CSPI04G13350 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI04G13350
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionFatty acid desaturase
LocationChr4 : 11412197 .. 11412823 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCCTCACACTTGGCTGGCCAATGTACATAGCCTTCAACAATTCAGGCCGAGTCTATGATAGATTCACCAGCCACTACGATCCAAACAGCCCAATATTCACCGAAAAGGAACGACTTCAAGTTCAAATATCCAATGCCGGAGTCTTAACCATTTTATACTTACTTTACAAACTCGCAGTTACAAAAGGAATCACGTGGGTCATTCGCATATACTTACTGCCTTTAACGGTTATGAACGTGTTTGTGGTGTTGATATCATGTTTACAACACACACACTCTTCATTGCCGTATTACGATTCTAGTCAGTGGGATTGGCTGAGAGGGAATTTGGTTACAGTGGATAGAGATTACGGGAAGATTTTGAATAAAATGTTACATAATATAACGGATACACATGTGATTCATCACTTGTTCCCTTCAATGCCACATTACAATGCAGCGGAGGCGACGAGGGCGGTGAAGGAAGTGCTGGGAGAGTACTATCAGTTTGATGAGACGCCTATCTTGAAGGCTGCATGGAGGGAGTTTAGAGAATGTGTTTATGTAGAGGAAGATGATCATGAGGAGGGGGACTCAGAAGCTTTTAGAAGTAAAGGGGTATTTTGGTTTCGGAACAAGCTTTGA

mRNA sequence

ATGACCCTCACACTTGGCTGGCCAATGTACATAGCCTTCAACAATTCAGGCCGAGTCTATGATAGATTCACCAGCCACTACGATCCAAACAGCCCAATATTCACCGAAAAGGAACGACTTCAAGTTCAAATATCCAATGCCGGAGTCTTAACCATTTTATACTTACTTTACAAACTCGCAGTTACAAAAGGAATCACGTGGGTCATTCGCATATACTTACTGCCTTTAACGGTTATGAACGTGTTTGTGGTGTTGATATCATGTTTACAACACACACACTCTTCATTGCCGTATTACGATTCTAGTCAGTGGGATTGGCTGAGAGGGAATTTGGTTACAGTGGATAGAGATTACGGGAAGATTTTGAATAAAATGTTACATAATATAACGGATACACATGTGATTCATCACTTGTTCCCTTCAATGCCACATTACAATGCAGCGGAGGCGACGAGGGCGGTGAAGGAAGTGCTGGGAGAGTACTATCAGTTTGATGAGACGCCTATCTTGAAGGCTGCATGGAGGGAGTTTAGAGAATGTGTTTATGTAGAGGAAGATGATCATGAGGAGGGGGACTCAGAAGCTTTTAGAAGTAAAGGGGTATTTTGGTTTCGGAACAAGCTTTGA

Coding sequence (CDS)

ATGACCCTCACACTTGGCTGGCCAATGTACATAGCCTTCAACAATTCAGGCCGAGTCTATGATAGATTCACCAGCCACTACGATCCAAACAGCCCAATATTCACCGAAAAGGAACGACTTCAAGTTCAAATATCCAATGCCGGAGTCTTAACCATTTTATACTTACTTTACAAACTCGCAGTTACAAAAGGAATCACGTGGGTCATTCGCATATACTTACTGCCTTTAACGGTTATGAACGTGTTTGTGGTGTTGATATCATGTTTACAACACACACACTCTTCATTGCCGTATTACGATTCTAGTCAGTGGGATTGGCTGAGAGGGAATTTGGTTACAGTGGATAGAGATTACGGGAAGATTTTGAATAAAATGTTACATAATATAACGGATACACATGTGATTCATCACTTGTTCCCTTCAATGCCACATTACAATGCAGCGGAGGCGACGAGGGCGGTGAAGGAAGTGCTGGGAGAGTACTATCAGTTTGATGAGACGCCTATCTTGAAGGCTGCATGGAGGGAGTTTAGAGAATGTGTTTATGTAGAGGAAGATGATCATGAGGAGGGGGACTCAGAAGCTTTTAGAAGTAAAGGGGTATTTTGGTTTCGGAACAAGCTTTGA
Homology
[Close]
BLAST of CSPI04G13350 vs. Swiss-Prot
Match: FADX_TRIKI (Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase OS=Trichosanthes kirilowii GN=FAC PE=2 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 2.7e-90
Identity = 156/208 (75.00%), Postives = 179/208 (86.06%), Query Frame = 1 

Query: 1   MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA 60
           +TLTLGWPMY+AFN SGR Y+RFTSH+DPNSPIF+E E LQV ISNAG++ + YLLYKLA
Sbjct: 184 ITLTLGWPMYLAFNISGRFYERFTSHFDPNSPIFSENEWLQVHISNAGIVAVWYLLYKLA 243

Query: 61  VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK 120
             KGI WVIR+Y++P+T+MN FVVLI+ LQHTH S PYYDS++W+WLRGNLVT+DRDYG 
Sbjct: 244 AAKGIAWVIRMYVVPVTIMNAFVVLITSLQHTHPSFPYYDSTEWNWLRGNLVTLDRDYG- 303

Query: 121 ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC 180
           ILNK+ HNITDTHV+HHLFPSMPHYNA EATRAVK+VLGEYY FD TPI KAAWREFREC
Sbjct: 304 ILNKVFHNITDTHVVHHLFPSMPHYNAMEATRAVKQVLGEYYHFDGTPIFKAAWREFREC 363

Query: 181 VYVEEDDHEEGDSEAFRSKGVFWFRNKL 209
           +YVE D+ E   S    SKGVFWFRNKL
Sbjct: 364 IYVEPDNDEGASSS---SKGVFWFRNKL 387

[Close]
BLAST of CSPI04G13350 vs. Swiss-Prot
Match: FADX_MOMCH (Delta(12) acyl-lipid conjugase (11E,13E-forming) OS=Momordica charantia GN=FADX PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 4.9e-84
Identity = 143/208 (68.75%), Postives = 172/208 (82.69%), Query Frame = 1 

Query: 1   MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA 60
           +TLTLGWPMY+ FN SGR Y RFTSH+DPNSPIF+ KER+ V ISNAG++   YLLY++A
Sbjct: 193 ITLTLGWPMYLTFNISGRYYGRFTSHFDPNSPIFSPKERVLVHISNAGLVATGYLLYRIA 252

Query: 61  VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK 120
           + KG+ W+IR+Y +PL V+N  VVLI+ LQHTH S PYYDS++WDWLRGNLVTVDRDYG 
Sbjct: 253 MAKGVGWLIRLYGVPLIVLNACVVLITALQHTHPSFPYYDSTEWDWLRGNLVTVDRDYGP 312

Query: 121 ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC 180
           I+N++ H+ITDTHV+HHLFPSMPHYN  EAT A K +LGEYYQFD TPI KAAWREFREC
Sbjct: 313 IMNRVFHHITDTHVVHHLFPSMPHYNGKEATVAAKRILGEYYQFDGTPIWKAAWREFREC 372

Query: 181 VYVEEDDHEEGDSEAFRSKGVFWFRNKL 209
           VYVE D+ ++G +    SKGVFW+ NKL
Sbjct: 373 VYVEPDE-DDGATSGSSSKGVFWYHNKL 399

[Close]
BLAST of CSPI04G13350 vs. Swiss-Prot
Match: FD6E2_SOYBN (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max GN=FAD2-2 PE=2 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 8.5e-76
Identity = 132/208 (63.46%), Postives = 166/208 (79.81%), Query Frame = 1 

Query: 1   MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA 60
           +TLTLGWP+Y+A N SGR YDRF  HYDP  PI++++ERLQ+ IS+AGVL ++Y L++LA
Sbjct: 184 VTLTLGWPLYLALNVSGRPYDRFACHYDPYGPIYSDRERLQIYISDAGVLAVVYGLFRLA 243

Query: 61  VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK 120
           + KG+ WV+ +Y +PL V+N F+VLI+ LQHTH +LP+Y SS+WDWLRG L TVDRDYG 
Sbjct: 244 MAKGLAWVVCVYGVPLLVVNGFLVLITFLQHTHPALPHYTSSEWDWLRGALATVDRDYG- 303

Query: 121 ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC 180
           ILNK+ HNITDTHV HHLF +MPHY+A EAT+A+K +LGEYY+FDETP +KA WRE REC
Sbjct: 304 ILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRFDETPFVKAMWREAREC 363

Query: 181 VYVEEDDHEEGDSEAFRSKGVFWFRNKL 209
           +YVE D   E       SKGVFW+ NKL
Sbjct: 364 IYVEPDQSTE-------SKGVFWYNNKL 383

[Close]
BLAST of CSPI04G13350 vs. Swiss-Prot
Match: FAD2_VERFO (Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii GN=FAD2 PE=1 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 4.2e-75
Identity = 129/207 (62.32%), Postives = 163/207 (78.74%), Query Frame = 1 

Query: 1   MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA 60
           +TLTLGWP+Y+AFN SGR YDRF  HYDP  PI+T++ER ++ IS+AGVL + + LY+LA
Sbjct: 184 ITLTLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERTEIYISDAGVLAVTFGLYRLA 243

Query: 61  VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK 120
             KG+ WVI +Y +PL ++N F+V+I+ LQHTH S+P+YDSS+WDWLRG L TVDRDYG 
Sbjct: 244 AAKGLAWVICVYGVPLLIVNAFLVMITYLQHTHPSIPHYDSSEWDWLRGALATVDRDYG- 303

Query: 121 ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC 180
           ILNK+ HNITDTHV HHLF +MPHY+A EAT+A+K +LGEYYQFD TP  KA WRE +EC
Sbjct: 304 ILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPFYKAMWREAKEC 363

Query: 181 VYVEEDDHEEGDSEAFRSKGVFWFRNK 208
           +YVE DD +E       SKGV+W+  K
Sbjct: 364 IYVEADDGDE-------SKGVYWYNKK 382

[Close]
BLAST of CSPI04G13350 vs. Swiss-Prot
Match: FAD12_TRIKI (Delta(12)-oleate desaturase OS=Trichosanthes kirilowii GN=FAD2 PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 4.2e-75
Identity = 133/207 (64.25%), Postives = 167/207 (80.68%), Query Frame = 1 

Query: 2   TLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLAV 61
           TLTLGWP+Y+AFN SGR YDRF  HYDP SPIFT++E +QV IS+AG+L + ++LY++ V
Sbjct: 170 TLTLGWPLYLAFNMSGRPYDRFACHYDPYSPIFTKREWIQVYISDAGILGMAFMLYRIGV 229

Query: 62  TKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGKI 121
            KG  WV+RIY +PL ++N F+VLI+ LQHTH +LP+Y+SS+WDWLRG L TVDRDYG +
Sbjct: 230 EKGSFWVMRIYGIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYG-V 289

Query: 122 LNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFRECV 181
           LN++ HNITDTHV+HHLF +MPHY+A EAT AVK VLGEYY+ D TP+LKA WRE +ECV
Sbjct: 290 LNRVFHNITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDRTPVLKAIWREAKECV 349

Query: 182 YVEEDDHEEGDSEAFRSKGVFWFRNKL 209
           YVE D  E+ DS+    KGV W+R KL
Sbjct: 350 YVEPD--EDADSD----KGVVWYRKKL 369

[Close]
BLAST of CSPI04G13350 vs. TrEMBL
Match: A0A0A0KWW8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G286350 PE=4 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 3.7e-118
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 1 

Query: 1   MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA 60
           MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA
Sbjct: 1   MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA 60

Query: 61  VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK 120
           VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK
Sbjct: 61  VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK 120

Query: 121 ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC 180
           ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC
Sbjct: 121 ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC 180

Query: 181 VYVEEDDHEEGDSEAFRSKGVFWFRNKL 209
           VYVEEDDHEEGDSEAFRSKGVFWFRNKL
Sbjct: 181 VYVEEDDHEEGDSEAFRSKGVFWFRNKL 208

[Close]
BLAST of CSPI04G13350 vs. TrEMBL
Match: A0A0V0I7N0_SOLCH (Putative omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2-like OS=Solanum chacoense PE=4 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 5.0e-75
Identity = 132/208 (63.46%), Postives = 168/208 (80.77%), Query Frame = 1 

Query: 1   MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA 60
           +TLTLGWP+Y+AFN SGR YDRF  HYDP  PI+  +ERLQ+ +S+AGVL + YLLY++A
Sbjct: 119 ITLTLGWPLYLAFNVSGRPYDRFACHYDPYGPIYNNRERLQIFVSDAGVLGVCYLLYRIA 178

Query: 61  VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK 120
           + KG+ W++ IY +PL V+N F+VLI+ LQHTH SLP+YDS++WDWLRG L T DRDYG 
Sbjct: 179 LVKGLAWLVCIYGVPLLVVNGFLVLITYLQHTHPSLPHYDSTEWDWLRGALATCDRDYG- 238

Query: 121 ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC 180
           +LNK+ HNITDTHV+HHLF +MPHYNA EAT+AVK +LG+YYQFD TPI KA WRE +EC
Sbjct: 239 VLNKVFHNITDTHVVHHLFSTMPHYNAMEATKAVKPLLGDYYQFDGTPIFKAMWREAKEC 298

Query: 181 VYVEEDDHEEGDSEAFRSKGVFWFRNKL 209
           +YVE+D+  +G       KGVFW++NKL
Sbjct: 299 LYVEKDESSQG-------KGVFWYKNKL 318

[Close]
BLAST of CSPI04G13350 vs. TrEMBL
Match: M1D5H1_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400032204 PE=4 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 6.5e-75
Identity = 132/208 (63.46%), Postives = 168/208 (80.77%), Query Frame = 1 

Query: 1   MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA 60
           +TLTLGWP+Y+AFN SGR YDRF  HYDP  PI+  +ERLQ+ +S+AGVL + YLLY++A
Sbjct: 184 ITLTLGWPLYLAFNVSGRPYDRFACHYDPYGPIYNNRERLQIFVSDAGVLGVCYLLYRVA 243

Query: 61  VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK 120
           + KG+ W++ IY +PL V+N F+VLI+ LQHTH SLP+YDS++WDWLRG L T DRDYG 
Sbjct: 244 LVKGLAWLVCIYGVPLLVVNGFLVLITYLQHTHPSLPHYDSTEWDWLRGALATCDRDYG- 303

Query: 121 ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC 180
           +LNK+ HNITDTHV+HHLF +MPHYNA EAT+AVK +LG+YYQFD TPI KA WRE +EC
Sbjct: 304 VLNKVFHNITDTHVVHHLFSTMPHYNAMEATKAVKPLLGDYYQFDGTPIFKAMWREAKEC 363

Query: 181 VYVEEDDHEEGDSEAFRSKGVFWFRNKL 209
           +YVE+D+  +G       KGVFW++NKL
Sbjct: 364 LYVEKDESSQG-------KGVFWYKNKL 383

[Close]
BLAST of CSPI04G13350 vs. TrEMBL
Match: Q6DMQ8_TOBAC (Microsomal omega-6-desaturase OS=Nicotiana tabacum GN=FAD2 PE=2 SV=2)

HSP 1 Score: 288.5 bits (737), Expect = 6.5e-75
Identity = 133/208 (63.94%), Postives = 169/208 (81.25%), Query Frame = 1 

Query: 1   MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA 60
           +TLTLGWP+Y+AFN SGR YDRF  HYDP  PI+ ++ERLQ+ +S+AGVL   YLLY++A
Sbjct: 184 VTLTLGWPLYLAFNVSGRHYDRFACHYDPYGPIYNDRERLQIFLSDAGVLGAGYLLYRIA 243

Query: 61  VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK 120
           + KG+ W++ +Y +PL ++N F+VLI+ LQHTH SLP+YDSS+WDWLRG L TVDRDYG 
Sbjct: 244 LVKGLAWLVCMYGVPLLIVNGFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRDYG- 303

Query: 121 ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC 180
           ILNK+ HNITDTHV+HHLF +MPHYNA EAT+AVK +LG+YYQFD TP+ KA WRE +EC
Sbjct: 304 ILNKVFHNITDTHVVHHLFSTMPHYNAMEATKAVKPLLGDYYQFDGTPVFKAMWREAKEC 363

Query: 181 VYVEEDDHEEGDSEAFRSKGVFWFRNKL 209
           +YVE+D       EA + KGVFW++NKL
Sbjct: 364 IYVEKD-------EASQGKGVFWYKNKL 383

[Close]
BLAST of CSPI04G13350 vs. TrEMBL
Match: Q461Q1_HEVBR (Omega-6 fatty acid desaturase OS=Hevea brasiliensis GN=FAD PE=2 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.1e-74
Identity = 133/207 (64.25%), Postives = 165/207 (79.71%), Query Frame = 1 

Query: 1   MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA 60
           +TLTLGWP+Y+AFN SGR Y+RF  HYDP  PI+T++ER ++ IS+AGVL + Y LY LA
Sbjct: 184 ITLTLGWPLYLAFNVSGRPYNRFACHYDPYGPIYTDRERTEICISDAGVLAVTYGLYSLA 243

Query: 61  VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK 120
           V KG+ WV+ +Y +PL V+N F+V+I+ LQHTH SLP+YDSS+WDWLRG L TVDRDYG 
Sbjct: 244 VAKGLAWVVCVYGVPLLVVNAFLVMITYLQHTHPSLPHYDSSEWDWLRGALATVDRDYG- 303

Query: 121 ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC 180
           ILNK+ HNITDTHV HHLF +MPHYNA EAT+A+K +LGEYYQFD TP  KA WRE +EC
Sbjct: 304 ILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGEYYQFDGTPFYKAMWREAKEC 363

Query: 181 VYVEEDDHEEGDSEAFRSKGVFWFRNK 208
           VYVE DD ++       SKGV+W++NK
Sbjct: 364 VYVEPDDRDQ-------SKGVYWYKNK 382

[Close]
BLAST of CSPI04G13350 vs. TAIR10
Match: AT3G12120.1 (AT3G12120.1 fatty acid desaturase 2)

HSP 1 Score: 257.3 bits (656), Expect = 8.1e-69
Identity = 121/204 (59.31%), Postives = 157/204 (76.96%), Query Frame = 1 

Query: 5   LGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLAVTKG 64
           LGWP+Y+AFN SGR YD F  H+ PN+PI+ ++ERLQ+ +S+AG+L + + LY+ A  +G
Sbjct: 188 LGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLYRYAAAQG 247

Query: 65  ITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGKILNK 124
           +  +I +Y +PL ++N F+VLI+ LQHTH SLP+YDSS+WDWLRG L TVDRDYG ILNK
Sbjct: 248 MASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRDYG-ILNK 307

Query: 125 MLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFRECVYVE 184
           + HNITDTHV HHLF +MPHYNA EAT+A+K +LG+YYQFD TP   A +RE +EC+YVE
Sbjct: 308 VFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAKECIYVE 367

Query: 185 EDDHEEGDSEAFRSKGVFWFRNKL 209
            D   EGD      KGV+W+ NKL
Sbjct: 368 PD--REGD-----KKGVYWYNNKL 383

[Close]
BLAST of CSPI04G13350 vs. TAIR10
Match: AT3G11170.1 (AT3G11170.1 fatty acid desaturase 7)

HSP 1 Score: 110.9 bits (276), Expect = 9.4e-25
Identity = 64/161 (39.75%), Postives = 92/161 (57.14%), Query Frame = 1 

Query: 5   LGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLAVTKG 64
           L +P Y+   + G+      SHY P+S +F  KER  V  S A    +  LL  L  T G
Sbjct: 242 LAYPFYLWARSPGKK----GSHYHPDSDLFLPKERKDVLTSTACWTAMAALLVCLNFTIG 301

Query: 65  ITWVIRIYLLPLTVMNVFVVLISCLQHT--HSSLPYYDSSQWDWLRGNLVTVDRDYGKIL 124
              ++++Y +P  +  +++  ++ L H      LP+Y   +W +LRG L T+DRDYG I 
Sbjct: 302 PIQMLKLYGIPYWINVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGLIN 361

Query: 125 NKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQ 164
           N  +H+   THVIHHLFP +PHY+  EAT A K VLG+YY+
Sbjct: 362 N--IHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVLGKYYR 396

[Close]
BLAST of CSPI04G13350 vs. TAIR10
Match: AT5G05580.1 (AT5G05580.1 fatty acid desaturase 8)

HSP 1 Score: 108.6 bits (270), Expect = 4.7e-24
Identity = 62/161 (38.51%), Postives = 91/161 (56.52%), Query Frame = 1 

Query: 5   LGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLAVTKG 64
           L +P Y+   + G+      SHY P+S +F  KE+  V  S A    +  LL  L    G
Sbjct: 235 LAYPFYLWNRSPGKQ----GSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVCLNFVMG 294

Query: 65  ITWVIRIYLLPLTVMNVFVVLISCLQHT--HSSLPYYDSSQWDWLRGNLVTVDRDYGKIL 124
              ++++Y +P  +  +++  ++ L H      LP+Y   +W +LRG L T+DRDYG I 
Sbjct: 295 PIQMLKLYGIPYWIFVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGWIN 354

Query: 125 NKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQ 164
           N  +H+   THVIHHLFP +PHY+  EAT A K VLG+YY+
Sbjct: 355 N--IHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVLGKYYR 389

[Close]
BLAST of CSPI04G13350 vs. TAIR10
Match: AT2G29980.1 (AT2G29980.1 fatty acid desaturase 3)

HSP 1 Score: 108.2 bits (269), Expect = 6.1e-24
Identity = 60/165 (36.36%), Postives = 94/165 (56.97%), Query Frame = 1 

Query: 5   LGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLAVTKG 64
           L +P+Y+ + + G+      SH++P S +F   ER  +  S      +   L  L+   G
Sbjct: 180 LAYPLYLCYRSPGKE----GSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFG 239

Query: 65  ITWVIRIYLLPLTVMNVFVVLISCLQHT--HSSLPYYDSSQWDWLRGNLVTVDRDYGKIL 124
              V+++Y +P  +  +++  ++ L H      LP+Y   +W +LRG L T+DRDYG I 
Sbjct: 240 PLAVLKVYGVPYIIFVMWLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYG-IF 299

Query: 125 NKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDET 168
           N + H+I  THVIHHLFP +PHY+  +AT+A K VLG YY+  +T
Sbjct: 300 NNIHHDI-GTHVIHHLFPQIPHYHLVDATKAAKHVLGRYYREPKT 338

[Close]
BLAST of CSPI04G13350 vs. NCBI nr
Match: gi|778693060|ref|XP_011653570.1| (PREDICTED: omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2-like [Cucumis sativus])

HSP 1 Score: 432.2 bits (1110), Expect = 5.2e-118
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 1 

Query: 1   MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA 60
           MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA
Sbjct: 180 MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA 239

Query: 61  VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK 120
           VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK
Sbjct: 240 VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK 299

Query: 121 ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC 180
           ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC
Sbjct: 300 ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC 359

Query: 181 VYVEEDDHEEGDSEAFRSKGVFWFRNKL 209
           VYVEEDDHEEGDSEAFRSKGVFWFRNKL
Sbjct: 360 VYVEEDDHEEGDSEAFRSKGVFWFRNKL 387

[Close]
BLAST of CSPI04G13350 vs. NCBI nr
Match: gi|700198952|gb|KGN54110.1| (hypothetical protein Csa_4G286350 [Cucumis sativus])

HSP 1 Score: 432.2 bits (1110), Expect = 5.2e-118
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 1 

Query: 1   MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA 60
           MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA
Sbjct: 1   MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA 60

Query: 61  VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK 120
           VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK
Sbjct: 61  VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK 120

Query: 121 ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC 180
           ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC
Sbjct: 121 ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC 180

Query: 181 VYVEEDDHEEGDSEAFRSKGVFWFRNKL 209
           VYVEEDDHEEGDSEAFRSKGVFWFRNKL
Sbjct: 181 VYVEEDDHEEGDSEAFRSKGVFWFRNKL 208

[Close]
BLAST of CSPI04G13350 vs. NCBI nr
Match: gi|659098044|ref|XP_008449949.1| (PREDICTED: omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2-like [Cucumis melo])

HSP 1 Score: 420.6 bits (1080), Expect = 1.6e-114
Identity = 202/208 (97.12%), Postives = 205/208 (98.56%), Query Frame = 1 

Query: 1   MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA 60
           MTLTLGWPMY+AFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA
Sbjct: 179 MTLTLGWPMYLAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA 238

Query: 61  VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK 120
           VTKGITWVIR+YLLPLTVMNVFVVLIS LQHTH SLPYYDSSQWDWLRGNLVTVDRDYGK
Sbjct: 239 VTKGITWVIRLYLLPLTVMNVFVVLISSLQHTHPSLPYYDSSQWDWLRGNLVTVDRDYGK 298

Query: 121 ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC 180
           ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC
Sbjct: 299 ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC 358

Query: 181 VYVEEDDHEEGDSEAFRSKGVFWFRNKL 209
           VYVEEDD+EEGDSE FRSKGVFWFRNKL
Sbjct: 359 VYVEEDDNEEGDSEGFRSKGVFWFRNKL 386

[Close]
BLAST of CSPI04G13350 vs. NCBI nr
Match: gi|75328135|sp|Q84UC0.1|FADX_TRIKI (RecName: Full=Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase; Short=TkFAC; AltName: Full=Delta(12)-oleate desaturase; AltName: Full=Linoleoyl-lipid Delta(12) conjugase (11E,13Z-forming))

HSP 1 Score: 332.8 bits (852), Expect = 4.3e-88
Identity = 156/208 (75.00%), Postives = 179/208 (86.06%), Query Frame = 1 

Query: 1   MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA 60
           +TLTLGWPMY+AFN SGR Y+RFTSH+DPNSPIF+E E LQV ISNAG++ + YLLYKLA
Sbjct: 184 ITLTLGWPMYLAFNISGRFYERFTSHFDPNSPIFSENEWLQVHISNAGIVAVWYLLYKLA 243

Query: 61  VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK 120
             KGI WVIR+Y++P+T+MN FVVLI+ LQHTH S PYYDS++W+WLRGNLVT+DRDYG 
Sbjct: 244 AAKGIAWVIRMYVVPVTIMNAFVVLITSLQHTHPSFPYYDSTEWNWLRGNLVTLDRDYG- 303

Query: 121 ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC 180
           ILNK+ HNITDTHV+HHLFPSMPHYNA EATRAVK+VLGEYY FD TPI KAAWREFREC
Sbjct: 304 ILNKVFHNITDTHVVHHLFPSMPHYNAMEATRAVKQVLGEYYHFDGTPIFKAAWREFREC 363

Query: 181 VYVEEDDHEEGDSEAFRSKGVFWFRNKL 209
           +YVE D+ E   S    SKGVFWFRNKL
Sbjct: 364 IYVEPDNDEGASSS---SKGVFWFRNKL 387

[Close]
BLAST of CSPI04G13350 vs. NCBI nr
Match: gi|75337482|sp|Q9SP61.1|FADX_MOMCH (RecName: Full=Delta(12) acyl-lipid conjugase (11E,13E-forming); Short=MomoFadX)

HSP 1 Score: 312.0 bits (798), Expect = 7.9e-82
Identity = 143/208 (68.75%), Postives = 172/208 (82.69%), Query Frame = 1 

Query: 1   MTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLA 60
           +TLTLGWPMY+ FN SGR Y RFTSH+DPNSPIF+ KER+ V ISNAG++   YLLY++A
Sbjct: 193 ITLTLGWPMYLTFNISGRYYGRFTSHFDPNSPIFSPKERVLVHISNAGLVATGYLLYRIA 252

Query: 61  VTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGK 120
           + KG+ W+IR+Y +PL V+N  VVLI+ LQHTH S PYYDS++WDWLRGNLVTVDRDYG 
Sbjct: 253 MAKGVGWLIRLYGVPLIVLNACVVLITALQHTHPSFPYYDSTEWDWLRGNLVTVDRDYGP 312

Query: 121 ILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFREC 180
           I+N++ H+ITDTHV+HHLFPSMPHYN  EAT A K +LGEYYQFD TPI KAAWREFREC
Sbjct: 313 IMNRVFHHITDTHVVHHLFPSMPHYNGKEATVAAKRILGEYYQFDGTPIWKAAWREFREC 372

Query: 181 VYVEEDDHEEGDSEAFRSKGVFWFRNKL 209
           VYVE D+ ++G +    SKGVFW+ NKL
Sbjct: 373 VYVEPDE-DDGATSGSSSKGVFWYHNKL 399

The following BLAST results are available for this feature:
BLAST of CSPI04G13350 vs. Swiss-Prot
Match NameE-valueIdentityDescription
FADX_TRIKI2.7e-9075.00Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase OS=Trichosanthes k... [more]
FADX_MOMCH4.9e-8468.75Delta(12) acyl-lipid conjugase (11E,13E-forming) OS=Momordica charantia GN=FADX ... [more]
FD6E2_SOYBN8.5e-7663.46Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max GN... [more]
FAD2_VERFO4.2e-7562.32Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii GN=FAD2 PE=1 SV=1[more]
FAD12_TRIKI4.2e-7564.25Delta(12)-oleate desaturase OS=Trichosanthes kirilowii GN=FAD2 PE=2 SV=1[more]
BLAST of CSPI04G13350 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KWW8_CUCSA3.7e-118100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G286350 PE=4 SV=1[more]
A0A0V0I7N0_SOLCH5.0e-7563.46Putative omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2-like OS=... [more]
M1D5H1_SOLTU6.5e-7563.46Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400032204 PE=4 SV=1[more]
Q6DMQ8_TOBAC6.5e-7563.94Microsomal omega-6-desaturase OS=Nicotiana tabacum GN=FAD2 PE=2 SV=2[more]
Q461Q1_HEVBR1.1e-7464.25Omega-6 fatty acid desaturase OS=Hevea brasiliensis GN=FAD PE=2 SV=1[more]
BLAST of CSPI04G13350 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G12120.18.1e-6959.31 fatty acid desaturase 2[more]
AT3G11170.19.4e-2539.75 fatty acid desaturase 7[more]
AT5G05580.14.7e-2438.51 fatty acid desaturase 8[more]
AT2G29980.16.1e-2436.36 fatty acid desaturase 3[more]
BLAST of CSPI04G13350 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778693060|ref|XP_011653570.1|5.2e-118100.00PREDICTED: omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2-like [... [more]
gi|700198952|gb|KGN54110.1|5.2e-118100.00hypothetical protein Csa_4G286350 [Cucumis sativus][more]
gi|659098044|ref|XP_008449949.1|1.6e-11497.12PREDICTED: omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2-like [... [more]
gi|75328135|sp|Q84UC0.1|FADX_TRIKI4.3e-8875.00RecName: Full=Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase; Sho... [more]
gi|75337482|sp|Q9SP61.1|FADX_MOMCH7.9e-8268.75RecName: Full=Delta(12) acyl-lipid conjugase (11E,13E-forming); Short=MomoFadX[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005804FA_desaturase_dom
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006636 unsaturated fatty acid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0016720 delta12-fatty acid dehydrogenase activity
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G13350.1CSPI04G13350.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 32..163
score: 7.4
NoneNo IPR availablePANTHERPTHR32100FAMILY NOT NAMEDcoord: 1..208
score: 1.0E
NoneNo IPR availablePANTHERPTHR32100:SF13OMEGA-6 FATTY ACID DESATURASE, ENDOPLASMIC RETICULUMcoord: 1..208
score: 1.0E