CmoCh14G014230 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh14G014230
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	3-beta-hydroxysteroid-delta-isomerase
Location	Cmo_Chr14 : 11601198 .. 11603965 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAACTGAGAGGACAGGGAGTTCATTTGGTCAAACTTGAAACCAGAGGAAAAAGAGAGTCGCCAATGGCGGGGGAATCTGAAGCTGCTCGGCATCCCTACGCACCCACAGATCTGGAACTCCCCGGATATGTTCCTATTTCACTACCCCTCACTACTATTCTCGGCGTTTATGTCCTCTCTTCTCTCTTTATCACTTTGCTGCTCTGGACTCTTTCCGGTATGCTTTCATCTCTTACTTTGATATTTACTGTCGTTTTTTTGGATTCTGTTTTATGCGCTTCGAATTCTGCTTGATTTCTTGCTGGAGAAGAAGACCGATGGTTTTCATAATCCGTAGTTTGTTGTTGTGTTGGATTTCTGTCCACTTGTTGAGTGAAGTAATTGGAAGCAAAATCAAACAATTTCAAATATTCTATGTTTTTCGTGTCCTCCAAGAATTCTATTGAATGTCTTTGCTGAATCTGAAGGCTGGCGATGAAACCGATGTAAAAATTTAAATCTGTCTCGTTCAATTATTCTTTTCGAGAGGATCGAGATCTGTTAAACGGATAGACGCCAGATTTCCCTTGCCTTGCCTTCCTACTCCTTAACTTGTGCTATTGTCTTTTGGTTTAGATGATTGCTTTAGAAAGGGAATACTGATGGGACGGACTAAGCGAGGATGTCTATGATTATACCTGAGTGTGAAAGAATGAAACATGTAGCAAACTGAAATCTATTTGGCCATTTATGGTTCTGTCAATCCGCGTTTGACTTCATTTGATTGGTTAATTTTGACACGATACAATATCTATTTTAGATTTAAACTCTTGATGTTTCCTTTGGTTATGCATACTCCCCTTTGTCTAAACAATCATTCATCCATACACTTTCTACTAATGAGGAACATAAATTTATCTAGATTTCAGAATGCTATGAGGATGAAATGTTAATGTTAAGATTACTATTTGGATATCAGTGATTAGCTTGTAAGTTTGTTTGTGTTGAGTGGTTATGGATATAGTGGGTTGAGAGAGTGAAAGAGGGGCAGATTTGTATAGTTTTGGAAAGAGTACTAGGCCTCTCGTAGAGCTTGGAACCTTGTAATTTGTCTAATATCAATATAATATTTCAATTAACCTGTCCAATTTTTGGTTCCTATCAAATTAGTTCCACTACATTTGTGCTCGAAATTGAATCTTTTCAAGTCTAGAAGAATATTGATGAACATGTTGTTCACAAATGGATCTTTTTCTTTTTAGGGCAGATATAGGGATGTTCATTTTGGGGTCCTTGTCCTTCGAAGTGTTTTTTCTTGTAGCTTGAGCCATCCCTCTCCAATGGTTCCACTTTTTTCCTTAGTGTAGGTAGAGTGTTGTACAAACACTAGATCCTAACACCATGATTGAAGTATGAAACACCTGATAATGGGCACTATACTATATTCAAACACTTAGTGTTGGTAGAGTGTTGTACAAGCACTTATGTTGCAAGCTATGACATATTTTGCTTTCCTATTGCACCCTGTGTTTTTTTCATGTTCCCTTCCGTGGTGTTTGTACACTTGTTCTCTCCATCTTTATCTATTTCTTTTTATCAGAATTTAGTAATGGAAAAAACTGTAGTTCTTAGAGTTGTTTGGAAAAGGTTCTATGCTTGTTGCTTTATCTTCATTTCCCCCTTATAACAGGACGACTTCCCAAAAAAACAAAGATTGAGAGGCTGCTCATGTGCTGGTGGATATTTACGGGCCTTACCCACATTGTTCTCGAGGGTTACTTTGTTTTCTCTCCAGAATTTTACAAGGACGGTTCTTACCTTGCTGAAGTTTGTAAGTTAATTTAATTCACTTTCTTTGTTACCCTAGCTGTGGTACTGGAAGGGCTGTATGGATGCTCCTCTAATAATGTTCATGTGCACTACTTACCAGGGAAAGAATACAGCAAGGGAGATTCTCGATACGCAACTCGGGACTCTGCGATTGTCAGTCTTGAAGGACTGACTGCTGTTTTAGAGGGTCCAGCTTGCATCTTAGCAGTGTAAGAATTTATCATCTCTGGGGTTTTGGGAGAGAATAGTTAATCAATTTCTAAATGACTAAGATACAAGAAGTTATGATACAAAACAAATACAAAAGAGGTAACTAGCTAATAAAATCTAAACCAAAGAATTTTGAAATGCTATACATATAAGGAATTACATCTGAACTTTCTGTTCATAATTAGCCATTCATCAACAAATTTGTTGGGCTGTGTATAATTCGCAGGTATGCAATAGCTACAGACAAGTCGTACAGTTATGTACTGCAGTTCGCCATATCATTGGGACAGCTATATGGAACTTTCATGTACTACATAACTGCTCTGTTGGACGGTGATAATTTTTCTTCATCCCCAATTTATTACTATTTATACTACGTATTGTTAAATTCCTTCTGGATTCTTATTCCTTCGCTTATTGCTATCCGGTGTTGGAAGAAAATTTGTATTGCTATGAGCAGTGAAAGCCACAAGAAAACCAAAGTTCCCTAAGAAGTGATCCATATTATGACGTCTACCCCTTTGGATTCATGGAAAATAATAGATTAGATAGTACACAAGATTCTTGTTGTATTGTAATCTAAGTTATTGTCGTGTGACATTCAGAATACGAGACACTCGTGGAATTTGAACATTTACACGAAGTTTGGGTTTGTTGATTCTGCCTTTTTTGGGTGTTAAATTAACGATGTCGATTCGAATTTGTTGTTGGTTATTTCATCAACTTTTTAGCATATTATTAGTAAAGCG

mRNA sequence

GAACTGAGAGGACAGGGAGTTCATTTGGTCAAACTTGAAACCAGAGGAAAAAGAGAGTCGCCAATGGCGGGGGAATCTGAAGCTGCTCGGCATCCCTACGCACCCACAGATCTGGAACTCCCCGGATATGTTCCTATTTCACTACCCCTCACTACTATTCTCGGCGTTTATGTCCTCTCTTCTCTCTTTATCACTTTGCTGCTCTGGACTCTTTCCGGACGACTTCCCAAAAAAACAAAGATTGAGAGGCTGCTCATGTGCTGGTGGATATTTACGGGCCTTACCCACATTGTTCTCGAGGGTTACTTTGTTTTCTCTCCAGAATTTTACAAGGACGGTTCTTACCTTGCTGAAGTTTGGAAAGAATACAGCAAGGGAGATTCTCGATACGCAACTCGGGACTCTGCGATTGTCAGTCTTGAAGGACTGACTGCTGTTTTAGAGGGTCCAGCTTGCATCTTAGCAGTGTATGCAATAGCTACAGACAAGTCGTACAGTTATGTACTGCAGTTCGCCATATCATTGGGACAGCTATATGGAACTTTCATGTACTACATAACTGCTCTGTTGGACGGTGATAATTTTTCTTCATCCCCAATTTATTACTATTTATACTACGTATTGTTAAATTCCTTCTGGATTCTTATTCCTTCGCTTATTGCTATCCGGTGTTGGAAGAAAATTTGTATTGCTATGAGCAGTGAAAGCCACAAGAAAACCAAAGTTCCCTAAGAAGTGATCCATATTATGACGTCTACCCCTTTGGATTCATGGAAAATAATAGATTAGATAGTACACAAGATTCTTGTTGTATTGTAATCTAAGTTATTGTCGTGTGACATTCAGAATACGAGACACTCGTGGAATTTGAACATTTACACGAAGTTTGGGTTTGTTGATTCTGCCTTTTTTGGGTGTTAAATTAACGATGTCGATTCGAATTTGTTGTTGGTTATTTCATCAACTTTTTAGCATATTATTAGTAAAGCG

Coding sequence (CDS)

ATGGCGGGGGAATCTGAAGCTGCTCGGCATCCCTACGCACCCACAGATCTGGAACTCCCCGGATATGTTCCTATTTCACTACCCCTCACTACTATTCTCGGCGTTTATGTCCTCTCTTCTCTCTTTATCACTTTGCTGCTCTGGACTCTTTCCGGACGACTTCCCAAAAAAACAAAGATTGAGAGGCTGCTCATGTGCTGGTGGATATTTACGGGCCTTACCCACATTGTTCTCGAGGGTTACTTTGTTTTCTCTCCAGAATTTTACAAGGACGGTTCTTACCTTGCTGAAGTTTGGAAAGAATACAGCAAGGGAGATTCTCGATACGCAACTCGGGACTCTGCGATTGTCAGTCTTGAAGGACTGACTGCTGTTTTAGAGGGTCCAGCTTGCATCTTAGCAGTGTATGCAATAGCTACAGACAAGTCGTACAGTTATGTACTGCAGTTCGCCATATCATTGGGACAGCTATATGGAACTTTCATGTACTACATAACTGCTCTGTTGGACGGTGATAATTTTTCTTCATCCCCAATTTATTACTATTTATACTACGTATTGTTAAATTCCTTCTGGATTCTTATTCCTTCGCTTATTGCTATCCGGTGTTGGAAGAAAATTTGTATTGCTATGAGCAGTGAAAGCCACAAGAAAACCAAAGTTCCCTAA

Homology

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BLAST of CmoCh14G014230 vs. Swiss-Prot
Match: EBP_ARATH (Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Arabidopsis thaliana GN=At1g20050 PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 2.9e-74
Identity = 137/224 (61.16%), Postives = 177/224 (79.02%), Query Frame = 1


Query: 6   EAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKIERLLM 65
           E   HPY P DL LPGYVPIS+ +++I+ +Y+ SSL +  L+W L GR  KK K+++LLM
Sbjct: 2   EELAHPYVPRDLNLPGYVPISMSMSSIVSIYLGSSLLVVSLVWLLFGR--KKAKLDKLLM 61

Query: 66  CWWIFTGLTHIVLEGYFVFSPEFYKDGS--YLAEVWKEYSKGDSRYATRDSAIVSLEGLT 125
           CWW FTGLTH++LEGYFVFSPEF+KD +  YLAEVWKEYSKGDSRY  RDSA+VS+EG+T
Sbjct: 62  CWWTFTGLTHVILEGYFVFSPEFFKDNTSAYLAEVWKEYSKGDSRYVGRDSAVVSVEGIT 121

Query: 126 AVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIYYYL 185
           AV+ GPA +LA+YAIA +KSYSYVLQ AIS+ QLYG  +Y+ITA+L+GDNF+++  YYY 
Sbjct: 122 AVIVGPASLLAIYAIAKEKSYSYVLQLAISVCQLYGCLVYFITAILEGDNFATNSFYYYS 181

Query: 186 YYVLLNSFWILIPSLIAIRCWKKICIA-------MSSESHKKTK 221
           YY+  N +W+LIPSLI+ RCWKKIC A       + +++ KKT+
Sbjct: 182 YYIGANCWWVLIPSLISFRCWKKICAAAAIANNNVETKTKKKTR 223

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BLAST of CmoCh14G014230 vs. Swiss-Prot
Match: EBP_ORYSJ (Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Oryza sativa subsp. japonica GN=Os01g0103600 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 9.3e-65
Identity = 122/211 (57.82%), Postives = 159/211 (75.36%), Query Frame = 1


Query: 10  HPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKK-TKIERLLMCWW 69
           HPYAP +L LPG+VP+ L    IL  Y+ +SLF+ L +W +SGR  ++ +  +R LMCWW
Sbjct: 7   HPYAPAELHLPGFVPLQLSQAQILVPYLATSLFLLLAVWLISGRCSRRLSDTDRWLMCWW 66

Query: 70  IFTGLTHIVLEGYFVFSPEFY--KDGSYLAEVWKEYSKGDSRYATRDSAIVSLEGLTAVL 129
            FTGLTHI++EG FVF+P F+  ++ SY  EVWKEYSKGDSRY  RD A V++EG+TAVL
Sbjct: 67  AFTGLTHIIIEGTFVFAPNFFSNQNPSYFDEVWKEYSKGDSRYVARDPATVTVEGITAVL 126

Query: 130 EGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIYYYLYYV 189
           EGPA +LAVYAIA+ KSYS++LQF + LGQLYG  +Y+ITA LDG NF +SP Y++ Y++
Sbjct: 127 EGPASLLAVYAIASGKSYSHILQFTVCLGQLYGCLVYFITAYLDGFNFWTSPFYFWAYFI 186

Query: 190 LLNSFWILIPSLIAIRCWKKICIAMSSESHK 218
             NS W++IP++IAIR WKKIC A   E  K
Sbjct: 187 GANSSWVVIPTMIAIRSWKKICAAFQGEKVK 217

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BLAST of CmoCh14G014230 vs. Swiss-Prot
Match: EBP_RAT (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Rattus norvegicus GN=Ebp PE=2 SV=3)

HSP 1 Score: 137.9 bits (346), Expect = 1.4e-31
Identity = 82/219 (37.44%), Postives = 126/219 (57.53%), Query Frame = 1


Query: 10  HPYAPTDLELPGYVPISLPLTTIL-GVYVLSSLFITLLLWTLSGRL---PKKTKIERLLM 69
           HPY P  L L  +VP  LP   IL G++  S + I ++ W LS R+   P  T   RL +
Sbjct: 9   HPYWPRHLRLDNFVPNDLPTWHILVGLFSFSGVLI-VITWLLSSRVSVVPLGTG-RRLAL 68

Query: 70  CWWIFTGLTHIVLEGYFVFSPEFY-KDGSYLAEVWKEYSKGDSRYATRDSAIVSLEGLTA 129
           CW+      H+V+EG+F F  E   +D ++L+++WKEYSKGDSRY   D  IV +E +TA
Sbjct: 69  CWFAVCTFIHLVIEGWFSFYHEILLEDQAFLSQLWKEYSKGDSRYILSDGFIVCMESVTA 128

Query: 130 VLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFS--SSPIYYY 189
            L GP  +  V A    + + +VLQ  +S+GQ+YG  +Y++T L DG        P+Y++
Sbjct: 129 CLWGPLSLWVVIAFLRHQPFRFVLQLVVSVGQIYGDVLYFLTELRDGFQHGELGHPLYFW 188

Query: 190 LYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKV 222
            Y+V++N+ W++IP ++     K +  A S   +K  K+
Sbjct: 189 FYFVIMNAIWLVIPGILVFDAIKHLTNAQSMLDNKVMKI 225

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BLAST of CmoCh14G014230 vs. Swiss-Prot
Match: EBP_MOUSE (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Mus musculus GN=Ebp PE=1 SV=3)

HSP 1 Score: 134.8 bits (338), Expect = 1.1e-30
Identity = 79/218 (36.24%), Postives = 128/218 (58.72%), Query Frame = 1


Query: 10  HPYAPTDLELPGYVPISLPLTTIL-GVYVLSSLFITLLLWTLSGR--LPKKTKIERLLMC 69
           HPY P  L+L  +VP  LP + IL G++ +S   I ++ W LS R  +       RL +C
Sbjct: 9   HPYWPRHLKLDNFVPNDLPTSHILVGLFSISGGLI-VITWLLSSRASVVPLGAGRRLALC 68

Query: 70  WWIFTGLTHIVLEGYF-VFSPEFYKDGSYLAEVWKEYSKGDSRYATRDSAIVSLEGLTAV 129
           W+      H+V+EG+F +++    +D ++L+++WKEYSKGDSRY   DS +V +E +TA 
Sbjct: 69  WFAVCTFIHLVIEGWFSLYNGILLEDQAFLSQLWKEYSKGDSRYILSDSFVVCMETVTAC 128

Query: 130 LEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFS--SSPIYYYL 189
           L GP  +  V A    + + +VLQ  +S+GQ+YG  +Y++T L +G        P+Y++ 
Sbjct: 129 LWGPLSLWVVIAFLRQQPFRFVLQLVVSMGQIYGDVLYFLTELHEGLQHGEIGHPVYFWF 188

Query: 190 YYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKV 222
           Y+V LN+ W++IPS++ +   K +  A S    K  K+
Sbjct: 189 YFVFLNAVWLVIPSILVLDAIKHLTSAQSVLDSKVMKI 225

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BLAST of CmoCh14G014230 vs. Swiss-Prot
Match: EBP_CAVPO (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cavia porcellus GN=EBP PE=1 SV=3)

HSP 1 Score: 129.0 bits (323), Expect = 6.3e-29
Identity = 77/205 (37.56%), Postives = 112/205 (54.63%), Query Frame = 1


Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGR---LPKK 60
           MA  S    HPY P  L L  +VP  L    I+ V       + + +W LS R   +P  
Sbjct: 1   MATTSTGPLHPYWPRHLRLDHFVPNDLSAWYIVTVLFTVFGALVVTMWLLSSRASVVPLG 60

Query: 61  TKIERLLMCWWIFTGLTHIVLEGYFV-FSPEFYKDGSYLAEVWKEYSKGDSRYATRDSAI 120
           T   RL +CW+      H+V+EG+FV +      D ++L+++WKEY+KGDSRY   D+ I
Sbjct: 61  TW-RRLSVCWFAVCAFVHLVIEGWFVLYQKAILGDQAFLSQLWKEYAKGDSRYIIEDNFI 120

Query: 121 VSLEGLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFS- 180
           + +E +T VL GP  + AV A        YVLQF ISLGQ+YG  +Y++T   DG     
Sbjct: 121 ICMESITVVLWGPLSLWAVIAFLRQHPSRYVLQFVISLGQIYGDLLYFLTEYRDGFQHGE 180

Query: 181 -SSPIYYYLYYVLLNSFWILIPSLI 200
              PIY++ Y+  +N  W++IP ++
Sbjct: 181 MGHPIYFWFYFFFMNVLWLVIPGVL 204

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BLAST of CmoCh14G014230 vs. TrEMBL
Match: A0A0A0KVP4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G007050 PE=4 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 1.9e-96
Identity = 179/222 (80.63%), Postives = 198/222 (89.19%), Query Frame = 1


Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60
           MAG SEAA HPY PTDL+LP YVPISLPL+TILG Y+L+SLF+ LL+WTLSGRL KK KI
Sbjct: 1   MAGASEAAPHPYVPTDLKLPDYVPISLPLSTILGGYLLTSLFLVLLVWTLSGRLTKKAKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDGS--YLAEVWKEYSKGDSRYATRDSAIVS 120
           +RLLMCWWIFTGLTH+VLEGYFVFSPEFYKD +  YLAEVWKEYSKGDSRYA R+S IVS
Sbjct: 61  DRLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 121 LEGLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSP 180
           +EGLT+VLEGPACILA YAIA+ KSYSY+LQFAISLGQLYGT +YY+TA+LDGD+FSS P
Sbjct: 121 IEGLTSVLEGPACILAAYAIASGKSYSYILQFAISLGQLYGTLLYYVTAVLDGDDFSSGP 180

Query: 181 IYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTK 221
            YYY YYV+ NSFWILIPSLI IRCWKKICIAMS ES  KTK
Sbjct: 181 FYYYAYYVVANSFWILIPSLIGIRCWKKICIAMSIESQTKTK 222

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BLAST of CmoCh14G014230 vs. TrEMBL
Match: A0A0D2RMS0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_006G002800 PE=4 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 2.8e-84
Identity = 158/214 (73.83%), Postives = 181/214 (84.58%), Query Frame = 1


Query: 10  HPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKIERLLMCWWI 69
           HPY P DL+LPGYVP+SL  +TIL VY LSSL +  L+W LSGR    +K++RLLMCWW 
Sbjct: 3   HPYVPRDLQLPGYVPVSLSQSTILTVYGLSSLLVVSLVWFLSGRSRSISKLDRLLMCWWA 62

Query: 70  FTGLTHIVLEGYFVFSPEFYKD--GSYLAEVWKEYSKGDSRYATRDSAIVSLEGLTAVLE 129
           FTGLTHI+LEGYF FSPEFYKD  G YLAEVWKEYSKGDSRYA RDSAIV++EG+T+VLE
Sbjct: 63  FTGLTHIILEGYFAFSPEFYKDKTGFYLAEVWKEYSKGDSRYAGRDSAIVAVEGMTSVLE 122

Query: 130 GPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIYYYLYYVL 189
           GP C+LAVYAIA  K YSY+LQFAISLGQLYGTF+Y+ITA L+GDNFS+SP YYY YYVL
Sbjct: 123 GPPCLLAVYAIAKGKGYSYILQFAISLGQLYGTFVYFITAYLEGDNFSASPFYYYAYYVL 182

Query: 190 LNSFWILIPSLIAIRCWKKICIAMSSESHKKTKV 222
            NSFW+LIPSLIAIRCWKKI  A+ S+S KK K+
Sbjct: 183 ANSFWLLIPSLIAIRCWKKISSAVQSQSQKKNKI 216

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BLAST of CmoCh14G014230 vs. TrEMBL
Match: A0A0B0NQY0_GOSAR (Uncharacterized protein OS=Gossypium arboreum GN=F383_05106 PE=4 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 4.8e-84
Identity = 157/214 (73.36%), Postives = 181/214 (84.58%), Query Frame = 1


Query: 10  HPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKIERLLMCWWI 69
           HPY P DL+LPGYVP+SL  +TIL VY LSSL +  L+W LSGR    +K++RLLMCWW 
Sbjct: 3   HPYVPRDLQLPGYVPVSLSQSTILTVYGLSSLLVVSLVWFLSGRSRNISKLDRLLMCWWA 62

Query: 70  FTGLTHIVLEGYFVFSPEFYKD--GSYLAEVWKEYSKGDSRYATRDSAIVSLEGLTAVLE 129
           FTGLTH++LEGYF FSPEFYKD  G YLAEVWKEYSKGDSRYA RDSAIV++EG+T+VLE
Sbjct: 63  FTGLTHLILEGYFAFSPEFYKDKTGFYLAEVWKEYSKGDSRYAGRDSAIVAVEGMTSVLE 122

Query: 130 GPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIYYYLYYVL 189
           GP C+LAVYAIA  K YSY+LQFAISLGQLYGTF+Y+ITA L+GDNFS+SP YYY YYVL
Sbjct: 123 GPPCLLAVYAIAKGKGYSYILQFAISLGQLYGTFVYFITAYLEGDNFSASPFYYYAYYVL 182

Query: 190 LNSFWILIPSLIAIRCWKKICIAMSSESHKKTKV 222
            NSFW+LIPSLIAIRCWKKI  A+ S+S KK K+
Sbjct: 183 ANSFWLLIPSLIAIRCWKKISSAVQSQSQKKNKI 216

[Close]

BLAST of CmoCh14G014230 vs. TrEMBL
Match: D6PY90_GOSHI (Putative C-8,7 sterol isomerase OS=Gossypium hirsutum GN=HYDRA1 PE=2 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 8.2e-84
Identity = 157/214 (73.36%), Postives = 180/214 (84.11%), Query Frame = 1


Query: 10  HPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKIERLLMCWWI 69
           HPY P DL+LPGYVP+SL  +TIL VY LSSL +  L+W LSGR    +K++RLLMCWW 
Sbjct: 3   HPYVPRDLQLPGYVPVSLSQSTILTVYGLSSLLVVSLVWFLSGRSRSISKLDRLLMCWWA 62

Query: 70  FTGLTHIVLEGYFVFSPEFYKD--GSYLAEVWKEYSKGDSRYATRDSAIVSLEGLTAVLE 129
           FTGLTHI+LEGYF FSPEFYKD  G YLAEVWKEYSKGDSRYA RDS IV++EG+T+VLE
Sbjct: 63  FTGLTHIILEGYFAFSPEFYKDKTGFYLAEVWKEYSKGDSRYAGRDSTIVAVEGITSVLE 122

Query: 130 GPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIYYYLYYVL 189
           GP C+LAVYAIA  K YSY+LQFAISLGQLYGTF+Y+ITA L+GDNFS+SP YYY YYVL
Sbjct: 123 GPPCLLAVYAIAKGKGYSYILQFAISLGQLYGTFVYFITAYLEGDNFSASPFYYYAYYVL 182

Query: 190 LNSFWILIPSLIAIRCWKKICIAMSSESHKKTKV 222
            NSFW+LIPSLIAIRCWKKI  A+ S+S KK K+
Sbjct: 183 ANSFWLLIPSLIAIRCWKKISSAVQSQSQKKNKI 216

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BLAST of CmoCh14G014230 vs. TrEMBL
Match: A0A061DW20_THECC (C-8,7 sterol isomerase isoform 1 OS=Theobroma cacao GN=TCM_003533 PE=4 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 3.4e-82
Identity = 153/214 (71.50%), Postives = 179/214 (83.64%), Query Frame = 1


Query: 10  HPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKIERLLMCWWI 69
           HPY P DL+LPGYV ISL  +TILGV+ L+SLF+  L+W +SGRL K + ++R LMCWW 
Sbjct: 3   HPYVPRDLQLPGYVAISLSQSTILGVFGLASLFVVSLVWIVSGRLRKVSNLDRFLMCWWA 62

Query: 70  FTGLTHIVLEGYFVFSPEFYKD--GSYLAEVWKEYSKGDSRYATRDSAIVSLEGLTAVLE 129
           FTGLTH++LEGYF FSPEFYKD  G YLAEVWKEYSKGDSRYA RD+AIV++EG+TAVLE
Sbjct: 63  FTGLTHMILEGYFAFSPEFYKDKAGFYLAEVWKEYSKGDSRYAGRDAAIVTVEGITAVLE 122

Query: 130 GPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIYYYLYYVL 189
           GP  +LAVYAIA  K YSY LQFAISLGQLYGT +Y+ITA LDGDNF++SP YYY YYVL
Sbjct: 123 GPTSLLAVYAIAKGKGYSYTLQFAISLGQLYGTLVYFITAYLDGDNFAASPFYYYAYYVL 182

Query: 190 LNSFWILIPSLIAIRCWKKICIAMSSESHKKTKV 222
            NSFW+LIPSLIA+RCWKKI +A+  +S KK KV
Sbjct: 183 ANSFWVLIPSLIAVRCWKKISLAVQGQSQKKNKV 216

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BLAST of CmoCh14G014230 vs. TAIR10
Match: AT1G20050.1 (AT1G20050.1 C-8,7 sterol isomerase)

HSP 1 Score: 279.6 bits (714), Expect = 1.6e-75
Identity = 137/224 (61.16%), Postives = 177/224 (79.02%), Query Frame = 1


Query: 6   EAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKIERLLM 65
           E   HPY P DL LPGYVPIS+ +++I+ +Y+ SSL +  L+W L GR  KK K+++LLM
Sbjct: 2   EELAHPYVPRDLNLPGYVPISMSMSSIVSIYLGSSLLVVSLVWLLFGR--KKAKLDKLLM 61

Query: 66  CWWIFTGLTHIVLEGYFVFSPEFYKDGS--YLAEVWKEYSKGDSRYATRDSAIVSLEGLT 125
           CWW FTGLTH++LEGYFVFSPEF+KD +  YLAEVWKEYSKGDSRY  RDSA+VS+EG+T
Sbjct: 62  CWWTFTGLTHVILEGYFVFSPEFFKDNTSAYLAEVWKEYSKGDSRYVGRDSAVVSVEGIT 121

Query: 126 AVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIYYYL 185
           AV+ GPA +LA+YAIA +KSYSYVLQ AIS+ QLYG  +Y+ITA+L+GDNF+++  YYY 
Sbjct: 122 AVIVGPASLLAIYAIAKEKSYSYVLQLAISVCQLYGCLVYFITAILEGDNFATNSFYYYS 181

Query: 186 YYVLLNSFWILIPSLIAIRCWKKICIA-------MSSESHKKTK 221
           YY+  N +W+LIPSLI+ RCWKKIC A       + +++ KKT+
Sbjct: 182 YYIGANCWWVLIPSLISFRCWKKICAAAAIANNNVETKTKKKTR 223

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BLAST of CmoCh14G014230 vs. NCBI nr
Match: gi|659108366|ref|XP_008454161.1| (PREDICTED: probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucumis melo])

HSP 1 Score: 362.5 bits (929), Expect = 5.4e-97
Identity = 182/222 (81.98%), Postives = 199/222 (89.64%), Query Frame = 1


Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60
           MAG SEAA HPY PTDL+LP YVPISLPL+TILG Y+LSSLF+ LLLWTLSGRL KKTKI
Sbjct: 1   MAGASEAAPHPYVPTDLKLPDYVPISLPLSTILGGYLLSSLFLVLLLWTLSGRLTKKTKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDGS--YLAEVWKEYSKGDSRYATRDSAIVS 120
           +RLLMCWWIFTGLTH+VLEGYFVFSPEFYKD +  YLAEVWKEYSKGDSRYA R+S IV 
Sbjct: 61  DRLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVG 120

Query: 121 LEGLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSP 180
           +EGLT+VLEGPACILA YAIA+ KSYSY+LQFAISLGQLYGT +YYITA+LDGD+FSS P
Sbjct: 121 IEGLTSVLEGPACILAAYAIASGKSYSYILQFAISLGQLYGTLLYYITAVLDGDDFSSGP 180

Query: 181 IYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTK 221
           IYYY YYV+ NSFW+LIPSLIAIRCWKKIC AMS ES  KTK
Sbjct: 181 IYYYSYYVVANSFWLLIPSLIAIRCWKKICNAMSIESQMKTK 222

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BLAST of CmoCh14G014230 vs. NCBI nr
Match: gi|449468918|ref|XP_004152168.1| (PREDICTED: probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucumis sativus])

HSP 1 Score: 360.1 bits (923), Expect = 2.7e-96
Identity = 179/222 (80.63%), Postives = 198/222 (89.19%), Query Frame = 1


Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60
           MAG SEAA HPY PTDL+LP YVPISLPL+TILG Y+L+SLF+ LL+WTLSGRL KK KI
Sbjct: 1   MAGASEAAPHPYVPTDLKLPDYVPISLPLSTILGGYLLTSLFLVLLVWTLSGRLTKKAKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDGS--YLAEVWKEYSKGDSRYATRDSAIVS 120
           +RLLMCWWIFTGLTH+VLEGYFVFSPEFYKD +  YLAEVWKEYSKGDSRYA R+S IVS
Sbjct: 61  DRLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 121 LEGLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSP 180
           +EGLT+VLEGPACILA YAIA+ KSYSY+LQFAISLGQLYGT +YY+TA+LDGD+FSS P
Sbjct: 121 IEGLTSVLEGPACILAAYAIASGKSYSYILQFAISLGQLYGTLLYYVTAVLDGDDFSSGP 180

Query: 181 IYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTK 221
            YYY YYV+ NSFWILIPSLI IRCWKKICIAMS ES  KTK
Sbjct: 181 FYYYAYYVVANSFWILIPSLIGIRCWKKICIAMSIESQTKTK 222

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BLAST of CmoCh14G014230 vs. NCBI nr
Match: gi|823166801|ref|XP_012483353.1| (PREDICTED: probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Gossypium raimondii])

HSP 1 Score: 319.7 bits (818), Expect = 4.0e-84
Identity = 158/214 (73.83%), Postives = 181/214 (84.58%), Query Frame = 1


Query: 10  HPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKIERLLMCWWI 69
           HPY P DL+LPGYVP+SL  +TIL VY LSSL +  L+W LSGR    +K++RLLMCWW 
Sbjct: 3   HPYVPRDLQLPGYVPVSLSQSTILTVYGLSSLLVVSLVWFLSGRSRSISKLDRLLMCWWA 62

Query: 70  FTGLTHIVLEGYFVFSPEFYKD--GSYLAEVWKEYSKGDSRYATRDSAIVSLEGLTAVLE 129
           FTGLTHI+LEGYF FSPEFYKD  G YLAEVWKEYSKGDSRYA RDSAIV++EG+T+VLE
Sbjct: 63  FTGLTHIILEGYFAFSPEFYKDKTGFYLAEVWKEYSKGDSRYAGRDSAIVAVEGMTSVLE 122

Query: 130 GPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIYYYLYYVL 189
           GP C+LAVYAIA  K YSY+LQFAISLGQLYGTF+Y+ITA L+GDNFS+SP YYY YYVL
Sbjct: 123 GPPCLLAVYAIAKGKGYSYILQFAISLGQLYGTFVYFITAYLEGDNFSASPFYYYAYYVL 182

Query: 190 LNSFWILIPSLIAIRCWKKICIAMSSESHKKTKV 222
            NSFW+LIPSLIAIRCWKKI  A+ S+S KK K+
Sbjct: 183 ANSFWLLIPSLIAIRCWKKISSAVQSQSQKKNKI 216

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BLAST of CmoCh14G014230 vs. NCBI nr
Match: gi|728834048|gb|KHG13491.1| (hypothetical protein F383_05106 [Gossypium arboreum])

HSP 1 Score: 318.9 bits (816), Expect = 6.9e-84
Identity = 157/214 (73.36%), Postives = 181/214 (84.58%), Query Frame = 1


Query: 10  HPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKIERLLMCWWI 69
           HPY P DL+LPGYVP+SL  +TIL VY LSSL +  L+W LSGR    +K++RLLMCWW 
Sbjct: 3   HPYVPRDLQLPGYVPVSLSQSTILTVYGLSSLLVVSLVWFLSGRSRNISKLDRLLMCWWA 62

Query: 70  FTGLTHIVLEGYFVFSPEFYKD--GSYLAEVWKEYSKGDSRYATRDSAIVSLEGLTAVLE 129
           FTGLTH++LEGYF FSPEFYKD  G YLAEVWKEYSKGDSRYA RDSAIV++EG+T+VLE
Sbjct: 63  FTGLTHLILEGYFAFSPEFYKDKTGFYLAEVWKEYSKGDSRYAGRDSAIVAVEGMTSVLE 122

Query: 130 GPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIYYYLYYVL 189
           GP C+LAVYAIA  K YSY+LQFAISLGQLYGTF+Y+ITA L+GDNFS+SP YYY YYVL
Sbjct: 123 GPPCLLAVYAIAKGKGYSYILQFAISLGQLYGTFVYFITAYLEGDNFSASPFYYYAYYVL 182

Query: 190 LNSFWILIPSLIAIRCWKKICIAMSSESHKKTKV 222
            NSFW+LIPSLIAIRCWKKI  A+ S+S KK K+
Sbjct: 183 ANSFWLLIPSLIAIRCWKKISSAVQSQSQKKNKI 216

[Close]

BLAST of CmoCh14G014230 vs. NCBI nr
Match: gi|295368128|gb|ADG02944.1| (putative C-8,7 sterol isomerase [Gossypium hirsutum])

HSP 1 Score: 318.2 bits (814), Expect = 1.2e-83
Identity = 157/214 (73.36%), Postives = 180/214 (84.11%), Query Frame = 1


Query: 10  HPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKIERLLMCWWI 69
           HPY P DL+LPGYVP+SL  +TIL VY LSSL +  L+W LSGR    +K++RLLMCWW 
Sbjct: 3   HPYVPRDLQLPGYVPVSLSQSTILTVYGLSSLLVVSLVWFLSGRSRSISKLDRLLMCWWA 62

Query: 70  FTGLTHIVLEGYFVFSPEFYKD--GSYLAEVWKEYSKGDSRYATRDSAIVSLEGLTAVLE 129
           FTGLTHI+LEGYF FSPEFYKD  G YLAEVWKEYSKGDSRYA RDS IV++EG+T+VLE
Sbjct: 63  FTGLTHIILEGYFAFSPEFYKDKTGFYLAEVWKEYSKGDSRYAGRDSTIVAVEGITSVLE 122

Query: 130 GPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIYYYLYYVL 189
           GP C+LAVYAIA  K YSY+LQFAISLGQLYGTF+Y+ITA L+GDNFS+SP YYY YYVL
Sbjct: 123 GPPCLLAVYAIAKGKGYSYILQFAISLGQLYGTFVYFITAYLEGDNFSASPFYYYAYYVL 182

Query: 190 LNSFWILIPSLIAIRCWKKICIAMSSESHKKTKV 222
            NSFW+LIPSLIAIRCWKKI  A+ S+S KK K+
Sbjct: 183 ANSFWLLIPSLIAIRCWKKISSAVQSQSQKKNKI 216

The following BLAST results are available for this feature:

BLAST of CmoCh14G014230 vs. Swiss-Prot


Match Name	E-value	Identity	Description
EBP_ARATH	2.9e-74	61.16	Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Arabidopsis thalia...	[more]
EBP_ORYSJ	9.3e-65	57.82	Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Oryza sativa subsp...	[more]
EBP_RAT	1.4e-31	37.44	3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Rattus norvegicus GN=Ebp PE...	[more]
EBP_MOUSE	1.1e-30	36.24	3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Mus musculus GN=Ebp PE=1 SV...	[more]
EBP_CAVPO	6.3e-29	37.56	3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cavia porcellus GN=EBP PE=1...	[more]

BLAST of CmoCh14G014230 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KVP4_CUCSA	1.9e-96	80.63	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G007050 PE=4 SV=1	[more]
A0A0D2RMS0_GOSRA	2.8e-84	73.83	Uncharacterized protein OS=Gossypium raimondii GN=B456_006G002800 PE=4 SV=1	[more]
A0A0B0NQY0_GOSAR	4.8e-84	73.36	Uncharacterized protein OS=Gossypium arboreum GN=F383_05106 PE=4 SV=1	[more]
D6PY90_GOSHI	8.2e-84	73.36	Putative C-8,7 sterol isomerase OS=Gossypium hirsutum GN=HYDRA1 PE=2 SV=1	[more]
A0A061DW20_THECC	3.4e-82	71.50	C-8,7 sterol isomerase isoform 1 OS=Theobroma cacao GN=TCM_003533 PE=4 SV=1	[more]

BLAST of CmoCh14G014230 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G20050.1	1.6e-75	61.16	C-8,7 sterol isomerase	[more]

BLAST of CmoCh14G014230 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659108366\|ref\|XP_008454161.1\|	5.4e-97	81.98	PREDICTED: probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucumis m...	[more]
gi\|449468918\|ref\|XP_004152168.1\|	2.7e-96	80.63	PREDICTED: probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucumis s...	[more]
gi\|823166801\|ref\|XP_012483353.1\|	4.0e-84	73.83	PREDICTED: probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Gossypium...	[more]
gi\|728834048\|gb\|KHG13491.1\|	6.9e-84	73.36	hypothetical protein F383_05106 [Gossypium arboreum]	[more]
gi\|295368128\|gb\|ADG02944.1\|	1.2e-83	73.36	putative C-8,7 sterol isomerase [Gossypium hirsutum]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR007905	EBP

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane

Vocabulary: **Biological Process**
Term	Definition
GO:0016125	sterol metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0047750	cholestenol delta-isomerase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0060964	regulation of gene silencing by miRNA
biological_process	GO:0006694	steroid biosynthetic process
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0000247	C-8 sterol isomerase activity
molecular_function	GO:0047750	cholestenol delta-isomerase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh14G014230.1	CmoCh14G014230.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007905	Emopamil-binding protein	PANTHER	PTHR14207	STEROL ISOMERASE	coord: 1..220 score: 6.4E
IPR007905	Emopamil-binding protein	PFAM	PF05241	EBP	coord: 37..208 score: 3.7
None	No IPR available	PANTHER	PTHR14207:SF0	3-BETA-HYDROXYSTEROID-DELTA(8),DELTA(7)-ISOMERASE	coord: 1..220 score: 6.4E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmoCh14G014230	Cucsa.095880	Cucumber (Gy14) v1	cgycmoB0186
CmoCh14G014230	Cla012759	Watermelon (97103) v1	cmowmB218
CmoCh14G014230	Csa4G007050	Cucumber (Chinese Long) v2	cmocuB245
CmoCh14G014230	MELO3C017829	Melon (DHL92) v3.5.1	cmomeB232
CmoCh14G014230	ClCG07G012080	Watermelon (Charleston Gray)	cmowcgB230
CmoCh14G014230	CSPI04G02120	Wild cucumber (PI 183967)	cmocpiB250
CmoCh14G014230	Lsi07G004030	Bottle gourd (USVL1VR-Ls)	cmolsiB244
CmoCh14G014230	Cp4.1LG03g13440	Cucurbita pepo (Zucchini)	cmocpeB245
CmoCh14G014230	MELO3C017829.2	Melon (DHL92) v3.6.1	cmomedB264
CmoCh14G014230	CsaV3_4G002070	Cucumber (Chinese Long) v3	cmocucB0291
CmoCh14G014230	Cla97C07G138820	Watermelon (97103) v2	cmowmbB255
CmoCh14G014230	Bhi01G002827	Wax gourd	cmowgoB0306
CmoCh14G014230	CsGy4G002050	Cucumber (Gy14) v2	cgybcmoB455
CmoCh14G014230	Carg06539	Silver-seed gourd	carcmoB0163

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh14G014230	Cucurbita maxima (Rimu)	cmacmoB245