Carg27609 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg27609
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Glycoprotein membrane GPI-anchored, putative
Location	Cucurbita_argyrosperma_scaffold_314 : 62174 .. 64814 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TATGTTCTTCGCCTCTTTTTATTGCTTTGGGTTCGCAGTTCATTTGTGAATTGTTTCCCACAAATATGAGAGTGTTTGCTTTGTTCTTCTTTGCTGTCATCGCCACCTTCCTCCTTCTGCCCAATGCTGTTCTTTGTGACGGTATGATTTTCATCGTTATTTTTCTTTTAACGTTTGTAGCTATTCATCGAAGAATATTGCATGTTGTAATCGCTTATTTTGATATGACGTTTATGTATCATTCAATTTGATAGCTTAAACAGATAAGATTAAAGGTGTTTACGGGATAGGAGGGGTAGGAACGGTAATCCTTCTTCATCCTCGTTCTTCGAAGTTTCTCATTTATTTTCGTATGGATTGAATTTATCCCGAAGAATTTTTTTCCCTTTTTATATATTTTTTCAAAAATATATACCATACAAAAAAATTTAATAATATTTTAGAAATCAAAATATAATATAATTTCTAAGAACTCAAATTTCAATATTACAATTACTTATTCCACACTTCATTTAATGGACGGGGCAAAGAATAGTATCCATGTTTCGAGTCCTATTTAGCTACTTGAGATATATATCTCCACTCCCTTTCTCATTCTCAGTTTAAAAGTAGTTTTTGAAATAGTGTAAAAAGAGGTGCTTCGAATCATTACTATTTGACTCGAACATGTTATAAAAAAGTCGAGGCATTTCGAATTGTTTGTTGACACCGACAAAGTCAAGGAAATGTGTAAAATTATTTCAATATTGGACCTTGGACATATAATAGTTCCGGATCGAATATCTTTAGGATCTTTGGTAGATTTGGTGAGATCTCACATCAGTTGAAGAGGGAACGAAACATTCTTTGTAAGTGTATGAAAATCTCTCTCTATCAAACACGTTTTAAAAACCTTGGGAGAAGTCCGAAAGAGAAAGCCCAAAGAGGACAATATCGGTTAACGGTGGGCTTGGGTTGGGGCGTTACAAATGGTACCAGAACCAAATAACGAACGGTGAGCAAGAAAGGACGCTGAGCCCCAAAAGGAGTGAACATCGGGTAGTGTCCCAGCAAGGAAGTTGGGCCCTAACAATGTGCTAGGGAGAACATTGGGCCTCGAGGGGGGAACTAAGCATTCTTTATAAGGGTGTGGAAACCTCTCTCTAGCATACGTGTTTTAAAAACCTTGATGGGTAGTCTCCCAAAGAAAACAATATCGGCTAGCGGTGAGCCCTAACAGTGTGTTAGGGAGAACATTGGGCCTCGAGAGGGGAACTAAGCATTCTTTATAAGGGTGTGGAAACCTCTCTCTAGCATACGTGTTTTAAAAACCTTGATGGGTAGTCTCCCAAAGAAAACAATATCGACTAGCGGTGAGTCCTAACAGTGTGTTAGGGAGAACATTGGGCCTCGAGAGGGGAACTAAGCATTCTTTATAAGGGTGTGGGAAACCTCTCTCTAGCGTACGTGTTTTTAAAAACCTCGATGGGTAAAAACAATATCGGCGAGTGTGTTAGGGTGAACATTGGGCCTCGAGAGGGGAACTAAGCATTCTTTATAAGGGTGTGGAAACCTCTCTCTAGCATACGTGTTTTAAAAACCTTGATGGGTAGTCTCCCAAAGAAAACAATATCGACTAGCGGTGAGTCCTAACAGTGTGTTAGGGAGAACATTGGGCCTCGAGAGGGGAACTAAGTATTTTTTATAAGGGTGTGGAAACCTCTCTCTAGCGTACGTGTTTTAAAAACTTCGATGGGAAGTCTCCCAGAGAAAAACAATATCCGCTAGCGGTGGATTTAGGCCGTTCGAGGTAGATCTCGACAAATAAATTATAGCGAATTTAATTAGTGTTTCATATTGTTAATGAAGTTACATGCACACCGCACTAAACATAACCAATGCCTCACATGGCTTAGTTTTCTTGTGCAGTGAAAGATACAGAGATTCTAAACGCCATCAACAATTACAGACAATCAAAGCACCTATCTAATCTTACTCATAACAAAAATGCAGCATGTTTAGCAAGCAGGTTCGTTTATAAGCTAAGAGATGAGCCTTGTTCAAGTCCAGATGATTTCCACAAAGAAATCAACTCTGAAAATACCCTTGCAGATTTTCTTAAGCTCTTGAAGAAGTGTCACATAGCCTACAACTCGACCGTTGATGGCATCATGCTCCCCGCTTGCGTCCCCCACCTCGATCCCAAGTCCGTCGCCGATAACTATACCAAATCCCATGACGTCGATTATATTAACAGTAAGAACTTTACGGGTGCTGGGGTCGGGACGTCTGACGATTGGATCGTCCTCGTTCTTAGCACCAATACGTCGACGGGGAACTTTGCTAACGGTGGATCTTCGTCTTTGGTTGTCGCCCGGGGTGGTTGCATTGGTATCATGGTTGGTTTCTTAGGGTTGTTTGTTCTTTTGTTATTTTGATGAGGTGAGGGAAAAGGCTTTTGGGTTTGGGTTTAAAGCATTGGTAACCTAAATAACACAATTGTTTTACAACTCGATCCTACTTAACCCTATCGGGTTGGATTGAATTAGGTTGTTTTTAAAAATAAATTATACCCACGATATATAATCATTCGGGACAATTTTAACGAAAATATATCACAAACCCTAAAAAAAATCACAATTTGTAAGGATTTCAAACAAGG

mRNA sequence

TATGTTCTTCGCCTCTTTTTATTGCTTTGGGTTCGCAGTTCATTTGTGAATTGTTTCCCACAAATATGAGAGTGTTTGCTTTGTTCTTCTTTGCTGTCATCGCCACCTTCCTCCTTCTGCCCAATGCTGTTCTTTGTGACGTGAAAGATACAGAGATTCTAAACGCCATCAACAATTACAGACAATCAAAGCACCTATCTAATCTTACTCATAACAAAAATGCAGCATGTTTAGCAAGCAGGTTCGTTTATAAGCTAAGAGATGAGCCTTGTTCAAGTCCAGATGATTTCCACAAAGAAATCAACTCTGAAAATACCCTTGCAGATTTTCTTAAGCTCTTGAAGAAGTGTCACATAGCCTACAACTCGACCGTTGATGGCATCATGCTCCCCGCTTGCGTCCCCCACCTCGATCCCAAGTCCGTCGCCGATAACTATACCAAATCCCATGACGTCGATTATATTAACAGTAAGAACTTTACGGGTGCTGGGGTCGGGACGTCTGACGATTGGATCGTCCTCGTTCTTAGCACCAATACGTCGACGGGGAACTTTGCTAACGGTGGATCTTCGTCTTTGGTTGTCGCCCGGGGTGGTTGCATTGGTATCATGGTTGGTTTCTTAGGGTTGTTTGTTCTTTTGTTATTTTGATGAGGTGAGGGAAAAGGCTTTTGGGTTTGGGTTTAAAGCATTGGTAACCTAAATAACACAATTGTTTTACAACTCGATCCTACTTAACCCTATCGGGTTGGATTGAATTAGGTTGTTTTTAAAAATAAATTATACCCACGATATATAATCATTCGGGACAATTTTAACGAAAATATATCACAAACCCTAAAAAAAATCACAATTTGTAAGGATTTCAAACAAGG

Coding sequence (CDS)

ATGAGAGTGTTTGCTTTGTTCTTCTTTGCTGTCATCGCCACCTTCCTCCTTCTGCCCAATGCTGTTCTTTGTGACGTGAAAGATACAGAGATTCTAAACGCCATCAACAATTACAGACAATCAAAGCACCTATCTAATCTTACTCATAACAAAAATGCAGCATGTTTAGCAAGCAGGTTCGTTTATAAGCTAAGAGATGAGCCTTGTTCAAGTCCAGATGATTTCCACAAAGAAATCAACTCTGAAAATACCCTTGCAGATTTTCTTAAGCTCTTGAAGAAGTGTCACATAGCCTACAACTCGACCGTTGATGGCATCATGCTCCCCGCTTGCGTCCCCCACCTCGATCCCAAGTCCGTCGCCGATAACTATACCAAATCCCATGACGTCGATTATATTAACAGTAAGAACTTTACGGGTGCTGGGGTCGGGACGTCTGACGATTGGATCGTCCTCGTTCTTAGCACCAATACGTCGACGGGGAACTTTGCTAACGGTGGATCTTCGTCTTTGGTTGTCGCCCGGGGTGGTTGCATTGGTATCATGGTTGGTTTCTTAGGGTTGTTTGTTCTTTTGTTATTTTGA

Protein sequence

MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRFVYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSVADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIGIMVGFLGLFVLLLF

Homology

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BLAST of Carg27609 vs. NCBI nr
Match: XP_022961524.1 (uncharacterized GPI-anchored protein At5g19250-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 382.9 bits (982), Expect = 6.6e-103
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF 60
           MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF
Sbjct: 32  MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF 91

Query: 61  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 120
           VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV
Sbjct: 92  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 151

Query: 121 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 180
           ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG
Sbjct: 152 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 211

Query: 181 IMVGFLGLFVLLLF 195
           IMVGFLGLFVLLLF
Sbjct: 212 IMVGFLGLFVLLLF 225

[Close]

BLAST of Carg27609 vs. NCBI nr
Match: XP_023516540.1 (uncharacterized GPI-anchored protein At5g19250-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 370.9 bits (951), Expect = 2.6e-99
Identity = 187/194 (96.39%), Postives = 191/194 (98.45%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF 60
           MRVFALFFF V+ATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF
Sbjct: 1   MRVFALFFFVVVATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF 60

Query: 61  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 120
           VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV
Sbjct: 61  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 120

Query: 121 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 180
           + NYTKS DV+YI+SKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG
Sbjct: 121 SSNYTKSRDVEYISSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 180

Query: 181 IMVGFLGLFVLLLF 195
           IMVGFLGLFVLLLF
Sbjct: 181 IMVGFLGLFVLLLF 194

[Close]

BLAST of Carg27609 vs. NCBI nr
Match: XP_022968795.1 (uncharacterized GPI-anchored protein At3g06035-like [Cucurbita maxima])

HSP 1 Score: 349.0 bits (894), Expect = 1.1e-92
Identity = 179/194 (92.27%), Postives = 186/194 (95.88%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF 60
           MRVFAL FFAVIATFLLLPNAVLCDV+D EILNAINNYRQ K LSN T+NKNAACLASRF
Sbjct: 1   MRVFAL-FFAVIATFLLLPNAVLCDVRDIEILNAINNYRQKKSLSNFTYNKNAACLASRF 60

Query: 61  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 120
           VYKLRDEPCSSPDDF+KEINSENTLADF KLLKKCHIAYNSTVDGIMLPACVPHLDP+SV
Sbjct: 61  VYKLRDEPCSSPDDFNKEINSENTLADFHKLLKKCHIAYNSTVDGIMLPACVPHLDPESV 120

Query: 121 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 180
           ++NYTKS DVDYINSKNFTGAGVGTSDDWIVL+LSTNTSTGNFANGGSSSLVVARGGCIG
Sbjct: 121 SNNYTKSRDVDYINSKNFTGAGVGTSDDWIVLILSTNTSTGNFANGGSSSLVVARGGCIG 180

Query: 181 IMVGFLGLFVLLLF 195
           IM GFLGLFVLLLF
Sbjct: 181 IMAGFLGLFVLLLF 193

[Close]

BLAST of Carg27609 vs. NCBI nr
Match: XP_022961525.1 (uncharacterized GPI-anchored protein At3g06035-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 313.2 bits (801), Expect = 6.4e-82
Identity = 166/194 (85.57%), Postives = 166/194 (85.57%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF 60
           MRVFALFFFAVIATFLLLPNAVLCD                            ACLASRF
Sbjct: 32  MRVFALFFFAVIATFLLLPNAVLCD----------------------------ACLASRF 91

Query: 61  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 120
           VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV
Sbjct: 92  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 151

Query: 121 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 180
           ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG
Sbjct: 152 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 197

Query: 181 IMVGFLGLFVLLLF 195
           IMVGFLGLFVLLLF
Sbjct: 212 IMVGFLGLFVLLLF 197

[Close]

BLAST of Carg27609 vs. NCBI nr
Match: XP_008450653.1 (PREDICTED: uncharacterized GPI-anchored protein At5g19250-like [Cucumis melo])

HSP 1 Score: 264.6 bits (675), Expect = 2.6e-67
Identity = 135/196 (68.88%), Postives = 162/196 (82.65%), Query Frame = 0


Query: 2   RVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRFV 61
           R+F LFFF VI+ FLL PN VLCDVKD  IL+AIN YRQSK+LS  ++NKNAACLA + V
Sbjct: 7   RLFTLFFFVVISIFLLHPNVVLCDVKDDNILSAINKYRQSKNLSEFSYNKNAACLAGKLV 66

Query: 62  YKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSVA 121
           YKLRDEPCSS ++F+KEI+SE  LADF KLL+KCHIAYNS++DGI+LP+CVP LDP SV 
Sbjct: 67  YKLRDEPCSSAENFNKEISSETKLADFPKLLRKCHIAYNSSIDGIILPSCVPGLDPTSVT 126

Query: 122 DNYTKSHDVDYINSKNFTGAGVGTSDD-WIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 181
            NYT SHD +YIN +N+TGAGVGT +D W+VL+LSTNTSTGN+ + GSSSLVVA GG +G
Sbjct: 127 SNYTHSHDAEYINDQNYTGAGVGTIEDAWVVLILSTNTSTGNYDSSGSSSLVVAGGGRVG 186

Query: 182 IMVGFLGLFV--LLLF 195
           +MV  LG+FV  LL F
Sbjct: 187 VMVALLGMFVSSLLFF 202

[Close]

BLAST of Carg27609 vs. TAIR10
Match: AT5G19250.1 (Glycoprotein membrane precursor GPI-anchored)

HSP 1 Score: 107.8 bits (268), Expect = 7.4e-24
Identity = 69/197 (35.03%), Postives = 104/197 (52.79%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTE--ILNAINNYRQSKHLSNLTHNKNAACLAS 60
           M +  L    +++ FL L   VL D  D E  +L  IN+YR S +L+ L HN NA CLA 
Sbjct: 1   MAISKLHLLFLLSVFLSLHPLVLSDTADEEDVLLTGINSYRASLNLTTLIHNHNAECLAD 60

Query: 61  RFVYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPK 120
               + +++PC++         +     +   LL KC +    T DG +LPACVP+LDP 
Sbjct: 61  EIADQFKNQPCTNTTGSASVPGTTPGFPNLPNLLSKCRLNPTVTRDGAILPACVPNLDPS 120

Query: 121 SVADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSS-SLVVARGG 180
            V  N+T S     +N   FTG G+G+ D+WIV+VL+T+T  G+++   +S +      G
Sbjct: 121 LVLTNFTMSQYSKDLNDSKFTGIGIGSDDNWIVVVLTTSTPEGSYSPASNSGAFAFGVNG 180

Query: 181 CIGIMVGFLGLFVLLLF 195
            +   + FL LF   +F
Sbjct: 181 LVSSSLMFL-LFCFFMF 196

[Close]

BLAST of Carg27609 vs. TAIR10
Match: AT3G06035.1 (Glycoprotein membrane precursor GPI-anchored)

HSP 1 Score: 107.5 bits (267), Expect = 9.7e-24
Identity = 67/196 (34.18%), Postives = 110/196 (56.12%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTEI-LNAINNYRQSKHLSNLTHNKNAACLASR 60
           M +  L     ++  L L   VL D  + +I L  IN+YR +++L+ L+ N+NA CLA  
Sbjct: 1   MAIDKLPLLLFLSILLCLNRPVLSDTDEEDILLTGINSYRTTQNLTILSKNENAECLADE 60

Query: 61  FVYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKS 120
              + +++PC++         +E   A++ ++L KCH+  + T DG ++PACVP L+   
Sbjct: 61  IADQFKNKPCTNDTGSATVPGTEPQFANYPQILAKCHLNVSDTRDGSIMPACVPRLESNL 120

Query: 121 VADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCI 180
           V  N+TKS     +N   FTG G+G  DDWIV+VL+TNT  G+++   + +   + G   
Sbjct: 121 VLTNFTKSQYSMSLNDSKFTGIGIGKEDDWIVVVLTTNTPEGSYST-ATPTKQESNGFTF 180

Query: 181 GI-MVGFLGLFVLLLF 195
           GI +V +L +F+   F
Sbjct: 181 GIGLVSYLVIFMYSSF 195

[Close]

BLAST of Carg27609 vs. TAIR10
Match: AT1G54860.1 (Glycoprotein membrane precursor GPI-anchored)

HSP 1 Score: 97.1 bits (240), Expect = 1.3e-20
Identity = 58/194 (29.90%), Postives = 102/194 (52.58%), Query Frame = 0


Query: 7   FFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRFVYKLRD 66
           F+   +  F L+   V+ +  +  +L  +N+YR ++ +     N+ A C+A     KL D
Sbjct: 11  FYIIHLLIFSLISTCVVSNQAEDNLLQGLNSYRTAQRVPPFAKNEKADCVADEIADKLED 70

Query: 67  EPCSSPDDFHKEINSEN------TLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 126
           +PC++    H   ++         L ++  +L +C I  N+T DG++LP C+P+  P   
Sbjct: 71  QPCTN----HTTASTVTPGSVPPRLTNYQDILSECKIDPNTTRDGLILPVCIPNRIPTLA 130

Query: 127 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANG-----GSSSLVVAR 186
             NYT++    Y+N   + GAGVG+  +W+V+VL+T+T  G+F  G      +S  V+A 
Sbjct: 131 LTNYTQTGYARYLNDSRYVGAGVGSEKEWMVVVLTTSTPGGSFTAGVAAGKATSVRVMAG 190

Query: 187 GGCIGIMVGFLGLF 190
            G +G++   L LF
Sbjct: 191 LGLMGLLFSCLVLF 200

[Close]

BLAST of Carg27609 vs. TAIR10
Match: AT5G19240.1 (Glycoprotein membrane precursor GPI-anchored)

HSP 1 Score: 96.3 bits (238), Expect = 2.2e-20
Identity = 61/165 (36.97%), Postives = 91/165 (55.15%), Query Frame = 0


Query: 11  VIATFLLLPNAVLCDVKDTEIL-NAINNYRQSKHLSNLTHNKNAACLASRFVYKLRDEPC 70
           +++ FL L   VL +     +L    N YR+S +L+NL  NKNA CLA   V +L+++PC
Sbjct: 12  LLSVFLSLHRPVLSNTDGENLLFTVFNQYRESVNLTNLKKNKNAECLADEVVDQLQNQPC 71

Query: 71  SSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVP--HLDP--KSVADNYT 130
           ++         ++  + +F  LL KC +  N T DG++L  C P  H  P   S A+  T
Sbjct: 72  TNTTGSASVPGTDPGIPNFPNLLAKCRLNTNVTRDGLILQVCAPKHHSTPDLSSFANVLT 131

Query: 131 KSHDVDYINSKNFTGAGVGTSDD--WIVLVLSTNTSTGNFANGGS 169
           K+     +N   FTGAGVG   D  W+V VL+TNT  G+++N G+
Sbjct: 132 KN-----LNDSKFTGAGVGIDSDGIWLVTVLTTNTPGGSYSNSGA 171

[Close]

BLAST of Carg27609 vs. TAIR10
Match: AT5G19230.1 (Glycoprotein membrane precursor GPI-anchored)

HSP 1 Score: 76.6 bits (187), Expect = 1.8e-14
Identity = 62/198 (31.31%), Postives = 92/198 (46.46%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVL--CDVKDTEILNAINNYRQSKHLSNLTHNKNAACLAS 60
           M +  L    +++ FL L   VL   D +D  +L   N YR   +L  LT N+NA CLA 
Sbjct: 1   MAISKLHLLCLLSVFLSLHRLVLSGTDGEDDLLLTVFNKYRTGLNLKTLTKNENAECLAD 60

Query: 61  RFVYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPK 120
             V +L+++PC++ +      NS     +   LL KC +      DG+++  C P  D  
Sbjct: 61  EVVDQLKNQPCTNTN------NSAPVPGN--NLLAKCSLNTTVVRDGVIMQVCFPKHDKN 120

Query: 121 SVADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGC 180
               N+  S  +  +N    TG G+G+ D W+V++L+TNT       GG S L     G 
Sbjct: 121 PDLSNF-NSVVLKNLNDSKITGIGIGSGDIWVVVILTTNT-----PEGGYSLLTTTNSGA 180

Query: 181 IGIMVGFL--GLFVLLLF 195
               V  L    F+ LLF
Sbjct: 181 YAFGVNGLVSSSFLFLLF 184

[Close]

BLAST of Carg27609 vs. Swiss-Prot
Match: sp|P59833|UGPI3_ARATH (Uncharacterized GPI-anchored protein At5g19250 OS=Arabidopsis thaliana OX=3702 GN=At5g19250 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.3e-22
Identity = 69/197 (35.03%), Postives = 104/197 (52.79%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTE--ILNAINNYRQSKHLSNLTHNKNAACLAS 60
           M +  L    +++ FL L   VL D  D E  +L  IN+YR S +L+ L HN NA CLA 
Sbjct: 1   MAISKLHLLFLLSVFLSLHPLVLSDTADEEDVLLTGINSYRASLNLTTLIHNHNAECLAD 60

Query: 61  RFVYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPK 120
               + +++PC++         +     +   LL KC +    T DG +LPACVP+LDP 
Sbjct: 61  EIADQFKNQPCTNTTGSASVPGTTPGFPNLPNLLSKCRLNPTVTRDGAILPACVPNLDPS 120

Query: 121 SVADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSS-SLVVARGG 180
            V  N+T S     +N   FTG G+G+ D+WIV+VL+T+T  G+++   +S +      G
Sbjct: 121 LVLTNFTMSQYSKDLNDSKFTGIGIGSDDNWIVVVLTTSTPEGSYSPASNSGAFAFGVNG 180

Query: 181 CIGIMVGFLGLFVLLLF 195
            +   + FL LF   +F
Sbjct: 181 LVSSSLMFL-LFCFFMF 196

[Close]

BLAST of Carg27609 vs. Swiss-Prot
Match: sp|Q84MC0|UGPI4_ARATH (Uncharacterized GPI-anchored protein At3g06035 OS=Arabidopsis thaliana OX=3702 GN=At3g06035 PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.7e-22
Identity = 67/196 (34.18%), Postives = 110/196 (56.12%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTEI-LNAINNYRQSKHLSNLTHNKNAACLASR 60
           M +  L     ++  L L   VL D  + +I L  IN+YR +++L+ L+ N+NA CLA  
Sbjct: 1   MAIDKLPLLLFLSILLCLNRPVLSDTDEEDILLTGINSYRTTQNLTILSKNENAECLADE 60

Query: 61  FVYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKS 120
              + +++PC++         +E   A++ ++L KCH+  + T DG ++PACVP L+   
Sbjct: 61  IADQFKNKPCTNDTGSATVPGTEPQFANYPQILAKCHLNVSDTRDGSIMPACVPRLESNL 120

Query: 121 VADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCI 180
           V  N+TKS     +N   FTG G+G  DDWIV+VL+TNT  G+++   + +   + G   
Sbjct: 121 VLTNFTKSQYSMSLNDSKFTGIGIGKEDDWIVVVLTTNTPEGSYST-ATPTKQESNGFTF 180

Query: 181 GI-MVGFLGLFVLLLF 195
           GI +V +L +F+   F
Sbjct: 181 GIGLVSYLVIFMYSSF 195

[Close]

BLAST of Carg27609 vs. Swiss-Prot
Match: sp|Q84VZ5|UGPI2_ARATH (Uncharacterized GPI-anchored protein At5g19240 OS=Arabidopsis thaliana OX=3702 GN=At5g19240 PE=2 SV=2)

HSP 1 Score: 96.3 bits (238), Expect = 4.0e-19
Identity = 61/165 (36.97%), Postives = 91/165 (55.15%), Query Frame = 0


Query: 11  VIATFLLLPNAVLCDVKDTEIL-NAINNYRQSKHLSNLTHNKNAACLASRFVYKLRDEPC 70
           +++ FL L   VL +     +L    N YR+S +L+NL  NKNA CLA   V +L+++PC
Sbjct: 12  LLSVFLSLHRPVLSNTDGENLLFTVFNQYRESVNLTNLKKNKNAECLADEVVDQLQNQPC 71

Query: 71  SSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVP--HLDP--KSVADNYT 130
           ++         ++  + +F  LL KC +  N T DG++L  C P  H  P   S A+  T
Sbjct: 72  TNTTGSASVPGTDPGIPNFPNLLAKCRLNTNVTRDGLILQVCAPKHHSTPDLSSFANVLT 131

Query: 131 KSHDVDYINSKNFTGAGVGTSDD--WIVLVLSTNTSTGNFANGGS 169
           K+     +N   FTGAGVG   D  W+V VL+TNT  G+++N G+
Sbjct: 132 KN-----LNDSKFTGAGVGIDSDGIWLVTVLTTNTPGGSYSNSGA 171

[Close]

BLAST of Carg27609 vs. Swiss-Prot
Match: sp|Q8GUL8|UGPI1_ARATH (Uncharacterized GPI-anchored protein At5g19230 OS=Arabidopsis thaliana OX=3702 GN=At5g19230 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.3e-13
Identity = 62/198 (31.31%), Postives = 92/198 (46.46%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVL--CDVKDTEILNAINNYRQSKHLSNLTHNKNAACLAS 60
           M +  L    +++ FL L   VL   D +D  +L   N YR   +L  LT N+NA CLA 
Sbjct: 1   MAISKLHLLCLLSVFLSLHRLVLSGTDGEDDLLLTVFNKYRTGLNLKTLTKNENAECLAD 60

Query: 61  RFVYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPK 120
             V +L+++PC++ +      NS     +   LL KC +      DG+++  C P  D  
Sbjct: 61  EVVDQLKNQPCTNTN------NSAPVPGN--NLLAKCSLNTTVVRDGVIMQVCFPKHDKN 120

Query: 121 SVADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGC 180
               N+  S  +  +N    TG G+G+ D W+V++L+TNT       GG S L     G 
Sbjct: 121 PDLSNF-NSVVLKNLNDSKITGIGIGSGDIWVVVILTTNT-----PEGGYSLLTTTNSGA 180

Query: 181 IGIMVGFL--GLFVLLLF 195
               V  L    F+ LLF
Sbjct: 181 YAFGVNGLVSSSFLFLLF 184

[Close]

BLAST of Carg27609 vs. TrEMBL
Match: tr|A0A1S3BQD0|A0A1S3BQD0_CUCME (uncharacterized GPI-anchored protein At5g19250-like OS=Cucumis melo OX=3656 GN=LOC103492177 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.7e-67
Identity = 135/196 (68.88%), Postives = 162/196 (82.65%), Query Frame = 0


Query: 2   RVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRFV 61
           R+F LFFF VI+ FLL PN VLCDVKD  IL+AIN YRQSK+LS  ++NKNAACLA + V
Sbjct: 7   RLFTLFFFVVISIFLLHPNVVLCDVKDDNILSAINKYRQSKNLSEFSYNKNAACLAGKLV 66

Query: 62  YKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSVA 121
           YKLRDEPCSS ++F+KEI+SE  LADF KLL+KCHIAYNS++DGI+LP+CVP LDP SV 
Sbjct: 67  YKLRDEPCSSAENFNKEISSETKLADFPKLLRKCHIAYNSSIDGIILPSCVPGLDPTSVT 126

Query: 122 DNYTKSHDVDYINSKNFTGAGVGTSDD-WIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 181
            NYT SHD +YIN +N+TGAGVGT +D W+VL+LSTNTSTGN+ + GSSSLVVA GG +G
Sbjct: 127 SNYTHSHDAEYINDQNYTGAGVGTIEDAWVVLILSTNTSTGNYDSSGSSSLVVAGGGRVG 186

Query: 182 IMVGFLGLFV--LLLF 195
           +MV  LG+FV  LL F
Sbjct: 187 VMVALLGMFVSSLLFF 202

[Close]

BLAST of Carg27609 vs. TrEMBL
Match: tr|A0A2I4GCP3|A0A2I4GCP3_9ROSI (uncharacterized GPI-anchored protein At3g06035-like OS=Juglans regia OX=51240 GN=LOC109006764 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.1e-32
Identity = 72/159 (45.28%), Postives = 97/159 (61.01%), Query Frame = 0


Query: 5   ALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRFVYKL 64
           +L    +I   + L + VLCD +D  +   IN+YRQS +L   T N NA CLA     +L
Sbjct: 9   SLLLLVLIHGVIFLSDPVLCDDEDDTLFQGINSYRQSLNLPEFTENDNAGCLADEIADQL 68

Query: 65  RDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSVADNY 124
            D PC +  D+     +    ++F KLL  CHI  N+TVDG+++P CVP LDP  V  NY
Sbjct: 69  EDLPCDNASDYSFAPGTTPKFSNFNKLLHSCHINVNNTVDGVLMPVCVPKLDPTLVLSNY 128

Query: 125 TKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNF 164
           T S    Y+N  ++TGAG+ + DDWIV++LSTNTSTGNF
Sbjct: 129 TNSQYAKYLNDSSYTGAGIASEDDWIVVILSTNTSTGNF 167

[Close]

BLAST of Carg27609 vs. TrEMBL
Match: tr|M5W0Y4|M5W0Y4_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G327200 PE=4 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 5.8e-31
Identity = 81/189 (42.86%), Postives = 113/189 (59.79%), Query Frame = 0


Query: 5   ALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRFVYKL 64
           +LF  A+I   L L +   C+ ++ +I   +N+++QS     L  N NA CLA     +L
Sbjct: 7   SLFLLALINCLLFLSHPASCNEEEDKIFQGLNSFKQS-----LKKNSNAECLADEIADEL 66

Query: 65  RDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSVADNY 124
            D+PCSS DD+  E  +      F KL+KKCHI  N+T DGI+LP CVP+ DP  V  NY
Sbjct: 67  EDQPCSSADDYAIEPGNGPKFPKFDKLIKKCHIDINTTTDGIILPVCVPNWDPDLVIKNY 126

Query: 125 TKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIGIMVG 184
           T +    Y+N   +TGAGVGT DDW+V+VLST+T +G+F+   S   + A G    +M  
Sbjct: 127 THTQYAKYLNGSKYTGAGVGTEDDWVVVVLSTDTESGSFSGAPS---LAAIGMVHYMMAM 186

Query: 185 FLGLFVLLL 194
            LGLF++LL
Sbjct: 187 LLGLFLILL 187

[Close]

BLAST of Carg27609 vs. TrEMBL
Match: tr|A0A2I4GSI9|A0A2I4GSI9_9ROSI (uncharacterized GPI-anchored protein At5g19250-like OS=Juglans regia OX=51240 GN=LOC109010467 PE=4 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.9e-30
Identity = 75/188 (39.89%), Postives = 120/188 (63.83%), Query Frame = 0


Query: 6   LFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRFVYKLR 65
           LFF  ++++ LLL + V CD +D  +L  IN+YR S +L+ L HN NA CLA     K +
Sbjct: 11  LFFPLLLSSILLLNHPVKCDDEDDTLLQGINSYRASLNLTALPHNDNAECLADEIADKFK 70

Query: 66  DEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSVADNYT 125
           ++PC++    +    +E   +D+  LL KCH+ +++T DG ++PACVP+L+P  V  N+T
Sbjct: 71  NQPCTNTTGSNTIPGTEPQFSDYPNLLAKCHLNFSNTQDGAIMPACVPNLEPNLVLSNFT 130

Query: 126 KSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIGIMVGF 185
           KS     +N   +TGAG+G+  +WIV+VL+TNTSTG+F    +++ +V+R   I  ++  
Sbjct: 131 KSQYSGNLNDTKYTGAGIGSEKNWIVVVLTTNTSTGSFVPSTNAASLVSRVDLIHHLLFL 190

Query: 186 LGLFVLLL 194
           L + +LLL
Sbjct: 191 LMVSILLL 198

[Close]

BLAST of Carg27609 vs. TrEMBL
Match: tr|A0A2P4MBH0|A0A2P4MBH0_QUESU (Putative gpi-anchored protein OS=Quercus suber OX=58331 GN=CFP56_58344 PE=4 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.9e-29
Identity = 71/174 (40.80%), Postives = 103/174 (59.20%), Query Frame = 0


Query: 17  LLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRFVYKLRDEPCSSPDDFH 76
           +L NA+     ++ +L  +N YR S HL+N T N NAACLA++   +L++E C +   F 
Sbjct: 1   MLTNAIYTSTAESYLLEDLNGYRMSLHLNNFTENSNAACLANKIADQLQNETCKNAFHFS 60

Query: 77  KEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSVADNYTKSHDVDYINSK 136
               ++  +  F K L  C I  N+TVDG++LP CVP LDP  V  NYTKS    Y+   
Sbjct: 61  STPGTKPKVPKFDKFLDNCDINDNNTVDGVILPVCVPRLDPFLVFSNYTKSQYAKYLKDS 120

Query: 137 NFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGG-CIGIMVGFLGLF 190
           N+TG G+G+ D W+V++LSTNTSTG+F+   S    +  G   + + +G L LF
Sbjct: 121 NYTGVGIGSEDIWMVVILSTNTSTGSFSGAASLIANITMGNYTLALFLGLLLLF 174

The following BLAST results are available for this feature:

BLAST of Carg27609 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022961524.1	6.6e-103	100.00	uncharacterized GPI-anchored protein At5g19250-like isoform X1 [Cucurbita moscha...	[more]
XP_023516540.1	2.6e-99	96.39	uncharacterized GPI-anchored protein At5g19250-like [Cucurbita pepo subsp. pepo]	[more]
XP_022968795.1	1.1e-92	92.27	uncharacterized GPI-anchored protein At3g06035-like [Cucurbita maxima]	[more]
XP_022961525.1	6.4e-82	85.57	uncharacterized GPI-anchored protein At3g06035-like isoform X2 [Cucurbita moscha...	[more]
XP_008450653.1	2.6e-67	68.88	PREDICTED: uncharacterized GPI-anchored protein At5g19250-like [Cucumis melo]	[more]

BLAST of Carg27609 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G19250.1	7.4e-24	35.03	Glycoprotein membrane precursor GPI-anchored	[more]
AT3G06035.1	9.7e-24	34.18	Glycoprotein membrane precursor GPI-anchored	[more]
AT1G54860.1	1.3e-20	29.90	Glycoprotein membrane precursor GPI-anchored	[more]
AT5G19240.1	2.2e-20	36.97	Glycoprotein membrane precursor GPI-anchored	[more]
AT5G19230.1	1.8e-14	31.31	Glycoprotein membrane precursor GPI-anchored	[more]

BLAST of Carg27609 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P59833\|UGPI3_ARATH	1.3e-22	35.03	Uncharacterized GPI-anchored protein At5g19250 OS=Arabidopsis thaliana OX=3702 G...	[more]
sp\|Q84MC0\|UGPI4_ARATH	1.7e-22	34.18	Uncharacterized GPI-anchored protein At3g06035 OS=Arabidopsis thaliana OX=3702 G...	[more]
sp\|Q84VZ5\|UGPI2_ARATH	4.0e-19	36.97	Uncharacterized GPI-anchored protein At5g19240 OS=Arabidopsis thaliana OX=3702 G...	[more]
sp\|Q8GUL8\|UGPI1_ARATH	3.3e-13	31.31	Uncharacterized GPI-anchored protein At5g19230 OS=Arabidopsis thaliana OX=3702 G...	[more]

BLAST of Carg27609 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BQD0\|A0A1S3BQD0_CUCME	1.7e-67	68.88	uncharacterized GPI-anchored protein At5g19250-like OS=Cucumis melo OX=3656 GN=L...	[more]
tr\|A0A2I4GCP3\|A0A2I4GCP3_9ROSI	1.1e-32	45.28	uncharacterized GPI-anchored protein At3g06035-like OS=Juglans regia OX=51240 GN...	[more]
tr\|M5W0Y4\|M5W0Y4_PRUPE	5.8e-31	42.86	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G327200 PE=4 SV=1	[more]
tr\|A0A2I4GSI9\|A0A2I4GSI9_9ROSI	2.9e-30	39.89	uncharacterized GPI-anchored protein At5g19250-like OS=Juglans regia OX=51240 GN...	[more]
tr\|A0A2P4MBH0\|A0A2P4MBH0_QUESU	1.9e-29	40.80	Putative gpi-anchored protein OS=Quercus suber OX=58331 GN=CFP56_58344 PE=4 SV=1	[more]

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27609-RA	Carg27609-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33976	FAMILY NOT NAMED	coord: 5..192
None	No IPR available	PRODOM	PD208950		coord: 4..164 e-value: 2.0E-28 score: 312.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg27609	Cla005236	Watermelon (97103) v1	carwmB0795
Carg27609	ClCG03G016940	Watermelon (Charleston Gray)	carwcgB0770
Carg27609	CSPI01G26650	Wild cucumber (PI 183967)	carcpiB0833
Carg27609	Cucsa.182110	Cucumber (Gy14) v1	carcgyB0909
Carg27609	CmoCh10G011610	Cucurbita moschata (Rifu)	carcmoB1075
Carg27609	Lsi06G001330	Bottle gourd (USVL1VR-Ls)	carlsiB732
Carg27609	Cp4.1LG18g00660	Cucurbita pepo (Zucchini)	carcpeB1115
Carg27609	CsaV3_1G038990	Cucumber (Chinese Long) v3	carcucB0837
Carg27609	Cla97C03G067310	Watermelon (97103) v2	carwmbB0834

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None