Carg26824 (gene) Silver-seed gourd

NameCarg26824
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionCytoplasmic tRNA 2-thiolation protein 1
LocationCucurbita_argyrosperma_scaffold_473 : 12301 .. 13611 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGTTGAAGTGGATTCTGAATTCCGCTTTCACTCAAGTTCTAGGGCTTACAGAGAAGCAACAACACAATCAGAACGAGGCGGGAGAAGGAATCAAGAACGTGGAGCAAGTGAAGGAGTCGGAAGATTGTTTTTATGGAGGTTTGAATTTTGAAAGTGGGTTTCAAATGCCTCTCCATTATCCCAGGTACACGAAGGCGGATTATGAGAAAATGGAAGACGGTAAATTGGATTTGCTTCTGAAACAATATGGACTCCGTTTTGAAGGTACCCTTGAGGAGAAAAGGGCATTTGCTATTGGCACGTTCTTGTGGCCTGACCAACTTTGAGTTTGCCCACCAGGTGTTTGATGAAAGTCCCCAATGAAAAAACTAGCTAACTTTTGATGTTTGCTGTTTGTATAGCTACTTTATGGATGTGATGTTAGATTAAGATGCAGAGATATTAGTAGCTGTGTCTTTGTTTGGCTGTATGTACTGATCTTGGTGGGATTTTAGTGCATCTTGTAAAAAACTAGTTACATATAATGGTATATTAATCACCCTTTTCTACTGAGTCCATGATTTTTCTTTGCAAAACAAGTATAATCTAGTAAAAGAAACAGGGAGAATTTGGTGTGTTCTTCAAGAAAACAAGGAAGAGAAGCTATGAATGTAGTGTCCATTCATAGTCTCTCACATAATTGTACAACATCTCTGATTGTTGTTATTATCTTGGCTGGGTTCCTCAGGGGCTGGATTACCAGGTGGCCCGCCCGGCCCTATCATTGGCCATGGGACAGGCTGCGGCTGTGGCTGTGGCAGCATGAACTGCCAAGGGTATGGCTGCGGCGGCAGCGGTGGTTGTTGGGGGTAAGATGTGTCAAACCAGCGAGATTGTTCTTGGGAATTCTGATAAGAATGATGAATGGGATCATCTCCATAAGGGTAATATCTTCTATCTCCAGCTTCACCGTCAAATCTTATACTTTTAACCTCTGCATGTTTGCCTGACTTTTCAATCACTTTCAGGAATGTTCTTGGATTCACTCTTCCACAGATTCTGACTGACCCATCATCTGAATCCATTGTGATGGTGTAAATACCTGGGAATCAAAAACAATGAAGAACAATGAAGAACAATGAAGAACATGAAGAACAATGAGGTAAAGAGAGAAACCATAGATGTTCTGTAGAATCTCTTGGACTTTTGCTGTGCATGCTTCACATTGAATACTTGCACTAAGAACACAACTCTGCAACAAAGTGAAAATGTTTTAGGTTTAGAAGAACATGAAAAGCAAAGAAAAGGGCGTCTGTTTATGGAGTGAA

mRNA sequence

ATGGGGTTGAAGTGGATTCTGAATTCCGCTTTCACTCAAGTTCTAGGGCTTACAGAGAAGCAACAACACAATCAGAACGAGGCGGGAGAAGGAATCAAGAACGTGGAGCAAGTGAAGGAGTCGGAAGATTGTTTTTATGGAGGTTTGAATTTTGAAAGTGGGTTTCAAATGCCTCTCCATTATCCCAGGTACACGAAGGCGGATTATGAGAAAATGGAAGACGGTAAATTGGATTTGCTTCTGAAACAATATGGACTCCGTTTTGAAGGGGCTGGATTACCAGGTGGCCCGCCCGGCCCTATCATTGGCCATGGGACAGGCTGCGGCTGTGGCTGTGGCAGCATGAACTGCCAAGGGTATGGCTGCGGCGGCAGCGGTGGTTGTTGGGGGAATGTTCTTGGATTCACTCTTCCACAGATTCTGACTGACCCATCATCTGAATCCATTGTGATGGTGTAAATACCTGGGAATCAAAAACAATGAAGAACAATGAAGAACAATGAAGAACATGAAGAACAATGAGGTAAAGAGAGAAACCATAGATGTTCTGTAGAATCTCTTGGACTTTTGCTGTGCATGCTTCACATTGAATACTTGCACTAAGAACACAACTCTGCAACAAAGTGAAAATGTTTTAGGTTTAGAAGAACATGAAAAGCAAAGAAAAGGGCGTCTGTTTATGGAGTGAA

Coding sequence (CDS)

ATGGGGTTGAAGTGGATTCTGAATTCCGCTTTCACTCAAGTTCTAGGGCTTACAGAGAAGCAACAACACAATCAGAACGAGGCGGGAGAAGGAATCAAGAACGTGGAGCAAGTGAAGGAGTCGGAAGATTGTTTTTATGGAGGTTTGAATTTTGAAAGTGGGTTTCAAATGCCTCTCCATTATCCCAGGTACACGAAGGCGGATTATGAGAAAATGGAAGACGGTAAATTGGATTTGCTTCTGAAACAATATGGACTCCGTTTTGAAGGGGCTGGATTACCAGGTGGCCCGCCCGGCCCTATCATTGGCCATGGGACAGGCTGCGGCTGTGGCTGTGGCAGCATGAACTGCCAAGGGTATGGCTGCGGCGGCAGCGGTGGTTGTTGGGGGAATGTTCTTGGATTCACTCTTCCACAGATTCTGACTGACCCATCATCTGAATCCATTGTGATGGTGTAA

Protein sequence

MGLKWILNSAFTQVLGLTEKQQHNQNEAGEGIKNVEQVKESEDCFYGGLNFESGFQMPLHYPRYTKADYEKMEDGKLDLLLKQYGLRFEGAGLPGGPPGPIIGHGTGCGCGCGSMNCQGYGCGGSGGCWGNVLGFTLPQILTDPSSESIVMV
BLAST of Carg26824 vs. NCBI nr
Match: XP_022932392.1 (uncharacterized protein LOC111438758 [Cucurbita moschata])

HSP 1 Score: 188.3 bits (477), Expect = 1.9e-44
Identity = 89/90 (98.89%), Postives = 89/90 (98.89%), Query Frame = 0

Query: 1  MGLKWILNSAFTQVLGLTEKQQHNQNEAGEGIKNVEQVKESEDCFYGGLNFESGFQMPLH 60
          MGLKWILNSAFTQVLGLTEKQQHNQNEAGEG KNVEQVKESEDCFYGGLNFESGFQMPLH
Sbjct: 1  MGLKWILNSAFTQVLGLTEKQQHNQNEAGEGTKNVEQVKESEDCFYGGLNFESGFQMPLH 60

Query: 61 YPRYTKADYEKMEDGKLDLLLKQYGLRFEG 91
          YPRYTKADYEKMEDGKLDLLLKQYGLRFEG
Sbjct: 61 YPRYTKADYEKMEDGKLDLLLKQYGLRFEG 90

BLAST of Carg26824 vs. NCBI nr
Match: XP_022965232.1 (uncharacterized protein LOC111465146 [Cucurbita maxima])

HSP 1 Score: 182.6 bits (462), Expect = 1.0e-42
Identity = 86/90 (95.56%), Postives = 88/90 (97.78%), Query Frame = 0

Query: 1  MGLKWILNSAFTQVLGLTEKQQHNQNEAGEGIKNVEQVKESEDCFYGGLNFESGFQMPLH 60
          MGLKWILNSAFTQVLGLTEKQ  NQNEAGEGIKNVEQVKE+EDCFYGGLNFESGFQMPLH
Sbjct: 1  MGLKWILNSAFTQVLGLTEKQHRNQNEAGEGIKNVEQVKEAEDCFYGGLNFESGFQMPLH 60

Query: 61 YPRYTKADYEKMEDGKLDLLLKQYGLRFEG 91
          YPRYTKADYEKME+GKLDLLLKQYGLRFEG
Sbjct: 61 YPRYTKADYEKMEEGKLDLLLKQYGLRFEG 90

BLAST of Carg26824 vs. NCBI nr
Match: XP_023553399.1 (uncharacterized protein LOC111810825 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 181.0 bits (458), Expect = 3.0e-42
Identity = 85/90 (94.44%), Postives = 89/90 (98.89%), Query Frame = 0

Query: 1  MGLKWILNSAFTQVLGLTEKQQHNQNEAGEGIKNVEQVKESEDCFYGGLNFESGFQMPLH 60
          MGLKWILNSAFTQVLGLTEKQQHN+NEA EGIKNVEQVKESE+CFYGGLNFESGFQMPLH
Sbjct: 1  MGLKWILNSAFTQVLGLTEKQQHNRNEAREGIKNVEQVKESENCFYGGLNFESGFQMPLH 60

Query: 61 YPRYTKADYEKMEDGKLDLLLKQYGLRFEG 91
          YPRYTKADYE+ME+GKLDLLLKQYGLRFEG
Sbjct: 61 YPRYTKADYERMEEGKLDLLLKQYGLRFEG 90

BLAST of Carg26824 vs. NCBI nr
Match: XP_011650470.1 (PREDICTED: uncharacterized protein LOC105434788 [Cucumis sativus] >KGN56014.1 hypothetical protein Csa_3G047750 [Cucumis sativus])

HSP 1 Score: 161.8 bits (408), Expect = 1.9e-36
Identity = 80/92 (86.96%), Postives = 84/92 (91.30%), Query Frame = 0

Query: 1  MGLKWILNSAFTQVLGLTEKQQHNQNEA-GEGIKNVEQVKESEDCFYGG-LNFESGFQMP 60
          MGLKWILNSAFTQVLGLTEKQQ NQ EA GEGIKNVEQVKE  DCFYGG LNF+SGFQMP
Sbjct: 1  MGLKWILNSAFTQVLGLTEKQQQNQKEAGGEGIKNVEQVKEGRDCFYGGDLNFQSGFQMP 60

Query: 61 LHYPRYTKADYEKMEDGKLDLLLKQYGLRFEG 91
          LHYPRYTK DYE+ME+GKLDLLLKQYGL F+G
Sbjct: 61 LHYPRYTKRDYEEMEEGKLDLLLKQYGLCFDG 92

BLAST of Carg26824 vs. NCBI nr
Match: XP_008449025.1 (PREDICTED: uncharacterized protein LOC103491021 [Cucumis melo])

HSP 1 Score: 160.2 bits (404), Expect = 5.4e-36
Identity = 80/92 (86.96%), Postives = 83/92 (90.22%), Query Frame = 0

Query: 1  MGLKWILNSAFTQVLGLTEKQQHNQNEA-GEGIKNVEQVKESEDCFY-GGLNFESGFQMP 60
          MGLKWILNSAFTQVLGLTEK Q NQ EA GEGIKNVEQVKE  DCFY GGLNFESGFQMP
Sbjct: 1  MGLKWILNSAFTQVLGLTEKLQQNQKEAGGEGIKNVEQVKEGRDCFYGGGLNFESGFQMP 60

Query: 61 LHYPRYTKADYEKMEDGKLDLLLKQYGLRFEG 91
          LHYPRYTK DYE+ME+GKLDLLLKQYGL F+G
Sbjct: 61 LHYPRYTKRDYEEMEEGKLDLLLKQYGLCFDG 92

BLAST of Carg26824 vs. TAIR10
Match: AT5G55620.1 (unknown protein)

HSP 1 Score: 80.5 bits (197), Expect = 9.9e-16
Identity = 41/91 (45.05%), Postives = 56/91 (61.54%), Query Frame = 0

Query: 1  MGLKWILNSAFTQVLGLTEKQQHNQNEAGEGIKNVEQVKESEDCFYGGLNFESGFQMPLH 60
          M +K +  SAFTQ+ G +       N      K ++ +K+ E        F SGFQ+PLH
Sbjct: 1  MEMKLLFTSAFTQMFGYSNHMDQASNCQSTRNKIIKMMKKEE--------FPSGFQVPLH 60

Query: 61 YPRYTKADYEKMEDGKLDLLLKQYGLRFEGA 92
          YP+Y+K+DYE M+D +LDLLLKQYG  FEG+
Sbjct: 61 YPKYSKSDYEVMDDLRLDLLLKQYGFSFEGS 83

BLAST of Carg26824 vs. TAIR10
Match: AT3G09950.1 (unknown protein)

HSP 1 Score: 63.2 bits (152), Expect = 1.6e-10
Identity = 26/34 (76.47%), Postives = 31/34 (91.18%), Query Frame = 0

Query: 53 SGFQMPLHYPRYTKADYEKMEDGKLDLLLKQYGL 87
          SGF+MPLHYPRYTK DYE+ME+ +LDLLL +YGL
Sbjct: 34 SGFKMPLHYPRYTKEDYEEMEEWRLDLLLSEYGL 67

BLAST of Carg26824 vs. TAIR10
Match: AT5G41761.1 (unknown protein)

HSP 1 Score: 58.9 bits (141), Expect = 3.1e-09
Identity = 31/65 (47.69%), Postives = 43/65 (66.15%), Query Frame = 0

Query: 23 HNQ-NEAGEGIKNVEQVKESEDCFYGGLNFESGFQMPLHYPRYTKADYEKMEDGKLDLLL 82
          HN+ ++ GE  +N    K + D      N  S FQ+PLHYP+YTK+DYEKM + +LD LL
Sbjct: 15 HNRSHQDGEDQRNETATKINHD---KPQNQSSSFQIPLHYPKYTKSDYEKMPEWQLDRLL 74

Query: 83 KQYGL 87
          ++YGL
Sbjct: 75 REYGL 76

BLAST of Carg26824 vs. TAIR10
Match: AT3G55570.1 (unknown protein)

HSP 1 Score: 50.8 bits (120), Expect = 8.4e-07
Identity = 22/38 (57.89%), Postives = 27/38 (71.05%), Query Frame = 0

Query: 53 SGFQMPLHYPRYTKADYEKMEDGKLDLLLKQYGLRFEG 91
          S F+MPLHYPRY+K DY+ M + KLD +L  YGL   G
Sbjct: 30 SVFRMPLHYPRYSKEDYQDMPEWKLDRVLADYGLSTYG 67

BLAST of Carg26824 vs. TAIR10
Match: AT3G11405.1 (unknown protein)

HSP 1 Score: 47.0 bits (110), Expect = 1.2e-05
Identity = 21/39 (53.85%), Postives = 25/39 (64.10%), Query Frame = 0

Query: 48 GLNFESGFQMPLHYPRYTKADYEKMEDGKLDLLLKQYGL 87
          G +  S FQMPL YP Y K  Y+ M + +LD LLK YGL
Sbjct: 50 GSSSPSSFQMPLQYPNYAKEQYDIMSEEELDRLLKLYGL 88

BLAST of Carg26824 vs. TrEMBL
Match: tr|A0A0A0L4U1|A0A0A0L4U1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G047750 PE=4 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.2e-36
Identity = 80/92 (86.96%), Postives = 84/92 (91.30%), Query Frame = 0

Query: 1  MGLKWILNSAFTQVLGLTEKQQHNQNEA-GEGIKNVEQVKESEDCFYGG-LNFESGFQMP 60
          MGLKWILNSAFTQVLGLTEKQQ NQ EA GEGIKNVEQVKE  DCFYGG LNF+SGFQMP
Sbjct: 1  MGLKWILNSAFTQVLGLTEKQQQNQKEAGGEGIKNVEQVKEGRDCFYGGDLNFQSGFQMP 60

Query: 61 LHYPRYTKADYEKMEDGKLDLLLKQYGLRFEG 91
          LHYPRYTK DYE+ME+GKLDLLLKQYGL F+G
Sbjct: 61 LHYPRYTKRDYEEMEEGKLDLLLKQYGLCFDG 92

BLAST of Carg26824 vs. TrEMBL
Match: tr|A0A1S3BM13|A0A1S3BM13_CUCME (uncharacterized protein LOC103491021 OS=Cucumis melo OX=3656 GN=LOC103491021 PE=4 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 3.6e-36
Identity = 80/92 (86.96%), Postives = 83/92 (90.22%), Query Frame = 0

Query: 1  MGLKWILNSAFTQVLGLTEKQQHNQNEA-GEGIKNVEQVKESEDCFY-GGLNFESGFQMP 60
          MGLKWILNSAFTQVLGLTEK Q NQ EA GEGIKNVEQVKE  DCFY GGLNFESGFQMP
Sbjct: 1  MGLKWILNSAFTQVLGLTEKLQQNQKEAGGEGIKNVEQVKEGRDCFYGGGLNFESGFQMP 60

Query: 61 LHYPRYTKADYEKMEDGKLDLLLKQYGLRFEG 91
          LHYPRYTK DYE+ME+GKLDLLLKQYGL F+G
Sbjct: 61 LHYPRYTKRDYEEMEEGKLDLLLKQYGLCFDG 92

BLAST of Carg26824 vs. TrEMBL
Match: tr|A0A2C9UDL3|A0A2C9UDL3_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_15G061400 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.0e-14
Identity = 51/106 (48.11%), Postives = 65/106 (61.32%), Query Frame = 0

Query: 1   MGLKWILNSAFTQVLGLTEK----QQHNQNEA-----GEGIKNV-EQVKESEDCFYGGL- 60
           M LKW LNSAFTQV G T+     Q   +++       +G+  V E V+ S D   G + 
Sbjct: 1   MALKWFLNSAFTQVFGHTDSSVTVQTRQRSDVVACPNNQGLLQVHECVRSSRDVKEGSVT 60

Query: 61  -----NFESGFQMPLHYPRYTKADYEKMEDGKLDLLLKQYGLRFEG 91
                 + +GFQ+PLHYPRYT ADYEKME+ KLD+LL +YGL FEG
Sbjct: 61  RTRKQEYPNGFQVPLHYPRYTAADYEKMEEWKLDMLLNEYGLSFEG 106

BLAST of Carg26824 vs. TrEMBL
Match: tr|A0A2P2MI74|A0A2P2MI74_RHIMU (Uncharacterized protein MANES_15G061400 OS=Rhizophora mucronata OX=61149 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 2.3e-14
Identity = 50/101 (49.50%), Postives = 61/101 (60.40%), Query Frame = 0

Query: 1   MGLKWILNSAFTQVLGLTEK---QQHNQNEAGEGIKNVEQVKESEDCFYGG--------L 60
           M L W LNSAFTQVLG T+    QQH+   +    +   Q +  +D    G         
Sbjct: 23  MALNWFLNSAFTQVLGRTDSVGMQQHSNVISCPTDQECVQSQSLKDVAEEGGAMIVMRKE 82

Query: 61  NFESGFQMPLHYPRYTKADYEKMEDGKLDLLLKQYGLRFEG 91
            + +GFQ+PLHYPRY KADYEKMED KLDLLL +YGL F+G
Sbjct: 83  EYPNGFQVPLHYPRYKKADYEKMEDWKLDLLLNEYGLGFKG 123

BLAST of Carg26824 vs. TrEMBL
Match: tr|W9R1F4|W9R1F4_9ROSA (Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_018236 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 2.3e-14
Identity = 50/99 (50.51%), Postives = 60/99 (60.61%), Query Frame = 0

Query: 1  MGLKWILNSAFTQVLGLTEKQQHNQNEA---GEGIK-----NVEQVKESEDCFYGGLNFE 60
          M LKW LNS FT VLG      H QN     G GIK     NV  V   ++       + 
Sbjct: 1  MALKWFLNSTFTHVLG------HTQNVGMIEGSGIKEGKERNVSMVSICKE------EYP 60

Query: 61 SGFQMPLHYPRYTKADYEKMEDGKLDLLLKQYGLRFEGA 92
          +GFQMPLHYP+Y KADYEKME+ KLDLLLK+YG + +G+
Sbjct: 61 NGFQMPLHYPKYNKADYEKMEECKLDLLLKEYGFKVKGS 87

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022932392.11.9e-4498.89uncharacterized protein LOC111438758 [Cucurbita moschata][more]
XP_022965232.11.0e-4295.56uncharacterized protein LOC111465146 [Cucurbita maxima][more]
XP_023553399.13.0e-4294.44uncharacterized protein LOC111810825 [Cucurbita pepo subsp. pepo][more]
XP_011650470.11.9e-3686.96PREDICTED: uncharacterized protein LOC105434788 [Cucumis sativus] >KGN56014.1 hy... [more]
XP_008449025.15.4e-3686.96PREDICTED: uncharacterized protein LOC103491021 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT5G55620.19.9e-1645.05unknown protein[more]
AT3G09950.11.6e-1076.47unknown protein[more]
AT5G41761.13.1e-0947.69unknown protein[more]
AT3G55570.18.4e-0757.89unknown protein[more]
AT3G11405.11.2e-0553.85unknown protein[more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
tr|A0A0A0L4U1|A0A0A0L4U1_CUCSA1.2e-3686.96Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G047750 PE=4 SV=1[more]
tr|A0A1S3BM13|A0A1S3BM13_CUCME3.6e-3686.96uncharacterized protein LOC103491021 OS=Cucumis melo OX=3656 GN=LOC103491021 PE=... [more]
tr|A0A2C9UDL3|A0A2C9UDL3_MANES1.0e-1448.11Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_15G061400 PE=4 SV=... [more]
tr|A0A2P2MI74|A0A2P2MI74_RHIMU2.3e-1449.50Uncharacterized protein MANES_15G061400 OS=Rhizophora mucronata OX=61149 PE=4 SV... [more]
tr|W9R1F4|W9R1F4_9ROSA2.3e-1450.51Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_018236 PE=4 SV=1[more]
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg26824-RACarg26824-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33513FAMILY NOT NAMEDcoord: 1..91
NoneNo IPR availablePANTHERPTHR33513:SF2SUBFAMILY NOT NAMEDcoord: 1..91

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Carg26824Watermelon (Charleston Gray)carwcgB0004
Carg26824Watermelon (97103) v1carwmB0006