Cla003201 (gene) Watermelon (97103) v1

NameCla003201
Typegene
OrganismCitrullus. lanatus (Watermelon (97103) v1)
DescriptionFatty acid desaturase (AHRD V1 ***- E5D7U7_9GENT); contains Interpro domain(s) IPR005804 Fatty acid desaturase, type 1
LocationChr2 : 33678795 .. 33679925 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGGTTGGAAGCATGAGAATGGAAAAGAAGATTCATGAGCGAGTTCCCCACGCAAAGCCTCCTTTCACTCTCAGCCAAATCAAGAAAGCCATTCCTCCCCACTGCTTCCACCGCTCTCTTCTCCGTTCCTTCTCCTACTTACTTTCCGACCTCACCATTATCTGTCTCTTCTTCTCCCTCGCCATTACCTATTTCCCGCTTCTCCCCAAACCGCTCCCTTACATTGCATGGCCCTTTTACTGGCTCTTCCAAGGCTGCTTCCTCACCGGCGTCTGGGTCATCGCTCACGAGTGCGGTCACCATGCTTTCTCCGACTACCAATTACTCGACGACATCATCGGCCTCTTCTTCCACTCTGCTCTCCTCGTCCCCTACTTCTCCTGGAAGTACAGTCACCGGCGCCACCACTCCAACACCGCCTCCCTCCAACGGGATGAGGTCTTCGTCCCCAAGCCAAAATCTCAGATCCCTTGGTATCTAAACTACTTCAATAACCTACCAGGGAGGCTCATTTCTCTCCTCGGAACACTCACTCTGGGCTGGCCTTTGTACTTAGCCTTCAACGCCTCAGGCCGTTCCTACAACCGATTCGCCTGCCACTACGATCCCTACGGCCCAATATTCACCAAACGTGAACGGATTCAAATTTACATCTCGGATGCAGGGATTTTAGGGATGGCGTTTGTACTCTACCGAATTGGTGCGGCGAAAGGGGCGTTATGGGTAATTCGTATTTACGGAATGCCGTTGGTGATTGTGAATGGGTTTTTGGTTTTGATTACGTATTTGCAGCATACTCATCCGTCCTTGCCGCACTATGAGTCTTCGGAATGGGATTGGCTGAGAGGGGCTTTAGCAACTGTGGACAGAGACTATGGAGTTCTGAACACAGTGTTTCATAACATAACAGACACCCATGTTGTTCATCATTTGTTCTCTACAATGCCGCATTATCATGCGAAGGAAGCGACGGAAGCGGTGAAGAAAGTATTAGGAGAATATTATCGGATGGATAGGACCAATGTTTTGAAGGCTATTTGGAGGGAGGCTAAGGAGTGTGTTTACGTTGAGGCTGATGAGGATGCCGATTCTGATAAAGGAGTTGTCTGGTACCGAAACAAGCTTTGA

mRNA sequence

ATGGGGGTTGGAAGCATGAGAATGGAAAAGAAGATTCATGAGCGAGTTCCCCACGCAAAGCCTCCTTTCACTCTCAGCCAAATCAAGAAAGCCATTCCTCCCCACTGCTTCCACCGCTCTCTTCTCCGTTCCTTCTCCTACTTACTTTCCGACCTCACCATTATCTGTCTCTTCTTCTCCCTCGCCATTACCTATTTCCCGCTTCTCCCCAAACCGCTCCCTTACATTGCATGGCCCTTTTACTGGCTCTTCCAAGGCTGCTTCCTCACCGGCGTCTGGGTCATCGCTCACGAGTGCGGTCACCATGCTTTCTCCGACTACCAATTACTCGACGACATCATCGGCCTCTTCTTCCACTCTGCTCTCCTCGTCCCCTACTTCTCCTGGAAGTACAGTCACCGGCGCCACCACTCCAACACCGCCTCCCTCCAACGGGATGAGGTCTTCGTCCCCAAGCCAAAATCTCAGATCCCTTGGTATCTAAACTACTTCAATAACCTACCAGGGAGGCTCATTTCTCTCCTCGGAACACTCACTCTGGGCTGGCCTTTGTACTTAGCCTTCAACGCCTCAGGCCGTTCCTACAACCGATTCGCCTGCCACTACGATCCCTACGGCCCAATATTCACCAAACGTGAACGGATTCAAATTTACATCTCGGATGCAGGGATTTTAGGGATGGCGTTTGTACTCTACCGAATTGGTGCGGCGAAAGGGGCGTTATGGGTAATTCGTATTTACGGAATGCCGTTGGTGATTGTGAATGGGTTTTTGGTTTTGATTACGTATTTGCAGCATACTCATCCGTCCTTGCCGCACTATGAGTCTTCGGAATGGGATTGGCTGAGAGGGGCTTTAGCAACTGTGGACAGAGACTATGGAGTTCTGAACACAGTGTTTCATAACATAACAGACACCCATGTTGTTCATCATTTGTTCTCTACAATGCCGCATTATCATGCGAAGGAAGCGACGGAAGCGGTGAAGAAAGTATTAGGAGAATATTATCGGATGGATAGGACCAATGTTTTGAAGGCTATTTGGAGGGAGGCTAAGGAGTGTGTTTACGTTGAGGCTGATGAGGATGCCGATTCTGATAAAGGAGTTGTCTGGTACCGAAACAAGCTTTGA

Coding sequence (CDS)

ATGGGGGTTGGAAGCATGAGAATGGAAAAGAAGATTCATGAGCGAGTTCCCCACGCAAAGCCTCCTTTCACTCTCAGCCAAATCAAGAAAGCCATTCCTCCCCACTGCTTCCACCGCTCTCTTCTCCGTTCCTTCTCCTACTTACTTTCCGACCTCACCATTATCTGTCTCTTCTTCTCCCTCGCCATTACCTATTTCCCGCTTCTCCCCAAACCGCTCCCTTACATTGCATGGCCCTTTTACTGGCTCTTCCAAGGCTGCTTCCTCACCGGCGTCTGGGTCATCGCTCACGAGTGCGGTCACCATGCTTTCTCCGACTACCAATTACTCGACGACATCATCGGCCTCTTCTTCCACTCTGCTCTCCTCGTCCCCTACTTCTCCTGGAAGTACAGTCACCGGCGCCACCACTCCAACACCGCCTCCCTCCAACGGGATGAGGTCTTCGTCCCCAAGCCAAAATCTCAGATCCCTTGGTATCTAAACTACTTCAATAACCTACCAGGGAGGCTCATTTCTCTCCTCGGAACACTCACTCTGGGCTGGCCTTTGTACTTAGCCTTCAACGCCTCAGGCCGTTCCTACAACCGATTCGCCTGCCACTACGATCCCTACGGCCCAATATTCACCAAACGTGAACGGATTCAAATTTACATCTCGGATGCAGGGATTTTAGGGATGGCGTTTGTACTCTACCGAATTGGTGCGGCGAAAGGGGCGTTATGGGTAATTCGTATTTACGGAATGCCGTTGGTGATTGTGAATGGGTTTTTGGTTTTGATTACGTATTTGCAGCATACTCATCCGTCCTTGCCGCACTATGAGTCTTCGGAATGGGATTGGCTGAGAGGGGCTTTAGCAACTGTGGACAGAGACTATGGAGTTCTGAACACAGTGTTTCATAACATAACAGACACCCATGTTGTTCATCATTTGTTCTCTACAATGCCGCATTATCATGCGAAGGAAGCGACGGAAGCGGTGAAGAAAGTATTAGGAGAATATTATCGGATGGATAGGACCAATGTTTTGAAGGCTATTTGGAGGGAGGCTAAGGAGTGTGTTTACGTTGAGGCTGATGAGGATGCCGATTCTGATAAAGGAGTTGTCTGGTACCGAAACAAGCTTTGA

Protein sequence

MGVGSMRMEKKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYFPLLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPYFSWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYLAFNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRIYGMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDTHVVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSDKGVVWYRNKL
BLAST of Cla003201 vs. Swiss-Prot
Match: FAD12_TRIKI (Delta(12)-oleate desaturase OS=Trichosanthes kirilowii GN=FAD2 PE=2 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 7.3e-203
Identity = 326/369 (88.35%), Postives = 340/369 (92.14%), Query Frame = 1

Query: 8   MEKKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYFP 67
           MEK + ERVPHAKPPFTLSQIKK +PPHCFHRSLLRSFSYLLSDL  + LFF LAI YFP
Sbjct: 1   MEKGVQERVPHAKPPFTLSQIKKVVPPHCFHRSLLRSFSYLLSDLAFVSLFFYLAIAYFP 60

Query: 68  LLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPYF 127
           LLP P PYIAWP YW+FQGC LTGVWVIAHECGHHAFSDYQL+DDI+GLFFHSALLVPYF
Sbjct: 61  LLPHPFPYIAWPLYWIFQGCSLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSALLVPYF 120

Query: 128 SWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYLA 187
           SWKYSHRRHHSNT SL+RDEVFVPKPKSQIPWYL YFNN PGRLISL+GTLTLGWPLYLA
Sbjct: 121 SWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYLKYFNNPPGRLISLIGTLTLGWPLYLA 180

Query: 188 FNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRIY 247
           FN SGR Y+RFACHYDPY PIFTKRE IQ+YISDAGILGMAF+LYRIG  KG+ WV+RIY
Sbjct: 181 FNMSGRPYDRFACHYDPYSPIFTKREWIQVYISDAGILGMAFMLYRIGVEKGSFWVMRIY 240

Query: 248 GMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDTH 307
           G+PLVIVNGFLVLITYLQHTHP+LPHYESSEWDWLRGALATVDRDYGVLN VFHNITDTH
Sbjct: 241 GIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTH 300

Query: 308 VVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSDK 367
           VVHHLFSTMPHYHAKEATEAVK VLGEYYR+DRT VLKAIWREAKECVYVE DEDADSDK
Sbjct: 301 VVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDRTPVLKAIWREAKECVYVEPDEDADSDK 360

Query: 368 GVVWYRNKL 377
           GVVWYR KL
Sbjct: 361 GVVWYRKKL 369

BLAST of Cla003201 vs. Swiss-Prot
Match: FAD2_VERFO (Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii GN=FAD2 PE=1 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 3.9e-172
Identity = 272/369 (73.71%), Postives = 310/369 (84.01%), Query Frame = 1

Query: 7   RMEKKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYF 66
           ++E ++ +RVPH+KPPFTL Q+KKAIPPHCF RS+LRSFSY++ DLT+  +F+ +A  YF
Sbjct: 15  KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFSYVVYDLTVAFIFYYIATNYF 74

Query: 67  PLLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPY 126
            LLP+PL Y+AWP YW  QGC LTGVWVIAHECGHHAFSDYQLLDDI+GL  HS LLVPY
Sbjct: 75  HLLPQPLSYVAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQLLDDIVGLVLHSCLLVPY 134

Query: 127 FSWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYL 186
           FSWK+SHRRHHSNTASL+RDEVFVPK KS I W+  Y NN PGRL +L  TLTLGWPLYL
Sbjct: 135 FSWKHSHRRHHSNTASLERDEVFVPKKKSSIRWFSKYLNNPPGRLFTLTITLTLGWPLYL 194

Query: 187 AFNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRI 246
           AFN SGR Y+RFACHYDPYGPI+T RER +IYISDAG+L + F LYR+ AAKG  WVI +
Sbjct: 195 AFNVSGRPYDRFACHYDPYGPIYTDRERTEIYISDAGVLAVTFGLYRLAAAKGLAWVICV 254

Query: 247 YGMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDT 306
           YG+PL+IVN FLV+ITYLQHTHPS+PHY+SSEWDWLRGALATVDRDYG+LN VFHNITDT
Sbjct: 255 YGVPLLIVNAFLVMITYLQHTHPSIPHYDSSEWDWLRGALATVDRDYGILNKVFHNITDT 314

Query: 307 HVVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSD 366
           HV HHLFSTMPHYHA EAT+A+K +LGEYY+ D T   KA+WREAKEC+YVEAD D D  
Sbjct: 315 HVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPFYKAMWREAKECIYVEAD-DGDES 374

Query: 367 KGVVWYRNK 376
           KGV WY  K
Sbjct: 375 KGVYWYNKK 382

BLAST of Cla003201 vs. Swiss-Prot
Match: FAD12_PUNGR (Delta(12)-acyl-lipid-desaturase OS=Punica granatum GN=FAD12 PE=2 SV=2)

HSP 1 Score: 586.3 bits (1510), Expect = 2.4e-166
Identity = 263/375 (70.13%), Postives = 310/375 (82.67%), Query Frame = 1

Query: 2   GVGSMRMEKKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSL 61
           G G  + +K + +RVP AKPPFTLS+IKKAIPPHCF RSLL+SFSY+L DLT++ +F+ +
Sbjct: 15  GEGDEKSQKPV-QRVPSAKPPFTLSEIKKAIPPHCFKRSLLKSFSYVLYDLTLVAIFYYV 74

Query: 62  AITYFPLLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSA 121
           A TY   LP PL Y AWP YW  QGC LTGVWVIAHECGHHAFSDYQ +DD +GL  HSA
Sbjct: 75  ATTYIDALPGPLRYAAWPVYWALQGCVLTGVWVIAHECGHHAFSDYQWVDDCVGLVLHSA 134

Query: 122 LLVPYFSWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLG 181
           LLVPYFSWKYSHRRHHSNT SL+RDEVFVPKPKS++PW+  Y NN PGR+++L+ TLTLG
Sbjct: 135 LLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSKMPWFSKYLNNPPGRVMTLIVTLTLG 194

Query: 182 WPLYLAFNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGAL 241
           WPLYLA N SG  Y+RFACH+DPYGPI+T RER+QIYISD GI+   + LY+I AA+G  
Sbjct: 195 WPLYLALNVSGWPYDRFACHFDPYGPIYTDRERLQIYISDVGIMAATYTLYKIAAARGLA 254

Query: 242 WVIRIYGMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFH 301
           W++ +YG+PL+IVN FLV ITYLQHTHP+LPHY+SSEWDWLRGALAT DRDYG+LN VFH
Sbjct: 255 WLVCVYGVPLLIVNAFLVTITYLQHTHPALPHYDSSEWDWLRGALATADRDYGILNKVFH 314

Query: 302 NITDTHVVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADE 361
           NITDTHV HHLFSTMPHYHA EAT+A+K +LG+YY+ D T V KA+WREA+EC+YVE D+
Sbjct: 315 NITDTHVAHHLFSTMPHYHAMEATKAIKPILGDYYQFDGTPVYKAMWREARECLYVEPDD 374

Query: 362 DADSDKGVVWYRNKL 377
            A+S KGV WY+  L
Sbjct: 375 GANS-KGVFWYKKNL 387

BLAST of Cla003201 vs. Swiss-Prot
Match: FD6E2_SOYBN (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max GN=FAD2-2 PE=2 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 5.6e-163
Identity = 262/363 (72.18%), Postives = 299/363 (82.37%), Query Frame = 1

Query: 14  ERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYFPLLPKPL 73
           +RVP  KP F+LSQIKKAIPPHCF RS+LRSFSY++ DLTI    + +A  YF LLP PL
Sbjct: 22  KRVPFEKPQFSLSQIKKAIPPHCFQRSVLRSFSYVVYDLTIAFCLYYVATHYFHLLPGPL 81

Query: 74  PYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPYFSWKYSH 133
            +     YW  QGC LTGVWVIAHECGHHAFSDYQLLDDI+GL  HSALLVPYFSWKYSH
Sbjct: 82  SFRGMAIYWAVQGCILTGVWVIAHECGHHAFSDYQLLDDIVGLILHSALLVPYFSWKYSH 141

Query: 134 RRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYLAFNASGR 193
           RRHHSNT SL+RDEVFVPK KS I WY  Y NN PGR+++L  TLTLGWPLYLA N SGR
Sbjct: 142 RRHHSNTGSLERDEVFVPKQKSCIKWYSKYLNNPPGRVLTLAVTLTLGWPLYLALNVSGR 201

Query: 194 SYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRIYGMPLVI 253
            Y+RFACHYDPYGPI++ RER+QIYISDAG+L + + L+R+  AKG  WV+ +YG+PL++
Sbjct: 202 PYDRFACHYDPYGPIYSDRERLQIYISDAGVLAVVYGLFRLAMAKGLAWVVCVYGVPLLV 261

Query: 254 VNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDTHVVHHLF 313
           VNGFLVLIT+LQHTHP+LPHY SSEWDWLRGALATVDRDYG+LN VFHNITDTHV HHLF
Sbjct: 262 VNGFLVLITFLQHTHPALPHYTSSEWDWLRGALATVDRDYGILNKVFHNITDTHVAHHLF 321

Query: 314 STMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSDKGVVWYR 373
           STMPHYHA EAT+A+K +LGEYYR D T  +KA+WREA+EC+YVE D+  +S KGV WY 
Sbjct: 322 STMPHYHAMEATKAIKPILGEYYRFDETPFVKAMWREARECIYVEPDQSTES-KGVFWYN 381

Query: 374 NKL 377
           NKL
Sbjct: 382 NKL 383

BLAST of Cla003201 vs. Swiss-Prot
Match: FH12_RICCO (Phosphatidylcholine 12-monooxygenase OS=Ricinus communis GN=FAH12 PE=1 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 7.6e-160
Identity = 252/362 (69.61%), Postives = 294/362 (81.22%), Query Frame = 1

Query: 14  ERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYFPLLPKPL 73
           +R PH KPPFTL  +K+AIPPHCF RS +RSFSY+  D+ +  LF+S+A  +FP +  PL
Sbjct: 26  KRAPHTKPPFTLGDLKRAIPPHCFERSFVRSFSYVAYDVCLSFLFYSIATNFFPYISSPL 85

Query: 74  PYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPYFSWKYSH 133
            Y+AW  YWLFQGC LTG+WVI HECGHHAFS+YQL DDI+GL  HSALLVPYFSWKYSH
Sbjct: 86  SYVAWLVYWLFQGCILTGLWVIGHECGHHAFSEYQLADDIVGLIVHSALLVPYFSWKYSH 145

Query: 134 RRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYLAFNASGR 193
           RRHHSN  SL+RDEVFVPK KS+I WY  Y NN PGR+++L  TL LGWPLYLAFN SGR
Sbjct: 146 RRHHSNIGSLERDEVFVPKSKSKISWYSKYSNNPPGRVLTLAATLLLGWPLYLAFNVSGR 205

Query: 194 SYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRIYGMPLVI 253
            Y+RFACHYDPYGPIF++RER+QIYI+D GI    FVLY+   AKG  WV+RIYG+PL+I
Sbjct: 206 PYDRFACHYDPYGPIFSERERLQIYIADLGIFATTFVLYQATMAKGLAWVMRIYGVPLLI 265

Query: 254 VNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDTHVVHHLF 313
           VN FLV+ITYLQHTHP++P Y SSEWDWLRGA+ TVDRDYGVLN VFHNI DTHV HHLF
Sbjct: 266 VNCFLVMITYLQHTHPAIPRYGSSEWDWLRGAMVTVDRDYGVLNKVFHNIADTHVAHHLF 325

Query: 314 STMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSDKGVVWYR 373
           +T+PHYHA EAT+A+K ++GEYYR D T   KA+WREAKEC++VE DE A + +GV WYR
Sbjct: 326 ATVPHYHAMEATKAIKPIMGEYYRYDGTPFYKALWREAKECLFVEPDEGAPT-QGVFWYR 385

Query: 374 NK 376
           NK
Sbjct: 386 NK 386

BLAST of Cla003201 vs. TrEMBL
Match: W9QMT8_9ROSA (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Morus notabilis GN=L484_018419 PE=4 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 1.1e-170
Identity = 275/363 (75.76%), Postives = 312/363 (85.95%), Query Frame = 1

Query: 14  ERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYFPLLPKPL 73
           ERVPH  PPFTLSQIKKAIPPHCF RSL+RSFSY+  DL    LF+ +A +YF LLP PL
Sbjct: 23  ERVPHTTPPFTLSQIKKAIPPHCFKRSLVRSFSYVGHDLFFAALFYYIATSYFHLLPHPL 82

Query: 74  PYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPYFSWKYSH 133
           PY+AWP YW+ QGC  TG+WVIAHECGHHAFSD+Q +DD +GL  HSALLVPYFSWKYSH
Sbjct: 83  PYLAWPLYWIVQGCVCTGIWVIAHECGHHAFSDHQWVDDTVGLILHSALLVPYFSWKYSH 142

Query: 134 RRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYLAFNASGR 193
           RRHHSNT SL+RDEVFVPKPKS+I WY  Y NN PGRL+SLL TLTLGWPLYLAFN SGR
Sbjct: 143 RRHHSNTGSLERDEVFVPKPKSKISWYSKYLNNPPGRLLSLLVTLTLGWPLYLAFNVSGR 202

Query: 194 SYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRIYGMPLVI 253
            Y+RFACHYDPYGPI++ RER+QI++SDAGI    FVLY++ AAKG  W++ +YG+PL+I
Sbjct: 203 PYDRFACHYDPYGPIYSDRERLQIFVSDAGIFVTTFVLYQLAAAKGLAWIVCVYGVPLLI 262

Query: 254 VNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDTHVVHHLF 313
           VNGFLVLITYLQHTHP+LPHY+SSEWDWLRGALATVDRDYGVLN VFHNI DTHV HHLF
Sbjct: 263 VNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRDYGVLNRVFHNINDTHVAHHLF 322

Query: 314 STMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSDKGVVWYR 373
           STMPHY+A EAT+AVK +LGEYYR DRT + KA+WREAKEC+YVE D+DA  +KGV WYR
Sbjct: 323 STMPHYNAMEATKAVKPLLGEYYRFDRTPIFKAMWREAKECLYVEPDDDA-PNKGVFWYR 382

Query: 374 NKL 377
           NKL
Sbjct: 383 NKL 384

BLAST of Cla003201 vs. TrEMBL
Match: I6LVA4_VERFO (Fad2 OS=Vernicia fordii GN=fad2 PE=4 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 1.3e-169
Identity = 271/369 (73.44%), Postives = 312/369 (84.55%), Query Frame = 1

Query: 7   RMEKKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYF 66
           ++E ++ +RVPH+KPPFTL Q+KKAIPPHCF RS+LRSFSY++ DLT+  +F+ +A  YF
Sbjct: 15  KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFSYVVYDLTVAFIFYYIATNYF 74

Query: 67  PLLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPY 126
            LLP+PL Y+AWP YW  QGC LTGVWVIAHECGHHAFSDYQLLDDI+GL  HS LLVPY
Sbjct: 75  HLLPQPLSYVAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQLLDDIVGLVLHSCLLVPY 134

Query: 127 FSWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYL 186
           FSWK+SHRRHHSNTASL+RDEVFVPK KS I W+  Y NN PGRL +L  TLTLGWPLYL
Sbjct: 135 FSWKHSHRRHHSNTASLERDEVFVPKKKSSIRWFSKYLNNPPGRLFTLTITLTLGWPLYL 194

Query: 187 AFNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRI 246
           AFN SGR Y+RFACHYDPYGPI+T RER +IYISDAG+L + F LYR+ AAKG  WVI +
Sbjct: 195 AFNVSGRPYDRFACHYDPYGPIYTDRERTEIYISDAGVLAVTFGLYRLAAAKGLAWVICV 254

Query: 247 YGMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDT 306
           YG+PL+IVN FLV+ITYLQHTHPS+PHY+SSEWDWLRGALATVDRDYG+LN VFHNITDT
Sbjct: 255 YGVPLLIVNAFLVMITYLQHTHPSIPHYDSSEWDWLRGALATVDRDYGILNKVFHNITDT 314

Query: 307 HVVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSD 366
           HV HHLFSTMPHYHA EA++A+K +LGEYY+ D T   KA+WREAKEC+YVEAD D D  
Sbjct: 315 HVAHHLFSTMPHYHAMEASKAIKPILGEYYQFDGTPFYKAMWREAKECIYVEAD-DGDES 374

Query: 367 KGVVWYRNK 376
           KGV WY  K
Sbjct: 375 KGVYWYNKK 382

BLAST of Cla003201 vs. TrEMBL
Match: I6LVA3_VERFO (Fad2 OS=Vernicia fordii GN=fad2 PE=4 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 3.7e-169
Identity = 271/369 (73.44%), Postives = 311/369 (84.28%), Query Frame = 1

Query: 7   RMEKKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYF 66
           ++E ++ +RVPH+KPPFTL Q+KKAIPPHCF RS+LRSFSY++ DLT+  +F+ +A  YF
Sbjct: 15  KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFSYVVYDLTVAFIFYYIATNYF 74

Query: 67  PLLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPY 126
            LL +PL Y+AWP YW  QGC LTGVWVIAHECGHHAFSDYQLLDDI+GL  HS LLVPY
Sbjct: 75  HLLTQPLSYVAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQLLDDIVGLVLHSCLLVPY 134

Query: 127 FSWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYL 186
           FSWK+SHRRHHSNTASL+RDEVFVPK KS I W+  Y NN PGRL +L  TLTLGWPLYL
Sbjct: 135 FSWKHSHRRHHSNTASLERDEVFVPKKKSSIRWFSKYLNNPPGRLFTLTITLTLGWPLYL 194

Query: 187 AFNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRI 246
           AFN SGR Y+RFACHYDPYGPI+T RER +IYISDAG+L + F LYR+ AAKG  WVI +
Sbjct: 195 AFNVSGRPYDRFACHYDPYGPIYTDRERTEIYISDAGVLAVTFGLYRLAAAKGLAWVICV 254

Query: 247 YGMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDT 306
           YG+PL+IVN FLV+ITYLQHTHPS+PHY+SSEWDWLRGALATVDRDYG+LN VFHNITDT
Sbjct: 255 YGVPLLIVNAFLVMITYLQHTHPSIPHYDSSEWDWLRGALATVDRDYGILNKVFHNITDT 314

Query: 307 HVVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSD 366
           HV HHLFSTMPHYHA EAT+A+K +LGEYY+ D T   KA+WREAKEC+YVEAD D D  
Sbjct: 315 HVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPFYKAMWREAKECIYVEAD-DGDES 374

Query: 367 KGVVWYRNK 376
           KGV WY  K
Sbjct: 375 KGVYWYNKK 382

BLAST of Cla003201 vs. TrEMBL
Match: A0A068VA14_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00011472001 PE=4 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 4.8e-169
Identity = 271/363 (74.66%), Postives = 311/363 (85.67%), Query Frame = 1

Query: 14  ERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYFPLLPKPL 73
           +RVP++KPPFT+  IKKAIPPHCF RSLLRSFSY++ DLT++ LF+ +A TYF LLP P 
Sbjct: 22  KRVPYSKPPFTVGDIKKAIPPHCFKRSLLRSFSYVVYDLTLVSLFYYIATTYFHLLPSPY 81

Query: 74  PYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPYFSWKYSH 133
            Y+AWP YW+FQGC  TGVWVIAHECGHHAFSDYQ +DD +GL  HSALLVPYFSWKYSH
Sbjct: 82  NYLAWPVYWIFQGCVCTGVWVIAHECGHHAFSDYQWVDDTVGLILHSALLVPYFSWKYSH 141

Query: 134 RRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYLAFNASGR 193
           RRHHSNT SL+RDEVFVPKPKS++ WY  Y NN PGR+I+L  TLTLGWPLYLAFNASGR
Sbjct: 142 RRHHSNTGSLERDEVFVPKPKSKMAWYSKYLNNPPGRVITLTITLTLGWPLYLAFNASGR 201

Query: 194 SYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRIYGMPLVI 253
            Y+RFACHYDPYGPI+  RER+QIYISD G++  +++LYR+  AKG  W+I IYG+PL+I
Sbjct: 202 PYDRFACHYDPYGPIYNDRERLQIYISDVGVIATSYLLYRVAMAKGLAWLICIYGVPLLI 261

Query: 254 VNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDTHVVHHLF 313
           VNGFLVLITYLQHTHPSLPHY+SSEWDWLRGALATVDRDYGVLN VFHNITDTHV HHLF
Sbjct: 262 VNGFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRDYGVLNKVFHNITDTHVAHHLF 321

Query: 314 STMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSDKGVVWYR 373
           STMPHYHA EAT+A+K +LGEYY++D T   KA+WREAKEC+YVE DE     KGV WY+
Sbjct: 322 STMPHYHAMEATKAIKPLLGEYYQLDGTPFYKAMWREAKECIYVEPDE---GGKGVFWYK 381

Query: 374 NKL 377
           NKL
Sbjct: 382 NKL 381

BLAST of Cla003201 vs. TrEMBL
Match: U5TMI2_MILPI (Microsomal omega-6-desaturase OS=Millettia pinnata GN=FAD2-2 PE=2 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 6.9e-168
Identity = 269/362 (74.31%), Postives = 307/362 (84.81%), Query Frame = 1

Query: 14  ERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYFPLLPKPL 73
           +RVP++KPPF+L Q+KKAIPPHCF RS+LRSFSY++ DLTI  LF+ +A  YF LLP+PL
Sbjct: 22  KRVPYSKPPFSLGQVKKAIPPHCFQRSVLRSFSYVVYDLTIAFLFYYVATNYFHLLPQPL 81

Query: 74  PYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPYFSWKYSH 133
            Y+AWP YW  QGC LTG+WVIAHECGHHAFSDYQLLDDI+GL  HS LLVPYFSWKYSH
Sbjct: 82  SYVAWPIYWALQGCVLTGIWVIAHECGHHAFSDYQLLDDIVGLILHSCLLVPYFSWKYSH 141

Query: 134 RRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYLAFNASGR 193
           RRHHSNT SL+RDEVFVPK KS I WY  Y NN PGRL +L  TLTLGWPLYLAFN SGR
Sbjct: 142 RRHHSNTGSLERDEVFVPKQKSCIRWYSKYLNNPPGRLFTLTVTLTLGWPLYLAFNVSGR 201

Query: 194 SYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRIYGMPLVI 253
            Y+RFACHYDPYGPI+  RER+QIYISDAG+L + F LYR+  AKG  WV+ +YG+PL+I
Sbjct: 202 PYDRFACHYDPYGPIYNDRERLQIYISDAGVLAVTFGLYRLALAKGLAWVVCVYGVPLLI 261

Query: 254 VNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDTHVVHHLF 313
           VN FLVLITYLQHTHPS+PHY+SSEWDWLRGALATVDRDYG+LN VFHNITDTHV HHLF
Sbjct: 262 VNAFLVLITYLQHTHPSIPHYDSSEWDWLRGALATVDRDYGILNKVFHNITDTHVAHHLF 321

Query: 314 STMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSDKGVVWYR 373
           STMPHYHA EAT+A+K +LGEYY+ D T  +KA+WREAKEC+YVE DE    +KGV WY+
Sbjct: 322 STMPHYHAMEATKAIKPILGEYYQFDGTPFVKAMWREAKECIYVEPDE-GTQNKGVYWYK 381

Query: 374 NK 376
           NK
Sbjct: 382 NK 382

BLAST of Cla003201 vs. NCBI nr
Match: gi|75328134|sp|Q84UB9.1|FAD12_TRIKI (RecName: Full=Delta(12)-oleate desaturase; Short=PgFAD2)

HSP 1 Score: 707.6 bits (1825), Expect = 1.2e-200
Identity = 326/369 (88.35%), Postives = 342/369 (92.68%), Query Frame = 1

Query: 8   MEKKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYFP 67
           MEK + ERVPHAKPPFTLSQIKK +PPHCFHRSLLRSFSYLLSDL  + LFF LAI YFP
Sbjct: 1   MEKGVQERVPHAKPPFTLSQIKKVVPPHCFHRSLLRSFSYLLSDLAFVSLFFYLAIAYFP 60

Query: 68  LLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPYF 127
           LLP P PYIAWP YW+FQGC LTGVWVIAHECGHHAFSDYQL+DDI+GLFFHSALLVPYF
Sbjct: 61  LLPHPFPYIAWPLYWIFQGCSLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSALLVPYF 120

Query: 128 SWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYLA 187
           SWKYSHRRHHSNT SL+RDEVFVPKPKSQIPWYL YFNN PGRLISL+GTLTLGWPLYLA
Sbjct: 121 SWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYLKYFNNPPGRLISLIGTLTLGWPLYLA 180

Query: 188 FNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRIY 247
           FN SGR Y+RFACHYDPY PIFTKRE IQ+YISDAGILGMAF+LYRIG  KG+ WV+RIY
Sbjct: 181 FNMSGRPYDRFACHYDPYSPIFTKREWIQVYISDAGILGMAFMLYRIGVEKGSFWVMRIY 240

Query: 248 GMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDTH 307
           G+PLVIVNGFLVLITYLQHTHP+LPHYESSEWDWLRGALATVDRDYGVLN VFHNITDTH
Sbjct: 241 GIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTH 300

Query: 308 VVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSDK 367
           VVHHLFSTMPHYHAKEATEAVK VLGEYYR+DRT VLKAIWREAKECVYVE DEDADSDK
Sbjct: 301 VVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDRTPVLKAIWREAKECVYVEPDEDADSDK 360

Query: 368 GVVWYRNKL 377
           GVVWYR KL
Sbjct: 361 GVVWYRKKL 369

BLAST of Cla003201 vs. NCBI nr
Match: gi|703065220|ref|XP_010087393.1| (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 [Morus notabilis])

HSP 1 Score: 607.4 bits (1565), Expect = 1.6e-170
Identity = 275/363 (75.76%), Postives = 312/363 (85.95%), Query Frame = 1

Query: 14  ERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYFPLLPKPL 73
           ERVPH  PPFTLSQIKKAIPPHCF RSL+RSFSY+  DL    LF+ +A +YF LLP PL
Sbjct: 23  ERVPHTTPPFTLSQIKKAIPPHCFKRSLVRSFSYVGHDLFFAALFYYIATSYFHLLPHPL 82

Query: 74  PYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPYFSWKYSH 133
           PY+AWP YW+ QGC  TG+WVIAHECGHHAFSD+Q +DD +GL  HSALLVPYFSWKYSH
Sbjct: 83  PYLAWPLYWIVQGCVCTGIWVIAHECGHHAFSDHQWVDDTVGLILHSALLVPYFSWKYSH 142

Query: 134 RRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYLAFNASGR 193
           RRHHSNT SL+RDEVFVPKPKS+I WY  Y NN PGRL+SLL TLTLGWPLYLAFN SGR
Sbjct: 143 RRHHSNTGSLERDEVFVPKPKSKISWYSKYLNNPPGRLLSLLVTLTLGWPLYLAFNVSGR 202

Query: 194 SYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRIYGMPLVI 253
            Y+RFACHYDPYGPI++ RER+QI++SDAGI    FVLY++ AAKG  W++ +YG+PL+I
Sbjct: 203 PYDRFACHYDPYGPIYSDRERLQIFVSDAGIFVTTFVLYQLAAAKGLAWIVCVYGVPLLI 262

Query: 254 VNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDTHVVHHLF 313
           VNGFLVLITYLQHTHP+LPHY+SSEWDWLRGALATVDRDYGVLN VFHNI DTHV HHLF
Sbjct: 263 VNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRDYGVLNRVFHNINDTHVAHHLF 322

Query: 314 STMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSDKGVVWYR 373
           STMPHY+A EAT+AVK +LGEYYR DRT + KA+WREAKEC+YVE D+DA  +KGV WYR
Sbjct: 323 STMPHYNAMEATKAVKPLLGEYYRFDRTPIFKAMWREAKECLYVEPDDDA-PNKGVFWYR 382

Query: 374 NKL 377
           NKL
Sbjct: 383 NKL 384

BLAST of Cla003201 vs. NCBI nr
Match: gi|75329014|sp|Q8GZC3.1|FAD2_VERFO (RecName: Full=Delta(12)-fatty-acid desaturase FAD2)

HSP 1 Score: 605.5 bits (1560), Expect = 6.2e-170
Identity = 272/369 (73.71%), Postives = 312/369 (84.55%), Query Frame = 1

Query: 7   RMEKKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYF 66
           ++E ++ +RVPH+KPPFTL Q+KKAIPPHCF RS+LRSFSY++ DLT+  +F+ +A  YF
Sbjct: 15  KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFSYVVYDLTVAFIFYYIATNYF 74

Query: 67  PLLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPY 126
            LLP+PL Y+AWP YW  QGC LTGVWVIAHECGHHAFSDYQLLDDI+GL  HS LLVPY
Sbjct: 75  HLLPQPLSYVAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQLLDDIVGLVLHSCLLVPY 134

Query: 127 FSWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYL 186
           FSWK+SHRRHHSNTASL+RDEVFVPK KS I W+  Y NN PGRL +L  TLTLGWPLYL
Sbjct: 135 FSWKHSHRRHHSNTASLERDEVFVPKKKSSIRWFSKYLNNPPGRLFTLTITLTLGWPLYL 194

Query: 187 AFNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRI 246
           AFN SGR Y+RFACHYDPYGPI+T RER +IYISDAG+L + F LYR+ AAKG  WVI +
Sbjct: 195 AFNVSGRPYDRFACHYDPYGPIYTDRERTEIYISDAGVLAVTFGLYRLAAAKGLAWVICV 254

Query: 247 YGMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDT 306
           YG+PL+IVN FLV+ITYLQHTHPS+PHY+SSEWDWLRGALATVDRDYG+LN VFHNITDT
Sbjct: 255 YGVPLLIVNAFLVMITYLQHTHPSIPHYDSSEWDWLRGALATVDRDYGILNKVFHNITDT 314

Query: 307 HVVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSD 366
           HV HHLFSTMPHYHA EAT+A+K +LGEYY+ D T   KA+WREAKEC+YVEAD D D  
Sbjct: 315 HVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPFYKAMWREAKECIYVEAD-DGDES 374

Query: 367 KGVVWYRNK 376
           KGV WY  K
Sbjct: 375 KGVYWYNKK 382

BLAST of Cla003201 vs. NCBI nr
Match: gi|332384317|gb|AEE69021.1| (Fad2 [Vernicia fordii])

HSP 1 Score: 604.0 bits (1556), Expect = 1.8e-169
Identity = 271/369 (73.44%), Postives = 312/369 (84.55%), Query Frame = 1

Query: 7   RMEKKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYF 66
           ++E ++ +RVPH+KPPFTL Q+KKAIPPHCF RS+LRSFSY++ DLT+  +F+ +A  YF
Sbjct: 15  KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFSYVVYDLTVAFIFYYIATNYF 74

Query: 67  PLLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPY 126
            LLP+PL Y+AWP YW  QGC LTGVWVIAHECGHHAFSDYQLLDDI+GL  HS LLVPY
Sbjct: 75  HLLPQPLSYVAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQLLDDIVGLVLHSCLLVPY 134

Query: 127 FSWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYL 186
           FSWK+SHRRHHSNTASL+RDEVFVPK KS I W+  Y NN PGRL +L  TLTLGWPLYL
Sbjct: 135 FSWKHSHRRHHSNTASLERDEVFVPKKKSSIRWFSKYLNNPPGRLFTLTITLTLGWPLYL 194

Query: 187 AFNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRI 246
           AFN SGR Y+RFACHYDPYGPI+T RER +IYISDAG+L + F LYR+ AAKG  WVI +
Sbjct: 195 AFNVSGRPYDRFACHYDPYGPIYTDRERTEIYISDAGVLAVTFGLYRLAAAKGLAWVICV 254

Query: 247 YGMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDT 306
           YG+PL+IVN FLV+ITYLQHTHPS+PHY+SSEWDWLRGALATVDRDYG+LN VFHNITDT
Sbjct: 255 YGVPLLIVNAFLVMITYLQHTHPSIPHYDSSEWDWLRGALATVDRDYGILNKVFHNITDT 314

Query: 307 HVVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSD 366
           HV HHLFSTMPHYHA EA++A+K +LGEYY+ D T   KA+WREAKEC+YVEAD D D  
Sbjct: 315 HVAHHLFSTMPHYHAMEASKAIKPILGEYYQFDGTPFYKAMWREAKECIYVEAD-DGDES 374

Query: 367 KGVVWYRNK 376
           KGV WY  K
Sbjct: 375 KGVYWYNKK 382

BLAST of Cla003201 vs. NCBI nr
Match: gi|332384315|gb|AEE69020.1| (Fad2 [Vernicia fordii])

HSP 1 Score: 602.4 bits (1552), Expect = 5.3e-169
Identity = 271/369 (73.44%), Postives = 311/369 (84.28%), Query Frame = 1

Query: 7   RMEKKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYF 66
           ++E ++ +RVPH+KPPFTL Q+KKAIPPHCF RS+LRSFSY++ DLT+  +F+ +A  YF
Sbjct: 15  KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFSYVVYDLTVAFIFYYIATNYF 74

Query: 67  PLLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPY 126
            LL +PL Y+AWP YW  QGC LTGVWVIAHECGHHAFSDYQLLDDI+GL  HS LLVPY
Sbjct: 75  HLLTQPLSYVAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQLLDDIVGLVLHSCLLVPY 134

Query: 127 FSWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYL 186
           FSWK+SHRRHHSNTASL+RDEVFVPK KS I W+  Y NN PGRL +L  TLTLGWPLYL
Sbjct: 135 FSWKHSHRRHHSNTASLERDEVFVPKKKSSIRWFSKYLNNPPGRLFTLTITLTLGWPLYL 194

Query: 187 AFNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRI 246
           AFN SGR Y+RFACHYDPYGPI+T RER +IYISDAG+L + F LYR+ AAKG  WVI +
Sbjct: 195 AFNVSGRPYDRFACHYDPYGPIYTDRERTEIYISDAGVLAVTFGLYRLAAAKGLAWVICV 254

Query: 247 YGMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDT 306
           YG+PL+IVN FLV+ITYLQHTHPS+PHY+SSEWDWLRGALATVDRDYG+LN VFHNITDT
Sbjct: 255 YGVPLLIVNAFLVMITYLQHTHPSIPHYDSSEWDWLRGALATVDRDYGILNKVFHNITDT 314

Query: 307 HVVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSD 366
           HV HHLFSTMPHYHA EAT+A+K +LGEYY+ D T   KA+WREAKEC+YVEAD D D  
Sbjct: 315 HVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPFYKAMWREAKECIYVEAD-DGDES 374

Query: 367 KGVVWYRNK 376
           KGV WY  K
Sbjct: 375 KGVYWYNKK 382

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
FAD12_TRIKI7.3e-20388.35Delta(12)-oleate desaturase OS=Trichosanthes kirilowii GN=FAD2 PE=2 SV=1[more]
FAD2_VERFO3.9e-17273.71Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii GN=FAD2 PE=1 SV=1[more]
FAD12_PUNGR2.4e-16670.13Delta(12)-acyl-lipid-desaturase OS=Punica granatum GN=FAD12 PE=2 SV=2[more]
FD6E2_SOYBN5.6e-16372.18Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max GN... [more]
FH12_RICCO7.6e-16069.61Phosphatidylcholine 12-monooxygenase OS=Ricinus communis GN=FAH12 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
W9QMT8_9ROSA1.1e-17075.76Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Morus notabili... [more]
I6LVA4_VERFO1.3e-16973.44Fad2 OS=Vernicia fordii GN=fad2 PE=4 SV=1[more]
I6LVA3_VERFO3.7e-16973.44Fad2 OS=Vernicia fordii GN=fad2 PE=4 SV=1[more]
A0A068VA14_COFCA4.8e-16974.66Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00011472001 PE=4 SV=1[more]
U5TMI2_MILPI6.9e-16874.31Microsomal omega-6-desaturase OS=Millettia pinnata GN=FAD2-2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
gi|75328134|sp|Q84UB9.1|FAD12_TRIKI1.2e-20088.35RecName: Full=Delta(12)-oleate desaturase; Short=PgFAD2[more]
gi|703065220|ref|XP_010087393.1|1.6e-17075.76Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 [Morus notabilis][more]
gi|75329014|sp|Q8GZC3.1|FAD2_VERFO6.2e-17073.71RecName: Full=Delta(12)-fatty-acid desaturase FAD2[more]
gi|332384317|gb|AEE69021.1|1.8e-16973.44Fad2 [Vernicia fordii][more]
gi|332384315|gb|AEE69020.1|5.3e-16973.44Fad2 [Vernicia fordii][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005804FA_desaturase_dom
IPR021863FAS_N
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016717oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006636 unsaturated fatty acid biosynthetic process
cellular_component GO:0005575 cellular_component
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016020 membrane
molecular_function GO:0016720 delta12-fatty acid dehydrogenase activity
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
WMU42504watermelon EST collection version 2.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla003201Cla003201.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
WMU42504WMU42504transcribed_cluster


Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 77..337
score: 6.4
IPR021863Fatty acid desaturase, N-terminalPFAMPF11960DUF3474coord: 10..56
score: 2.
NoneNo IPR availablePANTHERPTHR32100FAMILY NOT NAMEDcoord: 6..376
score: 1.1E
NoneNo IPR availablePANTHERPTHR32100:SF13OMEGA-6 FATTY ACID DESATURASE, ENDOPLASMIC RETICULUMcoord: 6..376
score: 1.1E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cla003201Cla017539Watermelon (97103) v1wmwmB051