Carg23177 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg23177
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Prolyl 4-hydroxylase alpha subunit, putative
Location	Cucurbita_argyrosperma_scaffold_144 : 164173 .. 166756 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GATTCATGGTAGTACAGAGGAGACCATGGATTTGTTCAGCAGCAGCTTCCTGAAAATCTGTATCCACAACTTCTAATTCTTCATACCTTTTCAACTTCCTCTTCCTCGTCTCTTCTTTTTGGATCTTCGTCTCACCCATCCATGGATTCTCGTCTCAACTTTTTGCTTCTTTTAGCGGCCGCATTTTCATTCTCGAGCTGCCTTGCACAAAGGTGATTTCCGAACGCAATTTACATCGAACTCCGGTTTTACAGATCCGCTTCTCCGCATCTTTTGTGTTGTGTACTGCCTGGATTGGATTCTCGTCAATTTTGTCTACGGAATTACAATCGGAATTGTGTTCTATTACTGATTTTTGTTTCACTGCATGATACTGAAATTGTTGAATGATTTGTTAATGCATGGCTGTGTTTGATATCGTGTGTTTTTCCTCTTTCTATTGAATCTGTTCTGTTCTTTCTGCCAGCAATTCGATTAGTGGCCGTAAGGGTTTAAGGGACCAAATGGTTAACAGTGGACATTTGAGCTACTCAAATCATTCTGAAAGAATCGACCCATCACGAGTTGTCCAAATCTCTTGGCAACCAAGGTCAGGTACTTGCGAATCTCTCATATGATTTAGAATTATCACATCAACTTCAGAGCATATTGATTAACTTAGAAGAAAATCTGATTTAACTTTTGTTGCTTTAGGGCCTTCTTGTATAAAGGCTTTCTCTCAGATGAGGAGTGTGATCACCTTATTGCTTTGGTACTTTTCTGCCCTGTTCTTGTTAGTATCATGGACATGATTAAGCTGTTTCTGACTTGCATTTAACTTTTGGGCTTAGGCTTCAAATTCTGAAGATAAACCTTCTAGGAACAATGCTGGTTCCAGGAACACTGTCTCAACCAAATTTCTAGGCAATTCAGGAGCTATTTTAAACACAACAGTACATTCCTGTGCCTTTTGAAGTTTATGCAATGGTTTAGTTGTACACCTAGAGGTCAAATTTATATTTCATAGCATGAAATTTGCTTCTCTTGTTTTCTTGAAGTTTTTGTTGAATTAGCATACTTTTCAATCGTGTATTGTTTTCTGTTAGTTTCTACATGTGAGCAATTATACAGAACATCAGTTTTAAAACTTCTTTGGTCATTACTGTTTTATTTATTTCCAGTGCTGTTATTCAAAATATAACCATAAACGTACGTGGATGAACATTTTTATCTGTGCTTGGCAATTGTTTGAGTTAAATTAGCCGTTCAATTTATTTTCCATTGAAATATGCTGATATGTTCGGTCTAAAGCTTTCTGTTGATATGTTCATACAACCTTTCTTCTCATTCCTTCAATTTTCTTGATGGATCACATTTAGGATTGATGACATTTCATATGCTATCAACTCGTTCTAATACTCAAAGAAGCATGCTTTGTTGCTATATTTTCCGGGGAACCGATAGGAGCTTGATAGGGATCTCAAACAACTCTAGCTGCAAATGTCCTATGGACACAAACATGATATAAACACTAAAAATGTGATGGTAGTTAGCATATCCTATGATAATTTACGCATGATTTCGAAATTATTGTTCTTGATGACTCCACTCTTAGTTGTTGGAATCAATTATCTTGCAAAATCTATAACAAACCATGGCTCAAGGGAGGAACTGTGGTTCCTTTTTGTAGTACCTAACTATTTCTGTTCACGGTATGTATGTATTGTGGAGCTTGTCAACTGCTTCTTATAGTAACTAAGCCTTGTCAGATAAAAGCTCTGAACCACGGTGGTCGGTCTATTTCATGTTTCTTGCAGATTTATACTTTGTTTTCTTATTTGGGTTTGTTTCTTAGGATGATATCATTGCCAGGATTGAAAATAGAATTGCGGTGTGGACTTTTCTCCCAAAAGGTATTTTTCTCGTCAGTGCTGTATTGAAACTGGCTTGGCCATCTGCATTTTCCTTATTATTCCCCTTTCATTTGATCTTCAGATCATAGCATGCCTTTCCAGATTATGCAATACGGGGGTGAAGAAGCAGCAGGGCATAAGTACTTTTTTGGCAACAGATCTGCAATGCCATCCAGTGAACCGTTGATGGCCACGGTAGTTTTGTATCTATCAGATTCGGCCAGCGGTGGCGAGATTCTGTTCCCAGTATCAAAGGTGAGACGAAATACCGAAAGATCCATGGCCATAACAATGTACTACTCATGACTGGCTTCTTTTCAATGCTTCAGGTAAAGAGAAGATTTTGGTCAGACCAGAGAAAGAAAAACAACTTTCTGAGACCAGTGAAAGGCAATGCAGTTCTTTTTTTCTCTGTTCATCTTAATGCTTCTCCAGACAAGAGTTGCTACCATTCCCGAACGCCAATACTCGATGGGAAATTGTGGGTTGCTACAAAATTCTTCTACATAAGACCAGCAGCCACTGGGAATGAACACGCAGTTGAATCCGGTGTAGACGACGACTGCATTGATGAAGATGAAAGCTGCCCCAAATGGGCTGCCATCGGCGAATGCAAACGAAACGCGGTGTTCATGATCGGTTCTCCAGATTACTATGGCACATGTAGAAAAAGCTGCAACGCATGTGGATGA

mRNA sequence

GATTCATGGTAGTACAGAGGAGACCATGGATTTGTTCAGCAGCAGCTTCCTGAAAATCTGTATCCACAACTTCTAATTCTTCATACCTTTTCAACTTCCTCTTCCTCGTCTCTTCTTTTTGGATCTTCGTCTCACCCATCCATGGATTCTCGTCTCAACTTTTTGCTTCTTTTAGCGGCCGCATTTTCATTCTCGAGCTGCCTTGCACAAAGCAATTCGATTAGTGGCCGTAAGGGTTTAAGGGACCAAATGGTTAACAGTGGACATTTGAGCTACTCAAATCATTCTGAAAGAATCGACCCATCACGAGTTGTCCAAATCTCTTGGCAACCAAGGGCCTTCTTGTATAAAGGCTTTCTCTCAGATGAGGAGTGTGATCACCTTATTGCTTTGGCTTCAAATTCTGAAGATAAACCTTCTAGGAACAATGCTGGTTCCAGGAACACTGTCTCAACCAAATTTCTAGGCAATTCAGGAGCTATTTTAAACACAACAGATGATATCATTGCCAGGATTGAAAATAGAATTGCGGTGTGGACTTTTCTCCCAAAAGATCATAGCATGCCTTTCCAGATTATGCAATACGGGGGTGAAGAAGCAGCAGGGCATAAGTACTTTTTTGGCAACAGATCTGCAATGCCATCCAGTGAACCGTTGATGGCCACGGTAGTTTTGTATCTATCAGATTCGGCCAGCGGTGGCGAGATTCTGTTCCCAGTATCAAAGGTAAAGAGAAGATTTTGGTCAGACCAGAGAAAGAAAAACAACTTTCTGAGACCAGTGAAAGGCAATGCAGTTCTTTTTTTCTCTGTTCATCTTAATGCTTCTCCAGACAAGAGTTGCTACCATTCCCGAACGCCAATACTCGATGGGAAATTGTGGGTTGCTACAAAATTCTTCTACATAAGACCAGCAGCCACTGGGAATGAACACGCAGTTGAATCCGGTGTAGACGACGACTGCATTGATGAAGATGAAAGCTGCCCCAAATGGGCTGCCATCGGCGAATGCAAACGAAACGCGGTGTTCATGATCGGTTCTCCAGATTACTATGGCACATGTAGAAAAAGCTGCAACGCATGTGGATGA

Coding sequence (CDS)

ATGGATTCTCGTCTCAACTTTTTGCTTCTTTTAGCGGCCGCATTTTCATTCTCGAGCTGCCTTGCACAAAGCAATTCGATTAGTGGCCGTAAGGGTTTAAGGGACCAAATGGTTAACAGTGGACATTTGAGCTACTCAAATCATTCTGAAAGAATCGACCCATCACGAGTTGTCCAAATCTCTTGGCAACCAAGGGCCTTCTTGTATAAAGGCTTTCTCTCAGATGAGGAGTGTGATCACCTTATTGCTTTGGCTTCAAATTCTGAAGATAAACCTTCTAGGAACAATGCTGGTTCCAGGAACACTGTCTCAACCAAATTTCTAGGCAATTCAGGAGCTATTTTAAACACAACAGATGATATCATTGCCAGGATTGAAAATAGAATTGCGGTGTGGACTTTTCTCCCAAAAGATCATAGCATGCCTTTCCAGATTATGCAATACGGGGGTGAAGAAGCAGCAGGGCATAAGTACTTTTTTGGCAACAGATCTGCAATGCCATCCAGTGAACCGTTGATGGCCACGGTAGTTTTGTATCTATCAGATTCGGCCAGCGGTGGCGAGATTCTGTTCCCAGTATCAAAGGTAAAGAGAAGATTTTGGTCAGACCAGAGAAAGAAAAACAACTTTCTGAGACCAGTGAAAGGCAATGCAGTTCTTTTTTTCTCTGTTCATCTTAATGCTTCTCCAGACAAGAGTTGCTACCATTCCCGAACGCCAATACTCGATGGGAAATTGTGGGTTGCTACAAAATTCTTCTACATAAGACCAGCAGCCACTGGGAATGAACACGCAGTTGAATCCGGTGTAGACGACGACTGCATTGATGAAGATGAAAGCTGCCCCAAATGGGCTGCCATCGGCGAATGCAAACGAAACGCGGTGTTCATGATCGGTTCTCCAGATTACTATGGCACATGTAGAAAAAGCTGCAACGCATGTGGATGA

Protein sequence

MDSRLNFLLLLAAAFSFSSCLAQSNSISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQISWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDDIIARIENRIAVWTFLPKDHSMPFQIMQYGGEEAAGHKYFFGNRSAMPSSEPLMATVVLYLSDSASGGEILFPVSKVKRRFWSDQRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTPILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGSPDYYGTCRKSCNACG

Homology

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BLAST of Carg23177 vs. NCBI nr
Match: XP_022922237.1 (probable prolyl 4-hydroxylase 12 [Cucurbita moschata])

HSP 1 Score: 642.1 bits (1655), Expect = 9.8e-181
Identity = 311/315 (98.73%), Postives = 313/315 (99.37%), Query Frame = 0


Query: 1   MDSRLNFLLLLAAAFSFSSCLAQSNSISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQI 60
           MDSRL FLLLLAAAFSFSSCLAQSNSISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQI
Sbjct: 1   MDSRLTFLLLLAAAFSFSSCLAQSNSISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQI 60

Query: 61  SWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDD 120
           SWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDD
Sbjct: 61  SWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDD 120

Query: 121 IIARIENRIAVWTFLPKDHSMPFQIMQYGGEEAAGHKYFFGNRSAMPSSEPLMATVVLYL 180
           II RIENRIAVWTFLPKDHSMPFQIM+YGGEEAAGHKYFFGNRSAMPSSEPLMATVVLYL
Sbjct: 121 IIGRIENRIAVWTFLPKDHSMPFQIMKYGGEEAAGHKYFFGNRSAMPSSEPLMATVVLYL 180

Query: 181 SDSASGGEILFPVSKVKRRFWSDQRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTP 240
           SDSASGGEILFPVSKVKRRFWSD+RKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTP
Sbjct: 181 SDSASGGEILFPVSKVKRRFWSDRRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTP 240

Query: 241 ILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGS 300
           ILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGS
Sbjct: 241 ILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGS 300

Query: 301 PDYYGTCRKSCNACG 316
           PDYYGTCRKSCNACG
Sbjct: 301 PDYYGTCRKSCNACG 315

[Close]

BLAST of Carg23177 vs. NCBI nr
Match: XP_023549812.1 (probable prolyl 4-hydroxylase 12 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 639.4 bits (1648), Expect = 6.3e-180
Identity = 308/315 (97.78%), Postives = 312/315 (99.05%), Query Frame = 0


Query: 1   MDSRLNFLLLLAAAFSFSSCLAQSNSISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQI 60
           MDSRLNFLLL AAAFSFSSCLAQSNS+SGRKGLRDQMVNSGHLSYSNH ERIDPSRVVQI
Sbjct: 1   MDSRLNFLLLFAAAFSFSSCLAQSNSVSGRKGLRDQMVNSGHLSYSNHFERIDPSRVVQI 60

Query: 61  SWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDD 120
           SWQPR FLYKGFLSDEECDHLIALASNSEDKPSR+NAGSRNTVSTKFLGNSGA+LNTTDD
Sbjct: 61  SWQPRVFLYKGFLSDEECDHLIALASNSEDKPSRSNAGSRNTVSTKFLGNSGAVLNTTDD 120

Query: 121 IIARIENRIAVWTFLPKDHSMPFQIMQYGGEEAAGHKYFFGNRSAMPSSEPLMATVVLYL 180
           IIARIENRIAVWTFLPKDHSMPFQIMQYGGEEAAGHKYFFGNRSAMPSSEPLMATVVLYL
Sbjct: 121 IIARIENRIAVWTFLPKDHSMPFQIMQYGGEEAAGHKYFFGNRSAMPSSEPLMATVVLYL 180

Query: 181 SDSASGGEILFPVSKVKRRFWSDQRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTP 240
           SDSASGGEILFPVSKVKRRFWSD+RKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTP
Sbjct: 181 SDSASGGEILFPVSKVKRRFWSDRRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTP 240

Query: 241 ILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGS 300
           ILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGS
Sbjct: 241 ILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGS 300

Query: 301 PDYYGTCRKSCNACG 316
           PDYYGTCRKSCNACG
Sbjct: 301 PDYYGTCRKSCNACG 315

[Close]

BLAST of Carg23177 vs. NCBI nr
Match: XP_022973003.1 (probable prolyl 4-hydroxylase 12 [Cucurbita maxima])

HSP 1 Score: 639.0 bits (1647), Expect = 8.3e-180
Identity = 310/315 (98.41%), Postives = 312/315 (99.05%), Query Frame = 0


Query: 1   MDSRLNFLLLLAAAFSFSSCLAQSNSISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQI 60
           MDSRLNFLLLLAAAFSF SCLAQSNSISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQI
Sbjct: 1   MDSRLNFLLLLAAAFSFPSCLAQSNSISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQI 60

Query: 61  SWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDD 120
           SWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDD
Sbjct: 61  SWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDD 120

Query: 121 IIARIENRIAVWTFLPKDHSMPFQIMQYGGEEAAGHKYFFGNRSAMPSSEPLMATVVLYL 180
           IIARIENRIAVW FLPKDHSMPFQIMQYGGEEAAG KYFFGNRSAMPSSEPLMATVVLYL
Sbjct: 121 IIARIENRIAVWLFLPKDHSMPFQIMQYGGEEAAGRKYFFGNRSAMPSSEPLMATVVLYL 180

Query: 181 SDSASGGEILFPVSKVKRRFWSDQRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTP 240
           SDSA+GGEILFPVSKVKRRFWSD+RKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTP
Sbjct: 181 SDSANGGEILFPVSKVKRRFWSDRRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTP 240

Query: 241 ILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGS 300
           ILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGS
Sbjct: 241 ILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGS 300

Query: 301 PDYYGTCRKSCNACG 316
           PDYYGTCRKSCNACG
Sbjct: 301 PDYYGTCRKSCNACG 315

[Close]

BLAST of Carg23177 vs. NCBI nr
Match: XP_004152378.1 (PREDICTED: probable prolyl 4-hydroxylase 12 [Cucumis sativus])

HSP 1 Score: 514.2 bits (1323), Expect = 3.1e-142
Identity = 254/315 (80.63%), Postives = 282/315 (89.52%), Query Frame = 0


Query: 1   MDSRLNFLLLLAAAFSFSSCLAQSNSISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQI 60
           MDSRLNFLLLLA AFSFS+CLAQSN ISGRKGLRD++V+   LSYSN+S RIDPSRVVQ+
Sbjct: 1   MDSRLNFLLLLATAFSFSTCLAQSNLISGRKGLRDRLVDR-PLSYSNYSGRIDPSRVVQV 60

Query: 61  SWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDD 120
           SW+PR FLYKGFLSDEECDHLI+LASNSED PSRN+AGS  TVST+ L +SG ILNTTDD
Sbjct: 61  SWRPRVFLYKGFLSDEECDHLISLASNSEDNPSRNSAGSGITVSTELLNSSGVILNTTDD 120

Query: 121 IIARIENRIAVWTFLPKDHSMPFQIMQYGGEEAAGHKYFFGNRSAM-PSSEPLMATVVLY 180
           I+ARIENR+A+WT LPKDHSMPFQIMQY GEEA  HKYF+GNRSAM PSSEPLMATVVLY
Sbjct: 121 IVARIENRLAIWTLLPKDHSMPFQIMQYRGEEAK-HKYFYGNRSAMLPSSEPLMATVVLY 180

Query: 181 LSDSASGGEILFPVSKVKRRFWSDQRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRT 240
           LSDSASGGEILFP SKVK +FWS +RKKNNFLRPVKGNA+LFFSVHLNASPDKS YH R+
Sbjct: 181 LSDSASGGEILFPESKVKSKFWSGRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHIRS 240

Query: 241 PILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIG 300
           PI DG+LWVATKF Y+ P A GN+H ++S V D C DED+SCP+WAAIGEC+RNAVFM+G
Sbjct: 241 PIRDGELWVATKFLYLGPPA-GNKHTIQSDV-DGCFDEDKSCPQWAAIGECERNAVFMVG 300

Query: 301 SPDYYGTCRKSCNAC 315
           SPDYYGTCRKSCNAC
Sbjct: 301 SPDYYGTCRKSCNAC 311

[Close]

BLAST of Carg23177 vs. NCBI nr
Match: XP_008436994.1 (PREDICTED: probable prolyl 4-hydroxylase 12 [Cucumis melo])

HSP 1 Score: 511.5 bits (1316), Expect = 2.0e-141
Identity = 254/315 (80.63%), Postives = 278/315 (88.25%), Query Frame = 0


Query: 1   MDSRLNFLLLLAAAFSFSSCLAQSNSISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQI 60
           MDSRLNFLLL A AFSFS+CLAQSN ISGRKGLRDQ+V+   LSYSN S RIDPSRVVQ+
Sbjct: 1   MDSRLNFLLLFATAFSFSTCLAQSNLISGRKGLRDQLVDR-PLSYSNQSVRIDPSRVVQV 60

Query: 61  SWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDD 120
           SW+PR FLYKGFLSDEECDHLI+LASNSED PSRN+AGS NTVST+ L  SG ILNTTDD
Sbjct: 61  SWRPRVFLYKGFLSDEECDHLISLASNSEDNPSRNSAGSGNTVSTELLNGSGVILNTTDD 120

Query: 121 IIARIENRIAVWTFLPKDHSMPFQIMQYGGEEAAGHKYFFGNRSAM-PSSEPLMATVVLY 180
           IIARIENRIAVWT LPKDH MPFQIMQY GEEA  HKYF+GNRSAM  SSEPLMATVVLY
Sbjct: 121 IIARIENRIAVWTLLPKDHGMPFQIMQYRGEEAK-HKYFYGNRSAMSSSSEPLMATVVLY 180

Query: 181 LSDSASGGEILFPVSKVKRRFWSDQRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRT 240
           LSDSASGGE+LFP SKVK +FWS +RKK NFLRPVKGNA+LFFSVHLNASPDKS YH R 
Sbjct: 181 LSDSASGGEMLFPESKVKSKFWSGRRKKKNFLRPVKGNAILFFSVHLNASPDKSSYHIRY 240

Query: 241 PILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIG 300
           PI +G+LWVATKF Y+RP  TGN+H ++S + D CIDED+SCP+WAAIGEC+RNAVFM+G
Sbjct: 241 PIRNGELWVATKFLYLRP-PTGNKHTIDSNI-DGCIDEDKSCPQWAAIGECERNAVFMVG 300

Query: 301 SPDYYGTCRKSCNAC 315
           SPDYYGTCRKSCNAC
Sbjct: 301 SPDYYGTCRKSCNAC 311

[Close]

BLAST of Carg23177 vs. TAIR10
Match: AT4G25600.1 (Oxoglutarate/iron-dependent oxygenase)

HSP 1 Score: 241.5 bits (615), Expect = 7.0e-64
Identity = 135/312 (43.27%), Postives = 184/312 (58.97%), Query Frame = 0


Query: 7   FLLLLAAAFSFSSCLAQSNSISGRKGLRDQMVNS----GHLSYSNHSERIDPSRVVQISW 66
           FL+L+    S S       S   RK LRD+ + S       SY   S+ +DP+RV+Q+SW
Sbjct: 8   FLILMITMSSSSPPFCSGGS---RKELRDKEITSKSDDTQASYVLGSKFVDPTRVLQLSW 67

Query: 67  QPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDDII 126
            PR FLY+GFLS+EECDHLI+L   + +  S +  G                    D ++
Sbjct: 68  LPRVFLYRGFLSEEECDHLISLRKETTEVYSVDADGK----------------TQLDPVV 127

Query: 127 ARIENRIAVWTFLPKDHSMPFQIMQYGGEEAAGHKYFFGNRSAMPSSEPLMATVVLYLSD 186
           A IE +++ WTFLP ++    ++  Y  E++     +FG   +    E L+ATVVLYLS+
Sbjct: 128 AGIEEKVSAWTFLPGENGGSIKVRSYTSEKSGKKLDYFGEEPSSVLHESLLATVVLYLSN 187

Query: 187 SASGGEILFPVSKVKRRFWSDQRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTPIL 246
           +  GGE+LFP S++K +  +   +  N LRPVKGNA+LFF+  LNAS D    H R P++
Sbjct: 188 TTQGGELLFPNSEMKPK--NSCLEGGNILRPVKGNAILFFTRLLNASLDGKSTHLRCPVV 247

Query: 247 DGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGSPD 306
            G+L VATK  Y +  A       ESG   +C DEDE+C +WA +GECK+N V+MIGSPD
Sbjct: 248 KGELLVATKLIYAKKQA----RIEESG---ECSDEDENCGRWAKLGECKKNPVYMIGSPD 291

Query: 307 YYGTCRKSCNAC 315
           YYGTCRKSCNAC
Sbjct: 308 YYGTCRKSCNAC 291

[Close]

BLAST of Carg23177 vs. TAIR10
Match: AT3G28490.1 (Oxoglutarate/iron-dependent oxygenase)

HSP 1 Score: 213.4 bits (542), Expect = 2.0e-55
Identity = 109/277 (39.35%), Postives = 168/277 (60.65%), Query Frame = 0


Query: 46  SNHSERIDPSRVVQISWQPRAFLYKGFLSDEECDHLIALASNSEDKPS-RNNAGSRNTVS 105
           S+ S  +DP+R+ Q+SW PRAFLYKGFLSDEECDHLI LA    +K     +  S  +  
Sbjct: 21  SSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESED 80

Query: 106 TKFLGNSGAIL-NTTDDIIARIENRIAVWTFLPKDHSMPFQIMQY-GGEEAAGHKYFFGN 165
           ++   +SG  L    DDI+A +E ++A WTFLP+++    QI+ Y  G++   H  +F +
Sbjct: 81  SEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFYD 140

Query: 166 RSAMPSSEPLMATVVLYLSDSASGGEILFP-----VSKVKRRFWSDQRKKNNFLRPVKGN 225
           + A+      +ATV++YLS+   GGE +FP       ++K   WS   K+   ++P KG+
Sbjct: 141 KKALELGGHRIATVLMYLSNVTKGGETVFPNWKGKTPQLKDDSWSKCAKQGYAVKPRKGD 200

Query: 226 AVLFFSVHLNASPDKSCYHSRTPILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDE 285
           A+LFF++HLN + D +  H   P+++G+ W AT++ ++R  + G +  V       C+D+
Sbjct: 201 ALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVR--SFGKKKLV-------CVDD 260

Query: 286 DESCPKWAAIGECKRNAVFMIGSPDYYGTCRKSCNAC 315
            ESC +WA  GEC++N ++M+GS    G CRKSC AC
Sbjct: 261 HESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

[Close]

BLAST of Carg23177 vs. TAIR10
Match: AT3G28480.2 (Oxoglutarate/iron-dependent oxygenase)

HSP 1 Score: 204.1 bits (518), Expect = 1.2e-52
Identity = 119/334 (35.63%), Postives = 185/334 (55.39%), Query Frame = 0


Query: 1   MDSRLNFLLLLAAAFSFSSCLAQSNSISGRKGLRDQMVNSG---HLSYSNHSERIDPSRV 60
           MDSR    + LA +  F   L   +S   R   R      G    +  S  S   DP+RV
Sbjct: 1   MDSR----IFLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIKMKTSASSFGFDPTRV 60

Query: 61  VQISWQPRAFLYKGFLSDEECDHLIALA------SNSEDKPSRNNAGSRNTV-----STK 120
            Q+SW PR FLY+GFLSDEECDH I LA      S   D  S  +  S ++V     S+ 
Sbjct: 61  TQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEDSVSVVRQSSS 120

Query: 121 FLGNSGAILNTTDDIIARIENRIAVWTFLPKDHSMPFQIMQY-GGEEAAGHKYFFGNRSA 180
           F+ N  ++    DDI++ +E ++A WTFLP+++    QI+ Y  G++   H  +F +++ 
Sbjct: 121 FIANMDSL--EIDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQAN 180

Query: 181 MPSSEPLMATVVLYLSDSASGGEILFPV-----SKVKRRFWSDQRKKNNFLRPVKGNAVL 240
           +      +ATV++YLS+   GGE +FP+     +++K   W++  K+   ++P KG+A+L
Sbjct: 181 LELGGHRIATVLMYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALL 240

Query: 241 FFSVHLNASPDKSCYHSRTPILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDES 300
           FF++H NA+ D +  H   P+++G+ W AT++ +++      E A        C+DE+ S
Sbjct: 241 FFNLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVK----SFERAFNK--QSGCMDENVS 300

Query: 301 CPKWAAIGECKRNAVFMIGSPDYYGTCRKSCNAC 315
           C KWA  GEC++N  +M+GS   +G CRKSC AC
Sbjct: 301 CEKWAKAGECQKNPTYMVGSDKDHGYCRKSCKAC 322

[Close]

BLAST of Carg23177 vs. TAIR10
Match: AT3G06300.1 (P4H isoform 2)

HSP 1 Score: 193.7 bits (491), Expect = 1.7e-49
Identity = 104/288 (36.11%), Postives = 166/288 (57.64%), Query Frame = 0


Query: 37  MVNSGHLSYSNHSERIDPSRVVQISWQPRAFLYKGFLSDEECDHLIALA-SNSEDKPSRN 96
           ++ S     S+ S  I+PS+V Q+S +PRAF+Y+GFL+D ECDHLI+LA  N +     +
Sbjct: 18  LLQSSTCLISSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVAD 77

Query: 97  NAGSRNTVSTKFLGNSGAILNTTDDIIARIENRIAVWTFLPKDHSMPFQIMQY-GGEEAA 156
           N    + VS     +   I    D I++ IE++++ WTFLPK++    Q+++Y  G++  
Sbjct: 78  NDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYD 137

Query: 157 GHKYFFGNRSAMPSSEPLMATVVLYLSDSASGGEILFP-VSKVKRRFWSDQR-------K 216
            H  +F ++  +      +ATV+LYLS+   GGE +FP   +  RR  S+ +       K
Sbjct: 138 AHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAK 197

Query: 217 KNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTPILDGKLWVATKFFYIRPAATGNEHAV 276
           K   ++P KGNA+LFF++  +A PD    H   P+++G+ W ATK+ ++        H  
Sbjct: 198 KGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHVDSFDKILTH-- 257

Query: 277 ESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGSPDYYGTCRKSCNAC 315
               D +C D +ESC +WA +GEC +N  +M+G+P+  G CR+SC AC
Sbjct: 258 ----DGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]

BLAST of Carg23177 vs. TAIR10
Match: AT5G18900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 185.7 bits (470), Expect = 4.5e-47
Identity = 96/279 (34.41%), Postives = 169/279 (60.57%), Query Frame = 0


Query: 46  SNHSERIDPSRVVQISWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVST 105
           S+ S  ++PS+V Q+S +PRAF+Y+GFL++ ECDH+++LA  S  + +  +  S  +  +
Sbjct: 26  SSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKFS 85

Query: 106 KFLGNSGAILNT-TDDIIARIENRIAVWTFLPKDHSMPFQIMQY-GGEEAAGHKYFFGNR 165
           +   +SG  ++   D I++ IE++I+ WTFLPK++    Q+++Y  G++   H  +F ++
Sbjct: 86  EVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDK 145

Query: 166 SAMPSSEPLMATVVLYLSDSASGGEILFPVSKVKRR--------FWSDQRKKNNFLRPVK 225
             +      MAT+++YLS+   GGE +FP +++  R          SD  K+   ++P K
Sbjct: 146 VNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKRGIAVKPRK 205

Query: 226 GNAVLFFSVHLNASPDKSCYHSRTPILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCI 285
           G+A+LFF++H +A PD    H   P+++G+ W ATK+ ++    + +     SG   +C 
Sbjct: 206 GDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHV---DSFDRIVTPSG---NCT 265

Query: 286 DEDESCPKWAAIGECKRNAVFMIGSPDYYGTCRKSCNAC 315
           D +ESC +WA +GEC +N  +M+G+ +  G CR+SC AC
Sbjct: 266 DMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

[Close]

BLAST of Carg23177 vs. Swiss-Prot
Match: sp|Q8GXT7|P4H12_ARATH (Probable prolyl 4-hydroxylase 12 OS=Arabidopsis thaliana OX=3702 GN=P4H12 PE=2 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.3e-62
Identity = 135/312 (43.27%), Postives = 184/312 (58.97%), Query Frame = 0


Query: 7   FLLLLAAAFSFSSCLAQSNSISGRKGLRDQMVNS----GHLSYSNHSERIDPSRVVQISW 66
           FL+L+    S S       S   RK LRD+ + S       SY   S+ +DP+RV+Q+SW
Sbjct: 8   FLILMITMSSSSPPFCSGGS---RKELRDKEITSKSDDTQASYVLGSKFVDPTRVLQLSW 67

Query: 67  QPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDDII 126
            PR FLY+GFLS+EECDHLI+L   + +  S +  G                    D ++
Sbjct: 68  LPRVFLYRGFLSEEECDHLISLRKETTEVYSVDADGK----------------TQLDPVV 127

Query: 127 ARIENRIAVWTFLPKDHSMPFQIMQYGGEEAAGHKYFFGNRSAMPSSEPLMATVVLYLSD 186
           A IE +++ WTFLP ++    ++  Y  E++     +FG   +    E L+ATVVLYLS+
Sbjct: 128 AGIEEKVSAWTFLPGENGGSIKVRSYTSEKSGKKLDYFGEEPSSVLHESLLATVVLYLSN 187

Query: 187 SASGGEILFPVSKVKRRFWSDQRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTPIL 246
           +  GGE+LFP S++K +  +   +  N LRPVKGNA+LFF+  LNAS D    H R P++
Sbjct: 188 TTQGGELLFPNSEMKPK--NSCLEGGNILRPVKGNAILFFTRLLNASLDGKSTHLRCPVV 247

Query: 247 DGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGSPD 306
            G+L VATK  Y +  A       ESG   +C DEDE+C +WA +GECK+N V+MIGSPD
Sbjct: 248 KGELLVATKLIYAKKQA----RIEESG---ECSDEDENCGRWAKLGECKKNPVYMIGSPD 291

Query: 307 YYGTCRKSCNAC 315
           YYGTCRKSCNAC
Sbjct: 308 YYGTCRKSCNAC 291

[Close]

BLAST of Carg23177 vs. Swiss-Prot
Match: sp|F4J0A8|P4H6_ARATH (Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 3.7e-54
Identity = 109/277 (39.35%), Postives = 168/277 (60.65%), Query Frame = 0


Query: 46  SNHSERIDPSRVVQISWQPRAFLYKGFLSDEECDHLIALASNSEDKPS-RNNAGSRNTVS 105
           S+ S  +DP+R+ Q+SW PRAFLYKGFLSDEECDHLI LA    +K     +  S  +  
Sbjct: 21  SSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESED 80

Query: 106 TKFLGNSGAIL-NTTDDIIARIENRIAVWTFLPKDHSMPFQIMQY-GGEEAAGHKYFFGN 165
           ++   +SG  L    DDI+A +E ++A WTFLP+++    QI+ Y  G++   H  +F +
Sbjct: 81  SEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFYD 140

Query: 166 RSAMPSSEPLMATVVLYLSDSASGGEILFP-----VSKVKRRFWSDQRKKNNFLRPVKGN 225
           + A+      +ATV++YLS+   GGE +FP       ++K   WS   K+   ++P KG+
Sbjct: 141 KKALELGGHRIATVLMYLSNVTKGGETVFPNWKGKTPQLKDDSWSKCAKQGYAVKPRKGD 200

Query: 226 AVLFFSVHLNASPDKSCYHSRTPILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDE 285
           A+LFF++HLN + D +  H   P+++G+ W AT++ ++R  + G +  V       C+D+
Sbjct: 201 ALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVR--SFGKKKLV-------CVDD 260

Query: 286 DESCPKWAAIGECKRNAVFMIGSPDYYGTCRKSCNAC 315
            ESC +WA  GEC++N ++M+GS    G CRKSC AC
Sbjct: 261 HESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

[Close]

BLAST of Carg23177 vs. Swiss-Prot
Match: sp|Q8L970|P4H7_ARATH (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 6.9e-53
Identity = 117/324 (36.11%), Postives = 183/324 (56.48%), Query Frame = 0


Query: 1   MDSRLNFLLLLAAAFSFSSCLAQSNSISGRKGLRDQMVNSG---HLSYSNHSERIDPSRV 60
           MDSR    + LA +  F   L   +S   R   R      G    +  S  S   DP+RV
Sbjct: 1   MDSR----IFLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIKMKTSASSFGFDPTRV 60

Query: 61  VQISWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILN- 120
            Q+SW PR FLY+GFLSDEECDH I LA    +K    +  S  +V ++   +SG  L+ 
Sbjct: 61  TQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSK 120

Query: 121 TTDDIIARIENRIAVWTFLPKDHSMPFQIMQY-GGEEAAGHKYFFGNRSAMPSSEPLMAT 180
             DDI++ +E ++A WTFLP+++    QI+ Y  G++   H  +F +++ +      +AT
Sbjct: 121 RQDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIAT 180

Query: 181 VVLYLSDSASGGEILFPV-----SKVKRRFWSDQRKKNNFLRPVKGNAVLFFSVHLNASP 240
           V++YLS+   GGE +FP+     +++K   W++  K+   ++P KG+A+LFF++H NA+ 
Sbjct: 181 VLMYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHPNATT 240

Query: 241 DKSCYHSRTPILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGEC 300
           D +  H   P+++G+ W AT++ +++      E A        C+DE+ SC KWA  GEC
Sbjct: 241 DSNSLHGSCPVVEGEKWSATRWIHVK----SFERAFNK--QSGCMDENVSCEKWAKAGEC 300

Query: 301 KRNAVFMIGSPDYYGTCRKSCNAC 315
           ++N  +M+GS   +G CRKSC AC
Sbjct: 301 QKNPTYMVGSDKDHGYCRKSCKAC 314

[Close]

BLAST of Carg23177 vs. Swiss-Prot
Match: sp|F4JAU3|P4H2_ARATH (Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 3.0e-48
Identity = 104/288 (36.11%), Postives = 166/288 (57.64%), Query Frame = 0


Query: 37  MVNSGHLSYSNHSERIDPSRVVQISWQPRAFLYKGFLSDEECDHLIALA-SNSEDKPSRN 96
           ++ S     S+ S  I+PS+V Q+S +PRAF+Y+GFL+D ECDHLI+LA  N +     +
Sbjct: 18  LLQSSTCLISSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVAD 77

Query: 97  NAGSRNTVSTKFLGNSGAILNTTDDIIARIENRIAVWTFLPKDHSMPFQIMQY-GGEEAA 156
           N    + VS     +   I    D I++ IE++++ WTFLPK++    Q+++Y  G++  
Sbjct: 78  NDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYD 137

Query: 157 GHKYFFGNRSAMPSSEPLMATVVLYLSDSASGGEILFP-VSKVKRRFWSDQR-------K 216
            H  +F ++  +      +ATV+LYLS+   GGE +FP   +  RR  S+ +       K
Sbjct: 138 AHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAK 197

Query: 217 KNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTPILDGKLWVATKFFYIRPAATGNEHAV 276
           K   ++P KGNA+LFF++  +A PD    H   P+++G+ W ATK+ ++        H  
Sbjct: 198 KGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHVDSFDKILTH-- 257

Query: 277 ESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGSPDYYGTCRKSCNAC 315
               D +C D +ESC +WA +GEC +N  +M+G+P+  G CR+SC AC
Sbjct: 258 ----DGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]

BLAST of Carg23177 vs. Swiss-Prot
Match: sp|Q8LAN3|P4H4_ARATH (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 8.2e-46
Identity = 96/279 (34.41%), Postives = 169/279 (60.57%), Query Frame = 0


Query: 46  SNHSERIDPSRVVQISWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVST 105
           S+ S  ++PS+V Q+S +PRAF+Y+GFL++ ECDH+++LA  S  + +  +  S  +  +
Sbjct: 26  SSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKFS 85

Query: 106 KFLGNSGAILNT-TDDIIARIENRIAVWTFLPKDHSMPFQIMQY-GGEEAAGHKYFFGNR 165
           +   +SG  ++   D I++ IE++I+ WTFLPK++    Q+++Y  G++   H  +F ++
Sbjct: 86  EVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDK 145

Query: 166 SAMPSSEPLMATVVLYLSDSASGGEILFPVSKVKRR--------FWSDQRKKNNFLRPVK 225
             +      MAT+++YLS+   GGE +FP +++  R          SD  K+   ++P K
Sbjct: 146 VNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKRGIAVKPRK 205

Query: 226 GNAVLFFSVHLNASPDKSCYHSRTPILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCI 285
           G+A+LFF++H +A PD    H   P+++G+ W ATK+ ++    + +     SG   +C 
Sbjct: 206 GDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHV---DSFDRIVTPSG---NCT 265

Query: 286 DEDESCPKWAAIGECKRNAVFMIGSPDYYGTCRKSCNAC 315
           D +ESC +WA +GEC +N  +M+G+ +  G CR+SC AC
Sbjct: 266 DMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

[Close]

BLAST of Carg23177 vs. TrEMBL
Match: tr|A0A1S3AT39|A0A1S3AT39_CUCME (probable prolyl 4-hydroxylase 12 OS=Cucumis melo OX=3656 GN=LOC103482556 PE=4 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 1.3e-141
Identity = 254/315 (80.63%), Postives = 278/315 (88.25%), Query Frame = 0


Query: 1   MDSRLNFLLLLAAAFSFSSCLAQSNSISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQI 60
           MDSRLNFLLL A AFSFS+CLAQSN ISGRKGLRDQ+V+   LSYSN S RIDPSRVVQ+
Sbjct: 1   MDSRLNFLLLFATAFSFSTCLAQSNLISGRKGLRDQLVDR-PLSYSNQSVRIDPSRVVQV 60

Query: 61  SWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDD 120
           SW+PR FLYKGFLSDEECDHLI+LASNSED PSRN+AGS NTVST+ L  SG ILNTTDD
Sbjct: 61  SWRPRVFLYKGFLSDEECDHLISLASNSEDNPSRNSAGSGNTVSTELLNGSGVILNTTDD 120

Query: 121 IIARIENRIAVWTFLPKDHSMPFQIMQYGGEEAAGHKYFFGNRSAM-PSSEPLMATVVLY 180
           IIARIENRIAVWT LPKDH MPFQIMQY GEEA  HKYF+GNRSAM  SSEPLMATVVLY
Sbjct: 121 IIARIENRIAVWTLLPKDHGMPFQIMQYRGEEAK-HKYFYGNRSAMSSSSEPLMATVVLY 180

Query: 181 LSDSASGGEILFPVSKVKRRFWSDQRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRT 240
           LSDSASGGE+LFP SKVK +FWS +RKK NFLRPVKGNA+LFFSVHLNASPDKS YH R 
Sbjct: 181 LSDSASGGEMLFPESKVKSKFWSGRRKKKNFLRPVKGNAILFFSVHLNASPDKSSYHIRY 240

Query: 241 PILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIG 300
           PI +G+LWVATKF Y+RP  TGN+H ++S + D CIDED+SCP+WAAIGEC+RNAVFM+G
Sbjct: 241 PIRNGELWVATKFLYLRP-PTGNKHTIDSNI-DGCIDEDKSCPQWAAIGECERNAVFMVG 300

Query: 301 SPDYYGTCRKSCNAC 315
           SPDYYGTCRKSCNAC
Sbjct: 301 SPDYYGTCRKSCNAC 311

[Close]

BLAST of Carg23177 vs. TrEMBL
Match: tr|A0A0A0KPE4|A0A0A0KPE4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166460 PE=4 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 3.6e-131
Identity = 233/293 (79.52%), Postives = 261/293 (89.08%), Query Frame = 0


Query: 23  QSNSISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQISWQPRAFLYKGFLSDEECDHLI 82
           +SN ISGRKGLRD++V+   LSYSN+S RIDPSRVVQ+SW+PR FLYKGFLSDEECDHLI
Sbjct: 6   KSNLISGRKGLRDRLVDR-PLSYSNYSGRIDPSRVVQVSWRPRVFLYKGFLSDEECDHLI 65

Query: 83  ALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDDIIARIENRIAVWTFLPKDHSMP 142
           +LASNSED PSRN+AGS  TVST+ L +SG ILNTTDDI+ARIENR+A+WT LPKDHSMP
Sbjct: 66  SLASNSEDNPSRNSAGSGITVSTELLNSSGVILNTTDDIVARIENRLAIWTLLPKDHSMP 125

Query: 143 FQIMQYGGEEAAGHKYFFGNRSAM-PSSEPLMATVVLYLSDSASGGEILFPVSKVKRRFW 202
           FQIMQY GEEA  HKYF+GNRSAM PSSEPLMATVVLYLSDSASGGEILFP SKVK +FW
Sbjct: 126 FQIMQYRGEEAK-HKYFYGNRSAMLPSSEPLMATVVLYLSDSASGGEILFPESKVKSKFW 185

Query: 203 SDQRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTPILDGKLWVATKFFYIRPAATG 262
           S +RKKNNFLRPVKGNA+LFFSVHLNASPDKS YH R+PI DG+LWVATKF Y+ P A G
Sbjct: 186 SGRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHIRSPIRDGELWVATKFLYLGPPA-G 245

Query: 263 NEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGSPDYYGTCRKSCNAC 315
           N+H ++S V D C DED+SCP+WAAIGEC+RNAVFM+GSPDYYGTCRKSCNAC
Sbjct: 246 NKHTIQSDV-DGCFDEDKSCPQWAAIGECERNAVFMVGSPDYYGTCRKSCNAC 294

[Close]

BLAST of Carg23177 vs. TrEMBL
Match: tr|A0A2I4HT83|A0A2I4HT83_9ROSI (probable prolyl 4-hydroxylase 12 isoform X2 OS=Juglans regia OX=51240 GN=LOC109021218 PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 4.0e-90
Identity = 172/313 (54.95%), Postives = 223/313 (71.25%), Query Frame = 0


Query: 6   NFLLLLAAAFSFSSCLAQSN--SISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQISWQ 65
           + LLLL  A SF  C  +S+    SG++  ++ ++  GH   S  S RIDPSRVVQ+SWQ
Sbjct: 6   SILLLLVFASSFLICFTESSRKKFSGKQSNQETVIKLGH---SVDSNRIDPSRVVQLSWQ 65

Query: 66  PRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDDIIA 125
           PR FLYKGFLS EECDHLI+L    + +   NN  S + V+ + L +S   LN  DD+++
Sbjct: 66  PRVFLYKGFLSVEECDHLISLVHGRKKEDLGNNGNSEHVVTNRLLMSSKMHLNIEDDVVS 125

Query: 126 RIENRIAVWTFLPKDHSMPFQIMQYGGEEAAGHKYFFGNRSAMPSSEPLMATVVLYLSDS 185
           RIE+RI+ WTFLPK++S P Q+M YG E+   +  FFGNR  +  +EPLMA +VLYLS+ 
Sbjct: 126 RIEDRISAWTFLPKENSRPLQVMHYGLEKVDRNYNFFGNRDLLGLTEPLMAIIVLYLSNV 185

Query: 186 ASGGEILFPVSKVKRRFWSDQRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTPILD 245
             GGEILFP SK+K   WSD    ++  RP+KGNA+LFF++H NASPDKS  H+R P+L+
Sbjct: 186 TQGGEILFPESKLKNTIWSD-CTGSSIPRPIKGNAILFFTLHPNASPDKSSSHARCPVLE 245

Query: 246 GKLWVATKFFYIRPAATGNEHAVESGVDDD--CIDEDESCPKWAAIGECKRNAVFMIGSP 305
           G++W ATKFF+IR + +G + + ES   DD  CIDE E+CP+WAAIGEC+RN VFMIGSP
Sbjct: 246 GEMWHATKFFHIR-SISGEKVSPESDGSDDTGCIDEAENCPRWAAIGECQRNPVFMIGSP 305

Query: 306 DYYGTCRKSCNAC 315
           DYYGTCRKSCNAC
Sbjct: 306 DYYGTCRKSCNAC 313

[Close]

BLAST of Carg23177 vs. TrEMBL
Match: tr|A0A2I4HTA0|A0A2I4HTA0_9ROSI (probable prolyl 4-hydroxylase 12 isoform X3 OS=Juglans regia OX=51240 GN=LOC109021218 PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 4.0e-90
Identity = 172/312 (55.13%), Postives = 222/312 (71.15%), Query Frame = 0


Query: 6   NFLLLLAAAFSFSSCLAQS-NSISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQISWQP 65
           + LLLL  A SF  C  +S    SG++  ++ ++  GH   S  S RIDPSRVVQ+SWQP
Sbjct: 6   SILLLLVFASSFLICFTESRKKFSGKQSNQETVIKLGH---SVDSNRIDPSRVVQLSWQP 65

Query: 66  RAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDDIIAR 125
           R FLYKGFLS EECDHLI+L    + +   NN  S + V+ + L +S   LN  DD+++R
Sbjct: 66  RVFLYKGFLSVEECDHLISLVHGRKKEDLGNNGNSEHVVTNRLLMSSKMHLNIEDDVVSR 125

Query: 126 IENRIAVWTFLPKDHSMPFQIMQYGGEEAAGHKYFFGNRSAMPSSEPLMATVVLYLSDSA 185
           IE+RI+ WTFLPK++S P Q+M YG E+   +  FFGNR  +  +EPLMA +VLYLS+  
Sbjct: 126 IEDRISAWTFLPKENSRPLQVMHYGLEKVDRNYNFFGNRDLLGLTEPLMAIIVLYLSNVT 185

Query: 186 SGGEILFPVSKVKRRFWSDQRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTPILDG 245
            GGEILFP SK+K   WSD    ++  RP+KGNA+LFF++H NASPDKS  H+R P+L+G
Sbjct: 186 QGGEILFPESKLKNTIWSD-CTGSSIPRPIKGNAILFFTLHPNASPDKSSSHARCPVLEG 245

Query: 246 KLWVATKFFYIRPAATGNEHAVESGVDDD--CIDEDESCPKWAAIGECKRNAVFMIGSPD 305
           ++W ATKFF+IR + +G + + ES   DD  CIDE E+CP+WAAIGEC+RN VFMIGSPD
Sbjct: 246 EMWHATKFFHIR-SISGEKVSPESDGSDDTGCIDEAENCPRWAAIGECQRNPVFMIGSPD 305

Query: 306 YYGTCRKSCNAC 315
           YYGTCRKSCNAC
Sbjct: 306 YYGTCRKSCNAC 312

[Close]

BLAST of Carg23177 vs. TrEMBL
Match: tr|A0A2P4NAB4|A0A2P4NAB4_QUESU (Putative prolyl 4-hydroxylase 12 OS=Quercus suber OX=58331 GN=CFP56_60861 PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 4.0e-90
Identity = 174/317 (54.89%), Postives = 218/317 (68.77%), Query Frame = 0


Query: 1   MDSRLNFLLLLAAAFSFSSCLAQSNSISGRKGLRDQMVNSG---HLSYSNHSERIDPSRV 60
           M S L+ LLLLA   SF S LA+S     RK LRD+  +      L +S HS +IDPSRV
Sbjct: 1   MASLLSILLLLAFTSSFQSLLAES-----RKELRDKEASQETFIQLGHSVHSNKIDPSRV 60

Query: 61  VQISWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNT 120
           VQ+SWQPR FLYKGFLSDEECDHLI LA   ++    N+  S +  + + L +S   L+ 
Sbjct: 61  VQLSWQPRVFLYKGFLSDEECDHLITLAHGMKENGLGNDDNSGHVGTDRLLRSSEIPLDI 120

Query: 121 TDDIIARIENRIAVWTFLPKDHSMPFQIMQYGGEEAAGHKYFFGNRSAMPSSEPLMATVV 180
            DD+++RIE RI+ WTFLPK++S P QIM YG EE      + GN+S +  ++PLMA VV
Sbjct: 121 EDDVVSRIEERISAWTFLPKENSRPLQIMHYGLEEVDKKYNYLGNKSTLELTKPLMAIVV 180

Query: 181 LYLSDSASGGEILFPVSKVKRRFWSDQRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHS 240
           LYLS+   GGEI FP S+VK + WS   K +N LRP+KGNA+LFF+VH NASPD S  H+
Sbjct: 181 LYLSNITQGGEIHFPDSEVKSKIWSGCTKSSNILRPIKGNAILFFTVHPNASPDNSSSHA 240

Query: 241 RTPILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFM 300
           R PI++G++W ATKFF++   +   E         DC DE+ESCPKWAAIGEC+RN V+M
Sbjct: 241 RCPIVEGEMWHATKFFHV--GSISGEKLPLKTDGTDCTDEEESCPKWAAIGECQRNPVYM 300

Query: 301 IGSPDYYGTCRKSCNAC 315
           IGSPDYYGTCRKSCNAC
Sbjct: 301 IGSPDYYGTCRKSCNAC 310

The following BLAST results are available for this feature:

BLAST of Carg23177 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022922237.1	9.8e-181	98.73	probable prolyl 4-hydroxylase 12 [Cucurbita moschata]	[more]
XP_023549812.1	6.3e-180	97.78	probable prolyl 4-hydroxylase 12 [Cucurbita pepo subsp. pepo]	[more]
XP_022973003.1	8.3e-180	98.41	probable prolyl 4-hydroxylase 12 [Cucurbita maxima]	[more]
XP_004152378.1	3.1e-142	80.63	PREDICTED: probable prolyl 4-hydroxylase 12 [Cucumis sativus]	[more]
XP_008436994.1	2.0e-141	80.63	PREDICTED: probable prolyl 4-hydroxylase 12 [Cucumis melo]	[more]

BLAST of Carg23177 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G25600.1	7.0e-64	43.27	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28490.1	2.0e-55	39.35	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28480.2	1.2e-52	35.63	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G06300.1	1.7e-49	36.11	P4H isoform 2	[more]
AT5G18900.1	4.5e-47	34.41	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

BLAST of Carg23177 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q8GXT7\|P4H12_ARATH	1.3e-62	43.27	Probable prolyl 4-hydroxylase 12 OS=Arabidopsis thaliana OX=3702 GN=P4H12 PE=2 S...	[more]
sp\|F4J0A8\|P4H6_ARATH	3.7e-54	39.35	Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=...	[more]
sp\|Q8L970\|P4H7_ARATH	6.9e-53	36.11	Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=...	[more]
sp\|F4JAU3\|P4H2_ARATH	3.0e-48	36.11	Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1	[more]
sp\|Q8LAN3\|P4H4_ARATH	8.2e-46	34.41	Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=...	[more]

BLAST of Carg23177 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3AT39\|A0A1S3AT39_CUCME	1.3e-141	80.63	probable prolyl 4-hydroxylase 12 OS=Cucumis melo OX=3656 GN=LOC103482556 PE=4 SV...	[more]
tr\|A0A0A0KPE4\|A0A0A0KPE4_CUCSA	3.6e-131	79.52	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166460 PE=4 SV=1	[more]
tr\|A0A2I4HT83\|A0A2I4HT83_9ROSI	4.0e-90	54.95	probable prolyl 4-hydroxylase 12 isoform X2 OS=Juglans regia OX=51240 GN=LOC1090...	[more]
tr\|A0A2I4HTA0\|A0A2I4HTA0_9ROSI	4.0e-90	55.13	probable prolyl 4-hydroxylase 12 isoform X3 OS=Juglans regia OX=51240 GN=LOC1090...	[more]
tr\|A0A2P4NAB4\|A0A2P4NAB4_QUESU	4.0e-90	54.89	Putative prolyl 4-hydroxylase 12 OS=Quercus suber OX=58331 GN=CFP56_60861 PE=4 S...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity
GO:0005506	iron ion binding
GO:0031418	L-ascorbic acid binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR005123	Oxoglu/Fe-dep_dioxygenase
IPR006620	Pro_4_hyd_alph
IPR003582	ShKT_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg23177-RA	Carg23177-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003582	ShKT domain	SMART	SM00254	ShkT_1	coord: 273..315 e-value: 3.1E-7 score: 40.1
IPR003582	ShKT domain	PROSITE	PS51670	SHKT	coord: 274..314 score: 8.196
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	p4hc	coord: 64..254 e-value: 1.1E-18 score: 78.0
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF13640	2OG-FeII_Oxy_3	coord: 144..254 e-value: 2.2E-6 score: 28.4
None	No IPR available	GENE3D	G3DSA:2.60.120.620		coord: 56..254 e-value: 4.0E-40 score: 139.8
None	No IPR available	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 8..314
None	No IPR available	PANTHER	PTHR10869:SF102	PROLYL 4-HYDROXYLASE 12-RELATED	coord: 8..314
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..20 score: 5.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg23177	Cla003634	Watermelon (97103) v1	carwmB0539
Carg23177	Csa5G166460	Cucumber (Chinese Long) v2	carcuB0573
Carg23177	MELO3C005478	Melon (DHL92) v3.5.1	carmeB0548
Carg23177	ClCG01G004500	Watermelon (Charleston Gray)	carwcgB0528
Carg23177	CSPI05G07280	Wild cucumber (PI 183967)	carcpiB0579
Carg23177	Cucsa.129510	Cucumber (Gy14) v1	carcgyB0619
Carg23177	CmaCh15G010890	Cucurbita maxima (Rimu)	carcmaB0757
Carg23177	CmoCh15G011490	Cucurbita moschata (Rifu)	carcmoB0735
Carg23177	Lsi09G004730	Bottle gourd (USVL1VR-Ls)	carlsiB493
Carg23177	Cp4.1LG13g02580	Cucurbita pepo (Zucchini)	carcpeB0766
Carg23177	CsGy5G004710	Cucumber (Gy14) v2	carcgybB0549
Carg23177	MELO3C005478.2	Melon (DHL92) v3.6.1	carmedB0554
Carg23177	CsaV3_5G004720	Cucumber (Chinese Long) v3	carcucB0580
Carg23177	Cla97C01G004720	Watermelon (97103) v2	carwmbB0569
Carg23177	Bhi12G000444	Wax gourd	carwgoB0651

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg23177	Silver-seed gourd	carcarB505
Carg23177	Cucurbita maxima (Rimu)	carcmaB0759
Carg23177	Cucurbita moschata (Rifu)	carcmoB0737
Carg23177	Cucurbita pepo (Zucchini)	carcpeB0767
Carg23177	Melon (DHL92) v3.5.1	carmeB0547
Carg23177	Melon (DHL92) v3.6.1	carmedB0553