Cla97C01G004720 (gene) Watermelon (97103) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla97C01G004720
Type	gene
Organism	Citrullus lanatus (Watermelon (97103) v2)
Description	Prolyl 4-hydroxylase subunit alpha-1
Location	Cla97Chr01 : 4524020 .. 4526580 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTCTACAATAGCACCTTTCTGAAATCTATATCCACATTCTCTAATTTTTACTTCAACTTCATCTTGTTTCGATCTTCGTACCAGCCATCAATGGATTCTCGTCTTCATTTTTTGCTTCTTTTATCGATTGCATTTTCATTCTCAACCTGCCTTGCACAAAGGTGATGACCGAACCCCATTTTACATCGTATTTCGAAATCTTAGGTTTTACGTACTTATCTGTGGTTTACTGCCTCGATTGGATTCTCATCAATTTCGTCTACGGATATTTATTTGGAATTGTGTTCTTAAAACTGATTTTCGTTTCACTTTGGAATGATTTGTTGATGCATGATTGAGTTTCATAACATGGGTTTTTATTCTTTCCCTTGAATCTGTTATGTTCATTCTGCCAGCAATTTGATTAGTGGCCGGAAGGGTTTAAGGGACCAATTGGTTGAAAGACCTTTGAGCTACTCAAATCATTCAGGAAGAATCGACCCATCAAGAGTTGTCCAAGTCTCTTGGCGACCAAGGTCAGGTACTTGCAGAATCTCTCATATGATTTGGAATTATCACATCAACTTCAAAGCATATTGATTAACTTAGTAGGAAGTCTGATTTAACTTCTCTTGCTGTAGGGTTTTCTTGTATAAAGGTTTTCTCTCAGATGAGGAGTGTGATCATCTGATTTCTTTGGTATTTCTCTGCCCTGTTTTTGTTAGTATCATAAACATGCTTCCGCTTTTTCTGACGTGCATATACCTTTTGGGCTTAGGCTTCAAATTCAGAAGACAATCCTTCCGGGAACACTGTCTCAACCAAAGTGCTAAAGAGTTCAGGAGTCATTTTAAACACAACAGTATGTTCCTGTGCATTTTGAAGTTTATGCAATGGTTTGAATAGCACACTTAATGGTCAAATTTGTATTTCATAGCACGAGGTTTGTTTGTCTTGTTTTCTTGAAGCTTATGTTAAAGTAGTATATTTTTCAATCATATATTGTTTTCTGTTAGTTTCTACACGTGAGCAATTATGCAGTCCATCAGTTATAGAACTTTTTCTGTCATCATTGTTTTCTTTATTTCCAGTATTGTTATTCAAAATATAATTATAAATGTACATGGACGAACAGCTTCATCTATGCTTGGGAATTGCTTGAGTTAAATTAGTCGTTCAATGTATTTTCCATTGAAATATGTTCAGTCTAAAGTTCTCTGTTGATAGTTTTCTCAGTGGTACTTCCAAATACCGTGTTGCATATTAGGATTTGGTACACATACTGTTTTTGCTTTTAATTTCATATGCTGTCAATCTGTCTCAATACCCATAGAAGTATGCTTTTTTGCTGTATTTTCTTGGGGCTTGGTAGTCGTTTTAAAGGAATCTTAAATAACTCTGAACTCTAAATGTCCTGTGAAGTCAAACGTTCATTTCTGCATTTGTAGATAAGAACACTAAAAATGTGGAGGATGCTAGCATAGAAAAACCTATGATAACTTATGCAAAATTCCATAATTATTATTAAAATAATTATTTTTGATGATTCAATTTTGAGTTGTCAGCATCGTCTTTCAAAATTTATAGCAACCCATGGCTCAAGGGAGTAATTGCAGACCAAAGAATTTAGTATTGTGCTTCCTTTTTGTAAGACCATCTATTCCTGTTCATAGTATTTATGTTTTGTGGAGCTTGTCAATTACTTCTTAAGTAACTAGGTCTTGTGAGATAAAGCTCAAAGTCACTGTAGTTGGTCTATTTCTAGTTTCTATGAGATTTATTCTTTCTTGGTTGGGCTTGTTTCTTAGGATGATATTATTGCAAGAATTGAAAATCGAATTGCACTGTGGACTTTTCTCCCAAAAGGTATTTCTCATCAATGCTGTATTGCAGCTTTGCCTTTTTCATTTTCCTGATAATGCTCCTTTTATTTGGTTTTCTTTTCTTCTAAAAGTATTAGTTTTATTGTCTTCAGATCATAGCATGCCTTTCCAGATCATGCAATACAGGGGTGAAGAAGCAGAGCACAAGTACTTTTATGGCAACAGATCTGCAATGTCGTCCAGTGAGCCTTTGATGGCCACAGTAGTTTTGTATCTCTCAGATTCTGCTCGCGGTGGCGTGATGCTCTTTCCAGAGTCAAAGGTGAGGGGAAGTACTCAAAGATCTGTGGCCATAACAATGTACTCATGACTGCCTTTTTCTCAATGCCTCAGGTAAAGAGCAAATTTTGGTCAAACCGGAGAAAGAAAAACAACTTTCTGAGACCAGTGAAAGGCAATGCAGTACTTTTTTTCTCTGTGCATCTTAATGCTTCTCCAGACAAGAGTAACTACCACACTCGATCCCCAATACTCAATGGGGAATTGTGGGTTGCTACAAAATTCTTCTACTTAAGACCAACCACTGGGAATAAAGATAAACACACAATTGAATCTGATGTAGACGGTTGCATTGATGAAGATAAAAGCTGCCCCCAATGGGCTGCCATTGGCGAATGTGAACGAAACGCTGTATTCATGATCGGTTCTCCAGATTACTATGGTACATGTAGAAAAAGCTGCAATGCATGTTGA

mRNA sequence

ATGGATTTCTACAATAGCACCTTTCTGAAATCTATATCCACATTCTCTAATTTTTACTTCAACTTCATCTTGTTTCGATCTTCGTACCAGCCATCAATGGATTCTCGTCTTCATTTTTTGCTTCTTTTATCGATTGCATTTTCATTCTCAACCTGCCTTGCACAAAGCAATTTGATTAGTGGCCGGAAGGGTTTAAGGGACCAATTGGTTGAAAGACCTTTGAGCTACTCAAATCATTCAGGAAGAATCGACCCATCAAGAGTTGTCCAAGTCTCTTGGCGACCAAGGGTTTTCTTGTATAAAGGTTTTCTCTCAGATGAGGAGTGTGATCATCTGATTTCTTTGGCTTCAAATTCAGAAGACAATCCTTCCGGGAACACTGTCTCAACCAAAGTGCTAAAGAGTTCAGGAGTCATTTTAAACACAACAGATGATATTATTGCAAGAATTGAAAATCGAATTGCACTGTGGACTTTTCTCCCAAAAGATCATAGCATGCCTTTCCAGATCATGCAATACAGGGGTGAAGAAGCAGAGCACAAGTACTTTTATGGCAACAGATCTGCAATGTCGTCCAGTGAGCCTTTGATGGCCACAGTAGTTTTGTATCTCTCAGATTCTGCTCGCGGTGGCGTGATGCTCTTTCCAGAGTCAAAGGTAAAGAGCAAATTTTGGTCAAACCGGAGAAAGAAAAACAACTTTCTGAGACCAGTGAAAGGCAATGCAGTACTTTTTTTCTCTGTGCATCTTAATGCTTCTCCAGACAAGAGTAACTACCACACTCGATCCCCAATACTCAATGGGGAATTGTGGGTTGCTACAAAATTCTTCTACTTAAGACCAACCACTGGGAATAAAGATAAACACACAATTGAATCTGATGTAGACGGTTGCATTGATGAAGATAAAAGCTGCCCCCAATGGGCTGCCATTGGCGAATGTGAACGAAACGCTGTATTCATGATCGGTTCTCCAGATTACTATGGTACATGTAGAAAAAGCTGCAATGCATGTTGA

Coding sequence (CDS)

ATGGATTTCTACAATAGCACCTTTCTGAAATCTATATCCACATTCTCTAATTTTTACTTCAACTTCATCTTGTTTCGATCTTCGTACCAGCCATCAATGGATTCTCGTCTTCATTTTTTGCTTCTTTTATCGATTGCATTTTCATTCTCAACCTGCCTTGCACAAAGCAATTTGATTAGTGGCCGGAAGGGTTTAAGGGACCAATTGGTTGAAAGACCTTTGAGCTACTCAAATCATTCAGGAAGAATCGACCCATCAAGAGTTGTCCAAGTCTCTTGGCGACCAAGGGTTTTCTTGTATAAAGGTTTTCTCTCAGATGAGGAGTGTGATCATCTGATTTCTTTGGCTTCAAATTCAGAAGACAATCCTTCCGGGAACACTGTCTCAACCAAAGTGCTAAAGAGTTCAGGAGTCATTTTAAACACAACAGATGATATTATTGCAAGAATTGAAAATCGAATTGCACTGTGGACTTTTCTCCCAAAAGATCATAGCATGCCTTTCCAGATCATGCAATACAGGGGTGAAGAAGCAGAGCACAAGTACTTTTATGGCAACAGATCTGCAATGTCGTCCAGTGAGCCTTTGATGGCCACAGTAGTTTTGTATCTCTCAGATTCTGCTCGCGGTGGCGTGATGCTCTTTCCAGAGTCAAAGGTAAAGAGCAAATTTTGGTCAAACCGGAGAAAGAAAAACAACTTTCTGAGACCAGTGAAAGGCAATGCAGTACTTTTTTTCTCTGTGCATCTTAATGCTTCTCCAGACAAGAGTAACTACCACACTCGATCCCCAATACTCAATGGGGAATTGTGGGTTGCTACAAAATTCTTCTACTTAAGACCAACCACTGGGAATAAAGATAAACACACAATTGAATCTGATGTAGACGGTTGCATTGATGAAGATAAAAGCTGCCCCCAATGGGCTGCCATTGGCGAATGTGAACGAAACGCTGTATTCATGATCGGTTCTCCAGATTACTATGGTACATGTAGAAAAAGCTGCAATGCATGTTGA

Protein sequence

MDFYNSTFLKSISTFSNFYFNFILFRSSYQPSMDSRLHFLLLLSIAFSFSTCLAQSNLISGRKGLRDQLVERPLSYSNHSGRIDPSRVVQVSWRPRVFLYKGFLSDEECDHLISLASNSEDNPSGNTVSTKVLKSSGVILNTTDDIIARIENRIALWTFLPKDHSMPFQIMQYRGEEAEHKYFYGNRSAMSSSEPLMATVVLYLSDSARGGVMLFPESKVKSKFWSNRRKKNNFLRPVKGNAVLFFSVHLNASPDKSNYHTRSPILNGELWVATKFFYLRPTTGNKDKHTIESDVDGCIDEDKSCPQWAAIGECERNAVFMIGSPDYYGTCRKSCNAC

Homology

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BLAST of Cla97C01G004720 vs. NCBI nr
Match: XP_008436994.1 (PREDICTED: probable prolyl 4-hydroxylase 12 [Cucumis melo])

HSP 1 Score: 554.3 bits (1427), Expect = 2.9e-154
Identity = 274/313 (87.54%), Postives = 287/313 (91.69%), Query Frame = 0


Query: 33  MDSRLHFLLLLSIAFSFSTCLAQSNLISGRKGLRDQLVERPLSYSNHSGRIDPSRVVQVS 92
           MDSRL+FLLL + AFSFSTCLAQSNLISGRKGLRDQLV+RPLSYSN S RIDPSRVVQVS
Sbjct: 1   MDSRLNFLLLFATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSNQSVRIDPSRVVQVS 60

Query: 93  WRPRVFLYKGFLSDEECDHLISLASNSEDNP------SGNTVSTKVLKSSGVILNTTDDI 152
           WRPRVFLYKGFLSDEECDHLISLASNSEDNP      SGNTVST++L  SGVILNTTDDI
Sbjct: 61  WRPRVFLYKGFLSDEECDHLISLASNSEDNPSRNSAGSGNTVSTELLNGSGVILNTTDDI 120

Query: 153 IARIENRIALWTFLPKDHSMPFQIMQYRGEEAEHKYFYGNRSAM-SSSEPLMATVVLYLS 212
           IARIENRIA+WT LPKDH MPFQIMQYRGEEA+HKYFYGNRSAM SSSEPLMATVVLYLS
Sbjct: 121 IARIENRIAVWTLLPKDHGMPFQIMQYRGEEAKHKYFYGNRSAMSSSSEPLMATVVLYLS 180

Query: 213 DSARGGVMLFPESKVKSKFWSNRRKKNNFLRPVKGNAVLFFSVHLNASPDKSNYHTRSPI 272
           DSA GG MLFPESKVKSKFWS RRKK NFLRPVKGNA+LFFSVHLNASPDKS+YH R PI
Sbjct: 181 DSASGGEMLFPESKVKSKFWSGRRKKKNFLRPVKGNAILFFSVHLNASPDKSSYHIRYPI 240

Query: 273 LNGELWVATKFFYLRPTTGNKDKHTIESDVDGCIDEDKSCPQWAAIGECERNAVFMIGSP 332
            NGELWVATKF YLRP TGN  KHTI+S++DGCIDEDKSCPQWAAIGECERNAVFM+GSP
Sbjct: 241 RNGELWVATKFLYLRPPTGN--KHTIDSNIDGCIDEDKSCPQWAAIGECERNAVFMVGSP 300

Query: 333 DYYGTCRKSCNAC 339
           DYYGTCRKSCNAC
Sbjct: 301 DYYGTCRKSCNAC 311

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BLAST of Cla97C01G004720 vs. NCBI nr
Match: XP_004152378.1 (PREDICTED: probable prolyl 4-hydroxylase 12 [Cucumis sativus])

HSP 1 Score: 553.1 bits (1424), Expect = 6.4e-154
Identity = 272/313 (86.90%), Postives = 289/313 (92.33%), Query Frame = 0


Query: 33  MDSRLHFLLLLSIAFSFSTCLAQSNLISGRKGLRDQLVERPLSYSNHSGRIDPSRVVQVS 92
           MDSRL+FLLLL+ AFSFSTCLAQSNLISGRKGLRD+LV+RPLSYSN+SGRIDPSRVVQVS
Sbjct: 1   MDSRLNFLLLLATAFSFSTCLAQSNLISGRKGLRDRLVDRPLSYSNYSGRIDPSRVVQVS 60

Query: 93  WRPRVFLYKGFLSDEECDHLISLASNSEDNPSGN------TVSTKVLKSSGVILNTTDDI 152
           WRPRVFLYKGFLSDEECDHLISLASNSEDNPS N      TVST++L SSGVILNTTDDI
Sbjct: 61  WRPRVFLYKGFLSDEECDHLISLASNSEDNPSRNSAGSGITVSTELLNSSGVILNTTDDI 120

Query: 153 IARIENRIALWTFLPKDHSMPFQIMQYRGEEAEHKYFYGNRSAM-SSSEPLMATVVLYLS 212
           +ARIENR+A+WT LPKDHSMPFQIMQYRGEEA+HKYFYGNRSAM  SSEPLMATVVLYLS
Sbjct: 121 VARIENRLAIWTLLPKDHSMPFQIMQYRGEEAKHKYFYGNRSAMLPSSEPLMATVVLYLS 180

Query: 213 DSARGGVMLFPESKVKSKFWSNRRKKNNFLRPVKGNAVLFFSVHLNASPDKSNYHTRSPI 272
           DSA GG +LFPESKVKSKFWS RRKKNNFLRPVKGNA+LFFSVHLNASPDKS+YH RSPI
Sbjct: 181 DSASGGEILFPESKVKSKFWSGRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHIRSPI 240

Query: 273 LNGELWVATKFFYLRPTTGNKDKHTIESDVDGCIDEDKSCPQWAAIGECERNAVFMIGSP 332
            +GELWVATKF YL P  GN  KHTI+SDVDGC DEDKSCPQWAAIGECERNAVFM+GSP
Sbjct: 241 RDGELWVATKFLYLGPPAGN--KHTIQSDVDGCFDEDKSCPQWAAIGECERNAVFMVGSP 300

Query: 333 DYYGTCRKSCNAC 339
           DYYGTCRKSCNAC
Sbjct: 301 DYYGTCRKSCNAC 311

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BLAST of Cla97C01G004720 vs. NCBI nr
Match: XP_022159842.1 (probable prolyl 4-hydroxylase 12 [Momordica charantia])

HSP 1 Score: 523.5 bits (1347), Expect = 5.4e-145
Identity = 264/313 (84.35%), Postives = 279/313 (89.14%), Query Frame = 0


Query: 33  MDSRLHFLLLLSIAFSFSTCLAQSNLISGRKGLRDQLVER-PLSYSNHSGRIDPSRVVQV 92
           MDSRL  LLLL+ A SF +CLAQSNLISGRKGLRDQL+E  PLSYSNHSGRIDPSRVVQV
Sbjct: 1   MDSRLPVLLLLATAISFLSCLAQSNLISGRKGLRDQLIESVPLSYSNHSGRIDPSRVVQV 60

Query: 93  SWRPRVFLYKGFLSDEECDHLISLASNSEDNP------SGNTVSTKVLKSSGVILNTTDD 152
           SWRPRVFLYKGFLSDEECDHLISLA++SED P      SGNTV TK+LKSSG ILNTTDD
Sbjct: 61  SWRPRVFLYKGFLSDEECDHLISLATSSEDKPSGNSTDSGNTVPTKILKSSGAILNTTDD 120

Query: 153 IIARIENRIALWTFLPKDHSMPFQIMQYRGEEAEHKYFYGNRSAMSSSEPLMATVVLYLS 212
           IIARIENRIA+WTFLPKD+SMP QI+QY GEEAEHKY +GNRSAM SSEPLMATVVLYLS
Sbjct: 121 IIARIENRIAVWTFLPKDYSMPLQILQYGGEEAEHKYVFGNRSAMLSSEPLMATVVLYLS 180

Query: 213 DSARGGVMLFPESKVKSKFWSNRRKKNNFLRPVKGNAVLFFSVHLNASPDKSNYHTRSPI 272
           DSA GG M FPESKVKS+FWS+RRKKNN LRPVKGNAVL FSVHLNASPDKS+ HTRSPI
Sbjct: 181 DSASGGEMRFPESKVKSRFWSDRRKKNNILRPVKGNAVLIFSVHLNASPDKSSSHTRSPI 240

Query: 273 LNGELWVATKFFYLRPTTGNKDKHTIESDVDGCIDEDKSCPQWAAIGECERNAVFMIGSP 332
           L+GELW+ATKFFYLRP TGN  KHT E D D C DEDKSCPQWAAIGECERNAVFMIGSP
Sbjct: 241 LDGELWIATKFFYLRPITGN--KHTDEPDGD-CNDEDKSCPQWAAIGECERNAVFMIGSP 300

Query: 333 DYYGTCRKSCNAC 339
           DYYGTCRKSCNAC
Sbjct: 301 DYYGTCRKSCNAC 310

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BLAST of Cla97C01G004720 vs. NCBI nr
Match: KGN50302.1 (hypothetical protein Csa_5G166460 [Cucumis sativus])

HSP 1 Score: 518.1 bits (1333), Expect = 2.3e-143
Identity = 252/291 (86.60%), Postives = 268/291 (92.10%), Query Frame = 0


Query: 55  QSNLISGRKGLRDQLVERPLSYSNHSGRIDPSRVVQVSWRPRVFLYKGFLSDEECDHLIS 114
           +SNLISGRKGLRD+LV+RPLSYSN+SGRIDPSRVVQVSWRPRVFLYKGFLSDEECDHLIS
Sbjct: 6   KSNLISGRKGLRDRLVDRPLSYSNYSGRIDPSRVVQVSWRPRVFLYKGFLSDEECDHLIS 65

Query: 115 LASNSEDNPSGN------TVSTKVLKSSGVILNTTDDIIARIENRIALWTFLPKDHSMPF 174
           LASNSEDNPS N      TVST++L SSGVILNTTDDI+ARIENR+A+WT LPKDHSMPF
Sbjct: 66  LASNSEDNPSRNSAGSGITVSTELLNSSGVILNTTDDIVARIENRLAIWTLLPKDHSMPF 125

Query: 175 QIMQYRGEEAEHKYFYGNRSAM-SSSEPLMATVVLYLSDSARGGVMLFPESKVKSKFWSN 234
           QIMQYRGEEA+HKYFYGNRSAM  SSEPLMATVVLYLSDSA GG +LFPESKVKSKFWS 
Sbjct: 126 QIMQYRGEEAKHKYFYGNRSAMLPSSEPLMATVVLYLSDSASGGEILFPESKVKSKFWSG 185

Query: 235 RRKKNNFLRPVKGNAVLFFSVHLNASPDKSNYHTRSPILNGELWVATKFFYLRPTTGNKD 294
           RRKKNNFLRPVKGNA+LFFSVHLNASPDKS+YH RSPI +GELWVATKF YL P  GN  
Sbjct: 186 RRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHIRSPIRDGELWVATKFLYLGPPAGN-- 245

Query: 295 KHTIESDVDGCIDEDKSCPQWAAIGECERNAVFMIGSPDYYGTCRKSCNAC 339
           KHTI+SDVDGC DEDKSCPQWAAIGECERNAVFM+GSPDYYGTCRKSCNAC
Sbjct: 246 KHTIQSDVDGCFDEDKSCPQWAAIGECERNAVFMVGSPDYYGTCRKSCNAC 294

[Close]

BLAST of Cla97C01G004720 vs. NCBI nr
Match: XP_023549812.1 (probable prolyl 4-hydroxylase 12 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 503.8 bits (1296), Expect = 4.5e-139
Identity = 254/316 (80.38%), Postives = 279/316 (88.29%), Query Frame = 0


Query: 33  MDSRLHFLLLLSIAFSFSTCLAQSNLISGRKGLRDQLVER-PLSYSNHSGRIDPSRVVQV 92
           MDSRL+FLLL + AFSFS+CLAQSN +SGRKGLRDQ+V    LSYSNH  RIDPSRVVQ+
Sbjct: 1   MDSRLNFLLLFAAAFSFSSCLAQSNSVSGRKGLRDQMVNSGHLSYSNHFERIDPSRVVQI 60

Query: 93  SWRPRVFLYKGFLSDEECDHLISLASNSEDNP------SGNTVSTKVLKSSGVILNTTDD 152
           SW+PRVFLYKGFLSDEECDHLI+LASNSED P      S NTVSTK L +SG +LNTTDD
Sbjct: 61  SWQPRVFLYKGFLSDEECDHLIALASNSEDKPSRSNAGSRNTVSTKFLGNSGAVLNTTDD 120

Query: 153 IIARIENRIALWTFLPKDHSMPFQIMQYRGEEAE-HKYFYGNRSAMSSSEPLMATVVLYL 212
           IIARIENRIA+WTFLPKDHSMPFQIMQY GEEA  HKYF+GNRSAM SSEPLMATVVLYL
Sbjct: 121 IIARIENRIAVWTFLPKDHSMPFQIMQYGGEEAAGHKYFFGNRSAMPSSEPLMATVVLYL 180

Query: 213 SDSARGGVMLFPESKVKSKFWSNRRKKNNFLRPVKGNAVLFFSVHLNASPDKSNYHTRSP 272
           SDSA GG +LFP SKVK +FWS+RRKKNNFLRPVKGNAVLFFSVHLNASPDKS YH+R+P
Sbjct: 181 SDSASGGEILFPVSKVKRRFWSDRRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTP 240

Query: 273 ILNGELWVATKFFYLRP-TTGNKDKHTIESDV-DGCIDEDKSCPQWAAIGECERNAVFMI 332
           IL+G+LWVATKFFY+RP  TGN  +H +ES V D CIDED+SCP+WAAIGEC+RNAVFMI
Sbjct: 241 ILDGKLWVATKFFYIRPAATGN--EHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMI 300

Query: 333 GSPDYYGTCRKSCNAC 339
           GSPDYYGTCRKSCNAC
Sbjct: 301 GSPDYYGTCRKSCNAC 314

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BLAST of Cla97C01G004720 vs. TrEMBL
Match: tr|A0A1S3AT39|A0A1S3AT39_CUCME (probable prolyl 4-hydroxylase 12 OS=Cucumis melo OX=3656 GN=LOC103482556 PE=4 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 1.9e-154
Identity = 274/313 (87.54%), Postives = 287/313 (91.69%), Query Frame = 0


Query: 33  MDSRLHFLLLLSIAFSFSTCLAQSNLISGRKGLRDQLVERPLSYSNHSGRIDPSRVVQVS 92
           MDSRL+FLLL + AFSFSTCLAQSNLISGRKGLRDQLV+RPLSYSN S RIDPSRVVQVS
Sbjct: 1   MDSRLNFLLLFATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSNQSVRIDPSRVVQVS 60

Query: 93  WRPRVFLYKGFLSDEECDHLISLASNSEDNP------SGNTVSTKVLKSSGVILNTTDDI 152
           WRPRVFLYKGFLSDEECDHLISLASNSEDNP      SGNTVST++L  SGVILNTTDDI
Sbjct: 61  WRPRVFLYKGFLSDEECDHLISLASNSEDNPSRNSAGSGNTVSTELLNGSGVILNTTDDI 120

Query: 153 IARIENRIALWTFLPKDHSMPFQIMQYRGEEAEHKYFYGNRSAM-SSSEPLMATVVLYLS 212
           IARIENRIA+WT LPKDH MPFQIMQYRGEEA+HKYFYGNRSAM SSSEPLMATVVLYLS
Sbjct: 121 IARIENRIAVWTLLPKDHGMPFQIMQYRGEEAKHKYFYGNRSAMSSSSEPLMATVVLYLS 180

Query: 213 DSARGGVMLFPESKVKSKFWSNRRKKNNFLRPVKGNAVLFFSVHLNASPDKSNYHTRSPI 272
           DSA GG MLFPESKVKSKFWS RRKK NFLRPVKGNA+LFFSVHLNASPDKS+YH R PI
Sbjct: 181 DSASGGEMLFPESKVKSKFWSGRRKKKNFLRPVKGNAILFFSVHLNASPDKSSYHIRYPI 240

Query: 273 LNGELWVATKFFYLRPTTGNKDKHTIESDVDGCIDEDKSCPQWAAIGECERNAVFMIGSP 332
            NGELWVATKF YLRP TGN  KHTI+S++DGCIDEDKSCPQWAAIGECERNAVFM+GSP
Sbjct: 241 RNGELWVATKFLYLRPPTGN--KHTIDSNIDGCIDEDKSCPQWAAIGECERNAVFMVGSP 300

Query: 333 DYYGTCRKSCNAC 339
           DYYGTCRKSCNAC
Sbjct: 301 DYYGTCRKSCNAC 311

[Close]

BLAST of Cla97C01G004720 vs. TrEMBL
Match: tr|A0A0A0KPE4|A0A0A0KPE4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166460 PE=4 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.5e-143
Identity = 252/291 (86.60%), Postives = 268/291 (92.10%), Query Frame = 0


Query: 55  QSNLISGRKGLRDQLVERPLSYSNHSGRIDPSRVVQVSWRPRVFLYKGFLSDEECDHLIS 114
           +SNLISGRKGLRD+LV+RPLSYSN+SGRIDPSRVVQVSWRPRVFLYKGFLSDEECDHLIS
Sbjct: 6   KSNLISGRKGLRDRLVDRPLSYSNYSGRIDPSRVVQVSWRPRVFLYKGFLSDEECDHLIS 65

Query: 115 LASNSEDNPSGN------TVSTKVLKSSGVILNTTDDIIARIENRIALWTFLPKDHSMPF 174
           LASNSEDNPS N      TVST++L SSGVILNTTDDI+ARIENR+A+WT LPKDHSMPF
Sbjct: 66  LASNSEDNPSRNSAGSGITVSTELLNSSGVILNTTDDIVARIENRLAIWTLLPKDHSMPF 125

Query: 175 QIMQYRGEEAEHKYFYGNRSAM-SSSEPLMATVVLYLSDSARGGVMLFPESKVKSKFWSN 234
           QIMQYRGEEA+HKYFYGNRSAM  SSEPLMATVVLYLSDSA GG +LFPESKVKSKFWS 
Sbjct: 126 QIMQYRGEEAKHKYFYGNRSAMLPSSEPLMATVVLYLSDSASGGEILFPESKVKSKFWSG 185

Query: 235 RRKKNNFLRPVKGNAVLFFSVHLNASPDKSNYHTRSPILNGELWVATKFFYLRPTTGNKD 294
           RRKKNNFLRPVKGNA+LFFSVHLNASPDKS+YH RSPI +GELWVATKF YL P  GN  
Sbjct: 186 RRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHIRSPIRDGELWVATKFLYLGPPAGN-- 245

Query: 295 KHTIESDVDGCIDEDKSCPQWAAIGECERNAVFMIGSPDYYGTCRKSCNAC 339
           KHTI+SDVDGC DEDKSCPQWAAIGECERNAVFM+GSPDYYGTCRKSCNAC
Sbjct: 246 KHTIQSDVDGCFDEDKSCPQWAAIGECERNAVFMVGSPDYYGTCRKSCNAC 294

[Close]

BLAST of Cla97C01G004720 vs. TrEMBL
Match: tr|A0A2P4NAB4|A0A2P4NAB4_QUESU (Putative prolyl 4-hydroxylase 12 OS=Quercus suber OX=58331 GN=CFP56_60861 PE=4 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 6.0e-92
Identity = 176/317 (55.52%), Postives = 230/317 (72.56%), Query Frame = 0


Query: 33  MDSRLHFLLLLSIAFSFSTCLAQSNLISGRKGLRDQLVER----PLSYSNHSGRIDPSRV 92
           M S L  LLLL+   SF + LA+S     RK LRD+   +     L +S HS +IDPSRV
Sbjct: 1   MASLLSILLLLAFTSSFQSLLAES-----RKELRDKEASQETFIQLGHSVHSNKIDPSRV 60

Query: 93  VQVSWRPRVFLYKGFLSDEECDHLISLASNSEDN------PSGNTVSTKVLKSSGVILNT 152
           VQ+SW+PRVFLYKGFLSDEECDHLI+LA   ++N       SG+  + ++L+SS + L+ 
Sbjct: 61  VQLSWQPRVFLYKGFLSDEECDHLITLAHGMKENGLGNDDNSGHVGTDRLLRSSEIPLDI 120

Query: 153 TDDIIARIENRIALWTFLPKDHSMPFQIMQYRGEEAEHKYFY-GNRSAMSSSEPLMATVV 212
            DD+++RIE RI+ WTFLPK++S P QIM Y  EE + KY Y GN+S +  ++PLMA VV
Sbjct: 121 EDDVVSRIEERISAWTFLPKENSRPLQIMHYGLEEVDKKYNYLGNKSTLELTKPLMAIVV 180

Query: 213 LYLSDSARGGVMLFPESKVKSKFWSNRRKKNNFLRPVKGNAVLFFSVHLNASPDKSNYHT 272
           LYLS+  +GG + FP+S+VKSK WS   K +N LRP+KGNA+LFF+VH NASPD S+ H 
Sbjct: 181 LYLSNITQGGEIHFPDSEVKSKIWSGCTKSSNILRPIKGNAILFFTVHPNASPDNSSSHA 240

Query: 273 RSPILNGELWVATKFFYLRPTTGNKDKHTIESDVDGCIDEDKSCPQWAAIGECERNAVFM 332
           R PI+ GE+W ATKFF++   +G  +K  +++D   C DE++SCP+WAAIGEC+RN V+M
Sbjct: 241 RCPIVEGEMWHATKFFHVGSISG--EKLPLKTDGTDCTDEEESCPKWAAIGECQRNPVYM 300

Query: 333 IGSPDYYGTCRKSCNAC 339
           IGSPDYYGTCRKSCNAC
Sbjct: 301 IGSPDYYGTCRKSCNAC 310

[Close]

BLAST of Cla97C01G004720 vs. TrEMBL
Match: tr|A0A1R3HDG6|A0A1R3HDG6_COCAP (Metridin-like ShK toxin OS=Corchorus capsularis OX=210143 GN=CCACVL1_19977 PE=4 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.4e-88
Identity = 163/288 (56.60%), Postives = 205/288 (71.18%), Query Frame = 0


Query: 62  RKGLRDQLVER----PLSYSNHSGRIDPSRVVQVSWRPRVFLYKGFLSDEECDHLISLAS 121
           RK LRD++V+      L  S  S  IDPSRV Q+ WRPRVFLY GFLSDEECDHLISL  
Sbjct: 10  RKELRDKVVQEERVIQLRPSAESNTIDPSRVTQLLWRPRVFLYSGFLSDEECDHLISLGH 69

Query: 122 NSEDNPSG------NTVSTKVLKSSGVILNTTDDIIARIENRIALWTFLPKDHSMPFQIM 181
            +++   G      N  + + LKSS   LNT D ++A IE RI+ WTFLPKD+  P ++ 
Sbjct: 70  GAKEGMLGMNDVQANVGTNRQLKSSATSLNTEDKVLAMIEERISAWTFLPKDNGKPLEVR 129

Query: 182 QYRGEEAEHKY-FYGNRSAMSSSEPLMATVVLYLSDSARGGVMLFPESKVKSKFWSNRRK 241
            Y  EE +    ++GN+SA++ SEPLMATVVLYLS+  RGG +LFP S+ KSK WS   K
Sbjct: 130 HYGHEETKQNLDYFGNKSALALSEPLMATVVLYLSNVTRGGEILFPHSEPKSKMWSECTK 189

Query: 242 KNNFLRPVKGNAVLFFSVHLNASPDKSNYHTRSPILNGELWVATKFFYLRPTTGNKDKHT 301
            ++ L+P KGNA+LFF+ +LNASPD S+ H R P+L GE+W ATKFFY  P   N++K  
Sbjct: 190 TSSILKPAKGNAILFFTTNLNASPDGSSSHARCPVLEGEMWCATKFFY--PRAVNREKVP 249

Query: 302 IESDVDGCIDEDKSCPQWAAIGECERNAVFMIGSPDYYGTCRKSCNAC 339
            +SD +GC+DED +CPQWAA+GEC+RN VFM+GSPDYYGTCRKSCNAC
Sbjct: 250 FDSDGNGCVDEDTNCPQWAAVGECQRNPVFMVGSPDYYGTCRKSCNAC 295

[Close]

BLAST of Cla97C01G004720 vs. TrEMBL
Match: tr|A0A2I4HT83|A0A2I4HT83_9ROSI (probable prolyl 4-hydroxylase 12 isoform X2 OS=Juglans regia OX=51240 GN=LOC109021218 PE=4 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.2e-87
Identity = 168/309 (54.37%), Postives = 222/309 (71.84%), Query Frame = 0


Query: 40  LLLLSIAFSFSTCLAQSN--LISGRKGLRDQLVERPLSYSNHSGRIDPSRVVQVSWRPRV 99
           LLLL  A SF  C  +S+    SG++  ++ +++  L +S  S RIDPSRVVQ+SW+PRV
Sbjct: 8   LLLLVFASSFLICFTESSRKKFSGKQSNQETVIK--LGHSVDSNRIDPSRVVQLSWQPRV 67

Query: 100 FLYKGFLSDEECDHLISLASNSEDNPSGN------TVSTKVLKSSGVILNTTDDIIARIE 159
           FLYKGFLS EECDHLISL    +    GN       V+ ++L SS + LN  DD+++RIE
Sbjct: 68  FLYKGFLSVEECDHLISLVHGRKKEDLGNNGNSEHVVTNRLLMSSKMHLNIEDDVVSRIE 127

Query: 160 NRIALWTFLPKDHSMPFQIMQYRGEEAEHKY-FYGNRSAMSSSEPLMATVVLYLSDSARG 219
           +RI+ WTFLPK++S P Q+M Y  E+ +  Y F+GNR  +  +EPLMA +VLYLS+  +G
Sbjct: 128 DRISAWTFLPKENSRPLQVMHYGLEKVDRNYNFFGNRDLLGLTEPLMAIIVLYLSNVTQG 187

Query: 220 GVMLFPESKVKSKFWSNRRKKNNFLRPVKGNAVLFFSVHLNASPDKSNYHTRSPILNGEL 279
           G +LFPESK+K+  WS+    ++  RP+KGNA+LFF++H NASPDKS+ H R P+L GE+
Sbjct: 188 GEILFPESKLKNTIWSD-CTGSSIPRPIKGNAILFFTLHPNASPDKSSSHARCPVLEGEM 247

Query: 280 WVATKFFYLRPTTGNK-DKHTIESDVDGCIDEDKSCPQWAAIGECERNAVFMIGSPDYYG 339
           W ATKFF++R  +G K    +  SD  GCIDE ++CP+WAAIGEC+RN VFMIGSPDYYG
Sbjct: 248 WHATKFFHIRSISGEKVSPESDGSDDTGCIDEAENCPRWAAIGECQRNPVFMIGSPDYYG 307

[Close]

BLAST of Cla97C01G004720 vs. Swiss-Prot
Match: sp|Q8GXT7|P4H12_ARATH (Probable prolyl 4-hydroxylase 12 OS=Arabidopsis thaliana OX=3702 GN=P4H12 PE=2 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.3e-61
Identity = 132/310 (42.58%), Postives = 190/310 (61.29%), Query Frame = 0


Query: 35  SRLHFLLLLSIAFSFSTCLAQSNLISGRKGLRDQLV-----ERPLSYSNHSGRIDPSRVV 94
           SR+  +L+++++ S     +  +    RK LRD+ +     +   SY   S  +DP+RV+
Sbjct: 5   SRIFLILMITMSSSSPPFCSGGS----RKELRDKEITSKSDDTQASYVLGSKFVDPTRVL 64

Query: 95  QVSWRPRVFLYKGFLSDEECDHLISLASNSEDNPSGNTVSTKVLKSSGVILNTTDDIIAR 154
           Q+SW PRVFLY+GFLS+EECDHLISL             +T+V           D ++A 
Sbjct: 65  QLSWLPRVFLYRGFLSEEECDHLISLRKE----------TTEVYSVDADGKTQLDPVVAG 124

Query: 155 IENRIALWTFLPKDHSMPFQIMQYRGEEAEHKY-FYGNRSAMSSSEPLMATVVLYLSDSA 214
           IE +++ WTFLP ++    ++  Y  E++  K  ++G   +    E L+ATVVLYLS++ 
Sbjct: 125 IEEKVSAWTFLPGENGGSIKVRSYTSEKSGKKLDYFGEEPSSVLHESLLATVVLYLSNTT 184

Query: 215 RGGVMLFPESKVKSKFWSNRRKKNNFLRPVKGNAVLFFSVHLNASPDKSNYHTRSPILNG 274
           +GG +LFP S++K K  ++  +  N LRPVKGNA+LFF+  LNAS D  + H R P++ G
Sbjct: 185 QGGELLFPNSEMKPK--NSCLEGGNILRPVKGNAILFFTRLLNASLDGKSTHLRCPVVKG 244

Query: 275 ELWVATKFFYLRPTTGNKDKHTIESDVDGCIDEDKSCPQWAAIGECERNAVFMIGSPDYY 334
           EL VATK  Y       K +  IE   + C DED++C +WA +GEC++N V+MIGSPDYY
Sbjct: 245 ELLVATKLIYA------KKQARIEESGE-CSDEDENCGRWAKLGECKKNPVYMIGSPDYY 291

Query: 335 GTCRKSCNAC 339
           GTCRKSCNAC
Sbjct: 305 GTCRKSCNAC 291

[Close]

BLAST of Cla97C01G004720 vs. Swiss-Prot
Match: sp|Q8L970|P4H7_ARATH (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.6e-55
Identity = 123/323 (38.08%), Postives = 193/323 (59.75%), Query Frame = 0


Query: 33  MDSRLHFLLLLSIAFSFSTCL---AQSNLISGRKGLRDQLVERPLSYSNHSGRIDPSRVV 92
           MDSR+   L  S+ F F+  L   A +  ++     RD  V + +  S  S   DP+RV 
Sbjct: 1   MDSRI--FLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIK-MKTSASSFGFDPTRVT 60

Query: 93  QVSWRPRVFLYKGFLSDEECDHLISLA------SNSEDNPSGNTVSTKVLKSSGVILN-T 152
           Q+SW PRVFLY+GFLSDEECDH I LA      S   DN SG +V ++V  SSG+ L+  
Sbjct: 61  QLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKR 120

Query: 153 TDDIIARIENRIALWTFLPKDHSMPFQIMQY-RGEEAE-HKYFYGNRSAMSSSEPLMATV 212
            DDI++ +E ++A WTFLP+++    QI+ Y  G++ E H  ++ +++ +      +ATV
Sbjct: 121 QDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATV 180

Query: 213 VLYLSDSARGGVMLFP-----ESKVKSKFWSNRRKKNNFLRPVKGNAVLFFSVHLNASPD 272
           ++YLS+  +GG  +FP      +++K   W+   K+   ++P KG+A+LFF++H NA+ D
Sbjct: 181 LMYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHPNATTD 240

Query: 273 KSNYHTRSPILNGELWVATKFFYLRPTTGNKDKHTIESDVDGCIDEDKSCPQWAAIGECE 332
            ++ H   P++ GE W AT++ +++      +K +      GC+DE+ SC +WA  GEC+
Sbjct: 241 SNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQS------GCMDENVSCEKWAKAGECQ 300

Query: 333 RNAVFMIGSPDYYGTCRKSCNAC 339
           +N  +M+GS   +G CRKSC AC
Sbjct: 301 KNPTYMVGSDKDHGYCRKSCKAC 314

[Close]

BLAST of Cla97C01G004720 vs. Swiss-Prot
Match: sp|F4J0A8|P4H6_ARATH (Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 8.8e-54
Identity = 112/278 (40.29%), Postives = 165/278 (59.35%), Query Frame = 0


Query: 77  SNHSGRIDPSRVVQVSWRPRVFLYKGFLSDEECDHLISLASNS-------EDNPSGNTVS 136
           S+ S  +DP+R+ Q+SW PR FLYKGFLSDEECDHLI LA           D  SG +  
Sbjct: 21  SSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESED 80

Query: 137 TKVLKSSGVIL-NTTDDIIARIENRIALWTFLPKDHSMPFQIMQYRG---EEAEHKYFYG 196
           ++V  SSG+ L    DDI+A +E ++A WTFLP+++    QI+ Y      +    YFY 
Sbjct: 81  SEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFY- 140

Query: 197 NRSAMSSSEPLMATVVLYLSDSARGGVMLFPESK-----VKSKFWSNRRKKNNFLRPVKG 256
           ++ A+      +ATV++YLS+  +GG  +FP  K     +K   WS   K+   ++P KG
Sbjct: 141 DKKALELGGHRIATVLMYLSNVTKGGETVFPNWKGKTPQLKDDSWSKCAKQGYAVKPRKG 200

Query: 257 NAVLFFSVHLNASPDKSNYHTRSPILNGELWVATKFFYLRPTTGNKDKHTIESDVDGCID 316
           +A+LFF++HLN + D ++ H   P++ GE W AT++ ++R + G K           C+D
Sbjct: 201 DALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVR-SFGKKKL--------VCVD 260

Query: 317 EDKSCPQWAAIGECERNAVFMIGSPDYYGTCRKSCNAC 339
           + +SC +WA  GECE+N ++M+GS    G CRKSC AC
Sbjct: 261 DHESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

[Close]

BLAST of Cla97C01G004720 vs. Swiss-Prot
Match: sp|F4JAU3|P4H2_ARATH (Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.3e-46
Identity = 104/279 (37.28%), Postives = 171/279 (61.29%), Query Frame = 0


Query: 77  SNHSGRIDPSRVVQVSWRPRVFLYKGFLSDEECDHLISLASNS------EDNPSGNTVST 136
           S+ S  I+PS+V QVS +PR F+Y+GFL+D ECDHLISLA  +       DN +G +  +
Sbjct: 27  SSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVADNDNGESQVS 86

Query: 137 KVLKSSGVILNT-TDDIIARIENRIALWTFLPKDHSMPFQIMQY-RGEEAE-HKYFYGNR 196
            V  SSG  ++   D I++ IE++++ WTFLPK++    Q+++Y  G++ + H  ++ ++
Sbjct: 87  DVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYDAHFDYFHDK 146

Query: 197 SAMSSSEPLMATVVLYLSDSARGGVMLFPESKVKSK--------FWSNRRKKNNFLRPVK 256
             ++     +ATV+LYLS+  +GG  +FP+++  S+          S+  KK   ++P K
Sbjct: 147 VNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAKKGIAVKPKK 206

Query: 257 GNAVLFFSVHLNASPDKSNYHTRSPILNGELWVATKFFYLRPTTGNKDKHTIESDVDGCI 316
           GNA+LFF++  +A PD  + H   P++ GE W ATK+ ++     + DK  I +    C 
Sbjct: 207 GNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHV----DSFDK--ILTHDGNCT 266

Query: 317 DEDKSCPQWAAIGECERNAVFMIGSPDYYGTCRKSCNAC 339
           D ++SC +WA +GEC +N  +M+G+P+  G CR+SC AC
Sbjct: 267 DVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]

BLAST of Cla97C01G004720 vs. Swiss-Prot
Match: sp|Q8LAN3|P4H4_ARATH (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.5e-45
Identity = 99/280 (35.36%), Postives = 167/280 (59.64%), Query Frame = 0


Query: 77  SNHSGRIDPSRVVQVSWRPRVFLYKGFLSDEECDHLISLASNS------EDNPSGNTVST 136
           S+ S  ++PS+V QVS +PR F+Y+GFL++ ECDH++SLA  S       DN SG +  +
Sbjct: 26  SSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKFS 85

Query: 137 KVLKSSGVILNT-TDDIIARIENRIALWTFLPKDHSMPFQIMQY---RGEEAEHKYFYGN 196
           +V  SSG  ++   D I++ IE++I+ WTFLPK++    Q+++Y   +  +A   YF+  
Sbjct: 86  EVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDK 145

Query: 197 RSAMSSSEPLMATVVLYLSDSARGGVMLFPESKVKSK--------FWSNRRKKNNFLRPV 256
            + +      MAT+++YLS+  +GG  +FP++++ S+          S+  K+   ++P 
Sbjct: 146 VNIVRGGH-RMATILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKRGIAVKPR 205

Query: 257 KGNAVLFFSVHLNASPDKSNYHTRSPILNGELWVATKFFYLRPTTGNKDKHTIESDVDGC 316
           KG+A+LFF++H +A PD  + H   P++ GE W ATK+ ++           I +    C
Sbjct: 206 KGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHV------DSFDRIVTPSGNC 265

Query: 317 IDEDKSCPQWAAIGECERNAVFMIGSPDYYGTCRKSCNAC 339
            D ++SC +WA +GEC +N  +M+G+ +  G CR+SC AC
Sbjct: 266 TDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

[Close]

BLAST of Cla97C01G004720 vs. TAIR10
Match: AT4G25600.1 (Oxoglutarate/iron-dependent oxygenase)

HSP 1 Score: 236.5 bits (602), Expect = 2.4e-62
Identity = 132/310 (42.58%), Postives = 190/310 (61.29%), Query Frame = 0


Query: 35  SRLHFLLLLSIAFSFSTCLAQSNLISGRKGLRDQLV-----ERPLSYSNHSGRIDPSRVV 94
           SR+  +L+++++ S     +  +    RK LRD+ +     +   SY   S  +DP+RV+
Sbjct: 5   SRIFLILMITMSSSSPPFCSGGS----RKELRDKEITSKSDDTQASYVLGSKFVDPTRVL 64

Query: 95  QVSWRPRVFLYKGFLSDEECDHLISLASNSEDNPSGNTVSTKVLKSSGVILNTTDDIIAR 154
           Q+SW PRVFLY+GFLS+EECDHLISL             +T+V           D ++A 
Sbjct: 65  QLSWLPRVFLYRGFLSEEECDHLISLRKE----------TTEVYSVDADGKTQLDPVVAG 124

Query: 155 IENRIALWTFLPKDHSMPFQIMQYRGEEAEHKY-FYGNRSAMSSSEPLMATVVLYLSDSA 214
           IE +++ WTFLP ++    ++  Y  E++  K  ++G   +    E L+ATVVLYLS++ 
Sbjct: 125 IEEKVSAWTFLPGENGGSIKVRSYTSEKSGKKLDYFGEEPSSVLHESLLATVVLYLSNTT 184

Query: 215 RGGVMLFPESKVKSKFWSNRRKKNNFLRPVKGNAVLFFSVHLNASPDKSNYHTRSPILNG 274
           +GG +LFP S++K K  ++  +  N LRPVKGNA+LFF+  LNAS D  + H R P++ G
Sbjct: 185 QGGELLFPNSEMKPK--NSCLEGGNILRPVKGNAILFFTRLLNASLDGKSTHLRCPVVKG 244

Query: 275 ELWVATKFFYLRPTTGNKDKHTIESDVDGCIDEDKSCPQWAAIGECERNAVFMIGSPDYY 334
           EL VATK  Y       K +  IE   + C DED++C +WA +GEC++N V+MIGSPDYY
Sbjct: 245 ELLVATKLIYA------KKQARIEESGE-CSDEDENCGRWAKLGECKKNPVYMIGSPDYY 291

Query: 335 GTCRKSCNAC 339
           GTCRKSCNAC
Sbjct: 305 GTCRKSCNAC 291

[Close]

BLAST of Cla97C01G004720 vs. TAIR10
Match: AT3G28490.1 (Oxoglutarate/iron-dependent oxygenase)

HSP 1 Score: 212.2 bits (539), Expect = 4.9e-55
Identity = 112/278 (40.29%), Postives = 165/278 (59.35%), Query Frame = 0


Query: 77  SNHSGRIDPSRVVQVSWRPRVFLYKGFLSDEECDHLISLASNS-------EDNPSGNTVS 136
           S+ S  +DP+R+ Q+SW PR FLYKGFLSDEECDHLI LA           D  SG +  
Sbjct: 21  SSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESED 80

Query: 137 TKVLKSSGVIL-NTTDDIIARIENRIALWTFLPKDHSMPFQIMQYRG---EEAEHKYFYG 196
           ++V  SSG+ L    DDI+A +E ++A WTFLP+++    QI+ Y      +    YFY 
Sbjct: 81  SEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFY- 140

Query: 197 NRSAMSSSEPLMATVVLYLSDSARGGVMLFPESK-----VKSKFWSNRRKKNNFLRPVKG 256
           ++ A+      +ATV++YLS+  +GG  +FP  K     +K   WS   K+   ++P KG
Sbjct: 141 DKKALELGGHRIATVLMYLSNVTKGGETVFPNWKGKTPQLKDDSWSKCAKQGYAVKPRKG 200

Query: 257 NAVLFFSVHLNASPDKSNYHTRSPILNGELWVATKFFYLRPTTGNKDKHTIESDVDGCID 316
           +A+LFF++HLN + D ++ H   P++ GE W AT++ ++R + G K           C+D
Sbjct: 201 DALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVR-SFGKKKL--------VCVD 260

Query: 317 EDKSCPQWAAIGECERNAVFMIGSPDYYGTCRKSCNAC 339
           + +SC +WA  GECE+N ++M+GS    G CRKSC AC
Sbjct: 261 DHESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

[Close]

BLAST of Cla97C01G004720 vs. TAIR10
Match: AT3G28480.2 (Oxoglutarate/iron-dependent oxygenase)

HSP 1 Score: 209.9 bits (533), Expect = 2.4e-54
Identity = 123/331 (37.16%), Postives = 192/331 (58.01%), Query Frame = 0


Query: 33  MDSRLHFLLLLSIAFSFSTCL---AQSNLISGRKGLRDQLVERPLSYSNHSGRIDPSRVV 92
           MDSR+   L  S+ F F+  L   A +  ++     RD  V + +  S  S   DP+RV 
Sbjct: 1   MDSRI--FLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIK-MKTSASSFGFDPTRVT 60

Query: 93  QVSWRPRVFLYKGFLSDEECDHLISLA------SNSEDNPSGNTVSTK-----VLKSSGV 152
           Q+SW PRVFLY+GFLSDEECDH I LA      S   DN SG +V ++     V +SS  
Sbjct: 61  QLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEDSVSVVRQSSSF 120

Query: 153 ILN----TTDDIIARIENRIALWTFLPKDHSMPFQIMQY-RGEEAE-HKYFYGNRSAMSS 212
           I N      DDI++ +E ++A WTFLP+++    QI+ Y  G++ E H  ++ +++ +  
Sbjct: 121 IANMDSLEIDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLEL 180

Query: 213 SEPLMATVVLYLSDSARGGVMLFP-----ESKVKSKFWSNRRKKNNFLRPVKGNAVLFFS 272
               +ATV++YLS+  +GG  +FP      +++K   W+   K+   ++P KG+A+LFF+
Sbjct: 181 GGHRIATVLMYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFN 240

Query: 273 VHLNASPDKSNYHTRSPILNGELWVATKFFYLRPTTGNKDKHTIESDVDGCIDEDKSCPQ 332
           +H NA+ D ++ H   P++ GE W AT++ +++      +K +      GC+DE+ SC +
Sbjct: 241 LHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQS------GCMDENVSCEK 300

Query: 333 WAAIGECERNAVFMIGSPDYYGTCRKSCNAC 339
           WA  GEC++N  +M+GS   +G CRKSC AC
Sbjct: 301 WAKAGECQKNPTYMVGSDKDHGYCRKSCKAC 322

[Close]

BLAST of Cla97C01G004720 vs. TAIR10
Match: AT3G06300.1 (P4H isoform 2)

HSP 1 Score: 187.6 bits (475), Expect = 1.3e-47
Identity = 104/279 (37.28%), Postives = 171/279 (61.29%), Query Frame = 0


Query: 77  SNHSGRIDPSRVVQVSWRPRVFLYKGFLSDEECDHLISLASNS------EDNPSGNTVST 136
           S+ S  I+PS+V QVS +PR F+Y+GFL+D ECDHLISLA  +       DN +G +  +
Sbjct: 27  SSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVADNDNGESQVS 86

Query: 137 KVLKSSGVILNT-TDDIIARIENRIALWTFLPKDHSMPFQIMQY-RGEEAE-HKYFYGNR 196
            V  SSG  ++   D I++ IE++++ WTFLPK++    Q+++Y  G++ + H  ++ ++
Sbjct: 87  DVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYDAHFDYFHDK 146

Query: 197 SAMSSSEPLMATVVLYLSDSARGGVMLFPESKVKSK--------FWSNRRKKNNFLRPVK 256
             ++     +ATV+LYLS+  +GG  +FP+++  S+          S+  KK   ++P K
Sbjct: 147 VNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAKKGIAVKPKK 206

Query: 257 GNAVLFFSVHLNASPDKSNYHTRSPILNGELWVATKFFYLRPTTGNKDKHTIESDVDGCI 316
           GNA+LFF++  +A PD  + H   P++ GE W ATK+ ++     + DK  I +    C 
Sbjct: 207 GNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHV----DSFDK--ILTHDGNCT 266

Query: 317 DEDKSCPQWAAIGECERNAVFMIGSPDYYGTCRKSCNAC 339
           D ++SC +WA +GEC +N  +M+G+P+  G CR+SC AC
Sbjct: 267 DVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]

BLAST of Cla97C01G004720 vs. TAIR10
Match: AT5G18900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 184.9 bits (468), Expect = 8.3e-47
Identity = 99/280 (35.36%), Postives = 167/280 (59.64%), Query Frame = 0


Query: 77  SNHSGRIDPSRVVQVSWRPRVFLYKGFLSDEECDHLISLASNS------EDNPSGNTVST 136
           S+ S  ++PS+V QVS +PR F+Y+GFL++ ECDH++SLA  S       DN SG +  +
Sbjct: 26  SSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKFS 85

Query: 137 KVLKSSGVILNT-TDDIIARIENRIALWTFLPKDHSMPFQIMQY---RGEEAEHKYFYGN 196
           +V  SSG  ++   D I++ IE++I+ WTFLPK++    Q+++Y   +  +A   YF+  
Sbjct: 86  EVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDK 145

Query: 197 RSAMSSSEPLMATVVLYLSDSARGGVMLFPESKVKSK--------FWSNRRKKNNFLRPV 256
            + +      MAT+++YLS+  +GG  +FP++++ S+          S+  K+   ++P 
Sbjct: 146 VNIVRGGH-RMATILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKRGIAVKPR 205

Query: 257 KGNAVLFFSVHLNASPDKSNYHTRSPILNGELWVATKFFYLRPTTGNKDKHTIESDVDGC 316
           KG+A+LFF++H +A PD  + H   P++ GE W ATK+ ++           I +    C
Sbjct: 206 KGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHV------DSFDRIVTPSGNC 265

Query: 317 IDEDKSCPQWAAIGECERNAVFMIGSPDYYGTCRKSCNAC 339
            D ++SC +WA +GEC +N  +M+G+ +  G CR+SC AC
Sbjct: 266 TDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

The following BLAST results are available for this feature:

BLAST of Cla97C01G004720 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008436994.1	2.9e-154	87.54	PREDICTED: probable prolyl 4-hydroxylase 12 [Cucumis melo]	[more]
XP_004152378.1	6.4e-154	86.90	PREDICTED: probable prolyl 4-hydroxylase 12 [Cucumis sativus]	[more]
XP_022159842.1	5.4e-145	84.35	probable prolyl 4-hydroxylase 12 [Momordica charantia]	[more]
KGN50302.1	2.3e-143	86.60	hypothetical protein Csa_5G166460 [Cucumis sativus]	[more]
XP_023549812.1	4.5e-139	80.38	probable prolyl 4-hydroxylase 12 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cla97C01G004720 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3AT39\|A0A1S3AT39_CUCME	1.9e-154	87.54	probable prolyl 4-hydroxylase 12 OS=Cucumis melo OX=3656 GN=LOC103482556 PE=4 SV...	[more]
tr\|A0A0A0KPE4\|A0A0A0KPE4_CUCSA	1.5e-143	86.60	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166460 PE=4 SV=1	[more]
tr\|A0A2P4NAB4\|A0A2P4NAB4_QUESU	6.0e-92	55.52	Putative prolyl 4-hydroxylase 12 OS=Quercus suber OX=58331 GN=CFP56_60861 PE=4 S...	[more]
tr\|A0A1R3HDG6\|A0A1R3HDG6_COCAP	2.4e-88	56.60	Metridin-like ShK toxin OS=Corchorus capsularis OX=210143 GN=CCACVL1_19977 PE=4 ...	[more]
tr\|A0A2I4HT83\|A0A2I4HT83_9ROSI	1.2e-87	54.37	probable prolyl 4-hydroxylase 12 isoform X2 OS=Juglans regia OX=51240 GN=LOC1090...	[more]

BLAST of Cla97C01G004720 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q8GXT7\|P4H12_ARATH	4.3e-61	42.58	Probable prolyl 4-hydroxylase 12 OS=Arabidopsis thaliana OX=3702 GN=P4H12 PE=2 S...	[more]
sp\|Q8L970\|P4H7_ARATH	1.6e-55	38.08	Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=...	[more]
sp\|F4J0A8\|P4H6_ARATH	8.8e-54	40.29	Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=...	[more]
sp\|F4JAU3\|P4H2_ARATH	2.3e-46	37.28	Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1	[more]
sp\|Q8LAN3\|P4H4_ARATH	1.5e-45	35.36	Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=...	[more]

BLAST of Cla97C01G004720 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G25600.1	2.4e-62	42.58	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28490.1	4.9e-55	40.29	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28480.2	2.4e-54	37.16	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G06300.1	1.3e-47	37.28	P4H isoform 2	[more]
AT5G18900.1	8.3e-47	35.36	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506	iron ion binding
GO:0031418	L-ascorbic acid binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR003582	ShKT_dom
IPR006620	Pro_4_hyd_alph

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019511	peptidyl-proline hydroxylation
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0018401	peptidyl-proline hydroxylation to 4-hydroxy-L-proline
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005783	endoplasmic reticulum
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0004656	procollagen-proline 4-dioxygenase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C01G004720.1	Cla97C01G004720.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon 97103 v2
Date Performed: 2019-05-12


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	p4hc	coord: 95..278 e-value: 6.0E-13 score: 59.0
IPR003582	ShKT domain	SMART	SM00254	ShkT_1	coord: 297..338 e-value: 1.7E-4 score: 30.9
IPR003582	ShKT domain	PROSITE	PS51670	SHKT	coord: 298..338 score: 8.53
None	No IPR available	GENE3D	G3DSA:2.60.120.620		coord: 87..278 e-value: 1.3E-38 score: 134.9
None	No IPR available	PANTHER	PTHR10869:SF102	PROLYL 4-HYDROXYLASE 12-RELATED	coord: 40..338
None	No IPR available	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 40..338

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cla97C01G004720	Csa5G166460	Cucumber (Chinese Long) v2	cuwmbB336
Cla97C01G004720	MELO3C005478	Melon (DHL92) v3.5.1	mewmbB002
Cla97C01G004720	CSPI05G07280	Wild cucumber (PI 183967)	cpiwmbB349
Cla97C01G004720	Cucsa.129510	Cucumber (Gy14) v1	cgywmbB208
Cla97C01G004720	Lsi09G004730	Bottle gourd (USVL1VR-Ls)	lsiwmbB007
Cla97C01G004720	CsGy5G004710	Cucumber (Gy14) v2	cgybwmbB319
Cla97C01G004720	MELO3C005478.2	Melon (DHL92) v3.6.1	medwmbB000
Cla97C01G004720	Carg23177	Silver-seed gourd	carwmbB0569
Cla97C01G004720	CsaV3_5G004720	Cucumber (Chinese Long) v3	cucwmbB351
Cla97C01G004720	Bhi12G000444	Wax gourd	wgowmbB065

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla97C01G004720	Cucurbita maxima (Rimu)	cmawmbB288
Cla97C01G004720	Cucurbita moschata (Rifu)	cmowmbB270
Cla97C01G004720	Watermelon (Charleston Gray)	wcgwmbB089
Cla97C01G004720	Watermelon (97103) v1	wmwmbB245