Carg25856 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg25856
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase
Location	Cucurbita_argyrosperma_scaffold_337 : 8754 .. 10958 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAGATTCAAACTCCATGAAGCCCTAGGATGGAAGCGATAATTTGTACCACTTGTCATAATTATATTAGAAAGATATCTTCTGGAGGTGGGCGATCCGATCAGACGGCAATCACTGAAGTAGTTCAGCTGCTCAACTAAATGCTATGTACTCATTTTCTCATAAGATTCTCCAGATCTTCAATCGTTCTCCATTTCTCCATCAATGGCGGGATTTTTGGCAACTATTCGGCGCCTGTACCTCTCCATCTACAATTGGACTCTCTTCGTCGGATGGTAAGTTCTTCCCCCTTCTCAATCACTTCCCTCTTCATATTTCTACTCGTAATGTAAAATTTTGCTTGGGTGTTGAAGGGTTCAGGTGTTCTACTTCTCAGTCAAGCAATTGAAGGAATCTGGTCACCAACAGGTATATAATGCCGTCGAACGTCCTCTTCAGTTTGCTCAAACTGCAGCTGTTTTAGAGGTATTCCCTGATCGTTTCTGGTTTATGAGCTGATTATCGTGATAGAGCCTATTATGAAGTATTTTATAATGATTTCTCGCATTTTTCTACTTCGCAGATTCTCCATGGCCTCGTTGGTAAGCTATATTTGATCCGCTTACAAGTTTTGGTGTCGAAGATTTTCTTTCTTTCCCTGATTGTAGCTTAGAAACGAAGCTGAATCGTGGGTTTTGGTTTTTGCCTAATTAGGGTTTGTGAGATCCCCTGTAACAGCAACGTTACCACAGATAGGTTCTAGATTGTATTTGACTTGGGGCATCTTATGGAGTTTCCCTGAGGTTGAAACCCTTCTTTTTCATTCAATTAGCCATAAATTAGTGTTTATGTTTGTAATTTTAATTGATAAAACTGATTACTTCTTGGGTCTGGCAGGTTAGGAATCATGTACTTGTTTCCTCCTTGGTCATCAGCTGGTCTATCACAGAGGCAAGTACTTTTTTTTTACTGGAAACCTTAATTCCAAACTTAATTTACTCACTTTAGTTATTGATTATAACCTTTAACAAACCACAAGAGATGATCCAAGATTCTTTTTATAATGAATCTATGCTTATCGGCTTTATCGGCTTTATGTGTGTGTAACTCCAATGGTTGATGCTTTGTGATGGGCTTCTGATTTTTAGGAATTATGTACATTGTTAACTCAGTTTTTATTGTTAAGTTTGCCGACCATTGTATATTCTGTGAGATGATAATTTATTAGATCATCACTTCCTCATTCAAATTAGTAGATCTAGACTGTGTTTGACATGATTCGTAGAATTGTGAAAATTTGGTTACTTAATTCTTGCATGTTTAGGAGTGACTTTGAAATGGTCAAAAATCACTTCTTAGTGATTTTGCCATTCCAAACTTACTCTCAAACATGCTTGAGGAGTTTTGAAATGGTTAGGATTACTTTCATCATGTTCGAACCTACTCCAGAACATGCTTTAATCATTCATTCCAAATCAACTTAATGTTTGATTTTACAATTTTCATACCGTACAAATTGATTTTGAGTAATTAAAAACATGTTTCAGAGTTATTTTAAATGCGACAAAAGTGATTTTATCTACCTCAAAGTGTTCTTTTTGAAGTAGTTATAGAAAGAGTTAGATACTTAGAAATTCATTACAAACCAGTCCTAAGTTATGGAAAAAAGTTCCAAAATCTATCTATCAAGGCATACAGTTTTCAAGATGTACCATGGAGACGAATAAACAATTACTGACTTTATGTTGATATGCAATCTGTTGTGGATAAGTTCTATGTCTGGTTTCTTCGTCTTACCCTCCAATAGTTGAATGGTACAGTTAAAGGGAGAAGATGGAAGATTCTCTGAGTTGACACTTTTTACCAATCTTTGATTGCAGATTATCCGGTATTCATTTTATGGCATGAAGGAGGCGTTTGGTTTTGCACCTTCATTGCTCCTATGGCTAAGGTACTGGCTCCTTATGTAGTGGAGTGGAGTACAATTGGTCGGTGTTCTCTGCATCTGTGATATTAAAACTGTTCTCAATCATATCATGTAGGTATAGCACATTCCTCTTGCTGTATGTAACAGGCATCACTAGTGAAGTTGGTTTAGTCTATCTGGCTCTTCCTTACATGAAGGTAAGAAATCCAATGTCATGTTGTACACATGATTCACTCTTACGAATTCAATCCCAAACCGATCTTGGGCATGCTGCGTCGAAGTATACGTCGTTCCATTAG

mRNA sequence

AAAAGATTCAAACTCCATGAAGCCCTAGGATGGAAGCGATAATTTGTACCACTTGTCATAATTATATTAGAAAGATATCTTCTGGAGGTGGGCGATCCGATCAGACGGCAATCACTGAAGTAGTTCAGCTGCTCAACTAAATGCTATGTACTCATTTTCTCATAAGATTCTCCAGATCTTCAATCGTTCTCCATTTCTCCATCAATGGCGGGATTTTTGGCAACTATTCGGCGCCTGTACCTCTCCATCTACAATTGGACTCTCTTCGTCGGATGGTGTTCTACTTCTCAGTCAAGCAATTGAAGGAATCTGGTCACCAACAGGTATATAATGCCGTCGAACGTCCTCTTCAGTTTGCTCAAACTGCAGCTGTTTTAGAGATTCTCCATGGCCTCGTTGGGTTTGTGAGATCCCCTGTAACAGCAACGTTACCACAGATAGGTTCTAGATTGTATTTGACTTGGGGCATCTTATGGAGTTTCCCTGAGATTATCCGGTATTCATTTTATGGCATGAAGGAGGCGTTTGGTTTTGCACCTTCATTGCTCCTATGGCTAAGGTATAGCACATTCCTCTTGCTGTATGTAACAGGCATCACTAGTGAAGTTGGTTTAGTCTATCTGGCTCTTCCTTACATGAAGGTAAGAAATCCAATGTCATGTTGTACACATGATTCACTCTTACGAATTCAATCCCAAACCGATCTTGGGCATGCTGCGTCGAAGTATACGTCGTTCCATTAG

Coding sequence (CDS)

ATGCTATGTACTCATTTTCTCATAAGATTCTCCAGATCTTCAATCGTTCTCCATTTCTCCATCAATGGCGGGATTTTTGGCAACTATTCGGCGCCTGTACCTCTCCATCTACAATTGGACTCTCTTCGTCGGATGGTGTTCTACTTCTCAGTCAAGCAATTGAAGGAATCTGGTCACCAACAGGTATATAATGCCGTCGAACGTCCTCTTCAGTTTGCTCAAACTGCAGCTGTTTTAGAGATTCTCCATGGCCTCGTTGGGTTTGTGAGATCCCCTGTAACAGCAACGTTACCACAGATAGGTTCTAGATTGTATTTGACTTGGGGCATCTTATGGAGTTTCCCTGAGATTATCCGGTATTCATTTTATGGCATGAAGGAGGCGTTTGGTTTTGCACCTTCATTGCTCCTATGGCTAAGGTATAGCACATTCCTCTTGCTGTATGTAACAGGCATCACTAGTGAAGTTGGTTTAGTCTATCTGGCTCTTCCTTACATGAAGGTAAGAAATCCAATGTCATGTTGTACACATGATTCACTCTTACGAATTCAATCCCAAACCGATCTTGGGCATGCTGCGTCGAAGTATACGTCGTTCCATTAG

Protein sequence

MLCTHFLIRFSRSSIVLHFSINGGIFGNYSAPVPLHLQLDSLRRMVFYFSVKQLKESGHQQVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLYLTWGILWSFPEIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLLYVTGITSEVGLVYLALPYMKVRNPMSCCTHDSLLRIQSQTDLGHAASKYTSFH

Homology

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BLAST of Carg25856 vs. NCBI nr
Match: XP_022942934.1 (very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2A-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 322.8 bits (826), Expect = 8.3e-85
Identity = 167/185 (90.27%), Postives = 167/185 (90.27%), Query Frame = 0


Query: 1   MLCTHFLIRFSRSSIVLHFSINGGIFGNYSAPVPLHLQLDSLRRMVFYFSVKQLKESGHQ 60
           MLCTHFLIRFSRSSIVLHFSINGGIFGNYSAPVPLHLQLDSLRRMVFYFSVKQLKESGHQ
Sbjct: 1   MLCTHFLIRFSRSSIVLHFSINGGIFGNYSAPVPLHLQLDSLRRMVFYFSVKQLKESGHQ 60

Query: 61  QVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLYLTWGILWSFP----- 120
           QVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLYLTWGILWSFP     
Sbjct: 61  QVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLYLTWGILWSFPEVRNH 120

Query: 121 -------------EIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLLYVTGITSEVGLVYLA 168
                        EIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLLYVTGITSEVGLVYLA
Sbjct: 121 VLVSSLVISWSITEIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLLYVTGITSEVGLVYLA 180

[Close]

BLAST of Carg25856 vs. NCBI nr
Match: XP_022942201.1 (very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2A-like isoform X1 [Cucurbita moschata] >XP_022942202.1 very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2A-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 320.5 bits (820), Expect = 4.1e-84
Identity = 166/185 (89.73%), Postives = 166/185 (89.73%), Query Frame = 0


Query: 1   MLCTHFLIRFSRSSIVLHFSINGGIFGNYSAPVPLHLQLDSLRRMVFYFSVKQLKESGHQ 60
           MLCTHFLIRFSRSSIVLHFSINGGIFGNYSAPVPLHLQLDSLRRMVFYFSVKQLKESGHQ
Sbjct: 1   MLCTHFLIRFSRSSIVLHFSINGGIFGNYSAPVPLHLQLDSLRRMVFYFSVKQLKESGHQ 60

Query: 61  QVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLYLTWGILWSFP----- 120
           QVYNAVERPLQFAQTAAVLEILHGLVG VRSPVTATLPQIGSRLYLTWGILWSFP     
Sbjct: 61  QVYNAVERPLQFAQTAAVLEILHGLVGLVRSPVTATLPQIGSRLYLTWGILWSFPEVRNH 120

Query: 121 -------------EIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLLYVTGITSEVGLVYLA 168
                        EIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLLYVTGITSEVGLVYLA
Sbjct: 121 VLVSSLVISWSITEIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLLYVTGITSEVGLVYLA 180

[Close]

BLAST of Carg25856 vs. NCBI nr
Match: XP_022975511.1 (very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2A isoform X1 [Cucurbita maxima] >XP_022975530.1 very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2A isoform X1 [Cucurbita maxima])

HSP 1 Score: 313.9 bits (803), Expect = 3.9e-82
Identity = 162/185 (87.57%), Postives = 165/185 (89.19%), Query Frame = 0


Query: 1   MLCTHFLIRFSRSSIVLHFSINGGIFGNYSAPVPLHLQLDSLRRMVFYFSVKQLKESGHQ 60
           MLCTHFL+RFS+SSIVLHFSING IFGNYSAPVPLHLQLDSLRRMVFYFSVKQLKESGHQ
Sbjct: 1   MLCTHFLLRFSKSSIVLHFSINGRIFGNYSAPVPLHLQLDSLRRMVFYFSVKQLKESGHQ 60

Query: 61  QVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLYLTWGILWSFP----- 120
           QVYNAVERPLQFAQTAAVLEILHGLVG VRSPVTATLPQIGSRLYLTWGILWSFP     
Sbjct: 61  QVYNAVERPLQFAQTAAVLEILHGLVGLVRSPVTATLPQIGSRLYLTWGILWSFPEVRNH 120

Query: 121 -------------EIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLLYVTGITSEVGLVYLA 168
                        EIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLLYVTGITSEVGLVYLA
Sbjct: 121 VLVSSLVISWSITEIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLLYVTGITSEVGLVYLA 180

[Close]

BLAST of Carg25856 vs. NCBI nr
Match: XP_022942935.1 (very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2A-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 233.4 bits (594), Expect = 6.6e-58
Identity = 122/140 (87.14%), Postives = 122/140 (87.14%), Query Frame = 0


Query: 46  VFYFSVKQLKESGHQQVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLY 105
           VFYFSVKQLKESGHQQVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLY
Sbjct: 27  VFYFSVKQLKESGHQQVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLY 86

Query: 106 LTWGILWSFP------------------EIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLL 165
           LTWGILWSFP                  EIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLL
Sbjct: 87  LTWGILWSFPEVRNHVLVSSLVISWSITEIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLL 146

Query: 166 YVTGITSEVGLVYLALPYMK 168
           YVTGITSEVGLVYLALPYMK
Sbjct: 147 YVTGITSEVGLVYLALPYMK 166

[Close]

BLAST of Carg25856 vs. NCBI nr
Match: XP_022942204.1 (very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2A-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 231.1 bits (588), Expect = 3.3e-57
Identity = 121/140 (86.43%), Postives = 121/140 (86.43%), Query Frame = 0


Query: 46  VFYFSVKQLKESGHQQVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLY 105
           VFYFSVKQLKESGHQQVYNAVERPLQFAQTAAVLEILHGLVG VRSPVTATLPQIGSRLY
Sbjct: 27  VFYFSVKQLKESGHQQVYNAVERPLQFAQTAAVLEILHGLVGLVRSPVTATLPQIGSRLY 86

Query: 106 LTWGILWSFP------------------EIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLL 165
           LTWGILWSFP                  EIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLL
Sbjct: 87  LTWGILWSFPEVRNHVLVSSLVISWSITEIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLL 146

Query: 166 YVTGITSEVGLVYLALPYMK 168
           YVTGITSEVGLVYLALPYMK
Sbjct: 147 YVTGITSEVGLVYLALPYMK 166

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BLAST of Carg25856 vs. TAIR10
Match: AT5G10480.3 (Protein-tyrosine phosphatase-like, PTPLA)

HSP 1 Score: 179.5 bits (454), Expect = 2.1e-45
Identity = 94/149 (63.09%), Postives = 110/149 (73.83%), Query Frame = 0


Query: 46  VFYFSVKQLKESGHQQVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLY 105
           V Y ++  LKE+G++ VY+A+E+PLQ AQTAAVLEILHGLVG VRSPV+ATLPQIGSRL+
Sbjct: 27  VLYLAITTLKETGYENVYDAIEKPLQLAQTAAVLEILHGLVGLVRSPVSATLPQIGSRLF 86

Query: 106 LTWGILWSFP---------------------------EIIRYSFYGMKEAFGFAPSLLLW 165
           LTWGIL+SFP                           EIIRYSF+G KEA GFAPS  LW
Sbjct: 87  LTWGILYSFPEVRSHFLVTSLVISWSITEFPITTWIVEIIRYSFFGFKEALGFAPSWHLW 146

Query: 166 LRYSTFLLLYVTGITSEVGLVYLALPYMK 168
           LRYS+FLLLY TGITSEVGL+YLALP++K
Sbjct: 147 LRYSSFLLLYPTGITSEVGLIYLALPHIK 175

[Close]

BLAST of Carg25856 vs. TAIR10
Match: AT5G59770.1 (Protein-tyrosine phosphatase-like, PTPLA)

HSP 1 Score: 63.2 bits (152), Expect = 2.2e-10
Identity = 39/113 (34.51%), Postives = 56/113 (49.56%), Query Frame = 0


Query: 76  AAVLEILHGLVGFVRSPVTATLPQIGSRLYLTWGIL------------------WSFPEI 135
           AA+LE+LHG +G V S   + L Q   R +    I+                  W   E+
Sbjct: 105 AAILEVLHGAIGIVPSGFLSPLMQWSGRTHFILAIVGQIKEVQDSPWLSITLVAWCIGEM 164

Query: 136 IRYSFYGMKEAFGFAPSLLLWLRYSTFLLLYVTGITSEVGLVYLALPYMKVRN 171
           IRY  Y      G  P  L +LRY+ F+++Y TG+  E+ ++Y ALPY+K RN
Sbjct: 165 IRYPHYAF-TCLGRCPYWLTYLRYTGFIVIYPTGLVGELLIMYKALPYVKERN 216

[Close]

BLAST of Carg25856 vs. Swiss-Prot
Match: sp|Q7XSZ4|HACDA_ORYSJ (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2A OS=Oryza sativa subsp. japonica OX=39947 GN=PAS2A PE=2 SV=2)

HSP 1 Score: 196.8 bits (499), Expect = 2.3e-49
Identity = 101/140 (72.14%), Postives = 112/140 (80.00%), Query Frame = 0


Query: 46  VFYFSVKQLKESGHQQVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLY 105
           V Y++V  L ESGH+ VY AVERPLQFAQTAA LEILHGLVG VRSPV+ATLPQIGSRL+
Sbjct: 27  VLYYAVTTLLESGHEAVYAAVERPLQFAQTAAFLEILHGLVGLVRSPVSATLPQIGSRLF 86

Query: 106 LTWGILWSFP------------------EIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLL 165
           LTWGILWSFP                  EIIRYSF+GMKEAFGFAPS LLWLRYSTF++L
Sbjct: 87  LTWGILWSFPETHSHILVTSLVISWSITEIIRYSFFGMKEAFGFAPSWLLWLRYSTFMVL 146

Query: 166 YVTGITSEVGLVYLALPYMK 168
           Y TGI+SEVGL+Y+ALPYMK
Sbjct: 147 YPTGISSEVGLIYIALPYMK 166

[Close]

BLAST of Carg25856 vs. Swiss-Prot
Match: sp|Q5ZEJ0|HACDB_ORYSJ (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2B OS=Oryza sativa subsp. japonica OX=39947 GN=PAS2B PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 6.6e-49
Identity = 100/140 (71.43%), Postives = 111/140 (79.29%), Query Frame = 0


Query: 46  VFYFSVKQLKESGHQQVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLY 105
           V Y++V  L ESGH+ VY AVERPLQFAQTAA LEILHGLVG VRSPV+ATLPQIGSRL+
Sbjct: 27  VLYYAVTTLLESGHEAVYAAVERPLQFAQTAAFLEILHGLVGLVRSPVSATLPQIGSRLF 86

Query: 106 LTWGILWSFP------------------EIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLL 165
           LTWGILWSFP                  EIIRYSF+GMKE FGFAPS LLWLRYSTF++L
Sbjct: 87  LTWGILWSFPETHSHILVTSLVISWSITEIIRYSFFGMKETFGFAPSWLLWLRYSTFMVL 146

Query: 166 YVTGITSEVGLVYLALPYMK 168
           Y TGI+SEVGL+Y+ALPYMK
Sbjct: 147 YPTGISSEVGLIYIALPYMK 166

[Close]

BLAST of Carg25856 vs. Swiss-Prot
Match: sp|Q8VZB2|HACD_ARATH (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2 OS=Arabidopsis thaliana OX=3702 GN=PAS2 PE=1 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 3.4e-45
Identity = 94/140 (67.14%), Postives = 110/140 (78.57%), Query Frame = 0


Query: 46  VFYFSVKQLKESGHQQVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLY 105
           V Y ++  LKE+G++ VY+A+E+PLQ AQTAAVLEILHGLVG VRSPV+ATLPQIGSRL+
Sbjct: 27  VLYLAITTLKETGYENVYDAIEKPLQLAQTAAVLEILHGLVGLVRSPVSATLPQIGSRLF 86

Query: 106 LTWGILWSFP------------------EIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLL 165
           LTWGIL+SFP                  EIIRYSF+G KEA GFAPS  LWLRYS+FLLL
Sbjct: 87  LTWGILYSFPEVRSHFLVTSLVISWSITEIIRYSFFGFKEALGFAPSWHLWLRYSSFLLL 146

Query: 166 YVTGITSEVGLVYLALPYMK 168
           Y TGITSEVGL+YLALP++K
Sbjct: 147 YPTGITSEVGLIYLALPHIK 166

[Close]

BLAST of Carg25856 vs. Swiss-Prot
Match: sp|Q9D3B1|HACD2_MOUSE (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 OS=Mus musculus OX=10090 GN=Hacd2 PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 5.1e-17
Identity = 49/132 (37.12%), Postives = 72/132 (54.55%), Query Frame = 0


Query: 54  LKESGHQQVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLYLTWG---- 113
           L +  +  +Y ++ERPL+F QT A+LEILH  +G V S V  T  Q+ SR++L W     
Sbjct: 65  LAKGSYHSLYYSIERPLKFFQTGALLEILHCAIGIVPSSVVLTSFQVMSRVFLIWAVTHS 124

Query: 114 --------------ILWSFPEIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLLYVTGITSE 168
                         I W+  EIIRYSFY         P ++ W RY+ F++LY  G+T E
Sbjct: 125 VKEVQSEDSVLLFVIAWTITEIIRYSFYTF-SLLNHLPYIIKWARYTLFIVLYPMGVTGE 184

[Close]

BLAST of Carg25856 vs. Swiss-Prot
Match: sp|Q2KIP8|HACD2_BOVIN (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 OS=Bos taurus OX=9913 GN=HACD2 PE=2 SV=2)

HSP 1 Score: 87.8 bits (216), Expect = 1.5e-16
Identity = 48/132 (36.36%), Postives = 72/132 (54.55%), Query Frame = 0


Query: 54  LKESGHQQVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLYLTWG---- 113
           L +  +  +Y ++E+PL+F QT A+LEILH  +G V S V  T  Q+ SR++L W     
Sbjct: 65  LAKGSYHSLYYSIEKPLKFFQTGALLEILHCAIGIVPSSVVLTSIQVMSRVFLIWAVTHS 124

Query: 114 --------------ILWSFPEIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLLYVTGITSE 168
                         I W+  EIIRYSFY         P L+ W RY+ F++LY  G++ E
Sbjct: 125 VKEVQSEDSVLLFVIAWTITEIIRYSFYTF-SLLNHLPYLIKWARYTLFIVLYPMGVSGE 184

[Close]

BLAST of Carg25856 vs. TrEMBL
Match: tr|A0A1S3BT98|A0A1S3BT98_CUCME (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Cucumis melo OX=3656 GN=LOC103493277 PE=3 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.3e-54
Identity = 113/140 (80.71%), Postives = 119/140 (85.00%), Query Frame = 0


Query: 46  VFYFSVKQLKESGHQQVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLY 105
           VFYF +KQLKESGHQQVYNAVERPLQF+QTAAVLEILHGLVG VRSPVTATLPQIGSRLY
Sbjct: 27  VFYFCIKQLKESGHQQVYNAVERPLQFSQTAAVLEILHGLVGLVRSPVTATLPQIGSRLY 86

Query: 106 LTWGILWSFP------------------EIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLL 165
           +TWGILWSFP                  EIIRY+FYGMKE FGFAPSLLLWLRYSTFLLL
Sbjct: 87  VTWGILWSFPEVRNHILVTSLVVSWSITEIIRYAFYGMKETFGFAPSLLLWLRYSTFLLL 146

Query: 166 YVTGITSEVGLVYLALPYMK 168
           YVTGI+SEVGL+YLALPYMK
Sbjct: 147 YVTGISSEVGLIYLALPYMK 166

[Close]

BLAST of Carg25856 vs. TrEMBL
Match: tr|A0A0A0L3F8|A0A0A0L3F8_CUCSA (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Cucumis sativus OX=3659 GN=Csa_3G114430 PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 3.3e-53
Identity = 109/140 (77.86%), Postives = 118/140 (84.29%), Query Frame = 0


Query: 46  VFYFSVKQLKESGHQQVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLY 105
           VFYF +KQLKESGHQ VYNAVERPLQF+QTAAVLEILHGLVG VRSPV+ATLPQIGSRLY
Sbjct: 27  VFYFCIKQLKESGHQHVYNAVERPLQFSQTAAVLEILHGLVGLVRSPVSATLPQIGSRLY 86

Query: 106 LTWGILWSFP------------------EIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLL 165
           +TWGILWSFP                  EIIRY+FYGMKE FGFAPSLL+WLRYSTFLLL
Sbjct: 87  VTWGILWSFPEVRNHILVTSLVISWSITEIIRYAFYGMKETFGFAPSLLIWLRYSTFLLL 146

Query: 166 YVTGITSEVGLVYLALPYMK 168
           YVTGI+SE+GL+YLALPYMK
Sbjct: 147 YVTGISSEIGLIYLALPYMK 166

[Close]

BLAST of Carg25856 vs. TrEMBL
Match: tr|A0A061DI73|A0A061DI73_THECC (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase (Fragment) OS=Theobroma cacao OX=3641 GN=TCM_000991 PE=3 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.8e-51
Identity = 107/164 (65.24%), Postives = 125/164 (76.22%), Query Frame = 0


Query: 22  NGGIFGNYSAPVPLHLQLDSLRRMVFYFSVKQLKESGHQQVYNAVERPLQFAQTAAVLEI 81
           +G  F    AP+P HLQLD L R+V + ++K LKESGH+ VYNAVE+PL  AQ+AA LEI
Sbjct: 57  HGHPFRCSQAPLPFHLQLDRLHRLVLFLALKSLKESGHEHVYNAVEKPLLLAQSAAALEI 116

Query: 82  LHGLVGFVRSPVTATLPQIGSRLYLTWGILWSFP------------------EIIRYSFY 141
           LHGL+G VRSPV++TLPQ+GSRLYLTWGILWSFP                  EIIRYSF+
Sbjct: 117 LHGLIGLVRSPVSSTLPQVGSRLYLTWGILWSFPEIRTHILVSSLVISWSITEIIRYSFF 176

Query: 142 GMKEAFGFAPSLLLWLRYSTFLLLYVTGITSEVGLVYLALPYMK 168
           GMKEAFGFAPS  LWLRYS+FL+LY TGI+SEVGLVY ALPY+K
Sbjct: 177 GMKEAFGFAPSWHLWLRYSSFLVLYPTGISSEVGLVYFALPYIK 220

[Close]

BLAST of Carg25856 vs. TrEMBL
Match: tr|A0A0D3B0H9|A0A0D3B0H9_BRAOL (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Brassica oleracea var. oleracea OX=109376 PE=3 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 1.3e-49
Identity = 110/168 (65.48%), Postives = 128/168 (76.19%), Query Frame = 0


Query: 21  INGGIFGNYSAPVPLHLQLDSLRRM---VFYFSVKQLKESGHQQVYNAVERPLQFAQTAA 80
           ++GG+F    + VP  LQLD L RM   + YF+VK LKESGH+ VY AVE+PLQ AQTAA
Sbjct: 31  VHGGVFLLRPSRVPHSLQLDRLCRMASQILYFAVKTLKESGHENVYEAVEKPLQLAQTAA 90

Query: 81  VLEILHGLVGFVRSPVTATLPQIGSRLYLTWGILWSFP------------------EIIR 140
           VLEILHGLVG VRSPV+ATLPQIGSRL+LTWGIL+SFP                  EIIR
Sbjct: 91  VLEILHGLVGLVRSPVSATLPQIGSRLFLTWGILYSFPEVQSHFLVASLVISWSITEIIR 150

Query: 141 YSFYGMKEAFGFAPSLLLWLRYSTFLLLYVTGITSEVGLVYLALPYMK 168
           YSF+G+KEA GFAPS  LWLRYS+FL+LY TGITSEVGL+YLALP++K
Sbjct: 151 YSFFGLKEALGFAPSWHLWLRYSSFLVLYPTGITSEVGLIYLALPHIK 198

[Close]

BLAST of Carg25856 vs. TrEMBL
Match: tr|A0A2I4G7B5|A0A2I4G7B5_9ROSI (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Juglans regia OX=51240 GN=LOC109005346 PE=3 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.1e-48
Identity = 102/140 (72.86%), Postives = 114/140 (81.43%), Query Frame = 0


Query: 46  VFYFSVKQLKESGHQQVYNAVERPLQFAQTAAVLEILHGLVGFVRSPVTATLPQIGSRLY 105
           VFY +VK ++ESGH+QVYNAVERPLQFAQTAA+LEI+HGLVG VRSP++ATLPQI SRLY
Sbjct: 27  VFYLAVKTVRESGHEQVYNAVERPLQFAQTAALLEIIHGLVGLVRSPISATLPQISSRLY 86

Query: 106 LTWGILWSFP------------------EIIRYSFYGMKEAFGFAPSLLLWLRYSTFLLL 165
           LTWGILWSFP                  EIIRYSF+GMKE  GFAPS LLWLRYSTFLLL
Sbjct: 87  LTWGILWSFPQTQGHVLVTSLVISWSITEIIRYSFFGMKELLGFAPSWLLWLRYSTFLLL 146

Query: 166 YVTGITSEVGLVYLALPYMK 168
           Y TGITSEVGL+Y+ALPY+K
Sbjct: 147 YPTGITSEVGLIYVALPYIK 166

The following BLAST results are available for this feature:

BLAST of Carg25856 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022942934.1	8.3e-85	90.27	very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2A-like isoform X1...	[more]
XP_022942201.1	4.1e-84	89.73	very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2A-like isoform X1...	[more]
XP_022975511.1	3.9e-82	87.57	very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2A isoform X1 [Cuc...	[more]
XP_022942935.1	6.6e-58	87.14	very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2A-like isoform X2...	[more]
XP_022942204.1	3.3e-57	86.43	very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2A-like isoform X2...	[more]

BLAST of Carg25856 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G10480.3	2.1e-45	63.09	Protein-tyrosine phosphatase-like, PTPLA	[more]
AT5G59770.1	2.2e-10	34.51	Protein-tyrosine phosphatase-like, PTPLA	[more]

BLAST of Carg25856 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q7XSZ4\|HACDA_ORYSJ	2.3e-49	72.14	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2A OS=Oryza sativa...	[more]
sp\|Q5ZEJ0\|HACDB_ORYSJ	6.6e-49	71.43	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2B OS=Oryza sativa...	[more]
sp\|Q8VZB2\|HACD_ARATH	3.4e-45	67.14	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2 OS=Arabidopsis t...	[more]
sp\|Q9D3B1\|HACD2_MOUSE	5.1e-17	37.12	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 OS=Mus musculus OX=10090 GN...	[more]
sp\|Q2KIP8\|HACD2_BOVIN	1.5e-16	36.36	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 OS=Bos taurus OX=9913 GN=HA...	[more]

BLAST of Carg25856 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BT98\|A0A1S3BT98_CUCME	1.3e-54	80.71	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Cucumis melo OX=3656 GN=LO...	[more]
tr\|A0A0A0L3F8\|A0A0A0L3F8_CUCSA	3.3e-53	77.86	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Cucumis sativus OX=3659 GN...	[more]
tr\|A0A061DI73\|A0A061DI73_THECC	1.8e-51	65.24	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase (Fragment) OS=Theobroma cacao...	[more]
tr\|A0A0D3B0H9\|A0A0D3B0H9_BRAOL	1.3e-49	65.48	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Brassica oleracea var. ole...	[more]
tr\|A0A2I4G7B5\|A0A2I4G7B5_9ROSI	1.1e-48	72.86	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Juglans regia OX=51240 GN=...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR007482	Tyr_Pase-like_PTPLA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25856-RA	Carg25856-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007482	Protein-tyrosine phosphatase-like, PTPLA	PFAM	PF04387	PTPLA	coord: 74..116 e-value: 1.5E-12 score: 47.5 coord: 110..173 e-value: 2.3E-18 score: 66.4
IPR007482	Protein-tyrosine phosphatase-like, PTPLA	PANTHER	PTHR11035	PTPLA DOMAIN PROTEIN	coord: 45..169
None	No IPR available	PANTHER	PTHR11035:SF3	VERY-LONG-CHAIN 3R -3-HYDROXYACYL-COA DEHYDRATASE HPO-8	coord: 45..169

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg25856	Cla004547	Watermelon (97103) v1	carwmB0011
Carg25856	Csa3G114430	Cucumber (Chinese Long) v2	carcuB0009
Carg25856	MELO3C016419	Melon (DHL92) v3.5.1	carmeB0007
Carg25856	ClCG10G007720	Watermelon (Charleston Gray)	carwcgB0009
Carg25856	CSPI03G06730	Wild cucumber (PI 183967)	carcpiB0009
Carg25856	Cucsa.260310	Cucumber (Gy14) v1	carcgyB0008
Carg25856	CmaCh04G008660	Cucurbita maxima (Rimu)	carcmaB0015
Carg25856	CmoCh04G009290	Cucurbita moschata (Rifu)	carcmoB0015
Carg25856	Lsi05G007780	Bottle gourd (USVL1VR-Ls)	carlsiB008
Carg25856	Cp4.1LG01g08810	Cucurbita pepo (Zucchini)	carcpeB0013
Carg25856	CsGy3G006860	Cucumber (Gy14) v2	carcgybB0008
Carg25856	MELO3C016419.2	Melon (DHL92) v3.6.1	carmedB0008
Carg25856	CsaV3_3G006860	Cucumber (Chinese Long) v3	carcucB0009
Carg25856	Cla97C10G191380	Watermelon (97103) v2	carwmbB0008
Carg25856	Bhi11G000790	Wax gourd	carwgoB0010

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None