Cla97C07G130810 (gene) Watermelon (97103) v2

NameCla97C07G130810
Typegene
OrganismCitrullus lanatus (Watermelon (97103) v2)
DescriptionSerine/threonine protein kinase putative
LocationCla97Chr07 : 2458170 .. 2462112 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGATTTTCTTCGCCGATTCTCAAATCTCCACCACCACAGCATCCGCTCAGGACCCCTTGTTGCCTTGGCTCTGGTATTCCTTGATTTCTTTTATGCGTTCGAGCTAATTGACAGCCGTTCTTACATTCAAACTCAGGAAAATTGTTGGCGCTATTTGGTTCCAATTGCAGGTCCATCAAAGATGCTCTTGAAAATTCAACTTCAGGCAATGTTTCTGGGCCAGAACTCGCCAACCTTCTCTCTGATTGCATTGAAAATTTCAGCGGCAATGTCAAGTACAAAAACGATGTCCGATTTCTCAAGATTTGGTTTCTCTCTGTGCGTAACTCTCTCTCTTTCACTGTTACATTTGTGGGTTCAACTTATCATCTTTGTTTCATACATACGGTTGATTAGATCACTTGTATGTGTAATGCTTTTGATGAAGTATCTTTTGAAGGTTTCTATTGCCTACCTATTTCGCTGCCCTTCTCTTTCCTTTTCTAATCACGCTCACTTTAGATTATTGCGGGTTTGTTTGAGTTCATAATAAGATCGATTCTTCCTTTGGTTATTATATTTGGATAATTTTGTATCATGTGTAAATGCTCGGATCTGTTTAAATACTTAGGCATTTCCGACAAATTTTTAATCCGTTTATTGATTTTGAATAATGATTAACATTATGTTGTTCGTGTAAGTAGATTGGTATGAGGGAGGATTTTGAAACTGGTTTCAAGGAATTGTTGGAGCAGGAGATATGCGTTGGCAATTCTTTACTTTATATTTGGTTGGCAGTGTTCCTCGAATCCAAGGGGAGGTTAAACGACGCTAACATAGTTTATGAACTTGGGTTGATGAGGTATCCCTATCTCTCTCTCATTGACAATTGACGTGCTGTTAAATACATGTAGATTATGCCTGTGTAAAATTATTTGTTGATTAGGATTTTTTCTTTGGTAGTGTTCTGTGTTGTTTTGTTCCTTACATCTTGGAGTGATAAACTGATAATAGGAACGCTGAGCCCCTTGAGTGGCTGAAAAAGGCAAAAACATTGTTTATTGATAGGATATCTGAATTGGTCAATGCTAATTCAATGCAAGTGGTACGTGATCTTTAAGCATAAAACCACTGAAGTTCATGTTCCTTCAATTGTATAATTTAAGAATTTTGACACGGTTGGTTTTCATTTTTGTAGAATGATGGCAGTGAATCCACTAAATTGGCAGAAAGTTACATCAACCCCTGGTCTTCCTCCATTATGAACGATTTATTAAGCAAGATTAATCCAAAGATTATGAAGTATGATGTAAGAAACTGCAGTATTCTTTTGCCAAATGATGAAAGCAAGCTGGTTATATTGTTCAAATAAATTACTCGCAAACTAACTTTACTATGAATTTAATGTGTGCTTCAGGGTTACCATTCAAGTACCAAAGTTTACTCTAAAAAGATGGCTCTAAAGTCATTGCGCAATTCATCAAGAAATAAGATTATTGAGATTGGTAATTTTATGTTATTCATCATAGTTAGAATTGTCACCTAAATGTATAAAACTACCTTCAGGTCCATCCTTCAAAAGACCGAGCTTTCTTGAAATATTGTGTAAGAAATGGAGGAAAAGATTTCTTATATCCCAGATTTGTTTTTCATTATAATAATTCACTTGCCAACATTTACAGGTGGAAAGAAGTATGAGATCAAAGGCTGTGCAGGTACAGGCGGTTTTGCTCAAGTATATAAATCATATCTTGACAGCAACCCAGACGATGTAGTAGCACTGAAGGTAAAAACTCTTATTGATTGACATTCAATCTGCATATGTCCTTTACTTCCAGCATTCCTTATAGGTTATACGTCATACCTGAATATGAACTAGTTGGATATTGTGATTCCTTTCAAATGCTTGTCAGATACAAACACCTCCTTTTCCTTGGGAGTTTTACATGTATCGTCAACTCGATCTACGTGTCACCGGGAAGCAAGTATGTAGTAGCCCTTATTTTCATTAAGCATTTATTACCTTTGGATTTAGCAACTCGATGTATACTCATCCTTGATCTCTCATAATGTCAGAGGTCAAGTTTTGGTTGTGCTCATGACATGTATCTCTATTCTGACTACAGCATACTTATATGTGATTACTTGGCTCATGGAACATTACAGGTGAGTTTTCAGATTCTGAACCCTTTTTATACTGTATGGTCCTTTAAAATATTCATTGTAAGTAACTGTCTAGTTAAAACCTTGTGCAGGATGCCATAAATTTGTATGTAGTCCTTGGAAAGACAATGGAAGAAGTTTTATGTATTTATTACACCATAGAGATGCTCTACATGCTAGAAACTTTGCACAGCATTGGCATTATCCATGGGGATTTTAAACCTGACAATCTGCTTATTCGTTATGCGGGGTAAAAATATTTTATTGTTCTAAATCTTGATTGATGACATCCATCTTTCTGAGAGTTGATTTCATTATATAGTTCCCAATTACAATACTTGTGAAAAAAAAAAGAACCGTAACAAATGAGAAGATGAGACCTCTAGCTGTTATTTACAACTTCAGTCTACCAATAATGATTAGCACCCGACTGGGGAGTGTACTCCAATTCAATTGTTTTGTACCAATTGATTTTATTTAACCGTTATGCAGAGACAGCCTAACAGAAGATGAATTCCAGGAACGTAGTGGTCCTTGGCAAGATCAGGTATTTAGTATTATCCCCGTTTTTGTTCCCAACTCATTATATGTCTAATTGGAATGACCTTGTAACTGCTGAAAAAAGTGTTTTTGAGTGGAAAAATGTGATTTTAGGCACTTTAAAGCCATTCCAAACCGACCCTACATTAACTGTGAGCAAACAACATTATTTGTAGCTTTTATATTGTAGGGGCTTTGCCTTGTTGATTGGGGAAGAGGTATAGATTTGCACCTTTTTCCTGAAAATGTAGAATTTAAAGGAGATTGCCGAACTTCTGGCTTTCGTTGTCCAGAAATGCTAGAGAATAAACCGTGGAAATATCAGGTCAGATTCTTCTGCAATGTGGTTATTTATTACAACTCCAAAGAAACCCCTCTGTAAAGAAGAAAACAGAAGCTCTACCAATAGCATTCTGTCTTTTGTACAGGTAGACATATATGGCTTTTGTGTAGTCGTCCACACAATGCTTCACGGTTCCTACATGGAAGTTGAGAAAAAAGCATCATCTGATGGCGACTTTGTTTATTTACCCAAATCATCTTTTAAGCGGTATTAGTTAACTCTGTCACCTTTAATTGTCTGTTTTCTGGGTTTTTCTCTTCCAATAAATATGTTCTTGAAATGCCTTGAATCGGTGTTCTGAATGACCAAGCATAGAGATCTTGCTGTCCAGTTAGTGAGTCAGGGTACACAACCTAAGTTGTATTTATATTTATGTTATTTTAGTTATTTATAAATTTGATCTTAAAGTTTAGAAGAATGCACTATATAAACTCTCTAACTCTACTAGCGCTTATGAATTTGTAATATGATATTTTCTCTCTAATAATAAATTGCTCTCTTCCTCTATAACGTAGCTTACACTGCTAGTGAACTACGTAAATCTGTGTGTGTTGATTCTCTATTGTTTATGTTGTATGTTTTCTTTATTTGTCGATTCCATAGCATAACAATTGTAACTCTGGTAGATTCTTTGCAGAGTTTCAGATCAGCAATTTTGCTTTTATAGAGAGCATCCATCCTCTTCACCTCCTCCCTCTGATTTGATCTCTCTGCCTCCATATAGGTATTGGAACATTGAACTATGGAAGAATCTCTTCACAAAGTTGCTTAATATCACACCAGGTGATTACAACAAAGAGTTGTTGGAGAGTATAAGGAAATCTTTCCAGGAATACATGTGCTCCAACCCTCAGCTTATAAGGAAGTTAAAGGAATTACTTGTAAAACAAAGAGCTTCCTTGTGTAATGCTTAA

mRNA sequence

ATGGCGATTTTCTTCGCCGATTCTCAAATCTCCACCACCACAGCATCCGCTCAGGACCCCTTGTTGCCTTGGCTCTGGTCCATCAAAGATGCTCTTGAAAATTCAACTTCAGGCAATGTTTCTGGGCCAGAACTCGCCAACCTTCTCTCTGATTGCATTGAAAATTTCAGCGGCAATGTCAAGTACAAAAACGATGTCCGATTTCTCAAGATTTGGTTTCTCTCTATTGGTATGAGGGAGGATTTTGAAACTGGTTTCAAGGAATTGTTGGAGCAGGAGATATGCGTTGGCAATTCTTTACTTTATATTTGGTTGGCAGTGTTCCTCGAATCCAAGGGGAGGTTAAACGACGCTAACATAGTTTATGAACTTGGGTTGATGAGGAACGCTGAGCCCCTTGAGTGGCTGAAAAAGGCAAAAACATTGTTTATTGATAGGATATCTGAATTGGTCAATGCTAATTCAATGCAAGTGAATGATGGCAGTGAATCCACTAAATTGGCAGAAAGTTACATCAACCCCTGGTCTTCCTCCATTATGAACGATTTATTAAGCAAGATTAATCCAAAGATTATGAAGTATGATGGTTACCATTCAAGTACCAAAGTTTACTCTAAAAAGATGGCTCTAAAGTCATTGCGCAATTCATCAAGAAATAAGATTATTGAGATTGGTGGAAAGAAGTATGAGATCAAAGGCTGTGCAGGTACAGGCGGTTTTGCTCAAGTATATAAATCATATCTTGACAGCAACCCAGACGATGTAGTAGCACTGAAGATACAAACACCTCCTTTTCCTTGGGAGTTTTACATGTATCGTCAACTCGATCTACGTGTCACCGGGAAGCAAAGGTCAAGTTTTGGTTGTGCTCATGACATGTATCTCTATTCTGACTACAGCATACTTATATGTGATTACTTGGCTCATGGAACATTACAGGATGCCATAAATTTGTATGTAGTCCTTGGAAAGACAATGGAAGAAGTTTTATGTATTTATTACACCATAGAGATGCTCTACATGCTAGAAACTTTGCACAGCATTGGCATTATCCATGGGGATTTTAAACCTGACAATCTGCTTATTCGTTATGCGGGAGACAGCCTAACAGAAGATGAATTCCAGGAACGTAGTGGTCCTTGGCAAGATCAGGGGCTTTGCCTTGTTGATTGGGGAAGAGGTATAGATTTGCACCTTTTTCCTGAAAATGTAGAATTTAAAGGAGATTGCCGAACTTCTGGCTTTCGTTGTCCAGAAATGCTAGAGAATAAACCGTGGAAATATCAGGTAGACATATATGGCTTTTGTGTAGTCGTCCACACAATGCTTCACGGTTCCTACATGGAAGTTGAGAAAAAAGCATCATCTGATGGCGACTTTGTTTATTTACCCAAATCATCTTTTAAGCGGTATTGGAACATTGAACTATGGAAGAATCTCTTCACAAAGTTGCTTAATATCACACCAGGTGATTACAACAAAGAGTTGTTGGAGAGTATAAGGAAATCTTTCCAGGAATACATGTGCTCCAACCCTCAGCTTATAAGGAAGTTAAAGGAATTACTTGTAAAACAAAGAGCTTCCTTGTGTAATGCTTAA

Coding sequence (CDS)

ATGGCGATTTTCTTCGCCGATTCTCAAATCTCCACCACCACAGCATCCGCTCAGGACCCCTTGTTGCCTTGGCTCTGGTCCATCAAAGATGCTCTTGAAAATTCAACTTCAGGCAATGTTTCTGGGCCAGAACTCGCCAACCTTCTCTCTGATTGCATTGAAAATTTCAGCGGCAATGTCAAGTACAAAAACGATGTCCGATTTCTCAAGATTTGGTTTCTCTCTATTGGTATGAGGGAGGATTTTGAAACTGGTTTCAAGGAATTGTTGGAGCAGGAGATATGCGTTGGCAATTCTTTACTTTATATTTGGTTGGCAGTGTTCCTCGAATCCAAGGGGAGGTTAAACGACGCTAACATAGTTTATGAACTTGGGTTGATGAGGAACGCTGAGCCCCTTGAGTGGCTGAAAAAGGCAAAAACATTGTTTATTGATAGGATATCTGAATTGGTCAATGCTAATTCAATGCAAGTGAATGATGGCAGTGAATCCACTAAATTGGCAGAAAGTTACATCAACCCCTGGTCTTCCTCCATTATGAACGATTTATTAAGCAAGATTAATCCAAAGATTATGAAGTATGATGGTTACCATTCAAGTACCAAAGTTTACTCTAAAAAGATGGCTCTAAAGTCATTGCGCAATTCATCAAGAAATAAGATTATTGAGATTGGTGGAAAGAAGTATGAGATCAAAGGCTGTGCAGGTACAGGCGGTTTTGCTCAAGTATATAAATCATATCTTGACAGCAACCCAGACGATGTAGTAGCACTGAAGATACAAACACCTCCTTTTCCTTGGGAGTTTTACATGTATCGTCAACTCGATCTACGTGTCACCGGGAAGCAAAGGTCAAGTTTTGGTTGTGCTCATGACATGTATCTCTATTCTGACTACAGCATACTTATATGTGATTACTTGGCTCATGGAACATTACAGGATGCCATAAATTTGTATGTAGTCCTTGGAAAGACAATGGAAGAAGTTTTATGTATTTATTACACCATAGAGATGCTCTACATGCTAGAAACTTTGCACAGCATTGGCATTATCCATGGGGATTTTAAACCTGACAATCTGCTTATTCGTTATGCGGGAGACAGCCTAACAGAAGATGAATTCCAGGAACGTAGTGGTCCTTGGCAAGATCAGGGGCTTTGCCTTGTTGATTGGGGAAGAGGTATAGATTTGCACCTTTTTCCTGAAAATGTAGAATTTAAAGGAGATTGCCGAACTTCTGGCTTTCGTTGTCCAGAAATGCTAGAGAATAAACCGTGGAAATATCAGGTAGACATATATGGCTTTTGTGTAGTCGTCCACACAATGCTTCACGGTTCCTACATGGAAGTTGAGAAAAAAGCATCATCTGATGGCGACTTTGTTTATTTACCCAAATCATCTTTTAAGCGGTATTGGAACATTGAACTATGGAAGAATCTCTTCACAAAGTTGCTTAATATCACACCAGGTGATTACAACAAAGAGTTGTTGGAGAGTATAAGGAAATCTTTCCAGGAATACATGTGCTCCAACCCTCAGCTTATAAGGAAGTTAAAGGAATTACTTGTAAAACAAAGAGCTTCCTTGTGTAATGCTTAA

Protein sequence

MAIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNVKYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSIMNDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEMLENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPGDYNKELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA
BLAST of Cla97C07G130810 vs. NCBI nr
Match: XP_008455277.1 (PREDICTED: mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucumis melo])

HSP 1 Score: 1025.8 bits (2651), Expect = 5.3e-296
Identity = 494/532 (92.86%), Postives = 509/532 (95.68%), Query Frame = 0

Query: 1   MAIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNV 60
           M I FADSQIS+TTASAQDPLLPWLWSIK ALENSTSGNVSG ELA LLSDCI NFSGNV
Sbjct: 1   MTISFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEIC+GNSLLYIWLA FLESKGRLNDANI
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120

Query: 121 VYELGLMRNAEPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSIM 180
           VYELGLMRNAEPLEWLKKAK LFIDRISELVN +SMQVND SESTK AESYINPWSSSIM
Sbjct: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180

Query: 181 NDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
           NDLL+KINPKIMKYDGYHSSTK YSKKM LKSLRNSSRNK IEIGGKKYEIKGCAGTGGF
Sbjct: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRV  K+RSSFGCAH MYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           ILICDYLA+GTLQDAIN YVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL
Sbjct: 301 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360

Query: 361 LIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEM 420
           L+RYAG++LTED+FQER GPWQDQGLCLVDWGRGIDL LFPENVEFKGDCRTSGFRCPEM
Sbjct: 361 LVRYAGENLTEDDFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LEN+PWKYQVDIYG CVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480

Query: 481 FTKLLNITPGDYNKELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           FTKLLNITPG YNKELL+S+RKSFQ+YMCSNPQLIRKLKELLVKQRASLC +
Sbjct: 481 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 532

BLAST of Cla97C07G130810 vs. NCBI nr
Match: XP_004136844.1 (PREDICTED: mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucumis sativus] >KGN43647.1 hypothetical protein Csa_7G050780 [Cucumis sativus])

HSP 1 Score: 1020.0 bits (2636), Expect = 2.9e-294
Identity = 493/532 (92.67%), Postives = 506/532 (95.11%), Query Frame = 0

Query: 1   MAIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNV 60
           MAIFFADSQISTTTAS QDPLLPWLWSIK ALENSTSGNVSG ELA LLSDCI NF GNV
Sbjct: 1   MAIFFADSQISTTTASPQDPLLPWLWSIKAALENSTSGNVSGSELAKLLSDCIGNFRGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEIC+ NSLLYIWLA FLESKGRLNDANI
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICISNSLLYIWLAAFLESKGRLNDANI 120

Query: 121 VYELGLMRNAEPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSIM 180
           VYELGLMRNAEPLEWLKKAK LFIDRISELVN +SMQVND SESTK AESYINPWSSSIM
Sbjct: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180

Query: 181 NDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
           +DLL+KI+PKIMKYDGYHSSTK YSKKM LKSLRNSSRNK IEIGGKKYEIKGCAGTGGF
Sbjct: 181 SDLLNKISPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRV  K+RSSFGCAH MYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAIN YVVLGKTMEEVLCIYYTIEMLYMLETLHSI IIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIDIIHGDFKPDNL 360

Query: 361 LIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEM 420
           LIRYAG+S+TED FQER GPWQDQGLCLVDWGRGIDL LFPENVEFKGDCRTSGFRCPEM
Sbjct: 361 LIRYAGESVTEDGFQERHGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LEN+PWKYQVDIYG CVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480

Query: 481 FTKLLNITPGDYNKELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           FTKLLNITPG YNKELL+S+RKSFQ+YMCSNPQLIRKLKELLVKQRASLC +
Sbjct: 481 FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 532

BLAST of Cla97C07G130810 vs. NCBI nr
Match: XP_023531077.1 (mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 985.3 bits (2546), Expect = 7.9e-284
Identity = 468/532 (87.97%), Postives = 498/532 (93.61%), Query Frame = 0

Query: 1   MAIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNV 60
           MAI F DSQI+TTT SAQDPLLPWLWSIKDALENSTSGNVSGP LA+L+SDCI+ F GNV
Sbjct: 1   MAIIFPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEIC+GNSLLYIW AVFLESKGRLNDAN+
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120

Query: 121 VYELGLMRNAEPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSIM 180
           +YELGL+RNAEPLEWLKKAK LFIDR+SELVN  SM++NDG ESTK+ E+Y+NPWSSS+M
Sbjct: 121 IYELGLLRNAEPLEWLKKAKMLFIDRMSELVNTRSMKLNDGGESTKIVETYVNPWSSSVM 180

Query: 181 NDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
            DLLSKIN KI+KY+GYHSS K Y KKMAL SLRNSSRNK+IEIGGKKYEIKGCAGTGGF
Sbjct: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFY+YRQLD RVT K+RSSFGCAH+MYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDHRVTVKERSSFGCAHEMYLYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLH+I IIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNISIIHGDFKPDNL 360

Query: 361 LIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEM 420
           LIRYA   LTED F+ERSGPW DQGLCLVDWGRGIDL LFPENVEF+GDCRTSGFRC EM
Sbjct: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LENKPWKYQVDIYGFCVVVHTMLHGSYM+VEKK SSDG FVYLPK+SFKRYWN+ELWKNL
Sbjct: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480

Query: 481 FTKLLNITPGDYNKELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           FTKLLNI  GDYNKELL+SIR+SFQ+YMCSNPQLIRKLKELL KQRASLC A
Sbjct: 481 FTKLLNIKLGDYNKELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 532

BLAST of Cla97C07G130810 vs. NCBI nr
Match: XP_022930700.1 (mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita moschata])

HSP 1 Score: 984.6 bits (2544), Expect = 1.4e-283
Identity = 468/532 (87.97%), Postives = 497/532 (93.42%), Query Frame = 0

Query: 1   MAIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNV 60
           MAI   DSQI+TTT SAQDPLLPWLWSIKDALENSTSGNVSGP LA+L+SDCI+ F G V
Sbjct: 58  MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 117

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEIC+GNSLLYIW AVFLESKGRLNDAN+
Sbjct: 118 KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 177

Query: 121 VYELGLMRNAEPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSIM 180
           +YELGL+RNAEPLEWLKKAK LFIDR+SELVN +SM++NDG ESTK  ESY+NPWSSS+M
Sbjct: 178 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 237

Query: 181 NDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
            DLLSKIN KI+KY+GYHSS K Y KKMAL SLRNSSRNK+IEIGGKKYEIKGCAGTGGF
Sbjct: 238 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 297

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFY+YRQLD RVT K+RSSFGCAH+MYLYSDYS
Sbjct: 298 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 357

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLH+IGIIHGDFKPDNL
Sbjct: 358 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 417

Query: 361 LIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEM 420
           LIRYA   LTED F+ERSGPW DQGLCLVDWGRGIDL LFPENVEF+GDCRTSGFRC EM
Sbjct: 418 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 477

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LENKPWKYQVDIYGFCVVVHTMLHGSYM+VEKK SSDG FVYLPK+SFKRYWN+ELWKNL
Sbjct: 478 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 537

Query: 481 FTKLLNITPGDYNKELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           FTKLLNI  GDYNKELL+SIR+SFQ+YMCSNPQLIRKLKELL KQRASLC A
Sbjct: 538 FTKLLNIKLGDYNKELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 589

BLAST of Cla97C07G130810 vs. NCBI nr
Match: XP_022989217.1 (mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita maxima])

HSP 1 Score: 978.0 bits (2527), Expect = 1.3e-281
Identity = 464/532 (87.22%), Postives = 495/532 (93.05%), Query Frame = 0

Query: 1   MAIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNV 60
           MAI F DSQI++TT S QDPLLPWLWSIKDALENSTSGNVSGP LA+L+SDCI+ F   V
Sbjct: 1   MAIIFPDSQITSTTTSVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEIC+GNSLLYIW AVFLESKGRLNDAN+
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120

Query: 121 VYELGLMRNAEPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSIM 180
           +YE+GL+RNAEPLEWLKKAK LFIDR+SELVN +SM++NDG ESTK  E+Y+NPWSSS+M
Sbjct: 121 IYEIGLLRNAEPLEWLKKAKMLFIDRMSELVNTHSMKLNDGGESTKFVETYVNPWSSSVM 180

Query: 181 NDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
            DLLSKIN KI+KY+GYHSS K Y KKMAL SLRNSSRNK+IEIGGKKYEIKGCAGTGGF
Sbjct: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFY+YRQLD RVT K+RSSFGCAH+MYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLH+IGIIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360

Query: 361 LIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEM 420
           LIRYA   LTED F+ERSGPW DQGLCLVDWGRGIDL LFPENVEF+GDCRTSGFRC EM
Sbjct: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LENKPWKYQVDIYGFCVVVHTMLHGSYM+VEKK SSDG FVYLPK+SFKRYWN+ELWKNL
Sbjct: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480

Query: 481 FTKLLNITPGDYNKELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           FTKLLNI  GDYNKELL+SIR SFQ+YMCSNPQLIRKLKELL KQRASLC A
Sbjct: 481 FTKLLNIKLGDYNKELLQSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA 532

BLAST of Cla97C07G130810 vs. TrEMBL
Match: tr|A0A1S3C1A0|A0A1S3C1A0_CUCME (mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucumis melo OX=3656 GN=LOC103495479 PE=4 SV=1)

HSP 1 Score: 1025.8 bits (2651), Expect = 3.5e-296
Identity = 494/532 (92.86%), Postives = 509/532 (95.68%), Query Frame = 0

Query: 1   MAIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNV 60
           M I FADSQIS+TTASAQDPLLPWLWSIK ALENSTSGNVSG ELA LLSDCI NFSGNV
Sbjct: 1   MTISFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEIC+GNSLLYIWLA FLESKGRLNDANI
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120

Query: 121 VYELGLMRNAEPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSIM 180
           VYELGLMRNAEPLEWLKKAK LFIDRISELVN +SMQVND SESTK AESYINPWSSSIM
Sbjct: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180

Query: 181 NDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
           NDLL+KINPKIMKYDGYHSSTK YSKKM LKSLRNSSRNK IEIGGKKYEIKGCAGTGGF
Sbjct: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRV  K+RSSFGCAH MYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           ILICDYLA+GTLQDAIN YVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL
Sbjct: 301 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360

Query: 361 LIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEM 420
           L+RYAG++LTED+FQER GPWQDQGLCLVDWGRGIDL LFPENVEFKGDCRTSGFRCPEM
Sbjct: 361 LVRYAGENLTEDDFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LEN+PWKYQVDIYG CVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480

Query: 481 FTKLLNITPGDYNKELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           FTKLLNITPG YNKELL+S+RKSFQ+YMCSNPQLIRKLKELLVKQRASLC +
Sbjct: 481 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 532

BLAST of Cla97C07G130810 vs. TrEMBL
Match: tr|A0A0A0K5T0|A0A0A0K5T0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G050780 PE=4 SV=1)

HSP 1 Score: 1020.0 bits (2636), Expect = 1.9e-294
Identity = 493/532 (92.67%), Postives = 506/532 (95.11%), Query Frame = 0

Query: 1   MAIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNV 60
           MAIFFADSQISTTTAS QDPLLPWLWSIK ALENSTSGNVSG ELA LLSDCI NF GNV
Sbjct: 1   MAIFFADSQISTTTASPQDPLLPWLWSIKAALENSTSGNVSGSELAKLLSDCIGNFRGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEIC+ NSLLYIWLA FLESKGRLNDANI
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICISNSLLYIWLAAFLESKGRLNDANI 120

Query: 121 VYELGLMRNAEPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSIM 180
           VYELGLMRNAEPLEWLKKAK LFIDRISELVN +SMQVND SESTK AESYINPWSSSIM
Sbjct: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180

Query: 181 NDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
           +DLL+KI+PKIMKYDGYHSSTK YSKKM LKSLRNSSRNK IEIGGKKYEIKGCAGTGGF
Sbjct: 181 SDLLNKISPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRV  K+RSSFGCAH MYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAIN YVVLGKTMEEVLCIYYTIEMLYMLETLHSI IIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIDIIHGDFKPDNL 360

Query: 361 LIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEM 420
           LIRYAG+S+TED FQER GPWQDQGLCLVDWGRGIDL LFPENVEFKGDCRTSGFRCPEM
Sbjct: 361 LIRYAGESVTEDGFQERHGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LEN+PWKYQVDIYG CVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480

Query: 481 FTKLLNITPGDYNKELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           FTKLLNITPG YNKELL+S+RKSFQ+YMCSNPQLIRKLKELLVKQRASLC +
Sbjct: 481 FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 532

BLAST of Cla97C07G130810 vs. TrEMBL
Match: tr|A0A2P5DNI9|A0A2P5DNI9_9ROSA (Mitotic spindle checkpoint protein Bub1/Mad OS=Trema orientalis OX=63057 GN=TorRG33x02_246260 PE=4 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 2.1e-216
Identity = 362/532 (68.05%), Postives = 439/532 (82.52%), Query Frame = 0

Query: 1   MAIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNV 60
           MAI + +SQ  T   S  DPLLPWLWSI+ ALE+  SGN SG +L+ L+SDCI+ F  + 
Sbjct: 1   MAI-YPESQAPT---SLHDPLLPWLWSIRKALEDLKSGNDSGLDLSKLVSDCIKMFKDSA 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANI 120
           +Y++D+RFLKIWFL IG  +DF + F E+LE++ICVG SLLY+W A ++ESKG+L DA +
Sbjct: 61  QYRDDIRFLKIWFLHIGFCKDFGSIFTEMLEKKICVGQSLLYVWYASYIESKGKLYDAQM 120

Query: 121 VYELGLMRNAEPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSIM 180
           VY++G++RNAEP+EWLKKA+ LF+DR+SELVN++S++  D  ES +  ES+INPWSSS M
Sbjct: 121 VYQMGILRNAEPVEWLKKAQALFLDRVSELVNSSSLEKVDDGESIQSEESHINPWSSSTM 180

Query: 181 NDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
            +LL + N +I KYDG  S  K YS K+AL S +N SRNKIIEIGG+KY+IKG AG GGF
Sbjct: 181 KNLLRRTNTQITKYDGCRSINKPYSGKVALSS-QNLSRNKIIEIGGRKYQIKGRAGQGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYS 300
           AQVYK+ L SNPD++VALKIQ PPFPWEFYMYRQLD R+  + RSSFG A  M+LYSD S
Sbjct: 241 AQVYKACLISNPDEIVALKIQKPPFPWEFYMYRQLDQRILAEDRSSFGFAERMHLYSDCS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           IL+CDYLA+GTLQDAIN YVV GK+MEEVLCIYYTIEMLYMLETLH  GIIHGDFKPDNL
Sbjct: 301 ILVCDYLANGTLQDAINSYVVTGKSMEEVLCIYYTIEMLYMLETLHGGGIIHGDFKPDNL 360

Query: 361 LIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEM 420
           LIRYA D L ED F++RSG W+DQGLCLVDWGRGIDL LFP+++EFKGDCRTSGFRC EM
Sbjct: 361 LIRYARDDLVEDGFRDRSGSWRDQGLCLVDWGRGIDLRLFPDDMEFKGDCRTSGFRCIEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
            +NKPWK+QVD YG CV+VH MLH SYME+++K S DG ++YLPKS FKRYWN++LWKN 
Sbjct: 421 QQNKPWKFQVDTYGLCVIVHMMLHNSYMEIQEKPSPDGGYIYLPKSPFKRYWNVDLWKNF 480

Query: 481 FTKLLNITPGDYNKELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           FTKLLN  PG+  + LL+ +R+SFQ+YMCSNPQLIRKLKELL+KQRASLC+A
Sbjct: 481 FTKLLNNCPGNNERNLLQDLRESFQDYMCSNPQLIRKLKELLLKQRASLCSA 527

BLAST of Cla97C07G130810 vs. TrEMBL
Match: tr|A0A2I4E644|A0A2I4E644_9ROSI (mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1 OS=Juglans regia OX=51240 GN=LOC108986629 PE=4 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 2.1e-216
Identity = 370/534 (69.29%), Postives = 430/534 (80.52%), Query Frame = 0

Query: 1   MAIFFADSQISTTTASAQDPLLPWLWSIKDALENST-SGNVSGPELANLLSDCIENFSGN 60
           M + F D      T SA DPLLPWLWSIK ALE  T SGN SG +L+ LL DCI  F  +
Sbjct: 1   MTVVFED------TNSAPDPLLPWLWSIKKALEAETSSGNGSGLDLSKLLYDCIVTFRDD 60

Query: 61  VKYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDAN 120
            +Y+NDVRFL+IWFL + + EDFE+ F+E+ E EIC+G+SLLY+W A FLESKG+L+DA+
Sbjct: 61  DQYQNDVRFLRIWFLYMDVSEDFESIFREMTESEICLGHSLLYVWYASFLESKGKLHDAH 120

Query: 121 IVYELGLMRNAEPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSI 180
           +VY+ G +RNA+P+E LKKA  LF+DR+S LVNA S+Q  D   S +   S+INPWS+S 
Sbjct: 121 MVYQSGFLRNAKPVELLKKAHALFLDRMSGLVNACSLQKIDDCGSIEFETSHINPWSAST 180

Query: 181 MNDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGG 240
           M DLL K+ P+IMKYDGYHSS K YS K+AL SL+NSSRNKIIEIGGKKY+I GCAG GG
Sbjct: 181 MKDLLKKLGPQIMKYDGYHSSQKAYSGKVALSSLQNSSRNKIIEIGGKKYQITGCAGQGG 240

Query: 241 FAQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDY 300
           FAQVYK+Y+ SNPD+VVALKIQ P FPWEFYMYRQLD R+   +RS+FG A  M+LYSD 
Sbjct: 241 FAQVYKAYVHSNPDEVVALKIQKPAFPWEFYMYRQLDERILDNERSNFGFALRMHLYSDC 300

Query: 301 SILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDN 360
           SIL+CDYLAHGTLQDAIN YVV GK+MEE LCIYYT+EMLYMLETLH  GIIHGDFKPDN
Sbjct: 301 SILVCDYLAHGTLQDAINSYVVTGKSMEEELCIYYTVEMLYMLETLHGAGIIHGDFKPDN 360

Query: 361 LLIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPE 420
           LLIRYA   LTED  +E SGPW DQGLCLVDWGRGID  LFPEN EFKGDCRTSGFRC E
Sbjct: 361 LLIRYARGDLTEDGLREHSGPWHDQGLCLVDWGRGIDKRLFPENAEFKGDCRTSGFRCIE 420

Query: 421 MLENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKN 480
           M ENKPW +QVD YG CV+VH MLH SYME+EKK SS G ++YLPKSS KRYW +ELWK+
Sbjct: 421 MQENKPWTFQVDTYGLCVIVHMMLHNSYMEIEKKKSSGGGYIYLPKSSLKRYWKVELWKD 480

Query: 481 LFTKLLNITPG-DYNKELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           LFT+LLN++PG D NK  L  +R+SFQ+YMCS PQ I+KLKELLVKQRASLC+A
Sbjct: 481 LFTQLLNMSPGSDDNK--LRKLRESFQDYMCSKPQFIKKLKELLVKQRASLCSA 526

BLAST of Cla97C07G130810 vs. TrEMBL
Match: tr|A0A061E527|A0A061E527_THECC (ATP binding,protein kinases,protein serine/threonine kinases isoform 1 OS=Theobroma cacao OX=3641 GN=TCM_008143 PE=4 SV=1)

HSP 1 Score: 757.7 bits (1955), Expect = 1.8e-215
Identity = 353/522 (67.62%), Postives = 428/522 (81.99%), Query Frame = 0

Query: 11  STTTASAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNVKYKNDVRFLK 70
           S  ++SA DPL PWL SIK AL+   SGN +G +L NLLSDCI  F  N KY+ND+RFLK
Sbjct: 8   SNDSSSAHDPLFPWLTSIKKALDEWYSGNRTGADLDNLLSDCISTFKHNAKYRNDLRFLK 67

Query: 71  IWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANIVYELGLMRNA 130
           IWF+ +   +DFE+ F+E+ E EIC+G+SLLY W A FLE+KG+  +A+ VY++G+ R A
Sbjct: 68  IWFIYLEGSKDFESVFREIEENEICIGHSLLYEWYAYFLEAKGKWKEAHTVYQIGISRKA 127

Query: 131 EPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSIMNDLLSKINPK 190
           +PLE LK A++LF++R+S+ +N +S    DGSE  +  + +INPWS+  + +L  KI+P+
Sbjct: 128 DPLEKLKGAQSLFVERMSDRLNGSSFGKIDGSEPAEFGKKFINPWSTFTIEELSKKIHPQ 187

Query: 191 IMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGFAQVYKSYLDS 250
           I KYDGYH S KVYS K+AL SL+ SSRNKIIEIGGKKY+IKGCAG GGFAQV+K+Y+DS
Sbjct: 188 ITKYDGYHLSKKVYSGKVALSSLKKSSRNKIIEIGGKKYQIKGCAGQGGFAQVFKAYIDS 247

Query: 251 NPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYSILICDYLAHG 310
           NPDDVVALKIQ P FPWEFYMYRQLD R+  KQRS+FG A  +++YSD SIL+CDYL+HG
Sbjct: 248 NPDDVVALKIQKPAFPWEFYMYRQLDERILDKQRSNFGSAQKIHIYSDCSILVCDYLSHG 307

Query: 311 TLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDSLT 370
           TLQDAIN YVV GK+MEEVLCIYYT EMLYMLETLH +GIIHGDFKPDNLLIRY+ D L+
Sbjct: 308 TLQDAINSYVVTGKSMEEVLCIYYTTEMLYMLETLHRVGIIHGDFKPDNLLIRYSKDDLS 367

Query: 371 EDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEMLENKPWKYQV 430
           ED F++R+GPW DQGLCLVDWGRGIDLHLFP+N EF GDCRTSGFRC +M E KPW +QV
Sbjct: 368 EDGFKDRTGPWHDQGLCLVDWGRGIDLHLFPDNTEFTGDCRTSGFRCIQMQEKKPWTFQV 427

Query: 431 DIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPG 490
           D YG CVVVH MLH +YME+EKKAS D D++YLPKSSFKRYWN+ LWK+LFTKLLN++PG
Sbjct: 428 DTYGLCVVVHMMLHNTYMEIEKKASDD-DYIYLPKSSFKRYWNVNLWKDLFTKLLNVSPG 487

Query: 491 DYNKELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           + + ELL+S+RKSF EYMC NP L+ KLKELLVKQRASLC A
Sbjct: 488 NNDAELLQSLRKSFLEYMCDNPPLVNKLKELLVKQRASLCCA 528

BLAST of Cla97C07G130810 vs. Swiss-Prot
Match: sp|F4IVI0|BUB1_ARATH (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Arabidopsis thaliana OX=3702 GN=BUB1 PE=1 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 2.5e-196
Identity = 317/518 (61.20%), Postives = 405/518 (78.19%), Query Frame = 0

Query: 16  SAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNVKYKNDVRFLKIWFLS 75
           +A DPL PWL  IK+++E+  +G  SG +L  LL DCI  +  + +Y+ND+RFLKIWFL 
Sbjct: 8   AAGDPLFPWLMEIKNSMEDLYAGKNSGYDLDKLLFDCISTYKKDSRYRNDLRFLKIWFLY 67

Query: 76  IGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANIVYELGLMRNAEPLEW 135
           +  REDFE  ++E+ E EIC G+SLLY W A+FLE KG    AN VY+ GL R AEP + 
Sbjct: 68  LEGREDFERVYREIEETEICKGHSLLYEWYAIFLEVKGLWRRANSVYQTGLSRKAEPFDR 127

Query: 136 LKKAKTLFIDRISELVNANSMQ-VNDGSESTKLAESYINPWSSSIMNDLLSKINPKIMKY 195
           LK+A +LF+ RIS+   A+S++ V D +++T L   ++NPW +S +N L+ KI P+++KY
Sbjct: 128 LKEAHSLFLQRISKRTKASSLEKVGDDAQATDLETGFVNPWETSTVNGLIHKIKPQLVKY 187

Query: 196 DGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDD 255
           DGYH S KV+  K  L SL+N SRNKIIEIGG+KY++KGCAG GGFAQV+K+++DSNPD+
Sbjct: 188 DGYHVSNKVFPGKANLSSLQNYSRNKIIEIGGRKYQMKGCAGQGGFAQVFKAFIDSNPDE 247

Query: 256 VVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYSILICDYLAHGTLQD 315
           VVALK+Q PPFPWEF+MYRQLD R+   QRSSFG A  +++YSDYSIL+CDYL+HGTLQD
Sbjct: 248 VVALKVQKPPFPWEFHMYRQLDCRIPDSQRSSFGLAQRVHVYSDYSILVCDYLSHGTLQD 307

Query: 316 AINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDSLTEDEF 375
            IN YVV+GK+MEEVLC+YYTIEML MLETLHS+GIIHGDFKPDNLLIRY  ++LTE  F
Sbjct: 308 VINSYVVVGKSMEEVLCMYYTIEMLNMLETLHSVGIIHGDFKPDNLLIRYPPENLTETGF 367

Query: 376 QERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEMLENKPWKYQVDIYG 435
            E++G W  +GLCLVDWGRGIDL LFP   EF GDCRTSGFRC EM E+KPWK+QVD YG
Sbjct: 368 HEKTGSWSKKGLCLVDWGRGIDLSLFPRTTEFTGDCRTSGFRCVEMKEDKPWKFQVDTYG 427

Query: 436 FCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPGDYNK 495
            CV+VH MLH  YME+EKK S DG ++ +P++SFKRYW ++LWK LFTKLLN    + + 
Sbjct: 428 LCVIVHMMLHNVYMEIEKKQSLDGGYINMPRTSFKRYWKVDLWKELFTKLLNRETCEDDT 487

Query: 496 ELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           E L ++RKS +EY+CS+P+L++KL ELL KQR SLC++
Sbjct: 488 ETLRNLRKSMEEYICSDPKLMKKLNELLAKQRISLCSS 525

BLAST of Cla97C07G130810 vs. Swiss-Prot
Match: sp|O08901|BUB1_MOUSE (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Mus musculus OX=10090 GN=Bub1 PE=1 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 2.7e-49
Identity = 117/354 (33.05%), Postives = 189/354 (53.39%), Query Frame = 0

Query: 173  NPWSSSIMNDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIK 232
            NPW   ++  LLS ++  +  Y    ++ +  SK  A+K+          ++G     + 
Sbjct: 714  NPWDDELILKLLSGLSKPVTSYS---NTFEWQSKLPAIKTKTE------YQLGSLLVYVN 773

Query: 233  GCAGTGGFAQVYKS----YLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFG 292
               G G FAQV+++      ++  +    LK+Q P   WEFY+  QL  R+  +    F 
Sbjct: 774  HLLGEGAFAQVFEAIHGDVRNAKSEQKCILKVQRPANSWEFYIGMQLMERLKPEVHHMFI 833

Query: 293  CAHDMYLYSDYSILICDYLAHGTLQDAINLYV-VLGKTMEEVLCIYYTIEMLYMLETLHS 352
              +  +L+ + SIL+ +  ++GTL + INLY     K M + L + + I MLYM+E +HS
Sbjct: 834  KFYSAHLFKNGSILVGELYSYGTLLNVINLYKNTSEKVMPQALVLTFAIRMLYMVEQVHS 893

Query: 353  IGIIHGDFKPDNLLIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFK 412
              IIHGD KPDN ++ +         F E++      GL L+D G+ ID+ LFP+   F 
Sbjct: 894  CEIIHGDIKPDNFILGH--------RFLEQADEDLATGLALIDLGQSIDMKLFPKGTVFT 953

Query: 413  GDCRTSGFRCPEMLENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSS 472
            G C TSGF+CPEML NKPW YQ+D +G    ++ ML GSYM+V+ +       V+ P+  
Sbjct: 954  GKCETSGFQCPEMLSNKPWNYQIDYFGVAATIYCMLFGSYMKVKNEGG-----VWKPEGL 1013

Query: 473  FKRYWNIELWKNLFTKLLNITPGDYNKELLESIRKSFQEYMCSNPQLIRKLKEL 522
            F+R  ++++W+  F  +LNI P  +N   L+ +R++ ++ +    Q   K+K L
Sbjct: 1014 FRRLPHLDMWEEFFHIMLNI-PDCHNLPSLDFLRQNMKKLL--EQQYSNKIKTL 1042


HSP 2 Score: 55.1 bits (131), Expect = 2.8e-06
Identity = 39/133 (29.32%), Postives = 58/133 (43.61%), Query Frame = 0

Query: 16  SAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNVKYKNDVRFLKIWFLS 75
           +  DPL  W   IK   EN          L  LL   ++ F     Y ND RF+      
Sbjct: 19  TGNDPLGEWESFIKWVEENFPDNK---EYLMTLLEHLMKEFLHKKNYHNDSRFINYCLKF 78

Query: 76  IGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANIVYELGLMRNAEPLEW 135
                D    F+ L  Q I   +S +Y+  A  LE++G L  A+ +++ G+   AEP E 
Sbjct: 79  AEYNSDRHQFFEFLYNQGIGTKSSYIYMSWAGHLEAQGELQHASAIFQTGIHNEAEPKEL 138

Query: 136 LKKAKTLFIDRIS 149
           L++   LF  R++
Sbjct: 139 LQQQYRLFQARLT 148

BLAST of Cla97C07G130810 vs. Swiss-Prot
Match: sp|P41695|BUB1_YEAST (Checkpoint serine/threonine-protein kinase BUB1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=BUB1 PE=1 SV=2)

HSP 1 Score: 196.4 bits (498), Expect = 7.8e-49
Identity = 109/327 (33.33%), Postives = 179/327 (54.74%), Query Frame = 0

Query: 173 NPWSSSIMNDLLSKINPKIMKYDGYHSSTKVYSKKMALKSL----RNSSRNKIIEI--GG 232
           NP S+++    LS+I+P + +Y+ +++  +       LK +    RN ++N I++    G
Sbjct: 643 NPLSNNLRAKFLSEISPPLFQYNTFYNYNQELKMSSLLKKIHRVSRNENKNPIVDFKKTG 702

Query: 233 KKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQ-RS 292
             Y I+G  G GG+A VY +  +S+   + ALK++ P   WE+Y+  Q++ R+       
Sbjct: 703 DLYCIRGELGEGGYATVYLA--ESSQGHLRALKVEKPASVWEYYIMSQVEFRLRKSTILK 762

Query: 293 SFGCAHDMYLYSDYSILICDYLAHGTLQDAINLY----VVLGKTMEEVLCIYYTIEMLYM 352
           S   A  ++L+ D S L+ +Y + GT+ D INL     +     M+E LC++ T+E++ +
Sbjct: 763 SIINASALHLFLDESYLVLNYASQGTVLDLINLQREKAIDGNGIMDEYLCMFITVELMKV 822

Query: 353 LETLHSIGIIHGDFKPDNLLIRY--AGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHL 412
           LE +H +GIIHGD KPDN +IR    G+ L     +     W+++G+ L+D+GR  D+ L
Sbjct: 823 LEKIHEVGIIHGDLKPDNCMIRLEKPGEPLGAHYMRNGEDGWENKGIYLIDFGRSFDMTL 882

Query: 413 FPENVEFKGDCRTSGFRCPEMLENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGD 472
            P   +FK + +     C EM   KPW Y+ D YG   V+H+ML G ++E  +  +    
Sbjct: 883 LPPGTKFKSNWKADQQDCWEMRAGKPWSYEADYYGLAGVIHSMLFGKFIETIQLQNGRCK 942

Query: 473 FVYLPKSSFKRYWNIELWKNLFTKLLN 487
                K+ FKRYW  E+W  +F  LLN
Sbjct: 943 L----KNPFKRYWKKEIWGVIFDLLLN 963

BLAST of Cla97C07G130810 vs. Swiss-Prot
Match: sp|O43683|BUB1_HUMAN (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Homo sapiens OX=9606 GN=BUB1 PE=1 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 2.5e-47
Identity = 115/359 (32.03%), Postives = 184/359 (51.25%), Query Frame = 0

Query: 173  NPWSSSIMNDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIK 232
            NPW   ++  LLS ++  +  Y         +  +  L +++  +     ++G K   + 
Sbjct: 740  NPWDDKLIFKLLSGLSKPVSSY------PNTFEWQCKLPAIKPKTE---FQLGSKLVYVH 799

Query: 233  GCAGTGGFAQVYKS----YLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFG 292
               G G FAQVY++      D+       LK+Q P  PWEFY+  QL  R+    +  F 
Sbjct: 800  HLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMERLKPSMQHMFM 859

Query: 293  CAHDMYLYSDYSILICDYLAHGTLQDAINLYV-VLGKTMEEVLCIYYTIEMLYMLETLHS 352
              +  +L+ + S+L+ +  ++GTL +AINLY     K M + L I + + MLYM+E +H 
Sbjct: 860  KFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVHD 919

Query: 353  IGIIHGDFKPDNLLIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFK 412
              IIHGD KPDN ++      L +D+  + S      GL L+D G+ ID+ LFP+   F 
Sbjct: 920  CEIIHGDIKPDNFIL--GNGFLEQDDEDDLSA-----GLALIDLGQSIDMKLFPKGTIFT 979

Query: 413  GDCRTSGFRCPEMLENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSS 472
              C TSGF+C EML NKPW YQ+D +G    V+ ML G+YM+V+ +          P+  
Sbjct: 980  AKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYMKVKNEGGE-----CKPEGL 1039

Query: 473  FKRYWNIELWKNLFTKLLNITPGDYNKE---LLESIRKSFQEYMCSNPQLIRKLKELLV 524
            F+R  ++++W   F  +LNI    +      L + ++K FQ++  +    IR L+  L+
Sbjct: 1040 FRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKVFQQHYTNK---IRALRNRLI 1074

BLAST of Cla97C07G130810 vs. Swiss-Prot
Match: sp|O94751|BUB1_SCHPO (Checkpoint serine/threonine-protein kinase bub1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bub1 PE=1 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 2.2e-43
Identity = 126/417 (30.22%), Postives = 197/417 (47.24%), Query Frame = 0

Query: 155  SMQVNDGSESTKLAESYINPWSSSIMNDLLSKINPKIMKYDGYHSS-------------- 214
            +M   +   S     + INP   S+ + L   + P +++   YH                
Sbjct: 623  TMPYEEAMASLSNLPTLINPLDQSLRDLLFQVLRPSLLRDKDYHEHETSFALVEHIESFV 682

Query: 215  TKVYSKKMALKSLRNSSRNK-------IIEIGGKKYEIKGCAGTGGFAQVY--KSYLDSN 274
            +K+  K       R+S+R+        +         +    G G FA VY  KS +++ 
Sbjct: 683  SKIKPKAGGPGRRRSSNRHSLDGPEFHLFYPPNTNLSVISKLGQGAFAPVYLVKSKIETE 742

Query: 275  PDDV------------VALKIQTPPFPWEFYMYRQLDLRVTG-KQRSSFGCAHDMYLYSD 334
              DV             ALKI+TPP  +EFY+ RQ   R+ G ++ +S    H ++++ D
Sbjct: 743  NGDVSQGGAENNESKLFALKIETPPSCFEFYLTRQAMTRLKGLRETNSILPVHQLHMFHD 802

Query: 335  YSILICDYLAHGTLQDAINLY---VVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDF 394
             S L+ DY   G++ D +N           M+E+L ++++IE L ++E LH+  IIHGD 
Sbjct: 803  TSHLLMDYRPQGSILDLVNSMHNSTFSSSGMDEILVVFFSIEFLRIIEALHTHKIIHGDL 862

Query: 395  KPDNLLIRYAGDSLTEDEFQERSGP-----WQDQGLCLVDWGRGIDLHLFPENVEFKGDC 454
            K DN L+R   +++ + E+     P     W  +G+ L+D+GRGIDL LF E V+F  D 
Sbjct: 863  KADNALLRL--ETVADSEWSPIYSPEGLYGWSFKGIYLIDFGRGIDLSLFEEKVKFIADW 922

Query: 455  RTSGFRCPEMLENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKR 514
             T    C EM E +PW YQ+D +G   +++TML G Y+E   +   +G    +     KR
Sbjct: 923  DTDLQDCIEMREGRPWTYQIDYHGLAAIIYTMLFGQYIETRIEV-INGQRRQVLTQRMKR 982

Query: 515  YWNIELWKNLFTKLLNITPGDYNKEL-----LESIRKSFQEYMCSNPQLIRKLKELL 523
            YWN +LW  LF  LLN T     + L     L  IR   +E++ ++      LK LL
Sbjct: 983  YWNQDLWHRLFDLLLNPTLHVSEENLPMTEELSKIRIEMEEWLVNHSTGGSGLKGLL 1036

BLAST of Cla97C07G130810 vs. TAIR10
Match: AT2G20635.1 (ATP binding;protein kinases;protein serine/threonine kinases)

HSP 1 Score: 686.4 bits (1770), Expect = 1.4e-197
Identity = 317/518 (61.20%), Postives = 405/518 (78.19%), Query Frame = 0

Query: 16  SAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNVKYKNDVRFLKIWFLS 75
           +A DPL PWL  IK+++E+  +G  SG +L  LL DCI  +  + +Y+ND+RFLKIWFL 
Sbjct: 8   AAGDPLFPWLMEIKNSMEDLYAGKNSGYDLDKLLFDCISTYKKDSRYRNDLRFLKIWFLY 67

Query: 76  IGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANIVYELGLMRNAEPLEW 135
           +  REDFE  ++E+ E EIC G+SLLY W A+FLE KG    AN VY+ GL R AEP + 
Sbjct: 68  LEGREDFERVYREIEETEICKGHSLLYEWYAIFLEVKGLWRRANSVYQTGLSRKAEPFDR 127

Query: 136 LKKAKTLFIDRISELVNANSMQ-VNDGSESTKLAESYINPWSSSIMNDLLSKINPKIMKY 195
           LK+A +LF+ RIS+   A+S++ V D +++T L   ++NPW +S +N L+ KI P+++KY
Sbjct: 128 LKEAHSLFLQRISKRTKASSLEKVGDDAQATDLETGFVNPWETSTVNGLIHKIKPQLVKY 187

Query: 196 DGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDD 255
           DGYH S KV+  K  L SL+N SRNKIIEIGG+KY++KGCAG GGFAQV+K+++DSNPD+
Sbjct: 188 DGYHVSNKVFPGKANLSSLQNYSRNKIIEIGGRKYQMKGCAGQGGFAQVFKAFIDSNPDE 247

Query: 256 VVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYSILICDYLAHGTLQD 315
           VVALK+Q PPFPWEF+MYRQLD R+   QRSSFG A  +++YSDYSIL+CDYL+HGTLQD
Sbjct: 248 VVALKVQKPPFPWEFHMYRQLDCRIPDSQRSSFGLAQRVHVYSDYSILVCDYLSHGTLQD 307

Query: 316 AINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDSLTEDEF 375
            IN YVV+GK+MEEVLC+YYTIEML MLETLHS+GIIHGDFKPDNLLIRY  ++LTE  F
Sbjct: 308 VINSYVVVGKSMEEVLCMYYTIEMLNMLETLHSVGIIHGDFKPDNLLIRYPPENLTETGF 367

Query: 376 QERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEMLENKPWKYQVDIYG 435
            E++G W  +GLCLVDWGRGIDL LFP   EF GDCRTSGFRC EM E+KPWK+QVD YG
Sbjct: 368 HEKTGSWSKKGLCLVDWGRGIDLSLFPRTTEFTGDCRTSGFRCVEMKEDKPWKFQVDTYG 427

Query: 436 FCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPGDYNK 495
            CV+VH MLH  YME+EKK S DG ++ +P++SFKRYW ++LWK LFTKLLN    + + 
Sbjct: 428 LCVIVHMMLHNVYMEIEKKQSLDGGYINMPRTSFKRYWKVDLWKELFTKLLNRETCEDDT 487

Query: 496 ELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           E L ++RKS +EY+CS+P+L++KL ELL KQR SLC++
Sbjct: 488 ETLRNLRKSMEEYICSDPKLMKKLNELLAKQRISLCSS 525

BLAST of Cla97C07G130810 vs. TAIR10
Match: AT5G05510.1 (Mad3/BUB1 homology region 1)

HSP 1 Score: 76.6 bits (187), Expect = 5.0e-14
Identity = 39/132 (29.55%), Postives = 67/132 (50.76%), Query Frame = 0

Query: 16  SAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNVKYKNDVRFLKIWFLS 75
           S +D LLPW+  +K  ++ S    +   +L   L  C E+F  + +YKND R++++W   
Sbjct: 23  SGKDHLLPWIRGVK-KMKESLPSQILNEKLPRFLQKCAESFESDKRYKNDSRYIRVWLQL 82

Query: 76  IGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANIVYELGLMRNAEPLEW 135
           +   +D     + +  + I    SL Y   A+  E   R  DA  +Y LG+   AEP++ 
Sbjct: 83  MDFVDDPRALLRTMEAKSIGTKRSLFYQAYALHYEKMKRFEDAEKMYRLGVQNLAEPMDE 142

Query: 136 LKKAKTLFIDRI 148
           L+K+   F+ R+
Sbjct: 143 LQKSYLQFVTRM 153

BLAST of Cla97C07G130810 vs. TAIR10
Match: AT2G33560.2 (BUB1-related (BUB1: budding uninhibited by benzymidazol 1))

HSP 1 Score: 71.6 bits (174), Expect = 1.6e-12
Identity = 52/187 (27.81%), Postives = 88/187 (47.06%), Query Frame = 0

Query: 19  DPLLPWLWSIKDALEN-STSGNVSGPELANLLSDCIENFSGNVKYKNDVRFLKIWFLSIG 78
           DPL PW+  IK   E     G  SG  L  +   C+  F  + +YK+D+R+LK+W     
Sbjct: 80  DPLSPWIECIKWVQEAFPPGGECSG--LLVIYEQCVRKFWHSERYKDDLRYLKVWLEYAE 139

Query: 79  MREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANIVYELGLMRNAEPLEWLK 138
              D E  +K L   EI   +++ YI  A+ +E K ++  AN ++ LG+ R+A+P+E L 
Sbjct: 140 HCADAEVIYKFLEVNEIGKTHAVYYIAYALHIEFKNKVKTANEIFNLGISRDAKPVEKLN 199

Query: 139 KAKTLFIDRISELVNANSMQVNDGSESTKLAE----SYINPWSSSIMNDLLSKINPKIMK 198
            A   F+  +  +  +N+    +  E+  L      + ++   ++     L   NP+  K
Sbjct: 200 DAYKKFM--VRTMRRSNTADEQEPKENNDLPSRSFGTLLSRGDNNARRQALGSSNPQAKK 259

Query: 199 YDGYHSS 201
                SS
Sbjct: 260 LKPNQSS 262

BLAST of Cla97C07G130810 vs. TAIR10
Match: AT2G25090.1 (CBL-interacting protein kinase 16)

HSP 1 Score: 60.8 bits (146), Expect = 2.8e-09
Identity = 59/262 (22.52%), Postives = 108/262 (41.22%), Query Frame = 0

Query: 228 KYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGK---QR 287
           KY I    GTG FA+VY     S  DDV    I+         M  Q++  +      + 
Sbjct: 14  KYNIGRLLGTGNFAKVYHGTEISTGDDVAIKVIKKDHVFKRRGMMEQIEREIAVMRLLRH 73

Query: 288 SSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLET 347
            +     ++         + +Y+  G L + I+     GK  E++   Y+  +++  ++ 
Sbjct: 74  PNVVELREVMATKKKIFFVMEYVNGGELFEMIDRD---GKLPEDLARKYFQ-QLISAVDF 133

Query: 348 LHSIGIIHGDFKPDNLLIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENV 407
            HS G+ H D KP+NLL+   GD               D GL  +    G+       + 
Sbjct: 134 CHSRGVFHRDIKPENLLLDGEGDLKV-----------TDFGLSALMMPEGLGGRRGSSDD 193

Query: 408 EFKGDCRTSGFRCPEMLENKPWK-YQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYL 467
                C T  +  PE+L NK +     DI+   +V++ +L G    +++   +    ++ 
Sbjct: 194 LLHTRCGTPAYVAPEVLRNKGYDGAMADIWSCGIVLYALLAGFLPFIDENVMTLYTKIFK 253

Query: 468 PKSSFKRYWNIELWKNLFTKLL 486
            +  F  ++++E  K L ++LL
Sbjct: 254 AECEFPPWFSLES-KELLSRLL 259

BLAST of Cla97C07G130810 vs. TAIR10
Match: AT2G26980.4 (CBL-interacting protein kinase 3)

HSP 1 Score: 57.0 bits (136), Expect = 4.1e-08
Identity = 68/262 (25.95%), Postives = 112/262 (42.75%), Query Frame = 0

Query: 211 KSLRNSSRNKIIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQTPPFPWEFY 270
           K LR  +R + ++    KYE+    G G FA+V K   +S   + VALKI       +  
Sbjct: 6   KKLREMNRRQQVKRRVGKYEVGRTIGEGTFAKV-KFARNSETGEPVALKILDKEKVLKHK 65

Query: 271 MYRQLDLRVTGKQRSSFGCAHDMY--LYSDYSI-LICDYLAHGTLQDAINLYVVLGKTME 330
           M  Q+   +   +         +Y  + S   I +I +Y+  G L D I    V    M+
Sbjct: 66  MAEQIRREIATMKLIKHPNVVQLYEVMASKTKIFIILEYVTGGELFDKI----VNDGRMK 125

Query: 331 EVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLI-RYAGDSLTEDEFQERSGPWQDQGL 390
           E     Y  ++++ ++  HS G+ H D KP+NLL+  Y    +++      S   +D GL
Sbjct: 126 EDEARRYFQQLIHAVDYCHSRGVYHRDLKPENLLLDSYGNLKISDFGLSALSQQVRDDGL 185

Query: 391 CLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEMLENKPWK-YQVDIYGFCVVVHTMLHG 450
                     LH           C T  +  PE+L ++ +     D++   VV++ +L G
Sbjct: 186 ----------LHT---------SCGTPNYVAPEVLNDRGYDGATADMWSCGVVLYVLLAG 242

Query: 451 -------SYMEVEKKASSDGDF 461
                  + M + KK SS G+F
Sbjct: 246 YLPFDDSNLMNLYKKISS-GEF 242

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008455277.15.3e-29692.86PREDICTED: mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucumis melo... [more]
XP_004136844.12.9e-29492.67PREDICTED: mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucumis sati... [more]
XP_023531077.17.9e-28487.97mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita pepo subsp. p... [more]
XP_022930700.11.4e-28387.97mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita moschata][more]
XP_022989217.11.3e-28187.22mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
tr|A0A1S3C1A0|A0A1S3C1A0_CUCME3.5e-29692.86mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucumis melo OX=3656 ... [more]
tr|A0A0A0K5T0|A0A0A0K5T0_CUCSA1.9e-29492.67Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G050780 PE=4 SV=1[more]
tr|A0A2P5DNI9|A0A2P5DNI9_9ROSA2.1e-21668.05Mitotic spindle checkpoint protein Bub1/Mad OS=Trema orientalis OX=63057 GN=TorR... [more]
tr|A0A2I4E644|A0A2I4E644_9ROSI2.1e-21669.29mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1 OS=Juglans re... [more]
tr|A0A061E527|A0A061E527_THECC1.8e-21567.62ATP binding,protein kinases,protein serine/threonine kinases isoform 1 OS=Theobr... [more]
Match NameE-valueIdentityDescription
sp|F4IVI0|BUB1_ARATH2.5e-19661.20Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Arabidopsis thaliana ... [more]
sp|O08901|BUB1_MOUSE2.7e-4933.05Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Mus musculus OX=10090... [more]
sp|P41695|BUB1_YEAST7.8e-4933.33Checkpoint serine/threonine-protein kinase BUB1 OS=Saccharomyces cerevisiae (str... [more]
sp|O43683|BUB1_HUMAN2.5e-4732.03Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Homo sapiens OX=9606 ... [more]
sp|O94751|BUB1_SCHPO2.2e-4330.22Checkpoint serine/threonine-protein kinase bub1 OS=Schizosaccharomyces pombe (st... [more]
Match NameE-valueIdentityDescription
AT2G20635.11.4e-19761.20ATP binding;protein kinases;protein serine/threonine kinases[more]
AT5G05510.15.0e-1429.55Mad3/BUB1 homology region 1[more]
AT2G33560.21.6e-1227.81BUB1-related (BUB1: budding uninhibited by benzymidazol 1)[more]
AT2G25090.12.8e-0922.52CBL-interacting protein kinase 16[more]
AT2G26980.44.1e-0825.95CBL-interacting protein kinase 3[more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0007094mitotic spindle assembly checkpoint
GO:0006468protein phosphorylation
Vocabulary: Molecular Function
TermDefinition
GO:0004672protein kinase activity
GO:0005524ATP binding
Vocabulary: INTERPRO
TermDefinition
IPR011009Kinase-like_dom_sf
IPR008271Ser/Thr_kinase_AS
IPR015661Bub1/Mad3
IPR013212Mad3/Bub1_I
IPR000719Prot_kinase_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0006468 protein phosphorylation
biological_process GO:0007094 mitotic spindle assembly checkpoint
biological_process GO:0051754 meiotic sister chromatid cohesion, centromeric
cellular_component GO:0005575 cellular_component
cellular_component GO:0000778 condensed nuclear chromosome kinetochore
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0003723 RNA binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C07G130810.1Cla97C07G130810.1mRNA


Analysis Name: InterPro Annotations of watermelon 97103 v2
Date Performed: 2019-05-12
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 229..522
e-value: 1.5E-9
score: 2.0
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 231..448
e-value: 3.4E-17
score: 62.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 229..532
score: 18.549
IPR013212Mad3/Bub1 homology region 1SMARTSM00777mad3_bub1_icoord: 8..126
e-value: 2.0E-10
score: 47.2
IPR013212Mad3/Bub1 homology region 1PFAMPF08311Mad3_BUB1_Icoord: 9..126
e-value: 4.2E-21
score: 75.1
IPR013212Mad3/Bub1 homology region 1PROSITEPS51489BUB1_Ncoord: 6..167
score: 33.354
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 226..529
e-value: 4.8E-98
score: 329.4
NoneNo IPR availableGENE3DG3DSA:1.25.40.430coord: 5..150
e-value: 3.4E-29
score: 103.5
NoneNo IPR availablePANTHERPTHR14030:SF6MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1coord: 19..532
IPR015661Mitotic spindle checkpoint protein Bub1/Mad3PANTHERPTHR14030MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1coord: 19..532
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 350..362
IPR011009Protein kinase-like domain superfamilySUPERFAMILYSSF56112Protein kinase-like (PK-like)coord: 226..489

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cla97C07G130810Cucurbita maxima (Rimu)cmawmbB541
Cla97C07G130810Cucurbita moschata (Rifu)cmowmbB127
Cla97C07G130810Cucurbita moschata (Rifu)cmowmbB522
Cla97C07G130810Cucurbita moschata (Rifu)cmowmbB525