Cla97C02G027660 (gene) Watermelon (97103) v2

NameCla97C02G027660
Typegene
OrganismCitrullus lanatus (Watermelon (97103) v2)
DescriptionLipoyl synthase, mitochondrial
LocationCla97Chr02 : 1305206 .. 1307356 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAAACGTTGCCAAAGCCTTGCTCGAATCCTCAAATCCGTCAGTAGATCCTTCTCTTCTTCTTCTTCCTCCACCTCCGCCTCTGCTACCTCCTCTCAATTCCCCCGCACTTTGGCCGGTCTCCGGGAACGACTCGCCGCCGAGTCCCCCTCACTTTCCGACTTCGTTGACCTGCAATCTTCTGATTCCTATTCTGTGGAGGTTGGTACGAAGAAGAAGCCCCTTCCTAAACCTAAATGGATGAAGGAATCGGTGCCTGGTGGCGAGAAGTATGTTCAAATCAAGAAAAAGCTCCGCGAATTGAAGCTTCATACGGTTTGTGAGGAGGCTAAATGCCCTAATCTAGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACGATTATGATTCTTGGTGACACTTGTACCCGTGGTTGCAGGTTAGTAGCAATTCAGTTTATTCGTTCTCGTTATGTTTACTTAATTGTCAATGTGTTTTTGACGATTGAAGAGTGGAGAACCATAATTTCTGGTTATTGTCCTGTGTATAATGTCAACTTATATATACTGGATTTAGAACTGATTGCCTTTCTCGTAGAAATAAGTGTCGATTACTTTGATATCAACTCAGTGCCTCAGAATTTCTATTAATTCCATTGCTGTTTTGGGATTGTTTGAACTCCAGGTTCTGTAACGTGAAGACTTCACGAACTCCACCTCCACCGGACCCAAATGAGCCGACAAATGTCGCTGAGGCAATCGCATCATGGGGTTTGGATTATGTAGTCATCACTAGTGTTGATCGCGACGATTTACCTGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGAAGAAACTTAAGGTGTTGAAGCCGAATATGCTGATTGAGGCATTGGGTACTAGTTCCCTGGTTTCTATTACGTATAACAGTGTCAAGTTCATTGTTGCAATAAAGGATCTTTGATGTGACGCTTGATTTTAAAGAGCCTTATTTTATTTGGTTTCGATGCAACTAAGGAATTTAGCGAAAGCGACTTATTTTACCCTGTACTACTGATGTCGCAAAAATCAGCATCATATGCTGATTTGCTTCTGAATATGTTTGTTCAGTGTGATTTAATCTGTTCTTTGATGTTGCTAATGCTTAACTAATTGATTCTGCAGTTCCTGATTTTCGAGGAGACCCTGGCTGTGTAGAGAAAGTTGCAAAGTCAGGCCTTGACGTCTTTGCTCACAATATAGAGACAGTTGAAGAGCTTCAGGGGGCAGTTAGGGATCACCGTGCTAATTTCAAGCAATCATTGGATGTTCTAGTTATGGCCAAAGGGTTTGCTCCTCCTGGAACACTTACGAAGACTTCCATTATGTTAGGCTGTGGAGAAACACCAGATCAAGTCGTGCAAACAATGGAGAAGGTGAGAGCAGCTGGAGTTGATGTGATGACGTTTGGTCAGTATATGAGACCTTCAAAGCGCCATATGCCTGTATCGGAATACATTACTCCTGAGGCTTTCGAGAAGTATCAAAATCTTGGCATGAAAATGGTACTCTCTCTTATTATTTGAACATTTTGATCGTGTACTGTCTTCTTTGTTATGCAAGGGATTGATTAATCGAGTTCTTGAAGGCTAAAGCGTACTTGATTTGACTTTTCTTTTCTTTTCTTTTTCCTTATTTTAATGTCTAGTATTGTATCATTTCCCCCATCTTTTCTAGGACTGATCAACTTACTAAGTGCTTAATTTCTTTTTCACTTAAAAAACTTTTTCAAGCACACTTAAAAAGTCAATCCAAACCGGTCCTATTGGATTTGTACCATTGTAATTTTATGTGTTTGAATCGTTCAAGGGAGTCCATGTTTTACTTTAAAACGTATACTAGTTCACACTGATGCATAACCCTCATTTGTAAGATTATAGGAAATCTCCACCATCATGTATCATATGATCAATAGTTTGATAATGGATGGAGTTACTTGCCATGCCTTGAGGGTGCAACAATTATCTAAAAACCATTTTTTTGTTTGGTTGTGGCAGGGTTTCCGGTATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAGGCAGGAGAATTCTACATCAAGTCAATGATTGAATCGGATCGGGCAGCTTCTAATTTACAGCCTACACAACCTTGA

mRNA sequence

ATGAACAAACGTTGCCAAAGCCTTGCTCGAATCCTCAAATCCGTCAGTAGATCCTTCTCTTCTTCTTCTTCCTCCACCTCCGCCTCTGCTACCTCCTCTCAATTCCCCCGCACTTTGGCCGGTCTCCGGGAACGACTCGCCGCCGAGTCCCCCTCACTTTCCGACTTCGTTGACCTGCAATCTTCTGATTCCTATTCTGTGGAGGTTGGTACGAAGAAGAAGCCCCTTCCTAAACCTAAATGGATGAAGGAATCGGTGCCTGGTGGCGAGAAGTATGTTCAAATCAAGAAAAAGCTCCGCGAATTGAAGCTTCATACGGTTTGTGAGGAGGCTAAATGCCCTAATCTAGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACGATTATGATTCTTGGTGACACTTGTACCCGTGGTTGCAGGTTCTGTAACGTGAAGACTTCACGAACTCCACCTCCACCGGACCCAAATGAGCCGACAAATGTCGCTGAGGCAATCGCATCATGGGGTTTGGATTATGTAGTCATCACTAGTGTTGATCGCGACGATTTACCTGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGAAGAAACTTAAGGTGTTGAAGCCGAATATGCTGATTGAGGCATTGGTTCCTGATTTTCGAGGAGACCCTGGCTGTGTAGAGAAAGTTGCAAAGTCAGGCCTTGACGTCTTTGCTCACAATATAGAGACAGTTGAAGAGCTTCAGGGGGCAGTTAGGGATCACCGTGCTAATTTCAAGCAATCATTGGATGTTCTAGTTATGGCCAAAGGGTTTGCTCCTCCTGGAACACTTACGAAGACTTCCATTATGTTAGGCTGTGGAGAAACACCAGATCAAGTCGTGCAAACAATGGAGAAGGTGAGAGCAGCTGGAGTTGATGTGATGACGTTTGGTCAGTATATGAGACCTTCAAAGCGCCATATGCCTGTATCGGAATACATTACTCCTGAGGCTTTCGAGAAGTATCAAAATCTTGGCATGAAAATGGGTTTCCGGTATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAGGCAGGAGAATTCTACATCAAGTCAATGATTGAATCGGATCGGGCAGCTTCTAATTTACAGCCTACACAACCTTGA

Coding sequence (CDS)

ATGAACAAACGTTGCCAAAGCCTTGCTCGAATCCTCAAATCCGTCAGTAGATCCTTCTCTTCTTCTTCTTCCTCCACCTCCGCCTCTGCTACCTCCTCTCAATTCCCCCGCACTTTGGCCGGTCTCCGGGAACGACTCGCCGCCGAGTCCCCCTCACTTTCCGACTTCGTTGACCTGCAATCTTCTGATTCCTATTCTGTGGAGGTTGGTACGAAGAAGAAGCCCCTTCCTAAACCTAAATGGATGAAGGAATCGGTGCCTGGTGGCGAGAAGTATGTTCAAATCAAGAAAAAGCTCCGCGAATTGAAGCTTCATACGGTTTGTGAGGAGGCTAAATGCCCTAATCTAGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACGATTATGATTCTTGGTGACACTTGTACCCGTGGTTGCAGGTTCTGTAACGTGAAGACTTCACGAACTCCACCTCCACCGGACCCAAATGAGCCGACAAATGTCGCTGAGGCAATCGCATCATGGGGTTTGGATTATGTAGTCATCACTAGTGTTGATCGCGACGATTTACCTGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGAAGAAACTTAAGGTGTTGAAGCCGAATATGCTGATTGAGGCATTGGTTCCTGATTTTCGAGGAGACCCTGGCTGTGTAGAGAAAGTTGCAAAGTCAGGCCTTGACGTCTTTGCTCACAATATAGAGACAGTTGAAGAGCTTCAGGGGGCAGTTAGGGATCACCGTGCTAATTTCAAGCAATCATTGGATGTTCTAGTTATGGCCAAAGGGTTTGCTCCTCCTGGAACACTTACGAAGACTTCCATTATGTTAGGCTGTGGAGAAACACCAGATCAAGTCGTGCAAACAATGGAGAAGGTGAGAGCAGCTGGAGTTGATGTGATGACGTTTGGTCAGTATATGAGACCTTCAAAGCGCCATATGCCTGTATCGGAATACATTACTCCTGAGGCTTTCGAGAAGTATCAAAATCTTGGCATGAAAATGGGTTTCCGGTATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAGGCAGGAGAATTCTACATCAAGTCAATGATTGAATCGGATCGGGCAGCTTCTAATTTACAGCCTACACAACCTTGA

Protein sequence

MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQSSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVKKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKGFAPPGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAASNLQPTQP
BLAST of Cla97C02G027660 vs. NCBI nr
Match: XP_016901725.1 (PREDICTED: lipoyl synthase 2, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 711.8 bits (1836), Expect = 1.2e-201
Identity = 373/380 (98.16%), Postives = 376/380 (98.95%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVXXXXXXXXXXXXXXXXXXQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           MNKRCQSLARILKSVXXXXXXXXXXXXXXXXXXQFPRTLAGLRERLAAESPSLSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVXXXXXXXXXXXXXXXXXXQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVKKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETV+KLKVLKPNMLIEALVPDFRGD GCVEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKGFAPPGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQGAVRDHRANFKQSLDVLVMAK F+P GTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNLQPTQ 381
           FYIKSMIESDRAASN QPTQ
Sbjct: 361 FYIKSMIESDRAASNSQPTQ 380

BLAST of Cla97C02G027660 vs. NCBI nr
Match: XP_004136815.1 (PREDICTED: lipoyl synthase 2, mitochondrial [Cucumis sativus] >KGN43590.1 hypothetical protein Csa_7G047260 [Cucumis sativus])

HSP 1 Score: 702.2 bits (1811), Expect = 9.6e-199
Identity = 368/380 (96.84%), Postives = 373/380 (98.16%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVXXXXXXXXXXXXXXXXXXQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           MNKRCQSLARILKSVXXXXXXXXXXXXXXXXXXQFPRTLAGLRERLAAESP LSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVXXXXXXXXXXXXXXXXXXQFPRTLAGLRERLAAESPILSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWM+ESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVKKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETV+KLKVLKPNMLIEALVPDFRGD GCVEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKGFAPPGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQG VRDHRANFKQSLDVLVMAK F+  GTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQN+GMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNLQPTQ 381
           FYIKSMIESDRAASN QPTQ
Sbjct: 361 FYIKSMIESDRAASNSQPTQ 380

BLAST of Cla97C02G027660 vs. NCBI nr
Match: XP_022988813.1 (lipoyl synthase 2, mitochondrial [Cucurbita maxima])

HSP 1 Score: 688.7 bits (1776), Expect = 1.1e-194
Identity = 354/381 (92.91%), Postives = 358/381 (93.96%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVXXXXXXXXXXXXXXXXXXQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           MN R  SLARI +S+        XXXXXXXXX Q PRTLAGLRERLAAESP+LSDFV L+
Sbjct: 1   MNTRRHSLARIFESIGRSLSYSSXXXXXXXXXSQVPRTLAGLRERLAAESPTLSDFVSLK 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPQNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVKKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETV KLKVLKPNMLIEALVPDFRGDPG VEKVAKSGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGSVEKVAKSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKGFAPPGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQGAVRDHRANFKQSLDVLVMAK FAP GTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKY NLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNLQPTQP 382
           FYIKSMIESDRAASN  PTQP
Sbjct: 361 FYIKSMIESDRAASNSHPTQP 381

BLAST of Cla97C02G027660 vs. NCBI nr
Match: XP_023531015.1 (lipoyl synthase 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 687.6 bits (1773), Expect = 2.5e-194
Identity = 353/380 (92.89%), Postives = 357/380 (93.95%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVXXXXXXXXXXXXXXXXXXQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           MN R  SLARI +SV        XXXXXXXX  Q PRTLAGLRERLAAESP+LSDFV L+
Sbjct: 1   MNTRRHSLARIFESVGRSLSYSSXXXXXXXXSSQIPRTLAGLRERLAAESPTLSDFVGLK 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPQNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVKKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETV+KLKVLKPNMLIEALVPDFRGDPG VEKVAKSGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGSVEKVAKSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKGFAPPGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQGAVRDHRANFKQSLDVLVMAK FAP GTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKY NLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNLQPTQ 381
           FYIKSMIESDRAASN  PTQ
Sbjct: 361 FYIKSMIESDRAASNSHPTQ 380

BLAST of Cla97C02G027660 vs. NCBI nr
Match: XP_022147922.1 (lipoyl synthase 2, mitochondrial [Momordica charantia])

HSP 1 Score: 686.8 bits (1771), Expect = 4.2e-194
Identity = 358/380 (94.21%), Postives = 361/380 (95.00%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVXXXXXXXXXXXXXXXXXXQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M   CQSL RIL+S     XXXXXXXXXXXXXXQFPRTLA LRERLAAESPSLSDFV LQ
Sbjct: 1   MKTPCQSLVRILESFGRSFXXXXXXXXXXXXXXQFPRTLAALRERLAAESPSLSDFVGLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S+DSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  STDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVKKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETV+ LKVLKPNMLIEALVPDFRGD GCVEKVAKSGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQTLKVLKPNMLIEALVPDFRGDIGCVEKVAKSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKGFAPPGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQGAVRDHRANFKQSLDVL MAK FAP GTLTKTSIMLGCGE PDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLAMAKDFAPSGTLTKTSIMLGCGEKPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNLQPTQ 381
           FYIKSMIESDRAASN QPTQ
Sbjct: 361 FYIKSMIESDRAASNSQPTQ 380

BLAST of Cla97C02G027660 vs. TrEMBL
Match: tr|A0A1S4E162|A0A1S4E162_CUCME (Lipoyl synthase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495529 PE=3 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 8.0e-202
Identity = 373/380 (98.16%), Postives = 376/380 (98.95%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVXXXXXXXXXXXXXXXXXXQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           MNKRCQSLARILKSVXXXXXXXXXXXXXXXXXXQFPRTLAGLRERLAAESPSLSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVXXXXXXXXXXXXXXXXXXQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVKKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETV+KLKVLKPNMLIEALVPDFRGD GCVEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKGFAPPGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQGAVRDHRANFKQSLDVLVMAK F+P GTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNLQPTQ 381
           FYIKSMIESDRAASN QPTQ
Sbjct: 361 FYIKSMIESDRAASNSQPTQ 380

BLAST of Cla97C02G027660 vs. TrEMBL
Match: tr|A0A0A0K1R3|A0A0A0K1R3_CUCSA (Lipoyl synthase, mitochondrial OS=Cucumis sativus OX=3659 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 6.4e-199
Identity = 368/380 (96.84%), Postives = 373/380 (98.16%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVXXXXXXXXXXXXXXXXXXQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           MNKRCQSLARILKSVXXXXXXXXXXXXXXXXXXQFPRTLAGLRERLAAESP LSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVXXXXXXXXXXXXXXXXXXQFPRTLAGLRERLAAESPILSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWM+ESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVKKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETV+KLKVLKPNMLIEALVPDFRGD GCVEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKGFAPPGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQG VRDHRANFKQSLDVLVMAK F+  GTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQN+GMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNLQPTQ 381
           FYIKSMIESDRAASN QPTQ
Sbjct: 361 FYIKSMIESDRAASNSQPTQ 380

BLAST of Cla97C02G027660 vs. TrEMBL
Match: tr|A0A1R3J2J1|A0A1R3J2J1_COCAP (Lipoyl synthase, mitochondrial OS=Corchorus capsularis OX=210143 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 2.6e-184
Identity = 323/375 (86.13%), Postives = 339/375 (90.40%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVXXXXXXXXXXXXXXXXXXQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M  R  SLAR LKS                   +FP TLAGLR RLAAESP+LSDFV LQ
Sbjct: 2   MQSRFSSLARTLKSSAAKSRRFSSTVESPGTKPEFPPTLAGLRARLAAESPALSDFVGLQ 61

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           + + YSVEVGTKKKPLPKPKWM+ES+PGGEKYVQIKKKL+ELKLHTVCEEA+CPNLGECW
Sbjct: 62  NDNQYSVEVGTKKKPLPKPKWMRESIPGGEKYVQIKKKLKELKLHTVCEEARCPNLGECW 121

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVVIT
Sbjct: 122 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPGNVAEAIASWGLDYVVIT 181

Query: 181 SVDRDDLPDQGSGHFAETVKKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETV+KLK LKPNMLIEALVPDFRGD GCVEKVAKSGLDVFAHNI
Sbjct: 182 SVDRDDLPDQGSGHFAETVQKLKALKPNMLIEALVPDFRGDAGCVEKVAKSGLDVFAHNI 241

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKGFAPPGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ AVRDHRANFKQSLDVL+MAK +AP GTLTKTSIMLGCGETPDQV++TMEKVR
Sbjct: 242 ETVEELQRAVRDHRANFKQSLDVLMMAKDYAPAGTLTKTSIMLGCGETPDQVIKTMEKVR 301

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 302 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGE 361

Query: 361 FYIKSMIESDRAASN 376
           FYIKSMIESDRAA++
Sbjct: 362 FYIKSMIESDRAAAS 376

BLAST of Cla97C02G027660 vs. TrEMBL
Match: tr|A0A1R3JV88|A0A1R3JV88_9ROSI (Lipoyl synthase, mitochondrial OS=Corchorus olitorius OX=93759 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 1.7e-183
Identity = 321/375 (85.60%), Postives = 339/375 (90.40%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVXXXXXXXXXXXXXXXXXXQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M  R  SLAR LKS                   +FP TLAGLR RLAAE+P+LSDFV LQ
Sbjct: 2   MQSRFSSLARTLKSSAAKSRRFSSTVESPGTKPEFPPTLAGLRARLAAEAPALSDFVGLQ 61

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           + + YSVEVGTKKKPLPKPKWM+ES+PGGEKYVQIKKKL+ELKLHTVCEEA+CPNLGECW
Sbjct: 62  NDNQYSVEVGTKKKPLPKPKWMRESIPGGEKYVQIKKKLKELKLHTVCEEARCPNLGECW 121

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVVIT
Sbjct: 122 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPGNVAEAIASWGLDYVVIT 181

Query: 181 SVDRDDLPDQGSGHFAETVKKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETV+KLK LKPNMLIEALVPDFRGD GCVEKVAKSGLDVFAHNI
Sbjct: 182 SVDRDDLPDQGSGHFAETVQKLKALKPNMLIEALVPDFRGDAGCVEKVAKSGLDVFAHNI 241

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKGFAPPGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ AVRDHRANFKQSLDVL+MAK +AP GTLTKTSIMLGCGETP+QV++TMEKVR
Sbjct: 242 ETVEELQRAVRDHRANFKQSLDVLMMAKDYAPAGTLTKTSIMLGCGETPEQVIKTMEKVR 301

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 302 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGE 361

Query: 361 FYIKSMIESDRAASN 376
           FYIKSMIESDRAA++
Sbjct: 362 FYIKSMIESDRAAAS 376

BLAST of Cla97C02G027660 vs. TrEMBL
Match: tr|A0A1U8BEC4|A0A1U8BEC4_NELNU (Lipoyl synthase, mitochondrial OS=Nelumbo nucifera OX=4432 GN=LOC104612573 PE=3 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 1.6e-181
Identity = 310/344 (90.12%), Postives = 329/344 (95.64%), Query Frame = 0

Query: 34  QFPRTLAGLRERLAAESPSLSDFVDLQSSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYV 93
           +FP+TLAGLRERLA ESPSLSDF+ LQ +D YSVEVGTKK PLPKPKWMKES+PGG KY 
Sbjct: 62  EFPQTLAGLRERLAVESPSLSDFIHLQENDEYSVEVGTKKNPLPKPKWMKESIPGGSKYA 121

Query: 94  QIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRT 153
           +IKKKLRELKLHTVCEEA+CPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRT
Sbjct: 122 KIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRT 181

Query: 154 PPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVKKLKVLKPNMLIEA 213
           PPPPDPNEP+NVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAE V+KLK LKP+MLIEA
Sbjct: 182 PPPPDPNEPSNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAEAVQKLKALKPHMLIEA 241

Query: 214 LVPDFRGDPGCVEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKGFAPP 273
           LVPDFRGDPGCVEKVAKSGLDVFAHNIETVEELQ AVRDHRANF QS++VL MAKG+AP 
Sbjct: 242 LVPDFRGDPGCVEKVAKSGLDVFAHNIETVEELQRAVRDHRANFNQSIEVLKMAKGYAPS 301

Query: 274 GTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEK 333
           GTLTKTSIMLGCGETPDQVV+TMEKVRAAGVDVMTFGQYMRPSKRHMPVSEY+TPEAFEK
Sbjct: 302 GTLTKTSIMLGCGETPDQVVKTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYVTPEAFEK 361

Query: 334 YQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAASNLQ 378
           Y+ LGM+MGFRYVASGPMVRSSYKAGEFYIKSMIE+DRAAS+ Q
Sbjct: 362 YRTLGMEMGFRYVASGPMVRSSYKAGEFYIKSMIEADRAASSSQ 405

BLAST of Cla97C02G027660 vs. Swiss-Prot
Match: sp|B9H5L9|LIAS_POPTR (Lipoyl synthase, mitochondrial OS=Populus trichocarpa OX=3694 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 3.9e-183
Identity = 311/345 (90.14%), Postives = 330/345 (95.65%), Query Frame = 0

Query: 34  QFPRTLAGLRERLAAESPSLSDFVDLQSSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYV 93
           QF +TLAGLR RLA ESP+LSDF+ LQS+++YSVEVGTKKKPLPKPKWM+E++PGGEKYV
Sbjct: 39  QFSQTLAGLRARLAVESPTLSDFIHLQSNNTYSVEVGTKKKPLPKPKWMREAIPGGEKYV 98

Query: 94  QIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRT 153
           QIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRT
Sbjct: 99  QIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRT 158

Query: 154 PPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVKKLKVLKPNMLIEA 213
           PPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDL DQGSGHFAETV KLK LKPNMLIEA
Sbjct: 159 PPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLADQGSGHFAETVHKLKTLKPNMLIEA 218

Query: 214 LVPDFRGDPGCVEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKGFAPP 273
           LVPDFRGD GCVEKVAKSGLDVFAHNIETVEELQ +VRDHRANFKQSLDVL+MAK +APP
Sbjct: 219 LVPDFRGDRGCVEKVAKSGLDVFAHNIETVEELQSSVRDHRANFKQSLDVLMMAKEYAPP 278

Query: 274 GTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEK 333
           GTLTKTSIMLGCGE P+QVV+TMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITP+AFEK
Sbjct: 279 GTLTKTSIMLGCGEAPEQVVKTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPDAFEK 338

Query: 334 YQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAASNLQP 379
           Y+ LGM+MGFRYVASGPMVRSSYKAGEFYIKSMIESDR+ S+  P
Sbjct: 339 YKTLGMEMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSVSSQLP 383

BLAST of Cla97C02G027660 vs. Swiss-Prot
Match: sp|Q3LSN5|LIAS2_PEA (Lipoyl synthase 2, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-2 PE=2 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 3.5e-176
Identity = 302/342 (88.30%), Postives = 322/342 (94.15%), Query Frame = 0

Query: 34  QFPRTLAGLRERLAAESPSLSDFVDLQSSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYV 93
           +  + LA LR RLA ESPSLSDF+ L+S ++YSVEVGTKKKPLPKPKWMKES+PGGEKYV
Sbjct: 35  ELQQNLAALRARLAMESPSLSDFISLKSDNAYSVEVGTKKKPLPKPKWMKESIPGGEKYV 94

Query: 94  QIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRT 153
           QIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRT
Sbjct: 95  QIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRT 154

Query: 154 PPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVKKLKVLKPNMLIEA 213
           PP    +EPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHF ETV+KLK LKP+MLIEA
Sbjct: 155 PPXXXXDEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFTETVQKLKALKPSMLIEA 214

Query: 214 LVPDFRGDPGCVEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKGFAPP 273
           LVPDFRG+  CVEKV+KSGLDVFAHNIETVEELQ AVRDHRANFKQSLDVL+MAK +AP 
Sbjct: 215 LVPDFRGNAECVEKVSKSGLDVFAHNIETVEELQSAVRDHRANFKQSLDVLMMAKEYAPA 274

Query: 274 GTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEK 333
           GTLTKTSIMLGCGETPDQ+V+TMEKVRAAGVDVMTFGQ+MRPSKRHMPVSEYITPEAFEK
Sbjct: 275 GTLTKTSIMLGCGETPDQIVKTMEKVRAAGVDVMTFGQHMRPSKRHMPVSEYITPEAFEK 334

Query: 334 YQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAASN 376
           YQ LGM+MGFRYVASGPMVRSSYKAGEFYIKSMI+SDRAAS+
Sbjct: 335 YQTLGMEMGFRYVASGPMVRSSYKAGEFYIKSMIDSDRAASS 376

BLAST of Cla97C02G027660 vs. Swiss-Prot
Match: sp|Q3LSN4|LIAS1_PEA (Lipoyl synthase 1, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-1 PE=2 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 4.6e-176
Identity = 302/342 (88.30%), Postives = 321/342 (93.86%), Query Frame = 0

Query: 34  QFPRTLAGLRERLAAESPSLSDFVDLQSSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYV 93
           +  + LA LR RLA ESPSLSDF+ L+S ++YSVEVGTKKKPLPKPKWMKES+PGGEKYV
Sbjct: 35  ELQQNLAALRARLAMESPSLSDFISLKSDNAYSVEVGTKKKPLPKPKWMKESIPGGEKYV 94

Query: 94  QIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRT 153
           QIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRT
Sbjct: 95  QIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRT 154

Query: 154 PPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVKKLKVLKPNMLIEA 213
           PP    +EPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHF ETV+KLK LKP+ LIEA
Sbjct: 155 PPXXXXDEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFTETVQKLKALKPSTLIEA 214

Query: 214 LVPDFRGDPGCVEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKGFAPP 273
           LVPDFRG+  CVEKV+KSGLDVFAHNIETVEELQ AVRDHRANFKQSLDVL+MAK +AP 
Sbjct: 215 LVPDFRGNAECVEKVSKSGLDVFAHNIETVEELQSAVRDHRANFKQSLDVLMMAKEYAPA 274

Query: 274 GTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEK 333
           GTLTKTSIMLGCGETPDQ+V+TMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEK
Sbjct: 275 GTLTKTSIMLGCGETPDQIVKTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEK 334

Query: 334 YQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAASN 376
           YQ LGM+MGFRYVASGPMVRSSYKAGEFYIKSMI+SDRAAS+
Sbjct: 335 YQTLGMEMGFRYVASGPMVRSSYKAGEFYIKSMIDSDRAASS 376

BLAST of Cla97C02G027660 vs. Swiss-Prot
Match: sp|B7FM45|LIAS_MEDTR (Lipoyl synthase, mitochondrial OS=Medicago truncatula OX=3880 GN=LIP1 PE=2 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 8.6e-175
Identity = 311/375 (82.93%), Postives = 337/375 (89.87%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVXXXXXXXXXXXXXXXXXXQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M  R +++A+ LKS         XXXXXXXX  +FP+ L  LR RLA ESPSLSDF+ L+
Sbjct: 2   MYSRFRTVAKNLKSTTKPFSFTTXXXXXXXXSSEFPQNLTELRARLARESPSLSDFISLK 61

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S+++YSVEVGTKK PLPKPKWMKES+PGG KYVQIKKKLRELKLHTVCEEAKCPN+GECW
Sbjct: 62  SNNAYSVEVGTKKNPLPKPKWMKESIPGGWKYVQIKKKLRELKLHTVCEEAKCPNMGECW 121

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPP   P+EPTNVAEAIASWGLDYVVIT
Sbjct: 122 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPXXXPDEPTNVAEAIASWGLDYVVIT 181

Query: 181 SVDRDDLPDQGSGHFAETVKKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 240
           SV RDDLPDQGS HF ETV+KLK+LKP++LIEALVPDFRG+  CVEKV+KSGLDVFAHNI
Sbjct: 182 SVGRDDLPDQGSSHFTETVQKLKILKPSILIEALVPDFRGNAECVEKVSKSGLDVFAHNI 241

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKGFAPPGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ AVRDHRANF QSLDVL MAK +AP GTLTKTSIMLGCGETPDQ+V+TMEKVR
Sbjct: 242 ETVEELQSAVRDHRANFNQSLDVLRMAKDYAPAGTLTKTSIMLGCGETPDQIVKTMEKVR 301

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 302 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGE 361

Query: 361 FYIKSMIESDRAASN 376
           FYIKSMI+SDRA S+
Sbjct: 362 FYIKSMIDSDRAVSS 376

BLAST of Cla97C02G027660 vs. Swiss-Prot
Match: sp|A5CB81|LIAS_VITVI (Lipoyl synthase, mitochondrial OS=Vitis vinifera OX=29760 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 1.7e-170
Identity = 295/339 (87.02%), Postives = 312/339 (92.04%), Query Frame = 0

Query: 36  PRTLAGLRERLAAESPSLSDFVDLQSSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQI 95
           P+TLA LR RLA ESPSLSDFV LQ+SD YSVEVGTKKKPL KPKWMKES+PGG KY QI
Sbjct: 43  PQTLASLRHRLAVESPSLSDFVRLQTSDDYSVEVGTKKKPLSKPKWMKESIPGGAKYTQI 102

Query: 96  KKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRTPP 155
           KKKLR+L LHTVCEEA+CPN+GECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRT  
Sbjct: 103 KKKLRQLNLHTVCEEARCPNMGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRTXX 162

Query: 156 PPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVKKLKVLKPNMLIEALV 215
                  + VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETV+KLK+LKPNMLIEALV
Sbjct: 163 XXXXXXXSKVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKILKPNMLIEALV 222

Query: 216 PDFRGDPGCVEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKGFAPPGT 275
           PDFRGDPGCVEKV+KSGLDVFAHNIETVEELQ AVRDHRANFKQSL+VL +AK +A  GT
Sbjct: 223 PDFRGDPGCVEKVSKSGLDVFAHNIETVEELQSAVRDHRANFKQSLEVLKLAKEYADAGT 282

Query: 276 LTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQ 335
           LTKTSIMLGCGETPDQVV+TMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKY+
Sbjct: 283 LTKTSIMLGCGETPDQVVRTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYR 342

Query: 336 NLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAAS 375
            LGM MGFRYVASGPMVRSSYKAGEFYIKSMI++DRA S
Sbjct: 343 ILGMDMGFRYVASGPMVRSSYKAGEFYIKSMIDADRAMS 381

BLAST of Cla97C02G027660 vs. TAIR10
Match: AT2G20860.1 (lipoic acid synthase 1)

HSP 1 Score: 580.1 bits (1494), Expect = 1.0e-165
Identity = 285/341 (83.58%), Postives = 308/341 (90.32%), Query Frame = 0

Query: 34  QFPRTLAGLRERLAAESPSLSDFVDLQSSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYV 93
           Q P++L  LR RLA ESPSL+DF+     D+YSVEVGTKKKPLPKPKWMKES+PGGE+YV
Sbjct: 32  QSPKSLEALRARLANESPSLTDFI---HGDTYSVEVGTKKKPLPKPKWMKESIPGGERYV 91

Query: 94  QIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRT 153
           QIKKKLR+LKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTS  
Sbjct: 92  QIKKKLRDLKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSXX 151

Query: 154 PPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVKKLKVLKPNMLIEA 213
                     NVAEAIASWG+DYVVITSVDRDDLPDQGSGHFAETV++LK LKP MLIEA
Sbjct: 152 XXXXXXXXXXNVAEAIASWGVDYVVITSVDRDDLPDQGSGHFAETVQRLKFLKPEMLIEA 211

Query: 214 LVPDFRGDPGCVEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKGFAPP 273
           LVPDFRGD GCVEKV+KSGLDV AHNIETVEELQ  VRDHRANFKQSLDVL MAK +AP 
Sbjct: 212 LVPDFRGDGGCVEKVSKSGLDVLAHNIETVEELQSFVRDHRANFKQSLDVLRMAKEYAPA 271

Query: 274 GTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEK 333
           GTLTKTS+MLGCGETPDQVV+TMEKVRAAGVDVMTFGQYMRPSKRHMPV+EY+TP+AFE+
Sbjct: 272 GTLTKTSVMLGCGETPDQVVKTMEKVRAAGVDVMTFGQYMRPSKRHMPVAEYVTPDAFER 331

Query: 334 YQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAAS 375
           Y+ LGM+MGFRYVASGPMVRSSYKAGE+YIKSMIE+DR AS
Sbjct: 332 YRLLGMEMGFRYVASGPMVRSSYKAGEYYIKSMIEADRVAS 369

BLAST of Cla97C02G027660 vs. TAIR10
Match: AT5G08415.1 (Radical SAM superfamily protein)

HSP 1 Score: 384.0 bits (985), Expect = 1.1e-106
Identity = 176/297 (59.26%), Postives = 231/297 (77.78%), Query Frame = 0

Query: 78  KPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGD 137
           KP W+++  P GE++ ++K+ L  L L+TVCEEA+CPN+GECW+GG  G ATATIM+LGD
Sbjct: 97  KPAWLRQKAPQGERFQEVKESLSRLNLNTVCEEAQCPNIGECWNGGGDGVATATIMVLGD 156

Query: 138 TCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAE 197
           TCTRGCRFC VKTSR PPPPDP EP N A+AIASWG+DY+VITSVDRDD+PD GSGHFA+
Sbjct: 157 TCTRGCRFCAVKTSRNPPPPDPMEPENTAKAIASWGVDYIVITSVDRDDIPDGGSGHFAQ 216

Query: 198 TVKKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNIETVEELQGAVRDHRANF 257
           TVK +K  KP+++IE L  DFRGD   V+ +  SGLDVFAHN+ETV+ LQ  VRD RA +
Sbjct: 217 TVKAMKRHKPDIMIECLTSDFRGDLEAVDTLVHSGLDVFAHNVETVKRLQRLVRDPRAGY 276

Query: 258 KQSLDVLVMAKGFAPPGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSK 317
           +QS+ VL  AK  + PG +TKTSIMLG GET +++ + M  +RA  VD++T GQY++P+ 
Sbjct: 277 EQSMSVLKHAK-ISKPGMITKTSIMLGLGETDEELKEAMADLRAIDVDILTLGQYLQPTP 336

Query: 318 RHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAAS 375
            H+ V EY+TPE F+ ++  G  +GFRYVASGP+VRSSY+AGE ++K+M++   + S
Sbjct: 337 LHLTVKEYVTPEKFDFWKTYGESIGFRYVASGPLVRSSYRAGELFVKTMVKESYSKS 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_016901725.11.2e-20198.16PREDICTED: lipoyl synthase 2, mitochondrial isoform X1 [Cucumis melo][more]
XP_004136815.19.6e-19996.84PREDICTED: lipoyl synthase 2, mitochondrial [Cucumis sativus] >KGN43590.1 hypoth... [more]
XP_022988813.11.1e-19492.91lipoyl synthase 2, mitochondrial [Cucurbita maxima][more]
XP_023531015.12.5e-19492.89lipoyl synthase 2, mitochondrial [Cucurbita pepo subsp. pepo][more]
XP_022147922.14.2e-19494.21lipoyl synthase 2, mitochondrial [Momordica charantia][more]
Match NameE-valueIdentityDescription
tr|A0A1S4E162|A0A1S4E162_CUCME8.0e-20298.16Lipoyl synthase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495529 PE=3 SV=1[more]
tr|A0A0A0K1R3|A0A0A0K1R3_CUCSA6.4e-19996.84Lipoyl synthase, mitochondrial OS=Cucumis sativus OX=3659 GN=LIP1 PE=3 SV=1[more]
tr|A0A1R3J2J1|A0A1R3J2J1_COCAP2.6e-18486.13Lipoyl synthase, mitochondrial OS=Corchorus capsularis OX=210143 GN=LIP1 PE=3 SV... [more]
tr|A0A1R3JV88|A0A1R3JV88_9ROSI1.7e-18385.60Lipoyl synthase, mitochondrial OS=Corchorus olitorius OX=93759 GN=LIP1 PE=3 SV=1[more]
tr|A0A1U8BEC4|A0A1U8BEC4_NELNU1.6e-18190.12Lipoyl synthase, mitochondrial OS=Nelumbo nucifera OX=4432 GN=LOC104612573 PE=3 ... [more]
Match NameE-valueIdentityDescription
sp|B9H5L9|LIAS_POPTR3.9e-18390.14Lipoyl synthase, mitochondrial OS=Populus trichocarpa OX=3694 GN=LIP1 PE=3 SV=1[more]
sp|Q3LSN5|LIAS2_PEA3.5e-17688.30Lipoyl synthase 2, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-2 PE=2 SV=1[more]
sp|Q3LSN4|LIAS1_PEA4.6e-17688.30Lipoyl synthase 1, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-1 PE=2 SV=1[more]
sp|B7FM45|LIAS_MEDTR8.6e-17582.93Lipoyl synthase, mitochondrial OS=Medicago truncatula OX=3880 GN=LIP1 PE=2 SV=1[more]
sp|A5CB81|LIAS_VITVI1.7e-17087.02Lipoyl synthase, mitochondrial OS=Vitis vinifera OX=29760 GN=LIP1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G20860.11.0e-16583.58lipoic acid synthase 1[more]
AT5G08415.11.1e-10659.26Radical SAM superfamily protein[more]
The following terms have been associated with this gene:
Vocabulary: Cellular Component
TermDefinition
GO:0005739mitochondrion
Vocabulary: Molecular Function
TermDefinition
GO:00515394 iron, 4 sulfur cluster binding
GO:0016992lipoate synthase activity
GO:0003824catalytic activity
GO:0051536iron-sulfur cluster binding
Vocabulary: Biological Process
TermDefinition
GO:0009107lipoate biosynthetic process
Vocabulary: INTERPRO
TermDefinition
IPR027527Lipoyl_synth_mt
IPR013785Aldolase_TIM
IPR007197rSAM
IPR031691LIAS_N
IPR003698Lipoyl_synth
IPR006638Elp3/MiaB/NifB
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009107 lipoate biosynthetic process
biological_process GO:0019344 cysteine biosynthetic process
biological_process GO:0006766 vitamin metabolic process
biological_process GO:0006636 unsaturated fatty acid biosynthetic process
biological_process GO:0019748 secondary metabolic process
biological_process GO:0045893 positive regulation of transcription, DNA-templated
biological_process GO:0006733 oxidoreduction coenzyme metabolic process
biological_process GO:0009117 nucleotide metabolic process
biological_process GO:0009965 leaf morphogenesis
biological_process GO:0009695 jasmonic acid biosynthetic process
biological_process GO:0019288 isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
biological_process GO:0006546 glycine catabolic process
biological_process GO:0015995 chlorophyll biosynthetic process
biological_process GO:0009249 protein lipoylation
biological_process GO:0030154 cell differentiation
biological_process GO:0009073 aromatic amino acid family biosynthetic process
cellular_component GO:0005759 mitochondrial matrix
cellular_component GO:0005739 mitochondrion
cellular_component GO:0009507 chloroplast
cellular_component GO:0005575 cellular_component
molecular_function GO:0016740 transferase activity
molecular_function GO:0016783 sulfurtransferase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016992 lipoate synthase activity
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C02G027660.1Cla97C02G027660.1mRNA


Analysis Name: InterPro Annotations of watermelon 97103 v2
Date Performed: 2019-05-12
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006638Elp3/MiaB/NifBSMARTSM00729MiaBcoord: 129..336
e-value: 9.2E-10
score: 48.4
IPR003698Lipoyl synthasePIRSFPIRSF005963Lipoyl_synthcoord: 15..380
e-value: 6.0E-146
score: 483.3
IPR003698Lipoyl synthaseTIGRFAMTIGR00510TIGR00510coord: 72..366
e-value: 6.6E-121
score: 401.2
IPR003698Lipoyl synthaseSFLDSFLDF00271lipoyl_synthasecoord: 70..366
e-value: 0.0
score: 433.7
IPR003698Lipoyl synthaseHAMAPMF_00206Lipoyl_synthcoord: 78..372
score: 132.738
IPR031691Lipoyl synthase, N-terminalPFAMPF16881LIAS_Ncoord: 5..113
e-value: 1.5E-17
score: 63.8
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 136..298
e-value: 1.5E-11
score: 44.9
IPR007197Radical SAMSFLDSFLDS00029Radical_SAMcoord: 136..298
e-value: 8.7E-14
score: 46.8
IPR013785Aldolase-type TIM barrelGENE3DG3DSA:3.20.20.70coord: 122..355
e-value: 1.2E-14
score: 55.9
NoneNo IPR availableSFLDSFLDG01058lipoyl_synthase_likecoord: 70..366
e-value: 0.0
score: 433.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..36
NoneNo IPR availablePANTHERPTHR10949LIPOYL SYNTHASEcoord: 13..375
NoneNo IPR availablePANTHERPTHR10949:SF25LIPOYL SYNTHASE, MITOCHONDRIALcoord: 13..375
NoneNo IPR availableCDDcd01335Radical_SAMcoord: 133..313
e-value: 2.9274E-7
score: 48.4838
NoneNo IPR availableSUPERFAMILYSSF102114Radical SAM enzymescoord: 94..369
IPR027527Lipoyl synthase, mitochondrialHAMAPMF_03128Lipoyl_synth_plantMcoord: 20..375
score: 62.103

The following gene(s) are paralogous to this gene:

None