Carg24984 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg24984
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Heat stress transcription factor
Location	Cucurbita_argyrosperma_scaffold_187 : 67322 .. 68897 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGCCGGAGTGAGCAATAATAACGGTGATCAGAAGGGGTTGCGGTTGCCGTCGCCGCCGCGGACGGTGGAGAGTATGATGAAAAAGTCAACGCCGCCGCCGTTTCTGGTGAAGACTTATACGGTGGTGGAGGATCCGGCGACCGACAAGGTGATATCGTGGAACGGCGAGGGGACGGCGTTTGTGGTTTGGCAGACGGCGGAGTTCGCAAAGGATGTTTTGCCGAAGCTCTTTAAACACTGCAATTTCTCAAGCTTTGTTCGCCAGCTTAATACTTACGTACGTATTCTTTTTATTTTATTTTCTTTATCTTTTTTATTTTAAACCTATTAAATTTTTTTTCCTTTAATTTTCGGGTTGAATTACAAACACGTGAGCGGAAATTTGAATAGACAAAACCCGAACGACGACAAAGGAAGAACGATCATCTTAGATTAAGAAAAATTGTAGAAAATATTTTTCTCGAGAAGTTTAATTTCTGATCCTTTTAATCGTGTTTAATGATTAATGAGTTAAAACATTAAAAGCTTCTAATAAATTTATAAATTTTAAATAAATTTTTGTTCGAGCAATTTTTAAGGTTGTCATAGAACAGATATCCTAACTACTTAACTATGTTATTATTAAAAATCCCGAGTGGTTATAATTTTATAAATTTATTTAAATTTTGATACTTTAAAAAATAATAAAAATTGAATAAATAATATTTTCTTTTAAATATTAAGAAAGAAAACAAAAATAATGCAAAAACTCAAGTCAACAAAAAAAAAATTTAATTTTCCATTTTTATTAAATACTTTACAAACCTAAACCCATTTTCCAAAATTTTATTTTATTTTATTTTTATTTTTTACCAACCCATTATTATTTTTTAAATAAAAAAGTAGGGTCCTTACCTGCCACGTGTCATTTAATTGCAAATGATTGACTTGGTCAAAGCCAATATGAATTAATTATTCTTATATGTAACTTTAATTATATTTATTTATTTATTGTAAAATTACCATCAATAATATGAACTTAACCCATCTTAGAAGAAAAGATTAAGGACAAAATCGTAAATTTAGGGTTTAAGGTTTATAAGTCGATGATAAGCTGATCGATCCTTCTCTCAAAGTTTAAATTTTTATTGAAGGGGTTTCGAAAGGTGACGACAACAAGGTGGGAGTTTTGCAACGACAAATTTCGAAAGGGTGAAAAGGAAAAGCTTTGTGAAATACGAAGACGAAAGGCATGGACGAACAAGCAACAACACGAGGTACAACACGAGGTCGATGAAGATCAGAGATCTTCGTCTTCGAACTCATCGTCGTCACATTACAATACGCTAATGGATGAGAACAAGAGGTTGAAGAAGGAAAATGGGGCCTTGAGCTTAGAGCTTGCAAGCATGAAAGGGAAGTGCAGAGAGCTTCTTGATTTGGTGGCTAAGTATGGGAATGGTTCAAGGAAAGAAGATGAAGATGAGAGGCCAAAGCTGTTTGGTGTGAGGTTGGAAGTTGAAGGAGAGAGGAGGAGGAAGACAAAAAGGGCTGAAGAGACAGCTTTTTTGCCTTCTCAATCATGCAAATAG

mRNA sequence

ATGGAGGCCGGAGTGAGCAATAATAACGGTGATCAGAAGGGGTTGCGGTTGCCGTCGCCGCCGCGGACGGTGGAGAGTATGATGAAAAAGTCAACGCCGCCGCCGTTTCTGGTGAAGACTTATACGGTGGTGGAGGATCCGGCGACCGACAAGGTGATATCGTGGAACGGCGAGGGGACGGCGTTTGTGGTTTGGCAGACGGCGGAGTTCGCAAAGGATGTTTTGCCGAAGCTCTTTAAACACTGCAATTTCTCAAGCTTTGTTCGCCAGCTTAATACTTACGGGTTTCGAAAGGTGACGACAACAAGGTGGGAGTTTTGCAACGACAAATTTCGAAAGGGTGAAAAGGAAAAGCTTTGTGAAATACGAAGACGAAAGGCATGGACGAACAAGCAACAACACGAGGTACAACACGAGGTCGATGAAGATCAGAGATCTTCGTCTTCGAACTCATCGTCGTCACATTACAATACGCTAATGGATGAGAACAAGAGGTTGAAGAAGGAAAATGGGGCCTTGAGCTTAGAGCTTGCAAGCATGAAAGGGAAGTGCAGAGAGCTTCTTGATTTGGTGGCTAAGTATGGGAATGGTTCAAGGAAAGAAGATGAAGATGAGAGGCCAAAGCTGTTTGGTGTGAGGTTGGAAGTTGAAGGAGAGAGGAGGAGGAAGACAAAAAGGGCTGAAGAGACAGCTTTTTTGCCTTCTCAATCATGCAAATAG

Coding sequence (CDS)

ATGGAGGCCGGAGTGAGCAATAATAACGGTGATCAGAAGGGGTTGCGGTTGCCGTCGCCGCCGCGGACGGTGGAGAGTATGATGAAAAAGTCAACGCCGCCGCCGTTTCTGGTGAAGACTTATACGGTGGTGGAGGATCCGGCGACCGACAAGGTGATATCGTGGAACGGCGAGGGGACGGCGTTTGTGGTTTGGCAGACGGCGGAGTTCGCAAAGGATGTTTTGCCGAAGCTCTTTAAACACTGCAATTTCTCAAGCTTTGTTCGCCAGCTTAATACTTACGGGTTTCGAAAGGTGACGACAACAAGGTGGGAGTTTTGCAACGACAAATTTCGAAAGGGTGAAAAGGAAAAGCTTTGTGAAATACGAAGACGAAAGGCATGGACGAACAAGCAACAACACGAGGTACAACACGAGGTCGATGAAGATCAGAGATCTTCGTCTTCGAACTCATCGTCGTCACATTACAATACGCTAATGGATGAGAACAAGAGGTTGAAGAAGGAAAATGGGGCCTTGAGCTTAGAGCTTGCAAGCATGAAAGGGAAGTGCAGAGAGCTTCTTGATTTGGTGGCTAAGTATGGGAATGGTTCAAGGAAAGAAGATGAAGATGAGAGGCCAAAGCTGTTTGGTGTGAGGTTGGAAGTTGAAGGAGAGAGGAGGAGGAAGACAAAAAGGGCTGAAGAGACAGCTTTTTTGCCTTCTCAATCATGCAAATAG

Protein sequence

MEAGVSNNNGDQKGLRLPSPPRTVESMMKKSTPPPFLVKTYTVVEDPATDKVISWNGEGTAFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLCEIRRRKAWTNKQQHEVQHEVDEDQRSSSSNSSSSHYNTLMDENKRLKKENGALSLELASMKGKCRELLDLVAKYGNGSRKEDEDERPKLFGVRLEVEGERRRKTKRAEETAFLPSQSCK

Homology

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BLAST of Carg24984 vs. NCBI nr
Match: XP_022928625.1 (heat stress transcription factor B-3 [Cucurbita moschata])

HSP 1 Score: 420.6 bits (1080), Expect = 3.5e-114
Identity = 234/239 (97.91%), Postives = 234/239 (97.91%), Query Frame = 0


Query: 1   MEAGVSNNNGDQKGLRLPSPPRTVESMMKKSTPPPFLVKTYTVVEDPATDKVISWNGEGT 60
           MEAGVSNNNGDQKGLRLPSPPRTVESMMKKSTPPPFLVKTYTVVEDPATDKVISWN EGT
Sbjct: 1   MEAGVSNNNGDQKGLRLPSPPRTVESMMKKSTPPPFLVKTYTVVEDPATDKVISWNSEGT 60

Query: 61  AFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLC 120
           AFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLC
Sbjct: 61  AFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLC 120

Query: 121 EIRRRKAWTNKQQHEVQXXXXXXXXXXXXXXXXXXXXXLMDENKRLKKENGALSLELASM 180
           EIRRRKAWTNKQQHEVQXXXXXXXXXXXXXXXXXXXXX   ENKRLKKENGALS ELASM
Sbjct: 121 EIRRRKAWTNKQQHEVQXXXXXXXXXXXXXXXXXXXXXXXXENKRLKKENGALSSELASM 180

Query: 181 KGKCRELLDLVAKYGNGSRKEDEDERPKLFGVRLEVEGERRRKTKRAEETAFLPSQSCK 240
           KGKCRELLDLVAKYGNGSRKEDEDERPKLFGVRLEVEGERRRKTKRAEETAFLPSQSCK
Sbjct: 181 KGKCRELLDLVAKYGNGSRKEDEDERPKLFGVRLEVEGERRRKTKRAEETAFLPSQSCK 239

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BLAST of Carg24984 vs. NCBI nr
Match: XP_023544792.1 (heat stress transcription factor B-3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 418.3 bits (1074), Expect = 1.7e-113
Identity = 235/241 (97.51%), Postives = 236/241 (97.93%), Query Frame = 0


Query: 1   MEAGVSNNNGDQKGLR--LPSPPRTVESMMKKSTPPPFLVKTYTVVEDPATDKVISWNGE 60
           MEAGVS+NNGDQKGLR  LPSPPRTVESMMKKSTPPPFLVKTYTVVEDPATDKVISWNGE
Sbjct: 1   MEAGVSDNNGDQKGLRLPLPSPPRTVESMMKKSTPPPFLVKTYTVVEDPATDKVISWNGE 60

Query: 61  GTAFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEK 120
           GTAFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEK
Sbjct: 61  GTAFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEK 120

Query: 121 LCEIRRRKAWTNKQQHEVQXXXXXXXXXXXXXXXXXXXXXLMDENKRLKKENGALSLELA 180
           LCEIRRRKAWTNKQQHEV XXXXXXXXXXXXXXXXXXXXXLMDENKRLKKENG LS ELA
Sbjct: 121 LCEIRRRKAWTNKQQHEVXXXXXXXXXXXXXXXXXXXXXXLMDENKRLKKENGDLSSELA 180

Query: 181 SMKGKCRELLDLVAKYGNGSRKEDEDERPKLFGVRLEVEGERRRKTKRAEETAFLPSQSC 240
           SMKGKCRELLDLVAKYGNGSRKEDEDERPKLFGVRLEVEGERRRKTKRAEETAFLPSQSC
Sbjct: 181 SMKGKCRELLDLVAKYGNGSRKEDEDERPKLFGVRLEVEGERRRKTKRAEETAFLPSQSC 240

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BLAST of Carg24984 vs. NCBI nr
Match: XP_022967894.1 (heat stress transcription factor B-3 [Cucurbita maxima])

HSP 1 Score: 403.3 bits (1035), Expect = 5.8e-109
Identity = 226/239 (94.56%), Postives = 228/239 (95.40%), Query Frame = 0


Query: 1   MEAGVSNNNGDQKGLRLPSPPRTVESMMKKSTPPPFLVKTYTVVEDPATDKVISWNGEGT 60
           MEAGVSNNNGDQKGLRLPSPPRTVESM+KKSTPPPFLVKTYTVVEDPATDKVISWNGEGT
Sbjct: 1   MEAGVSNNNGDQKGLRLPSPPRTVESMIKKSTPPPFLVKTYTVVEDPATDKVISWNGEGT 60

Query: 61  AFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLC 120
           AFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLC
Sbjct: 61  AFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLC 120

Query: 121 EIRRRKAWTNKQQHEVQXXXXXXXXXXXXXXXXXXXXXLMDENKRLKKENGALSLELASM 180
           EIRRRKAWTNKQQHEVQXXXXXXXXXXXXXXXXXXXXX       LKKENGALS ELASM
Sbjct: 121 EIRRRKAWTNKQQHEVQXXXXXXXXXXXXXXXXXXXXXXXXXXXXLKKENGALSSELASM 180

Query: 181 KGKCRELLDLVAKYGNGSRKEDEDERPKLFGVRLEVEGERRRKTKRAEETAFLPSQSCK 240
           K KCRELLDLVAKYGNGSRKE+EDERPKLFGVRLEVEGERRRKTKRAEET  LPSQSCK
Sbjct: 181 KEKCRELLDLVAKYGNGSRKEEEDERPKLFGVRLEVEGERRRKTKRAEETTLLPSQSCK 239

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BLAST of Carg24984 vs. NCBI nr
Match: XP_004149402.1 (PREDICTED: heat stress transcription factor B-3 [Cucumis sativus])

HSP 1 Score: 285.8 bits (730), Expect = 1.3e-73
Identity = 181/247 (73.28%), Postives = 190/247 (76.92%), Query Frame = 0


Query: 1   MEAGVSNNNGDQKGLRLPS--PPRTVES--MMKKSTPPPFLVKTYTVVEDPATDKVISWN 60
           ME GVSN              PPRT E+   M+KSTP PFL+KTY +VEDPATD VISWN
Sbjct: 1   MEDGVSNXXXXXXXXXXLGSWPPRTAENEIRMRKSTPAPFLMKTYRMVEDPATDDVISWN 60

Query: 61  GEGTAFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEK 120
            +GTAFVVWQTAEFAKDVLPKLFKH NFSSFVRQLNTYGFRKV TTRWEFCN+KF+KGEK
Sbjct: 61  SDGTAFVVWQTAEFAKDVLPKLFKHSNFSSFVRQLNTYGFRKVRTTRWEFCNEKFQKGEK 120

Query: 121 EKLCEIRRRKAWTNKQQHEVQXXXXXXXXXXXXXXXXXXXXXLMDENKRLKKENGALSLE 180
           EKLCEIRRRK W N       XXXXXXXXXXXXXXXXXX   LMDENKRLKKENGALS E
Sbjct: 121 EKLCEIRRRKKWRN----XXXXXXXXXXXXXXXXXXXXXYITLMDENKRLKKENGALSSE 180

Query: 181 LASMKGKCRELLDLVAKYGNGSRKEDEDERPKLFGVRLEVEGERRRKTKRAEE----TAF 240
           LASMK KC+ L DLVA Y N S+KEDED RPKLFGVRL  EGER RK KR EE    T  
Sbjct: 181 LASMKNKCKGLFDLVATYENISKKEDEDVRPKLFGVRL--EGERERKIKRGEEISETTTI 240

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BLAST of Carg24984 vs. NCBI nr
Match: POE48517.1 (heat stress transcription factor b-3 [Quercus suber])

HSP 1 Score: 273.9 bits (699), Expect = 5.3e-70
Identity = 155/219 (70.78%), Postives = 170/219 (77.63%), Query Frame = 0


Query: 28  MKKSTPPPFLVKTYTVVEDPATDKVISWNGEGTAFVVWQTAEFAKDVLPKLFKHCNFSSF 87
           ++KS+PPPFL+KTY +VEDP TD VISWN EGTAFVVWQ AEFA+D+LP LFKH NFSSF
Sbjct: 82  VRKSSPPPFLLKTYMLVEDPVTDDVISWNVEGTAFVVWQPAEFARDLLPTLFKHSNFSSF 141

Query: 88  VRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLCEIRRRKAWTNKQQ----HEVQXXXXXX 147
           VRQLNTYGFRKV T RWEFCND FRKGEKE LCEIRRRKAW+NKQQ     +        
Sbjct: 142 VRQLNTYGFRKVATNRWEFCNDMFRKGEKELLCEIRRRKAWSNKQQPVAPTQSTPQEFDE 201

Query: 148 XXXXXXXXXXXXXXXLMDENKRLKKENGALSLELASMKGKCRELLDLVAKYGNGSRKEDE 207
              XXXXXXXX    L+DENKRLKKENG L+ EL SMK KC+ELLDLVAKY + S KE+E
Sbjct: 202 DQRXXXXXXXXGFSTLIDENKRLKKENGVLNSELLSMKKKCKELLDLVAKYAH-SEKEEE 261

Query: 208 DERPKLFGVRLEVEGERRRKTKRA---EETAFLPSQSCK 240
           DERPKLFGVRLEV GER  K KRA   E  + L SQSCK
Sbjct: 262 DERPKLFGVRLEVPGEREMKRKRAEIRESASILLSQSCK 299

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BLAST of Carg24984 vs. TAIR10
Match: AT2G41690.1 (heat shock transcription factor B3)

HSP 1 Score: 193.7 bits (491), Expect = 1.3e-49
Identity = 128/234 (54.70%), Postives = 151/234 (64.53%), Query Frame = 0


Query: 6   SNNNGDQKGLRLPSPPRTVESMMKKSTPPPFLVKTYTVVEDPATDKVISWNGEGTAFVVW 65
           ++N  D++ L L        S  +   PPPFLVKTY VVEDP TD VISWN  GT FVVW
Sbjct: 11  NDNVNDEERLPLEFMIGNSTSTAELQPPPPFLVKTYKVVEDPTTDGVISWNEYGTGFVVW 70

Query: 66  QTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLCEIRRR 125
           Q AEFA+D+LP LFKHCNFSSFVRQLNTYGFRKVTT RWEF N+ FRKG++E +  IRRR
Sbjct: 71  QPAEFARDLLPTLFKHCNFSSFVRQLNTYGFRKVTTIRWEFSNEMFRKGQRELMSNIRRR 130

Query: 126 KA--WT-NKQQHEV---------------QXXXXXXXXXXXXXXXXXXXXXLMDENKRLK 185
           K+  W+ NK  H+V                  XXXXXXXXXXXXXX     L+DENK LK
Sbjct: 131 KSQHWSHNKSNHQVVPTTTMVNQEGHQRIGIDXXXXXXXXXXXXXXFVYTALLDENKCLK 190

Query: 186 KENGALSLELASMKGKCRELLDLVAKY------GNGSRKEDEDERPKLFGVRLE 216
            EN  LS EL   K KC++L++LV +Y            ++EDE  KLFGV+LE
Sbjct: 191 NENELLSCELGKTKKKCKQLMELVERYRGEDEDATDESDDEEDEGLKLFGVKLE 244

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BLAST of Carg24984 vs. TAIR10
Match: AT1G46264.1 (heat shock transcription factor B4)

HSP 1 Score: 140.6 bits (353), Expect = 1.3e-33
Identity = 63/97 (64.95%), Postives = 75/97 (77.32%), Query Frame = 0


Query: 30  KSTPPPFLVKTYTVVEDPATDKVISWNGEGTAFVVWQTAEFAKDVLPKLFKHCNFSSFVR 89
           K+ P PFL KTY +V+DPATD V+SW  + T FVVW+  EFA+D+LP  FKH NFSSFVR
Sbjct: 29  KAVPAPFLTKTYQLVDDPATDHVVSWGDDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 88

Query: 90  QLNTYGFRKVTTTRWEFCNDKFRKGEKEKLCEIRRRK 127
           QLNTYGFRK+   RWEF N+ F++GEK  LCEI RRK
Sbjct: 89  QLNTYGFRKIVPDRWEFANEFFKRGEKHLLCEIHRRK 125

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BLAST of Carg24984 vs. TAIR10
Match: AT4G11660.1 (winged-helix DNA-binding transcription factor family protein)

HSP 1 Score: 140.6 bits (353), Expect = 1.3e-33
Identity = 65/98 (66.33%), Postives = 79/98 (80.61%), Query Frame = 0


Query: 29  KKSTPPPFLVKTYTVVEDPATDKVISWNGEGTAFVVWQTAEFAKDVLPKLFKHCNFSSFV 88
           ++S P PFL KTY +VEDP  D++ISWN +GT F+VW+ AEFA+D+LPK FKH NFSSFV
Sbjct: 53  QRSIPTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFKHNNFSSFV 112

Query: 89  RQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLCEIRRRK 127
           RQLNTYGFRKV   RWEF ND F++GEK  L +I+RRK
Sbjct: 113 RQLNTYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRK 150

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BLAST of Carg24984 vs. TAIR10
Match: AT4G36990.1 (heat shock factor 4)

HSP 1 Score: 136.7 bits (343), Expect = 1.8e-32
Identity = 62/100 (62.00%), Postives = 78/100 (78.00%), Query Frame = 0


Query: 23  TVESMMKKSTPPPFLVKTYTVVEDPATDKVISWNGEGTAFVVWQTAEFAKDVLPKLFKHC 82
           T  +  ++S P PFL KTY +V+D +TD V+SWN EGTAFVVW+TAEFAKD+LP+ FKH 
Sbjct: 2   TAVTAAQRSVPAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHN 61

Query: 83  NFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLCEI 123
           NFSSF+RQLNTYGFRK    +WEF ND FR+G ++ L +I
Sbjct: 62  NFSSFIRQLNTYGFRKTVPDKWEFANDYFRRGGEDLLTDI 101

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BLAST of Carg24984 vs. TAIR10
Match: AT4G17750.1 (heat shock factor 1)

HSP 1 Score: 133.7 bits (335), Expect = 1.6e-31
Identity = 65/124 (52.42%), Postives = 81/124 (65.32%), Query Frame = 0


Query: 4   GVSNNNGDQKGLRLPSPPRTVESMMKKSTPPPFLVKTYTVVEDPATDKVISWNGEGTAFV 63
           G   N G+      P  P     +   S PPPFL KTY +VEDPATD ++SW+    +F+
Sbjct: 21  GGGTNIGEAVTAPPPRNPHPATLLNANSLPPPFLSKTYDMVEDPATDAIVSWSPTNNSFI 80

Query: 64  VWQTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLCEIR 123
           VW   EF++D+LPK FKH NFSSFVRQLNTYGFRKV   RWEF N+ F +G+K  L +I 
Sbjct: 81  VWDPPEFSRDLLPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKKIS 140

Query: 124 RRKA 128
           RRK+
Sbjct: 141 RRKS 144

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BLAST of Carg24984 vs. Swiss-Prot
Match: sp|O22230|HSFB3_ARATH (Heat stress transcription factor B-3 OS=Arabidopsis thaliana OX=3702 GN=HSFB3 PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.3e-48
Identity = 128/234 (54.70%), Postives = 151/234 (64.53%), Query Frame = 0


Query: 6   SNNNGDQKGLRLPSPPRTVESMMKKSTPPPFLVKTYTVVEDPATDKVISWNGEGTAFVVW 65
           ++N  D++ L L        S  +   PPPFLVKTY VVEDP TD VISWN  GT FVVW
Sbjct: 11  NDNVNDEERLPLEFMIGNSTSTAELQPPPPFLVKTYKVVEDPTTDGVISWNEYGTGFVVW 70

Query: 66  QTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLCEIRRR 125
           Q AEFA+D+LP LFKHCNFSSFVRQLNTYGFRKVTT RWEF N+ FRKG++E +  IRRR
Sbjct: 71  QPAEFARDLLPTLFKHCNFSSFVRQLNTYGFRKVTTIRWEFSNEMFRKGQRELMSNIRRR 130

Query: 126 KA--WT-NKQQHEV---------------QXXXXXXXXXXXXXXXXXXXXXLMDENKRLK 185
           K+  W+ NK  H+V                  XXXXXXXXXXXXXX     L+DENK LK
Sbjct: 131 KSQHWSHNKSNHQVVPTTTMVNQEGHQRIGIDXXXXXXXXXXXXXXFVYTALLDENKCLK 190

Query: 186 KENGALSLELASMKGKCRELLDLVAKY------GNGSRKEDEDERPKLFGVRLE 216
            EN  LS EL   K KC++L++LV +Y            ++EDE  KLFGV+LE
Sbjct: 191 NENELLSCELGKTKKKCKQLMELVERYRGEDEDATDESDDEEDEGLKLFGVKLE 244

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BLAST of Carg24984 vs. Swiss-Prot
Match: sp|P22335|HSF24_SOLPE (Heat shock factor protein HSF24 OS=Solanum peruvianum OX=4082 GN=HSF24 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 6.9e-37
Identity = 86/190 (45.26%), Postives = 109/190 (57.37%), Query Frame = 0


Query: 27  MMKKSTPPPFLVKTYTVVEDPATDKVISWNGEGTAFVVWQTAEFAKDVLPKLFKHCNFSS 86
           M +++ P PFL+KTY +V+D ATD VISWN  GT FVVW+TAEFAKD+LPK FKH NFSS
Sbjct: 1   MSQRTAPAPFLLKTYQLVDDAATDDVISWNEIGTTFVVWKTAEFAKDLLPKYFKHNNFSS 60

Query: 87  FVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLCEIRRRKAWTN---------------- 146
           FVRQLNTYGFRK+   +WEF N+ F++G+KE L  IRRRK  T+                
Sbjct: 61  FVRQLNTYGFRKIVPDKWEFANENFKRGQKELLTAIRRRKTVTSTPAGGKSVAAGASASP 120

Query: 147 ------KQQHEVQXXXXXXXXXXXXXXXXXXXXXLMDENKRLKKENGALSLELASMKGKC 195
                        X                    L DEN++LKK+N  LS EL   K +C
Sbjct: 121 DNSXXXXXXXXXXXPDSKNPGSVDTPGKLSQFTDLSDENEKLKKDNQMLSSELVQAKKQC 180

[Close]

BLAST of Carg24984 vs. Swiss-Prot
Match: sp|Q652B0|HFB2C_ORYSJ (Heat stress transcription factor B-2c OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB2C PE=2 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 8.4e-35
Identity = 68/98 (69.39%), Postives = 79/98 (80.61%), Query Frame = 0


Query: 29  KKSTPPPFLVKTYTVVEDPATDKVISWNGEGTAFVVWQTAEFAKDVLPKLFKHCNFSSFV 88
           ++S P PFL KTY +VEDPA D VISWN +G+ FVVW+ AEFA+D+LPK FKH NFSSFV
Sbjct: 32  QRSLPTPFLTKTYQLVEDPAVDDVISWNEDGSTFVVWRPAEFARDLLPKYFKHNNFSSFV 91

Query: 89  RQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLCEIRRRK 127
           RQLNTYGFRK+   RWEF ND FR+GEK  LC+I RRK
Sbjct: 92  RQLNTYGFRKIVPDRWEFANDCFRRGEKRLLCDIHRRK 129

[Close]

BLAST of Carg24984 vs. Swiss-Prot
Match: sp|Q6Z9C8|HFB2B_ORYSJ (Heat stress transcription factor B-2b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB2B PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 7.1e-34
Identity = 66/94 (70.21%), Postives = 76/94 (80.85%), Query Frame = 0


Query: 33  PPPFLVKTYTVVEDPATDKVISWNGEGTAFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLN 92
           P PFL KTY +V+DPA D VISWN +G+ FVVW+ AEFA+D+LPK FKH NFSSFVRQLN
Sbjct: 46  PTPFLTKTYQLVDDPAVDDVISWNDDGSTFVVWRPAEFARDLLPKYFKHNNFSSFVRQLN 105

Query: 93  TYGFRKVTTTRWEFCNDKFRKGEKEKLCEIRRRK 127
           TYGFRK+   RWEF ND FR+GE+  LCEI RRK
Sbjct: 106 TYGFRKIVPDRWEFANDCFRRGERRLLCEIHRRK 139

[Close]

BLAST of Carg24984 vs. Swiss-Prot
Match: sp|Q7XRX3|HFB2A_ORYSJ (Heat stress transcription factor B-2a OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB2A PE=2 SV=2)

HSP 1 Score: 144.1 bits (362), Expect = 2.1e-33
Identity = 67/93 (72.04%), Postives = 76/93 (81.72%), Query Frame = 0


Query: 34  PPFLVKTYTVVEDPATDKVISWNGEGTAFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLNT 93
           PPFL KTY +VEDP+TD+ ISWN  GTAFVVW+ AEFA+D+LPK FKH NFSSFVRQLNT
Sbjct: 9   PPFLTKTYAMVEDPSTDETISWNDSGTAFVVWRPAEFARDLLPKHFKHSNFSSFVRQLNT 68

Query: 94  YGFRKVTTTRWEFCNDKFRKGEKEKLCEIRRRK 127
           YGF+KV   RWEF ND FR+GEK  L  I+RRK
Sbjct: 69  YGFKKVVADRWEFANDCFRRGEKHLLGGIQRRK 101

[Close]

BLAST of Carg24984 vs. TrEMBL
Match: tr|A0A2P4GWZ7|A0A2P4GWZ7_QUESU (Heat stress transcription factor b-3 OS=Quercus suber OX=58331 GN=CFP56_53105 PE=3 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 3.5e-70
Identity = 155/219 (70.78%), Postives = 170/219 (77.63%), Query Frame = 0


Query: 28  MKKSTPPPFLVKTYTVVEDPATDKVISWNGEGTAFVVWQTAEFAKDVLPKLFKHCNFSSF 87
           ++KS+PPPFL+KTY +VEDP TD VISWN EGTAFVVWQ AEFA+D+LP LFKH NFSSF
Sbjct: 82  VRKSSPPPFLLKTYMLVEDPVTDDVISWNVEGTAFVVWQPAEFARDLLPTLFKHSNFSSF 141

Query: 88  VRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLCEIRRRKAWTNKQQ----HEVQXXXXXX 147
           VRQLNTYGFRKV T RWEFCND FRKGEKE LCEIRRRKAW+NKQQ     +        
Sbjct: 142 VRQLNTYGFRKVATNRWEFCNDMFRKGEKELLCEIRRRKAWSNKQQPVAPTQSTPQEFDE 201

Query: 148 XXXXXXXXXXXXXXXLMDENKRLKKENGALSLELASMKGKCRELLDLVAKYGNGSRKEDE 207
              XXXXXXXX    L+DENKRLKKENG L+ EL SMK KC+ELLDLVAKY + S KE+E
Sbjct: 202 DQRXXXXXXXXGFSTLIDENKRLKKENGVLNSELLSMKKKCKELLDLVAKYAH-SEKEEE 261

Query: 208 DERPKLFGVRLEVEGERRRKTKRA---EETAFLPSQSCK 240
           DERPKLFGVRLEV GER  K KRA   E  + L SQSCK
Sbjct: 262 DERPKLFGVRLEVPGEREMKRKRAEIRESASILLSQSCK 299

[Close]

BLAST of Carg24984 vs. TrEMBL
Match: tr|A0A151TU31|A0A151TU31_CAJCA (Heat stress transcription factor B-3 OS=Cajanus cajan OX=3821 GN=KK1_009724 PE=3 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.1e-68
Identity = 161/235 (68.51%), Postives = 186/235 (79.15%), Query Frame = 0


Query: 8   NNGDQKGLRLPSPPRTVESMMKKSTPPPFLVKTYTVVEDPATDKVISWNGEGTAFVVWQT 67
           N+ D KGL             +K TPPPFL+KTY +VEDPATD V+SWN EGTAFVVWQ 
Sbjct: 6   NDHDNKGLL---------ECARKCTPPPFLMKTYMLVEDPATDDVVSWNREGTAFVVWQP 65

Query: 68  AEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLCEIRRRKA 127
           AEFA+D+LP LFKH NFSSFVRQLNTYGFRKV T+RWEF NDKF+KGE+E L EIRRRKA
Sbjct: 66  AEFARDLLPTLFKHSNFSSFVRQLNTYGFRKVATSRWEFFNDKFKKGERELLHEIRRRKA 125

Query: 128 WTNKQQHEVQXXXXXXXXXXXXXXXXXXXXXLMDENKRLKKENGALSLELASMKGKCREL 187
           WTNKQQ      XXXXXXXXXXXXXXXXXXX +DENKRLKKENG LS EL SMK KC+EL
Sbjct: 126 WTNKQQPNAPNQXXXXXXXXXXXXXXXXXXXXVDENKRLKKENGVLSSELTSMKRKCKEL 185

Query: 188 LDLVAKYGNGSRKEDEDERPKLFGVRLEVEGER---RRKTKRAEETAFLPSQSCK 240
           LDLVAKY + +++++EDERPKLFGVRL+V+G R   R +T+ +E  + + SQSCK
Sbjct: 186 LDLVAKYSSHAKEKEEDERPKLFGVRLDVQGGREMKRNRTEMSESASIMLSQSCK 231

[Close]

BLAST of Carg24984 vs. TrEMBL
Match: tr|A0A2N9IBC9|A0A2N9IBC9_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS51098 PE=3 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 9.6e-68
Identity = 160/219 (73.06%), Postives = 178/219 (81.28%), Query Frame = 0


Query: 28  MKKSTPPPFLVKTYTVVEDPATDKVISWNGEGTAFVVWQTAEFAKDVLPKLFKHCNFSSF 87
           ++KS+PPPFL+KTY +VEDP TD VISWN EGTAFVVWQ AEFA+D+LP LFKH NFSSF
Sbjct: 13  VRKSSPPPFLLKTYMLVEDPVTDDVISWNVEGTAFVVWQPAEFARDLLPTLFKHSNFSSF 72

Query: 88  VRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLCEIRRRKAWTNKQQ----HEVQXXXXXX 147
           VRQLNTYGFRKV T+RWEFCND FRKG K+ LCEIRRRKAW NKQQ     +    XXXX
Sbjct: 73  VRQLNTYGFRKVATSRWEFCNDMFRKGGKDLLCEIRRRKAWANKQQPVAPTQSTPQXXXX 132

Query: 148 XXXXXXXXXXXXXXXLMDENKRLKKENGALSLELASMKGKCRELLDLVAKYGNGSRKEDE 207
           XXXXXXXXXXXXX  L+DENKRLKKENG LS EL+S+K KC+ELLDLVAKY +     +E
Sbjct: 133 XXXXXXXXXXXXXSTLIDENKRLKKENGVLSSELSSIKRKCKELLDLVAKYAH----SEE 192

Query: 208 DERPKLFGVRLEVEGERRRKTKRAEETA---FLPSQSCK 240
           DERPKLFGVRLEV+GER  K KRAE +A    L SQSCK
Sbjct: 193 DERPKLFGVRLEVQGEREMKRKRAEISASASILLSQSCK 227

[Close]

BLAST of Carg24984 vs. TrEMBL
Match: tr|A0A2C9VFG9|A0A2C9VFG9_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_08G051600 PE=3 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 2.1e-67
Identity = 159/222 (71.62%), Postives = 178/222 (80.18%), Query Frame = 0


Query: 28  MKKSTPPPFLVKTYTVVEDPATDKVISWNGEGTAFVVWQTAEFAKDVLPKLFKHCNFSSF 87
           ++KSTPPPFL+KTY +VEDPATD+VISWNG+GT FVVWQ AEFA+D+LP LFKH NFSSF
Sbjct: 19  VRKSTPPPFLLKTYMLVEDPATDQVISWNGDGTGFVVWQPAEFARDLLPTLFKHSNFSSF 78

Query: 88  VRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLCEIRRRKAWTNKQQHEV----QXXXXXX 147
           VRQLNTYGFRKVTT+RWEFCND FRK E+E LC+IRRRKAW+NKQQ  V        XXX
Sbjct: 79  VRQLNTYGFRKVTTSRWEFCNDMFRKAERELLCQIRRRKAWSNKQQPTVPIQTTPQDXXX 138

Query: 148 XXXXXXXXXXXXXXXLMDENKRLKKENGALSLELASMKGKCRELLDLVAKYGNGSRKE-- 207
           XXXXXXXXXXXXXX L+DENKRLKKENG LS EL  MK KC+ELLDLVAKY +  ++E  
Sbjct: 139 XXXXXXXXXXXXXXILIDENKRLKKENGVLSSELTGMKRKCKELLDLVAKYAHFEKEEXX 198

Query: 208 -DEDERPKLFGVRLEVEGERRRKTKRA---EETAFLPSQSCK 240
              D+RPKLFGVRLEV G+R  K KRA   E    L SQSCK
Sbjct: 199 XXSDKRPKLFGVRLEVGGDREMKRKRAKIRECATVLLSQSCK 240

[Close]

BLAST of Carg24984 vs. TrEMBL
Match: tr|A0A251NAD0|A0A251NAD0_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G056700 PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 4.7e-67
Identity = 164/248 (66.13%), Postives = 190/248 (76.61%), Query Frame = 0


Query: 11  DQKGLRLPSPPRTVESMMKKSTPPPFLVKTYTVVEDPATDKVISWNGEGTAFVVWQTAEF 70
           DQKGL            ++KS+PPPFL+KTY +VEDP TD VISWN +G+AFVVWQ AEF
Sbjct: 6   DQKGLL---------EYVRKSSPPPFLLKTYMLVEDPTTDDVISWNDDGSAFVVWQPAEF 65

Query: 71  AKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKLCEIRRRKAWTN 130
           A+D+LP LFKH NFSSFVRQLNTYGFRKV+T+RWEFCNDKFRKGEK++LC+IRRRKAW +
Sbjct: 66  ARDLLPTLFKHSNFSSFVRQLNTYGFRKVSTSRWEFCNDKFRKGEKDQLCDIRRRKAWAS 125

Query: 131 KQQ-------------HEVQXXXXXXXXXXXXXXXXXXXXXLMDENKRLKKENGALSLEL 190
           KQQ              +  XXXXXXXXXXXXXXXXXX   L+DENKRLK+ENG LS EL
Sbjct: 126 KQQPINNIALNQAAQAMQNXXXXXXXXXXXXXXXXXXXYSSLVDENKRLKQENGVLSSEL 185

Query: 191 ASMKGKCRELLDLVAKYGNGSRKEDED--ER-PKLFGVRLEVEGERRRKTKRA---EETA 240
            SMK KC+ELLDLVAKYG+ + KE+++  ER PKLFGVRLEVEGE  RK KRA   E  +
Sbjct: 186 TSMKRKCKELLDLVAKYGDSAEKEEQENSERVPKLFGVRLEVEGETERKRKRAEISESAS 244

The following BLAST results are available for this feature:

BLAST of Carg24984 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022928625.1	3.5e-114	97.91	heat stress transcription factor B-3 [Cucurbita moschata]	[more]
XP_023544792.1	1.7e-113	97.51	heat stress transcription factor B-3 [Cucurbita pepo subsp. pepo]	[more]
XP_022967894.1	5.8e-109	94.56	heat stress transcription factor B-3 [Cucurbita maxima]	[more]
XP_004149402.1	1.3e-73	73.28	PREDICTED: heat stress transcription factor B-3 [Cucumis sativus]	[more]
POE48517.1	5.3e-70	70.78	heat stress transcription factor b-3 [Quercus suber]	[more]

BLAST of Carg24984 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G41690.1	1.3e-49	54.70	heat shock transcription factor B3	[more]
AT1G46264.1	1.3e-33	64.95	heat shock transcription factor B4	[more]
AT4G11660.1	1.3e-33	66.33	winged-helix DNA-binding transcription factor family protein	[more]
AT4G36990.1	1.8e-32	62.00	heat shock factor 4	[more]
AT4G17750.1	1.6e-31	52.42	heat shock factor 1	[more]

BLAST of Carg24984 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O22230\|HSFB3_ARATH	2.3e-48	54.70	Heat stress transcription factor B-3 OS=Arabidopsis thaliana OX=3702 GN=HSFB3 PE...	[more]
sp\|P22335\|HSF24_SOLPE	6.9e-37	45.26	Heat shock factor protein HSF24 OS=Solanum peruvianum OX=4082 GN=HSF24 PE=2 SV=1	[more]
sp\|Q652B0\|HFB2C_ORYSJ	8.4e-35	69.39	Heat stress transcription factor B-2c OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
sp\|Q6Z9C8\|HFB2B_ORYSJ	7.1e-34	70.21	Heat stress transcription factor B-2b OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
sp\|Q7XRX3\|HFB2A_ORYSJ	2.1e-33	72.04	Heat stress transcription factor B-2a OS=Oryza sativa subsp. japonica OX=39947 G...	[more]

BLAST of Carg24984 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A2P4GWZ7\|A0A2P4GWZ7_QUESU	3.5e-70	70.78	Heat stress transcription factor b-3 OS=Quercus suber OX=58331 GN=CFP56_53105 PE...	[more]
tr\|A0A151TU31\|A0A151TU31_CAJCA	1.1e-68	68.51	Heat stress transcription factor B-3 OS=Cajanus cajan OX=3821 GN=KK1_009724 PE=3...	[more]
tr\|A0A2N9IBC9\|A0A2N9IBC9_FAGSY	9.6e-68	73.06	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS51098 PE=3 SV=1	[more]
tr\|A0A2C9VFG9\|A0A2C9VFG9_MANES	2.1e-67	71.62	Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_08G051600 PE=3 SV=...	[more]
tr\|A0A251NAD0\|A0A251NAD0_PRUPE	4.7e-67	66.13	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G056700 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated

Vocabulary: **Molecular Function**
Term	Definition
GO:0043565	sequence-specific DNA binding
GO:0003700	transcription factor activity, sequence-specific DNA binding

Vocabulary: **Cellular Component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **INTERPRO**
Term	Definition
IPR036390	WH_DNA-bd_sf
IPR027725	HSF_fam
IPR036388	WH-like_DNA-bd_sf
IPR000232	HSF_DNA-bd

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005667	transcription factor complex
molecular_function	GO:0043565	sequence-specific DNA binding
molecular_function	GO:0003700	transcription factor activity, sequence-specific DNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg24984-RA	Carg24984-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 156..183
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 218..239
None	No IPR available	PANTHER	PTHR10015:SF266	TRANSCRIPTION FACTOR SKN7	coord: 25..202
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PRINTS	PR00056	HSFDOMAIN	coord: 36..59 score: 47.5 coord: 74..86 score: 67.91 coord: 87..99 score: 87.03
IPR000232	Heat shock factor (HSF)-type, DNA-binding	SMART	SM00415	hsfneu3	coord: 32..125 e-value: 3.1E-52 score: 189.5
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PFAM	PF00447	HSF_DNA-bind	coord: 36..125 e-value: 3.2E-29 score: 101.3
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PROSITE	PS00434	HSF_DOMAIN	coord: 75..99
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	G3DSA:1.10.10.10		coord: 28..127 e-value: 1.3E-36 score: 127.1
IPR027725	Heat shock transcription factor family	PANTHER	PTHR10015	HEAT SHOCK TRANSCRIPTION FACTOR	coord: 25..202
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	SSF46785	"Winged helix" DNA-binding domain	coord: 32..125

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg24984	ClCG04G003410	Watermelon (Charleston Gray)	carwcgB0509
Carg24984	CmaCh03G000560	Cucurbita maxima (Rimu)	carcmaB0735
Carg24984	CmoCh03G000560	Cucurbita moschata (Rifu)	carcmoB0712
Carg24984	Cp4.1LG10g11490	Cucurbita pepo (Zucchini)	carcpeB0742
Carg24984	Cla97C04G070500	Watermelon (97103) v2	carwmbB0550

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None