Carg24203 (gene) Silver-seed gourd

NameCarg24203
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionB3 domain-containing protein
LocationCucurbita_argyrosperma_scaffold_225 : 214123 .. 214833 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAACTGAGATGCGAAAGCAGATCGAGGACATGCATGGATACAATCTCCAATTGGTGATTCATAAGAGATTAATAGAAGCTGATATGAACGAGAAAGGGCGTTTGTTTTTTCGAATCAAGAAAATGTTCTTCGATTTCCTCACCCCAAATGAGAAGGAGCTCTTGAATGATGCAAACGGTGTCATGGAAGTCAAGATTGTTGATGAGGCTCAAAGGGTGAGCCCCATGTTCTTGACCAAAGGCAAGATTAAAGAACAGGATGTTTATTTTTTTACCACTCATTGGAATTCTTTTGTCGACAAAAATTGCTTGAAATTGGGCCACCTTATCCAACTTTGGAGTTTCAGAAGAGTTCGACTCGTAGACATCGACAACGAAATATTCACCTCTGATCTTTACTTCGCCTTGAATACCGTTGATGGTCGATGTATTCTTTAGAAGTGTTCTTGCGTAAATAATGGATAGGGTCAGTAGTTTTCTTTAGATGTTTTACCATTCGTAGATGTTTTTTTTTCTGTGTTTCTTGCATAGACTCCGTAGGTTTTTATAGAGTTTTTTCGTATGTTCCAGTTCAAGAAATTATTCATTCTTAAATAAATGCGAGGATTGGTAGTTTAATAGTTTTCACGTTTTGGATCTTGTTTATTTATTATATGTTTTCAATATATTTATTATATGTTTTCAATATTTTTAGGGGTGCTCGAGTAA

mRNA sequence

ATGCCAACTGAGATGCGAAAGCAGATCGAGGACATGCATGGATACAATCTCCAATTGGTGATTCATAAGAGATTAATAGAAGCTGATATGAACGAGAAAGGGCGTTTGTTTTTTCGAATCAAGAAAATGTTCTTCGATTTCCTCACCCCAAATGAGAAGGAGCTCTTGAATGATGCAAACGGTGTCATGGAAGTCAAGATTGTTGATGAGGCTCAAAGGGTGAGCCCCATGTTCTTGACCAAAGGCAAGATTAAAGAACAGGATGTTTATTTTTTTACCACTCATTGGAATTCTTTTGTCGACAAAAATTGCTTGAAATTGGGCCACCTTATCCAACTTTGGAGTTTCAGAAGAGTTCGACTCGTAGACATCGACAACGAAATATTCACCTCTGATCTTTACTTCGCCTTGAATACCGTTGATGGTCGATGGGTGCTCGAGTAA

Coding sequence (CDS)

ATGCCAACTGAGATGCGAAAGCAGATCGAGGACATGCATGGATACAATCTCCAATTGGTGATTCATAAGAGATTAATAGAAGCTGATATGAACGAGAAAGGGCGTTTGTTTTTTCGAATCAAGAAAATGTTCTTCGATTTCCTCACCCCAAATGAGAAGGAGCTCTTGAATGATGCAAACGGTGTCATGGAAGTCAAGATTGTTGATGAGGCTCAAAGGGTGAGCCCCATGTTCTTGACCAAAGGCAAGATTAAAGAACAGGATGTTTATTTTTTTACCACTCATTGGAATTCTTTTGTCGACAAAAATTGCTTGAAATTGGGCCACCTTATCCAACTTTGGAGTTTCAGAAGAGTTCGACTCGTAGACATCGACAACGAAATATTCACCTCTGATCTTTACTTCGCCTTGAATACCGTTGATGGTCGATGGGTGCTCGAGTAA

Protein sequence

MPTEMRKQIEDMHGYNLQLVIHKRLIEADMNEKGRLFFRIKKMFFDFLTPNEKELLNDANGVMEVKIVDEAQRVSPMFLTKGKIKEQDVYFFTTHWNSFVDKNCLKLGHLIQLWSFRRVRLVDIDNEIFTSDLYFALNTVDGRWVLE
BLAST of Carg24203 vs. NCBI nr
Match: XP_022938317.1 (B3 domain-containing protein At2g31420-like [Cucurbita moschata])

HSP 1 Score: 296.2 bits (757), Expect = 6.1e-77
Identity = 143/146 (97.95%), Postives = 145/146 (99.32%), Query Frame = 0

Query: 1   MPTEMRKQIEDMHGYNLQLVIHKRLIEADMNEKGRLFFRIKKMFFDFLTPNEKELLNDAN 60
           MPTEMRKQIEDMHGYNLQLVIHKRLIEADMNEKGRLFFRIKKMFFDFLTPNEKELLNDAN
Sbjct: 1   MPTEMRKQIEDMHGYNLQLVIHKRLIEADMNEKGRLFFRIKKMFFDFLTPNEKELLNDAN 60

Query: 61  GVMEVKIVDEAQRVSPMFLTKGKIKEQDVYFFTTHWNSFVDKNCLKLGHLIQLWSFRRVR 120
           GVMEVKIVDEAQRVSPMFLTKGKIK+QDVYFFTTHWNSFVDKNCLKLGHLIQLWSFRRVR
Sbjct: 61  GVMEVKIVDEAQRVSPMFLTKGKIKDQDVYFFTTHWNSFVDKNCLKLGHLIQLWSFRRVR 120

Query: 121 LVDIDNEIFTSDLYFALNTVDGRWVL 147
           LVDIDNEIFTSDLYFALNTVDGR +L
Sbjct: 121 LVDIDNEIFTSDLYFALNTVDGRCIL 146

BLAST of Carg24203 vs. NCBI nr
Match: XP_022969589.1 (B3 domain-containing protein At2g31420-like [Cucurbita maxima])

HSP 1 Score: 257.3 bits (656), Expect = 3.2e-65
Identity = 124/143 (86.71%), Postives = 135/143 (94.41%), Query Frame = 0

Query: 5   MRKQIEDMHGYNLQLVIHKRLIEADMNEK-GRLFFRIKKMFFDFLTPNEKELLNDANGVM 64
           MRKQIEDMHGYNLQ+VI+K+LI+ DMN+K GR+FF+IK +FFDFLTP EKELLNDANGVM
Sbjct: 1   MRKQIEDMHGYNLQMVIYKKLIDIDMNKKDGRMFFQIKNVFFDFLTPKEKELLNDANGVM 60

Query: 65  EVKIVDEAQRVSPMFLTKGKIKEQDVYFFTTHWNSFVDKNCLKLGHLIQLWSFRRVRLVD 124
           EVKIVDEAQRVSPMFLTKGKIKE DVY FTTHWNSFVDKNCLK G+LIQLWSFRRVRLVD
Sbjct: 61  EVKIVDEAQRVSPMFLTKGKIKEHDVYLFTTHWNSFVDKNCLKFGNLIQLWSFRRVRLVD 120

Query: 125 IDNEIFTSDLYFALNTVDGRWVL 147
           IDNEIFTSDLYFALNT+DGR +L
Sbjct: 121 IDNEIFTSDLYFALNTMDGRCIL 143

BLAST of Carg24203 vs. NCBI nr
Match: XP_022938318.1 (B3 domain-containing protein At2g31420-like [Cucurbita moschata])

HSP 1 Score: 251.5 bits (641), Expect = 1.7e-63
Identity = 119/143 (83.22%), Postives = 135/143 (94.41%), Query Frame = 0

Query: 5   MRKQIEDMHGYNLQLVIHKRLIEADMNEK-GRLFFRIKKMFFDFLTPNEKELLNDANGVM 64
           MRK+IEDMHGYNLQ+VI+K+LI+ DMN+K GR+FF+IK +FFDFLTP EKE+LNDANGV+
Sbjct: 1   MRKKIEDMHGYNLQMVIYKKLIDIDMNKKDGRMFFQIKNIFFDFLTPKEKEILNDANGVI 60

Query: 65  EVKIVDEAQRVSPMFLTKGKIKEQDVYFFTTHWNSFVDKNCLKLGHLIQLWSFRRVRLVD 124
           EVKIVDEAQRVSPMFLTKGKI+E DVY FTTHWNSFVDKNCLK G+LIQLWSFRRVRL+D
Sbjct: 61  EVKIVDEAQRVSPMFLTKGKIEEHDVYLFTTHWNSFVDKNCLKFGNLIQLWSFRRVRLID 120

Query: 125 IDNEIFTSDLYFALNTVDGRWVL 147
           IDNEIFTSDLYFALNT+DGR +L
Sbjct: 121 IDNEIFTSDLYFALNTMDGRCIL 143

BLAST of Carg24203 vs. NCBI nr
Match: XP_022938320.1 (B3 domain-containing protein At1g05930-like [Cucurbita moschata])

HSP 1 Score: 247.3 bits (630), Expect = 3.3e-62
Identity = 120/147 (81.63%), Postives = 132/147 (89.80%), Query Frame = 0

Query: 1   MPTEMRKQIEDMHGYNLQLVIHKRLIEADMNEK-GRLFFRIKKMFFDFLTPNEKELLNDA 60
           MPT MRKQIE+M GYNLQLVIHK+LIE DMNEK GRLF  IKK+ FDF+TP+E++LLNDA
Sbjct: 24  MPTRMRKQIEEMQGYNLQLVIHKKLIETDMNEKDGRLFLIIKKILFDFITPDEEKLLNDA 83

Query: 61  NGVMEVKIVDEAQRVSPMFLTKGKIKEQDVYFFTTHWNSFVDKNCLKLGHLIQLWSFRRV 120
           NGVMEVKI+DE QRVSPMFLTKGKI+E D Y  TTHWNSFV KNCLK+GHLIQLWSFRRV
Sbjct: 84  NGVMEVKILDEGQRVSPMFLTKGKIQEHDAYCLTTHWNSFVAKNCLKIGHLIQLWSFRRV 143

Query: 121 RLVDIDNEIFTSDLYFALNTVDGRWVL 147
           RLVDI+NEIFTSDLYFALNT+DGR  L
Sbjct: 144 RLVDIENEIFTSDLYFALNTMDGRCFL 170

BLAST of Carg24203 vs. NCBI nr
Match: XP_022144152.1 (B3 domain-containing protein At2g31420-like [Momordica charantia])

HSP 1 Score: 96.7 bits (239), Expect = 7.1e-17
Identity = 54/126 (42.86%), Postives = 75/126 (59.52%), Query Frame = 0

Query: 1   MPTEMRKQIEDMHGYNLQLVIHKRLIEADMNEK-GRLFFRIKKMFFDFLTPNEKELL--- 60
           MP  +R +I ++ GY++Q+VI KRL + D+N   GRL   IKK+  DF T  EK+LL   
Sbjct: 52  MPAXLRDRIAELGGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLLNDFTTEEEKQLLDQQ 111

Query: 61  ----NDANGVMEVKIVDEAQRVSPMFLTKGKIKEQDVYFFTTHWNSFVDKNCLKLGHLIQ 119
               N+ NGV  + I+D   R S + L K KI  +++Y   + WNS   KN L+ G  IQ
Sbjct: 112 DEHQNNKNGVXAL-ILDPCLRESTIHLKKWKIGSREIYCLVSQWNSLAQKNSLESGDFIQ 171

BLAST of Carg24203 vs. TAIR10
Match: AT2G31420.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 45.8 bits (107), Expect = 2.6e-05
Identity = 34/125 (27.20%), Postives = 62/125 (49.60%), Query Frame = 0

Query: 1   MPTEMRKQIEDMHGYNLQLVIHKR-LIEADMNE-KGRLFFRIKKM-FFDFLTPNEKELLN 60
           +P  + K +   +GY+ +L+I +R L   D+ + +G L   + K+   DFLT +E   L+
Sbjct: 60  IPDWLVKVMRKENGYDPKLIIKRRVLCTTDLRKNQGCLSMHLSKLEKSDFLTEDETRFLD 119

Query: 61  D-----ANGVMEVKIVDEAQRVSPMFLTKGKIKEQDVYFFTTHWNSFVDKNCLKLGHLIQ 118
           +         ++V +VD       ++L K        Y  +  WN+ +DK   K+  +I+
Sbjct: 120 EDFLKAKRDGLKVFLVDPESDKHVVYLKKWNGNTVWKYVLSHGWNNVIDKKIFKVNDVIE 179

BLAST of Carg24203 vs. Swiss-Prot
Match: sp|Q9SIC7|Y2142_ARATH (B3 domain-containing protein At2g31420 OS=Arabidopsis thaliana OX=3702 GN=At2g31420 PE=3 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 4.7e-04
Identity = 34/125 (27.20%), Postives = 62/125 (49.60%), Query Frame = 0

Query: 1   MPTEMRKQIEDMHGYNLQLVIHKR-LIEADMNE-KGRLFFRIKKM-FFDFLTPNEKELLN 60
           +P  + K +   +GY+ +L+I +R L   D+ + +G L   + K+   DFLT +E   L+
Sbjct: 60  IPDWLVKVMRKENGYDPKLIIKRRVLCTTDLRKNQGCLSMHLSKLEKSDFLTEDETRFLD 119

Query: 61  D-----ANGVMEVKIVDEAQRVSPMFLTKGKIKEQDVYFFTTHWNSFVDKNCLKLGHLIQ 118
           +         ++V +VD       ++L K        Y  +  WN+ +DK   K+  +I+
Sbjct: 120 EDFLKAKRDGLKVFLVDPESDKHVVYLKKWNGNTVWKYVLSHGWNNVIDKKIFKVNDVIE 179

BLAST of Carg24203 vs. TrEMBL
Match: tr|A0A1S3CSS4|A0A1S3CSS4_CUCME (putative B3 domain-containing protein At3g24850 OS=Cucumis melo OX=3656 GN=LOC103504467 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 4.9e-14
Identity = 51/126 (40.48%), Postives = 74/126 (58.73%), Query Frame = 0

Query: 1   MPTEMRKQIEDMHGYNLQLVIHKRLIEADMNE-KGRLFFRIKKMFFDFLTPNEKELLN-- 60
           MPT MR +IE+M GY + LVI K+L + D+N+ +GRL    KK+ FDF T  E+ LL+  
Sbjct: 105 MPTTMRDRIEEMGGYEINLVIQKQLTDTDLNKNEGRLSMPKKKLAFDFTTEEERNLLSQQ 164

Query: 61  ---DANGVMEVKIVDEAQRVSPMFLTKGKI--KEQDVYFFTTHWNSFVDKNCLKLGHLIQ 119
              +  G+  + + +  + +  + L K KI     DVY   T WN+FV++  LK G  IQ
Sbjct: 165 ENKNKKGINVMIMNNMVEDIRSICLKKWKIGSGNGDVYCLMTQWNAFVEETGLKSGQHIQ 224

BLAST of Carg24203 vs. TrEMBL
Match: tr|A0A1S4E600|A0A1S4E600_CUCME (putative B3 domain-containing protein At3g24850 OS=Cucumis melo OX=3656 GN=LOC103504562 PE=4 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 3.2e-13
Identity = 52/142 (36.62%), Postives = 76/142 (53.52%), Query Frame = 0

Query: 1   MPTEMRKQIEDMHGYNLQLVIHKRLIEADMNEK-GRLFFRIKKMFFDFLTPNEKELLNDA 60
           MP  MR +I +M GY ++LVI K+L + D+N+  GRL    K++ FDF T  E +LL++ 
Sbjct: 127 MPVAMRDRILEMGGYEIRLVIQKQLTDTDLNKNHGRLSMNTKQLSFDFATEEETKLLSEQ 186

Query: 61  NGV----MEVKIVDEAQRVSPMFLTKGKIKEQDVYFFTTHWNSFVDKNCLKLGHLIQLWS 120
                  + V ++D+      + L K KI   +VY   T WN  V++   K G  IQ+WS
Sbjct: 187 ENKNKRGINVMMLDDVLEERMLCLKKWKIGSGEVYCLMTRWNLMVEERGFKGGEEIQVWS 246

Query: 121 FRRVRLVDIDNEIFTSDLYFAL 138
           FR+      D+E     L FAL
Sbjct: 247 FRK------DDEYEAHRLCFAL 262

BLAST of Carg24203 vs. TrEMBL
Match: tr|A0A1S3BCJ5|A0A1S3BCJ5_CUCME (B3 domain-containing protein At3g25182-like OS=Cucumis melo OX=3656 GN=LOC103488545 PE=4 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 3.2e-13
Identity = 56/153 (36.60%), Postives = 84/153 (54.90%), Query Frame = 0

Query: 1   MPTEMRKQIEDMHGYNLQLVIHKRLIEADMNEK-GRLFFRIKKMFFDFLTPNEKEL---- 60
           M   +R +I+++ G+++Q V  KRL + DMN+  GRL    KK+  DF +  EK+L    
Sbjct: 93  MSAMLRDRIDELDGFDIQYVTQKRLQDTDMNKNHGRLLIPFKKLRNDFASEEEKQLLKQH 152

Query: 61  ----LNDANGVMEVKIVDEAQRVSPMFLTKGKIKEQDVYFFTTHWNSFVDKNCLKLGHLI 120
               L + NG M V I+D   R   + L   K+ + D Y   + WNSFV +N L+ G  I
Sbjct: 153 DQEDLKNKNG-MTVLIIDPQLRNRHIELKMWKVGQHDSYCLMSKWNSFVQENSLESGDYI 212

Query: 121 QLWSFR--RVRLVDIDNEI-FTSDLYFALNTVD 142
           QLWSFR   + +V   +++   S+L FAL  +D
Sbjct: 213 QLWSFRSNNIFIVGHQSQVPVNSNLCFALVKLD 244

BLAST of Carg24203 vs. TrEMBL
Match: tr|A0A0A0KP87|A0A0A0KP87_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G544060 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 5.4e-13
Identity = 48/123 (39.02%), Postives = 68/123 (55.28%), Query Frame = 0

Query: 1   MPTEMRKQIEDMHGYNLQLVIHKRLIEADMNEK-GRLFFRIKKMFFDFLTPNEKELLNDA 60
           MP  MR +I +M GY + LVI K+L + D+N+  GRL    K++ FDF T  E +LL++ 
Sbjct: 124 MPVAMRDRILEMGGYEINLVIQKQLTDTDLNKNHGRLSMNTKQLSFDFATEEESKLLSEQ 183

Query: 61  NGV----MEVKIVDEAQRVSPMFLTKGKIKEQDVYFFTTHWNSFVDKNCLKLGHLIQLWS 119
                  + V I+D+      + L K KI    VY   T WN  V++  LK G  IQ+WS
Sbjct: 184 ENKNKMGINVMILDDVLEERMLCLKKWKIGSGYVYCLMTKWNLMVEERGLKSGEEIQVWS 243

BLAST of Carg24203 vs. TrEMBL
Match: tr|A0A0A0LRL4|A0A0A0LRL4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G365090 PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 9.2e-13
Identity = 48/125 (38.40%), Postives = 70/125 (56.00%), Query Frame = 0

Query: 1   MPTEMRKQIEDMHGYNLQLVIHKRLIEADMNEK-GRLFFRIKKMFFDFLTPNEKELLNDA 60
           MP  MR ++    GY +QLVI K+L E+D++   GRL    KK+  +F+   E+ +L + 
Sbjct: 105 MPASMRDRVVGKGGYEIQLVIQKQLEESDVSRNHGRLCLPAKKVKTEFVREEERNILEEE 164

Query: 61  NG-------VMEVKIVDEAQRVSPMFLTKGKIKEQDVYFFTTHWNSFVDKNCLKLGHLIQ 118
           N         +EV ++D++ R S M L K KI     Y    +WN FV++N L+ G  IQ
Sbjct: 165 NDGKKKNKKGLEVGVMDDSLRESSMCLKKWKIGSGKFYCLMKNWNCFVEENGLRSGDYIQ 224

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022938317.16.1e-7797.95B3 domain-containing protein At2g31420-like [Cucurbita moschata][more]
XP_022969589.13.2e-6586.71B3 domain-containing protein At2g31420-like [Cucurbita maxima][more]
XP_022938318.11.7e-6383.22B3 domain-containing protein At2g31420-like [Cucurbita moschata][more]
XP_022938320.13.3e-6281.63B3 domain-containing protein At1g05930-like [Cucurbita moschata][more]
XP_022144152.17.1e-1742.86B3 domain-containing protein At2g31420-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT2G31420.12.6e-0527.20Domain of unknown function (DUF313) [more]
Match NameE-valueIdentityDescription
sp|Q9SIC7|Y2142_ARATH4.7e-0427.20B3 domain-containing protein At2g31420 OS=Arabidopsis thaliana OX=3702 GN=At2g31... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3CSS4|A0A1S3CSS4_CUCME4.9e-1440.48putative B3 domain-containing protein At3g24850 OS=Cucumis melo OX=3656 GN=LOC10... [more]
tr|A0A1S4E600|A0A1S4E600_CUCME3.2e-1336.62putative B3 domain-containing protein At3g24850 OS=Cucumis melo OX=3656 GN=LOC10... [more]
tr|A0A1S3BCJ5|A0A1S3BCJ5_CUCME3.2e-1336.60B3 domain-containing protein At3g25182-like OS=Cucumis melo OX=3656 GN=LOC103488... [more]
tr|A0A0A0KP87|A0A0A0KP87_CUCSA5.4e-1339.02Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G544060 PE=4 SV=1[more]
tr|A0A0A0LRL4|A0A0A0LRL4_CUCSA9.2e-1338.40Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G365090 PE=4 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005508DUF313
IPR015300DNA-bd_pseudobarrel_sf
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg24203-RACarg24203-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015300DNA-binding pseudobarrel domain superfamilyGENE3DG3DSA:2.40.330.10coord: 11..128
e-value: 2.5E-9
score: 38.6
IPR015300DNA-binding pseudobarrel domain superfamilySUPERFAMILYSSF101936DNA-binding pseudobarrel domaincoord: 21..118
IPR005508Protein of unknown function DUF313PFAMPF03754DUF313coord: 2..102
e-value: 2.4E-8
score: 34.1
NoneNo IPR availablePANTHERPTHR31541FAMILY NOT NAMEDcoord: 1..140

The following gene(s) are paralogous to this gene:

None