ClCG07G012080 (gene) Watermelon (Charleston Gray)

NameClCG07G012080
Typegene
OrganismCitrullus lanatus (Watermelon (Charleston Gray))
Description3-beta-hydroxysteroid-delta-isomerase
LocationCG_Chr07 : 28441417 .. 28444418 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCACAGGATGAAGCTCGAAGTGAAAGAACAGCGAGTTCATTGGTCAAACTTGAAACCAGAGGGAAAAAGAAGTCATCAATGGCGGGGGAATCTGAAGCTGCCCCGCATCCCTACGTTCCCACAGATCTGAAACTCCCCGAATATGTTCCTATTTCACTCCCTTTCTCTACGATTCTCGGCGTCTATCTCTTCTCTTCTCTTTTCGTCACTCTCCTCGTCTGGACCCTTTCCGGTATGCTTTCATCTTTTACTTCGAGTTTTACTTTCGATTCTGTTCTGTGTTCTTCAAATTCTGCTTCATGCTGTGTTGGAGCCGAACACCGATGGTTTTCATAATCTCTAGTTTGTTATTGCGTCGGATCCCTGTCTACTTGTCAGAAGTCACATGTTGAGTGAATTAATCGGGAGCAAAATCAAACAATTTCAAACATTCTACTCTTTTTCGTCTCCTCTGAGATTTTTTTTGCATTTCGCTTTTTCAATGTCTCAGGCTGGTGATGAAACCGATGTGAAAATTCAAGTCTGTGTCTCGTTCAATTATTCTGTTGTTTTTCTTTTCGAGAGGATCAAGATCCGCTAAACAAATAGAGACGCTAGATTTCACTTGCCTTGCTACTCCTCTGACTTGTGCTGTTGTCTTTTTGGTTTAGATGATTGCTTTAGAATGGCAATATTGATGGTACGGACAAAGCTAGGATGTCTTTTGGTCTATATGTCCAAGATTTGGTCCCTGTTAAATTAGTACCAGAACATTTTTGATTGGAAATGGATCTTTTAAGTCTAGGAGAATATTGATGGAGATGTTGTATGTCAGAGAAGTTGTTCAATAGCTTAGCATTCATGACGGATGGATCATTTCAGTTGCATTTTGGAAAAATAAGGAGATTCAAAGGATGATGGAAGGAGAATGCAGAAGAAGCCGGACGTGAACTAAACGAAGGGGGAAGAAACAAAATTCATGGTGAAGGTAGACTGTTGTACAAATATTGGATCCTAACACCATGAATGAAGTAGGAAACACTTGATAATTGGCAATATATTCAAAGATTGTATCAAGGGTTCGAAGTAGGGAGAGTCACAATTGCCAACAACAGAAGCAAAACAACTTTTTTTATTTTATTTTATTTTATTTTTTTTATAACTGTGAGTTGTCAGGCCAGTTTATGTGCACCTCGACCAATGTCACGGATAACCTGCTTGACTCTACAGCACTTGGGTGTCAAGGAAACCCATAGGATATTAAATTCTAGGTAAGTGGCCACTATAAATTGAATCTATGACTTTTTAGCTCTTTATCAAGGTCATGCCCCCTATTTTCACTATGTCAATCCATGATGGTTAGCGACCAATCATGTTGGTGTAAATTCATTGGGGAAGTAACAAAGGGAGGTAGATTCTAGAGAGTAGTCAAGAGGGTTTGGGTCCTTGTAATTTATTGCTAGTATCAATACCATGTTCTCTGTTACTTTTCCTTCAATCTTTGGTTTGTATCTGCTCTTGGAAATGGATTTTTAATATGGGAGAATGTTGTTGGAAACTTGTGATGTAGAAAAGAAATTGTTGAACGATTTAGCATCCATGAGGGAGAAGAAAATTGAGGACCATCTCAGTAACTTTTCAGAAGAATCATGACGTTCATTGTGATTGCAATACTAGGATTGGAAAAAAAAAATGTTTGACGTTGGGCAGGAAAGCTCAGGTCTAGACATTTTGCTTTGTTTTGTTCTTATAAGAAACCATAGAAGCAGTCACCAATCTTTGTGAACTTAAAAACTCATGTTGGTGTCACATCCTTCCTTGTTGCAACCTTCTCAGTTCAGATTTACAACATAAATGTCATTTTAGCATATAGTGGAAGAAATCGATCTCCAGTCCTAGTGTCTATACTTGTTTTCTATATCTTTATCTAATTCTTTTTTATCTGACTTTAGCAATGGAAAAAGGTGTGGTTCTTACAGCCGTTTAGAAATGGTTCTATGCTTGTTGATTTTATCTTCATTTTCCCCTTATAACAGGACGACTTACCAAAAAGACAAAGATTGAGAGGTTGCTCATGTGCTGGTGGATATTTACTGGCCTTACCCACATGGTTCTCGAGGGTTACTTTGTTTTCTCTCCAGAATTTTACAAGGACAAGACTTCTTTCTACCTTGCTGAAGTTTGTAAGTTAATTTGATTCACTATCTCTACAACCACAGCTTGTGGTAATAAAAGGGCTGTATGGATGCTCCCCTAATATCCATGTGCACAACTTGCCAGGGAAAGAATACAGCAAGGGAGATTCTCGATATGCAGCTAGGGAGTCCGGCATTGTCAGTATTGAAGGATTGACTTCTGTTTTAGAGGGTCCAGCTTGCATCTTAGCAGTGTAAGTATTTATCATCTGGAATTTAGAAATGCTATACGTATGAGGAATCATAATTAGACATAGGGCTGTTTGAATTGACTATTCTAAGTACTTGAAAGAGTGTTTCTAAGTAATCCAATTAGGGCCGTCAATGTGCTTTACTCTTCTTTTCCATTCCTTAACAATTTCGTTGGCCTGAATTGTTCACAGGTATGCAATAGCTACAGAAAAGTCGTACAGTTACATACTACAATTTGCCATATCATTGGGGCAGCTATATGGGACTTTTGTGTACTACATAACTGCTCTGTTAGATGGTGATAATTTTTCTTCAGGTCCATTTTATTACTATTCATACTTCGTAGTGGCAAATTCGTTCTGGATTCTTATTCCCTCGCTTATTGCTATGCGGTGTTGGAAGAAGATTTGTAAGGCAATAAGCATTGAAAGCCACATGAAAACCAAAATCCCCTAAGAAAGCTCTCAACGATTGATATCCCTTTGGGTTCATGAAAAATTGATGAAGTAGTACACAAAATTCTGGTTGTATTGTTGTGTGACATTCAGTAAACAAAGATATTCCTGGAATTTGAACATTTACATAAAGATTGGGTACGTTGATTCTGCACTTTTTGTGGTGTTGATTGAATTGACG

mRNA sequence

TCACAGGATGAAGCTCGAAGTGAAAGAACAGCGAGTTCATTGGTCAAACTTGAAACCAGAGGGAAAAAGAAGTCATCAATGGCGGGGGAATCTGAAGCTGCCCCGCATCCCTACGTTCCCACAGATCTGAAACTCCCCGAATATGTTCCTATTTCACTCCCTTTCTCTACGATTCTCGGCGTCTATCTCTTCTCTTCTCTTTTCGTCACTCTCCTCGTCTGGACCCTTTCCGGACGACTTACCAAAAAGACAAAGATTGAGAGGTTGCTCATGTGCTGGTGGATATTTACTGGCCTTACCCACATGGTTCTCGAGGGTTACTTTGTTTTCTCTCCAGAATTTTACAAGGACAAGACTTCTTTCTACCTTGCTGAAGTTTGGAAAGAATACAGCAAGGGAGATTCTCGATATGCAGCTAGGGAGTCCGGCATTGTCAGTATTGAAGGATTGACTTCTGTTTTAGAGGGTCCAGCTTGCATCTTAGCAGTGTATGCAATAGCTACAGAAAAGTCGTACAGTTACATACTACAATTTGCCATATCATTGGGGCAGCTATATGGGACTTTTGTGTACTACATAACTGCTCTGTTAGATGGTGATAATTTTTCTTCAGGTCCATTTTATTACTATTCATACTTCGTAGTGGCAAATTCGTTCTGGATTCTTATTCCCTCGCTTATTGCTATGCGGTGTTGGAAGAAGATTTGTAAGGCAATAAGCATTGAAAGCCACATGAAAACCAAAATCCCCTAAGAAAGCTCTCAACGATTGATATCCCTTTGGGTTCATGAAAAATTGATGAAGTAGTACACAAAATTCTGGTTGTATTGTTGTGTGACATTCAGTAAACAAAGATATTCCTGGAATTTGAACATTTACATAAAGATTGGGTACGTTGATTCTGCACTTTTTGTGGTGTTGATTGAATTGACG

Coding sequence (CDS)

TCACAGGATGAAGCTCGAAGTGAAAGAACAGCGAGTTCATTGGTCAAACTTGAAACCAGAGGGAAAAAGAAGTCATCAATGGCGGGGGAATCTGAAGCTGCCCCGCATCCCTACGTTCCCACAGATCTGAAACTCCCCGAATATGTTCCTATTTCACTCCCTTTCTCTACGATTCTCGGCGTCTATCTCTTCTCTTCTCTTTTCGTCACTCTCCTCGTCTGGACCCTTTCCGGACGACTTACCAAAAAGACAAAGATTGAGAGGTTGCTCATGTGCTGGTGGATATTTACTGGCCTTACCCACATGGTTCTCGAGGGTTACTTTGTTTTCTCTCCAGAATTTTACAAGGACAAGACTTCTTTCTACCTTGCTGAAGTTTGGAAAGAATACAGCAAGGGAGATTCTCGATATGCAGCTAGGGAGTCCGGCATTGTCAGTATTGAAGGATTGACTTCTGTTTTAGAGGGTCCAGCTTGCATCTTAGCAGTGTATGCAATAGCTACAGAAAAGTCGTACAGTTACATACTACAATTTGCCATATCATTGGGGCAGCTATATGGGACTTTTGTGTACTACATAACTGCTCTGTTAGATGGTGATAATTTTTCTTCAGGTCCATTTTATTACTATTCATACTTCGTAGTGGCAAATTCGTTCTGGATTCTTATTCCCTCGCTTATTGCTATGCGGTGTTGGAAGAAGATTTGTAAGGCAATAAGCATTGAAAGCCACATGAAAACCAAAATCCCCTAA

Protein sequence

SQDEARSERTASSLVKLETRGKKKSSMAGESEAAPHPYVPTDLKLPEYVPISLPFSTILGVYLFSSLFVTLLVWTLSGRLTKKTKIERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGLTSVLEGPACILAVYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSGPFYYYSYFVVANSFWILIPSLIAMRCWKKICKAISIESHMKTKIP
BLAST of ClCG07G012080 vs. Swiss-Prot
Match: EBP_ARATH (Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Arabidopsis thaliana GN=At1g20050 PE=2 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 1.9e-82
Identity = 143/218 (65.60%), Postives = 176/218 (80.73%), Query Frame = 1

Query: 32  EAAPHPYVPTDLKLPEYVPISLPFSTILGVYLFSSLFVTLLVWTLSGRLTKKTKIERLLM 91
           E   HPYVP DL LP YVPIS+  S+I+ +YL SSL V  LVW L GR  KK K+++LLM
Sbjct: 2   EELAHPYVPRDLNLPGYVPISMSMSSIVSIYLGSSLLVVSLVWLLFGR--KKAKLDKLLM 61

Query: 92  CWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGLT 151
           CWW FTGLTH++LEGYFVFSPEF+KD TS YLAEVWKEYSKGDSRY  R+S +VS+EG+T
Sbjct: 62  CWWTFTGLTHVILEGYFVFSPEFFKDNTSAYLAEVWKEYSKGDSRYVGRDSAVVSVEGIT 121

Query: 152 SVLEGPACILAVYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSGPFYYYS 211
           +V+ GPA +LA+YAIA EKSYSY+LQ AIS+ QLYG  VY+ITA+L+GDNF++  FYYYS
Sbjct: 122 AVIVGPASLLAIYAIAKEKSYSYVLQLAISVCQLYGCLVYFITAILEGDNFATNSFYYYS 181

Query: 212 YFVVANSFWILIPSLIAMRCWKKICKAISI-ESHMKTK 249
           Y++ AN +W+LIPSLI+ RCWKKIC A +I  ++++TK
Sbjct: 182 YYIGANCWWVLIPSLISFRCWKKICAAAAIANNNVETK 217

BLAST of ClCG07G012080 vs. Swiss-Prot
Match: EBP_ORYSJ (Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Oryza sativa subsp. japonica GN=Os01g0103600 PE=2 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 6.4e-70
Identity = 124/215 (57.67%), Postives = 162/215 (75.35%), Query Frame = 1

Query: 35  PHPYVPTDLKLPEYVPISLPFSTILGVYLFSSLFVTLLVWTLSGRLTKK-TKIERLLMCW 94
           PHPY P +L LP +VP+ L  + IL  YL +SLF+ L VW +SGR +++ +  +R LMCW
Sbjct: 6   PHPYAPAELHLPGFVPLQLSQAQILVPYLATSLFLLLAVWLISGRCSRRLSDTDRWLMCW 65

Query: 95  WIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGLTSV 154
           W FTGLTH+++EG FVF+P F+ ++   Y  EVWKEYSKGDSRY AR+   V++EG+T+V
Sbjct: 66  WAFTGLTHIIIEGTFVFAPNFFSNQNPSYFDEVWKEYSKGDSRYVARDPATVTVEGITAV 125

Query: 155 LEGPACILAVYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSGPFYYYSYF 214
           LEGPA +LAVYAIA+ KSYS+ILQF + LGQLYG  VY+ITA LDG NF + PFY+++YF
Sbjct: 126 LEGPASLLAVYAIASGKSYSHILQFTVCLGQLYGCLVYFITAYLDGFNFWTSPFYFWAYF 185

Query: 215 VVANSFWILIPSLIAMRCWKKICKAISIESHMKTK 249
           + ANS W++IP++IA+R WKKIC A   E  +KTK
Sbjct: 186 IGANSSWVVIPTMIAIRSWKKICAAFQGEK-VKTK 219

BLAST of ClCG07G012080 vs. Swiss-Prot
Match: EBP_RAT (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Rattus norvegicus GN=Ebp PE=2 SV=3)

HSP 1 Score: 145.6 bits (366), Expect = 7.3e-34
Identity = 78/221 (35.29%), Postives = 125/221 (56.56%), Query Frame = 1

Query: 36  HPYVPTDLKLPEYVPISLP-FSTILGVYLFSSLFVTLLVWTLSGRLTKKT--KIERLLMC 95
           HPY P  L+L  +VP  LP +  ++G++ FS + + ++ W LS R++        RL +C
Sbjct: 9   HPYWPRHLRLDNFVPNDLPTWHILVGLFSFSGVLI-VITWLLSSRVSVVPLGTGRRLALC 68

Query: 96  WWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGLTS 155
           W+      H+V+EG+F F  E   +  +F L+++WKEYSKGDSRY   +  IV +E +T+
Sbjct: 69  WFAVCTFIHLVIEGWFSFYHEILLEDQAF-LSQLWKEYSKGDSRYILSDGFIVCMESVTA 128

Query: 156 VLEGPACILAVYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSG----PFY 215
            L GP  +  V A    + + ++LQ  +S+GQ+YG  +Y++T L DG  F  G    P Y
Sbjct: 129 CLWGPLSLWVVIAFLRHQPFRFVLQLVVSVGQIYGDVLYFLTELRDG--FQHGELGHPLY 188

Query: 216 YYSYFVVANSFWILIPSLIAMRCWKKICKAISIESHMKTKI 250
           ++ YFV+ N+ W++IP ++     K +  A S+  +   KI
Sbjct: 189 FWFYFVIMNAIWLVIPGILVFDAIKHLTNAQSMLDNKVMKI 225

BLAST of ClCG07G012080 vs. Swiss-Prot
Match: EBP_MOUSE (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Mus musculus GN=Ebp PE=1 SV=3)

HSP 1 Score: 138.7 bits (348), Expect = 9.0e-32
Identity = 80/220 (36.36%), Postives = 125/220 (56.82%), Query Frame = 1

Query: 36  HPYVPTDLKLPEYVPISLPFSTILGVYLFS-SLFVTLLVWTLSGR--LTKKTKIERLLMC 95
           HPY P  LKL  +VP  LP S IL V LFS S  + ++ W LS R  +       RL +C
Sbjct: 9   HPYWPRHLKLDNFVPNDLPTSHIL-VGLFSISGGLIVITWLLSSRASVVPLGAGRRLALC 68

Query: 96  WWIFTGLTHMVLEGYF-VFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGLT 155
           W+      H+V+EG+F +++    +D+   +L+++WKEYSKGDSRY   +S +V +E +T
Sbjct: 69  WFAVCTFIHLVIEGWFSLYNGILLEDQA--FLSQLWKEYSKGDSRYILSDSFVVCMETVT 128

Query: 156 SVLEGPACILAVYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFS--SGPFYY 215
           + L GP  +  V A   ++ + ++LQ  +S+GQ+YG  +Y++T L +G        P Y+
Sbjct: 129 ACLWGPLSLWVVIAFLRQQPFRFVLQLVVSMGQIYGDVLYFLTELHEGLQHGEIGHPVYF 188

Query: 216 YSYFVVANSFWILIPSLIAMRCWKKICKAISIESHMKTKI 250
           + YFV  N+ W++IPS++ +   K +  A S+      KI
Sbjct: 189 WFYFVFLNAVWLVIPSILVLDAIKHLTSAQSVLDSKVMKI 225

BLAST of ClCG07G012080 vs. Swiss-Prot
Match: EBP_CAVPO (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cavia porcellus GN=EBP PE=1 SV=3)

HSP 1 Score: 134.0 bits (336), Expect = 2.2e-30
Identity = 79/209 (37.80%), Postives = 113/209 (54.07%), Query Frame = 1

Query: 27  MAGESEAAPHPYVPTDLKLPEYVPISLPFSTILGVY--LFSSLFVTLLVWTLSGR--LTK 86
           MA  S    HPY P  L+L  +VP  L    I+ V   +F +L VT+  W LS R  +  
Sbjct: 1   MATTSTGPLHPYWPRHLRLDHFVPNDLSAWYIVTVLFTVFGALVVTM--WLLSSRASVVP 60

Query: 87  KTKIERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARES 146
                RL +CW+      H+V+EG+FV   +      +F L+++WKEY+KGDSRY   ++
Sbjct: 61  LGTWRRLSVCWFAVCAFVHLVIEGWFVLYQKAILGDQAF-LSQLWKEYAKGDSRYIIEDN 120

Query: 147 GIVSIEGLTSVLEGPACILAVYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNF 206
            I+ +E +T VL GP  + AV A   +    Y+LQF ISLGQ+YG  +Y++T   DG  F
Sbjct: 121 FIICMESITVVLWGPLSLWAVIAFLRQHPSRYVLQFVISLGQIYGDLLYFLTEYRDG--F 180

Query: 207 SSG----PFYYYSYFVVANSFWILIPSLI 228
             G    P Y++ YF   N  W++IP ++
Sbjct: 181 QHGEMGHPIYFWFYFFFMNVLWLVIPGVL 204

BLAST of ClCG07G012080 vs. TrEMBL
Match: A0A0A0KVP4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G007050 PE=4 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 6.8e-111
Identity = 196/222 (88.29%), Postives = 209/222 (94.14%), Query Frame = 1

Query: 27  MAGESEAAPHPYVPTDLKLPEYVPISLPFSTILGVYLFSSLFVTLLVWTLSGRLTKKTKI 86
           MAG SEAAPHPYVPTDLKLP+YVPISLP STILG YL +SLF+ LLVWTLSGRLTKK KI
Sbjct: 1   MAGASEAAPHPYVPTDLKLPDYVPISLPLSTILGGYLLTSLFLVLLVWTLSGRLTKKAKI 60

Query: 87  ERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 146
           +RLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS
Sbjct: 61  DRLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 147 IEGLTSVLEGPACILAVYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSGP 206
           IEGLTSVLEGPACILA YAIA+ KSYSYILQFAISLGQLYGT +YY+TA+LDGD+FSSGP
Sbjct: 121 IEGLTSVLEGPACILAAYAIASGKSYSYILQFAISLGQLYGTLLYYVTAVLDGDDFSSGP 180

Query: 207 FYYYSYFVVANSFWILIPSLIAMRCWKKICKAISIESHMKTK 249
           FYYY+Y+VVANSFWILIPSLI +RCWKKIC A+SIES  KTK
Sbjct: 181 FYYYAYYVVANSFWILIPSLIGIRCWKKICIAMSIESQTKTK 222

BLAST of ClCG07G012080 vs. TrEMBL
Match: A0A067KYV5_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_08286 PE=4 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 5.4e-92
Identity = 158/217 (72.81%), Postives = 187/217 (86.18%), Query Frame = 1

Query: 32  EAAPHPYVPTDLKLPEYVPISLPFSTILGVYLFSSLFVTLLVWTLSGRLTKKTKIERLLM 91
           E  PHPYVP DLKLP+YVP+ LP STIL VY  SSL V  LVW LSGRL+K  KI+R+LM
Sbjct: 2   EEQPHPYVPRDLKLPDYVPVLLPQSTILSVYGISSLVVVSLVWILSGRLSKTAKIDRILM 61

Query: 92  CWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGLT 151
           CWW FTGLTH++LEGYF FSPEFYK+KT FYLAEVWKEYSKGDSRYAAR++GIVS+EGLT
Sbjct: 62  CWWAFTGLTHVILEGYFAFSPEFYKEKTPFYLAEVWKEYSKGDSRYAARDAGIVSVEGLT 121

Query: 152 SVLEGPACILAVYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSGPFYYYS 211
           +VLEGPA +LAVYAIAT+KSYSYILQFAISLGQLYGT VY+ITA L+GDNF++ P+YYY+
Sbjct: 122 AVLEGPASLLAVYAIATKKSYSYILQFAISLGQLYGTAVYFITAFLEGDNFAASPYYYYA 181

Query: 212 YFVVANSFWILIPSLIAMRCWKKICKAISIESHMKTK 249
           Y++ AN+ W++IPS+I++RCWKKIC A   +   KTK
Sbjct: 182 YYIAANASWVVIPSIISIRCWKKICAAFEAQGQKKTK 218

BLAST of ClCG07G012080 vs. TrEMBL
Match: A0A0B0NQY0_GOSAR (Uncharacterized protein OS=Gossypium arboreum GN=F383_05106 PE=4 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 6.6e-90
Identity = 159/214 (74.30%), Postives = 181/214 (84.58%), Query Frame = 1

Query: 36  HPYVPTDLKLPEYVPISLPFSTILGVYLFSSLFVTLLVWTLSGRLTKKTKIERLLMCWWI 95
           HPYVP DL+LP YVP+SL  STIL VY  SSL V  LVW LSGR    +K++RLLMCWW 
Sbjct: 3   HPYVPRDLQLPGYVPVSLSQSTILTVYGLSSLLVVSLVWFLSGRSRNISKLDRLLMCWWA 62

Query: 96  FTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGLTSVLE 155
           FTGLTH++LEGYF FSPEFYKDKT FYLAEVWKEYSKGDSRYA R+S IV++EG+TSVLE
Sbjct: 63  FTGLTHLILEGYFAFSPEFYKDKTGFYLAEVWKEYSKGDSRYAGRDSAIVAVEGMTSVLE 122

Query: 156 GPACILAVYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSGPFYYYSYFVV 215
           GP C+LAVYAIA  K YSYILQFAISLGQLYGTFVY+ITA L+GDNFS+ PFYYY+Y+V+
Sbjct: 123 GPPCLLAVYAIAKGKGYSYILQFAISLGQLYGTFVYFITAYLEGDNFSASPFYYYAYYVL 182

Query: 216 ANSFWILIPSLIAMRCWKKICKAISIESHMKTKI 250
           ANSFW+LIPSLIA+RCWKKI  A+  +S  K KI
Sbjct: 183 ANSFWLLIPSLIAIRCWKKISSAVQSQSQKKNKI 216

BLAST of ClCG07G012080 vs. TrEMBL
Match: A0A0D2RMS0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_006G002800 PE=4 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 8.6e-90
Identity = 159/214 (74.30%), Postives = 181/214 (84.58%), Query Frame = 1

Query: 36  HPYVPTDLKLPEYVPISLPFSTILGVYLFSSLFVTLLVWTLSGRLTKKTKIERLLMCWWI 95
           HPYVP DL+LP YVP+SL  STIL VY  SSL V  LVW LSGR    +K++RLLMCWW 
Sbjct: 3   HPYVPRDLQLPGYVPVSLSQSTILTVYGLSSLLVVSLVWFLSGRSRSISKLDRLLMCWWA 62

Query: 96  FTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGLTSVLE 155
           FTGLTH++LEGYF FSPEFYKDKT FYLAEVWKEYSKGDSRYA R+S IV++EG+TSVLE
Sbjct: 63  FTGLTHIILEGYFAFSPEFYKDKTGFYLAEVWKEYSKGDSRYAGRDSAIVAVEGMTSVLE 122

Query: 156 GPACILAVYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSGPFYYYSYFVV 215
           GP C+LAVYAIA  K YSYILQFAISLGQLYGTFVY+ITA L+GDNFS+ PFYYY+Y+V+
Sbjct: 123 GPPCLLAVYAIAKGKGYSYILQFAISLGQLYGTFVYFITAYLEGDNFSASPFYYYAYYVL 182

Query: 216 ANSFWILIPSLIAMRCWKKICKAISIESHMKTKI 250
           ANSFW+LIPSLIA+RCWKKI  A+  +S  K KI
Sbjct: 183 ANSFWLLIPSLIAIRCWKKISSAVQSQSQKKNKI 216

BLAST of ClCG07G012080 vs. TrEMBL
Match: D6PY90_GOSHI (Putative C-8,7 sterol isomerase OS=Gossypium hirsutum GN=HYDRA1 PE=2 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 1.5e-89
Identity = 159/214 (74.30%), Postives = 181/214 (84.58%), Query Frame = 1

Query: 36  HPYVPTDLKLPEYVPISLPFSTILGVYLFSSLFVTLLVWTLSGRLTKKTKIERLLMCWWI 95
           HPYVP DL+LP YVP+SL  STIL VY  SSL V  LVW LSGR    +K++RLLMCWW 
Sbjct: 3   HPYVPRDLQLPGYVPVSLSQSTILTVYGLSSLLVVSLVWFLSGRSRSISKLDRLLMCWWA 62

Query: 96  FTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGLTSVLE 155
           FTGLTH++LEGYF FSPEFYKDKT FYLAEVWKEYSKGDSRYA R+S IV++EG+TSVLE
Sbjct: 63  FTGLTHIILEGYFAFSPEFYKDKTGFYLAEVWKEYSKGDSRYAGRDSTIVAVEGITSVLE 122

Query: 156 GPACILAVYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSGPFYYYSYFVV 215
           GP C+LAVYAIA  K YSYILQFAISLGQLYGTFVY+ITA L+GDNFS+ PFYYY+Y+V+
Sbjct: 123 GPPCLLAVYAIAKGKGYSYILQFAISLGQLYGTFVYFITAYLEGDNFSASPFYYYAYYVL 182

Query: 216 ANSFWILIPSLIAMRCWKKICKAISIESHMKTKI 250
           ANSFW+LIPSLIA+RCWKKI  A+  +S  K KI
Sbjct: 183 ANSFWLLIPSLIAIRCWKKISSAVQSQSQKKNKI 216

BLAST of ClCG07G012080 vs. TAIR10
Match: AT1G20050.1 (AT1G20050.1 C-8,7 sterol isomerase)

HSP 1 Score: 307.0 bits (785), Expect = 1.1e-83
Identity = 143/218 (65.60%), Postives = 176/218 (80.73%), Query Frame = 1

Query: 32  EAAPHPYVPTDLKLPEYVPISLPFSTILGVYLFSSLFVTLLVWTLSGRLTKKTKIERLLM 91
           E   HPYVP DL LP YVPIS+  S+I+ +YL SSL V  LVW L GR  KK K+++LLM
Sbjct: 2   EELAHPYVPRDLNLPGYVPISMSMSSIVSIYLGSSLLVVSLVWLLFGR--KKAKLDKLLM 61

Query: 92  CWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGLT 151
           CWW FTGLTH++LEGYFVFSPEF+KD TS YLAEVWKEYSKGDSRY  R+S +VS+EG+T
Sbjct: 62  CWWTFTGLTHVILEGYFVFSPEFFKDNTSAYLAEVWKEYSKGDSRYVGRDSAVVSVEGIT 121

Query: 152 SVLEGPACILAVYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSGPFYYYS 211
           +V+ GPA +LA+YAIA EKSYSY+LQ AIS+ QLYG  VY+ITA+L+GDNF++  FYYYS
Sbjct: 122 AVIVGPASLLAIYAIAKEKSYSYVLQLAISVCQLYGCLVYFITAILEGDNFATNSFYYYS 181

Query: 212 YFVVANSFWILIPSLIAMRCWKKICKAISI-ESHMKTK 249
           Y++ AN +W+LIPSLI+ RCWKKIC A +I  ++++TK
Sbjct: 182 YYIGANCWWVLIPSLISFRCWKKICAAAAIANNNVETK 217

BLAST of ClCG07G012080 vs. NCBI nr
Match: gi|659108366|ref|XP_008454161.1| (PREDICTED: probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucumis melo])

HSP 1 Score: 412.1 bits (1058), Expect = 6.7e-112
Identity = 198/222 (89.19%), Postives = 210/222 (94.59%), Query Frame = 1

Query: 27  MAGESEAAPHPYVPTDLKLPEYVPISLPFSTILGVYLFSSLFVTLLVWTLSGRLTKKTKI 86
           MAG SEAAPHPYVPTDLKLP+YVPISLP STILG YL SSLF+ LL+WTLSGRLTKKTKI
Sbjct: 1   MAGASEAAPHPYVPTDLKLPDYVPISLPLSTILGGYLLSSLFLVLLLWTLSGRLTKKTKI 60

Query: 87  ERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 146
           +RLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIV 
Sbjct: 61  DRLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVG 120

Query: 147 IEGLTSVLEGPACILAVYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSGP 206
           IEGLTSVLEGPACILA YAIA+ KSYSYILQFAISLGQLYGT +YYITA+LDGD+FSSGP
Sbjct: 121 IEGLTSVLEGPACILAAYAIASGKSYSYILQFAISLGQLYGTLLYYITAVLDGDDFSSGP 180

Query: 207 FYYYSYFVVANSFWILIPSLIAMRCWKKICKAISIESHMKTK 249
            YYYSY+VVANSFW+LIPSLIA+RCWKKIC A+SIES MKTK
Sbjct: 181 IYYYSYYVVANSFWLLIPSLIAIRCWKKICNAMSIESQMKTK 222

BLAST of ClCG07G012080 vs. NCBI nr
Match: gi|449468918|ref|XP_004152168.1| (PREDICTED: probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucumis sativus])

HSP 1 Score: 408.3 bits (1048), Expect = 9.7e-111
Identity = 196/222 (88.29%), Postives = 209/222 (94.14%), Query Frame = 1

Query: 27  MAGESEAAPHPYVPTDLKLPEYVPISLPFSTILGVYLFSSLFVTLLVWTLSGRLTKKTKI 86
           MAG SEAAPHPYVPTDLKLP+YVPISLP STILG YL +SLF+ LLVWTLSGRLTKK KI
Sbjct: 1   MAGASEAAPHPYVPTDLKLPDYVPISLPLSTILGGYLLTSLFLVLLVWTLSGRLTKKAKI 60

Query: 87  ERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 146
           +RLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS
Sbjct: 61  DRLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 147 IEGLTSVLEGPACILAVYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSGP 206
           IEGLTSVLEGPACILA YAIA+ KSYSYILQFAISLGQLYGT +YY+TA+LDGD+FSSGP
Sbjct: 121 IEGLTSVLEGPACILAAYAIASGKSYSYILQFAISLGQLYGTLLYYVTAVLDGDDFSSGP 180

Query: 207 FYYYSYFVVANSFWILIPSLIAMRCWKKICKAISIESHMKTK 249
           FYYY+Y+VVANSFWILIPSLI +RCWKKIC A+SIES  KTK
Sbjct: 181 FYYYAYYVVANSFWILIPSLIGIRCWKKICIAMSIESQTKTK 222

BLAST of ClCG07G012080 vs. NCBI nr
Match: gi|802600309|ref|XP_012072878.1| (PREDICTED: probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Jatropha curcas])

HSP 1 Score: 345.5 bits (885), Expect = 7.7e-92
Identity = 158/217 (72.81%), Postives = 187/217 (86.18%), Query Frame = 1

Query: 32  EAAPHPYVPTDLKLPEYVPISLPFSTILGVYLFSSLFVTLLVWTLSGRLTKKTKIERLLM 91
           E  PHPYVP DLKLP+YVP+ LP STIL VY  SSL V  LVW LSGRL+K  KI+R+LM
Sbjct: 2   EEQPHPYVPRDLKLPDYVPVLLPQSTILSVYGISSLVVVSLVWILSGRLSKTAKIDRILM 61

Query: 92  CWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGLT 151
           CWW FTGLTH++LEGYF FSPEFYK+KT FYLAEVWKEYSKGDSRYAAR++GIVS+EGLT
Sbjct: 62  CWWAFTGLTHVILEGYFAFSPEFYKEKTPFYLAEVWKEYSKGDSRYAARDAGIVSVEGLT 121

Query: 152 SVLEGPACILAVYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSGPFYYYS 211
           +VLEGPA +LAVYAIAT+KSYSYILQFAISLGQLYGT VY+ITA L+GDNF++ P+YYY+
Sbjct: 122 AVLEGPASLLAVYAIATKKSYSYILQFAISLGQLYGTAVYFITAFLEGDNFAASPYYYYA 181

Query: 212 YFVVANSFWILIPSLIAMRCWKKICKAISIESHMKTK 249
           Y++ AN+ W++IPS+I++RCWKKIC A   +   KTK
Sbjct: 182 YYIAANASWVVIPSIISIRCWKKICAAFEAQGQKKTK 218

BLAST of ClCG07G012080 vs. NCBI nr
Match: gi|728834048|gb|KHG13491.1| (hypothetical protein F383_05106 [Gossypium arboreum])

HSP 1 Score: 338.6 bits (867), Expect = 9.4e-90
Identity = 159/214 (74.30%), Postives = 181/214 (84.58%), Query Frame = 1

Query: 36  HPYVPTDLKLPEYVPISLPFSTILGVYLFSSLFVTLLVWTLSGRLTKKTKIERLLMCWWI 95
           HPYVP DL+LP YVP+SL  STIL VY  SSL V  LVW LSGR    +K++RLLMCWW 
Sbjct: 3   HPYVPRDLQLPGYVPVSLSQSTILTVYGLSSLLVVSLVWFLSGRSRNISKLDRLLMCWWA 62

Query: 96  FTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGLTSVLE 155
           FTGLTH++LEGYF FSPEFYKDKT FYLAEVWKEYSKGDSRYA R+S IV++EG+TSVLE
Sbjct: 63  FTGLTHLILEGYFAFSPEFYKDKTGFYLAEVWKEYSKGDSRYAGRDSAIVAVEGMTSVLE 122

Query: 156 GPACILAVYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSGPFYYYSYFVV 215
           GP C+LAVYAIA  K YSYILQFAISLGQLYGTFVY+ITA L+GDNFS+ PFYYY+Y+V+
Sbjct: 123 GPPCLLAVYAIAKGKGYSYILQFAISLGQLYGTFVYFITAYLEGDNFSASPFYYYAYYVL 182

Query: 216 ANSFWILIPSLIAMRCWKKICKAISIESHMKTKI 250
           ANSFW+LIPSLIA+RCWKKI  A+  +S  K KI
Sbjct: 183 ANSFWLLIPSLIAIRCWKKISSAVQSQSQKKNKI 216

BLAST of ClCG07G012080 vs. NCBI nr
Match: gi|823166801|ref|XP_012483353.1| (PREDICTED: probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Gossypium raimondii])

HSP 1 Score: 338.2 bits (866), Expect = 1.2e-89
Identity = 159/214 (74.30%), Postives = 181/214 (84.58%), Query Frame = 1

Query: 36  HPYVPTDLKLPEYVPISLPFSTILGVYLFSSLFVTLLVWTLSGRLTKKTKIERLLMCWWI 95
           HPYVP DL+LP YVP+SL  STIL VY  SSL V  LVW LSGR    +K++RLLMCWW 
Sbjct: 3   HPYVPRDLQLPGYVPVSLSQSTILTVYGLSSLLVVSLVWFLSGRSRSISKLDRLLMCWWA 62

Query: 96  FTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGLTSVLE 155
           FTGLTH++LEGYF FSPEFYKDKT FYLAEVWKEYSKGDSRYA R+S IV++EG+TSVLE
Sbjct: 63  FTGLTHIILEGYFAFSPEFYKDKTGFYLAEVWKEYSKGDSRYAGRDSAIVAVEGMTSVLE 122

Query: 156 GPACILAVYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSGPFYYYSYFVV 215
           GP C+LAVYAIA  K YSYILQFAISLGQLYGTFVY+ITA L+GDNFS+ PFYYY+Y+V+
Sbjct: 123 GPPCLLAVYAIAKGKGYSYILQFAISLGQLYGTFVYFITAYLEGDNFSASPFYYYAYYVL 182

Query: 216 ANSFWILIPSLIAMRCWKKICKAISIESHMKTKI 250
           ANSFW+LIPSLIA+RCWKKI  A+  +S  K KI
Sbjct: 183 ANSFWLLIPSLIAIRCWKKISSAVQSQSQKKNKI 216

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
EBP_ARATH1.9e-8265.60Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Arabidopsis thalia... [more]
EBP_ORYSJ6.4e-7057.67Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Oryza sativa subsp... [more]
EBP_RAT7.3e-3435.293-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Rattus norvegicus GN=Ebp PE... [more]
EBP_MOUSE9.0e-3236.363-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Mus musculus GN=Ebp PE=1 SV... [more]
EBP_CAVPO2.2e-3037.803-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cavia porcellus GN=EBP PE=1... [more]
Match NameE-valueIdentityDescription
A0A0A0KVP4_CUCSA6.8e-11188.29Uncharacterized protein OS=Cucumis sativus GN=Csa_4G007050 PE=4 SV=1[more]
A0A067KYV5_JATCU5.4e-9272.81Uncharacterized protein OS=Jatropha curcas GN=JCGZ_08286 PE=4 SV=1[more]
A0A0B0NQY0_GOSAR6.6e-9074.30Uncharacterized protein OS=Gossypium arboreum GN=F383_05106 PE=4 SV=1[more]
A0A0D2RMS0_GOSRA8.6e-9074.30Uncharacterized protein OS=Gossypium raimondii GN=B456_006G002800 PE=4 SV=1[more]
D6PY90_GOSHI1.5e-8974.30Putative C-8,7 sterol isomerase OS=Gossypium hirsutum GN=HYDRA1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G20050.11.1e-8365.60 C-8,7 sterol isomerase[more]
Match NameE-valueIdentityDescription
gi|659108366|ref|XP_008454161.1|6.7e-11289.19PREDICTED: probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucumis m... [more]
gi|449468918|ref|XP_004152168.1|9.7e-11188.29PREDICTED: probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucumis s... [more]
gi|802600309|ref|XP_012072878.1|7.7e-9272.81PREDICTED: probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Jatropha ... [more]
gi|728834048|gb|KHG13491.1|9.4e-9074.30hypothetical protein F383_05106 [Gossypium arboreum][more]
gi|823166801|ref|XP_012483353.1|1.2e-8974.30PREDICTED: probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Gossypium... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR007905EBP
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
Vocabulary: Biological Process
TermDefinition
GO:0016125sterol metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0047750cholestenol delta-isomerase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0060964 regulation of gene silencing by miRNA
biological_process GO:0006694 steroid biosynthetic process
biological_process GO:0016125 sterol metabolic process
biological_process GO:0006084 acetyl-CoA metabolic process
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0019932 second-messenger-mediated signaling
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005622 intracellular
cellular_component GO:0016020 membrane
molecular_function GO:0000247 C-8 sterol isomerase activity
molecular_function GO:0047750 cholestenol delta-isomerase activity
molecular_function GO:0016853 isomerase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG07G012080.1ClCG07G012080.1mRNA


Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007905Emopamil-binding proteinPANTHERPTHR14207STEROL ISOMERASEcoord: 23..248
score: 1.4E
IPR007905Emopamil-binding proteinPFAMPF05241EBPcoord: 63..238
score: 1.6
NoneNo IPR availablePANTHERPTHR14207:SF03-BETA-HYDROXYSTEROID-DELTA(8),DELTA(7)-ISOMERASEcoord: 23..248
score: 1.4E