Cla020466 (gene) Watermelon (97103) v1

Overview
NameCla020466
Typegene
OrganismCitrullus. lanatus (Watermelon (97103) v1)
Description3-oxo-5-alpha-steroid 4-dehydrogenase family protein (AHRD V1 ***- Q9LFS3_ARATH); contains Interpro domain(s) IPR001104 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal
LocationChr5 : 29631063 .. 29633274 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGTTTTGTCGGCGTTTCTCTTCCCTCCGCCTCCTTCCACGTTCGTGAACGTGATGACGGTAGTAAGCTCGACCGCGATGGCCATGGCTGGAATTTCCGAGATTAACGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCGAATAGTGTTCCGAAACATTCATCGGAAGAAACTAAGCTTTCGTCCAAATACGGAATGCTCTTGGCTTATACTCCGGCGTTTCTTGCTGGTGCGGCGTCGTTTTGGCTCTTTCCGAGCGGGGATGAGAGGATTCTTCTGCTTAAATCTGCTCTCACTCTCCATTTCTTCAAGAGGATCTTTGAGGTACTCTCTCTTTTTTCCTCTACGCCTCTGATTGAGTTCTCAAATTTCAAATCTGATTCTGGAAATAGGTATAGTAGAATGTAGTTTCTTCTCTGATGTTGATGAATAACTAAATTCATATCCTCGTAAAATTATTTGTTCCAATCGATTGTGGATTTATTGAGAACTTGTTAATCTTATTCTTCGAAAATTTCGCGTGTTCTTGAAAACCGTATGAAAATTCGCGGCGAAGAGAAAATTGTGGAGAGATCTGCATCTCTTCTTCCTTCTCTGCATCCTATCGTTCCTTTGGAGATTTCAAGTTTGGAACTCTTTTCTTCTTTGTTTACGGGACTAGGAAAACTTTTAGCCTAACTTGATTTTCTTAAATTAATTGTTTGTATTATGATTTTTTTTTAGTAGGTGGGGGTTCCGAACTTCGACCTAGCTATGCTCGCAATTCGAGTTTATTAATTTTTCTCTTTGACTAATTGAATTATCATTAAGATACTTTATGAGTCTACTTTGTATAGAATAATCAACATTATAATTATATGAAGTCATGTTTAAGAAAGAAACAATCAACACGATTCTGTTCAACGGTTCTCTAAAAATTCTGTTTCACTAATCACGATATGATTTGCAAGTCTTATACATTTCATTTGTAGTGCTATCATTTTGCAAACTATTCAAGGATTCTTTTCTTTTTCTTGTTTCAACCCGTTGTCGGTGAAGGATTGAACTATGAAAGTTTGAAATGATAATAGATATTTGATAAATTATACGAGCTATTTAGAAAATAATGAAAAATGTTTTAGACAATCAGGTTTGTTGGTTTTAAACAACAAGAAAAACATGGTCTTGTGTTAAATCACCATCAACCTAACAAAGCTTAAGTTTATGGACTATGTTGAATATAGTTATATCAATATTTTATCCTCTTCCTTACTATTAGTAATTTGTAGAAAACTTAACAAGTGGCAATCAATGTTAATATATATATATATTTTAAAAAATACCGAACTTTTTATTAAAGAAATGAAAGAGATTAATCTTCCAAAATACTAAGTAAGTAGAGTCACTACAAATTCAAAAGATAGAGATCAACTAAGAAAGAAACATAAGACTCTTCTAGTTTCTGTTCAAATCTTGGATGGAATAATCCTTAAAACTTTTAGATAATGAACACATGAAGAAGACTTCAAGTGGGTTGAATCAAAGTACTCCAAACATGAGCGTAACGAATTCTGAATTATACGTTGATTTCTCAACAAGTTGTAATCAATGTTAAGTGAAAAGGAAATGACATTAAATAAAAGTTCAAATGCATCAAACTTCTTACTTTAATACCATGTAAATCGAAAATGTACCTCATCGTTTTTTCAAACCATCTCTAAAATAATACTGTTTTTACTCGTGAAAATCTTGACAGGTACTGCTTATTCACAAATACAGCAGTAAAATGGTATTGGAGACAGCAATCAAAATCTCCCTCAGCTACTTCATGTCCACAGCAATCATGATATACACCCAGCACCTTTCACAAGGGATTCCAGAGCCACCCATTGATCTTAAAAACATTGGGATTGGTTTGTTTCTGTTAGGCATTATTGGGAACTTTTACCATCATTATCTCTTGTCCCAAACCAGAAAACAGGGAGAAACAGGCTACAAAATTCCCAAAGGAGGCCTCTTCAGCCTTGTCATTTGCCCTCACTATTTGTTTGAAATAATTGAGTTCTTTGGATTTGCTTTCATCTCTCAGGAACTCTATCCTTTGTGCTTCAGTATTGGCACCAGTATTTATTTGGCTGGGAGGAGCTATGCCACTAGGAAATGGTACATCTCCAAATTTGAGAACTTCCCCAACCATGTCAAAGCCCTGCTCCCTTTTGTCTTTTAG

mRNA sequence

ATGGACGTTTTGTCGGCGTTTCTCTTCCCTCCGCCTCCTTCCACGTTCGTGAACGTGATGACGGTAGTAAGCTCGACCGCGATGGCCATGGCTGGAATTTCCGAGATTAACGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCGAATAGTGTTCCGAAACATTCATCGGAAGAAACTAAGCTTTCGTCCAAATACGGAATGCTCTTGGCTTATACTCCGGCGTTTCTTGCTGGTGCGGCGTCGTTTTGGCTCTTTCCGAGCGGGGATGAGAGGATTCTTCTGCTTAAATCTGCTCTCACTCTCCATTTCTTCAAGAGGATCTTTGAGGTACTCTCTCTTTTTTCCTCTACGCCTCTGATTGAGTTCTCAAATTTCAAATCTGATTCTGGAAATAGCAGTAAAATGGTATTGGAGACAGCAATCAAAATCTCCCTCAGCTACTTCATGTCCACAGCAATCATGATATACACCCAGCACCTTTCACAAGGGATTCCAGAGCCACCCATTGATCTTAAAAACATTGGGATTGGTTTGTTTCTGTTAGGCATTATTGGGAACTTTTACCATCATTATCTCTTGTCCCAAACCAGAAAACAGGGAGAAACAGGCTACAAAATTCCCAAAGGAGGCCTCTTCAGCCTTGTCATTTGCCCTCACTATTTGTTTGAAATAATTGAGTTCTTTGGATTTGCTTTCATCTCTCAGGAACTCTATCCTTTGTGCTTCAGTATTGGCACCAGTATTTATTTGGCTGGGAGGAGCTATGCCACTAGGAAATGGTACATCTCCAAATTTGAGAACTTCCCCAACCATGTCAAAGCCCTGCTCCCTTTTGTCTTTTAG

Coding sequence (CDS)

ATGGACGTTTTGTCGGCGTTTCTCTTCCCTCCGCCTCCTTCCACGTTCGTGAACGTGATGACGGTAGTAAGCTCGACCGCGATGGCCATGGCTGGAATTTCCGAGATTAACGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCGAATAGTGTTCCGAAACATTCATCGGAAGAAACTAAGCTTTCGTCCAAATACGGAATGCTCTTGGCTTATACTCCGGCGTTTCTTGCTGGTGCGGCGTCGTTTTGGCTCTTTCCGAGCGGGGATGAGAGGATTCTTCTGCTTAAATCTGCTCTCACTCTCCATTTCTTCAAGAGGATCTTTGAGGTACTCTCTCTTTTTTCCTCTACGCCTCTGATTGAGTTCTCAAATTTCAAATCTGATTCTGGAAATAGCAGTAAAATGGTATTGGAGACAGCAATCAAAATCTCCCTCAGCTACTTCATGTCCACAGCAATCATGATATACACCCAGCACCTTTCACAAGGGATTCCAGAGCCACCCATTGATCTTAAAAACATTGGGATTGGTTTGTTTCTGTTAGGCATTATTGGGAACTTTTACCATCATTATCTCTTGTCCCAAACCAGAAAACAGGGAGAAACAGGCTACAAAATTCCCAAAGGAGGCCTCTTCAGCCTTGTCATTTGCCCTCACTATTTGTTTGAAATAATTGAGTTCTTTGGATTTGCTTTCATCTCTCAGGAACTCTATCCTTTGTGCTTCAGTATTGGCACCAGTATTTATTTGGCTGGGAGGAGCTATGCCACTAGGAAATGGTACATCTCCAAATTTGAGAACTTCCCCAACCATGTCAAAGCCCTGCTCCCTTTTGTCTTTTAG

Protein sequence

MDVLSAFLFPPPPSTFVNVMTVVSSTAMAMAGISEINGKHLQYSKFWNANSVPKHSSEETKLSSKYGMLLAYTPAFLAGAASFWLFPSGDERILLLKSALTLHFFKRIFEVLSLFSSTPLIEFSNFKSDSGNSSKMVLETAIKISLSYFMSTAIMIYTQHLSQGIPEPPIDLKNIGIGLFLLGIIGNFYHHYLLSQTRKQGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF
Homology
[Close]
BLAST of Cla020466 vs. Swiss-Prot
Match: S5A1_MACFA (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.0e-19
Identity = 45/107 (42.06%), Postives = 65/107 (60.75%), Query Frame = 1 

Query: 175 IGIGLFLLGIIGNFYHHYLLSQTRKQGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFI 234
           IG GL+L G++ N +  ++L   RK G+TGYKIP+GGLF  V   +Y  EI+E+ G+A  
Sbjct: 157 IGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 216

Query: 235 SQELYPLCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 282
           S  +    F+  T  +L+GR+    +WY+ KFE +P   K L+PF+F
Sbjct: 217 SWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPFLF 263

[Close]
BLAST of Cla020466 vs. Swiss-Prot
Match: S5A1_HUMAN (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 5.7e-19
Identity = 44/107 (41.12%), Postives = 65/107 (60.75%), Query Frame = 1 

Query: 175 IGIGLFLLGIIGNFYHHYLLSQTRKQGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFI 234
           IG GL+L G++ N +  ++L   RK G+TGYKIP+GGLF  V   +Y  EI+E+ G+A  
Sbjct: 153 IGFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 212

Query: 235 SQELYPLCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 282
           S  +    F+  T  +L+GR+    +WY+ KFE +P   K ++PF+F
Sbjct: 213 SWSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPFLF 259

[Close]
BLAST of Cla020466 vs. Swiss-Prot
Match: S5A2_MACFA (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis GN=SRD5A2 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 3.1e-17
Identity = 44/107 (41.12%), Postives = 67/107 (62.62%), Query Frame = 1 

Query: 175 IGIGLFLLGIIGNFYHHYLLSQTRKQGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFI 234
           +G+ LF+LG+  N +  Y+L Q RK GE  Y+IPKGGLF+ V   ++L EIIE+ G+A  
Sbjct: 148 LGVFLFILGMGVNIHSDYILRQLRKPGEITYRIPKGGLFTYVSGANFLGEIIEWIGYALA 207

Query: 235 SQELYPLCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 282
           +  L  L F+  +  +L  R++   ++Y+  FE++P   KAL+PF+F
Sbjct: 208 TWSLPALAFAFFSVCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF 254

[Close]
BLAST of Cla020466 vs. Swiss-Prot
Match: S5A1_MOUSE (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus GN=Srd5a1 PE=2 SV=2)

HSP 1 Score: 90.5 bits (223), Expect = 3.1e-17
Identity = 70/255 (27.45%), Postives = 111/255 (43.53%), Query Frame = 1 

Query: 28  MAMAGISEINGKHLQYSKFWNANSVPKHSSEETKLSSKYGMLLAYTPAFLAGAASFWLFP 87
           +A  G+  +   + +YS  W+   VP   +    L     M           AA     P
Sbjct: 22  VAFVGLQMVGSSYGRYSSQWSGRRVPARPA--WFLQELPSMAWPLYECIRPAAARLGNLP 81

Query: 88  SGDERILLLKSALTLHFFKR--IFEVLSLFSSTPLIEFSNFKSDSGNSSKMVLETAIKIS 147
           +   R+LL  +   +H+ +R  +F VL +    P + F+            VL       
Sbjct: 82  N---RVLL--AMFLIHYVQRTLVFPVL-IRGGKPTLLFT-----------FVLAFLFCTL 141

Query: 148 LSYFMSTAIMIYTQHLSQGIPEPPIDLKNIGIGLFLLGIIGNFYHHYLLSQTRKQGETGY 207
             Y  S  +  +  +    +  P       G  L+L+G++ N +  ++L   RK GETGY
Sbjct: 142 NGYLQSRYLSQFAVYAEDWVTHPCF---LTGFALWLVGMVINIHSDHILRNLRKPGETGY 201

Query: 208 KIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSIGTSIYLAGRSYATRKWYISK 267
           KIP+GGLF  V   +Y  E++E+ GFA  S  L  + F++ T   L  R+    +WY+ K
Sbjct: 202 KIPRGGLFEYVSSANYFGELVEWCGFALASWSLQGVVFALFTLCALFTRARQHHQWYLEK 254

Query: 268 FENFPNHVKALLPFV 281
           FE++P   K L+PF+
Sbjct: 262 FEDYPKTRKILIPFL 254

[Close]
BLAST of Cla020466 vs. Swiss-Prot
Match: S5A2_PIG (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa GN=SRD5A2 PE=2 SV=2)

HSP 1 Score: 90.1 bits (222), Expect = 4.1e-17
Identity = 44/108 (40.74%), Postives = 68/108 (62.96%), Query Frame = 1 

Query: 174 NIGIGLFLLGIIGNFYHHYLLSQTRKQGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAF 233
           ++G+ LF+LG+  N +  Y+L Q RK GE  YKIP+GGLF+ V   ++L EIIE+ G+A 
Sbjct: 147 SLGVFLFILGMGINIHSDYILRQLRKPGEVIYKIPQGGLFTYVSGANFLGEIIEWIGYAL 206

Query: 234 ISQELYPLCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 282
            +  L  L F+  +  +L  R++   ++Y+  FE++P   KAL+PF+F
Sbjct: 207 ATWSLPALAFAFFSLCFLGLRAFHHHRFYVKMFEDYPKSRKALIPFIF 254

[Close]
BLAST of Cla020466 vs. TrEMBL
Match: A0A0A0KWK8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612900 PE=4 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 1.5e-103
Identity = 195/282 (69.15%), Postives = 221/282 (78.37%), Query Frame = 1 

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAMAGISEINGKHLQYSKFWNANSVPKHSSEET 60
           MD LS F+FPPP S FVNVMTVVSS  +A+ G+SE  GKHL+YSKFW++N+      +  
Sbjct: 1   MDTLSEFVFPPPASAFVNVMTVVSSAIVAVVGLSEARGKHLKYSKFWSSNNA-NTDRKSF 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERILLLKSALTLHFFKRIFEVLSLFSSTPL 120
            LS+K GML+AY PAFLAG  S WLFPS D RILLLKSAL LHFFKR  EVL L      
Sbjct: 61  NLSAKLGMLVAYIPAFLAGVVSLWLFPSEDRRILLLKSALVLHFFKRNLEVLFLHKY--- 120

Query: 121 IEFSNFKSDSGNSSKMVLETAIKISLSYFMSTAIMIYTQHLSQGI-PEPPIDLKNIGIGL 180
                       SSKM +++ I IS SYF STAIMIYTQHLSQG+  EPPIDLK+IG+GL
Sbjct: 121 ------------SSKMAVDSVITISFSYFSSTAIMIYTQHLSQGLLREPPIDLKSIGVGL 180

Query: 181 FLLGIIGNFYHHYLLSQTRKQGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELY 240
           FL+GIIGNFYHHYLLSQTRKQGET YKIPKGGLFSL+ICPHYLFEIIEFFGFAFISQ +Y
Sbjct: 181 FLIGIIGNFYHHYLLSQTRKQGETSYKIPKGGLFSLIICPHYLFEIIEFFGFAFISQTIY 240

Query: 241 PLCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 282
            L F+  T++YL+GRSYATRKWY+SKFE+FP HVKALLPFVF
Sbjct: 241 SLFFAFATALYLSGRSYATRKWYVSKFEDFPIHVKALLPFVF 266

[Close]
BLAST of Cla020466 vs. TrEMBL
Match: A0A0A0KRA7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612890 PE=4 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.1e-96
Identity = 182/283 (64.31%), Postives = 214/283 (75.62%), Query Frame = 1 

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAMAGISEINGKHLQYSKFWN-ANSVPKHS-SE 60
           M+ LSAF FP PPS  VNVMTVVSS  +A+ G SE  G HLQYSKFWN +NS  K S SE
Sbjct: 7   MNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGTHLQYSKFWNTSNSATKSSNSE 66

Query: 61  ETKLSSKYGMLLAYTPAFLAGAASFWLFPSGDERILLLKSALTLHFFKRIFEVLSLFSST 120
           + ++SS+ GMLL YTPA LAGAASFW  P+ D R+LLLKSALT HFFKR FEVL +    
Sbjct: 67  QLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVLFVHKY- 126

Query: 121 PLIEFSNFKSDSGNSSKMVLETAIKISLSYFMSTAIMIYTQHLSQGIPEPPIDLKNIGIG 180
                         S KMV+++A+ ISLSYF STA+MIY Q  S+ +PEP IDLKNIGI 
Sbjct: 127 --------------SGKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIA 186

Query: 181 LFLLGIIGNFYHHYLLSQTRKQGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQEL 240
           +F++GIIGN YHH LLSQTRK+GETGYKIPKGGLF ++ICPHY+FEI  +FGFA ISQ  
Sbjct: 187 VFVIGIIGNMYHHILLSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTF 246

Query: 241 YPLCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 282
           Y   F+I T+IYL GRSYATRKWY+SKFE+FP H+KAL+PFVF
Sbjct: 247 YAFFFAIATAIYLGGRSYATRKWYVSKFEDFPQHIKALIPFVF 274

[Close]
BLAST of Cla020466 vs. TrEMBL
Match: I1JDE1_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_02G081200 PE=4 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 1.1e-93
Identity = 174/281 (61.92%), Postives = 212/281 (75.44%), Query Frame = 1 

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAMAGISEINGKHLQYSKFWNANSVPKHSSEET 60
           M  L +F+FPPP S  V+ M+V+S  ++A AG SEI GKHL YSKFWNAN     + ++ 
Sbjct: 4   MSALFSFIFPPPSSLVVSGMSVISLVSLANAGFSEIRGKHLNYSKFWNANP---SAEKQV 63

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERILLLKSALTLHFFKRIFEVLSLFSSTPL 120
           KLSSK GMLL YTPAFLAG ASFW+FP    R  LL+SA+TLHFFKR+FEV+ +      
Sbjct: 64  KLSSKAGMLLLYTPAFLAGLASFWIFPHQGLRSTLLQSAVTLHFFKRVFEVVFIHKY--- 123

Query: 121 IEFSNFKSDSGNSSKMVLETAIKISLSYFMSTAIMIYTQHLSQGIPEPPIDLKNIGIGLF 180
                       S  M+L++AI I+LSYF+STA MIY QHL+QG+PEPPIDL   GI LF
Sbjct: 124 ------------SGAMLLDSAIPITLSYFLSTATMIYAQHLTQGLPEPPIDLLYPGIVLF 183

Query: 181 LLGIIGNFYHHYLLSQTRKQGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYP 240
           ++GIIGNFYHHYLLS  R +GE  YKIPKGG+F LVICPHYLFEIIEF+GF+FISQ LY 
Sbjct: 184 VVGIIGNFYHHYLLSNLRGKGEKEYKIPKGGMFELVICPHYLFEIIEFYGFSFISQTLYA 243

Query: 241 LCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 282
             F++GT++YL GRSY+TRKWY+SKFE+FP HVKA++PFVF
Sbjct: 244 FSFTVGTTLYLLGRSYSTRKWYLSKFEDFPEHVKAIIPFVF 266

[Close]
BLAST of Cla020466 vs. TrEMBL
Match: A0A0A0KTC7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612880 PE=4 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 1.4e-93
Identity = 182/282 (64.54%), Postives = 208/282 (73.76%), Query Frame = 1 

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAMAGISEINGKHLQYSKFWNANSVPKHSSEET 60
           MDVLSAFLFPPPPSTFVNVMTVVS+TA+AMAG+SEINGKHLQYSKFWNAN   K S+EE+
Sbjct: 21  MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERILLLKSALTLHFFKRIFEV-LSLFSSTP 120
           K+SSKYGMLLAYTPAFLAGAASFWLFPSGDER+L +    +    K   ++ LS F ST 
Sbjct: 81  KVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVLFIHKYSSKMVLKTAIQISLSYFMSTA 140

Query: 121 LIEFSNFKSDSGNSSKMVLETAIKISLSYFMSTAIMIYTQHLSQGIPEPPIDLKNIGIGL 180
           ++ ++   S       + L+    I +  F+                             
Sbjct: 141 IMIYTQHLSQGLPEPPIDLK---NIGVGSFL----------------------------- 200

Query: 181 FLLGIIGNFYHHYLLSQTRKQGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELY 240
             +GIIGNFYHHYLLSQTRKQG+T YKIPKGGLFS V+CPHYLFEII +FGFAFISQELY
Sbjct: 201 --IGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELY 260

Query: 241 PLCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 282
           PLCFSIGT++YLAGRSYATRKWYISKFE FPN+VKALLPFVF
Sbjct: 261 PLCFSIGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF 268

[Close]
BLAST of Cla020466 vs. TrEMBL
Match: A0A0S3TAT5_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.11G143800 PE=4 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.2e-92
Identity = 172/281 (61.21%), Postives = 209/281 (74.38%), Query Frame = 1 

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAMAGISEINGKHLQYSKFWNANSVPKHSSEET 60
           M    +F+FPPPPS  V+ M+V+S  ++A AG+SEI GKHL YSKFWN N     S ++T
Sbjct: 3   MSTFLSFIFPPPPSLVVSTMSVISLVSLANAGLSEIRGKHLNYSKFWNVNP---SSEKQT 62

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERILLLKSALTLHFFKRIFEVLSLFSSTPL 120
           KLSSK GMLL YTPAFLAG ASFW+FP    R  LL+ A+TLHFFKR FEVL +      
Sbjct: 63  KLSSKAGMLLLYTPAFLAGLASFWIFPHEGLRSTLLQFAVTLHFFKRDFEVLFIHKY--- 122

Query: 121 IEFSNFKSDSGNSSKMVLETAIKISLSYFMSTAIMIYTQHLSQGIPEPPIDLKNIGIGLF 180
                       S  M+L++AI ISLSYF+S A M+Y QHL+QG+PEPPI+L   GI +F
Sbjct: 123 ------------SGGMLLDSAIPISLSYFLSAATMVYAQHLTQGLPEPPINLLYPGIVMF 182

Query: 181 LLGIIGNFYHHYLLSQTRKQGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYP 240
           ++GIIGNFYHHYLLS  R +GE  YKIPKGGLF +VICPHYLFEII+F+G  FISQ LY 
Sbjct: 183 VIGIIGNFYHHYLLSNLRAKGEKEYKIPKGGLFGIVICPHYLFEIIQFYGITFISQTLYG 242

Query: 241 LCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 282
           LCFS+GT+ YL GRSY+TRKWY+SKFE+FP +VKA++PFVF
Sbjct: 243 LCFSLGTTFYLLGRSYSTRKWYLSKFEDFPKNVKAIIPFVF 265

[Close]
BLAST of Cla020466 vs. NCBI nr
Match: gi|778706250|ref|XP_004135052.2| (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis sativus])

HSP 1 Score: 472.2 bits (1214), Expect = 6.1e-130
Identity = 233/281 (82.92%), Postives = 252/281 (89.68%), Query Frame = 1 

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAMAGISEINGKHLQYSKFWNANSVPKHSSEET 60
           MDVLSAFLFPPPPSTFVNVMTVVS+TA+AMAG+SEINGKHLQYSKFWNAN   K S+EE+
Sbjct: 21  MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERILLLKSALTLHFFKRIFEVLSLFSSTPL 120
           K+SSKYGMLLAYTPAFLAGAASFWLFPSGDER+ LLKSALTLHFFKRIFEVL +      
Sbjct: 81  KVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKY--- 140

Query: 121 IEFSNFKSDSGNSSKMVLETAIKISLSYFMSTAIMIYTQHLSQGIPEPPIDLKNIGIGLF 180
                       SSKMVL+TAI+ISLSYFMSTAIMIYTQHLSQG+PEPPIDLKNIG+G F
Sbjct: 141 ------------SSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSF 200

Query: 181 LLGIIGNFYHHYLLSQTRKQGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYP 240
           L+GIIGNFYHHYLLSQTRKQG+T YKIPKGGLFS V+CPHYLFEII +FGFAFISQELYP
Sbjct: 201 LIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYP 260

Query: 241 LCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 282
           LCFSIGT++YLAGRSYATRKWYISKFE FPN+VKALLPFVF
Sbjct: 261 LCFSIGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF 286

[Close]
BLAST of Cla020466 vs. NCBI nr
Match: gi|659091769|ref|XP_008446722.1| (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis melo])

HSP 1 Score: 470.3 bits (1209), Expect = 2.3e-129
Identity = 234/281 (83.27%), Postives = 249/281 (88.61%), Query Frame = 1 

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAMAGISEINGKHLQYSKFWNANSVPKHSSEET 60
           MD+LSAFLFPPPPSTFVNVMTVVSST++AMAG+SEI GKHLQYSKFWNANS  K S++E+
Sbjct: 11  MDILSAFLFPPPPSTFVNVMTVVSSTSLAMAGLSEIKGKHLQYSKFWNANSGSKLSAQES 70

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERILLLKSALTLHFFKRIFEVLSLFSSTPL 120
           KLSSKYGMLL YTPAFLAGAASFWLFPSGDER  LLKSALTLHFFKRIFEVL +      
Sbjct: 71  KLSSKYGMLLVYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKY--- 130

Query: 121 IEFSNFKSDSGNSSKMVLETAIKISLSYFMSTAIMIYTQHLSQGIPEPPIDLKNIGIGLF 180
                       SSKMVL+TAI+IS SYF+STAIMIYTQHLSQG+PEPPIDLKNIG+GLF
Sbjct: 131 ------------SSKMVLKTAIQISFSYFLSTAIMIYTQHLSQGLPEPPIDLKNIGVGLF 190

Query: 181 LLGIIGNFYHHYLLSQTRKQGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYP 240
           L GIIGNFYHHYLLSQTRKQ ET YKIPKGGLFS VICPHYLFEII +FGFAFISQELYP
Sbjct: 191 LTGIIGNFYHHYLLSQTRKQEETAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYP 250

Query: 241 LCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 282
           LCFSIGT+IYLAGRSYATRKWYISKFENFPNHVKALLPFVF
Sbjct: 251 LCFSIGTAIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 276

[Close]
BLAST of Cla020466 vs. NCBI nr
Match: gi|449434532|ref|XP_004135050.1| (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis sativus])

HSP 1 Score: 384.0 bits (985), Expect = 2.2e-103
Identity = 195/282 (69.15%), Postives = 221/282 (78.37%), Query Frame = 1 

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAMAGISEINGKHLQYSKFWNANSVPKHSSEET 60
           MD LS F+FPPP S FVNVMTVVSS  +A+ G+SE  GKHL+YSKFW++N+      +  
Sbjct: 1   MDTLSEFVFPPPASAFVNVMTVVSSAIVAVVGLSEARGKHLKYSKFWSSNNA-NTDRKSF 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERILLLKSALTLHFFKRIFEVLSLFSSTPL 120
            LS+K GML+AY PAFLAG  S WLFPS D RILLLKSAL LHFFKR  EVL L      
Sbjct: 61  NLSAKLGMLVAYIPAFLAGVVSLWLFPSEDRRILLLKSALVLHFFKRNLEVLFLHKY--- 120

Query: 121 IEFSNFKSDSGNSSKMVLETAIKISLSYFMSTAIMIYTQHLSQGI-PEPPIDLKNIGIGL 180
                       SSKM +++ I IS SYF STAIMIYTQHLSQG+  EPPIDLK+IG+GL
Sbjct: 121 ------------SSKMAVDSVITISFSYFSSTAIMIYTQHLSQGLLREPPIDLKSIGVGL 180

Query: 181 FLLGIIGNFYHHYLLSQTRKQGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELY 240
           FL+GIIGNFYHHYLLSQTRKQGET YKIPKGGLFSL+ICPHYLFEIIEFFGFAFISQ +Y
Sbjct: 181 FLIGIIGNFYHHYLLSQTRKQGETSYKIPKGGLFSLIICPHYLFEIIEFFGFAFISQTIY 240

Query: 241 PLCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 282
            L F+  T++YL+GRSYATRKWY+SKFE+FP HVKALLPFVF
Sbjct: 241 SLFFAFATALYLSGRSYATRKWYVSKFEDFPIHVKALLPFVF 266

[Close]
BLAST of Cla020466 vs. NCBI nr
Match: gi|659091773|ref|XP_008446724.1| (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo])

HSP 1 Score: 378.6 bits (971), Expect = 9.2e-102
Identity = 191/282 (67.73%), Postives = 215/282 (76.24%), Query Frame = 1 

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAMAGISEINGKHLQYSKFWNANSVPKHSSEET 60
           MD LS F+FPPP S FVNVMTVVSS  +A+ G+SE  GKHLQYSKFWN N+      +  
Sbjct: 1   MDALSEFVFPPPSSAFVNVMTVVSSAIVAVVGLSEARGKHLQYSKFWNNNNNANTDRKSF 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERILLLKSALTLHFFKRIFEVLSLFSSTPL 120
            LS+K GMLLAY PAFL G  SFWLFP+ D RI++LKSAL LHFFKR  EVL L      
Sbjct: 61  NLSAKLGMLLAYMPAFLVGVVSFWLFPNEDRRIVVLKSALVLHFFKRNLEVLFLHKY--- 120

Query: 121 IEFSNFKSDSGNSSKMVLETAIKISLSYFMSTAIMIYTQHLSQGI-PEPPIDLKNIGIGL 180
                       SSKM + + I IS SYF STAIMIYTQHLSQG+ PEPPIDLK IG+GL
Sbjct: 121 ------------SSKMAIGSVITISFSYFSSTAIMIYTQHLSQGLLPEPPIDLKIIGVGL 180

Query: 181 FLLGIIGNFYHHYLLSQTRKQGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELY 240
           FL+GI+GNFYHHYLLS TRK GET YKIP+GGLF+ VICPHYLFEIIEFFGFAFISQ +Y
Sbjct: 181 FLIGIVGNFYHHYLLSGTRKGGETSYKIPEGGLFNFVICPHYLFEIIEFFGFAFISQTIY 240

Query: 241 PLCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 282
            L F+  T++YL GRSYATRKWY+SKFE+FP HVKALLPFVF
Sbjct: 241 SLFFAFATALYLTGRSYATRKWYVSKFEDFPIHVKALLPFVF 267

[Close]
BLAST of Cla020466 vs. NCBI nr
Match: gi|659091771|ref|XP_008446723.1| (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo])

HSP 1 Score: 366.3 bits (939), Expect = 4.7e-98
Identity = 188/283 (66.43%), Postives = 215/283 (75.97%), Query Frame = 1 

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAMAGISEINGKHLQYSKFWN-ANSVPKHS-SE 60
           M+ LS F FP PPS  VNVMTVVSS  +A+ G+SE  G HLQYSKFWN +NS  K S SE
Sbjct: 1   MNALSVFFFPMPPSLLVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTSNSATKSSNSE 60

Query: 61  ETKLSSKYGMLLAYTPAFLAGAASFWLFPSGDERILLLKSALTLHFFKRIFEVLSLFSST 120
           + ++SS+ GMLL YTPA LAGAASFW FP+ D R+LLLKSALTLHFFKR FEVL +    
Sbjct: 61  QLRISSRLGMLLLYTPAALAGAASFWFFPNDDWRVLLLKSALTLHFFKRDFEVLFVHKY- 120

Query: 121 PLIEFSNFKSDSGNSSKMVLETAIKISLSYFMSTAIMIYTQHLSQGIPEPPIDLKNIGIG 180
                         S KMVL++A  ISLSYF STA+MIY Q LS+ +PEP IDLKNIGI 
Sbjct: 121 --------------SGKMVLDSASIISLSYFSSTALMIYAQQLSKALPEPTIDLKNIGIA 180

Query: 181 LFLLGIIGNFYHHYLLSQTRKQGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQEL 240
           LFL+GIIGN YHH LLSQTRK+GETGYKIPKGGLF +VICPHYLFEI  +FGFA ISQ  
Sbjct: 181 LFLIGIIGNMYHHILLSQTRKKGETGYKIPKGGLFGIVICPHYLFEITVYFGFALISQTF 240

Query: 241 YPLCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 282
           Y   F+I T+IYL GRSYAT KWY+SKFE+FP H+KAL+PFVF
Sbjct: 241 YSFFFAIATAIYLGGRSYATWKWYVSKFEDFPQHIKALIPFVF 268

The following BLAST results are available for this feature:
BLAST of Cla020466 vs. Swiss-Prot
Match NameE-valueIdentityDescription
S5A1_MACFA2.0e-1942.063-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis GN=SRD5A1 PE=1 SV... [more]
S5A1_HUMAN5.7e-1941.123-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens GN=SRD5A1 PE=1 SV=1[more]
S5A2_MACFA3.1e-1741.123-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis GN=SRD5A2 PE=1 SV... [more]
S5A1_MOUSE3.1e-1727.453-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus GN=Srd5a1 PE=2 SV=2[more]
S5A2_PIG4.1e-1740.743-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa GN=SRD5A2 PE=2 SV=2[more]
BLAST of Cla020466 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KWK8_CUCSA1.5e-10369.15Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612900 PE=4 SV=1[more]
A0A0A0KRA7_CUCSA1.1e-9664.31Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612890 PE=4 SV=1[more]
I1JDE1_SOYBN1.1e-9361.92Uncharacterized protein OS=Glycine max GN=GLYMA_02G081200 PE=4 SV=1[more]
A0A0A0KTC7_CUCSA1.4e-9364.54Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612880 PE=4 SV=1[more]
A0A0S3TAT5_PHAAN1.2e-9261.21Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.11G143800 PE=... [more]
BLAST of Cla020466 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778706250|ref|XP_004135052.2|6.1e-13082.92PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis sativus][more]
gi|659091769|ref|XP_008446722.1|2.3e-12983.27PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis melo][more]
gi|449434532|ref|XP_004135050.1|2.2e-10369.15PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis sativus][more]
gi|659091773|ref|XP_008446724.1|9.2e-10267.73PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo][more]
gi|659091771|ref|XP_008446723.1|4.7e-9866.43PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR0011043-oxo-5_a-steroid_4-DH_C
Vocabulary: Molecular Function
TermDefinition
GO:0016627oxidoreductase activity, acting on the CH-CH group of donors
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
GO:0016021integral component of membrane
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors
Unigene
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
WMU19005watermelon EST collection version 2.0transcribed_cluster
WMU36340watermelon unigene v2 vs TrEMBLtranscribed_cluster
WMU45759watermelon EST collection version 2.0transcribed_cluster
WMU64128watermelon EST collection version 2.0transcribed_cluster
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla020466Cla020466.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
WMU45759WMU45759transcribed_cluster
WMU64128WMU64128transcribed_cluster
WMU36340WMU36340transcribed_cluster
WMU19005WMU19005transcribed_cluster


InterPro
Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPFAMPF02544Steroid_dhcoord: 173..281
score: 8.4
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPROFILEPS50244S5A_REDUCTASEcoord: 176..256
score: 25
NoneNo IPR availablePANTHERPTHR105563-OXO-5-ALPHA-STEROID 4-DEHYDROGENASEcoord: 30..281
score: 1.4E
NoneNo IPR availablePANTHERPTHR10556:SF353-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEINcoord: 30..281
score: 1.4E