTTGTGAAACTATCAACCTTATTAAAGAGTACAAATTACCCCAAGTTCATATCTCACAATTCTATCCTCGACCAGGTATGGCATCATGTTCAAGCCTCCTTATTTCTTTGCATAGACTTTAACTAAAGAAGGTAACAGTTAGACTTGAATGCTAATGACTAGCTTAATAATCCTCTATCTCAAATTAGGATGAACCTTGTGCTATTCCTTGTGTATTATTCAGGAGACACATCACATGCTTAAAATGTTTATTTCCCAGGATGTGGCAATAAGATTACCTAGGAAGCATATATATATATATATATATATATATATATTTTTTTTTGGTTAATCCAAACACTTTTATATCCCTTCTCATTTAGTTTTCTTGTTGTTGCTTGCAACTTATTAGGACCAAGGTTTTTTAATTTATTAATTTGTCTAATTTTTTTTTCTTAACATGACTGAGAAAAATAGAAGAAAGCAAAAGGGCATCAAAAAACAACCTAGAAAAAAATCCTGAAAAAAAAAAACCTAACTAAATATAAAGGGATTCAAACCAAAAGAATAAAGCCAAAGAGACATTGAACGAAGAATCACATTTTGTTTTGTTTGGAAGGTTTTTTTTTTGTTAGGATGGTAGAAACTTAACATTTCTTTGTCTAATTTTAAAGAGCAACTGCTACTTATGGATCCCTCTATAATACAAACAACCTATTTTATACTTATTCAGATGAAACAGTGTGATACTAGCCACAACAAATGATTGAATGACTAAGATGAATTATTCATGCATGTGCAAAATCCAGCAATCAAACAGTTTGCCCATGTTACCTTAGAAGAACCCTGGCTGTAGCAATTTCTTTCATTAAATTGATTGTCTCGGATATATCTGGCTAGACTAGGTTTTGGAACTTCTTTGATTCCAAGTAAATAGAATGTGATTACTTAAACTAATGTTTCCAGCCGTCAACTATTTAAAATGAAAAAAACAGTATTCTTCTCTCTTGTCCTTCAGGGACACCTGCTGCTAGGATGAAGAAAGTTCCAAGTGCTATTGTCAAGAAACGAAGCCGCGAGTTGACTTCGGTTTTTGAAGCTTTTACTCCATATAATGGAATGGAGGGCAGAGTGGAAAGAATTTGGATTACAGAAATTGCAGCTGATGGAATTCACTTGGTAAGAAAGCGATGCTGTCTAGTGTATTTGTTTCTAATTCTTCCATGTATTTGCCTACAGTTGTTTCAGGTGGCTGTTTCTACACAAAATAGTTAGGACAATGGTGAGAAAAATTGTTTGGATCAGTTTTAGGCCCAAATTTGAGACATCAAGGCTGTTTAATTGACCTTTTAGCTAGTGTAAATGAAATTGTGTTTACAGTAACAAACAAGAAAAGGGAGAAAATAGGATAAAGAAAAAACTGGTAAAATTCACTGTTCAACATTTTGAGAATCCATATAAAATTTTAGACTCAGTCTGCATGCCTAACAAATTATAGAAAATATTTATTGGAAGGAGTAAAAAGAAAGATAGAAAATATCTACGGGCGGAGTAATCAATCAATAAACATTTTAATTCACCATGTTCTACACGAGGAAAAACCCAGCTGTACCATCTATTGGCCTTTAAGTGAAATAGAATTAGATACCGTCACCAAAATTTGGATTTCAAGACATTAAGGACTTGCATTTCTACCTAGCATTTAATAACTAAGTTATTTTTTCTTTTTAACAAGAAACAAAATATTTCATTGAATTAATTAAAAGAGTCTAGCAAATATCTTGACCATTGAGAAACCTTCCATTATTCCATCTCAAAACAATCAACCCAATATTTAAATATAAGTAGGTTCTATTAATCTTAGTTAATTTTAAACATTCTTCAAAAAAAGGATTTTCTTCAAACATGTCTTATTTGACGTTGCTTCTTTAGACAATATGTAATTGGCTTGTTCATGAGCGAGTTAAATTTTATTCATTCTTTCATATACGTATATAAAGTATACTATTTACATTAGCTTAGACCATTCCTAACCCAAGGCCTGGTCTGTGGAATGCATGTTCTCTGTACACTCTACACTCTTGCAGTTAAGACCATCCAAGTTAACACTATCTTGTGGTGAACTTGGATAGGAATAAATAGTAAACCTTTAGAAGGTAAGCTTAGATCGCGAGCTGAAGTATATGGGCTGAAGTCTTTGGTTATGTTAAGTTTTTGATGTAGTAGATTAGCCGACTAGGGATAATCTTGTAATTTTTGGCTGAGTTTGTTAATTGTTAGTTGTTAGTTGGGTTTAGGTTAATGGTTTTGGTAGTTAAATTAGTTTGTTAATTGATTGCTTTGTTTGTAACTGGGTTTTTTACTTAGCTTGTCAGTTGGCATCCAGAGGATAGTCTCTTGTAAAGACTTCGGAATAGTAATTAAAAAAAATGGTTATCCTTGGGTTCTATTTTTTGGTTGCGAGGTTGCATCATTTTTTGTATAGAGAACGGAGAAGTAGCTTGGCCAAGAAAGATGACGAGCAAGAGTCTCGAATGGTAGGGAATGAAAGTTCATTTGGTCCAAATGGTAGGAAATGTGTGGAAAATCAGTTGCAATTGTACTGACTTAGTTGATTCACATTCACTCAAGCCTGAGATTTTCACACGTAAGAAAGTAGTGAAAAACATCAATATTCCATGGGAGAGATCAGAAATCAGTCGTTAATTTATAAGTTCTGCAAAATTTCACCTTTGAAATTTAACAAAACTTTTAGAAAAGGGTCAAATTCTCTCGGACAATTGATTGAAGAAAATGTTATTATCTATGGATCTAGGTTTGGAGAGATCTTGGGAGGAAACTTGTGGTAGGCTAGAAAGGGACAACATAATTTTTAGCGAGCGGAGAGATTTTGAAGGAAGTTTGGGAGGTTGCTTGGTTTGCCTTCCCGTGGGAATCCTTCCATTGGAGTTTCTTTTATTGTTAGCTTTGTTTCATTCCTTTGAATTGGAATACCATCCTGTAGTTAATTGTTAACTGGTTCTTAATCAATTTCAATTCCCATATGCATTTTAGTGCATCATGGAAGTGAACTTCCTTTTCTGAGCCATCTCATAAAACTGCTGTTTTTACATACTTTTCATCATCTAATGTTTCAAAGAACTGTTGTTTCTTTTTCGATAAGCTCAATTTATATTGCTCCCCCCCAGGTTGGCCATACCAAGGGATACATACAGGTGCTAGTAATTGGCCATGAAGCTATGCTGGGAACTTCTGCTTTGGTGAAGATAACATCCGTTGGAAGATGGTCTGTTTTCGGGGAAGTGATTGAAACCATCGTCACCAAACATCATAAAACAACTGCTCTCGAAGACATGCCGATTCAAGCCAAGGTTCCTCCCTGCTCTAATTCTCATGAAACATGTGCATGTTCCGTGGAACCCGAAGCTTGTGCTTGTGGACCAGAAAGCTGCTGCAAAGGACCAGTTGCTCTGGATGAGAAAGATGATTCTTCAAGAAATATCCCATTGCCTGAAGAGCCCAAACGGAAGAATCTGGTTGAGTGGCTATTGCGGAAACGGAAGAGCCATGTGCCCAAAAGGGAGGAAGGGGAAAGTCCGGTAGTGTCCGGGAGAAAGCAGGCATCGGCTGGAGGAAGCATGAGTGAGTGGGGCGTCGTCGATAGGATTCTTGTTGGAGGAATACTAATCAGTATCTTCACAATTGTTGGTCTGCTGTTTCATCTTGGATCCACGACACTCTCCTCCAGTTGATGATATTAATGATATTATACAATGCTAATTTGTATCGGACCATATTTAAGAAAGTTTGTAATGAGAGCTATAATCCATCATATCTTCATGTTCCTTTTCCAACTTTCATCCATGGAGATCGTGTTTTGACGGTCACAATTTCAATTGTAATTTGAGAGCGATTTTGTTTCAATTTCACAGTTCTCCCTCTAGAGCTTCATCTACTTCCATGACTTTGAATCTACTAGAATACATGGAATAAACTAATTAACTATTGAAGATAGATTCATATTTACAAATTTTGGATGGATG

mRNA sequence

AGAAGAAGGAGCAGCAGCAGAAGCAGCGAAGCACCGCGGCTGCCCTTCTCGTCTCTTCAGCGGAGGAAACTGTAAGCCATGGAAGACATAGAAGACCTGCTCATCGGCGGCGGCGGCGGCGGTGGAGCTCCCCCTGGTTACCGACTTCCAATATCCGCAGTCGGTGTAAAGTCGAAGAAAAAGAATAAGCTGAAACCTAATCCTGATGCTGATGATTCCAGCAGCGGGCTTTTTCCAACCCAAAATCTACTGGCACCTAAGATTCCAGGCACTCAGACAATTTATGTTAAAACCTTTGGATGTTCTCACAACCAGAGTGACAGCGAATACATGGCTGGTCAGCTTTCAGCATTTGGTTATTTGTTAAGTGAGAATCCTGAAGAGGCAGACTTGTGGCTCATAAATACTTGCACAGTTAAATCCCCTAGTCAGTCTGCTATGGACACTCTAATAACAAAATGCAAAAATGCAAAAAAGCCTCTTGTGGTAGCTGGGTGTGTACCACAAGGTAGTCGAGATTTAAAGGAGCTAGAAGGGGTCAGTATAGTAGGAGTCCAGCAGATTGACCGTGTGGTAGAAGTTGTGGAAGAGACACTGAAGGGTCATGAAGTAAGGCTATTGAATCGCAAGACACTGCCAGCGCTTGACCTTCCAAAAGTGAGAAAGAACAAGTTCATCGAAATACTACCAATCAATGTTGGCTGTTTAGGTGCTTGCACTTATTGCAAGACAAAACATGCTCGTGGTCACTTGGGAAGTTACACAGTAGATAGCCTTGTAAATCGTGTAAGAACTGTCATCAATGAAGGAGTCCGGGAAATATGGTTGAGCAGTGAAGATACTGGGGCATATGGCCGTGACATTGGGGTCAATCTTCCAATTTTGTTAAATGCAATTGTTGCCGAGCTTCCTCCTGATGCAAGCACAATGCTTCGGATTGGGATGACAAATCCTCCTTTCATTCTTGAGCATTTGAAAGAGATAGCTAATGTTTTGTGTCATCCATGTGTATACTCCTTTTTGCATGTGCCAGTTCAATCTGGGAGTGACACAATCTTAAGTGCAATGAATCGAGAATATACAGTCAGTGAGTTCAGGACTGTAGTAGATACCTTAACTGAGCTTGTTCCTGGGATGCAGGTCGCAACTGATATAATATGTGGATTCCCTGGGGAAACGGATGAAGATTTTTGTGAAACTATCAACCTTATTAAAGAGTACAAATTACCCCAAGTTCATATCTCACAATTCTATCCTCGACCAGGGACACCTGCTGCTAGGATGAAGAAAGTTCCAAGTGCTATTGTCAAGAAACGAAGCCGCGAGTTGACTTCGGTTTTTGAAGCTTTTACTCCATATAATGGAATGGAGGGCAGAGTGGAAAGAATTTGGATTACAGAAATTGCAGCTGATGGAATTCACTTGGTTGGCCATACCAAGGGATACATACAGGTGCTAGTAATTGGCCATGAAGCTATGCTGGGAACTTCTGCTTTGGTGAAGATAACATCCGTTGGAAGATGGTCTGTTTTCGGGGAAGTGATTGAAACCATCGTCACCAAACATCATAAAACAACTGCTCTCGAAGACATGCCGATTCAAGCCAAGGTTCCTCCCTGCTCTAATTCTCATGAAACATGTGCATGTTCCGTGGAACCCGAAGCTTGTGCTTGTGGACCAGAAAGCTGCTGCAAAGGACCAGTTGCTCTGGATGAGAAAGATGATTCTTCAAGAAATATCCCATTGCCTGAAGAGCCCAAACGGAAGAATCTGGTTGAGTGGCTATTGCGGAAACGGAAGAGCCATGTGCCCAAAAGGGAGGAAGGGGAAAGTCCGGTAGTGTCCGGGAGAAAGCAGGCATCGGCTGGAGGAAGCATGAGTGAGTGGGGCGTCGTCGATAGGATTCTTGTTGGAGGAATACTAATCAGTATCTTCACAATTGTTGGTCTGCTGTTTCATCTTGGATCCACGACACTCTCCTCCAGTTGATGATATTAATGATATTATACAATGCTAATTTGTATCGGACCATATTTAAGAAAGTTTGTAATGAGAGCTATAATCCATCATATCTTCATGTTCCTTTTCCAACTTTCATCCATGGAGATCGTGTTTTGACGGTCACAATTTCAATTGTAATTTGAGAGCGATTTTGTTTCAATTTCACAGTTCTCCCTCTAGAGCTTCATCTACTTCCATGACTTTGAATCTACTAGAATACATGGAATAAACTAATTAACTATTGAAGATAGATTCATATTTACAAATTTTGGATGGATG

Coding sequence (CDS)

ATGGAAGACATAGAAGACCTGCTCATCGGCGGCGGCGGCGGCGGTGGAGCTCCCCCTGGTTACCGACTTCCAATATCCGCAGTCGGTGTAAAGTCGAAGAAAAAGAATAAGCTGAAACCTAATCCTGATGCTGATGATTCCAGCAGCGGGCTTTTTCCAACCCAAAATCTACTGGCACCTAAGATTCCAGGCACTCAGACAATTTATGTTAAAACCTTTGGATGTTCTCACAACCAGAGTGACAGCGAATACATGGCTGGTCAGCTTTCAGCATTTGGTTATTTGTTAAGTGAGAATCCTGAAGAGGCAGACTTGTGGCTCATAAATACTTGCACAGTTAAATCCCCTAGTCAGTCTGCTATGGACACTCTAATAACAAAATGCAAAAATGCAAAAAAGCCTCTTGTGGTAGCTGGGTGTGTACCACAAGGTAGTCGAGATTTAAAGGAGCTAGAAGGGGTCAGTATAGTAGGAGTCCAGCAGATTGACCGTGTGGTAGAAGTTGTGGAAGAGACACTGAAGGGTCATGAAGTAAGGCTATTGAATCGCAAGACACTGCCAGCGCTTGACCTTCCAAAAGTGAGAAAGAACAAGTTCATCGAAATACTACCAATCAATGTTGGCTGTTTAGGTGCTTGCACTTATTGCAAGACAAAACATGCTCGTGGTCACTTGGGAAGTTACACAGTAGATAGCCTTGTAAATCGTGTAAGAACTGTCATCAATGAAGGAGTCCGGGAAATATGGTTGAGCAGTGAAGATACTGGGGCATATGGCCGTGACATTGGGGTCAATCTTCCAATTTTGTTAAATGCAATTGTTGCCGAGCTTCCTCCTGATGCAAGCACAATGCTTCGGATTGGGATGACAAATCCTCCTTTCATTCTTGAGCATTTGAAAGAGATAGCTAATGTTTTGTGTCATCCATGTGTATACTCCTTTTTGCATGTGCCAGTTCAATCTGGGAGTGACACAATCTTAAGTGCAATGAATCGAGAATATACAGTCAGTGAGTTCAGGACTGTAGTAGATACCTTAACTGAGCTTGTTCCTGGGATGCAGGTCGCAACTGATATAATATGTGGATTCCCTGGGGAAACGGATGAAGATTTTTGTGAAACTATCAACCTTATTAAAGAGTACAAATTACCCCAAGTTCATATCTCACAATTCTATCCTCGACCAGGGACACCTGCTGCTAGGATGAAGAAAGTTCCAAGTGCTATTGTCAAGAAACGAAGCCGCGAGTTGACTTCGGTTTTTGAAGCTTTTACTCCATATAATGGAATGGAGGGCAGAGTGGAAAGAATTTGGATTACAGAAATTGCAGCTGATGGAATTCACTTGGTTGGCCATACCAAGGGATACATACAGGTGCTAGTAATTGGCCATGAAGCTATGCTGGGAACTTCTGCTTTGGTGAAGATAACATCCGTTGGAAGATGGTCTGTTTTCGGGGAAGTGATTGAAACCATCGTCACCAAACATCATAAAACAACTGCTCTCGAAGACATGCCGATTCAAGCCAAGGTTCCTCCCTGCTCTAATTCTCATGAAACATGTGCATGTTCCGTGGAACCCGAAGCTTGTGCTTGTGGACCAGAAAGCTGCTGCAAAGGACCAGTTGCTCTGGATGAGAAAGATGATTCTTCAAGAAATATCCCATTGCCTGAAGAGCCCAAACGGAAGAATCTGGTTGAGTGGCTATTGCGGAAACGGAAGAGCCATGTGCCCAAAAGGGAGGAAGGGGAAAGTCCGGTAGTGTCCGGGAGAAAGCAGGCATCGGCTGGAGGAAGCATGAGTGAGTGGGGCGTCGTCGATAGGATTCTTGTTGGAGGAATACTAATCAGTATCTTCACAATTGTTGGTCTGCTGTTTCATCTTGGATCCACGACACTCTCCTCCAGTTGA

Protein sequence

MEDIEDLLIGGGGGGGAPPGYRLPISAVGVKSKKKNKLKPNPDADDSSSGLFPTQNLLAPKIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTVINEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIICGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHEAMLGTSALVKITSVGRWSVFGEVIETIVTKHHKTTALEDMPIQAKVPPCSNSHETCACSVEPEACACGPESCCKGPVALDEKDDSSRNIPLPEEPKRKNLVEWLLRKRKSHVPKREEGESPVVSGRKQASAGGSMSEWGVVDRILVGGILISIFTIVGLLFHLGSTTLSSS
BLAST of CmaCh01G008300 vs. Swiss-Prot
Match: CDKAL_DROPS (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Drosophila pseudoobscura pseudoobscura GN=GA19679 PE=3 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 1.9e-142
Identity = 263/497 (52.92%), Postives = 344/497 (69.22%), Query Frame = 1

Query: 1   MEDIEDLLIGGGGGGGAPPGYRLPISAVGVKSKKKNKLKPNPDADDSSSGLFPTQNLLAP 60
           ++DIEDL+         P        +V V++KK+ ++KP  DA++  +   P   +   
Sbjct: 13  IDDIEDLI---SAEDVKPQERYQNKKSVTVRAKKRVQIKPETDAEEKPT---PRPTIYES 72

Query: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSA 120
            IPGTQ ++VKT+GC+HN SDSEYMAGQL+A+GY LS   +EADLWL+N+CTVK+PS+  
Sbjct: 73  VIPGTQKVFVKTWGCAHNNSDSEYMAGQLAAYGYKLS-GKDEADLWLLNSCTVKNPSEDT 132

Query: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180
               I    +  K +VVAGCVPQG+     L G+S++GVQQIDRVVEVVEETLKGH VRL
Sbjct: 133 FRNEIESGMSNGKHIVVAGCVPQGAPKSDYLRGLSVIGVQQIDRVVEVVEETLKGHSVRL 192

Query: 181 LNRKTLPA-------LDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSL 240
           L  K +         L LPKVRKN  IEI+ IN GCL  CTYCKTKHARG L SY  + +
Sbjct: 193 LQNKKVHGRRVAGAPLSLPKVRKNPLIEIISINTGCLNQCTYCKTKHARGDLASYPPEEI 252

Query: 241 VNRVRTVINEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPP 300
           V+R R    EG  EIWL+SEDTGAYGRDIG +LP LL  +V  +P     MLR+GMTNPP
Sbjct: 253 VDRARQSFAEGCCEIWLTSEDTGAYGRDIGSSLPELLWKLVEVIPEHC--MLRVGMTNPP 312

Query: 301 FILEHLKEIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGM 360
           +ILEHL+E+A VL HP VY+FLHVPVQSGSD++L  M REY   +F  VVD L E VPG+
Sbjct: 313 YILEHLEEVAKVLQHPRVYAFLHVPVQSGSDSVLGEMKREYCRKDFEHVVDFLRERVPGV 372

Query: 361 QVATDIICGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKR 420
            +ATDIICGFP ET+EDF ET+ L   Y+ P + I+QF+PRPGTPAA+M+++P+ +VKKR
Sbjct: 373 TIATDIICGFPTETEEDFEETMTLCGRYRFPSLFINQFFPRPGTPAAKMERIPANLVKKR 432

Query: 421 SRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHEAMLGTSAL 480
           ++ LT +F ++ PY    G +  + +TEI+ D +H VGH K Y QVL+   + +LGT   
Sbjct: 433 TKRLTDLFYSYEPYAQRVGEMYTVLVTEISHDKLHYVGHNKSYEQVLLPMRDNLLGTRVH 492

Query: 481 VKITSVGRWSVFGEVIE 491
           V+ITSV ++S+ GE+++
Sbjct: 493 VRITSVSKFSMVGEILD 500

BLAST of CmaCh01G008300 vs. Swiss-Prot
Match: CDKAL_XENTR (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Xenopus tropicalis GN=cdkal1 PE=2 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 3.0e-140
Identity = 248/467 (53.10%), Postives = 331/467 (70.88%), Query Frame = 1

Query: 31  KSKKKNKLKPNPDADDSSSGLFPTQNLLAPKIPGTQTIYVKTFGCSHNQSDSEYMAGQLS 90
           + + KNK++      DS+             IPGTQ I+++T+GCSHN SD EYMAGQL+
Sbjct: 38  RKRNKNKIQEEEPPADST-------------IPGTQKIWIRTWGCSHNNSDGEYMAGQLA 97

Query: 91  AFGYLLSENPEEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKE 150
           A+GY ++E PE+ADLWL+N+CTVKSP++      I K + A K +V++GCVPQ     + 
Sbjct: 98  AYGYSITEQPEQADLWLLNSCTVKSPAEDHFRNSIKKAQEANKKVVLSGCVPQAQPRQEY 157

Query: 151 LEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPA-------LDLPKVRKNKFIEIL 210
           ++G+SI+GVQQIDRVVEVVEET+KGH VRLL +K           LDLPK+RKN  IEI+
Sbjct: 158 MKGLSIIGVQQIDRVVEVVEETIKGHSVRLLGQKKDNGKRLGGARLDLPKIRKNPLIEII 217

Query: 211 PINVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTVINEGVREIWLSSEDTGAYGRDIG 270
            IN GCL ACTYCKTKHARG L SY V+ LV+R      EGV EIWL+SEDTGAYGRDIG
Sbjct: 218 SINTGCLNACTYCKTKHARGELASYPVEELVDRAAQSFQEGVCEIWLTSEDTGAYGRDIG 277

Query: 271 VNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIANVLCHPCVYSFLHVPVQSGS 330
            +LP LL  +V  +P  A  MLR+GMTNPP+ILEHL+E+A +L HP VY+FLH+PVQS S
Sbjct: 278 TDLPTLLWKLVEVIPEGA--MLRLGMTNPPYILEHLEEMAKILNHPRVYAFLHIPVQSAS 337

Query: 331 DTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIICGFPGETDEDFCETINLIKEYKL 390
           D++L  M REY +++F+ VVD L E VPG+ +ATDIICGFPGETDEDF ET+ L++EYK 
Sbjct: 338 DSVLMDMKREYCIADFKRVVDFLKERVPGITIATDIICGFPGETDEDFKETLKLVEEYKF 397

Query: 391 PQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIA 450
           P + I+QFYPRPGTPAA+M++VP+ + K+R++EL+ +F +++PY+   G  + + +TE +
Sbjct: 398 PSLFINQFYPRPGTPAAKMEQVPAHVKKQRTKELSQLFHSYSPYDHKIGEEQHVLVTEES 457

Query: 451 ADGIHLVGHTKGYIQVLVIGHEAMLGTSALVKITSVGRWSVFGEVIE 491
            D  + V H + Y QVLV    A +G    VKI   G+  + G+ ++
Sbjct: 458 FDSQYYVSHNRFYEQVLVPKDPAFVGKMVEVKIFEAGKHFMKGQPVQ 489

BLAST of CmaCh01G008300 vs. Swiss-Prot
Match: CDKAL_DROME (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Drosophila melanogaster GN=CG6550 PE=2 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 2.5e-139
Identity = 264/521 (50.67%), Postives = 347/521 (66.60%), Query Frame = 1

Query: 1   MEDIEDLLIGGGGGGGAPPGYRLPIS-AVGVKSKKKNKLKPNPDADDSSSGLFPTQNLLA 60
           ++DIEDL+          P  R      V V++KK+++++     ++      P   +  
Sbjct: 13  VDDIEDLI----SADDVKPRERYENKKTVTVRAKKRSQIRLESQEEEEK----PKPTIHE 72

Query: 61  PKIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQS 120
             IPGTQ ++VKT+GC+HN SDSEYMAGQL+A+GY LS   EEADLWL+N+CTVK+PS+ 
Sbjct: 73  SVIPGTQKVFVKTWGCAHNNSDSEYMAGQLAAYGYRLS-GKEEADLWLLNSCTVKNPSED 132

Query: 121 AMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR 180
                I       K +VVAGCVPQG+     L G+S++GVQQIDRVVEVVEETLKGH V+
Sbjct: 133 TFRNEIESGMRNGKHVVVAGCVPQGAPKSDYLNGLSVIGVQQIDRVVEVVEETLKGHSVQ 192

Query: 181 LLNRKTL--------PALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVD 240
           LL  K            L LPKVRKN  IEI+ IN GCL  CTYCKTKHARG L SY  +
Sbjct: 193 LLQNKKKVHGRRVAGAPLSLPKVRKNPLIEIISINSGCLNQCTYCKTKHARGDLASYPPE 252

Query: 241 SLVNRVRTVINEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTN 300
            +V R R    EG  EIWL+SEDTGAYGRDIG +LP LL  +V  +P     MLR+GMTN
Sbjct: 253 EVVERARQSFAEGCCEIWLTSEDTGAYGRDIGSSLPELLWQLVEVIPEHC--MLRVGMTN 312

Query: 301 PPFILEHLKEIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVP 360
           PP+ILEHL+E+ANVL HP VYSFLHVPVQSGSD++L  M REY   +F  VVD L E VP
Sbjct: 313 PPYILEHLEEVANVLQHPRVYSFLHVPVQSGSDSVLGEMKREYCRQDFEHVVDFLRERVP 372

Query: 361 GMQVATDIICGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVK 420
           G+ +ATDIICGFP ET++DF ET+ L  +Y+ P + I+QF+PRPGTPAA+M ++P+ +VK
Sbjct: 373 GVTIATDIICGFPTETEDDFEETMTLCAKYRFPSLFINQFFPRPGTPAAKMDRIPANLVK 432

Query: 421 KRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHEAMLGTS 480
           KR++ LT +F ++ PY    G +  + +TE++ D +H VGH K Y QVL+   + +LGT 
Sbjct: 433 KRTKRLTDLFYSYEPYADRVGEIYTVLVTEVSHDKLHYVGHNKSYEQVLLPMRDNLLGTR 492

Query: 481 ALVKITSVGRWSVFGEVI--ETIVTKHHKTTALEDMPIQAK 511
             V+ITS  ++S+ GE++  E   T+  K   L ++ +Q +
Sbjct: 493 VHVRITSASKFSMVGEILDDERDWTRCAKNQELPNVQVQTR 522

BLAST of CmaCh01G008300 vs. Swiss-Prot
Match: CDKAL_XENLA (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Xenopus laevis GN=cdkal1 PE=2 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 9.6e-139
Identity = 254/497 (51.11%), Postives = 337/497 (67.81%), Query Frame = 1

Query: 1   MEDIEDLLIGGGGGGGAPPGYRLPISAVGVKSKKKNKLKPNPDADDSSSGLFPTQNLLAP 60
           ++DIED++             R  I     K  K N  +  P AD +             
Sbjct: 9   LDDIEDMVSATDPKPHDRQSARKNIVPRARKRNKNNIQEEEPPADST------------- 68

Query: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSA 120
            IPGTQ I+++T+GCSHN SD EYMAGQL+A+GY ++E PE+ADLWL+N+CTVKSP++  
Sbjct: 69  -IPGTQKIWIRTWGCSHNNSDGEYMAGQLAAYGYSITEQPEKADLWLLNSCTVKSPAEDH 128

Query: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180
               I K + A K +VV+GCVPQ       ++G+SI+GVQQIDRVVEVVEET+KGH VRL
Sbjct: 129 FRNSIKKAQEANKKVVVSGCVPQAQPRQDYMKGLSIIGVQQIDRVVEVVEETIKGHSVRL 188

Query: 181 LNRKTLPA-------LDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSL 240
           L +K           LDLPK+RKN  IEI+ IN GCL ACTYCKTKHARG L SY V+ L
Sbjct: 189 LGQKKDNGKRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGELASYPVEEL 248

Query: 241 VNRVRTVINEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPP 300
           V+R      EGV EIWL+SEDTGAYGRDIG +LP LL  +V  +P  A  MLR+GMTNPP
Sbjct: 249 VDRATQSFQEGVCEIWLTSEDTGAYGRDIGTDLPTLLWKLVEVIPEGA--MLRLGMTNPP 308

Query: 301 FILEHLKEIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGM 360
           +ILEHL+E+A +L HP VY+FLH+PVQS SD++L  M REY +++F+ VVD L E VPG+
Sbjct: 309 YILEHLEEMAKILNHPRVYAFLHIPVQSASDSVLMDMKREYCIADFKRVVDFLKERVPGI 368

Query: 361 QVATDIICGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKR 420
            +ATDIICGFPGETDEDF ET+ L++EYK P + I+QFYPRPGTPAA+M++V + + K+R
Sbjct: 369 TIATDIICGFPGETDEDFKETLKLVEEYKFPSLFINQFYPRPGTPAAKMEQVLAHVKKRR 428

Query: 421 SRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHEAMLGTSAL 480
           ++EL+ +F ++ PY+   G+ +++ +TE + D  + V H + Y QVLV      +G    
Sbjct: 429 TKELSQLFHSYDPYDHKIGQKQQVLVTEESFDSQYYVAHNRFYEQVLVPKDPDFMGKMVE 488

Query: 481 VKITSVGRWSVFGEVIE 491
           VKI   G+  + G+ ++
Sbjct: 489 VKIFEAGKHFMKGQPVQ 489

BLAST of CmaCh01G008300 vs. Swiss-Prot
Match: CDKAL_HUMAN (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Homo sapiens GN=CDKAL1 PE=1 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 2.1e-138
Identity = 241/435 (55.40%), Postives = 318/435 (73.10%), Query Frame = 1

Query: 62  IPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSAM 121
           IPG Q I+++T+GCSHN SD EYMAGQL+A+GY ++EN  +ADLWL+N+CTVK+P++   
Sbjct: 60  IPGIQKIWIRTWGCSHNNSDGEYMAGQLAAYGYKITENASDADLWLLNSCTVKNPAEDHF 119

Query: 122 DTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLL 181
              I K +   K +V+AGCVPQ       L+G+SI+GVQQIDRVVEVVEET+KGH VRLL
Sbjct: 120 RNSIKKAQEENKKIVLAGCVPQAQPRQDYLKGLSIIGVQQIDRVVEVVEETIKGHSVRLL 179

Query: 182 NRKTLPA-------LDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV 241
            +K           LDLPK+RKN  IEI+ IN GCL ACTYCKTKHARG+L SY +D LV
Sbjct: 180 GQKKDNGRRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGNLASYPIDELV 239

Query: 242 NRVRTVINEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPF 301
           +R +    EGV EIWL+SEDTGAYGRDIG NLP LL  +V  +P  A  MLR+GMTNPP+
Sbjct: 240 DRAKQSFQEGVCEIWLTSEDTGAYGRDIGTNLPTLLWKLVEVIPEGA--MLRLGMTNPPY 299

Query: 302 ILEHLKEIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQ 361
           ILEHL+E+A +L HP VY+FLH+PVQS SD++L  M REY V++F+ VVD L E VPG+ 
Sbjct: 300 ILEHLEEMAKILNHPRVYAFLHIPVQSASDSVLMEMKREYCVADFKRVVDFLKEKVPGIT 359

Query: 362 VATDIICGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRS 421
           +ATDIICGFPGETD+DF ET+ L++EYK P + I+QFYPRPGTPAA+M++VP+ + K+R+
Sbjct: 360 IATDIICGFPGETDQDFQETVKLVEEYKFPSLFINQFYPRPGTPAAKMEQVPAQVKKQRT 419

Query: 422 RELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHEAMLGTSALV 481
           ++L+ VF +++PY+   G  +++ +TE + D    V H + Y QVLV  + A +G    V
Sbjct: 420 KDLSRVFHSYSPYDHKIGERQQVLVTEESFDSKFYVAHNQFYEQVLVPKNPAFMGKMVEV 479

Query: 482 KITSVGRWSVFGEVI 490
            I   G+  + G+ +
Sbjct: 480 DIYESGKHFMKGQPV 492

BLAST of CmaCh01G008300 vs. TrEMBL
Match: A0A0A0KSZ4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G652200 PE=4 SV=1)

HSP 1 Score: 1116.3 bits (2886), Expect = 0.0e+00
Identity = 565/637 (88.70%), Postives = 588/637 (92.31%), Query Frame = 1

Query: 1   MEDIEDLLIGGGGGGGAPPGYRLPISAVGVKSKKKNKLKPNPDADDSSSGLFPTQNLLAP 60
           MEDIEDLLIGGGGG  APPGYRLPI+AVGVK KKKN  K N  ADDSS+   P QN L P
Sbjct: 1   MEDIEDLLIGGGGG--APPGYRLPITAVGVKPKKKNMFKSNASADDSSNEPSPIQNPLVP 60

Query: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSA 120
           KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLS+NPE+ADLWLINTCTVKSPSQSA
Sbjct: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSA 120

Query: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180
           MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL
Sbjct: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180

Query: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTV 240
           LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVR+V
Sbjct: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVRRVRSV 240

Query: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK 300
           INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIV+ELP DASTMLRIGMTNPPFILEHLK
Sbjct: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVSELPSDASTMLRIGMTNPPFILEHLK 300

Query: 301 EIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDII 360
           EIA VL HPCVYSFLHVPVQSGSD ILSAMNREYTVSEFRTVVDTLTELVPGMQ+ATDII
Sbjct: 301 EIAKVLSHPCVYSFLHVPVQSGSDAILSAMNREYTVSEFRTVVDTLTELVPGMQIATDII 360

Query: 361 CGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420
           CGFPGETDEDFCETINLIKEY LPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV
Sbjct: 361 CGFPGETDEDFCETINLIKEYNLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420

Query: 421 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHEAMLGTSALVKITSVG 480
           FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVI  E MLGTSA VKITS+G
Sbjct: 421 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSATVKITSIG 480

Query: 481 RWSVFGEVIETIVTKHHKTTALEDMPIQAKVPPCSNSHETCACSVEPEACACGPESCCKG 540
           RWSVFGEVIE I TKHHKTT LED   Q KV PCSN+HETCACS EPE+CACG ES CKG
Sbjct: 481 RWSVFGEVIEIISTKHHKTTTLEDTLTQDKVSPCSNTHETCACSTEPESCACGLES-CKG 540

Query: 541 PVALDEKDDSSRNIPLPEEPKRKNLVEWLLRKRKSHV-PKREEGESPVVSGRKQASAGGS 600
            VA+ ++ +SSRN+P PEEPKRKNL+EW+LR+RKSHV PKREE E+P+V+ RKQ  AGG 
Sbjct: 541 AVAVGDEVNSSRNVPSPEEPKRKNLIEWVLRRRKSHVLPKREETENPIVTERKQTLAGGR 600

Query: 601 MSEWGVVDRILVGGILISIFTIVGLLFHLGSTTLSSS 637
           + EWGVVD+ILVGGILIS FTI GLLFHLGSTT SSS
Sbjct: 601 LDEWGVVDKILVGGILISTFTIFGLLFHLGSTTFSSS 634

BLAST of CmaCh01G008300 vs. TrEMBL
Match: B9S747_RICCO (Radical sam protein, putative OS=Ricinus communis GN=RCOM_1333590 PE=4 SV=1)

HSP 1 Score: 951.8 bits (2459), Expect = 4.2e-274
Identity = 486/638 (76.18%), Postives = 539/638 (84.48%), Query Frame = 1

Query: 1   MEDIEDLLIGGGGGGGAPPGYRLPISAVGVKSKK---KNKLKPNPDADDSSSGLFPTQNL 60
           MEDIEDLLIG G GGGAPPG+RLP+++VGV  KK   KNK   N        G+  +Q+L
Sbjct: 1   MEDIEDLLIGSGSGGGAPPGFRLPLNSVGVYPKKIIKKNKGYLN-------HGISLSQSL 60

Query: 61  LAPKIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPS 120
           L+PKIPGTQTIY+KTFGCSHNQSDSEYMAGQLS+FGY L++ PE+ DLWLINTCTVKSPS
Sbjct: 61  LSPKIPGTQTIYIKTFGCSHNQSDSEYMAGQLSSFGYALTDIPEDGDLWLINTCTVKSPS 120

Query: 121 QSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE 180
           QSAMDT+I K K+AKKPLVVAGCVPQGSR+LKELEGVSIVGVQQIDRVVEVVEETLKGHE
Sbjct: 121 QSAMDTIIAKGKSAKKPLVVAGCVPQGSRNLKELEGVSIVGVQQIDRVVEVVEETLKGHE 180

Query: 181 VRLLNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRV 240
           VRLL RKTLPALDLPKVRKNKF+EILPINVGCLGACTYCKTKHARGHLGSYT+DSLV RV
Sbjct: 181 VRLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTIDSLVGRV 240

Query: 241 RTVINEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILE 300
           RTV+ +GV+EIWLSSEDTGAYGRDIGVNLP LLNAIV+ELP DASTMLRIGMTNPPFILE
Sbjct: 241 RTVVGDGVKEIWLSSEDTGAYGRDIGVNLPRLLNAIVSELPADASTMLRIGMTNPPFILE 300

Query: 301 HLKEIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVAT 360
           HLKEIA VL HPCVYSFLHVPVQSGSD +L+AMNREYTVS FRTVVDTLTELVPGMQ+AT
Sbjct: 301 HLKEIAEVLRHPCVYSFLHVPVQSGSDNVLNAMNREYTVSNFRTVVDTLTELVPGMQIAT 360

Query: 361 DIICGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSREL 420
           DIICGFPGETD+DF +T++LI EYKLPQVHISQFYPRPGTPAARMKKVPS IVKKRSREL
Sbjct: 361 DIICGFPGETDDDFAQTVSLINEYKLPQVHISQFYPRPGTPAARMKKVPSNIVKKRSREL 420

Query: 421 TSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHEAMLGTSALVKIT 480
           T+VFEAFTPYNGMEGRVERIWITEIA DGIHLVGHTKGY+QVLVI  E MLGTSA+VKIT
Sbjct: 421 TAVFEAFTPYNGMEGRVERIWITEIATDGIHLVGHTKGYVQVLVIAPETMLGTSAIVKIT 480

Query: 481 SVGRWSVFGEVIETIVTKHHKTTALEDMPIQAKVPPCSNSHETCACSVEPEACACGPESC 540
           SVGRWSVFGEVI+T+   +    + E MP   K  PCS+  ETCACS EPE+CACGPESC
Sbjct: 481 SVGRWSVFGEVIQTLNQTNRGVASAEKMPSGGKYSPCSDPCETCACSKEPESCACGPESC 540

Query: 541 CKGPVALDEKDDSSRNIPLPEEPKRKNLVEWLLRKRKSHVPKREEGESPVVSGRKQASAG 600
             G     E+   ++N  L E+  R+NL+ WLLRKRK+   K  E    + S +KQ    
Sbjct: 541 --GGQNPLEESAIAQNDMLLEDRNRRNLIGWLLRKRKNQTQKIVENNIALESIKKQEWTK 600

Query: 601 GSMSEWGVVDRILVGGILISIFTIVGLLFHLGSTTLSS 636
           G+ S WG VDR L+GG+L+S+ TIV LL HLG  T S+
Sbjct: 601 GTFSMWGAVDRALLGGMLVSLVTIVALLLHLGFRTTST 629

BLAST of CmaCh01G008300 vs. TrEMBL
Match: A0A067KJB8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_10950 PE=4 SV=1)

HSP 1 Score: 943.0 bits (2436), Expect = 1.9e-271
Identity = 485/636 (76.26%), Postives = 542/636 (85.22%), Query Frame = 1

Query: 1   MEDIEDLLIGGGGGGGAPPGYRLPISAVGVKSKKKNKLKPNPDADDSSSGLFPTQNLLAP 60
           MEDIEDLLIGGGG G APPG+RLP++ VGV + +KNK KPN +    S   F      +P
Sbjct: 1   MEDIEDLLIGGGGIG-APPGFRLPLNTVGV-NPRKNKNKPNLNTTMLSQIPF------SP 60

Query: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSA 120
           KIPGTQTIY+KTFGCSHNQSDSEYMAGQLS+FGY L++ PE+ADLWLINTCTVKSPSQSA
Sbjct: 61  KIPGTQTIYIKTFGCSHNQSDSEYMAGQLSSFGYALTDTPEDADLWLINTCTVKSPSQSA 120

Query: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180
           MDTL+ K KNAKKPLVVAGCVPQGSR+LKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL
Sbjct: 121 MDTLLAKGKNAKKPLVVAGCVPQGSRNLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180

Query: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTV 240
           LNRKTLPALDLPKVRKNKF+EILPINVGCLGACTYCKTKHARGHLGSY++DSLV R+RTV
Sbjct: 181 LNRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYSIDSLVGRIRTV 240

Query: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK 300
           I +GV+EIWLSSEDTGAYGRDIGVNLPILLNAIVAELP DASTMLRIGMTNPPFILEHLK
Sbjct: 241 IADGVKEIWLSSEDTGAYGRDIGVNLPILLNAIVAELPADASTMLRIGMTNPPFILEHLK 300

Query: 301 EIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDII 360
           EIA VL HPCVYSFLHVPVQSGSD +LSAMNREYTVSEFRTVVDTL+ELVPGMQ+ATDII
Sbjct: 301 EIAEVLRHPCVYSFLHVPVQSGSDNVLSAMNREYTVSEFRTVVDTLSELVPGMQIATDII 360

Query: 361 CGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420
           CGFPGETDEDF +T++LI EYK PQVHISQFYPRPGTPAARMKKVPS +VKKRSRELTSV
Sbjct: 361 CGFPGETDEDFAQTVSLIDEYKFPQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELTSV 420

Query: 421 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHEAMLGTSALVKITSVG 480
           FEAFTPY+GMEG VERIWI +IA DGIHLVGHTKGY+QVLV   E+MLGTSA+VKITSVG
Sbjct: 421 FEAFTPYDGMEGTVERIWINDIATDGIHLVGHTKGYVQVLVAAPESMLGTSAIVKITSVG 480

Query: 481 RWSVFGEVI-ETIVTKHHKTTALEDMPIQAKVPPCSNSHETCACSVEPEACACGPESCCK 540
           RWSVFGEVI +T+     KT + + +P + K  PCS+  ETC CS EPE CACGPES C 
Sbjct: 481 RWSVFGEVIHQTLDQMVGKTVSDKMLPGEDKCSPCSDPCETCTCSKEPEPCACGPES-CG 540

Query: 541 GPVALDEKDDSSRNIPLPEEPKRKNLVEWLLRKRKSHVPKREEGESPVVSGRKQASAGGS 600
           G  AL+E   +  +I L ++  R+NL+ WLLR+RKS V K+ + E  + S +KQ  A GS
Sbjct: 541 GQKALEESATAQNDI-LLDDRNRRNLIGWLLRRRKSKVHKKIDNEIALGSMKKQECARGS 600

Query: 601 MSEWGVVDRILVGGILISIFTIVGLLFHLGSTTLSS 636
           +S W  VDR L+GG+L+S+ TI  LL H G  T+SS
Sbjct: 601 LSMWSPVDRALIGGVLVSLLTIAALLMHFGYRTISS 626

BLAST of CmaCh01G008300 vs. TrEMBL
Match: A0A061FK81_THECC (Methylthiotransferase OS=Theobroma cacao GN=TCM_036466 PE=4 SV=1)

HSP 1 Score: 937.6 bits (2422), Expect = 8.1e-270
Identity = 483/635 (76.06%), Postives = 536/635 (84.41%), Query Frame = 1

Query: 1   MEDIEDLLIGGGGGGGAPPGYRLPISAVGVKSKKKNKLKPNPDADDSSSGLFPTQNLLAP 60
           MEDIEDLL+G GG   APPG+RLP++AVGV  +KK++ KPN  +  +     P   LL P
Sbjct: 1   MEDIEDLLVGSGG---APPGFRLPLNAVGVNPRKKHRNKPNKISQTND----PLTPLLPP 60

Query: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSA 120
           KIPGTQTIY+KTFGCSHNQSDSEYMAGQLSAFGY +S+NPEE DLWLINTCTVKSPSQSA
Sbjct: 61  KIPGTQTIYIKTFGCSHNQSDSEYMAGQLSAFGYAISDNPEEGDLWLINTCTVKSPSQSA 120

Query: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180
           M+TLITKCK+AKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL
Sbjct: 121 MNTLITKCKSAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180

Query: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTV 240
           +NRKTLP LDLPKVRKNKF+EILPINVGCLGACTYCKTKHARGHLGSYT+DSLV RV+TV
Sbjct: 181 MNRKTLPGLDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTIDSLVERVKTV 240

Query: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK 300
           I +GV+EIWLSSEDTGAYGRDIGVNLPILLNA+VAELPPD +TMLRIGMTNPPFILEHLK
Sbjct: 241 IADGVKEIWLSSEDTGAYGRDIGVNLPILLNALVAELPPDGNTMLRIGMTNPPFILEHLK 300

Query: 301 EIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDII 360
           EIA+VL HPCVYSFLHVPVQSGSD IL+AMNREYTVSEFRTVVDTLTELVPGMQ+ATDII
Sbjct: 301 EIADVLRHPCVYSFLHVPVQSGSDAILTAMNREYTVSEFRTVVDTLTELVPGMQIATDII 360

Query: 361 CGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420
           CGFPGETDEDF +T+NLIKEYK PQVHISQFYPRPGTPAARMKKVPS IVK RSRELTSV
Sbjct: 361 CGFPGETDEDFAQTVNLIKEYKFPQVHISQFYPRPGTPAARMKKVPSTIVKNRSRELTSV 420

Query: 421 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHEAMLGTSALVKITSVG 480
           FEAFTPY GMEGRVERIWITE+AADGIHLVGHTKGY+QVLVIG E+MLGTSA+VKITSVG
Sbjct: 421 FEAFTPYIGMEGRVERIWITEVAADGIHLVGHTKGYVQVLVIGPESMLGTSAIVKITSVG 480

Query: 481 RWSVFGEVIETIVTKHHKTTALEDMPIQAKVPPCSNSHETCACSVEPEACACGPESCCKG 540
           RWSVFGEVIET+ T+ +   A E +  Q K     N  ++ ACS EPE C+CGP SC  G
Sbjct: 481 RWSVFGEVIETL-TQVNLKMAPEKVCNQEKCSTRFNQFDSGACSKEPEPCSCGPNSC--G 540

Query: 541 PVALDEKDDSSRNIPLPEEPKRKNLVEWLLRKRKSHVPKREEGESPVVSGRKQASAGGSM 600
              + E+   S N    E+   KNL+ WLLRKR++HV K+ E    +   +KQ  A G  
Sbjct: 541 GQTILEECTVSSNDSWMEDRSSKNLIGWLLRKRRNHVHKKVENGLALGLRKKQDWALGG- 600

Query: 601 SEWGVVDRILVGGILISIFTIVGLLFHLGSTTLSS 636
             WG+VDR L+ GIL+S F IV ++ HL   T+ S
Sbjct: 601 --WGIVDRALLVGILVSFFIIVAVIIHLEFRTMLS 622

BLAST of CmaCh01G008300 vs. TrEMBL
Match: W9RWT6_9ROSA (CDK5 regulatory subunit-associated protein 1-like 1 OS=Morus notabilis GN=L484_025461 PE=4 SV=1)

HSP 1 Score: 937.6 bits (2422), Expect = 8.1e-270
Identity = 484/635 (76.22%), Postives = 530/635 (83.46%), Query Frame = 1

Query: 1   MEDIEDLLIGGGGGGGAPPGYRLPISAVGVKSKKKNKLKPNPDADDSSSGLFPTQNLLAP 60
           MEDIEDLL+G  GG  APPG+RLP++AVG+K KK  K KP          L    N LAP
Sbjct: 1   MEDIEDLLVGSTGG--APPGFRLPVTAVGLKPKK-TKNKP----------LLNKNNTLAP 60

Query: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSA 120
            IPGTQTIY+KTFGCSHNQSDSEYMAGQLSAFGY LS+NP+EADLWLINTCTVKSPSQSA
Sbjct: 61  NIPGTQTIYIKTFGCSHNQSDSEYMAGQLSAFGYSLSDNPDEADLWLINTCTVKSPSQSA 120

Query: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180
           MDTLITK KNAKKPLVVAGCVPQGSRDLKELEGVS+VGVQQIDRVVEVVEETLKGHEVRL
Sbjct: 121 MDTLITKGKNAKKPLVVAGCVPQGSRDLKELEGVSVVGVQQIDRVVEVVEETLKGHEVRL 180

Query: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTV 240
           L RKTLPALDLPKVRKN+F+EILPINVGCLGACTYCKTKHARGHLGSY VDSLV RVRTV
Sbjct: 181 LTRKTLPALDLPKVRKNEFVEILPINVGCLGACTYCKTKHARGHLGSYIVDSLVGRVRTV 240

Query: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK 300
           I +GV+EIWLSSEDTGAYGRDIGVNLPILL AIVAELPPDASTMLRIGMTNPPFILEHLK
Sbjct: 241 IADGVKEIWLSSEDTGAYGRDIGVNLPILLKAIVAELPPDASTMLRIGMTNPPFILEHLK 300

Query: 301 EIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDII 360
           EIA+VL HPCVYSFLHVPVQSGSD +L+AMNREYTVSEFRTVVDTLTELVPGMQ+ATDII
Sbjct: 301 EIADVLHHPCVYSFLHVPVQSGSDAVLTAMNREYTVSEFRTVVDTLTELVPGMQIATDII 360

Query: 361 CGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420
           CGFPGETDEDF +TI LI +YK PQVHISQFYPRPGTPAA+MKKVPS IVKKRSRELTSV
Sbjct: 361 CGFPGETDEDFAQTIGLINKYKFPQVHISQFYPRPGTPAAKMKKVPSTIVKKRSRELTSV 420

Query: 421 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHEAMLGTSALVKITSVG 480
           FEAFTPYNGMEGRVERIWIT+IA DG+HLVGHTKGY+QVLV+  E+MLGTSA+VKITSVG
Sbjct: 421 FEAFTPYNGMEGRVERIWITDIATDGVHLVGHTKGYVQVLVVAPESMLGTSAIVKITSVG 480

Query: 481 RWSVFGEVIETIVTKHHKTTALEDMPIQAKVPPCSNSHETCACSVEPEACACGPESCCKG 540
           RWSVFGEVIETI   ++K T+      + K  PCS+    CA S   E CACGPE C  G
Sbjct: 481 RWSVFGEVIETIQDVNYKKTSSTRSSSENKCSPCSDPCNACASSRVQETCACGPEGC--G 540

Query: 541 PVALDEKDDSSRNIPLPEEPKRKNLVEWLLRKRKSHVPKREEGESPVVSGRKQASAGGSM 600
              L+E   S+  IP  E+   +NL+ WLLRKRK++V ++ E E      +K    G S 
Sbjct: 541 GTTLEESAVSTNAIP-QEDRSSRNLIGWLLRKRKNNVVQKMESEVSSGFKKKLEWVGESK 600

Query: 601 SEWGVVDRILVGGILISIFTIVGLLFHLGSTTLSS 636
             W VVDR+L+GGIL+S  TI+ LL H G  TLS+
Sbjct: 601 GSWNVVDRVLLGGILVSFLTILTLLIHHGFRTLSN 619

BLAST of CmaCh01G008300 vs. TAIR10
Match: AT1G72090.1 (AT1G72090.1 Methylthiotransferase)

HSP 1 Score: 846.3 bits (2185), Expect = 1.2e-245
Identity = 447/638 (70.06%), Postives = 505/638 (79.15%), Query Frame = 1

Query: 1   MEDIEDLLIGGGGGGGAPPGYRLPISAVGV--KSKKKNKLKPNPDADDSSSGLFPTQNLL 60
           MEDIEDLL   GG GGAPPG+RLP++AVG+  K+ K  ++   PD   +S+     ++ L
Sbjct: 1   MEDIEDLL--AGGVGGAPPGFRLPLNAVGINPKTNKSKRISSKPDQITASN-----RDSL 60

Query: 61  AP---KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKS 120
           AP   KIPGTQTIY+KTFGCSHNQSDSEYMAGQLSAFGY L+E PEEADLWLINTCTVKS
Sbjct: 61  APPSMKIPGTQTIYIKTFGCSHNQSDSEYMAGQLSAFGYALTEVPEEADLWLINTCTVKS 120

Query: 121 PSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKG 180
           PSQSAM TLIT+ ++ KKPLV+AGCVPQGSRDLKELEGVS+VGVQQIDRVVE+VEETLKG
Sbjct: 121 PSQSAMSTLITRGRSGKKPLVIAGCVPQGSRDLKELEGVSVVGVQQIDRVVEIVEETLKG 180

Query: 181 HEVRLLNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVN 240
           HEVRLL RKTLPALDLPKVR+N FIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV 
Sbjct: 181 HEVRLLTRKTLPALDLPKVRRNNFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVE 240

Query: 241 RVRTVINEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFI 300
           RVRTVI+EGV+EIWLSSEDTGAYGRDIGVNLPILLNAIV ELP D STMLRIGMTNPPFI
Sbjct: 241 RVRTVISEGVKEIWLSSEDTGAYGRDIGVNLPILLNAIVKELPSDQSTMLRIGMTNPPFI 300

Query: 301 LEHLKEIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQV 360
           LEHLKEIA VL HPCVY+FLHVPVQSGSD++L+AMNREYT SEFRTVVDTLTELVPGMQ+
Sbjct: 301 LEHLKEIAAVLRHPCVYTFLHVPVQSGSDSVLTAMNREYTASEFRTVVDTLTELVPGMQI 360

Query: 361 ATDIICGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSR 420
           ATDIICGFPGETDEDF +T+ LIK+YK PQVHISQFYPRPGTPAA+MKKV S IVK+RSR
Sbjct: 361 ATDIICGFPGETDEDFSQTVELIKDYKFPQVHISQFYPRPGTPAAKMKKVQSKIVKQRSR 420

Query: 421 ELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHEAMLGTSALVK 480
           ELTSVFEAF PY GME R ERIWITE+A DGIHLVGHTKGY+QVLV G E+MLGTSA+ +
Sbjct: 421 ELTSVFEAFAPYTGMECREERIWITEVATDGIHLVGHTKGYVQVLVTGPESMLGTSAMAR 480

Query: 481 ITSVGRWSVFGEVIETIVTKHHKTTALEDMPIQAKVPPCSNSHETCACSVEPEACACGPE 540
           ITSVGRWSVFGEVIET  + + +T + E+  +     PCS++  TC      E C C  E
Sbjct: 481 ITSVGRWSVFGEVIETFSSANRETKSREETKL-----PCSSNVSTC------ETCTCSAE 540

Query: 541 SCCKGPVALDEKDDSSRNIPLPEEPKRKNLVEWLLRKRKSHVPKREEGESPVVSGRKQAS 600
           SC  G     E  + S NI   ++ K K+              K+EE E   V       
Sbjct: 541 SC--GEERSGEACNISGNISGQDDNKGKS--------------KKEEKEVQEV-----VV 599

Query: 601 AGGSMSEWGVVDRILVGGILISIFTIVGLLFHLGSTTL 634
            G S++ WG +D+ LV G+ +S  TI+ LL  +    L
Sbjct: 601 PGSSVANWGFIDKALVCGVFVSSVTILVLLISIACRVL 599

BLAST of CmaCh01G008300 vs. TAIR10
Match: AT4G36390.1 (AT4G36390.1 Methylthiotransferase)

HSP 1 Score: 105.9 bits (263), Expect = 9.2e-23
Identity = 94/399 (23.56%), Postives = 169/399 (42.36%), Query Frame = 1

Query: 66  QTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSAMDTLI 125
           + I+V T  C+  ++  + +  +L+ F +L  E       W +N  T ++          
Sbjct: 168 EVIFVNT--CAIRENAEQRVWQRLNYFWFLKRE-------WKVNAATGRA---------- 227

Query: 126 TKCKNAKKP-LVVAGCVPQGSRDLKELEGVSIVGV-------QQIDRVVEVVEETLKGHE 185
              K+ K P +VV GC+ +  +D K L+   +V V       + + R++E V+   KG  
Sbjct: 228 ---KSLKPPKVVVLGCMAERLKD-KILDSDKMVDVVCGPDAYRDLPRLLEEVDYGQKGIN 287

Query: 186 VRLLNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRV 245
             L   +T   +   ++ +N     + +  GC   C +C     RG   S  V+S++  V
Sbjct: 288 TLLSLEETYADISPVRISENSITAFVSVMRGCNNMCAFCIVPFTRGRERSRPVESIIREV 347

Query: 246 RTVINEGVREIWLSSEDTGAYGRD------------------------IGVNLPILLNAI 305
             +   GV+E+ L  ++  +Y  D                        +G+    LL+ +
Sbjct: 348 GELWESGVKEVTLLGQNVNSYNDDSADRESGANWEYSEGFSSRCKVKNMGLRFADLLDRL 407

Query: 306 VAELPPDASTMLRIGMTNP-----PFILEHLKEIANVLCHPCVYSFLHVPVQSGSDTILS 365
             E P      +R   T+P     P  L +L    + +C+      +H+P QSG+  IL 
Sbjct: 408 SVEFPE-----MRFRFTSPHPKDYPDELLYLMRDRHNICN-----LIHLPAQSGNSRILE 467

Query: 366 AMNREYTVSEFRTVVDTLTELVPGMQVATDIICGFPGETDEDFCETINLIKEYKLPQVHI 425
            M R YT   +  +V  +  ++P + + +D I GF GET+E+  ET++L++       ++
Sbjct: 468 QMRRGYTREAYLDLVKKIRSIIPDVAITSDFITGFCGETEEEHQETLSLVRAVGYDMAYM 527

BLAST of CmaCh01G008300 vs. NCBI nr
Match: gi|449447617|ref|XP_004141564.1| (PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Cucumis sativus])

HSP 1 Score: 1116.3 bits (2886), Expect = 0.0e+00
Identity = 565/637 (88.70%), Postives = 588/637 (92.31%), Query Frame = 1

Query: 1   MEDIEDLLIGGGGGGGAPPGYRLPISAVGVKSKKKNKLKPNPDADDSSSGLFPTQNLLAP 60
           MEDIEDLLIGGGGG  APPGYRLPI+AVGVK KKKN  K N  ADDSS+   P QN L P
Sbjct: 1   MEDIEDLLIGGGGG--APPGYRLPITAVGVKPKKKNMFKSNASADDSSNEPSPIQNPLVP 60

Query: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSA 120
           KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLS+NPE+ADLWLINTCTVKSPSQSA
Sbjct: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSA 120

Query: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180
           MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL
Sbjct: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180

Query: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTV 240
           LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVR+V
Sbjct: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVRRVRSV 240

Query: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK 300
           INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIV+ELP DASTMLRIGMTNPPFILEHLK
Sbjct: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVSELPSDASTMLRIGMTNPPFILEHLK 300

Query: 301 EIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDII 360
           EIA VL HPCVYSFLHVPVQSGSD ILSAMNREYTVSEFRTVVDTLTELVPGMQ+ATDII
Sbjct: 301 EIAKVLSHPCVYSFLHVPVQSGSDAILSAMNREYTVSEFRTVVDTLTELVPGMQIATDII 360

Query: 361 CGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420
           CGFPGETDEDFCETINLIKEY LPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV
Sbjct: 361 CGFPGETDEDFCETINLIKEYNLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420

Query: 421 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHEAMLGTSALVKITSVG 480
           FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVI  E MLGTSA VKITS+G
Sbjct: 421 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSATVKITSIG 480

Query: 481 RWSVFGEVIETIVTKHHKTTALEDMPIQAKVPPCSNSHETCACSVEPEACACGPESCCKG 540
           RWSVFGEVIE I TKHHKTT LED   Q KV PCSN+HETCACS EPE+CACG ES CKG
Sbjct: 481 RWSVFGEVIEIISTKHHKTTTLEDTLTQDKVSPCSNTHETCACSTEPESCACGLES-CKG 540

Query: 541 PVALDEKDDSSRNIPLPEEPKRKNLVEWLLRKRKSHV-PKREEGESPVVSGRKQASAGGS 600
            VA+ ++ +SSRN+P PEEPKRKNL+EW+LR+RKSHV PKREE E+P+V+ RKQ  AGG 
Sbjct: 541 AVAVGDEVNSSRNVPSPEEPKRKNLIEWVLRRRKSHVLPKREETENPIVTERKQTLAGGR 600

Query: 601 MSEWGVVDRILVGGILISIFTIVGLLFHLGSTTLSSS 637
           + EWGVVD+ILVGGILIS FTI GLLFHLGSTT SSS
Sbjct: 601 LDEWGVVDKILVGGILISTFTIFGLLFHLGSTTFSSS 634

BLAST of CmaCh01G008300 vs. NCBI nr
Match: gi|659119467|ref|XP_008459673.1| (PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Cucumis melo])

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 566/637 (88.85%), Postives = 589/637 (92.46%), Query Frame = 1

Query: 1   MEDIEDLLIGGGGGGGAPPGYRLPISAVGVKSKKKNKLKPNPDADDSSSGLFPTQNLLAP 60
           MEDIEDLLIGGGG   APPGYRLPI+AVGVK KKKN  K NP ADDSS+   PTQN L P
Sbjct: 1   MEDIEDLLIGGGG---APPGYRLPITAVGVKPKKKNLFKSNPSADDSSNQPSPTQNPLVP 60

Query: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSA 120
           KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLS+NPE+ADLWLINTCTVKSPSQSA
Sbjct: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSA 120

Query: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180
           MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL
Sbjct: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180

Query: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTV 240
           LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVR+V
Sbjct: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRSV 240

Query: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK 300
           INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK
Sbjct: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK 300

Query: 301 EIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDII 360
           EIA VL HPCVYSFLHVPVQSGSD ILSAMNREYTVSEFRTVVDTLTELVPGMQ+ATDII
Sbjct: 301 EIAKVLSHPCVYSFLHVPVQSGSDAILSAMNREYTVSEFRTVVDTLTELVPGMQIATDII 360

Query: 361 CGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420
           CGFPGETDEDFCETINLIKEY LPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV
Sbjct: 361 CGFPGETDEDFCETINLIKEYNLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420

Query: 421 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHEAMLGTSALVKITSVG 480
           FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVI  E MLGTSA+ KITS+G
Sbjct: 421 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMAKITSIG 480

Query: 481 RWSVFGEVIETIVTKHHKTTALEDMPIQAKVPPCSNSHETCACSVEPEACACGPESCCKG 540
           RWSVFGEVIETI TKHHKTT LED   + KV PCSN+HETCACS EPE+CAC   S CKG
Sbjct: 481 RWSVFGEVIETISTKHHKTT-LEDTLTRDKVSPCSNTHETCACSTEPESCACELGS-CKG 540

Query: 541 PVALDEKDDSSRNIPLPEEPKRKNLVEWLLRKRKSHV-PKREEGESPVVSGRKQASAGGS 600
            V++ ++ DSSRNIPLPEEPKRKNL+EW+LR+RKS V PKREE E+P+V+ RKQ  AGG 
Sbjct: 541 AVSVGDEVDSSRNIPLPEEPKRKNLIEWVLRRRKSQVMPKREETENPIVTERKQTLAGGR 600

Query: 601 MSEWGVVDRILVGGILISIFTIVGLLFHLGSTTLSSS 637
           + EWGVVD ILVGGILIS FTI GLLFH+GSTTLSSS
Sbjct: 601 LDEWGVVDMILVGGILISTFTIFGLLFHVGSTTLSSS 632

BLAST of CmaCh01G008300 vs. NCBI nr
Match: gi|659119469|ref|XP_008459674.1| (PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2 [Cucumis melo])

HSP 1 Score: 991.5 bits (2562), Expect = 6.8e-286
Identity = 503/564 (89.18%), Postives = 525/564 (93.09%), Query Frame = 1

Query: 74  GCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSAMDTLITKCKNAKK 133
           G   + SDSEYMAGQLSAFGYLLS+NPE+ADLWLINTCTVKSPSQSAMDTLITKCKNAKK
Sbjct: 15  GRKKSMSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDTLITKCKNAKK 74

Query: 134 PLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPK 193
           PLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPK
Sbjct: 75  PLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPK 134

Query: 194 VRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTVINEGVREIWLSSE 253
           VRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVR+VINEGVREIWLSSE
Sbjct: 135 VRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRSVINEGVREIWLSSE 194

Query: 254 DTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIANVLCHPCVYS 313
           DTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA VL HPCVYS
Sbjct: 195 DTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIAKVLSHPCVYS 254

Query: 314 FLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIICGFPGETDEDFCE 373
           FLHVPVQSGSD ILSAMNREYTVSEFRTVVDTLTELVPGMQ+ATDIICGFPGETDEDFCE
Sbjct: 255 FLHVPVQSGSDAILSAMNREYTVSEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCE 314

Query: 374 TINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGR 433
           TINLIKEY LPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGR
Sbjct: 315 TINLIKEYNLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGR 374

Query: 434 VERIWITEIAADGIHLVGHTKGYIQVLVIGHEAMLGTSALVKITSVGRWSVFGEVIETIV 493
           VERIWITEIAADGIHLVGHTKGYIQVLVI  E MLGTSA+ KITS+GRWSVFGEVIETI 
Sbjct: 375 VERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMAKITSIGRWSVFGEVIETIS 434

Query: 494 TKHHKTTALEDMPIQAKVPPCSNSHETCACSVEPEACACGPESCCKGPVALDEKDDSSRN 553
           TKHHKTT LED   + KV PCSN+HETCACS EPE+CAC   S CKG V++ ++ DSSRN
Sbjct: 435 TKHHKTT-LEDTLTRDKVSPCSNTHETCACSTEPESCACELGS-CKGAVSVGDEVDSSRN 494

Query: 554 IPLPEEPKRKNLVEWLLRKRKSHV-PKREEGESPVVSGRKQASAGGSMSEWGVVDRILVG 613
           IPLPEEPKRKNL+EW+LR+RKS V PKREE E+P+V+ RKQ  AGG + EWGVVD ILVG
Sbjct: 495 IPLPEEPKRKNLIEWVLRRRKSQVMPKREETENPIVTERKQTLAGGRLDEWGVVDMILVG 554

Query: 614 GILISIFTIVGLLFHLGSTTLSSS 637
           GILIS FTI GLLFH+GSTTLSSS
Sbjct: 555 GILISTFTIFGLLFHVGSTTLSSS 576

BLAST of CmaCh01G008300 vs. NCBI nr
Match: gi|778708092|ref|XP_011656120.1| (PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2 [Cucumis sativus])

HSP 1 Score: 986.5 bits (2549), Expect = 2.2e-284
Identity = 497/553 (89.87%), Postives = 518/553 (93.67%), Query Frame = 1

Query: 85  MAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQG 144
           MAGQLSAFGYLLS+NPE+ADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQG
Sbjct: 1   MAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQG 60

Query: 145 SRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKFIEILP 204
           SRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKFIEILP
Sbjct: 61  SRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKFIEILP 120

Query: 205 INVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTVINEGVREIWLSSEDTGAYGRDIGV 264
           INVGCLGACTYCKTKHARGHLGSYTVDSLV RVR+VINEGVREIWLSSEDTGAYGRDIGV
Sbjct: 121 INVGCLGACTYCKTKHARGHLGSYTVDSLVRRVRSVINEGVREIWLSSEDTGAYGRDIGV 180

Query: 265 NLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIANVLCHPCVYSFLHVPVQSGSD 324
           NLPILLNAIV+ELP DASTMLRIGMTNPPFILEHLKEIA VL HPCVYSFLHVPVQSGSD
Sbjct: 181 NLPILLNAIVSELPSDASTMLRIGMTNPPFILEHLKEIAKVLSHPCVYSFLHVPVQSGSD 240

Query: 325 TILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIICGFPGETDEDFCETINLIKEYKLP 384
            ILSAMNREYTVSEFRTVVDTLTELVPGMQ+ATDIICGFPGETDEDFCETINLIKEY LP
Sbjct: 241 AILSAMNREYTVSEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCETINLIKEYNLP 300

Query: 385 QVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAA 444
           QVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAA
Sbjct: 301 QVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAA 360

Query: 445 DGIHLVGHTKGYIQVLVIGHEAMLGTSALVKITSVGRWSVFGEVIETIVTKHHKTTALED 504
           DGIHLVGHTKGYIQVLVI  E MLGTSA VKITS+GRWSVFGEVIE I TKHHKTT LED
Sbjct: 361 DGIHLVGHTKGYIQVLVIAPETMLGTSATVKITSIGRWSVFGEVIEIISTKHHKTTTLED 420

Query: 505 MPIQAKVPPCSNSHETCACSVEPEACACGPESCCKGPVALDEKDDSSRNIPLPEEPKRKN 564
              Q KV PCSN+HETCACS EPE+CACG ES CKG VA+ ++ +SSRN+P PEEPKRKN
Sbjct: 421 TLTQDKVSPCSNTHETCACSTEPESCACGLES-CKGAVAVGDEVNSSRNVPSPEEPKRKN 480

Query: 565 LVEWLLRKRKSHV-PKREEGESPVVSGRKQASAGGSMSEWGVVDRILVGGILISIFTIVG 624
           L+EW+LR+RKSHV PKREE E+P+V+ RKQ  AGG + EWGVVD+ILVGGILIS FTI G
Sbjct: 481 LIEWVLRRRKSHVLPKREETENPIVTERKQTLAGGRLDEWGVVDKILVGGILISTFTIFG 540

Query: 625 LLFHLGSTTLSSS 637
           LLFHLGSTT SSS
Sbjct: 541 LLFHLGSTTFSSS 552

BLAST of CmaCh01G008300 vs. NCBI nr
Match: gi|659119471|ref|XP_008459676.1| (PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase isoform X3 [Cucumis melo])

HSP 1 Score: 980.3 bits (2533), Expect = 1.6e-282
Identity = 497/553 (89.87%), Postives = 518/553 (93.67%), Query Frame = 1

Query: 85  MAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQG 144
           MAGQLSAFGYLLS+NPE+ADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQG
Sbjct: 1   MAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQG 60

Query: 145 SRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKFIEILP 204
           SRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKFIEILP
Sbjct: 61  SRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKFIEILP 120

Query: 205 INVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTVINEGVREIWLSSEDTGAYGRDIGV 264
           INVGCLGACTYCKTKHARGHLGSYTVDSLV RVR+VINEGVREIWLSSEDTGAYGRDIGV
Sbjct: 121 INVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRSVINEGVREIWLSSEDTGAYGRDIGV 180

Query: 265 NLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIANVLCHPCVYSFLHVPVQSGSD 324
           NLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA VL HPCVYSFLHVPVQSGSD
Sbjct: 181 NLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIAKVLSHPCVYSFLHVPVQSGSD 240

Query: 325 TILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIICGFPGETDEDFCETINLIKEYKLP 384
            ILSAMNREYTVSEFRTVVDTLTELVPGMQ+ATDIICGFPGETDEDFCETINLIKEY LP
Sbjct: 241 AILSAMNREYTVSEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCETINLIKEYNLP 300

Query: 385 QVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAA 444
           QVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAA
Sbjct: 301 QVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAA 360

Query: 445 DGIHLVGHTKGYIQVLVIGHEAMLGTSALVKITSVGRWSVFGEVIETIVTKHHKTTALED 504
           DGIHLVGHTKGYIQVLVI  E MLGTSA+ KITS+GRWSVFGEVIETI TKHHKTT LED
Sbjct: 361 DGIHLVGHTKGYIQVLVIAPETMLGTSAMAKITSIGRWSVFGEVIETISTKHHKTT-LED 420

Query: 505 MPIQAKVPPCSNSHETCACSVEPEACACGPESCCKGPVALDEKDDSSRNIPLPEEPKRKN 564
              + KV PCSN+HETCACS EPE+CAC   S CKG V++ ++ DSSRNIPLPEEPKRKN
Sbjct: 421 TLTRDKVSPCSNTHETCACSTEPESCACELGS-CKGAVSVGDEVDSSRNIPLPEEPKRKN 480

Query: 565 LVEWLLRKRKSHV-PKREEGESPVVSGRKQASAGGSMSEWGVVDRILVGGILISIFTIVG 624
           L+EW+LR+RKS V PKREE E+P+V+ RKQ  AGG + EWGVVD ILVGGILIS FTI G
Sbjct: 481 LIEWVLRRRKSQVMPKREETENPIVTERKQTLAGGRLDEWGVVDMILVGGILISTFTIFG 540

Query: 625 LLFHLGSTTLSSS 637
           LLFH+GSTTLSSS
Sbjct: 541 LLFHVGSTTLSSS 551

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CDKAL_DROPS1.9e-14252.92Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Drosophila pseudoobscur... [more]
CDKAL_XENTR3.0e-14053.10Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Xenopus tropicalis GN=c... [more]
CDKAL_DROME2.5e-13950.67Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Drosophila melanogaster... [more]
CDKAL_XENLA9.6e-13951.11Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Xenopus laevis GN=cdkal... [more]
CDKAL_HUMAN2.1e-13855.40Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Homo sapiens GN=CDKAL1 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KSZ4_CUCSA0.0e+0088.70Uncharacterized protein OS=Cucumis sativus GN=Csa_5G652200 PE=4 SV=1[more]
B9S747_RICCO4.2e-27476.18Radical sam protein, putative OS=Ricinus communis GN=RCOM_1333590 PE=4 SV=1[more]
A0A067KJB8_JATCU1.9e-27176.26Uncharacterized protein OS=Jatropha curcas GN=JCGZ_10950 PE=4 SV=1[more]
A0A061FK81_THECC8.1e-27076.06Methylthiotransferase OS=Theobroma cacao GN=TCM_036466 PE=4 SV=1[more]
W9RWT6_9ROSA8.1e-27076.22CDK5 regulatory subunit-associated protein 1-like 1 OS=Morus notabilis GN=L484_0... [more]
Match NameE-valueIdentityDescription
AT1G72090.11.2e-24570.06 Methylthiotransferase[more]
AT4G36390.19.2e-2323.56 Methylthiotransferase[more]
Match NameE-valueIdentityDescription
gi|449447617|ref|XP_004141564.1|0.0e+0088.70PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Cuc... [more]
gi|659119467|ref|XP_008459673.1|0.0e+0088.85PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Cuc... [more]
gi|659119469|ref|XP_008459674.1|6.8e-28689.18PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2 [Cuc... [more]
gi|778708092|ref|XP_011656120.1|2.2e-28489.87PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2 [Cuc... [more]
gi|659119471|ref|XP_008459676.1|1.6e-28289.87PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase isoform X3 [Cuc... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002792TRAM_dom
IPR005839Methylthiotransferase
IPR006466MiaB-like_B
IPR006638Elp3/MiaB/NifB
IPR007197rSAM
IPR013848Methylthiotransferase_N
IPR020612Methylthiotransferase_CS
IPR023404rSAM_horseshoe
IPR023970Methylthiotransferase/radical SAM-type protein
Vocabulary: Molecular Function
TermDefinition
GO:00515394 iron, 4 sulfur cluster binding
GO:0016740transferase activity
GO:0003824catalytic activity
GO:0051536iron-sulfur cluster binding
Vocabulary: Biological Process
TermDefinition
GO:0043412macromolecule modification
GO:0009451RNA modification
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0009451 RNA modification
biological_process GO:0043412 macromolecule modification
biological_process GO:0006400 tRNA modification
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0032440 2-alkenal reductase [NAD(P)] activity
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0016740 transferase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0051536 iron-sulfur cluster binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G008300.1CmaCh01G008300.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002792TRAM domainPROFILEPS50926TRAMcoord: 428..490
score: 10
IPR005839MethylthiotransferaseTIGRFAMsTIGR00089TIGR00089coord: 67..484
score: 7.8
IPR006466MiaB-like tRNA modifying enzyme, archaeal-typeTIGRFAMsTIGR01578TIGR01578coord: 68..489
score: 1.3E
IPR006638Elongator protein 3/MiaB/NifBSMARTSM00729MiaBcoord: 199..420
score: 2.6
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 207..378
score: 2.4
IPR013848Methylthiotransferase, N-terminalPFAMPF00919UPF0004coord: 68..146
score: 8.3
IPR013848Methylthiotransferase, N-terminalPROFILEPS51449MTTASE_Ncoord: 66..174
score: 29
IPR020612Methylthiotransferase, conserved sitePROSITEPS01278MTTASE_RADICALcoord: 203..223
scor
IPR023404Radical SAM, alpha/beta horseshoeGENE3DG3DSA:3.80.30.20coord: 203..423
score: 1.3
IPR023970Methylthiotransferase/radical SAM-type proteinPANTHERPTHR11918RADICAL SAM PROTEINScoord: 1..538
score: 2.9E
NoneNo IPR availablePANTHERPTHR11918:SF45THREONYLCARBAMOYLADENOSINE TRNA METHYLTHIOTRANSFERASEcoord: 1..538
score: 2.9E
NoneNo IPR availableunknownSSF102114Radical SAM enzymescoord: 189..423
score: 3.14