Carg27436 (gene) Silver-seed gourd

NameCarg27436
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionRING-type E3 ubiquitin transferase
LocationCucurbita_argyrosperma_scaffold_494 : 31838 .. 33166 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTCCTCTTGGAGACGACGGAGGGCCACCCGGCGAACGGCAAGGGGCACCGGCAGTGACATGAGAGAGCTGACGATCCCGAGCCATTTCCGGTGTCCAATTTCGCTGGACATGATGAAAGATCCAGTGACTTTGTCTACTGGAATCACATATGATCGTGCTAGTATTGAGACATGGATTGAAGGAGGGAATTTCAGCTGCCCCATCACCAACCAGCCATTACAAACCCTTGATTCCATTCCAAATCATAACATAAGGAAGATGATTCAAGATTGGTGTGTTGAAAATAAAGCTTACGGGGTCGAGAGAATTCCGACGCCGAGGGTTCCGGTGAGTCCCGTGCAGATTCGGGACATTCTCGAGAAGATTACCGCTGCAGCACGGCGGGGGGATGGTGACGGATGCAAGTCGTCGGTCGAGAAGATCAAGATGTTGGCAAAAGAAAGTGAAAGGAACAAGAAATGTATTATGGGCAATGGAGCAAGCCATGTGATATCATCAGCATTCCAAGCGTTTTCAAAACAAGAACCATTGGAGAAAAGCATAGAGATTTTAGAAGAAATGATATCAGCCATGGCAATCATGTTCCCTCTCCAAGAAGAATCCATTAAAAACCTCAAATCAAAAGCTTCATTACAAGCCCTCGTGTTGTTCATGAAAGGAGGAGTTTTATCAAGAAGAAGAAATTCAATTTTGATATTGAAACAAATCATTTCATCATACCCACAAAAAGCAACAAAATTAGGTGAAATCCAAGGAGCATTAGAGGGATTAGTGAAGCTCATAAAAGAGCCCATTTGTTCATCATCAAAAAAAGCCTCACTTTTCATAACATATCACATCATAGCTTCATCTTCCAATCCACAAAGATTCACAAAAGCCCTTTTAGAAATGGGGTTCGTCTCTTTGCTACTCGAAACGCTCGTCGACGCCGAGAGAAGCGTTTGCGAGAGGGCGTTGGGAGCGTTCGATGGGATTTGCGAGAGCAAAGAAGGAAGAGAAGAGGCGTACGCGCACGCGTTGACGATGCCCGTGATAGTGAAAAAGATACTTCGAGTTTCGGATTTGGCGACGGAGTTATCGGTGTCGATTGTTTGGAAGCTTGTAAAGCATGAGAGTGAAGAAGAAGAAGAAGATGGAAGGGTTAAAGTAGAAGCACTTCAAGTGGGAGCGTTTCAAAAGCTATTGTTGTTGTTGCAAGTTGGTTGTAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAACTATTGAATAATCTTCATAGGGATAGCTTAGAATGTATTGATTCATTGGACTTTAAGGGTATCAAAAGACCCTTTTGA

mRNA sequence

ATGATTTCCTCTTGGAGACGACGGAGGGCCACCCGGCGAACGGCAAGGGGCACCGGCAGTGACATGAGAGAGCTGACGATCCCGAGCCATTTCCGGTGTCCAATTTCGCTGGACATGATGAAAGATCCAGTGACTTTGTCTACTGGAATCACATATGATCGTGCTAGTATTGAGACATGGATTGAAGGAGGGAATTTCAGCTGCCCCATCACCAACCAGCCATTACAAACCCTTGATTCCATTCCAAATCATAACATAAGGAAGATGATTCAAGATTGGTGTGTTGAAAATAAAGCTTACGGGGTCGAGAGAATTCCGACGCCGAGGGTTCCGGTGAGTCCCGTGCAGATTCGGGACATTCTCGAGAAGATTACCGCTGCAGCACGGCGGGGGGATGGTGACGGATGCAAGTCGTCGGTCGAGAAGATCAAGATGTTGGCAAAAGAAAGTGAAAGGAACAAGAAATGTATTATGGGCAATGGAGCAAGCCATGTGATATCATCAGCATTCCAAGCGTTTTCAAAACAAGAACCATTGGAGAAAAGCATAGAGATTTTAGAAGAAATGATATCAGCCATGGCAATCATGTTCCCTCTCCAAGAAGAATCCATTAAAAACCTCAAATCAAAAGCTTCATTACAAGCCCTCGTGTTGTTCATGAAAGGAGGAGTTTTATCAAGAAGAAGAAATTCAATTTTGATATTGAAACAAATCATTTCATCATACCCACAAAAAGCAACAAAATTAGGTGAAATCCAAGGAGCATTAGAGGGATTAGTGAAGCTCATAAAAGAGCCCATTTGTTCATCATCAAAAAAAGCCTCACTTTTCATAACATATCACATCATAGCTTCATCTTCCAATCCACAAAGATTCACAAAAGCCCTTTTAGAAATGGGGTTCGTCTCTTTGCTACTCGAAACGCTCGTCGACGCCGAGAGAAGCGTTTGCGAGAGGGCGTTGGGAGCGTTCGATGGGATTTGCGAGAGCAAAGAAGGAAGAGAAGAGGCGTACGCGCACGCGTTGACGATGCCCGTGATAGTGAAAAAGATACTTCGAGTTTCGGATTTGGCGACGGAGTTATCGGTGTCGATTGTTTGGAAGCTTGTAAAGCATGAGAGTGAAGAAGAAGAAGAAGATGGAAGGGTTAAAGTAGAAGCACTTCAAGTGGGAGCGTTTCAAAAGCTATTGTTGTTGTTGCAAGTTGGTTGTAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAACTATTGAATAATCTTCATAGGGATAGCTTAGAATGTATTGATTCATTGGACTTTAAGGGTATCAAAAGACCCTTTTGA

Coding sequence (CDS)

ATGATTTCCTCTTGGAGACGACGGAGGGCCACCCGGCGAACGGCAAGGGGCACCGGCAGTGACATGAGAGAGCTGACGATCCCGAGCCATTTCCGGTGTCCAATTTCGCTGGACATGATGAAAGATCCAGTGACTTTGTCTACTGGAATCACATATGATCGTGCTAGTATTGAGACATGGATTGAAGGAGGGAATTTCAGCTGCCCCATCACCAACCAGCCATTACAAACCCTTGATTCCATTCCAAATCATAACATAAGGAAGATGATTCAAGATTGGTGTGTTGAAAATAAAGCTTACGGGGTCGAGAGAATTCCGACGCCGAGGGTTCCGGTGAGTCCCGTGCAGATTCGGGACATTCTCGAGAAGATTACCGCTGCAGCACGGCGGGGGGATGGTGACGGATGCAAGTCGTCGGTCGAGAAGATCAAGATGTTGGCAAAAGAAAGTGAAAGGAACAAGAAATGTATTATGGGCAATGGAGCAAGCCATGTGATATCATCAGCATTCCAAGCGTTTTCAAAACAAGAACCATTGGAGAAAAGCATAGAGATTTTAGAAGAAATGATATCAGCCATGGCAATCATGTTCCCTCTCCAAGAAGAATCCATTAAAAACCTCAAATCAAAAGCTTCATTACAAGCCCTCGTGTTGTTCATGAAAGGAGGAGTTTTATCAAGAAGAAGAAATTCAATTTTGATATTGAAACAAATCATTTCATCATACCCACAAAAAGCAACAAAATTAGGTGAAATCCAAGGAGCATTAGAGGGATTAGTGAAGCTCATAAAAGAGCCCATTTGTTCATCATCAAAAAAAGCCTCACTTTTCATAACATATCACATCATAGCTTCATCTTCCAATCCACAAAGATTCACAAAAGCCCTTTTAGAAATGGGGTTCGTCTCTTTGCTACTCGAAACGCTCGTCGACGCCGAGAGAAGCGTTTGCGAGAGGGCGTTGGGAGCGTTCGATGGGATTTGCGAGAGCAAAGAAGGAAGAGAAGAGGCGTACGCGCACGCGTTGACGATGCCCGTGATAGTGAAAAAGATACTTCGAGTTTCGGATTTGGCGACGGAGTTATCGGTGTCGATTGTTTGGAAGCTTGTAAAGCATGAGAGTGAAGAAGAAGAAGAAGATGGAAGGGTTAAAGTAGAAGCACTTCAAGTGGGAGCGTTTCAAAAGCTATTGTTGTTGTTGCAAGTTGGTTGTAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAACTATTGAATAATCTTCATAGGGATAGCTTAGAATGTATTGATTCATTGGACTTTAAGGGTATCAAAAGACCCTTTTGA

Protein sequence

MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWIEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLEKSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIISSYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDSLECIDSLDFKGIKRPF
BLAST of Carg27436 vs. NCBI nr
Match: XP_023512745.1 (U-box domain-containing protein 21-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 842.0 bits (2174), Expect = 9.0e-241
Identity = 436/442 (98.64%), Postives = 437/442 (98.87%), Query Frame = 0

Query: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60
           MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60

Query: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120
           IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENK YGVERIPTPRVPVSPVQI DI
Sbjct: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKDYGVERIPTPRVPVSPVQIGDI 120

Query: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLE 180
           LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQA SKQEPLE
Sbjct: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQALSKQEPLE 180

Query: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIIS 240
           KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGG LSRRRNSILILK+IIS
Sbjct: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGDLSRRRNSILILKEIIS 240

Query: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMG 300
           SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMG
Sbjct: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMG 300

Query: 301 FVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE 360
           FVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE
Sbjct: 301 FVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE 360

Query: 361 LSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420
           LSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN
Sbjct: 361 LSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420

Query: 421 NLHRDSLECIDSLDFKGIKRPF 443
           NLHRD LECIDSLDFKGIKRPF
Sbjct: 421 NLHRDRLECIDSLDFKGIKRPF 442

BLAST of Carg27436 vs. NCBI nr
Match: XP_022942705.1 (U-box domain-containing protein 21-like [Cucurbita moschata])

HSP 1 Score: 833.6 bits (2152), Expect = 3.2e-238
Identity = 433/442 (97.96%), Postives = 435/442 (98.42%), Query Frame = 0

Query: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60
           MISSWR+RRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRQRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60

Query: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120
           IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI
Sbjct: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120

Query: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLE 180
           LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQA SKQEPLE
Sbjct: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQALSKQEPLE 180

Query: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIIS 240
           KSIEILEEMISAMAIMFPLQEESIKNLKS+ASLQALVLFMKGG LSRRRNSILILKQIIS
Sbjct: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSEASLQALVLFMKGGDLSRRRNSILILKQIIS 240

Query: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMG 300
           SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTK LLEMG
Sbjct: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKVLLEMG 300

Query: 301 FVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE 360
           FVSLLLETLVDAERSVCERALGAFDGICE KEGREEAYAHALTMPVIVKKILRVSDLATE
Sbjct: 301 FVSLLLETLVDAERSVCERALGAFDGICERKEGREEAYAHALTMPVIVKKILRVSDLATE 360

Query: 361 LSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420
           LSVSIVWKLVKHES  EEEDGR KVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN
Sbjct: 361 LSVSIVWKLVKHES--EEEDGRAKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420

Query: 421 NLHRDSLECIDSLDFKGIKRPF 443
           NLHRDSLECIDSLDFKGIKRPF
Sbjct: 421 NLHRDSLECIDSLDFKGIKRPF 440

BLAST of Carg27436 vs. NCBI nr
Match: XP_022995728.1 (U-box domain-containing protein 21-like [Cucurbita maxima])

HSP 1 Score: 817.8 bits (2111), Expect = 1.8e-233
Identity = 423/442 (95.70%), Postives = 429/442 (97.06%), Query Frame = 0

Query: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60
           MISSWRRRRATRRTARGTG  MRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRRRRATRRTARGTGGCMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60

Query: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120
           IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVP SPVQIRDI
Sbjct: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPASPVQIRDI 120

Query: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLE 180
           L+KITAA RRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQA SKQEPLE
Sbjct: 121 LDKITAATRRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQALSKQEPLE 180

Query: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIIS 240
           KSIEILEEMIS MAIMFPLQEESIKNLKS+ASLQAL LFM+GG LSRRRNSILILK+IIS
Sbjct: 181 KSIEILEEMISGMAIMFPLQEESIKNLKSEASLQALALFMRGGDLSRRRNSILILKEIIS 240

Query: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMG 300
           SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALL+MG
Sbjct: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLQMG 300

Query: 301 FVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE 360
           FVSLLLETLVDAERS+CERALG FDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE
Sbjct: 301 FVSLLLETLVDAERSICERALGVFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE 360

Query: 361 LSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420
           LSVSIVWKLVKHES  EEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN
Sbjct: 361 LSVSIVWKLVKHES--EEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420

Query: 421 NLHRDSLECIDSLDFKGIKRPF 443
           NLHRD LECIDSLDFK IKRPF
Sbjct: 421 NLHRDRLECIDSLDFKDIKRPF 440

BLAST of Carg27436 vs. NCBI nr
Match: XP_004142305.1 (PREDICTED: U-box domain-containing protein 21-like [Cucumis sativus] >KGN49607.1 hypothetical protein Csa_5G023870 [Cucumis sativus])

HSP 1 Score: 714.9 bits (1844), Expect = 1.7e-202
Identity = 364/445 (81.80%), Postives = 401/445 (90.11%), Query Frame = 0

Query: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60
           MISSWRRRRATRR ARG  S   ELTIP+HFRCPISLD+MKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRRRRATRRMARGGLSHTIELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIETW 60

Query: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120
           IEGGNF+CP TNQPLQT+DSIPNHNIRKMIQDWCVEN+AYG+ERIPTPRVP SP Q+R+I
Sbjct: 61  IEGGNFTCPFTNQPLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREI 120

Query: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLE 180
           LEK+ AAARRGD DGCKS VEKIK L KESERNKKCI+ NG  + +SS F+AFS  E  E
Sbjct: 121 LEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGNTLSSIFEAFSNPETFE 180

Query: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIIS 240
           K IEILEE++SAM + FPLQEESIK+LKS+ SLQ+LV F+KGG +S RRNS+L+LK+IIS
Sbjct: 181 KRIEILEEILSAMTVAFPLQEESIKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIIS 240

Query: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIA---SSSNPQRFTKALL 300
           SYP+K  +LGEIQGALEGL+KLIK+PICSSS+KASLFITYH+IA   SSSN +RFTK LL
Sbjct: 241 SYPEKVDELGEIQGALEGLIKLIKDPICSSSRKASLFITYHVIASTSSSSNRERFTKELL 300

Query: 301 EMGFVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDL 360
           +MG VSLLLETLVDAERSVCERALGAFDGICE+K+GREEAYAHALTMPVIVKKILRVSDL
Sbjct: 301 QMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDL 360

Query: 361 ATELSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLK 420
           ATELSV IVWKLVKHES +EEEDG +KVEALQVGAFQKLLLLLQVGCSEWTKEKATELLK
Sbjct: 361 ATELSVCIVWKLVKHES-KEEEDGGIKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLK 420

Query: 421 LLNNLHRDSLECIDSLDFKGIKRPF 443
           LLNNLHRD LECIDSLDFK +KR F
Sbjct: 421 LLNNLHRDRLECIDSLDFKDLKRTF 444

BLAST of Carg27436 vs. NCBI nr
Match: XP_008465536.1 (PREDICTED: U-box domain-containing protein 21-like [Cucumis melo])

HSP 1 Score: 713.0 bits (1839), Expect = 6.3e-202
Identity = 362/445 (81.35%), Postives = 401/445 (90.11%), Query Frame = 0

Query: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60
           MISSWRRRRAT R  RG+  D  ELTIP+HFRCPISLD+MKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRRRRATPRMPRGSLGDPVELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIETW 60

Query: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120
           IEGGNF+CP TNQPLQT+DSIPNHNIRKMIQDWCVEN+AYG+ERIPTPRVP SP Q+RDI
Sbjct: 61  IEGGNFTCPFTNQPLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRDI 120

Query: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLE 180
           LEK+TAAARRGD +GCKS VEKIK L KESERNKKCI+ NG  +  SS F+AFS  E  E
Sbjct: 121 LEKMTAAARRGDFEGCKSMVEKIKNLGKESERNKKCIIANGTGNTFSSIFEAFSNPETFE 180

Query: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIIS 240
           K IEILEE++SAM ++FPLQEESIK+LKS+ SLQ+L  F+KGG +S RRNS+L+LK+IIS
Sbjct: 181 KRIEILEEILSAMTMVFPLQEESIKHLKSETSLQSLAWFLKGGDISGRRNSVLVLKEIIS 240

Query: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIA---SSSNPQRFTKALL 300
           SYP+K  +LG IQGALEGL+KLIK+PICSSSKKASLFITYH+IA   SSSN  RFTKALL
Sbjct: 241 SYPEKVDELGVIQGALEGLIKLIKDPICSSSKKASLFITYHVIASTSSSSNQGRFTKALL 300

Query: 301 EMGFVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDL 360
           +MG VSLLLETLVDAERSVCERALGAFDGICE+K+GREEAYAHALTMPVIVKKILRVSDL
Sbjct: 301 QMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDL 360

Query: 361 ATELSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLK 420
           ATELSVSIVWKLVK+ES+EE+EDG ++VEALQVGAFQKLLLLLQVGCSEWTKEKATELLK
Sbjct: 361 ATELSVSIVWKLVKNESKEEKEDGGIEVEALQVGAFQKLLLLLQVGCSEWTKEKATELLK 420

Query: 421 LLNNLHRDSLECIDSLDFKGIKRPF 443
           LLNNLHRD LECIDSLDFK +KR F
Sbjct: 421 LLNNLHRDRLECIDSLDFKDLKRTF 445

BLAST of Carg27436 vs. TAIR10
Match: AT5G37490.1 (ARM repeat superfamily protein)

HSP 1 Score: 361.3 bits (926), Expect = 8.5e-100
Identity = 198/420 (47.14%), Postives = 269/420 (64.05%), Query Frame = 0

Query: 24  ELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWIEGGNFSCPITNQPLQTLDSIPN 83
           E+TIP  F+CPIS+D+MKDPV +STGITYDR SIETWI  GN +CP+TN  L T D IPN
Sbjct: 28  EITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPN 87

Query: 84  HNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKSSVEKI 143
           H IRKMIQ WCVE  +  ++RIPTPRVP+ P ++ +I  K+++A RRGD + C   +EKI
Sbjct: 88  HTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEKI 147

Query: 144 KMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLEKSIEILEEMISAMAIMFPLQEES 203
           K L  ESE+N+KC+  N    V+   F  FS     EK   +L E++S +  MFP+  E 
Sbjct: 148 KKLGDESEKNRKCVNENSVGWVLCDCFDKFSGD---EKLTFMLNEILSLLTWMFPIGLEG 207

Query: 204 IKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIISSYPQKATKLGEIQGALEGLVKLI 263
           I  L S  S + +   +K    S R+N+  I+K+I+S    +        G  E LVKLI
Sbjct: 208 ISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAEALVKLI 267

Query: 264 KEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETLVDAERSVCERALGA 323
           ++ + SSS K+SL   Y ++             LE+G VS+ +E +VDAE SVCE+AL  
Sbjct: 268 RDSVSSSSTKSSLIAIYQMVLQK---PEIASEFLEIGLVSITVEMIVDAENSVCEKALAV 327

Query: 324 FDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESEEEEEDGRV 383
            D ICE++ GREE   +AL MP++VKKI +VS+LAT  S+S++ KL K  +    ED   
Sbjct: 328 LDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVAVED--- 387

Query: 384 KVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDSLECIDSL-DFKGIKRPF 443
              A+++GAFQK+LL+LQVG  E TKEKATELLK++N   +   +C+DSL +FK IK+PF
Sbjct: 388 ---AVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCVDSLKEFKYIKKPF 435

BLAST of Carg27436 vs. TAIR10
Match: AT1G66160.1 (CYS, MET, PRO, and GLY protein 1)

HSP 1 Score: 313.2 bits (801), Expect = 2.6e-85
Identity = 177/427 (41.45%), Postives = 262/427 (61.36%), Query Frame = 0

Query: 20  SDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWIEGGNFSCPITNQPLQTLD 79
           S+  E+TIPS F+CPIS ++MKDPV +++GITYDR +IE W E G  +CP+TN  L +L+
Sbjct: 26  SEEVEITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLE 85

Query: 80  SIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKSS 139
            IPNH IR+MIQ WC  +   G+ERIPTPRVPV+  Q+ +I E+++AA RRGD   C   
Sbjct: 86  QIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEM 145

Query: 140 VEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLEKSIEILEEMISAMAIMFPL 199
           V K+  L KESERN+KC+  NGA  V+   F AFS+      +  +LEE +S +  M P+
Sbjct: 146 VTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFSEN---ANASLLLEETVSVLTWMLPI 205

Query: 200 QEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIISSYPQKATKLGEIQGALEGL 259
             E    L + +S   LV  ++ G     +N+  ++K+++         L +I G  E  
Sbjct: 206 GLEGQSKLTTTSSFNRLVELLRNG----DQNAAFLIKELLELNVTHVHALTKINGVQEAF 265

Query: 260 VKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETLVDAERSVCER 319
           +K I      S+   SL   +H+I ++   Q      LE+  V++ +E LVD+E SVCE+
Sbjct: 266 MKSINR---DSTCVNSLISIHHMILTN---QETVSRFLELDLVNITVEMLVDSENSVCEK 325

Query: 320 ALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESEEEEE 379
           AL   + ICE+KEGRE+   + L +P++VKKIL++S+   +  VS++WK+ K        
Sbjct: 326 ALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK------SG 385

Query: 380 DGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDS----LECIDSLDF 439
           DG    EAL++GAF+KL+++LQVGC E TKEK TELLK++N + + +         S++F
Sbjct: 386 DGSEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDRSYSSSIEF 431

Query: 440 KGIKRPF 443
           K +K+PF
Sbjct: 446 KHVKKPF 431

BLAST of Carg27436 vs. TAIR10
Match: AT3G02840.1 (ARM repeat superfamily protein)

HSP 1 Score: 235.0 bits (598), Expect = 9.2e-62
Identity = 139/346 (40.17%), Postives = 222/346 (64.16%), Query Frame = 0

Query: 102 VERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNG 161
           +ERI +PRV ++P    +I  ++  AA R +   C   V KIK L +  + NKKC++ NG
Sbjct: 40  IERIQSPRVLLTPRDAVEISRRLQNAAAREEYAECLEIVSKIKNLGRGGDTNKKCLVQNG 99

Query: 162 ASHVISSAFQAFSKQEPLEKSIEILEEMISAMAIMFPL-QEESIKNLKSKASLQALVLFM 221
           +   +SS F+ F+     +  + +LEE++  ++   PL + E    + S ASL  LV F+
Sbjct: 100 SVLALSSCFERFAAAR--DGHMRLLEEILFVLSSWLPLNRSEGFNKMGSTASLNCLVRFL 159

Query: 222 KGGVLSRRRNSILILKQIISSYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITY 281
            G     R+N+   ++++I+   +    L +++GA EGLVK+I++ + +SS KASL + Y
Sbjct: 160 NGKDAKTRQNAAFCIREVIAVDKRYVYALTDVEGACEGLVKIIRDSVSTSSTKASLMVIY 219

Query: 282 HIIASSSNPQRFTKALLEMGFVSLLLETLV-DAERSVCERALGAFDGICESKEGREEAYA 341
             I+S+    + T+  +++G V L+ E +V +AE+SVCER+L   +  C++++G+E+   
Sbjct: 220 RAISSN---DKITEKFVKLGLVELITEMMVNNAEKSVCERSLVVLNATCDNEQGKEDVLR 279

Query: 342 HALTMPVIVKKILRVSDLATELSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLL 401
           +AL +P++VKKILRVSDLAT+ SVSI+WKL K++ + E +D R+ VEALQVGAF+KLL+L
Sbjct: 280 NALIVPLLVKKILRVSDLATQCSVSILWKLWKNKKDGECDD-RLLVEALQVGAFEKLLVL 339

Query: 402 LQVGCSEWTKEKATELLKLLNNLHR--DSLECID-SLDFKGIKRPF 443
           LQVGC + TKEKA+ELL+ LN      +   C+D S+  K +K+ F
Sbjct: 340 LQVGCEDKTKEKASELLRNLNRCRNEIEKTNCVDSSMHLKNVKKSF 379

BLAST of Carg27436 vs. TAIR10
Match: AT1G49780.1 (plant U-box 26)

HSP 1 Score: 213.8 bits (543), Expect = 2.2e-55
Identity = 144/419 (34.37%), Postives = 223/419 (53.22%), Query Frame = 0

Query: 25  LTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWIEGGNFSCPITNQPLQTLDSIPNH 84
           + IP HFRCPISLD+M DPVT+STG TYDR SI++WI  GN +CP+T   L     IPNH
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSDFTLIPNH 71

Query: 85  NIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKS---SVE 144
            +R++IQ+WCV N++ GVERIPTP+ P  P+ +R +L +  A+A  G     +S   ++ 
Sbjct: 72  TLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQ--ASAITGTHVSVRSRAAAIR 131

Query: 145 KIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLEKSIEILEEMISAMAI--MFPL 204
           +++ LA++SE+N+  I G+ A  ++      F+  E    S E++ E ++ + +  M   
Sbjct: 132 RLRGLARDSEKNRVLIAGHNAREILVRIL--FADIETTSLSSELVSESLALLVLLHMTET 191

Query: 205 QEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIISSYPQKATKL--GEIQGALE 264
           + E++ +  S+      +LF     +  R N+  +++ +++       KL         E
Sbjct: 192 ECEAVASDPSRVGFMTRLLF--DSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFE 251

Query: 265 GLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETL-VDAERSV 324
           G++ L+K PI   S + +L I    I +    ++     +  G   +L++ L  D +R  
Sbjct: 252 GVLDLLKNPI---SSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCD 311

Query: 325 CERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESEE 384
            ER L   + +C   EG      HALT+P++VK ILRVSD ATE +   +  L   E   
Sbjct: 312 TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCTAEE-- 371

Query: 385 EEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDSLECIDSLDF 436
                R + EA   G   +LLLL+Q  C+E  K KA  LLKLL +   D    + S DF
Sbjct: 372 -----RCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDD-STVHSDDF 413

BLAST of Carg27436 vs. TAIR10
Match: AT3G19380.1 (plant U-box 25)

HSP 1 Score: 208.8 bits (530), Expect = 7.0e-54
Identity = 142/421 (33.73%), Postives = 230/421 (54.63%), Query Frame = 0

Query: 25  LTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWIE-GGNFSCPITNQPLQTLDSIPN 84
           + IP HFRCPISL++M+DPVT+ TG TYDRASIE+W+  G N +CP+T  PL     IPN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 85  HNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKS---SV 144
           H +R++IQ+WCV N++ GVERIPTP+ P  P  +R +L +  A+A  G     +S   ++
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQ--ASAITGTHVSVRSRAAAL 131

Query: 145 EKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLEKSIEILEEMISAMAIMFPLQ 204
            +++  A++S++N+  I  + A+ ++      FS+      S E++ E + A+ +M P+ 
Sbjct: 132 RRLRGFARDSDKNRVLIAAHNATEILIKIL--FSE----TTSSELVSESL-ALLVMLPIT 191

Query: 205 E--ESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIISSYPQKATKLGEI---QGA 264
           E  + +        ++ L   +    +  R N+  ++ +I+S+  + A   G I   +  
Sbjct: 192 EPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALI-EIVSTGTKSADLKGSISNSESV 251

Query: 265 LEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETL-VDAER 324
            EG++ L++ PI   S + +L I    + +  + +      +  G   +L++ L  D +R
Sbjct: 252 FEGVLDLLRNPI---SSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDR 311

Query: 325 SVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHES 384
              ERAL   + +C + EG      HALT+P++VK ILRVSD ATE +   +  L   E 
Sbjct: 312 CDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCTAEE 371

Query: 385 EEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDSLECIDSLD 436
                  R + EA   G   +LLL++Q  C+E  K+KA +LLKLL +   D     +S D
Sbjct: 372 -------RWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYNSFANSDD 412

BLAST of Carg27436 vs. Swiss-Prot
Match: sp|Q5PNY6|PUB21_ARATH (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.5e-98
Identity = 198/420 (47.14%), Postives = 269/420 (64.05%), Query Frame = 0

Query: 24  ELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWIEGGNFSCPITNQPLQTLDSIPN 83
           E+TIP  F+CPIS+D+MKDPV +STGITYDR SIETWI  GN +CP+TN  L T D IPN
Sbjct: 28  EITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPN 87

Query: 84  HNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKSSVEKI 143
           H IRKMIQ WCVE  +  ++RIPTPRVP+ P ++ +I  K+++A RRGD + C   +EKI
Sbjct: 88  HTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEKI 147

Query: 144 KMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLEKSIEILEEMISAMAIMFPLQEES 203
           K L  ESE+N+KC+  N    V+   F  FS     EK   +L E++S +  MFP+  E 
Sbjct: 148 KKLGDESEKNRKCVNENSVGWVLCDCFDKFSGD---EKLTFMLNEILSLLTWMFPIGLEG 207

Query: 204 IKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIISSYPQKATKLGEIQGALEGLVKLI 263
           I  L S  S + +   +K    S R+N+  I+K+I+S    +        G  E LVKLI
Sbjct: 208 ISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAEALVKLI 267

Query: 264 KEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETLVDAERSVCERALGA 323
           ++ + SSS K+SL   Y ++             LE+G VS+ +E +VDAE SVCE+AL  
Sbjct: 268 RDSVSSSSTKSSLIAIYQMVLQK---PEIASEFLEIGLVSITVEMIVDAENSVCEKALAV 327

Query: 324 FDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESEEEEEDGRV 383
            D ICE++ GREE   +AL MP++VKKI +VS+LAT  S+S++ KL K  +    ED   
Sbjct: 328 LDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVAVED--- 387

Query: 384 KVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDSLECIDSL-DFKGIKRPF 443
              A+++GAFQK+LL+LQVG  E TKEKATELLK++N   +   +C+DSL +FK IK+PF
Sbjct: 388 ---AVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCVDSLKEFKYIKKPF 435

BLAST of Carg27436 vs. Swiss-Prot
Match: sp|Q9C8D1|PUB20_ARATH (U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=2 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 4.8e-84
Identity = 177/427 (41.45%), Postives = 262/427 (61.36%), Query Frame = 0

Query: 20  SDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWIEGGNFSCPITNQPLQTLD 79
           S+  E+TIPS F+CPIS ++MKDPV +++GITYDR +IE W E G  +CP+TN  L +L+
Sbjct: 26  SEEVEITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLE 85

Query: 80  SIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKSS 139
            IPNH IR+MIQ WC  +   G+ERIPTPRVPV+  Q+ +I E+++AA RRGD   C   
Sbjct: 86  QIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEM 145

Query: 140 VEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLEKSIEILEEMISAMAIMFPL 199
           V K+  L KESERN+KC+  NGA  V+   F AFS+      +  +LEE +S +  M P+
Sbjct: 146 VTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFSEN---ANASLLLEETVSVLTWMLPI 205

Query: 200 QEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIISSYPQKATKLGEIQGALEGL 259
             E    L + +S   LV  ++ G     +N+  ++K+++         L +I G  E  
Sbjct: 206 GLEGQSKLTTTSSFNRLVELLRNG----DQNAAFLIKELLELNVTHVHALTKINGVQEAF 265

Query: 260 VKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETLVDAERSVCER 319
           +K I      S+   SL   +H+I ++   Q      LE+  V++ +E LVD+E SVCE+
Sbjct: 266 MKSINR---DSTCVNSLISIHHMILTN---QETVSRFLELDLVNITVEMLVDSENSVCEK 325

Query: 320 ALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESEEEEE 379
           AL   + ICE+KEGRE+   + L +P++VKKIL++S+   +  VS++WK+ K        
Sbjct: 326 ALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK------SG 385

Query: 380 DGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDS----LECIDSLDF 439
           DG    EAL++GAF+KL+++LQVGC E TKEK TELLK++N + + +         S++F
Sbjct: 386 DGSEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDRSYSSSIEF 431

Query: 440 KGIKRPF 443
           K +K+PF
Sbjct: 446 KHVKKPF 431

BLAST of Carg27436 vs. Swiss-Prot
Match: sp|Q9FXA4|PUB26_ARATH (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 3.9e-54
Identity = 144/419 (34.37%), Postives = 223/419 (53.22%), Query Frame = 0

Query: 25  LTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWIEGGNFSCPITNQPLQTLDSIPNH 84
           + IP HFRCPISLD+M DPVT+STG TYDR SI++WI  GN +CP+T   L     IPNH
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSDFTLIPNH 71

Query: 85  NIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKS---SVE 144
            +R++IQ+WCV N++ GVERIPTP+ P  P+ +R +L +  A+A  G     +S   ++ 
Sbjct: 72  TLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQ--ASAITGTHVSVRSRAAAIR 131

Query: 145 KIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLEKSIEILEEMISAMAI--MFPL 204
           +++ LA++SE+N+  I G+ A  ++      F+  E    S E++ E ++ + +  M   
Sbjct: 132 RLRGLARDSEKNRVLIAGHNAREILVRIL--FADIETTSLSSELVSESLALLVLLHMTET 191

Query: 205 QEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIISSYPQKATKL--GEIQGALE 264
           + E++ +  S+      +LF     +  R N+  +++ +++       KL         E
Sbjct: 192 ECEAVASDPSRVGFMTRLLF--DSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFE 251

Query: 265 GLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETL-VDAERSV 324
           G++ L+K PI   S + +L I    I +    ++     +  G   +L++ L  D +R  
Sbjct: 252 GVLDLLKNPI---SSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCD 311

Query: 325 CERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESEE 384
            ER L   + +C   EG      HALT+P++VK ILRVSD ATE +   +  L   E   
Sbjct: 312 TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCTAEE-- 371

Query: 385 EEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDSLECIDSLDF 436
                R + EA   G   +LLLL+Q  C+E  K KA  LLKLL +   D    + S DF
Sbjct: 372 -----RCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDD-STVHSDDF 413

BLAST of Carg27436 vs. Swiss-Prot
Match: sp|Q9LT79|PUB25_ARATH (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.3e-52
Identity = 142/421 (33.73%), Postives = 230/421 (54.63%), Query Frame = 0

Query: 25  LTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWIE-GGNFSCPITNQPLQTLDSIPN 84
           + IP HFRCPISL++M+DPVT+ TG TYDRASIE+W+  G N +CP+T  PL     IPN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 85  HNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKS---SV 144
           H +R++IQ+WCV N++ GVERIPTP+ P  P  +R +L +  A+A  G     +S   ++
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQ--ASAITGTHVSVRSRAAAL 131

Query: 145 EKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLEKSIEILEEMISAMAIMFPLQ 204
            +++  A++S++N+  I  + A+ ++      FS+      S E++ E + A+ +M P+ 
Sbjct: 132 RRLRGFARDSDKNRVLIAAHNATEILIKIL--FSE----TTSSELVSESL-ALLVMLPIT 191

Query: 205 E--ESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIISSYPQKATKLGEI---QGA 264
           E  + +        ++ L   +    +  R N+  ++ +I+S+  + A   G I   +  
Sbjct: 192 EPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALI-EIVSTGTKSADLKGSISNSESV 251

Query: 265 LEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETL-VDAER 324
            EG++ L++ PI   S + +L I    + +  + +      +  G   +L++ L  D +R
Sbjct: 252 FEGVLDLLRNPI---SSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDR 311

Query: 325 SVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHES 384
              ERAL   + +C + EG      HALT+P++VK ILRVSD ATE +   +  L   E 
Sbjct: 312 CDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCTAEE 371

Query: 385 EEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDSLECIDSLD 436
                  R + EA   G   +LLL++Q  C+E  K+KA +LLKLL +   D     +S D
Sbjct: 372 -------RWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYNSFANSDD 412

BLAST of Carg27436 vs. Swiss-Prot
Match: sp|Q84TG3|PUB23_ARATH (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 4.5e-50
Identity = 134/411 (32.60%), Postives = 222/411 (54.01%), Query Frame = 0

Query: 24  ELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWI-EGGNFSCPITNQPLQTLDSIP 83
           E+ IP  F CPISL++MKDPV +STGITYDR SIE W+  G   SCP+T Q +   D  P
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTP 68

Query: 84  NHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKSSVEK 143
           NH +R++IQ WC  N +YGVERIPTPR P+   +I  ++    +A+   +   C   +++
Sbjct: 69  NHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIR--DSASSHENQVKC---LKR 128

Query: 144 IKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPL-EKSIEILEEMISAMAIMFPLQE 203
           ++ +  E+  NK+C+   G    +++     S+   L ++++ +L  + ++  ++    +
Sbjct: 129 LRQIVSENATNKRCLEAAGVPEFLANIVSNDSENGSLTDEALNLLYHLETSETVL----K 188

Query: 204 ESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQI--ISSYPQKATKLGEIQGALEGL 263
             + N K    +++L   M+ G+   R  + L+LK I  ++   Q  T   E+      +
Sbjct: 189 NLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEV---FTEV 248

Query: 264 VKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETLVD----AERS 323
           V+++ + I   + KA++ I  +I     N  +     +E G +S+++E L+D    +ER 
Sbjct: 249 VQILDDRISQKATKAAMHILVNICPWGRNRHK----AVEAGVISVIIELLMDESFTSERR 308

Query: 324 VCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESE 383
             E A+   D +C+  EGR E   H   + V+ KKILRVS  A++ +V ++  + +  + 
Sbjct: 309 GPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCAT 368

Query: 384 EEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDS 427
                  +  E LQ+G   KL L+LQV C   TKEKA ELLKL   + +DS
Sbjct: 369 P-----ALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDS 398

BLAST of Carg27436 vs. TrEMBL
Match: tr|A0A0A0KMI8|A0A0A0KMI8_CUCSA (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G023870 PE=4 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 1.1e-202
Identity = 364/445 (81.80%), Postives = 401/445 (90.11%), Query Frame = 0

Query: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60
           MISSWRRRRATRR ARG  S   ELTIP+HFRCPISLD+MKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRRRRATRRMARGGLSHTIELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIETW 60

Query: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120
           IEGGNF+CP TNQPLQT+DSIPNHNIRKMIQDWCVEN+AYG+ERIPTPRVP SP Q+R+I
Sbjct: 61  IEGGNFTCPFTNQPLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREI 120

Query: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLE 180
           LEK+ AAARRGD DGCKS VEKIK L KESERNKKCI+ NG  + +SS F+AFS  E  E
Sbjct: 121 LEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGNTLSSIFEAFSNPETFE 180

Query: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIIS 240
           K IEILEE++SAM + FPLQEESIK+LKS+ SLQ+LV F+KGG +S RRNS+L+LK+IIS
Sbjct: 181 KRIEILEEILSAMTVAFPLQEESIKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIIS 240

Query: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIA---SSSNPQRFTKALL 300
           SYP+K  +LGEIQGALEGL+KLIK+PICSSS+KASLFITYH+IA   SSSN +RFTK LL
Sbjct: 241 SYPEKVDELGEIQGALEGLIKLIKDPICSSSRKASLFITYHVIASTSSSSNRERFTKELL 300

Query: 301 EMGFVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDL 360
           +MG VSLLLETLVDAERSVCERALGAFDGICE+K+GREEAYAHALTMPVIVKKILRVSDL
Sbjct: 301 QMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDL 360

Query: 361 ATELSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLK 420
           ATELSV IVWKLVKHES +EEEDG +KVEALQVGAFQKLLLLLQVGCSEWTKEKATELLK
Sbjct: 361 ATELSVCIVWKLVKHES-KEEEDGGIKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLK 420

Query: 421 LLNNLHRDSLECIDSLDFKGIKRPF 443
           LLNNLHRD LECIDSLDFK +KR F
Sbjct: 421 LLNNLHRDRLECIDSLDFKDLKRTF 444

BLAST of Carg27436 vs. TrEMBL
Match: tr|A0A1S3CPH4|A0A1S3CPH4_CUCME (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103503164 PE=4 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 4.2e-202
Identity = 362/445 (81.35%), Postives = 401/445 (90.11%), Query Frame = 0

Query: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60
           MISSWRRRRAT R  RG+  D  ELTIP+HFRCPISLD+MKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRRRRATPRMPRGSLGDPVELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIETW 60

Query: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120
           IEGGNF+CP TNQPLQT+DSIPNHNIRKMIQDWCVEN+AYG+ERIPTPRVP SP Q+RDI
Sbjct: 61  IEGGNFTCPFTNQPLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRDI 120

Query: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLE 180
           LEK+TAAARRGD +GCKS VEKIK L KESERNKKCI+ NG  +  SS F+AFS  E  E
Sbjct: 121 LEKMTAAARRGDFEGCKSMVEKIKNLGKESERNKKCIIANGTGNTFSSIFEAFSNPETFE 180

Query: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIIS 240
           K IEILEE++SAM ++FPLQEESIK+LKS+ SLQ+L  F+KGG +S RRNS+L+LK+IIS
Sbjct: 181 KRIEILEEILSAMTMVFPLQEESIKHLKSETSLQSLAWFLKGGDISGRRNSVLVLKEIIS 240

Query: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIA---SSSNPQRFTKALL 300
           SYP+K  +LG IQGALEGL+KLIK+PICSSSKKASLFITYH+IA   SSSN  RFTKALL
Sbjct: 241 SYPEKVDELGVIQGALEGLIKLIKDPICSSSKKASLFITYHVIASTSSSSNQGRFTKALL 300

Query: 301 EMGFVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDL 360
           +MG VSLLLETLVDAERSVCERALGAFDGICE+K+GREEAYAHALTMPVIVKKILRVSDL
Sbjct: 301 QMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDL 360

Query: 361 ATELSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLK 420
           ATELSVSIVWKLVK+ES+EE+EDG ++VEALQVGAFQKLLLLLQVGCSEWTKEKATELLK
Sbjct: 361 ATELSVSIVWKLVKNESKEEKEDGGIEVEALQVGAFQKLLLLLQVGCSEWTKEKATELLK 420

Query: 421 LLNNLHRDSLECIDSLDFKGIKRPF 443
           LLNNLHRD LECIDSLDFK +KR F
Sbjct: 421 LLNNLHRDRLECIDSLDFKDLKRTF 445

BLAST of Carg27436 vs. TrEMBL
Match: tr|A0A2I4G200|A0A2I4G200_9ROSI (RING-type E3 ubiquitin transferase OS=Juglans regia OX=51240 GN=LOC109003997 PE=4 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 9.8e-151
Identity = 284/445 (63.82%), Postives = 350/445 (78.65%), Query Frame = 0

Query: 1   MISSWRRRRATRRTARGTG---SDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASI 60
           MISSWRRRRA RR A+  G   +   ELTIP+HFRC ISLD+MKDPVTLSTGITYDR SI
Sbjct: 1   MISSWRRRRAGRRAAKDQGRGENGDSELTIPTHFRCSISLDLMKDPVTLSTGITYDRESI 60

Query: 61  ETWIEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQI 120
           ETWIE GN +CPI+NQ L++L+ IPNH IRKMIQDWCV+N++YG+ERIPTPR+PVS V +
Sbjct: 61  ETWIEAGNKTCPISNQALKSLEPIPNHAIRKMIQDWCVDNQSYGIERIPTPRIPVSSVVV 120

Query: 121 RDILEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQE 180
            +IL +IT A++RGD + C   V KIK L KESERNK+CIM N    ++S+AF+ F+K  
Sbjct: 121 AEILSEITMASKRGDQEVCLQLVAKIKALWKESERNKRCIMANNTGSLLSAAFETFAKAS 180

Query: 181 PLEKSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQ 240
              ++  +LEE++SA+A++FPL  E++  L S ASL  +V F+KGG LS RRN++L LK+
Sbjct: 181 -FNRNDAVLEEILSALALLFPLDGEAMSCLGSAASLHCMVRFLKGGDLSGRRNAVLALKE 240

Query: 241 IISSYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALL 300
           I+SS  +K   L EI+G+LE LVKLIKEPIC SS KASL   YH++ SS   +   +  +
Sbjct: 241 ILSSDERKVNALSEIEGSLEALVKLIKEPICPSSTKASLMAIYHVVNSSFPKETIVERFV 300

Query: 301 EMGFVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDL 360
           EMG VSLLLE LVDAERS+CE+ALG  DGIC S++GRE+ Y HALT+PV+VKKILRVSDL
Sbjct: 301 EMGLVSLLLEMLVDAERSICEKALGILDGICRSEKGREKPYDHALTIPVLVKKILRVSDL 360

Query: 361 ATELSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLK 420
           ATE SVSI+W+L K  +E+ EE G + +EALQVGAFQKLLLLLQVGC+E TKEKATELLK
Sbjct: 361 ATEFSVSIIWELCK--NEKREEGGVLVLEALQVGAFQKLLLLLQVGCAERTKEKATELLK 420

Query: 421 LLNNLHRDSLECIDSLDFKGIKRPF 443
           LL NLHRD LECIDS+DFK +KRPF
Sbjct: 421 LL-NLHRDRLECIDSMDFKDLKRPF 441

BLAST of Carg27436 vs. TrEMBL
Match: tr|A0A2P4JQ48|A0A2P4JQ48_QUESU (RING-type E3 ubiquitin transferase OS=Quercus suber OX=58331 GN=CFP56_45658 PE=4 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 4.7e-145
Identity = 269/446 (60.31%), Postives = 342/446 (76.68%), Query Frame = 0

Query: 1   MISSWRRRRATRRTA----RGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRAS 60
           MIS+WR+RRA RR A    RG   DM  +TIP+HFRCPISLD MKDPVTLSTGITYDR S
Sbjct: 1   MISTWRKRRAGRRAAKEQQRGENGDMELITIPNHFRCPISLDFMKDPVTLSTGITYDRDS 60

Query: 61  IETWIEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQ 120
           IE WIE GN +CP+TNQ  ++L+ IPNH IRKMIQDWCVEN++YG+ERIPTPR+PVS ++
Sbjct: 61  IEKWIEAGNHTCPVTNQVFRSLEPIPNHTIRKMIQDWCVENQSYGIERIPTPRIPVSSIE 120

Query: 121 IRDILEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQ 180
           + +IL KIT A++R D   C+  V KIK L KESER+K+CI+ + A  VIS+AF+ FS  
Sbjct: 121 VSEILSKITMASKREDQVWCQELVAKIKALVKESERDKRCIVSSKAGSVISAAFETFSIA 180

Query: 181 EPLEKSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILK 240
              +K++ +LEE++SA+ ++ P+ EE+   L S ASL  +V F+KGG LS RR++ L L 
Sbjct: 181 PSFDKNVAVLEEILSALTLLSPIDEEAKCYLGSAASLHCMVWFLKGGDLSARRSAALALN 240

Query: 241 QIISSYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKAL 300
           + +SS  +    L EI+GALE LVKLIKEPIC ++ KASL + YH++ SS   +   +  
Sbjct: 241 EAVSSNERVRDALAEIEGALEALVKLIKEPICPTATKASLMVVYHMVNSSFPNETIIERF 300

Query: 301 LEMGFVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSD 360
           ++MG VSLLLE LVD E+ +CE ALG  DG+C S +GRE+A+ +AL+MPV+VKK+LRVSD
Sbjct: 301 VDMGLVSLLLEILVDTEKHICEEALGVLDGLCSSDKGREKAFNNALSMPVLVKKVLRVSD 360

Query: 361 LATELSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELL 420
           LATE SVSI+WKL K  +E++EE G V +EALQVGAFQKLLLL+QVGC+E TKEKATELL
Sbjct: 361 LATEFSVSIIWKLCK--NEKKEEGGGVLIEALQVGAFQKLLLLIQVGCAEKTKEKATELL 420

Query: 421 KLLNNLHRDSLECIDSLDFKGIKRPF 443
           K L NLHRD LECIDS+DFK +KRPF
Sbjct: 421 KWL-NLHRDRLECIDSMDFKELKRPF 443

BLAST of Carg27436 vs. TrEMBL
Match: tr|F6HFB5|F6HFB5_VITVI (RING-type E3 ubiquitin transferase OS=Vitis vinifera OX=29760 GN=VIT_01s0011g06140 PE=4 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 3.4e-143
Identity = 274/449 (61.02%), Postives = 341/449 (75.95%), Query Frame = 0

Query: 1   MISSWRRRRATRRTAR-------GTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYD 60
           MISSWRRRRA RR A+         GS   ELT P+HFRCPISLD+MKDPVTLSTGITYD
Sbjct: 1   MISSWRRRRAGRRAAKLQQHAEDDIGS--MELTTPNHFRCPISLDLMKDPVTLSTGITYD 60

Query: 61  RASIETWIEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVS 120
           R SIE WIE GN +CPITNQ L++L+ IPNH IRKMIQDWCVEN+++G+ERIPTPR+P+S
Sbjct: 61  RESIEMWIEAGNRTCPITNQVLRSLEPIPNHTIRKMIQDWCVENRSFGIERIPTPRIPLS 120

Query: 121 PVQIRDILEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAF 180
            V++ D+L K   A RR D  GC+  V K+K   KESERNK+CI+ NGA+ V+S+AF+AF
Sbjct: 121 SVEVTDMLSKFKMAYRREDEAGCRELVAKMKSKGKESERNKRCIVINGAAGVLSAAFEAF 180

Query: 181 SKQEPLEKSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSIL 240
           S     +K + +LE++++A+  M PL  E+   L S ASL  LV  +K G LS R N++ 
Sbjct: 181 SSAS-FDKYVAVLEDILAALTWMSPLDGEAKSYLSSAASLNCLVWLLKSGDLSARGNAVS 240

Query: 241 ILKQIISSYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFT 300
            LK+++SS  +K   L EI+GA E LVKL+KEPIC ++ K SL + +H+++SS + +   
Sbjct: 241 TLKELLSSDKRKVYALSEIEGAKEALVKLVKEPICPTATKTSLVVIFHMVSSSPSNEDTK 300

Query: 301 KALLEMGFVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILR 360
              +EMG V LLLE LVD+E+SVCE+ALG  DGIC  +EGRE+AY HALTMPV+VKK+LR
Sbjct: 301 VRFVEMGLVELLLELLVDSEKSVCEKALGVLDGICGCEEGREKAYGHALTMPVLVKKLLR 360

Query: 361 VSDLATELSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKAT 420
           VSDLATE SVSI+WKL K+   E+ EDG V VEALQVGAFQKLLLLLQVGCS+ TKEKAT
Sbjct: 361 VSDLATEFSVSILWKLSKN---EKREDGSVLVEALQVGAFQKLLLLLQVGCSDRTKEKAT 420

Query: 421 ELLKLLNNLHRDSLECIDSLDFKGIKRPF 443
           ELLKLL N+HR+ LECIDS+DFK +KRPF
Sbjct: 421 ELLKLL-NIHRERLECIDSMDFKDLKRPF 442

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023512745.19.0e-24198.64U-box domain-containing protein 21-like [Cucurbita pepo subsp. pepo][more]
XP_022942705.13.2e-23897.96U-box domain-containing protein 21-like [Cucurbita moschata][more]
XP_022995728.11.8e-23395.70U-box domain-containing protein 21-like [Cucurbita maxima][more]
XP_004142305.11.7e-20281.80PREDICTED: U-box domain-containing protein 21-like [Cucumis sativus] >KGN49607.1... [more]
XP_008465536.16.3e-20281.35PREDICTED: U-box domain-containing protein 21-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT5G37490.18.5e-10047.14ARM repeat superfamily protein[more]
AT1G66160.12.6e-8541.45CYS, MET, PRO, and GLY protein 1[more]
AT3G02840.19.2e-6240.17ARM repeat superfamily protein[more]
AT1G49780.12.2e-5534.37plant U-box 26[more]
AT3G19380.17.0e-5433.73plant U-box 25[more]
Match NameE-valueIdentityDescription
sp|Q5PNY6|PUB21_ARATH1.5e-9847.14U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2... [more]
sp|Q9C8D1|PUB20_ARATH4.8e-8441.45U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=2... [more]
sp|Q9FXA4|PUB26_ARATH3.9e-5434.37U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
sp|Q9LT79|PUB25_ARATH1.3e-5233.73U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
sp|Q84TG3|PUB23_ARATH4.5e-5032.60E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0KMI8|A0A0A0KMI8_CUCSA1.1e-20281.80RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G023870 PE... [more]
tr|A0A1S3CPH4|A0A1S3CPH4_CUCME4.2e-20281.35RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103503164 PE=4 ... [more]
tr|A0A2I4G200|A0A2I4G200_9ROSI9.8e-15163.82RING-type E3 ubiquitin transferase OS=Juglans regia OX=51240 GN=LOC109003997 PE=... [more]
tr|A0A2P4JQ48|A0A2P4JQ48_QUESU4.7e-14560.31RING-type E3 ubiquitin transferase OS=Quercus suber OX=58331 GN=CFP56_45658 PE=4... [more]
tr|F6HFB5|F6HFB5_VITVI3.4e-14361.02RING-type E3 ubiquitin transferase OS=Vitis vinifera OX=29760 GN=VIT_01s0011g061... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005488binding
GO:0004842ubiquitin-protein transferase activity
Vocabulary: Biological Process
TermDefinition
GO:0016567protein ubiquitination
Vocabulary: INTERPRO
TermDefinition
IPR016024ARM-type_fold
IPR011989ARM-like
IPR013083Znf_RING/FYVE/PHD
IPR003613Ubox_domain
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005575 cellular_component
molecular_function GO:0005488 binding
molecular_function GO:0004842 ubiquitin-protein transferase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27436-RACarg27436-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 30..93
e-value: 4.2E-28
score: 109.4
IPR003613U box domainPFAMPF04564U-boxcoord: 27..98
e-value: 2.6E-19
score: 69.0
IPR003613U box domainPROSITEPS51698U_BOXcoord: 26..100
score: 40.011
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 5..104
e-value: 2.3E-28
score: 100.1
IPR011989Armadillo-like helicalGENE3DG3DSA:1.25.10.10coord: 109..435
e-value: 8.4E-13
score: 49.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 1..442
NoneNo IPR availablePANTHERPTHR22849:SF102SUBFAMILY NOT NAMEDcoord: 1..442
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 31..73
e-value: 9.01481E-24
score: 92.2966
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 18..103
IPR016024Armadillo-type foldSUPERFAMILYSSF48371ARM repeatcoord: 388..420
coord: 118..351