Carg26040 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg26040
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	EF hand calcium-binding family protein
Location	Cucurbita_argyrosperma_scaffold_258 : 17550 .. 17972 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCCCAATTCTCGCGCTACCAGAGAGTGTTCAACAGTATAGACTCCGACGGCGACGGCAAGGTCTCCTCTCAAGATTTGCTTACCCGGTTCGCGGCGGTGACAGGGGAGGAGCTCACGGAGGAGGAGGCGGCAGCGGTGGTGGCGGCCTACGATTCCGACGACGACGGGCTATTGGAGATTGCGGAGTTTGAGAGGTTGGTGGAGGATGGGGATGGAGAGGGGAAGGAGATAAAGGAGGCTTTCAAGATGTATGAAATGGAGGGATTGGGTGTGATTACGGCGGTGAGCCTGCGGCGGATGCTGAGCCAGCTGGGGCAATCGAGGAGCATCGGGGATTGCAACAGAATGATCGCTAAGTTTGATGTCAATGGCGATGGAGTTCTCTGTTTTGATGAGTTCAGGATTATGATGTTGCTCTGA

mRNA sequence

ATGCCCCAATTCTCGCGCTACCAGAGAGTGTTCAACAGTATAGACTCCGACGGCGACGGCAAGGTCTCCTCTCAAGATTTGCTTACCCGGTTCGCGGCGGTGACAGGGGAGGAGCTCACGGAGGAGGAGGCGGCAGCGGTGGTGGCGGCCTACGATTCCGACGACGACGGGCTATTGGAGATTGCGGAGTTTGAGAGGTTGGTGGAGGATGGGGATGGAGAGGGGAAGGAGATAAAGGAGGCTTTCAAGATGTATGAAATGGAGGGATTGGGTGTGATTACGGCGGTGAGCCTGCGGCGGATGCTGAGCCAGCTGGGGCAATCGAGGAGCATCGGGGATTGCAACAGAATGATCGCTAAGTTTGATGTCAATGGCGATGGAGTTCTCTGTTTTGATGAGTTCAGGATTATGATGTTGCTCTGA

Coding sequence (CDS)

ATGCCCCAATTCTCGCGCTACCAGAGAGTGTTCAACAGTATAGACTCCGACGGCGACGGCAAGGTCTCCTCTCAAGATTTGCTTACCCGGTTCGCGGCGGTGACAGGGGAGGAGCTCACGGAGGAGGAGGCGGCAGCGGTGGTGGCGGCCTACGATTCCGACGACGACGGGCTATTGGAGATTGCGGAGTTTGAGAGGTTGGTGGAGGATGGGGATGGAGAGGGGAAGGAGATAAAGGAGGCTTTCAAGATGTATGAAATGGAGGGATTGGGTGTGATTACGGCGGTGAGCCTGCGGCGGATGCTGAGCCAGCTGGGGCAATCGAGGAGCATCGGGGATTGCAACAGAATGATCGCTAAGTTTGATGTCAATGGCGATGGAGTTCTCTGTTTTGATGAGTTCAGGATTATGATGTTGCTCTGA

Protein sequence

MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLEIAEFERLVEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAKFDVNGDGVLCFDEFRIMMLL

Homology

[Close]

BLAST of Carg26040 vs. NCBI nr
Match: XP_022962715.1 (probable calcium-binding protein CML31 [Cucurbita moschata])

HSP 1 Score: 238.8 bits (608), Expect = 1.1e-59
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0


Query: 1   MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLE 60
           MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLE
Sbjct: 1   MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLE 60

Query: 61  IAEFERLVEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAK 120
           IAEFERLVEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAK
Sbjct: 61  IAEFERLVEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAK 120

Query: 121 FDVNGDGVLCFDEFRIMMLL 141
           FDVNGDGVLCFDEFRIMMLL
Sbjct: 121 FDVNGDGVLCFDEFRIMMLL 140

[Close]

BLAST of Carg26040 vs. NCBI nr
Match: XP_022989992.1 (putative calcium-binding protein CML19 [Cucurbita maxima])

HSP 1 Score: 229.2 bits (583), Expect = 8.8e-57
Identity = 126/140 (90.00%), Postives = 128/140 (91.43%), Query Frame = 0


Query: 1   MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLE 60
           MPQFSRYQRVFNSIDSD DGKVSSQDLLTRFAAVTGE LT   XXXXXXX      GLLE
Sbjct: 1   MPQFSRYQRVFNSIDSDCDGKVSSQDLLTRFAAVTGEHLTEEEXXXXXXXYDSDDDGLLE 60

Query: 61  IAEFERLVEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAK 120
           IAEFER VE+GDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLG+SRSIGDCNRMIAK
Sbjct: 61  IAEFERFVEEGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGESRSIGDCNRMIAK 120

Query: 121 FDVNGDGVLCFDEFRIMMLL 141
           FDVNGDGVLCFDEFRIMMLL
Sbjct: 121 FDVNGDGVLCFDEFRIMMLL 140

[Close]

BLAST of Carg26040 vs. NCBI nr
Match: XP_023520938.1 (probable calcium-binding protein CML31 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 226.5 bits (576), Expect = 5.7e-56
Identity = 132/137 (96.35%), Postives = 135/137 (98.54%), Query Frame = 0


Query: 1   MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLE 60
           MPQFSRY+RVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLE
Sbjct: 1   MPQFSRYERVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLE 60

Query: 61  IAEFERLVEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAK 120
           IAEFER VE+GDGEGKEIKEAFKMYEMEGLGVITAVSLRRML QLG+SRSIGDCNRMIAK
Sbjct: 61  IAEFERFVEEGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLIQLGESRSIGDCNRMIAK 120

Query: 121 FDVNGDGVLCFDEFRIM 138
           FDVNGDGVLCFDEFRIM
Sbjct: 121 FDVNGDGVLCFDEFRIM 137

[Close]

BLAST of Carg26040 vs. NCBI nr
Match: XP_004150479.1 (PREDICTED: probable calcium-binding protein CML31 [Cucumis sativus] >KGN51513.1 hypothetical protein Csa_5G571560 [Cucumis sativus])

HSP 1 Score: 157.1 bits (396), Expect = 4.2e-35
Identity = 86/145 (59.31%), Postives = 103/145 (71.03%), Query Frame = 0


Query: 1   MPQFSRYQR-VFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLL 60
           M + S Y   +F +ID+D DGKVS  DLLTR  A+TG+ LT                GLL
Sbjct: 1   MDKLSPYHTLLFYNIDTDSDGKVSPHDLLTRLPALTGQVLTEAEAASAVAAFDSDGDGLL 60

Query: 61  EIAEFERLVE----DGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCN 120
            + EF RLVE     GD + +E++EAF+MYEMEGLGVIT VSLRRMLS+LG+SRS+GDC 
Sbjct: 61  GMEEFGRLVEGVVVGGDDDDREVREAFQMYEMEGLGVITPVSLRRMLSRLGESRSVGDCK 120

Query: 121 RMIAKFDVNGDGVLCFDEFRIMMLL 141
           RMIAKFD++GDGVLCFDEFR MMLL
Sbjct: 121 RMIAKFDLDGDGVLCFDEFRGMMLL 145

[Close]

BLAST of Carg26040 vs. NCBI nr
Match: XP_022928328.1 (putative calcium-binding protein CML19 [Cucurbita moschata])

HSP 1 Score: 132.1 bits (331), Expect = 1.5e-27
Identity = 69/140 (49.29%), Postives = 95/140 (67.86%), Query Frame = 0


Query: 1   MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLE 60
           M + S Y+RVF+  D+DGDGK+S  ++    AA++G E++                G L+
Sbjct: 1   MDKLSCYKRVFDHFDTDGDGKISPFEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60

Query: 61  IAEFERLVEDGDGEG--KEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMI 120
           + EFER V+DG+ E   KE++EAFKMYEM+G G+ITAVSLRR L +LG+  S+ DC  MI
Sbjct: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMDGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120

Query: 121 AKFDVNGDGVLCFDEFRIMM 139
           AKFD++ DGVL FDEF++MM
Sbjct: 121 AKFDLDSDGVLSFDEFKVMM 140

[Close]

BLAST of Carg26040 vs. TAIR10
Match: AT1G76650.1 (calmodulin-like 38)

HSP 1 Score: 94.4 bits (233), Expect = 6.1e-20
Identity = 53/133 (39.85%), Postives = 80/133 (60.15%), Query Frame = 0


Query: 8   QRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLEIAEFERL 67
           + VF+ +D++ DG++S ++L   F  + GE+L+                G+L+  EF +L
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTL-GEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQL 104

Query: 68  VEDGDGEGK--EIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAKFDVNG 127
           ++  D E K  E+K AF++Y  EG   IT  SL+ ML +LG+SR+  DC  MI+ FD+N 
Sbjct: 105 IKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 128 DGVLCFDEFRIMM 139
           DGVL FDEF +MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of Carg26040 vs. TAIR10
Match: AT5G42380.1 (calmodulin like 37)

HSP 1 Score: 92.8 bits (229), Expect = 1.8e-19
Identity = 56/133 (42.11%), Postives = 77/133 (57.89%), Query Frame = 0


Query: 10  VFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLEIAEFERLVE 69
           VF+ +D++ DGK+S ++L +   ++ G  L+                G ++  EF +L+E
Sbjct: 53  VFDYMDANSDGKISGEELQS-CVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLME 112

Query: 70  DGDGEG----KEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAKFDVNG 129
             DG      KE+KEAF MY MEG   ITA SLRR LS+LG+S ++  C  MI  FD N 
Sbjct: 113 GEDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQND 172

Query: 130 DGVLCFDEFRIMM 139
           DGVL FDEF +MM
Sbjct: 173 DGVLSFDEFVLMM 184

[Close]

BLAST of Carg26040 vs. TAIR10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 88.6 bits (218), Expect = 3.4e-18
Identity = 51/136 (37.50%), Postives = 79/136 (58.09%), Query Frame = 0


Query: 8   QRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLEIAEFERL 67
           + VF  +D++ DG++S+++L   F  + GE+++                G+L+I EF  L
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTL-GEQMSDEEAEAAVKLSDIDGDGMLDINEFALL 83

Query: 68  VEDGD-----GEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAKFD 127
           ++  D      + ++I EAF+MY  +G   IT  SL+ ML +LG+SR+  DC  MI  FD
Sbjct: 84  IKGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFD 143

Query: 128 VNGDGVLCFDEFRIMM 139
           +N DGVL FDEF +MM
Sbjct: 144 LNADGVLSFDEFALMM 158

[Close]

BLAST of Carg26040 vs. TAIR10
Match: AT3G50770.1 (calmodulin-like 41)

HSP 1 Score: 85.9 bits (211), Expect = 2.2e-17
Identity = 56/140 (40.00%), Postives = 83/140 (59.29%), Query Frame = 0


Query: 8   QRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLEIAEFERL 67
           ++VF+  DSDGDGK+S+ +L   F +V GE ++                G L   +F  L
Sbjct: 66  RQVFSHFDSDGDGKISAFELRHYFGSV-GEYISHEAAQEAINEVDTDADGSLGFEDFVGL 125

Query: 68  VE------DG--DGEGKEIKEAFKMYEME-GLGVITAVSLRRMLSQLGQSRSIGDCNRMI 127
           +       DG  DG+G E+K AF+M+E+E G G IT   L++ML +LG+SR+ G+C  MI
Sbjct: 126 MTRRDLYGDGEVDGDG-ELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMI 185

Query: 128 AKFDVNGDGVLCFDEFRIMM 139
             +D++G+G+L F EFR MM
Sbjct: 186 KFYDIDGNGILDFHEFRQMM 203

[Close]

BLAST of Carg26040 vs. TAIR10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 77.8 bits (190), Expect = 5.9e-15
Identity = 46/131 (35.11%), Postives = 71/131 (54.20%), Query Frame = 0


Query: 8   QRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLEIAEFERL 67
           ++VF+  DS+GDGK+S  +L   F A+ G   T                G + + EF  L
Sbjct: 25  KKVFDQFDSNGDGKISVLELGGVFKAM-GTSYTETELNRVLEEVDTDRDGYINLDEFSTL 84

Query: 68  VEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAKFDVNGDG 127
                    EI++AF +Y+ +  G+I+A  L ++L++LG S S+ DC RMI   D +GDG
Sbjct: 85  CR-SSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDADGDG 144

Query: 128 VLCFDEFRIMM 139
            + F+EF+ MM
Sbjct: 145 NVNFEEFQKMM 153

[Close]

BLAST of Carg26040 vs. Swiss-Prot
Match: sp|Q8RYJ8|CML19_ORYSJ (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.3e-22
Identity = 59/139 (42.45%), Postives = 86/139 (61.87%), Query Frame = 0


Query: 5   SRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLEIAEF 64
           + ++RVF++ D D DGK+S+ +L     A  GE+++                GLL+  EF
Sbjct: 6   AEFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEEF 65

Query: 65  ERL---VEDGDGEG--KEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIA 124
            +L   +E GD E   + + EAF+MYEMEG G IT  SL+RMLS+LG  + I +C  MI 
Sbjct: 66  TKLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQTMIC 125

Query: 125 KFDVNGDGVLCFDEFRIMM 139
           +FD++GDGV+ F+EF+IMM
Sbjct: 126 RFDLDGDGVISFEEFKIMM 144

[Close]

BLAST of Carg26040 vs. Swiss-Prot
Match: sp|Q8RYJ9|CML23_ORYSJ (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 6.2e-22
Identity = 59/144 (40.97%), Postives = 80/144 (55.56%), Query Frame = 0


Query: 7   YQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLEIAEFER 66
           ++RVF S D DGDGK+S+ +L     A  GE++                 GLL+  EF R
Sbjct: 7   FRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDEEEFVR 66

Query: 67  LVED-----------GDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCN 126
           LV +                + ++EAF MYEMEG G IT +SL+ MLS+LG    + +C 
Sbjct: 67  LVTEMXXXXXXXXXXXXXXXRCLREAFAMYEMEGRGCITPLSLKLMLSKLGTHLDVAECQ 126

Query: 127 RMIAKFDVNGDGVLCFDEFRIMML 140
            MI +FD+NGDGVL FDEF+ MM+
Sbjct: 127 AMICRFDMNGDGVLTFDEFKTMMM 150

[Close]

BLAST of Carg26040 vs. Swiss-Prot
Match: sp|Q8RYK0|CML31_ORYSJ (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 2.6e-20
Identity = 71/135 (52.59%), Postives = 88/135 (65.19%), Query Frame = 0


Query: 10  VFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLEIAEFERLV- 69
           +F + D DGDG++S+ +L        GEE++ XXXXXXXXXXXXXXX         RLV 
Sbjct: 15  LFATFDHDGDGRISAAELRLCMKTTLGEEVSDXXXXXXXXXXXXXXXXXXXXXXXXRLVQ 74

Query: 70  -----EDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAKFDV 129
                E+ +  G  ++EAF MYEMEG G IT  SLRRML +LG  + I DC  MI +FD+
Sbjct: 75  AAEVEEEDERRGTGLREAFGMYEMEGEGCITPTSLRRMLRRLGSDQDIDDCRAMICRFDL 134

Query: 130 NGDGVLCFDEFRIMM 139
           NGDGVL FDEF+IMM
Sbjct: 135 NGDGVLSFDEFKIMM 149

[Close]

BLAST of Carg26040 vs. Swiss-Prot
Match: sp|Q9SRE6|CML38_ARATH (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.1e-18
Identity = 53/133 (39.85%), Postives = 80/133 (60.15%), Query Frame = 0


Query: 8   QRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLEIAEFERL 67
           + VF+ +D++ DG++S ++L   F  + GE+L+                G+L+  EF +L
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTL-GEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQL 104

Query: 68  VEDGDGEGK--EIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAKFDVNG 127
           ++  D E K  E+K AF++Y  EG   IT  SL+ ML +LG+SR+  DC  MI+ FD+N 
Sbjct: 105 IKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 128 DGVLCFDEFRIMM 139
           DGVL FDEF +MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of Carg26040 vs. Swiss-Prot
Match: sp|Q9FIH9|CML37_ARATH (Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 3.2e-18
Identity = 56/133 (42.11%), Postives = 77/133 (57.89%), Query Frame = 0


Query: 10  VFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLEIAEFERLVE 69
           VF+ +D++ DGK+S ++L +   ++ G  L+                G ++  EF +L+E
Sbjct: 53  VFDYMDANSDGKISGEELQS-CVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLME 112

Query: 70  DGDGEG----KEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAKFDVNG 129
             DG      KE+KEAF MY MEG   ITA SLRR LS+LG+S ++  C  MI  FD N 
Sbjct: 113 GEDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQND 172

Query: 130 DGVLCFDEFRIMM 139
           DGVL FDEF +MM
Sbjct: 173 DGVLSFDEFVLMM 184

[Close]

BLAST of Carg26040 vs. TrEMBL
Match: tr|A0A0A0KPM3|A0A0A0KPM3_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G571560 PE=4 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 2.8e-35
Identity = 86/145 (59.31%), Postives = 103/145 (71.03%), Query Frame = 0


Query: 1   MPQFSRYQR-VFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLL 60
           M + S Y   +F +ID+D DGKVS  DLLTR  A+TG+ LT                GLL
Sbjct: 1   MDKLSPYHTLLFYNIDTDSDGKVSPHDLLTRLPALTGQVLTEAEAASAVAAFDSDGDGLL 60

Query: 61  EIAEFERLVE----DGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCN 120
            + EF RLVE     GD + +E++EAF+MYEMEGLGVIT VSLRRMLS+LG+SRS+GDC 
Sbjct: 61  GMEEFGRLVEGVVVGGDDDDREVREAFQMYEMEGLGVITPVSLRRMLSRLGESRSVGDCK 120

Query: 121 RMIAKFDVNGDGVLCFDEFRIMMLL 141
           RMIAKFD++GDGVLCFDEFR MMLL
Sbjct: 121 RMIAKFDLDGDGVLCFDEFRGMMLL 145

[Close]

BLAST of Carg26040 vs. TrEMBL
Match: tr|A0A067LG72|A0A067LG72_JATCU (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_10143 PE=4 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 3.1e-26
Identity = 68/141 (48.23%), Postives = 92/141 (65.25%), Query Frame = 0


Query: 1   MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLE 60
           M ++  Y+ VF+  D +GDGK+S+ +L     A+ G +L+                GLL+
Sbjct: 2   MTKYQEYELVFSHFDENGDGKISASELQNCVGAI-GRQLSLAEAVAAIEHSDLDGDGLLD 61

Query: 61  IAEFERLVEDGDGEGK--EIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMI 120
           + EF R ++ G+ E K  ++KEAFKMYEME  G IT  SL+RMLS+LGQS SI +C  MI
Sbjct: 62  LEEFVRFIDGGEEEEKVNDLKEAFKMYEMEESGCITPKSLKRMLSRLGQSSSIEECKTMI 121

Query: 121 AKFDVNGDGVLCFDEFRIMML 140
           AKFD+NGDGVL FDEF++MML
Sbjct: 122 AKFDLNGDGVLNFDEFKVMML 141

[Close]

BLAST of Carg26040 vs. TrEMBL
Match: tr|A0A1S3AUA4|A0A1S3AUA4_CUCME (probable calcium-binding protein CML31 OS=Cucumis melo OX=3656 GN=LOC103483041 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 4.1e-26
Identity = 75/144 (52.08%), Postives = 88/144 (61.11%), Query Frame = 0


Query: 1   MPQFSRYQR-VFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLL 60
           M + S Y   +F +ID+D DGKVS  DL TR  A+TG +LT                GLL
Sbjct: 1   MDKLSPYHTLLFYTIDTDSDGKVSPHDLFTRLPALTGLDLTEAEAASAVAAYDSDGDGLL 60

Query: 61  EIAEFERL---VEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNR 120
            + EF RL   V+D                 EGLGVIT VSLRRMLS+LG+SRS+GDC R
Sbjct: 61  GMEEFGRLVKGVDDXXXXXXXXXXXXXXXXXEGLGVITPVSLRRMLSRLGESRSVGDCKR 120

Query: 121 MIAKFDVNGDGVLCFDEFRIMMLL 141
           MIAKFD++GDGVLCFDEFR MMLL
Sbjct: 121 MIAKFDLDGDGVLCFDEFRGMMLL 144

[Close]

BLAST of Carg26040 vs. TrEMBL
Match: tr|A0A1S3UR21|A0A1S3UR21_VIGRR (putative calcium-binding protein CML19 OS=Vigna radiata var. radiata OX=3916 GN=LOC106768082 PE=4 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 6.9e-26
Identity = 67/141 (47.52%), Postives = 93/141 (65.96%), Query Frame = 0


Query: 1   MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLE 60
           M + S+Y RVFN  D +GDG++S+ +L     A+ G +++                GL+ 
Sbjct: 2   MEKVSQYVRVFNHFDENGDGRISASELRQCVEAI-GGKVSWAEAEALVEVLDSDKDGLVG 61

Query: 61  IAEFERLVEDGDGEG--KEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMI 120
           + +F R VE+G+ E   K++KEAFKMYEMEG G IT  SL+RML +LG+ +SI +C  MI
Sbjct: 62  LDDFVRFVEEGEEEEKVKDLKEAFKMYEMEGCGCITPKSLKRMLGRLGECKSIDECQTMI 121

Query: 121 AKFDVNGDGVLCFDEFRIMML 140
           A+FD+NGDGVL FDEFR+MML
Sbjct: 122 ARFDLNGDGVLSFDEFRVMML 141

[Close]

BLAST of Carg26040 vs. TrEMBL
Match: tr|A0A151QQW8|A0A151QQW8_CAJCA (Putative calcium-binding protein CML19 OS=Cajanus cajan OX=3821 GN=KK1_046572 PE=4 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.0e-25
Identity = 68/141 (48.23%), Postives = 91/141 (64.54%), Query Frame = 0


Query: 1   MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTXXXXXXXXXXXXXXXXGLLE 60
           M + S+Y RVFN  D +GDGK+S  +L     AV G EL+                GL+ 
Sbjct: 1   MDKMSQYVRVFNHFDENGDGKISPSELRQCVEAV-GGELSLEEAEAVVELLDSDKDGLVG 60

Query: 61  IAEFERLVEDGDGEG--KEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMI 120
           + +F R  E+G+ E   K+++EAFKMYEMEG G IT  SL+RML +LG+ +SI +C  MI
Sbjct: 61  LDDFVRFAEEGEEEEKVKDLREAFKMYEMEGCGCITPKSLKRMLGRLGECKSIDECKVMI 120

Query: 121 AKFDVNGDGVLCFDEFRIMML 140
           A+FD++GDGVL FDEFR+MML
Sbjct: 121 ARFDLDGDGVLSFDEFRVMML 140

The following BLAST results are available for this feature:

BLAST of Carg26040 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022962715.1	1.1e-59	100.00	probable calcium-binding protein CML31 [Cucurbita moschata]	[more]
XP_022989992.1	8.8e-57	90.00	putative calcium-binding protein CML19 [Cucurbita maxima]	[more]
XP_023520938.1	5.7e-56	96.35	probable calcium-binding protein CML31 [Cucurbita pepo subsp. pepo]	[more]
XP_004150479.1	4.2e-35	59.31	PREDICTED: probable calcium-binding protein CML31 [Cucumis sativus] >KGN51513.1 ...	[more]
XP_022928328.1	1.5e-27	49.29	putative calcium-binding protein CML19 [Cucurbita moschata]	[more]

BLAST of Carg26040 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G76650.1	6.1e-20	39.85	calmodulin-like 38	[more]
AT5G42380.1	1.8e-19	42.11	calmodulin like 37	[more]
AT1G76640.1	3.4e-18	37.50	Calcium-binding EF-hand family protein	[more]
AT3G50770.1	2.2e-17	40.00	calmodulin-like 41	[more]
AT1G18210.1	5.9e-15	35.11	Calcium-binding EF-hand family protein	[more]

BLAST of Carg26040 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q8RYJ8\|CML19_ORYSJ	1.3e-22	42.45	Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
sp\|Q8RYJ9\|CML23_ORYSJ	6.2e-22	40.97	Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
sp\|Q8RYK0\|CML31_ORYSJ	2.6e-20	52.59	Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
sp\|Q9SRE6\|CML38_ARATH	1.1e-18	39.85	Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=2 SV=1	[more]
sp\|Q9FIH9\|CML37_ARATH	3.2e-18	42.11	Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=2 SV=1	[more]

BLAST of Carg26040 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KPM3\|A0A0A0KPM3_CUCSA	2.8e-35	59.31	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G571560 PE=4 SV=1	[more]
tr\|A0A067LG72\|A0A067LG72_JATCU	3.1e-26	48.23	Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_10143 PE=4 SV=1	[more]
tr\|A0A1S3AUA4\|A0A1S3AUA4_CUCME	4.1e-26	52.08	probable calcium-binding protein CML31 OS=Cucumis melo OX=3656 GN=LOC103483041 P...	[more]
tr\|A0A1S3UR21\|A0A1S3UR21_VIGRR	6.9e-26	47.52	putative calcium-binding protein CML19 OS=Vigna radiata var. radiata OX=3916 GN=...	[more]
tr\|A0A151QQW8\|A0A151QQW8_CAJCA	2.0e-25	48.23	Putative calcium-binding protein CML19 OS=Cajanus cajan OX=3821 GN=KK1_046572 PE...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS
IPR002048	EF_hand_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg26040-RA	Carg26040-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 43..71 e-value: 68.0 score: 3.0 coord: 113..140 e-value: 0.4 score: 19.7 coord: 6..34 e-value: 0.13 score: 21.4 coord: 77..105 e-value: 3.9 score: 13.0
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 5..68 e-value: 1.1E-10 score: 41.8
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 114..138 e-value: 7.3E-7 score: 28.2
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 76..108 score: 7.484
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 2..37 score: 10.385
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 109..140 score: 10.748
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 39..74 score: 9.994
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 77..138 e-value: 8.69844E-11 score: 52.9353
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 1..73 e-value: 3.9E-13 score: 51.3
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 74..140 e-value: 8.4E-16 score: 59.9
None	No IPR available	PANTHER	PTHR43996:SF2	SUBFAMILY NOT NAMED	coord: 4..139
None	No IPR available	PANTHER	PTHR43996	FAMILY NOT NAMED	coord: 4..139
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 15..27
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 122..134
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 52..64
IPR011992	EF-hand domain pair	SUPERFAMILY	SSF47473	EF-hand	coord: 3..139

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg26040	Csa5G571560	Cucumber (Chinese Long) v2	carcuB0461
Carg26040	MELO3C005319	Melon (DHL92) v3.5.1	carmeB0436
Carg26040	CSPI05G19750	Wild cucumber (PI 183967)	carcpiB0465
Carg26040	Cucsa.280520	Cucumber (Gy14) v1	carcgyB0495
Carg26040	CmaCh04G020930	Cucurbita maxima (Rimu)	carcmaB0595
Carg26040	CmoCh04G021950	Cucurbita moschata (Rifu)	carcmoB0580
Carg26040	CsGy5G019250	Cucumber (Gy14) v2	carcgybB0437
Carg26040	MELO3C005319.2	Melon (DHL92) v3.6.1	carmedB0438
Carg26040	CsaV3_5G028580	Cucumber (Chinese Long) v3	carcucB0461
Carg26040	Bhi12G000763	Wax gourd	carwgoB0512

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None