Cla022568 (gene) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla022568
Type	gene
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	EF hand family protein (AHRD V1 ***- B4G1S0_MAIZE); contains Interpro domain(s) IPR018248 EF hand
Location	Chr8 : 24660530 .. 24660847 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTTGTCCAGATCTCAATTGAAGTTGACGAGGGATGAAGTTAGGGAGATCTTACAAGAGCATGACGTTGATGGAGATGGCTCCCTCACCAAGCATGAAGTGATGCAAGCCTTGAACACTATGGGCGCTATGATGTCGTTCCAGAAAGCTCACTATGGTGTTGGTTATGCTGACAAAGATGGCGATGGCTTCGTCAAAGGTGAGGAAGAGATGGAGAAACTCGTCGACTATGTCATGAGGTTTCAGACCCCAAGGACCCCGAAGGTCGTCCCAAAGCCCGCAAATCATTGTAACGAGGGTAAATCCATTGTGTAA

mRNA sequence

ATGGCGTTGTCCAGATCTCAATTGAAGTTGACGAGGGATGAAGTTAGGGAGATCTTACAAGAGCATGACGTTGATGGAGATGGCTCCCTCACCAAGCATGAAGTGATGCAAGCCTTGAACACTATGGGCGCTATGATGTCGTTCCAGAAAGCTCACTATGGTGTTGGTTATGCTGACAAAGATGGCGATGGCTTCGTCAAAGGTGAGGAAGAGATGGAGAAACTCGTCGACTATGTCATGAGGTTTCAGACCCCAAGGACCCCGAAGGTCGTCCCAAAGCCCGCAAATCATTGTAACGAGGGTAAATCCATTGTGTAA

Coding sequence (CDS)

ATGGCGTTGTCCAGATCTCAATTGAAGTTGACGAGGGATGAAGTTAGGGAGATCTTACAAGAGCATGACGTTGATGGAGATGGCTCCCTCACCAAGCATGAAGTGATGCAAGCCTTGAACACTATGGGCGCTATGATGTCGTTCCAGAAAGCTCACTATGGTGTTGGTTATGCTGACAAAGATGGCGATGGCTTCGTCAAAGGTGAGGAAGAGATGGAGAAACTCGTCGACTATGTCATGAGGTTTCAGACCCCAAGGACCCCGAAGGTCGTCCCAAAGCCCGCAAATCATTGTAACGAGGGTAAATCCATTGTGTAA

Protein sequence

MALSRSQLKLTRDEVREILQEHDVDGDGSLTKHEVMQALNTMGAMMSFQKAHYGVGYADKDGDGFVKGEEEMEKLVDYVMRFQTPRTPKVVPKPANHCNEGKSIV

Homology

[Close]

BLAST of Cla022568 vs. TrEMBL
Match: A0A0A0LNG3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357860 PE=4 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 3.5e-37
Identity = 87/110 (79.09%), Postives = 95/110 (86.36%), Query Frame = 1


Query: 1   MALSRSQLKLTRDEVREILQEHDVDGDGSLTKHEVMQALNTMGAMMSFQKAHYGVGYADK 60
           MA+SRSQLKLTRDEVREIL+EHDVDGDGSLTK EVMQALN+MG+MMSFQKAHYGV +ADK
Sbjct: 1   MAMSRSQLKLTRDEVREILEEHDVDGDGSLTKQEVMQALNSMGSMMSFQKAHYGVSHADK 60

Query: 61  DGDGFVK-GEEEMEKLVDYVMRFQTPRTPKV--VPK-PANH-CNEGKSIV 106
           DGDG V  GE EME L+DYVMRFQTPR PK   VPK PA   C++GKSIV
Sbjct: 61  DGDGKVDLGEAEMENLIDYVMRFQTPRAPKTPKVPKVPAQKPCHDGKSIV 110

[Close]

BLAST of Cla022568 vs. TrEMBL
Match: A0A0A0LRC3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359900 PE=4 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 4.6e-29
Identity = 73/97 (75.26%), Postives = 79/97 (81.44%), Query Frame = 1


Query: 1  MALSRSQLKLTRDEVREILQEHDVDGDGSLTKHEVMQALNTMGAMMSFQKAHYGVGYADK 60
          MA SRSQLKLTRDEVREIL+EHDVDGDG LTK EVMQALN+MG MMSFQKAHYGV +ADK
Sbjct: 1  MATSRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMGVMMSFQKAHYGVAFADK 60

Query: 61 DGDGFVKGE-EEMEKLVDYVMRFQTPRTPKVVPKPAN 97
          DGDG V  E  E++ LVDYVMRFQ     K VPK A+
Sbjct: 61 DGDGAVDLEGGEIDSLVDYVMRFQ---ASKDVPKDAS 94

[Close]

BLAST of Cla022568 vs. TrEMBL
Match: A0A0A0LP50_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359910 PE=4 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 4.8e-18
Identity = 50/97 (51.55%), Postives = 74/97 (76.29%), Query Frame = 1


Query: 1  MALSRSQLKLTRDE-VREILQEHDVDGDGSLTKHEVMQALNTMGAMMSFQKAHYGVGYAD 60
          M++SRSQL L+RDE +++I +E+DVDGDG+LTK EVMQA++ MG  + FQ   +G+ +AD
Sbjct: 1  MSVSRSQLNLSRDEMIKKIFEEYDVDGDGTLTKQEVMQAMDAMGFKILFQMTQFGISFAD 60

Query: 61 KDGDGFVKGEEEMEKLVDYVMRFQTPRTPKVVPKPAN 97
          +D DG V  ++E+EKL+D V+RFQT +  +V  +P N
Sbjct: 61 RDDDGKV-DQDELEKLIDCVIRFQTRK--RVCRRPVN 94

[Close]

BLAST of Cla022568 vs. TrEMBL
Match: A0A0A0LRC1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 4.1e-17
Identity = 40/86 (46.51%), Postives = 69/86 (80.23%), Query Frame = 1


Query: 1  MALSRSQLKLTRDEVREILQEHDVDGDGSLTKHEVMQALNTMGAMMSFQKAHYGVGYADK 60
          M+ + ++L+L+R++++EI +EHD+DGDG L+ +E+++A    G+++ F KAHYG+ +AD+
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DGDGFVKGEEEMEKLVDYVMRFQTPR 87
          +GDGF++ EEE++KLVDY  +FQ  R
Sbjct: 61 NGDGFIE-EEELDKLVDYAQKFQHKR 85

[Close]

BLAST of Cla022568 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 4.2e-14
Identity = 37/81 (45.68%), Postives = 59/81 (72.84%), Query Frame = 1


Query: 6  SQLKLTRDEVREILQEHDVDGDGSLTKHEVMQALNTMGAMMSFQKAHYGVGYADKDGDGF 65
          S+   +R+E++EI +EHD+DGDG L+  E+++A+  +G  + F KAHYG+ Y D++GDG 
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 66 VKGEEEMEKLVDYVMRFQTPR 87
          +  E+E++KL+DY  RFQ  R
Sbjct: 67 I-SEDELDKLIDYAERFQKRR 86

[Close]

BLAST of Cla022568 vs. NCBI nr
Match: gi|700207395|gb|KGN62514.1| (hypothetical protein Csa_2G357860 [Cucumis sativus])

HSP 1 Score: 162.2 bits (409), Expect = 5.1e-37
Identity = 87/110 (79.09%), Postives = 95/110 (86.36%), Query Frame = 1


Query: 1   MALSRSQLKLTRDEVREILQEHDVDGDGSLTKHEVMQALNTMGAMMSFQKAHYGVGYADK 60
           MA+SRSQLKLTRDEVREIL+EHDVDGDGSLTK EVMQALN+MG+MMSFQKAHYGV +ADK
Sbjct: 1   MAMSRSQLKLTRDEVREILEEHDVDGDGSLTKQEVMQALNSMGSMMSFQKAHYGVSHADK 60

Query: 61  DGDGFVK-GEEEMEKLVDYVMRFQTPRTPKV--VPK-PANH-CNEGKSIV 106
           DGDG V  GE EME L+DYVMRFQTPR PK   VPK PA   C++GKSIV
Sbjct: 61  DGDGKVDLGEAEMENLIDYVMRFQTPRAPKTPKVPKVPAQKPCHDGKSIV 110

[Close]

BLAST of Cla022568 vs. NCBI nr
Match: gi|700207402|gb|KGN62521.1| (hypothetical protein Csa_2G359900 [Cucumis sativus])

HSP 1 Score: 135.2 bits (339), Expect = 6.7e-29
Identity = 73/97 (75.26%), Postives = 79/97 (81.44%), Query Frame = 1


Query: 1  MALSRSQLKLTRDEVREILQEHDVDGDGSLTKHEVMQALNTMGAMMSFQKAHYGVGYADK 60
          MA SRSQLKLTRDEVREIL+EHDVDGDG LTK EVMQALN+MG MMSFQKAHYGV +ADK
Sbjct: 1  MATSRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMGVMMSFQKAHYGVAFADK 60

Query: 61 DGDGFVKGE-EEMEKLVDYVMRFQTPRTPKVVPKPAN 97
          DGDG V  E  E++ LVDYVMRFQ     K VPK A+
Sbjct: 61 DGDGAVDLEGGEIDSLVDYVMRFQ---ASKDVPKDAS 94

[Close]

BLAST of Cla022568 vs. NCBI nr
Match: gi|700207403|gb|KGN62522.1| (hypothetical protein Csa_2G359910 [Cucumis sativus])

HSP 1 Score: 98.6 bits (244), Expect = 6.9e-18
Identity = 50/97 (51.55%), Postives = 74/97 (76.29%), Query Frame = 1


Query: 1  MALSRSQLKLTRDE-VREILQEHDVDGDGSLTKHEVMQALNTMGAMMSFQKAHYGVGYAD 60
          M++SRSQL L+RDE +++I +E+DVDGDG+LTK EVMQA++ MG  + FQ   +G+ +AD
Sbjct: 1  MSVSRSQLNLSRDEMIKKIFEEYDVDGDGTLTKQEVMQAMDAMGFKILFQMTQFGISFAD 60

Query: 61 KDGDGFVKGEEEMEKLVDYVMRFQTPRTPKVVPKPAN 97
          +D DG V  ++E+EKL+D V+RFQT +  +V  +P N
Sbjct: 61 RDDDGKV-DQDELEKLIDCVIRFQTRK--RVCRRPVN 94

[Close]

BLAST of Cla022568 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 95.9 bits (237), Expect = 4.5e-17
Identity = 40/85 (47.06%), Postives = 68/85 (80.00%), Query Frame = 1


Query: 2   ALSRSQLKLTRDEVREILQEHDVDGDGSLTKHEVMQALNTMGAMMSFQKAHYGVGYADKD 61
           A+  ++L+L+R++++EI +EHD+DGDG L+ +E+++A    G+++ F KAHYG+ +AD++
Sbjct: 83  AIKSTRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADEN 142

Query: 62  GDGFVKGEEEMEKLVDYVMRFQTPR 87
           GDGF++ EEE++KLVDY  +FQ  R
Sbjct: 143 GDGFIE-EEELDKLVDYAQKFQHKR 166

[Close]

BLAST of Cla022568 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 68.6 bits (166), Expect = 7.6e-09
Identity = 31/77 (40.26%), Postives = 52/77 (67.53%), Query Frame = 1


Query: 5  RSQLKLTRDEVREILQEHDVDGDGSLTKHEVMQALNTMGAMMSFQKAHYGVGYADKDGDG 64
          +S  KL ++E++E+ +EHD +GDG L+  E+ +A    GA     +A +G+  AD DGDG
Sbjct: 10 KSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDG 69

Query: 65 FVKGEEEMEKLVDYVMR 82
          F+  E+E++K+VDY ++
Sbjct: 70 FI-SEQELDKVVDYAIK 85

HSP 2 Score: 95.5 bits (236), Expect = 5.8e-17
Identity = 40/86 (46.51%), Postives = 69/86 (80.23%), Query Frame = 1


Query: 1  MALSRSQLKLTRDEVREILQEHDVDGDGSLTKHEVMQALNTMGAMMSFQKAHYGVGYADK 60
          M+ + ++L+L+R++++EI +EHD+DGDG L+ +E+++A    G+++ F KAHYG+ +AD+
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DGDGFVKGEEEMEKLVDYVMRFQTPR 87
          +GDGF++ EEE++KLVDY  +FQ  R
Sbjct: 61 NGDGFIE-EEELDKLVDYAQKFQHKR 85

The following BLAST results are available for this feature:

BLAST of Cla022568 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LNG3_CUCSA	3.5e-37	79.09	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357860 PE=4 SV=1	[more]
A0A0A0LRC3_CUCSA	4.6e-29	75.26	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359900 PE=4 SV=1	[more]
A0A0A0LP50_CUCSA	4.8e-18	51.55	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359910 PE=4 SV=1	[more]
A0A0A0LRC1_CUCSA	4.1e-17	46.51	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1	[more]
A0A0A0LKX1_CUCSA	4.2e-14	45.68	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1	[more]

BLAST of Cla022568 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700207395\|gb\|KGN62514.1\|	5.1e-37	79.09	hypothetical protein Csa_2G357860 [Cucumis sativus]	[more]
gi\|700207402\|gb\|KGN62521.1\|	6.7e-29	75.26	hypothetical protein Csa_2G359900 [Cucumis sativus]	[more]
gi\|700207403\|gb\|KGN62522.1\|	6.9e-18	51.55	hypothetical protein Csa_2G359910 [Cucumis sativus]	[more]
gi\|449470198\|ref\|XP_004152805.1\|	4.5e-17	47.06	PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus]	[more]
gi\|449470198\|ref\|XP_004152805.1\|	7.6e-09	40.26	PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0003674	molecular_function

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
WMU14644	watermelon unigene v2 vs TrEMBL	transcribed_cluster
WMU23828	watermelon unigene v2 vs TrEMBL	transcribed_cluster
WMU34530	watermelon unigene v2 vs TrEMBL	transcribed_cluster
WMU63766	watermelon EST collection version 2.0	transcribed_cluster
WMU63871	watermelon EST collection version 2.0	transcribed_cluster
WMU70853	watermelon EST collection version 2.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla022568	Cla022568.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
WMU23828	WMU23828	transcribed_cluster
WMU34530	WMU34530	transcribed_cluster
WMU14644	WMU14644	transcribed_cluster
WMU63871	WMU63871	transcribed_cluster
WMU70853	WMU70853	transcribed_cluster
WMU63766	WMU63766	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 14..42 score: 2.
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 14..42 score: 0.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 59..82 score: 5.001coord: 10..45 score: 13
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 33..70 score: 2.7E-5coord: 3..32 score: 7.
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 11..76 score: 4.85
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 23..35 scor

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cla022568	Csa2G358890	Cucumber (Chinese Long) v2	cuwmB111
Cla022568	ClCG08G015880	Watermelon (Charleston Gray)	wcgwmB400
Cla022568	CSPI02G19160	Wild cucumber (PI 183967)	cpiwmB116
Cla022568	CsGy2G019080	Cucumber (Gy14) v2	cgybwmB108
Cla022568	CsaV3_2G027860	Cucumber (Chinese Long) v3	cucwmB124
Cla022568	Bhi04G000315	Wax gourd	wgowmB297

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla022568	Watermelon (97103) v1	wmwmB059
Cla022568	Watermelon (97103) v1	wmwmB072
Cla022568	Cucumber (Gy14) v1	cgywmB195
Cla022568	Cucumber (Gy14) v1	cgywmB422
Cla022568	Cucumber (Gy14) v1	cgywmB433
Cla022568	Cucurbita maxima (Rimu)	cmawmB014
Cla022568	Cucurbita maxima (Rimu)	cmawmB148
Cla022568	Cucurbita maxima (Rimu)	cmawmB422
Cla022568	Cucurbita maxima (Rimu)	cmawmB484
Cla022568	Cucurbita maxima (Rimu)	cmawmB753
Cla022568	Cucurbita moschata (Rifu)	cmowmB007
Cla022568	Cucurbita moschata (Rifu)	cmowmB081
Cla022568	Cucurbita moschata (Rifu)	cmowmB134
Cla022568	Cucurbita moschata (Rifu)	cmowmB416
Cla022568	Cucurbita moschata (Rifu)	cmowmB748
Cla022568	Wild cucumber (PI 183967)	cpiwmB300
Cla022568	Wild cucumber (PI 183967)	cpiwmB597
Cla022568	Melon (DHL92) v3.5.1	mewmB218
Cla022568	Melon (DHL92) v3.5.1	mewmB300
Cla022568	Melon (DHL92) v3.5.1	mewmB501
Cla022568	Watermelon (Charleston Gray)	wcgwmB273
Cla022568	Cucumber (Chinese Long) v2	cuwmB298
Cla022568	Cucumber (Chinese Long) v2	cuwmB576
Cla022568	Cucurbita pepo (Zucchini)	cpewmB101
Cla022568	Cucurbita pepo (Zucchini)	cpewmB541
Cla022568	Cucurbita pepo (Zucchini)	cpewmB756
Cla022568	Cucurbita pepo (Zucchini)	cpewmB782
Cla022568	Bottle gourd (USVL1VR-Ls)	lsiwmB293
Cla022568	Bottle gourd (USVL1VR-Ls)	lsiwmB465
Cla022568	Cucumber (Gy14) v2	cgybwmB277
Cla022568	Cucumber (Gy14) v2	cgybwmB539
Cla022568	Melon (DHL92) v3.6.1	medwmB215
Cla022568	Melon (DHL92) v3.6.1	medwmB295
Cla022568	Melon (DHL92) v3.6.1	medwmB487
Cla022568	Silver-seed gourd	carwmB0075
Cla022568	Silver-seed gourd	carwmB0172
Cla022568	Silver-seed gourd	carwmB0766
Cla022568	Cucumber (Chinese Long) v3	cucwmB310
Cla022568	Watermelon (97103) v2	wmwmbB100
Cla022568	Watermelon (97103) v2	wmwmbB091
Cla022568	Wax gourd	wgowmB601