Cla002231 (gene) Watermelon (97103) v1

BLAST of Cla002231 vs. Swiss-Prot
Match: BUB1_ARATH (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Arabidopsis thaliana GN=BUB1 PE=1 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 2.3e-194
Identity = 317/518 (61.20%), Postives = 403/518 (77.80%), Query Frame = 1

Query: 16  SAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNVKYKNDVRFLKIWFLS 75
           +A DPL PWL  IK+++E+  +G  SG +L  LL DCI  +  + +Y+ND+RFLKIWFL 
Sbjct: 8   AAGDPLFPWLMEIKNSMEDLYAGKNSGYDLDKLLFDCISTYKKDSRYRNDLRFLKIWFLY 67

Query: 76  IGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANIVYELGLMRNAEPLEW 135
           +  REDFE  ++E+ E EIC G+SLLY W A+FLE KG    AN VY+ GL R AEP + 
Sbjct: 68  LEGREDFERVYREIEETEICKGHSLLYEWYAIFLEVKGLWRRANSVYQTGLSRKAEPFDR 127

Query: 136 LKKAKTLFIDRISELVNANSMQ-VNDGSESTKLAESYINPWSSSIMNDLLSKINPKIMKY 195
           LK+A +LF+ RIS+   A+S++ V D +++T L   ++NPW +S +N L+ KI P+++KY
Sbjct: 128 LKEAHSLFLQRISKRTKASSLEKVGDDAQATDLETGFVNPWETSTVNGLIHKIKPQLVKY 187

Query: 196 DGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDD 255
           DGYH S KV+  K  L SL+N SRNKIIEIGG+KY++KGCAG GGFAQV+K+++DSNPD+
Sbjct: 188 DGYHVSNKVFPGKANLSSLQNYSRNKIIEIGGRKYQMKGCAGQGGFAQVFKAFIDSNPDE 247

Query: 256 VVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYSILICDYLAHGTLQD 315
           VVALK+Q PPFPWEF+MYRQLD R+   QRSSFG A  +++YSDYSIL+CDYL+HGTLQD
Sbjct: 248 VVALKVQKPPFPWEFHMYRQLDCRIPDSQRSSFGLAQRVHVYSDYSILVCDYLSHGTLQD 307

Query: 316 AINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDSLTEDEF 375
            IN YVV+GK+MEEVLC+YYTIEML MLETLHS+GIIHGDFKPDNLLIRY  ++LTE  F
Sbjct: 308 VINSYVVVGKSMEEVLCMYYTIEMLNMLETLHSVGIIHGDFKPDNLLIRYPPENLTETGF 367

Query: 376 QERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEMLENKPWKYQVDIYG 435
            E++G W  +GLCLVDWGRGIDL LFP   EF GDCRTSGFRC EM E+KPWK+QVD YG
Sbjct: 368 HEKTGSWSKKGLCLVDWGRGIDLSLFPRTTEFTGDCRTSGFRCVEMKEDKPWKFQVDTYG 427

Query: 436 FCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPGDYNK 495
            CV+VH MLH  YME+EKK S DG ++ +P++SFKRYW ++LWK LFTKLLN    + + 
Sbjct: 428 LCVIVHMMLHNVYMEIEKKQSLDGGYINMPRTSFKRYWKVDLWKELFTKLLNRETCEDDT 487

Query: 496 ELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           E L ++RKS +EY+CS+P+L++KL ELL KQR SLC++
Sbjct: 488 ETLRNLRKSMEEYICSDPKLMKKLNELLAKQRISLCSS 525

BLAST of Cla002231 vs. Swiss-Prot
Match: BUB1_MOUSE (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Mus musculus GN=Bub1 PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.9e-48
Identity = 117/354 (33.05%), Postives = 188/354 (53.11%), Query Frame = 1

Query: 173  NPWSSSIMNDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIK 232
            NPW   ++  LLS ++  +  Y    ++ +  SK  A+K+          ++G     + 
Sbjct: 714  NPWDDELILKLLSGLSKPVTSYS---NTFEWQSKLPAIKTKTE------YQLGSLLVYVN 773

Query: 233  GCAGTGGFAQVYKSY----LDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFG 292
               G G FAQV+++      ++  +    LK+Q P   WEFY+  QL  R+  +    F 
Sbjct: 774  HLLGEGAFAQVFEAIHGDVRNAKSEQKCILKVQRPANSWEFYIGMQLMERLKPEVHHMFI 833

Query: 293  CAHDMYLYSDYSILICDYLAHGTLQDAINLYVVLG-KTMEEVLCIYYTIEMLYMLETLHS 352
              +  +L+ + SIL+ +  ++GTL + INLY     K M + L + + I MLYM+E +HS
Sbjct: 834  KFYSAHLFKNGSILVGELYSYGTLLNVINLYKNTSEKVMPQALVLTFAIRMLYMVEQVHS 893

Query: 353  IGIIHGDFKPDNLLIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFK 412
              IIHGD KPDN ++ +         F E++      GL L+D G+ ID+ LFP+   F 
Sbjct: 894  CEIIHGDIKPDNFILGH--------RFLEQADEDLATGLALIDLGQSIDMKLFPKGTVFT 953

Query: 413  GDCRTSGFRCPEMLENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSS 472
            G C TSGF+CPEML NKPW YQ+D +G    ++ ML GSYM+V+ +       V+ P+  
Sbjct: 954  GKCETSGFQCPEMLSNKPWNYQIDYFGVAATIYCMLFGSYMKVKNEGG-----VWKPEGL 1013

Query: 473  FKRYWNIELWKNLFTKLLNITPGDYNKELLESIRKSFQEYMCSNPQLIRKLKEL 522
            F+R  ++++W+  F  +LNI P  +N   L+ +R++ ++ +    Q   K+K L
Sbjct: 1014 FRRLPHLDMWEEFFHIMLNI-PDCHNLPSLDFLRQNMKKLL--EQQYSNKIKTL 1042

HSP 2 Score: 52.4 bits (124), Expect = 1.8e-05
Identity = 39/133 (29.32%), Postives = 56/133 (42.11%), Query Frame = 1

Query: 16  SAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNVKYKNDVRFLKIWFLS 75
           +  DPL  W   IK   EN          L  LL   ++ F     Y ND RF+      
Sbjct: 19  TGNDPLGEWESFIKWVEENFPDNK---EYLMTLLEHLMKEFLHKKNYHNDSRFINYCLKF 78

Query: 76  IGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANIVYELGLMRNAEPLEW 135
                D    F+ L  Q I   +S +Y+  A  LE++G L  A+ +++ G+   AEP E 
Sbjct: 79  AEYNSDRHQFFEFLYNQGIGTKSSYIYMSWAGHLEAQGELQHASAIFQTGIHNEAEPKEL 138

Query: 136 LKKAKTLFIDRIS 149
           L++   LF  R++
Sbjct: 139 LQQQYRLFQARLT 148

BLAST of Cla002231 vs. Swiss-Prot
Match: BUB1_YEAST (Checkpoint serine/threonine-protein kinase BUB1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BUB1 PE=1 SV=2)

HSP 1 Score: 192.6 bits (488), Expect = 1.1e-47
Identity = 110/327 (33.64%), Postives = 178/327 (54.43%), Query Frame = 1

Query: 173 NPWSSSIMNDLLSKINPKIMKYDGYHSSTKVYSKKMALKSL----RNSSRNKIIEIG--G 232
           NP S+++    LS+I+P + +Y+ +++  +       LK +    RN ++N I++    G
Sbjct: 643 NPLSNNLRAKFLSEISPPLFQYNTFYNYNQELKMSSLLKKIHRVSRNENKNPIVDFKKTG 702

Query: 233 KKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQ-RS 292
             Y I+G  G GG+A VY +  +S+   + ALK++ P   WE+Y+  Q++ R+       
Sbjct: 703 DLYCIRGELGEGGYATVYLA--ESSQGHLRALKVEKPASVWEYYIMSQVEFRLRKSTILK 762

Query: 293 SFGCAHDMYLYSDYSILICDYLAHGTLQDAINLY---VVLGK-TMEEVLCIYYTIEMLYM 352
           S   A  ++L+ D S L+ +Y + GT+ D INL     + G   M+E LC++ T+E++ +
Sbjct: 763 SIINASALHLFLDESYLVLNYASQGTVLDLINLQREKAIDGNGIMDEYLCMFITVELMKV 822

Query: 353 LETLHSIGIIHGDFKPDNLLIRYA--GDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHL 412
           LE +H +GIIHGD KPDN +IR    G+ L     +     W+++G+ L+D+GR  D+ L
Sbjct: 823 LEKIHEVGIIHGDLKPDNCMIRLEKPGEPLGAHYMRNGEDGWENKGIYLIDFGRSFDMTL 882

Query: 413 FPENVEFKGDCRTSGFRCPEMLENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGD 472
            P   +FK + +     C EM   KPW Y+ D YG   V+H+ML G ++E  +  +    
Sbjct: 883 LPPGTKFKSNWKADQQDCWEMRAGKPWSYEADYYGLAGVIHSMLFGKFIETIQLQNGRCK 942

Query: 473 FVYLPKSSFKRYWNIELWKNLFTKLLN 487
                K+ FKRYW  E+W  +F  LLN
Sbjct: 943 L----KNPFKRYWKKEIWGVIFDLLLN 963

HSP 2 Score: 51.6 bits (122), Expect = 3.1e-05
Identity = 74/262 (28.24%), Postives = 108/262 (41.22%), Query Frame = 1

Query: 8   SQISTTTASAQDPLLPWLWSIKDALEN--------STS----GNVSGPE-LANLLSDCIE 67
           SQ++ T  + +  LL  L  + D L+         STS     + SG E L + +  C+ 
Sbjct: 34  SQLNQTKIAYEQRLLNDLEDMDDPLDLFLDYMIWISTSYIEVDSESGQEVLRSTMERCLI 93

Query: 68  NFSGNVKYKNDVRFLKIW------FLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVF 127
                  Y+ND RFLKIW      FLS    E  E  FK +  + I    SL Y   +  
Sbjct: 94  YIQDMETYRNDPRFLKIWIWYINLFLSNNFHES-ENTFKYMFNKGIGTKLSLFYEEFSKL 153

Query: 128 LESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISEL-VNANSMQVNDGSESTK- 187
           LE+     +A ++ ELG   N  P   L ++ + + DR+ E+ +  N   V D  E  K 
Sbjct: 154 LENAQFFLEAKVLLELGAENNCRPYNRLLRSLSNYEDRLREMNIVENQNSVPDSRERLKG 213

Query: 188 -----LAESYINPW-SSSIMND-------LLSKINPKIMKYDGYHSSTKVYSKKMALKSL 232
                 A  +I  + +SS+M D       L S +       + Y  S  V   K   + L
Sbjct: 214 RLIYRTAPFFIRKFLTSSLMTDDKENRANLNSNVGVGKSAPNVYQDSIVVADFKSETERL 273

BLAST of Cla002231 vs. Swiss-Prot
Match: BUB1_HUMAN (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Homo sapiens GN=BUB1 PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 3.6e-46
Identity = 115/359 (32.03%), Postives = 182/359 (50.70%), Query Frame = 1

Query: 173  NPWSSSIMNDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIK 232
            NPW   ++  LLS ++  +  Y         +  +  L +++  +     ++G K   + 
Sbjct: 740  NPWDDKLIFKLLSGLSKPVSSYPN------TFEWQCKLPAIKPKTE---FQLGSKLVYVH 799

Query: 233  GCAGTGGFAQVYKSYL----DSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFG 292
               G G FAQVY++      D+       LK+Q P  PWEFY+  QL  R+    +  F 
Sbjct: 800  HLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMERLKPSMQHMFM 859

Query: 293  CAHDMYLYSDYSILICDYLAHGTLQDAINLYV-VLGKTMEEVLCIYYTIEMLYMLETLHS 352
              +  +L+ + S+L+ +  ++GTL +AINLY     K M + L I + + MLYM+E +H 
Sbjct: 860  KFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVHD 919

Query: 353  IGIIHGDFKPDNLLIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFK 412
              IIHGD KPDN ++      L +D+  + S      GL L+D G+ ID+ LFP+   F 
Sbjct: 920  CEIIHGDIKPDNFIL--GNGFLEQDDEDDLSA-----GLALIDLGQSIDMKLFPKGTIFT 979

Query: 413  GDCRTSGFRCPEMLENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSS 472
              C TSGF+C EML NKPW YQ+D +G    V+ ML G+YM+V+ +          P+  
Sbjct: 980  AKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYMKVKNEGGE-----CKPEGL 1039

Query: 473  FKRYWNIELWKNLFTKLLNITPGDYNKE---LLESIRKSFQEYMCSNPQLIRKLKELLV 524
            F+R  ++++W   F  +LNI    +      L + ++K FQ++  +    IR L+  L+
Sbjct: 1040 FRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKVFQQHYTNK---IRALRNRLI 1074

HSP 2 Score: 55.1 bits (131), Expect = 2.8e-06
Identity = 42/131 (32.06%), Postives = 56/131 (42.75%), Query Frame = 1

Query: 19  DPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNVKYKNDVRFLKIWFLSIGM 78
           DPL  W   I+   EN          L  LL   ++ F    KY ND RF+         
Sbjct: 22  DPLGEWERYIQWVEENFPENK---EYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEY 81

Query: 79  REDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANIVYELGLMRNAEPLEWLKK 138
             D    F+ L    I   +S LYI  A  LE++G L  A+ V + G+   AEP E+L++
Sbjct: 82  NSDLHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRGIQNQAEPREFLQQ 141

Query: 139 AKTLFIDRISE 150
              LF  R++E
Sbjct: 142 QYRLFQTRLTE 149

BLAST of Cla002231 vs. Swiss-Prot
Match: BUB1_SCHPO (Checkpoint serine/threonine-protein kinase bub1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=bub1 PE=1 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.6e-41
Identity = 111/315 (35.24%), Postives = 164/315 (52.06%), Query Frame = 1

Query: 236  GTGGFAQVY--KSYLDSNPDDV------------VALKIQTPPFPWEFYMYRQLDLRVTG 295
            G G FA VY  KS +++   DV             ALKI+TPP  +EFY+ RQ   R+ G
Sbjct: 725  GQGAFAPVYLVKSKIETENGDVSQGGAENNESKLFALKIETPPSCFEFYLTRQAMTRLKG 784

Query: 296  -KQRSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLY---VVLGKTMEEVLCIYYTIE 355
             ++ +S    H ++++ D S L+ DY   G++ D +N           M+E+L ++++IE
Sbjct: 785  LRETNSILPVHQLHMFHDTSHLLMDYRPQGSILDLVNSMHNSTFSSSGMDEILVVFFSIE 844

Query: 356  MLYMLETLHSIGIIHGDFKPDNLLIRYAGDSLTEDEFQERSGP-----WQDQGLCLVDWG 415
             L ++E LH+  IIHGD K DN L+R   +++ + E+     P     W  +G+ L+D+G
Sbjct: 845  FLRIIEALHTHKIIHGDLKADNALLRL--ETVADSEWSPIYSPEGLYGWSFKGIYLIDFG 904

Query: 416  RGIDLHLFPENVEFKGDCRTSGFRCPEMLENKPWKYQVDIYGFCVVVHTMLHGSYMEVEK 475
            RGIDL LF E V+F  D  T    C EM E +PW YQ+D +G   +++TML G Y+E   
Sbjct: 905  RGIDLSLFEEKVKFIADWDTDLQDCIEMREGRPWTYQIDYHGLAAIIYTMLFGQYIETRI 964

Query: 476  KASSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPGDYNKEL-----LESIRKSFQEY 523
            +   +G    +     KRYWN +LW  LF  LLN T     + L     L  IR   +E+
Sbjct: 965  EV-INGQRRQVLTQRMKRYWNQDLWHRLFDLLLNPTLHVSEENLPMTEELSKIRIEMEEW 1024

BLAST of Cla002231 vs. TrEMBL
Match: A0A0A0K5T0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G050780 PE=4 SV=1)

HSP 1 Score: 1013.8 bits (2620), Expect = 7.5e-293
Identity = 493/532 (92.67%), Postives = 506/532 (95.11%), Query Frame = 1

Query: 1   MAIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNV 60
           MAIFFADSQISTTTAS QDPLLPWLWSIK ALENSTSGNVSG ELA LLSDCI NF GNV
Sbjct: 1   MAIFFADSQISTTTASPQDPLLPWLWSIKAALENSTSGNVSGSELAKLLSDCIGNFRGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEIC+ NSLLYIWLA FLESKGRLNDANI
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICISNSLLYIWLAAFLESKGRLNDANI 120

Query: 121 VYELGLMRNAEPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSIM 180
           VYELGLMRNAEPLEWLKKAK LFIDRISELVN +SMQVND SESTK AESYINPWSSSIM
Sbjct: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180

Query: 181 NDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
           +DLL+KI+PKIMKYDGYHSSTK YSKKM LKSLRNSSRNK IEIGGKKYEIKGCAGTGGF
Sbjct: 181 SDLLNKISPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRV  K+RSSFGCAH MYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAIN YVVLGKTMEEVLCIYYTIEMLYMLETLHSI IIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIDIIHGDFKPDNL 360

Query: 361 LIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEM 420
           LIRYAG+S+TED FQER GPWQDQGLCLVDWGRGIDL LFPENVEFKGDCRTSGFRCPEM
Sbjct: 361 LIRYAGESVTEDGFQERHGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LEN+PWKYQVDIYG CVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480

Query: 481 FTKLLNITPGDYNKELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           FTKLLNITPG YNKELL+S+RKSFQ+YMCSNPQLIRKLKELLVKQRASLC +
Sbjct: 481 FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 532

BLAST of Cla002231 vs. TrEMBL
Match: A0A061E527_THECC (ATP binding,protein kinases,protein serine/threonine kinases isoform 1 OS=Theobroma cacao GN=TCM_008143 PE=4 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 5.3e-214
Identity = 353/522 (67.62%), Postives = 428/522 (81.99%), Query Frame = 1

Query: 11  STTTASAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNVKYKNDVRFLK 70
           S  ++SA DPL PWL SIK AL+   SGN +G +L NLLSDCI  F  N KY+ND+RFLK
Sbjct: 8   SNDSSSAHDPLFPWLTSIKKALDEWYSGNRTGADLDNLLSDCISTFKHNAKYRNDLRFLK 67

Query: 71  IWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANIVYELGLMRNA 130
           IWF+ +   +DFE+ F+E+ E EIC+G+SLLY W A FLE+KG+  +A+ VY++G+ R A
Sbjct: 68  IWFIYLEGSKDFESVFREIEENEICIGHSLLYEWYAYFLEAKGKWKEAHTVYQIGISRKA 127

Query: 131 EPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSIMNDLLSKINPK 190
           +PLE LK A++LF++R+S+ +N +S    DGSE  +  + +INPWS+  + +L  KI+P+
Sbjct: 128 DPLEKLKGAQSLFVERMSDRLNGSSFGKIDGSEPAEFGKKFINPWSTFTIEELSKKIHPQ 187

Query: 191 IMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGFAQVYKSYLDS 250
           I KYDGYH S KVYS K+AL SL+ SSRNKIIEIGGKKY+IKGCAG GGFAQV+K+Y+DS
Sbjct: 188 ITKYDGYHLSKKVYSGKVALSSLKKSSRNKIIEIGGKKYQIKGCAGQGGFAQVFKAYIDS 247

Query: 251 NPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYSILICDYLAHG 310
           NPDDVVALKIQ P FPWEFYMYRQLD R+  KQRS+FG A  +++YSD SIL+CDYL+HG
Sbjct: 248 NPDDVVALKIQKPAFPWEFYMYRQLDERILDKQRSNFGSAQKIHIYSDCSILVCDYLSHG 307

Query: 311 TLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDSLT 370
           TLQDAIN YVV GK+MEEVLCIYYT EMLYMLETLH +GIIHGDFKPDNLLIRY+ D L+
Sbjct: 308 TLQDAINSYVVTGKSMEEVLCIYYTTEMLYMLETLHRVGIIHGDFKPDNLLIRYSKDDLS 367

Query: 371 EDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEMLENKPWKYQV 430
           ED F++R+GPW DQGLCLVDWGRGIDLHLFP+N EF GDCRTSGFRC +M E KPW +QV
Sbjct: 368 EDGFKDRTGPWHDQGLCLVDWGRGIDLHLFPDNTEFTGDCRTSGFRCIQMQEKKPWTFQV 427

Query: 431 DIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPG 490
           D YG CVVVH MLH +YME+EKKAS D D++YLPKSSFKRYWN+ LWK+LFTKLLN++PG
Sbjct: 428 DTYGLCVVVHMMLHNTYMEIEKKASDD-DYIYLPKSSFKRYWNVNLWKDLFTKLLNVSPG 487

Query: 491 DYNKELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           + + ELL+S+RKSF EYMC NP L+ KLKELLVKQRASLC A
Sbjct: 488 NNDAELLQSLRKSFLEYMCDNPPLVNKLKELLVKQRASLCCA 528

BLAST of Cla002231 vs. TrEMBL
Match: E0CTG3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0028g03360 PE=4 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 1.4e-209
Identity = 356/545 (65.32%), Postives = 426/545 (78.17%), Query Frame = 1

Query: 11  STTTASAQDPLLPWLW-----------------------SIKDALENSTSGNVSGPELAN 70
           ST  AS  DPLLPWLW                       SIK AL+   S + +G +L N
Sbjct: 8   STNDASGNDPLLPWLWYSLSLSLCFLSHEFSHNVLIKSRSIKKALDGFNSSHKNGKDLEN 67

Query: 71  LLSDCIENFSGNVKYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAV 130
           LLS+CI  F GN +Y+ND RFLKIWFL +  REDF + F+E+ E +ICVG SLLY   A+
Sbjct: 68  LLSNCITTFKGNPQYRNDPRFLKIWFLYLDAREDFASIFREMEENKICVGQSLLYESYAL 127

Query: 131 FLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKL 190
           FLE+KG+L DA +VY++G+ R AEPL+ LKKA+ LF+DR+ E+VNA S++  D  ES +L
Sbjct: 128 FLEAKGKLLDAFMVYQIGVSRKAEPLDKLKKAQALFMDRMKEIVNACSLEPIDDGESAEL 187

Query: 191 AESYINPWSSSIMNDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGK 250
             S+ NPWS S + DLL K  P+I+KYDGYH STK YS+K+AL SL+NSSRNKIIEIGG 
Sbjct: 188 GTSFTNPWSISTIKDLLQKKKPQILKYDGYHPSTKAYSRKVALSSLQNSSRNKIIEIGGY 247

Query: 251 KYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSF 310
           KY+IKGCAG GGFAQV+K+Y++ NPD+VVALKIQ P FPWEFYMYRQLD R+  K+RSSF
Sbjct: 248 KYQIKGCAGKGGFAQVFKAYVNYNPDEVVALKIQKPAFPWEFYMYRQLDKRIPDKERSSF 307

Query: 311 GCAHDMYLYSDYSILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHS 370
           G AH ++LYSD SIL+ +YLAHGTLQDAIN YVV+G +MEEVLCIYYTIEMLYMLET+HS
Sbjct: 308 GFAHRLHLYSDCSILVSEYLAHGTLQDAINSYVVIGGSMEEVLCIYYTIEMLYMLETIHS 367

Query: 371 IGIIHGDFKPDNLLIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFK 430
           +GIIHGDFKPDNLLIRY+   LTE+EF ER GPW+DQGLCLVDWGRGIDL LFP N EFK
Sbjct: 368 VGIIHGDFKPDNLLIRYSRHDLTEEEFHERCGPWRDQGLCLVDWGRGIDLSLFPSNTEFK 427

Query: 431 GDCRTSGFRCPEMLENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSS 490
           GDCRTSGFRC EM E+KPWK+Q   YG CV+VH MLH SYM +EKK S DG ++Y PKS 
Sbjct: 428 GDCRTSGFRCVEMQEHKPWKFQAYTYGLCVIVHMMLHNSYMAIEKKPSFDGGYIYQPKSP 487

Query: 491 FKRYWNIELWKNLFTKLLNITPGDYNKELLESIRKSFQEYMCSNPQLIRKLKELLVKQRA 533
           FKRYWN++LWKNLFTKLLN +P + +  LL+++R+SFQ+YMCSNPQLI+KLK LLVKQRA
Sbjct: 488 FKRYWNVQLWKNLFTKLLNSSPHEDHSRLLQNLRESFQDYMCSNPQLIKKLKHLLVKQRA 547

BLAST of Cla002231 vs. TrEMBL
Match: M5WUD8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa018571mg PE=4 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 3.0e-209
Identity = 358/559 (64.04%), Postives = 432/559 (77.28%), Query Frame = 1

Query: 1   MAIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNV 60
           MA+ F DSQ ST     QDPLLPWLWS+K ALE+  SG  SG +L  LLSDCI  F  N 
Sbjct: 1   MAVIFPDSQPSTPL---QDPLLPWLWSVKQALEDWNSGKESGSDLTKLLSDCIAAFKDND 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANI 120
           +Y++D+RFLKIWF  +   EDF + F+++ E +ICVGN+LLYIW A FLESKG+L DA++
Sbjct: 61  QYRDDIRFLKIWFFYMDFSEDFGSVFQKMWESKICVGNALLYIWYASFLESKGKLYDASM 120

Query: 121 VYELGLMRNAEPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSIM 180
           VY++G+ RNA+P+EW+KKA  LF++R+SE+V++     ND S +     S  NPWSSS M
Sbjct: 121 VYQMGISRNAKPVEWVKKAHALFLERMSEIVSSAQTIGNDESITFG---SLTNPWSSSTM 180

Query: 181 NDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
             LL KIN ++MKYDGY  +TK YS K+AL SL+NSSRNK IEIG  KY+I GCAG GGF
Sbjct: 181 KVLLKKINHELMKYDGYQCTTKPYSGKVALSSLKNSSRNKTIEIGRTKYQIIGCAGQGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYS 300
           AQVYK+Y+  NPDDVVALKIQ P FPWEFYMYRQL+ R++ K+RSSFG AH M+LYSD S
Sbjct: 241 AQVYKAYVSCNPDDVVALKIQKPAFPWEFYMYRQLNQRISDKERSSFGVAHRMHLYSDCS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           IL+CDYLA+GTLQDAIN + V+GK+MEEVLCIYYTIEMLYMLETLH +GIIHGDFKPDNL
Sbjct: 301 ILVCDYLANGTLQDAINSFAVIGKSMEEVLCIYYTIEMLYMLETLHGVGIIHGDFKPDNL 360

Query: 361 LIRYA-----------------------------GDSLTEDEFQERSGPWQDQGLCLVDW 420
           LIRYA                             G+ LT+D F++RSGPW DQGLCLVDW
Sbjct: 361 LIRYARLTYEAYHLATSMTCPFGRDGITKAGSRYGNDLTKDGFRDRSGPWHDQGLCLVDW 420

Query: 421 GRGIDLHLFPENVEFKGDCRTSGFRCPEMLENKPWKYQVDIYGFCVVVHTMLHGSYMEVE 480
           GRGID+HLFP+N+EFKGDCRTSGFRC EM EN+PW +QVD YG CVVVH MLH SYME+E
Sbjct: 421 GRGIDMHLFPDNMEFKGDCRTSGFRCVEMQENRPWTFQVDTYGLCVVVHMMLHNSYMEIE 480

Query: 481 KKASSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPGDYNKELLESIRKSFQEYMCSN 531
           KK S +G ++Y PKSSFKRYWN ELW+NLF KLLN +PG  +K+LL+ +R++FQ+YMCS+
Sbjct: 481 KKLSPEGGYIYQPKSSFKRYWNGELWRNLFKKLLNSSPGGNDKKLLQDLRETFQDYMCSD 540

BLAST of Cla002231 vs. TrEMBL
Match: K7LU99_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_12G116400 PE=4 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 2.4e-206
Identity = 348/514 (67.70%), Postives = 413/514 (80.35%), Query Frame = 1

Query: 19  DPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNVKYKNDVRFLKIWFLSIGM 78
           DPLLP+L SIK ALE S     S   L+NLL DCI NF  N +Y+NDVRFLKIW L +G+
Sbjct: 21  DPLLPFLRSIKKALEASDD---SASNLSNLLKDCIRNFKNNDRYRNDVRFLKIWLLYMGV 80

Query: 79  REDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANIVYELGLMRNAEPLEWLKK 138
            +DFE+ FKE+L+  +C  +S LY+W A F E KGRL+DA  +Y+LG+ RNAEP+EWLKK
Sbjct: 81  SDDFESVFKEMLDSNVCTNSSSLYVWSASFFELKGRLHDALTIYQLGICRNAEPIEWLKK 140

Query: 139 AKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSIMNDLLSKINPKIMKYDGYH 198
           A  LF++RISE+ NA S Q  D  ES K  ++ INPW +S M+ LL KI P IMK+DGY 
Sbjct: 141 AHALFLNRISEIQNAASTQKVDDKESKKFEDNGINPWGTSTMDSLLKKIYPLIMKFDGYR 200

Query: 199 SSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVAL 258
           SSTK Y+ K+AL +L+NSSRNK+IEIGGKKY IKGCAG GGFAQVYK+ +DS+PD+VVAL
Sbjct: 201 SSTKPYTGKVALSTLKNSSRNKVIEIGGKKYHIKGCAGQGGFAQVYKANVDSDPDNVVAL 260

Query: 259 KIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINL 318
           KIQ P FPWEFY+YRQLD R+  ++RSS+G AH +++YSD SILICDYLA+GTLQD IN 
Sbjct: 261 KIQKPAFPWEFYIYRQLDKRILDRERSSYGFAHRIHIYSDCSILICDYLANGTLQDVINS 320

Query: 319 YVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDSLTEDEFQERS 378
           YVVLGK+MEEVLCIYYTIEML+M+ETLH  G+IHGDFKPDNLLIRYA   LTED F  R 
Sbjct: 321 YVVLGKSMEEVLCIYYTIEMLHMVETLHGFGLIHGDFKPDNLLIRYARGDLTEDGFFSRI 380

Query: 379 GPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEMLENKPWKYQVDIYGFCVV 438
           GPW DQGLCLVDWGRGIDLHLFP+++ FKGDCRTSGFRC EMLE+KPWK+QVD YG C +
Sbjct: 381 GPWCDQGLCLVDWGRGIDLHLFPDHILFKGDCRTSGFRCIEMLEDKPWKFQVDAYGLCAI 440

Query: 439 VHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPGDYNKELLE 498
           VH MLH SYMEV KK  SDG ++YLPK  FKRYWNIELWK  FTK+LN  P D ++ LL+
Sbjct: 441 VHMMLHSSYMEVVKKEQSDGSYMYLPKLPFKRYWNIELWKTFFTKMLNQYPHDDDRSLLQ 500

Query: 499 SIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
            ++KSFQ+YM SNPQLI+KLKELL KQRASLC+A
Sbjct: 501 DLKKSFQDYMSSNPQLIKKLKELLSKQRASLCSA 531

BLAST of Cla002231 vs. NCBI nr
Match: gi|659110537|ref|XP_008455277.1| (PREDICTED: mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucumis melo])

HSP 1 Score: 1019.6 bits (2635), Expect = 2.0e-294
Identity = 494/532 (92.86%), Postives = 509/532 (95.68%), Query Frame = 1

Query: 1   MAIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNV 60
           M I FADSQIS+TTASAQDPLLPWLWSIK ALENSTSGNVSG ELA LLSDCI NFSGNV
Sbjct: 1   MTISFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEIC+GNSLLYIWLA FLESKGRLNDANI
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120

Query: 121 VYELGLMRNAEPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSIM 180
           VYELGLMRNAEPLEWLKKAK LFIDRISELVN +SMQVND SESTK AESYINPWSSSIM
Sbjct: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180

Query: 181 NDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
           NDLL+KINPKIMKYDGYHSSTK YSKKM LKSLRNSSRNK IEIGGKKYEIKGCAGTGGF
Sbjct: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRV  K+RSSFGCAH MYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           ILICDYLA+GTLQDAIN YVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL
Sbjct: 301 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360

Query: 361 LIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEM 420
           L+RYAG++LTED+FQER GPWQDQGLCLVDWGRGIDL LFPENVEFKGDCRTSGFRCPEM
Sbjct: 361 LVRYAGENLTEDDFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LEN+PWKYQVDIYG CVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480

Query: 481 FTKLLNITPGDYNKELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           FTKLLNITPG YNKELL+S+RKSFQ+YMCSNPQLIRKLKELLVKQRASLC +
Sbjct: 481 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 532

BLAST of Cla002231 vs. NCBI nr
Match: gi|449438133|ref|XP_004136844.1| (PREDICTED: mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucumis sativus])

HSP 1 Score: 1013.8 bits (2620), Expect = 1.1e-292
Identity = 493/532 (92.67%), Postives = 506/532 (95.11%), Query Frame = 1

Query: 1   MAIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNV 60
           MAIFFADSQISTTTAS QDPLLPWLWSIK ALENSTSGNVSG ELA LLSDCI NF GNV
Sbjct: 1   MAIFFADSQISTTTASPQDPLLPWLWSIKAALENSTSGNVSGSELAKLLSDCIGNFRGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEIC+ NSLLYIWLA FLESKGRLNDANI
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICISNSLLYIWLAAFLESKGRLNDANI 120

Query: 121 VYELGLMRNAEPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSIM 180
           VYELGLMRNAEPLEWLKKAK LFIDRISELVN +SMQVND SESTK AESYINPWSSSIM
Sbjct: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180

Query: 181 NDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
           +DLL+KI+PKIMKYDGYHSSTK YSKKM LKSLRNSSRNK IEIGGKKYEIKGCAGTGGF
Sbjct: 181 SDLLNKISPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRV  K+RSSFGCAH MYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAIN YVVLGKTMEEVLCIYYTIEMLYMLETLHSI IIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIDIIHGDFKPDNL 360

Query: 361 LIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEM 420
           LIRYAG+S+TED FQER GPWQDQGLCLVDWGRGIDL LFPENVEFKGDCRTSGFRCPEM
Sbjct: 361 LIRYAGESVTEDGFQERHGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LEN+PWKYQVDIYG CVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480

Query: 481 FTKLLNITPGDYNKELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           FTKLLNITPG YNKELL+S+RKSFQ+YMCSNPQLIRKLKELLVKQRASLC +
Sbjct: 481 FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 532

BLAST of Cla002231 vs. NCBI nr
Match: gi|1009177590|ref|XP_015870056.1| (PREDICTED: mitotic checkpoint serine/threonine-protein kinase BUB1, partial [Ziziphus jujuba])

HSP 1 Score: 759.2 bits (1959), Expect = 4.8e-216
Identity = 353/494 (71.46%), Postives = 419/494 (84.82%), Query Frame = 1

Query: 38  GNVSGPELANLLSDCIENFSGNVKYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICVG 97
           G  SG +L+ LLSDCI  F  N +Y+ND+RFLKIWFL IG  EDF T F ++LE +ICVG
Sbjct: 1   GKDSGLDLSKLLSDCITTFKNNGQYRNDIRFLKIWFLHIGFSEDFGTIFTDMLESKICVG 60

Query: 98  NSLLYIWLAVFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNANSMQ 157
           +S+LYIW A FLE+KG+L DA +VY++G+MRNAEP+E +KKA+ LF+DR+S+++NA+  +
Sbjct: 61  HSVLYIWYASFLEAKGKLFDAQMVYQMGIMRNAEPVEMMKKAQALFLDRMSDIINASPPK 120

Query: 158 VNDGSESTKLAESYINPWSSSIMNDLLSKINPKIMKYDGYHSSTKVYSKKMALKSLRNSS 217
            ND  ES +    +INPWSSS++ DLL K+NP+I KYDGYHSS K Y  K+AL SL+NSS
Sbjct: 121 KNDDDESIRSGNCHINPWSSSVIKDLLKKMNPQITKYDGYHSSAKAYPGKVALSSLKNSS 180

Query: 218 RNKIIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDL 277
           RNKIIEIGGKKY+IKGCAG GGFAQVYK+Y++SNPD+VVALKIQ P FPWEFYMYRQLD 
Sbjct: 181 RNKIIEIGGKKYQIKGCAGQGGFAQVYKAYVNSNPDEVVALKIQKPAFPWEFYMYRQLDQ 240

Query: 278 RVTGKQRSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIE 337
           R+  K+RSSFG AH M+LYSD SILICDYLAHGTLQDAIN YVV+ K MEEVLCIYYTIE
Sbjct: 241 RIPAKERSSFGFAHRMHLYSDCSILICDYLAHGTLQDAINAYVVVAKPMEEVLCIYYTIE 300

Query: 338 MLYMLETLHSIGIIHGDFKPDNLLIRYAGDSLTEDEFQERSGPWQDQGLCLVDWGRGIDL 397
           ML+MLETLH  GIIHGDFKPDNL+IRYA D L ED F+ERSGPW DQGL L+DWGRGIDL
Sbjct: 301 MLHMLETLHGAGIIHGDFKPDNLIIRYARDDLVEDGFRERSGPWLDQGLYLIDWGRGIDL 360

Query: 398 HLFPENVEFKGDCRTSGFRCPEMLENKPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSD 457
           HLFP+N EF GDCRTSGFRC EM +NKPWK+QVD YG CV+ H MLHGSYME+E+KAS+D
Sbjct: 361 HLFPDNAEFNGDCRTSGFRCIEMQQNKPWKFQVDTYGLCVIAHMMLHGSYMEIEEKASTD 420

Query: 458 GDFVYLPKSSFKRYWNIELWKNLFTKLLNITPGDYNKELLESIRKSFQEYMCSNPQLIRK 517
           G ++YLPK+SFKRYWN++LWKNLFTKLLN    D +KELL+++R+SFQ+YMCSNPQLIRK
Sbjct: 421 GGYIYLPKTSFKRYWNVDLWKNLFTKLLNNNHSDKDKELLQNLRESFQDYMCSNPQLIRK 480

Query: 518 LKELLVKQRASLCN 532
           LKELL KQRASLC+
Sbjct: 481 LKELLAKQRASLCS 494

BLAST of Cla002231 vs. NCBI nr
Match: gi|359485399|ref|XP_002274770.2| (PREDICTED: mitotic checkpoint serine/threonine-protein kinase BUB1 [Vitis vinifera])

HSP 1 Score: 755.4 bits (1949), Expect = 6.9e-215
Identity = 358/522 (68.58%), Postives = 428/522 (81.99%), Query Frame = 1

Query: 11  STTTASAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNVKYKNDVRFLK 70
           ST  AS  DPLLPWLWSIK AL+   S + +G +L NLLS+CI  F GN +Y+ND RFLK
Sbjct: 8   STNDASGNDPLLPWLWSIKKALDGFNSSHKNGKDLENLLSNCITTFKGNPQYRNDPRFLK 67

Query: 71  IWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANIVYELGLMRNA 130
           IWFL +  REDF + F+E+ E +ICVG SLLY   A+FLE+KG+L DA +VY++G+ R A
Sbjct: 68  IWFLYLDAREDFASIFREMEENKICVGQSLLYESYALFLEAKGKLLDAFMVYQIGVSRKA 127

Query: 131 EPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSIMNDLLSKINPK 190
           EPL+ LKKA+ LF+DR+ E+VNA S++  D  ES +L  S+ NPWS S + DLL K  P+
Sbjct: 128 EPLDKLKKAQALFMDRMKEIVNACSLEPIDDGESAELGTSFTNPWSISTIKDLLQKKKPQ 187

Query: 191 IMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGFAQVYKSYLDS 250
           I+KYDGYH STK YS+K+AL SL+NSSRNKIIEIGG KY+IKGCAG GGFAQV+K+Y++ 
Sbjct: 188 ILKYDGYHPSTKAYSRKVALSSLQNSSRNKIIEIGGYKYQIKGCAGKGGFAQVFKAYVNY 247

Query: 251 NPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYSILICDYLAHG 310
           NPD+VVALKIQ P FPWEFYMYRQLD R+  K+RSSFG AH ++LYSD SIL+ +YLAHG
Sbjct: 248 NPDEVVALKIQKPAFPWEFYMYRQLDKRIPDKERSSFGFAHRLHLYSDCSILVSEYLAHG 307

Query: 311 TLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDSLT 370
           TLQDAIN YVV+G +MEEVLCIYYTIEMLYMLET+HS+GIIHGDFKPDNLLIRY+   LT
Sbjct: 308 TLQDAINSYVVIGGSMEEVLCIYYTIEMLYMLETIHSVGIIHGDFKPDNLLIRYSRHDLT 367

Query: 371 EDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEMLENKPWKYQV 430
           E+EF ER GPW+DQGLCLVDWGRGIDL LFP N EFKGDCRTSGFRC EM E+KPWK+QV
Sbjct: 368 EEEFHERCGPWRDQGLCLVDWGRGIDLSLFPSNTEFKGDCRTSGFRCVEMQEHKPWKFQV 427

Query: 431 DIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPG 490
           D YG CV+VH MLH SYM +EKK S DG ++Y PKS FKRYWN++LWKNLFTKLLN +P 
Sbjct: 428 DTYGLCVIVHMMLHNSYMAIEKKPSFDGGYIYQPKSPFKRYWNVQLWKNLFTKLLNSSPH 487

Query: 491 DYNKELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           + +  LL+++R+SFQ+YMCSNPQLI+KLK LLVKQRASLC A
Sbjct: 488 EDHSRLLQNLRESFQDYMCSNPQLIKKLKHLLVKQRASLCCA 529

BLAST of Cla002231 vs. NCBI nr
Match: gi|590690881|ref|XP_007043630.1| (ATP binding,protein kinases,protein serine/threonine kinases isoform 1 [Theobroma cacao])

HSP 1 Score: 751.9 bits (1940), Expect = 7.6e-214
Identity = 353/522 (67.62%), Postives = 428/522 (81.99%), Query Frame = 1

Query: 11  STTTASAQDPLLPWLWSIKDALENSTSGNVSGPELANLLSDCIENFSGNVKYKNDVRFLK 70
           S  ++SA DPL PWL SIK AL+   SGN +G +L NLLSDCI  F  N KY+ND+RFLK
Sbjct: 8   SNDSSSAHDPLFPWLTSIKKALDEWYSGNRTGADLDNLLSDCISTFKHNAKYRNDLRFLK 67

Query: 71  IWFLSIGMREDFETGFKELLEQEICVGNSLLYIWLAVFLESKGRLNDANIVYELGLMRNA 130
           IWF+ +   +DFE+ F+E+ E EIC+G+SLLY W A FLE+KG+  +A+ VY++G+ R A
Sbjct: 68  IWFIYLEGSKDFESVFREIEENEICIGHSLLYEWYAYFLEAKGKWKEAHTVYQIGISRKA 127

Query: 131 EPLEWLKKAKTLFIDRISELVNANSMQVNDGSESTKLAESYINPWSSSIMNDLLSKINPK 190
           +PLE LK A++LF++R+S+ +N +S    DGSE  +  + +INPWS+  + +L  KI+P+
Sbjct: 128 DPLEKLKGAQSLFVERMSDRLNGSSFGKIDGSEPAEFGKKFINPWSTFTIEELSKKIHPQ 187

Query: 191 IMKYDGYHSSTKVYSKKMALKSLRNSSRNKIIEIGGKKYEIKGCAGTGGFAQVYKSYLDS 250
           I KYDGYH S KVYS K+AL SL+ SSRNKIIEIGGKKY+IKGCAG GGFAQV+K+Y+DS
Sbjct: 188 ITKYDGYHLSKKVYSGKVALSSLKKSSRNKIIEIGGKKYQIKGCAGQGGFAQVFKAYIDS 247

Query: 251 NPDDVVALKIQTPPFPWEFYMYRQLDLRVTGKQRSSFGCAHDMYLYSDYSILICDYLAHG 310
           NPDDVVALKIQ P FPWEFYMYRQLD R+  KQRS+FG A  +++YSD SIL+CDYL+HG
Sbjct: 248 NPDDVVALKIQKPAFPWEFYMYRQLDERILDKQRSNFGSAQKIHIYSDCSILVCDYLSHG 307

Query: 311 TLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDSLT 370
           TLQDAIN YVV GK+MEEVLCIYYT EMLYMLETLH +GIIHGDFKPDNLLIRY+ D L+
Sbjct: 308 TLQDAINSYVVTGKSMEEVLCIYYTTEMLYMLETLHRVGIIHGDFKPDNLLIRYSKDDLS 367

Query: 371 EDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEMLENKPWKYQV 430
           ED F++R+GPW DQGLCLVDWGRGIDLHLFP+N EF GDCRTSGFRC +M E KPW +QV
Sbjct: 368 EDGFKDRTGPWHDQGLCLVDWGRGIDLHLFPDNTEFTGDCRTSGFRCIQMQEKKPWTFQV 427

Query: 431 DIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPG 490
           D YG CVVVH MLH +YME+EKKAS D D++YLPKSSFKRYWN+ LWK+LFTKLLN++PG
Sbjct: 428 DTYGLCVVVHMMLHNTYMEIEKKASDD-DYIYLPKSSFKRYWNVNLWKDLFTKLLNVSPG 487

Query: 491 DYNKELLESIRKSFQEYMCSNPQLIRKLKELLVKQRASLCNA 533
           + + ELL+S+RKSF EYMC NP L+ KLKELLVKQRASLC A
Sbjct: 488 NNDAELLQSLRKSFLEYMCDNPPLVNKLKELLVKQRASLCCA 528