Carg23486 (gene) Silver-seed gourd

NameCarg23486
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionTransmembrane emp24 domain-containing protein
LocationCucurbita_argyrosperma_scaffold_278 : 10101 .. 11901 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAGGACACCGAGAGAGCGGGGATTCGGATATCTGTTGGCGGGTCTTCTTCTGGCGAGCTTAATCGCCAGAATCGATTCGCTTTCGGTCACAGTGAACGAGGTTGAATGCATCCATGAATACGTATTGTACGAAGGCGACACCGTTTCTGGGAATTTTGTCGTCGTCGATCATGAAATTTTCTGGGGCGCCGATCATCCCGGCATTGATTTCACTGTAAGCCACATTGGTTCCATCATTAATCTGGTTTTTAATTCATGTTTGCATGACAATGCGTTTCGTTTCTGCCGATTTGTAGATTGATCCCGATTACGATTGGATCTTGGATTAGGGGAATGTGTGGTAGAGTTCGGTTTTTTATTTCCAATTTTGCTGGACAATCTTCTTTTCTCTGTTGCGAAATTGGTTTAGGAAAGAAGGGTAAATGTTGTTCGTTGATCTTGACTTATTAAAGAACCTGGAATTTTTTGCCCTTTTGTTAATATGGACGTATGGGGCTTGTTTGTTTGTAATTGTGATGATACTTTAGATTGATAATTGTAGTACATTTCTTGTGAGCTTCGAAATGATCTGTTGTTGCACTGTTTATGATCATTAGTTTCACATTTATAATCCTTCGGGCATAGCCACCTGACACCCAAATGGTGTTGTGAAATTGTTATTTCTTTGGTCATCATCGACTGGATATTAGGGTACCTGGAATCTCTTGAAGGATTTTTGTATCCATAATGAAATGTTGTTTTGAAGATATCCTAACGTGGGGTTGGTTTTGTGCAGGTTACATCTCCCGCAGGTAATGTGGTGCACTCGTTGAAGGAGACATCTGGTGGCAAGTTTGAGTTCAAGGCTTCTCGCAGTGGAATGTACAAATTCTGTTTTCGTAATCCCTACTCGACCACCGAGACAGTTTCTTTCTACATCCATGTTGGTCACATTCCAAACGAACATGACCTTGCTAAAGATGGTATTTCACTTTTGTGCTTAGAGGACTTGGTTGCTTTTATGAAGTTGACACTTCTCTCATTTTTCACCTTGTTGTGGTAATTTCTTGCAGAACACCTAGACCCTGTAAATGTTAAAATTGCAGAGCTTAGAGAGGCATTGGAATCGGTCACCGCCGAGCAGAAATATTTGAAAGCACGTGATGCACGCCACCGTCACAGTATGTGGTTTATTTTTTGCATGGTTTATATATATATAAGAAGCATATAAGTTAATCATAAACGAAGAAATACAGCTTAGGTGCAGGGGGAAGAGAGAACCCCCTCCCAAAGGGAATTGGTCGTAGGTATGACACCGAACGCGATTTCTAACTTTGTGACACAAGATGAATGTCTTTTCTTCTCAAGTGGTCCGACATAACTATCATGATTATGATTTGATCTTGATGTCTATGTGTGGGATTGTAACACACGATAGCTTAAGCATCAATCTTTTAATAATGCATATAGGATCAAGCGTTGAAACCACGAGCTGAACAATAATTAGTAGCATAGACTTGAATATTATCCACCAGCATTATATCTCATGAAACTACGTAGAAATTAGATTCTTTTTTGTTTCCAGTTTAATATCGATGTAGACAAGAAGTTTATTCCATACTGATTTGGCAATTGTTATAGGATTCTTATTGTTTTTGCTACCTTTGGTTGTTGTGAATGCAGCTAATGAGAGTACAAGAAAGCGTGTAATTTTCTACACAGTGGGAGAGTATTTGTTGCTCGCTGTTGCAAGCGGGCTTCAGGTCGTTTACATTCGTCGCCTCTTCAGCAAGTCGGTTGCATATAACCGGGTCTGA

mRNA sequence

ATGGAGAGGACACCGAGAGAGCGGGGATTCGGATATCTGTTGGCGGGTCTTCTTCTGGCGAGCTTAATCGCCAGAATCGATTCGCTTTCGGTCACAGTGAACGAGGTTGAATGCATCCATGAATACGTATTGTACGAAGGCGACACCGTTTCTGGGAATTTTGTCGTCGTCGATCATGAAATTTTCTGGGGCGCCGATCATCCCGGCATTGATTTCACTGTTACATCTCCCGCAGGTAATGTGGTGCACTCGTTGAAGGAGACATCTGGTGGCAAGTTTGAGTTCAAGGCTTCTCGCAGTGGAATGTACAAATTCTGTTTTCGTAATCCCTACTCGACCACCGAGACAGTTTCTTTCTACATCCATGTTGGTCACATTCCAAACGAACATGACCTTGCTAAAGATGAACACCTAGACCCTGTAAATGTTAAAATTGCAGAGCTTAGAGAGGCATTGGAATCGGTCACCGCCGAGCAGAAATATTTGAAAGCACGTGATGCACGCCACCGTCACACTAATGAGAGTACAAGAAAGCGTGTAATTTTCTACACAGTGGGAGAGTATTTGTTGCTCGCTGTTGCAAGCGGGCTTCAGGTCGTTTACATTCGTCGCCTCTTCAGCAAGTCGGTTGCATATAACCGGGTCTGA

Coding sequence (CDS)

ATGGAGAGGACACCGAGAGAGCGGGGATTCGGATATCTGTTGGCGGGTCTTCTTCTGGCGAGCTTAATCGCCAGAATCGATTCGCTTTCGGTCACAGTGAACGAGGTTGAATGCATCCATGAATACGTATTGTACGAAGGCGACACCGTTTCTGGGAATTTTGTCGTCGTCGATCATGAAATTTTCTGGGGCGCCGATCATCCCGGCATTGATTTCACTGTTACATCTCCCGCAGGTAATGTGGTGCACTCGTTGAAGGAGACATCTGGTGGCAAGTTTGAGTTCAAGGCTTCTCGCAGTGGAATGTACAAATTCTGTTTTCGTAATCCCTACTCGACCACCGAGACAGTTTCTTTCTACATCCATGTTGGTCACATTCCAAACGAACATGACCTTGCTAAAGATGAACACCTAGACCCTGTAAATGTTAAAATTGCAGAGCTTAGAGAGGCATTGGAATCGGTCACCGCCGAGCAGAAATATTTGAAAGCACGTGATGCACGCCACCGTCACACTAATGAGAGTACAAGAAAGCGTGTAATTTTCTACACAGTGGGAGAGTATTTGTTGCTCGCTGTTGCAAGCGGGCTTCAGGTCGTTTACATTCGTCGCCTCTTCAGCAAGTCGGTTGCATATAACCGGGTCTGA

Protein sequence

MERTPRERGFGYLLAGLLLASLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHEIFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMYKFCFRNPYSTTETVSFYIHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRVIFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV
BLAST of Carg23486 vs. NCBI nr
Match: XP_022955882.1 (transmembrane emp24 domain-containing protein p24beta3-like [Cucurbita moschata])

HSP 1 Score: 426.0 bits (1094), Expect = 7.5e-116
Identity = 213/215 (99.07%), Postives = 214/215 (99.53%), Query Frame = 0

Query: 1   MERTPRERGFGYLLAGLLLASLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MERTPRERGFGYLLAG LLASLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE
Sbjct: 1   MERTPRERGFGYLLAGFLLASLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMYKFCFRNPYSTTETVSFY 120
           IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMY+FCFRNPYSTTETVSFY
Sbjct: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMYRFCFRNPYSTTETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV
Sbjct: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV
Sbjct: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 215

BLAST of Carg23486 vs. NCBI nr
Match: XP_023527186.1 (transmembrane emp24 domain-containing protein p24beta3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 424.5 bits (1090), Expect = 2.2e-115
Identity = 212/215 (98.60%), Postives = 215/215 (100.00%), Query Frame = 0

Query: 1   MERTPRERGFGYLLAGLLLASLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MERTPRERGFGYLLAGLLLASLIARIDSLSVTV+EVECIHEYVLYEGDTVSGNFVVVDHE
Sbjct: 1   MERTPRERGFGYLLAGLLLASLIARIDSLSVTVSEVECIHEYVLYEGDTVSGNFVVVDHE 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMYKFCFRNPYSTTETVSFY 120
           IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMY+FCFRNPYSTTETVSFY
Sbjct: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMYRFCFRNPYSTTETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKR+
Sbjct: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRL 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV
Sbjct: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 215

BLAST of Carg23486 vs. NCBI nr
Match: XP_022980825.1 (transmembrane emp24 domain-containing protein p24beta3-like [Cucurbita maxima])

HSP 1 Score: 421.0 bits (1081), Expect = 2.4e-114
Identity = 210/215 (97.67%), Postives = 213/215 (99.07%), Query Frame = 0

Query: 1   MERTPRERGFGYLLAGLLLASLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MER PRERGFGYLL GLLLA+LIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE
Sbjct: 1   MERRPRERGFGYLLTGLLLATLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMYKFCFRNPYSTTETVSFY 120
           IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGM++FCFRNPYSTTETVSFY
Sbjct: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV
Sbjct: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV
Sbjct: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 215

BLAST of Carg23486 vs. NCBI nr
Match: XP_023538709.1 (transmembrane emp24 domain-containing protein p24beta3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 399.4 bits (1025), Expect = 7.5e-108
Identity = 199/215 (92.56%), Postives = 206/215 (95.81%), Query Frame = 0

Query: 1   MERTPRERGFGYLLAGLLLASLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MER  RE GFGYLLAGLLLASL A IDS+SVTVNE+EC++EYVLYEGDTVSGNFVVVDH+
Sbjct: 1   MERRLRETGFGYLLAGLLLASLFAGIDSISVTVNELECVYEYVLYEGDTVSGNFVVVDHD 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMYKFCFRNPYSTTETVSFY 120
           IFWGADHPGIDF+VTSPAGNVVHSLKETSG KFEFKA RSGMYKFCF NPYST ETVSFY
Sbjct: 61  IFWGADHPGIDFSVTSPAGNVVHSLKETSGDKFEFKAPRSGMYKFCFHNPYSTPETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNEHDLAKDEHLDP+NVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV
Sbjct: 121 IHVGHIPNEHDLAKDEHLDPINVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV
Sbjct: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 215

BLAST of Carg23486 vs. NCBI nr
Match: XP_022939384.1 (transmembrane emp24 domain-containing protein p24beta3-like [Cucurbita moschata])

HSP 1 Score: 396.4 bits (1017), Expect = 6.4e-107
Identity = 197/215 (91.63%), Postives = 204/215 (94.88%), Query Frame = 0

Query: 1   MERTPRERGFGYLLAGLLLASLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MER  RE GFGYLLAGLLL SL   IDS+SVTVNE+EC++EYVLYEGDTVSGNFVVVDH+
Sbjct: 1   MERRLRETGFGYLLAGLLLVSLFTGIDSISVTVNELECVYEYVLYEGDTVSGNFVVVDHD 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMYKFCFRNPYSTTETVSFY 120
           IFWGADHPGIDF+VTSPAGNVVHSLKETSG KFEFKA RSGMYKFCF NPYST ETVSFY
Sbjct: 61  IFWGADHPGIDFSVTSPAGNVVHSLKETSGDKFEFKAPRSGMYKFCFHNPYSTPETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNEHDLAKDEHLDP+NVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV
Sbjct: 121 IHVGHIPNEHDLAKDEHLDPINVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV
Sbjct: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 215

BLAST of Carg23486 vs. TAIR10
Match: AT3G22845.1 (emp24/gp25L/p24 family/GOLD family protein)

HSP 1 Score: 350.5 bits (898), Expect = 7.3e-97
Identity = 171/204 (83.82%), Postives = 187/204 (91.67%), Query Frame = 0

Query: 12  YLLAGLLLASLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHEIFWGADHPGID 71
           ++L GL+L + I +I SLSVTVN+ EC+ EYVLYEGDTVSGNFVVVDH+IFWG+DHPG+D
Sbjct: 11  FVLIGLILLNSINQISSLSVTVNDEECVQEYVLYEGDTVSGNFVVVDHDIFWGSDHPGLD 70

Query: 72  FTVTSPAGNVVHSLKETSGGKFEFKASRSGMYKFCFRNPYSTTETVSFYIHVGHIPNEHD 131
           FTVTSPAGN+V +LK TSG KFEFKA +SGMYKFCF NPYST ETVSFYIHVGHIPNEHD
Sbjct: 71  FTVTSPAGNIVQTLKGTSGDKFEFKAPKSGMYKFCFHNPYSTPETVSFYIHVGHIPNEHD 130

Query: 132 LAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRVIFYTVGEYLLL 191
           LAKDEHLDPVNVKIAELREALESV AEQKYLKARD RHRHTNESTRKRVIFYTVGEY+ L
Sbjct: 131 LAKDEHLDPVNVKIAELREALESVVAEQKYLKARDTRHRHTNESTRKRVIFYTVGEYIFL 190

Query: 192 AVASGLQVVYIRRLFSKSVAYNRV 216
           A ASGLQV+YIR+LFSKSVAYNRV
Sbjct: 191 AAASGLQVLYIRKLFSKSVAYNRV 214

BLAST of Carg23486 vs. TAIR10
Match: AT3G07680.1 (emp24/gp25L/p24 family/GOLD family protein)

HSP 1 Score: 120.2 bits (300), Expect = 1.6e-27
Identity = 63/183 (34.43%), Postives = 97/183 (53.01%), Query Frame = 0

Query: 33  VNEVECIHEYVLYEGDTVSGNFVVVDHEIFWGADHPGIDFTVTSPAGNVVHSLKETSGGK 92
           ++  EC      YEGDT+  +FVV+  +  W  +  G+D  +  P G  +H  +E    K
Sbjct: 26  IDREECFSHKAEYEGDTLHVSFVVIKSDSQWHFNEDGVDLVIHGPTGEQIHDFREQISAK 85

Query: 93  FEFKASRSGMYKFCFRNPYSTTETVSFYIHVGHIPNEHDLAKDEHLDPVNVKIAELREAL 152
            +F   + G+Y+FCF N     ET+ F + +GH       AKDEH  P+  +I++L EAL
Sbjct: 86  HDFVVQKKGVYRFCFTNKSPYHETIDFDVQLGHFAYYDQHAKDEHFTPLMEQISKLEEAL 145

Query: 153 ESVTAEQKYLKARDARHRHTNESTRKRVIFYTVGEYLLLAVASGLQVVYIRRLFSKSVAY 212
            ++  EQ +L+A+  R    NE+  KR +   + E   L  AS LQV  +RRLF + +  
Sbjct: 146 YNIQFEQHWLEAQTDRQAIVNENMSKRAVHKALFESFALIGASFLQVYLLRRLFERKLGM 205

Query: 213 NRV 216
           +RV
Sbjct: 206 SRV 208

BLAST of Carg23486 vs. TAIR10
Match: AT1G57620.1 (emp24/gp25L/p24 family/GOLD family protein)

HSP 1 Score: 76.6 bits (187), Expect = 2.0e-14
Identity = 57/205 (27.80%), Postives = 103/205 (50.24%), Query Frame = 0

Query: 13  LLAGLLLASLIARIDSLSVTVNEV--ECIHEYVLYEGDTVSGNFVVV--DHEIFWGADHP 72
           +L   L+ SL    +++ +TV     +C+ E +      V  +++V+  +H IF     P
Sbjct: 10  ILLSALIFSLSPICEAVWLTVPHTGSKCVSEEI-QSNVIVLADYLVISEEHSIF-----P 69

Query: 73  GIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMYKFCFRNPYSTTETVSFYIHV----G 132
            +   VT+P G V+H  + T+ G+F F    SG Y  CF     +     F I++    G
Sbjct: 70  TVSVKVTAPYGTVLHHRENTTNGQFAFTTQESGTYLACFEADAKSHGNKDFSINIDWKTG 129

Query: 133 HIPNEHD-LAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRVIFY 192
               + D +A+ E ++ V ++  +L  A+E++     YL+ R+A  R  +E T  RV +Y
Sbjct: 130 IAAKDWDSIARKEKIEGVELEFKKLEGAVEAIHENLIYLRNREAEMRIVSEKTNSRVAWY 189

Query: 193 TVGEYLLLAVASGLQVVYIRRLFSK 209
           ++    +  V SGLQ++Y+++ F K
Sbjct: 190 SIMSLGICIVVSGLQILYLKQYFEK 208

BLAST of Carg23486 vs. Swiss-Prot
Match: sp|Q9LIL4|P24B3_ARATH (Transmembrane emp24 domain-containing protein p24beta3 OS=Arabidopsis thaliana OX=3702 GN=At3g22845 PE=2 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 1.3e-95
Identity = 171/204 (83.82%), Postives = 187/204 (91.67%), Query Frame = 0

Query: 12  YLLAGLLLASLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHEIFWGADHPGID 71
           ++L GL+L + I +I SLSVTVN+ EC+ EYVLYEGDTVSGNFVVVDH+IFWG+DHPG+D
Sbjct: 11  FVLIGLILLNSINQISSLSVTVNDEECVQEYVLYEGDTVSGNFVVVDHDIFWGSDHPGLD 70

Query: 72  FTVTSPAGNVVHSLKETSGGKFEFKASRSGMYKFCFRNPYSTTETVSFYIHVGHIPNEHD 131
           FTVTSPAGN+V +LK TSG KFEFKA +SGMYKFCF NPYST ETVSFYIHVGHIPNEHD
Sbjct: 71  FTVTSPAGNIVQTLKGTSGDKFEFKAPKSGMYKFCFHNPYSTPETVSFYIHVGHIPNEHD 130

Query: 132 LAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRVIFYTVGEYLLL 191
           LAKDEHLDPVNVKIAELREALESV AEQKYLKARD RHRHTNESTRKRVIFYTVGEY+ L
Sbjct: 131 LAKDEHLDPVNVKIAELREALESVVAEQKYLKARDTRHRHTNESTRKRVIFYTVGEYIFL 190

Query: 192 AVASGLQVVYIRRLFSKSVAYNRV 216
           A ASGLQV+YIR+LFSKSVAYNRV
Sbjct: 191 AAASGLQVLYIRKLFSKSVAYNRV 214

BLAST of Carg23486 vs. Swiss-Prot
Match: sp|Q9S7M9|P24B2_ARATH (Transmembrane emp24 domain-containing protein p24beta2 OS=Arabidopsis thaliana OX=3702 GN=At3g07680 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.9e-26
Identity = 63/183 (34.43%), Postives = 97/183 (53.01%), Query Frame = 0

Query: 33  VNEVECIHEYVLYEGDTVSGNFVVVDHEIFWGADHPGIDFTVTSPAGNVVHSLKETSGGK 92
           ++  EC      YEGDT+  +FVV+  +  W  +  G+D  +  P G  +H  +E    K
Sbjct: 26  IDREECFSHKAEYEGDTLHVSFVVIKSDSQWHFNEDGVDLVIHGPTGEQIHDFREQISAK 85

Query: 93  FEFKASRSGMYKFCFRNPYSTTETVSFYIHVGHIPNEHDLAKDEHLDPVNVKIAELREAL 152
            +F   + G+Y+FCF N     ET+ F + +GH       AKDEH  P+  +I++L EAL
Sbjct: 86  HDFVVQKKGVYRFCFTNKSPYHETIDFDVQLGHFAYYDQHAKDEHFTPLMEQISKLEEAL 145

Query: 153 ESVTAEQKYLKARDARHRHTNESTRKRVIFYTVGEYLLLAVASGLQVVYIRRLFSKSVAY 212
            ++  EQ +L+A+  R    NE+  KR +   + E   L  AS LQV  +RRLF + +  
Sbjct: 146 YNIQFEQHWLEAQTDRQAIVNENMSKRAVHKALFESFALIGASFLQVYLLRRLFERKLGM 205

Query: 213 NRV 216
           +RV
Sbjct: 206 SRV 208

BLAST of Carg23486 vs. Swiss-Prot
Match: sp|Q769F9|TMEDA_DICDI (Transmembrane emp24 domain-containing protein A OS=Dictyostelium discoideum OX=44689 GN=empA PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 7.6e-19
Identity = 70/197 (35.53%), Postives = 105/197 (53.30%), Query Frame = 0

Query: 13  LLAGLLLAS-LIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHEIFWGADHPGID 72
           ++A L +AS  I    S  V+    ECI+E +      V  +F  +   I  G +   ID
Sbjct: 10  VIALLCIASNSIVESFSFKVSAKVEECIYEEI-----GVDLSFTAMFQVIQGGFN--DID 69

Query: 73  FTVTSPAGNVVHSLKETSGGKFEFKASRSGMYKFCFRNPYS--TTETVSFYIHVGHIPNE 132
           FT+ SP   +V+S +  S G    ++S +G+Y FCF N  S  T +TVSF + VG     
Sbjct: 70  FTIISPDKRIVYSGQRESEGTKTLRSSFAGVYSFCFSNKMSSLTDKTVSFILSVGESSPI 129

Query: 133 HDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRVIFYTVGEYL 192
            ++AK + L P+   I  L + + +V  EQ Y K R+A HR+T EST  RV++++V E  
Sbjct: 130 REIAKKKDLTPIERSIMTLSDGVIAVKNEQNYFKMREAAHRNTAESTNSRVLWWSVFEAF 189

Query: 193 LLAVASGLQVVYIRRLF 207
           +L   S  Q+ Y+RR F
Sbjct: 190 VLIALSIWQIYYLRRFF 199

BLAST of Carg23486 vs. Swiss-Prot
Match: sp|Q15363|TMED2_HUMAN (Transmembrane emp24 domain-containing protein 2 OS=Homo sapiens OX=9606 GN=TMED2 PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.2e-16
Identity = 61/194 (31.44%), Postives = 97/194 (50.00%), Query Frame = 0

Query: 17  LLLASLIARIDS--LSVTVNEVECIHEYVLYEGDTVSGNFVVVDHEIFWGADHPGIDFTV 76
           +LLA+L+A +    +S+  +  EC  E V       SG  + +  E+  G     ID  +
Sbjct: 9   VLLAALLATVSGYFVSIDAHAEECFFERV------TSGTKMGLIFEVAEGG-FLDIDVEI 68

Query: 77  TSPAGNVVHSLKETSGGKFEFKASRSGMYKFCFRNPYS--TTETVSFYIHVGHIPNEHDL 136
           T P    ++     S GK+ F A   G YKFCF N  S  T + V F I +G  P   D+
Sbjct: 69  TGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDM 128

Query: 137 AKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRVIFYTVGEYLLLA 196
             + H + +   I EL  A+ +V  EQ+Y++ R+  HR  N++T  RV+ ++  E L+L 
Sbjct: 129 ETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLV 188

Query: 197 VASGLQVVYIRRLF 207
             +  Q+ Y++R F
Sbjct: 189 AMTLGQIYYLKRFF 195

BLAST of Carg23486 vs. Swiss-Prot
Match: sp|P49020|TMED2_CRIGR (Transmembrane emp24 domain-containing protein 2 (Fragment) OS=Cricetulus griseus OX=10029 GN=TMED2 PE=1 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 3.5e-16
Identity = 61/194 (31.44%), Postives = 96/194 (49.48%), Query Frame = 0

Query: 17  LLLASLIARIDS--LSVTVNEVECIHEYVLYEGDTVSGNFVVVDHEIFWGADHPGIDFTV 76
           +LLA+L+A      +S+  +  EC  E V       SG  + +  E+  G     ID  +
Sbjct: 4   VLLAALLATASGYFVSIDAHAEECFFERV------TSGTKMGLIFEVAEGG-FLDIDVEI 63

Query: 77  TSPAGNVVHSLKETSGGKFEFKASRSGMYKFCFRNPYS--TTETVSFYIHVGHIPNEHDL 136
           T P    ++     S GK+ F A   G YKFCF N  S  T + V F I +G  P   D+
Sbjct: 64  TGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDM 123

Query: 137 AKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRVIFYTVGEYLLLA 196
             + H + +   I EL  A+ +V  EQ+Y++ R+  HR  N++T  RV+ ++  E L+L 
Sbjct: 124 ETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLV 183

Query: 197 VASGLQVVYIRRLF 207
             +  Q+ Y++R F
Sbjct: 184 AMTLGQIYYLKRFF 190

BLAST of Carg23486 vs. TrEMBL
Match: tr|A0A1S3AWG7|A0A1S3AWG7_CUCME (transmembrane emp24 domain-containing protein p24beta3 OS=Cucumis melo OX=3656 GN=LOC103483671 PE=3 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.8e-105
Identity = 194/215 (90.23%), Postives = 204/215 (94.88%), Query Frame = 0

Query: 1   MERTPRERGFGYLLAGLLLASLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MER  RE+G GYLLA LLL SLIA IDS+SVTVNE+EC++EYVLYEGD+VSGNFVVVDH+
Sbjct: 1   MERRVREKGLGYLLAALLLLSLIAGIDSISVTVNEIECVYEYVLYEGDSVSGNFVVVDHD 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMYKFCFRNPYSTTETVSFY 120
           IFWGADHPGIDFTVTSPAGNVVHSLKETSG KFEFKA RSGMYKFCF NPYST ETVSFY
Sbjct: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGDKFEFKAPRSGMYKFCFHNPYSTPETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNE+D AKDEHLDP+NVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV
Sbjct: 121 IHVGHIPNENDHAKDEHLDPINVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEYLLLAVASGLQV+YIRRLFSKSVAYNRV
Sbjct: 181 IFYTVGEYLLLAVASGLQVIYIRRLFSKSVAYNRV 215

BLAST of Carg23486 vs. TrEMBL
Match: tr|A0A0A0L4N3|A0A0A0L4N3_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G145770 PE=3 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 5.7e-104
Identity = 191/215 (88.84%), Postives = 202/215 (93.95%), Query Frame = 0

Query: 1   MERTPRERGFGYLLAGLLLASLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MER  RE+G G+LLA  LL SLIA IDS+SVTVNEVEC++EYVLYEGD+VSGNFVVVDH+
Sbjct: 1   MERRVREKGLGFLLAAFLLLSLIAGIDSISVTVNEVECVYEYVLYEGDSVSGNFVVVDHD 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMYKFCFRNPYSTTETVSFY 120
           IFWGADHPGI+FTVTSPAGNVVHSLKETSG KFEFKA RSGMYKFCF NPYST ETVSFY
Sbjct: 61  IFWGADHPGIEFTVTSPAGNVVHSLKETSGDKFEFKAPRSGMYKFCFHNPYSTPETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNEHD AKDEHLDP+NVKIAELREALESV +EQKYLKARDARHRHTNESTRKRV
Sbjct: 121 IHVGHIPNEHDHAKDEHLDPINVKIAELREALESVISEQKYLKARDARHRHTNESTRKRV 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEYLLLAVASGLQV+YIRRLFSKSVAYNRV
Sbjct: 181 IFYTVGEYLLLAVASGLQVIYIRRLFSKSVAYNRV 215

BLAST of Carg23486 vs. TrEMBL
Match: tr|A0A087HBR6|A0A087HBR6_ARAAL (Uncharacterized protein OS=Arabis alpina OX=50452 GN=AALP_AA3G261200 PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 8.2e-95
Identity = 175/204 (85.78%), Postives = 187/204 (91.67%), Query Frame = 0

Query: 12  YLLAGLLLASLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHEIFWGADHPGID 71
           ++L GL+L S I RI SLSVTVNE EC+ EYVLYEGDTVSGNFVVVDH+IFWG+DHPG+D
Sbjct: 11  FVLIGLILLSSIERISSLSVTVNEDECVQEYVLYEGDTVSGNFVVVDHDIFWGSDHPGLD 70

Query: 72  FTVTSPAGNVVHSLKETSGGKFEFKASRSGMYKFCFRNPYSTTETVSFYIHVGHIPNEHD 131
           FTVTSPAGN+V +LK TSG KFEFKA RSGMYKFCF NPYST ETVSFYIHVGHIPNEHD
Sbjct: 71  FTVTSPAGNIVQTLKGTSGDKFEFKAPRSGMYKFCFHNPYSTPETVSFYIHVGHIPNEHD 130

Query: 132 LAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRVIFYTVGEYLLL 191
           LAKDEHLDPVNVKIAELREALESV AEQKYLKARD RHRHTNESTRKRVIFYTVGEY+ L
Sbjct: 131 LAKDEHLDPVNVKIAELREALESVVAEQKYLKARDTRHRHTNESTRKRVIFYTVGEYMFL 190

Query: 192 AVASGLQVVYIRRLFSKSVAYNRV 216
           A ASGLQV+YIR+LFSKSVAYNRV
Sbjct: 191 AAASGLQVLYIRKLFSKSVAYNRV 214

BLAST of Carg23486 vs. TrEMBL
Match: tr|V4LAZ4|V4LAZ4_EUTSA (Uncharacterized protein OS=Eutrema salsugineum OX=72664 GN=EUTSA_v10021495mg PE=3 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.4e-94
Identity = 174/204 (85.29%), Postives = 187/204 (91.67%), Query Frame = 0

Query: 12  YLLAGLLLASLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHEIFWGADHPGID 71
           ++L GL+L S I RI SLSVTVN+ EC+ EYVLYEGDTVSGNFVVVDH+IFWG+DHPG+D
Sbjct: 14  FVLVGLILLSSIERISSLSVTVNDDECVQEYVLYEGDTVSGNFVVVDHDIFWGSDHPGLD 73

Query: 72  FTVTSPAGNVVHSLKETSGGKFEFKASRSGMYKFCFRNPYSTTETVSFYIHVGHIPNEHD 131
           FTVTSPAGN+V +LK TSG KFEFKA RSGMYKFCF NPYST ETVSFYIHVGHIPNEHD
Sbjct: 74  FTVTSPAGNIVQTLKGTSGDKFEFKAPRSGMYKFCFHNPYSTPETVSFYIHVGHIPNEHD 133

Query: 132 LAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRVIFYTVGEYLLL 191
           LAKDEHLDPVNVKIAELREALESV AEQKYLKARD RHRHTNESTRKRVIFYTVGEY+ L
Sbjct: 134 LAKDEHLDPVNVKIAELREALESVVAEQKYLKARDTRHRHTNESTRKRVIFYTVGEYIFL 193

Query: 192 AVASGLQVVYIRRLFSKSVAYNRV 216
           A ASGLQV+YIR+LFSKSVAYNRV
Sbjct: 194 AAASGLQVLYIRKLFSKSVAYNRV 217

BLAST of Carg23486 vs. TrEMBL
Match: tr|D7T744|D7T744_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_05s0020g04150 PE=3 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 2.4e-94
Identity = 178/216 (82.41%), Postives = 193/216 (89.35%), Query Frame = 0

Query: 1   MERTPRER-GFGYLLAGLLLASLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDH 60
           MER    R   G++   L   S I RI +LSVTVNEVEC++EYV+YEGDTVSGNFVVVDH
Sbjct: 1   MERRHTHRCATGFIFIALFF-SFIHRISALSVTVNEVECVYEYVIYEGDTVSGNFVVVDH 60

Query: 61  EIFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMYKFCFRNPYSTTETVSF 120
           +IFWG+DHPGIDFTVTSPAGN VH+LK TSG KFEFKA RSGMYKFCF+N Y+T ETVSF
Sbjct: 61  DIFWGSDHPGIDFTVTSPAGNTVHTLKGTSGDKFEFKAPRSGMYKFCFQNSYATPETVSF 120

Query: 121 YIHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKR 180
           YIHVGHIPNEH+LAKDEHLDP+NVKIAELREALESVTAEQKYLKARD RHRHTNESTRKR
Sbjct: 121 YIHVGHIPNEHNLAKDEHLDPINVKIAELREALESVTAEQKYLKARDTRHRHTNESTRKR 180

Query: 181 VIFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           VIFYT+GEYLLLA+ASGLQVVYIRRLFSKSVAYNRV
Sbjct: 181 VIFYTLGEYLLLALASGLQVVYIRRLFSKSVAYNRV 215

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022955882.17.5e-11699.07transmembrane emp24 domain-containing protein p24beta3-like [Cucurbita moschata][more]
XP_023527186.12.2e-11598.60transmembrane emp24 domain-containing protein p24beta3-like [Cucurbita pepo subs... [more]
XP_022980825.12.4e-11497.67transmembrane emp24 domain-containing protein p24beta3-like [Cucurbita maxima][more]
XP_023538709.17.5e-10892.56transmembrane emp24 domain-containing protein p24beta3-like [Cucurbita pepo subs... [more]
XP_022939384.16.4e-10791.63transmembrane emp24 domain-containing protein p24beta3-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G22845.17.3e-9783.82emp24/gp25L/p24 family/GOLD family protein[more]
AT3G07680.11.6e-2734.43emp24/gp25L/p24 family/GOLD family protein[more]
AT1G57620.12.0e-1427.80emp24/gp25L/p24 family/GOLD family protein[more]
Match NameE-valueIdentityDescription
sp|Q9LIL4|P24B3_ARATH1.3e-9583.82Transmembrane emp24 domain-containing protein p24beta3 OS=Arabidopsis thaliana O... [more]
sp|Q9S7M9|P24B2_ARATH2.9e-2634.43Transmembrane emp24 domain-containing protein p24beta2 OS=Arabidopsis thaliana O... [more]
sp|Q769F9|TMEDA_DICDI7.6e-1935.53Transmembrane emp24 domain-containing protein A OS=Dictyostelium discoideum OX=4... [more]
sp|Q15363|TMED2_HUMAN1.2e-1631.44Transmembrane emp24 domain-containing protein 2 OS=Homo sapiens OX=9606 GN=TMED2... [more]
sp|P49020|TMED2_CRIGR3.5e-1631.44Transmembrane emp24 domain-containing protein 2 (Fragment) OS=Cricetulus griseus... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3AWG7|A0A1S3AWG7_CUCME1.8e-10590.23transmembrane emp24 domain-containing protein p24beta3 OS=Cucumis melo OX=3656 G... [more]
tr|A0A0A0L4N3|A0A0A0L4N3_CUCSA5.7e-10488.84Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G145770 PE=3 SV=1[more]
tr|A0A087HBR6|A0A087HBR6_ARAAL8.2e-9585.78Uncharacterized protein OS=Arabis alpina OX=50452 GN=AALP_AA3G261200 PE=3 SV=1[more]
tr|V4LAZ4|V4LAZ4_EUTSA1.4e-9485.29Uncharacterized protein OS=Eutrema salsugineum OX=72664 GN=EUTSA_v10021495mg PE=... [more]
tr|D7T744|D7T744_VITVI2.4e-9482.41Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_05s0020g04150 PE=3 SV=... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR036598GOLD_dom_sf
IPR015720TMP21-related
IPR009038GOLD_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg23486-RACarg23486-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 145..165
NoneNo IPR availablePANTHERPTHR22811:SF31RE49489Pcoord: 25..207
IPR009038GOLD domainSMARTSM01190EMP24_GP25L_2coord: 28..207
e-value: 1.2E-53
score: 194.2
IPR009038GOLD domainPFAMPF01105EMP24_GP25Lcoord: 33..206
e-value: 4.0E-43
score: 147.4
IPR009038GOLD domainPROSITEPS50866GOLDcoord: 36..123
score: 17.628
IPR015720TMP21-relatedPANTHERPTHR22811TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEINcoord: 25..207
IPR036598GOLD domain superfamilySUPERFAMILYSSF101576Supernatant protein factor (SPF), C-terminal domaincoord: 40..132