Carg22888 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg22888
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Calcium-binding EF hand family protein
Location	Cucurbita_argyrosperma_scaffold_124 : 60574 .. 61131 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGAGTCAATTCCCCGTTGTCAACTTGAAGCTCGCCCCGACGCTCCTCCTCGTCGATCGGATATCGATTTCCTCGATTCTATTCTCCTTCGTCGATCGAATACATGACCTTATTTCTCAACCTTGGTCACTATTTCATTCACCCCCCAAAGAAAAGGCTTCCCCACAAGGCAATTCCTTAGAATCTGAGGCCACCCAACACAAAAGCAGCTCAATCAGCAAAGAAGAAGTGAAATTTGTGATGGAAAAGCTTGAGTTTTTTTGCAGCGAGGAGGAAATTGAGGAATCGTGGCTTGGAGGTGAGGAAATAGGTGCCATTTTTGATGAGAGTGAGCCAAGCTTGGAGGAGTTGAAGCAGACATTTAATGTGTTCGATAAGAATAGAGATGGGTGTATTGATGCGGATGAGCTGCAGTCCGTGCTGGGGTTGCTTGTACCTAATCAAGGCGTGTTCATCCATGATTGCCATAGAATGATTGCAAGATTTGATCATAACAAAGATGGTAAAATTGATTTCAATGAGTTTGTTAAGTTTATGGAAATTGCCTTGTCTTGA

mRNA sequence

ATGCCGAGTCAATTCCCCGTTGTCAACTTGAAGCTCGCCCCGACGCTCCTCCTCGTCGATCGGATATCGATTTCCTCGATTCTATTCTCCTTCGTCGATCGAATACATGACCTTATTTCTCAACCTTGGTCACTATTTCATTCACCCCCCAAAGAAAAGGCTTCCCCACAAGGCAATTCCTTAGAATCTGAGGCCACCCAACACAAAAGCAGCTCAATCAGCAAAGAAGAAGTGAAATTTGTGATGGAAAAGCTTGAGTTTTTTTGCAGCGAGGAGGAAATTGAGGAATCGTGGCTTGGAGGTGAGGAAATAGGTGCCATTTTTGATGAGAGTGAGCCAAGCTTGGAGGAGTTGAAGCAGACATTTAATGTGTTCGATAAGAATAGAGATGGGTGTATTGATGCGGATGAGCTGCAGTCCGTGCTGGGGTTGCTTGTACCTAATCAAGGCGTGTTCATCCATGATTGCCATAGAATGATTGCAAGATTTGATCATAACAAAGATGGTAAAATTGATTTCAATGAGTTTGTTAAGTTTATGGAAATTGCCTTGTCTTGA

Coding sequence (CDS)

ATGCCGAGTCAATTCCCCGTTGTCAACTTGAAGCTCGCCCCGACGCTCCTCCTCGTCGATCGGATATCGATTTCCTCGATTCTATTCTCCTTCGTCGATCGAATACATGACCTTATTTCTCAACCTTGGTCACTATTTCATTCACCCCCCAAAGAAAAGGCTTCCCCACAAGGCAATTCCTTAGAATCTGAGGCCACCCAACACAAAAGCAGCTCAATCAGCAAAGAAGAAGTGAAATTTGTGATGGAAAAGCTTGAGTTTTTTTGCAGCGAGGAGGAAATTGAGGAATCGTGGCTTGGAGGTGAGGAAATAGGTGCCATTTTTGATGAGAGTGAGCCAAGCTTGGAGGAGTTGAAGCAGACATTTAATGTGTTCGATAAGAATAGAGATGGGTGTATTGATGCGGATGAGCTGCAGTCCGTGCTGGGGTTGCTTGTACCTAATCAAGGCGTGTTCATCCATGATTGCCATAGAATGATTGCAAGATTTGATCATAACAAAGATGGTAAAATTGATTTCAATGAGTTTGTTAAGTTTATGGAAATTGCCTTGTCTTGA

Protein sequence

MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNSLESEATQHKSSSISKEEVKFVMEKLEFFCSEEEIEESWLGGEEIGAIFDESEPSLEELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKFMEIALS

Homology

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BLAST of Carg22888 vs. NCBI nr
Match: XP_023003075.1 (probable calcium-binding protein CML30 [Cucurbita maxima])

HSP 1 Score: 358.6 bits (919), Expect = 1.3e-95
Identity = 180/185 (97.30%), Postives = 183/185 (98.92%), Query Frame = 0


Query: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60
           MPSQFP+VNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEK SPQGNS
Sbjct: 1   MPSQFPIVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKVSPQGNS 60

Query: 61  LESEATQHKSSSISKEEVKFVMEKLEFFCSEEEIEESWLGGEEIGAIFDESEPSLEELKQ 120
           LESEATQHK+SSISKEEVKFVMEKLEFFCS+EEIEESWLGGEEIGAIF ESEPSLEELKQ
Sbjct: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ 120

Query: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKFM 180
           TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKFM
Sbjct: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKFM 180

Query: 181 EIALS 186
           EIALS
Sbjct: 181 EIALS 185

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BLAST of Carg22888 vs. NCBI nr
Match: XP_023530007.1 (probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 357.1 bits (915), Expect = 3.7e-95
Identity = 179/185 (96.76%), Postives = 184/185 (99.46%), Query Frame = 0


Query: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60
           MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS
Sbjct: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60

Query: 61  LESEATQHKSSSISKEEVKFVMEKLEFFCSEEEIEESWLGGEEIGAIFDESEPSLEELKQ 120
           LESEATQHK+SSISKEEVKFVMEKLEFFCS+EEI+ESWLGGEEIGAIF ESEPSLEELKQ
Sbjct: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIDESWLGGEEIGAIFYESEPSLEELKQ 120

Query: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKFM 180
           TFNVFDKNRDGCIDADELQS+LGLLVPNQGVFIHDCHRMIAR+DHNKDGKIDFNEFVKFM
Sbjct: 121 TFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEFVKFM 180

Query: 181 EIALS 186
           EIALS
Sbjct: 181 EIALS 185

[Close]

BLAST of Carg22888 vs. NCBI nr
Match: XP_022927644.1 (probable calcium-binding protein CML30 [Cucurbita moschata])

HSP 1 Score: 356.7 bits (914), Expect = 4.8e-95
Identity = 181/185 (97.84%), Postives = 183/185 (98.92%), Query Frame = 0


Query: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60
           MPSQFPVVNLKLA TLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS
Sbjct: 1   MPSQFPVVNLKLALTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60

Query: 61  LESEATQHKSSSISKEEVKFVMEKLEFFCSEEEIEESWLGGEEIGAIFDESEPSLEELKQ 120
           LESEATQHK+SSISKEEVKFVMEKLEFFCS+EEIEESWLGGEEIGAIF ESEPSLEELKQ
Sbjct: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ 120

Query: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKFM 180
           TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKFM
Sbjct: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKFM 180

Query: 181 EIALS 186
           EIALS
Sbjct: 181 EIALS 185

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BLAST of Carg22888 vs. NCBI nr
Match: XP_023528818.1 (probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 198.7 bits (504), Expect = 1.7e-47
Identity = 110/189 (58.20%), Postives = 134/189 (70.90%), Query Frame = 0


Query: 6   PVVNLKLAPT-------LLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQG 65
           P +NL+L P+       L+ +DR SI      F D IHDLIS  +S F SPPKEKA+PQ 
Sbjct: 16  PTINLQLIPSTTTTTPPLVFIDRASI------FADTIHDLISLVFSFFQSPPKEKAAPQS 75

Query: 66  NSLESEATQHKSSSISKEEVKFVMEKLEFFCSEEEIEE--SWLGGEEIGAIFDESEPSLE 125
            + +SE  +     +S+EEVK VMEKLE +C+EEE +E     G +E+  +F+E+EPSLE
Sbjct: 76  RASQSEGKE--DWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLE 135

Query: 126 ELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEF 185
           ELKQTFNVFD+NRDG IDA EL +VLGLL  N  +FIHDC  MIARFDHN D KIDFNEF
Sbjct: 136 ELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEF 195

[Close]

BLAST of Carg22888 vs. NCBI nr
Match: XP_022983385.1 (probable calcium-binding protein CML30 [Cucurbita maxima])

HSP 1 Score: 194.5 bits (493), Expect = 3.2e-46
Identity = 110/191 (57.59%), Postives = 136/191 (71.20%), Query Frame = 0


Query: 6   PVVNLKLAPT--------LLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQ 65
           P +NL+L P+        L+ +DR SI      F D IHDL+S  +S F SPPKEKA+PQ
Sbjct: 6   PTINLQLIPSTTTMMPPPLVFIDRASI------FADTIHDLVSLAFSFFQSPPKEKAAPQ 65

Query: 66  GNSLESEATQHKSSSISKEEVKFVMEKLEFFCSEE---EIEESWLGGEEIGAIFDESEPS 125
             + +SE  +     +S+EEVK VMEKLE +C+EE   EI+E + G EE+  +F+E+EPS
Sbjct: 66  SRASQSEGKE--DWLMSREEVKSVMEKLELYCNEEGDDEIDECF-GREEVRGMFEENEPS 125

Query: 126 LEELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFN 185
           LEELKQTFNVFD+NRDG IDA EL +VLGLLV N  +FIHDC  MIARFD N D KIDFN
Sbjct: 126 LEELKQTFNVFDRNRDGFIDAHELHTVLGLLVSNNTIFIHDCKSMIARFDRNNDEKIDFN 185

[Close]

BLAST of Carg22888 vs. TAIR10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 112.1 bits (279), Expect = 3.7e-25
Identity = 57/109 (52.29%), Postives = 79/109 (72.48%), Query Frame = 0


Query: 73  ISKEEVKFVMEKLEFFCSEEEIEESWLGGEEIGAIFDESEPSLEELKQTFNVFDKNRDGC 132
           IS+EE + VM  L  F ++++++E +   +E+ ++F+E E SLEE+KQ F+VFD+N+DG 
Sbjct: 83  ISREEAEMVMRSLGLFYNDDQLQEQY-SAKEVSSLFEEKEASLEEVKQAFDVFDENKDGF 142

Query: 133 IDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKFME 182
           IDA ELQ VL +L   QG ++ +C  MI   D NKDGKIDFNEFVKFME
Sbjct: 143 IDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFME 190

[Close]

BLAST of Carg22888 vs. TAIR10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 97.4 bits (241), Expect = 9.5e-21
Identity = 53/110 (48.18%), Postives = 72/110 (65.45%), Query Frame = 0


Query: 73  ISKEEVKFVMEKLEFFCSEE-EIEESWLGGEEIGAIFDESEPSLEELKQTFNVFDKNRDG 132
           + +E+V  VM+ L     +E E  +     +E+  +F+E EPSLEE+KQ F+VFD+NRDG
Sbjct: 91  LCREDVGMVMKSLGLSTDQENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDENRDG 150

Query: 133 CIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKFME 182
            ID  +LQ VL +L   QG  + +C RMI  FD +KDG+IDF  FVKFME
Sbjct: 151 FIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFME 200

[Close]

BLAST of Carg22888 vs. TAIR10
Match: AT4G12860.1 (EF hand calcium-binding protein family)

HSP 1 Score: 62.8 bits (151), Expect = 2.6e-10
Identity = 38/121 (31.40%), Postives = 64/121 (52.89%), Query Frame = 0


Query: 73  ISKEEVKFVMEKLEFFCSEEEIEE----------SWLGGEEIGAIFD---ESEPSLEELK 132
           I+K E+K   + +     E EI E            +  +E G+++    E +   E+++
Sbjct: 21  IAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSLYQEMVEEKEEEEDMR 80

Query: 133 QTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKF 181
           + F VFD+N DG I  +EL+SVL  +   QG  + DC +MI++ D + DG ++F EF + 
Sbjct: 81  EAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDVDGDGMVNFKEFKQM 140

[Close]

BLAST of Carg22888 vs. TAIR10
Match: AT5G44460.1 (calmodulin like 43)

HSP 1 Score: 61.6 bits (148), Expect = 5.8e-10
Identity = 38/96 (39.58%), Postives = 54/96 (56.25%), Query Frame = 0


Query: 86  EFFCSEEEIEESWLGGEEIGAIFDESEPSLEELKQTFNVFDKNRDGCIDADELQSVLGLL 145
           +F    + ++ES+ GGE  G+  D S  S  +L++ FNVFD++ DG I A ELQ VL  L
Sbjct: 84  DFAALHKTLDESFFGGE--GSCCDGSPES--DLEEAFNVFDEDGDGFISAVELQKVLKKL 143

Query: 146 VPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKFME 182
              +   I    +MI   D N DG++DF EF   M+
Sbjct: 144 GLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFKNMMQ 175

[Close]

BLAST of Carg22888 vs. TAIR10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 57.8 bits (138), Expect = 8.4e-09
Identity = 29/66 (43.94%), Postives = 40/66 (60.61%), Query Frame = 0


Query: 117 ELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEF 176
           ELK+TF  FD +RDG I ADEL  V    + ++   + DC RMIA  D + DG + F EF
Sbjct: 143 ELKETFEFFDADRDGLISADELLRVFS-TIGDERCTLDDCKRMIADVDEDGDGFVCFTEF 202

Query: 177 VKFMEI 183
            + M++
Sbjct: 203 SRMMDL 207

[Close]

BLAST of Carg22888 vs. Swiss-Prot
Match: sp|Q9MBG5|CML45_ARATH (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 6.8e-24
Identity = 57/109 (52.29%), Postives = 79/109 (72.48%), Query Frame = 0


Query: 73  ISKEEVKFVMEKLEFFCSEEEIEESWLGGEEIGAIFDESEPSLEELKQTFNVFDKNRDGC 132
           IS+EE + VM  L  F ++++++E +   +E+ ++F+E E SLEE+KQ F+VFD+N+DG 
Sbjct: 83  ISREEAEMVMRSLGLFYNDDQLQEQY-SAKEVSSLFEEKEASLEEVKQAFDVFDENKDGF 142

Query: 133 IDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKFME 182
           IDA ELQ VL +L   QG ++ +C  MI   D NKDGKIDFNEFVKFME
Sbjct: 143 IDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFME 190

[Close]

BLAST of Carg22888 vs. Swiss-Prot
Match: sp|Q93Z27|CML46_ARATH (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.7e-19
Identity = 53/110 (48.18%), Postives = 72/110 (65.45%), Query Frame = 0


Query: 73  ISKEEVKFVMEKLEFFCSEE-EIEESWLGGEEIGAIFDESEPSLEELKQTFNVFDKNRDG 132
           + +E+V  VM+ L     +E E  +     +E+  +F+E EPSLEE+KQ F+VFD+NRDG
Sbjct: 91  LCREDVGMVMKSLGLSTDQENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDENRDG 150

Query: 133 CIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKFME 182
            ID  +LQ VL +L   QG  + +C RMI  FD +KDG+IDF  FVKFME
Sbjct: 151 FIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFME 200

[Close]

BLAST of Carg22888 vs. Swiss-Prot
Match: sp|Q2QVI1|CML28_ORYSJ (Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 GN=CML28 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 5.6e-10
Identity = 37/80 (46.25%), Postives = 47/80 (58.75%), Query Frame = 0


Query: 101 GEEIGAIFDESEPSLEELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMI 160
           G E GA  DE     E +++ FNVFD+N DG I  DEL+SVL  L    G    DC RMI
Sbjct: 87  GGEGGAPDDED----EGMREAFNVFDQNGDGFITVDELRSVLSSLGLKHGRTADDCRRMI 146

Query: 161 ARFDHNKDGKIDFNEFVKFM 181
           +  D + DG++DF EF + M
Sbjct: 147 SMVDADGDGRVDFKEFKQMM 162

[Close]

BLAST of Carg22888 vs. Swiss-Prot
Match: sp|Q9SU00|CML2_ARATH (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 4.7e-09
Identity = 38/121 (31.40%), Postives = 64/121 (52.89%), Query Frame = 0


Query: 73  ISKEEVKFVMEKLEFFCSEEEIEE----------SWLGGEEIGAIFD---ESEPSLEELK 132
           I+K E+K   + +     E EI E            +  +E G+++    E +   E+++
Sbjct: 21  IAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSLYQEMVEEKEEEEDMR 80

Query: 133 QTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKF 181
           + F VFD+N DG I  +EL+SVL  +   QG  + DC +MI++ D + DG ++F EF + 
Sbjct: 81  EAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDVDGDGMVNFKEFKQM 140

[Close]

BLAST of Carg22888 vs. Swiss-Prot
Match: sp|Q0DZP5|CML17_ORYSJ (Probable calcium-binding protein CML17 OS=Oryza sativa subsp. japonica OX=39947 GN=CML17 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.0e-08
Identity = 29/63 (46.03%), Postives = 40/63 (63.49%), Query Frame = 0


Query: 118 LKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFV 177
           +++ F+VFD+N DG I  DEL +VL  L   QG    DC RMI + D + DG++DF EF 
Sbjct: 93  MREAFDVFDRNGDGFITVDELGAVLASLGIKQGRTAEDCGRMIGQVDRDGDGRVDFLEFK 152

Query: 178 KFM 181
           + M
Sbjct: 153 QMM 155

[Close]

BLAST of Carg22888 vs. TrEMBL
Match: tr|A0A0A0LHQ5|A0A0A0LHQ5_CUCSA (Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G825010 PE=4 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 3.8e-32
Identity = 97/195 (49.74%), Postives = 117/195 (60.00%), Query Frame = 0


Query: 7   VVNLKLAPT-LLLVDRISISSILFSFVDRIHDLI-SQPWSLFHSPPKEKASPQGNSLESE 66
           + +++L P+  + VDRISI  ILF+F DRI +L+ S PW +     KEKA  Q N     
Sbjct: 19  IKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQPHSKEKALSQCNCSSKS 78

Query: 67  ------ATQHKSSSISKEEVKFVMEKLEFFCSEEE--------IEESWLGGEEIGAIFDE 126
                  TQ   SS+S+EEV+FVMEKLE F  EE         I ES    E I  +F+E
Sbjct: 79  GDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGES---DEMIRGMFEE 138

Query: 127 SEPSLEELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGK 186
           +EPSLEELKQTFNVFD+NRDG ID  EL  VL LL  N+G+FIHDC  MIAR        
Sbjct: 139 NEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARXXXXXXXX 198

[Close]

BLAST of Carg22888 vs. TrEMBL
Match: tr|A0A1S3B8I1|A0A1S3B8I1_CUCME (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 PE=4 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 4.8e-27
Identity = 93/192 (48.44%), Postives = 116/192 (60.42%), Query Frame = 0


Query: 7   VVNLKLAPT-LLLVDRISISSILFSFVDRIHDLI-SQP-WSLFHSP-PKEKASPQGN-SL 66
           V +++L P+ L+ VD IS   ILF+F  RIH+LI S P WS+  S   KEKA  Q N S 
Sbjct: 9   VKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSS 68

Query: 67  ESE----ATQHKSSSISKEEVKFVMEKLEFF-----CSEEEIEESWLGGEEIGAIFDESE 126
           +S+     T  ++ S+S+EEV+FVMEKLE F                  E I  +F+E+E
Sbjct: 69  QSDDKIIITTQQNCSMSREEVRFVMEKLELFWRXXXXXXXXXXXXGASDEIIRGMFEENE 128

Query: 127 PSLEELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKID 185
           PSLEELKQTFNVFD+N+DG ID  EL  VL LL  N+G+FIHDC  MIAR          
Sbjct: 129 PSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARXXXXXXXXXX 188

[Close]

BLAST of Carg22888 vs. TrEMBL
Match: tr|A0A1Q3B181|A0A1Q3B181_CEPFO (EF_hand_5 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_05236 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 7.2e-23
Identity = 70/171 (40.94%), Postives = 99/171 (57.89%), Query Frame = 0


Query: 19  VDRISISSILFSFVDRIHDLISQPWSLFHS-----PPKEKASPQGNSLESEATQHK--SS 78
           +D +  + + F +V  I  + S+ WS+ H+       K K         S     K   +
Sbjct: 26  LDDLFTNMVFFDWVSSIPKVCSRYWSVLHTQFHCYDLKLKDETDSRLEFSNCVNEKKDDA 85

Query: 79  SISKEEVKFVMEKLEFFCSEEEIE-ESWLGGEEIGAIFDESEPSLEELKQTFNVFDKNRD 138
           ++ + EV+ +M KL  FCS E  E   +LG  E+  +F+E EPSLEE+K+ F+VFD+NRD
Sbjct: 86  NLYRHEVEMLMGKLGLFCSPESEELPQFLGSNELSGLFEEKEPSLEEVKEAFDVFDENRD 145

Query: 139 GCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKFME 182
           G IDA ELQ VL +L   +G  + +C+RMI  FD N DG+I FNEFVK ME
Sbjct: 146 GFIDARELQRVLCILGCKEGSVLENCNRMIKTFDQNGDGRIGFNEFVKLME 196

[Close]

BLAST of Carg22888 vs. TrEMBL
Match: tr|A0A2N9ER50|A0A2N9ER50_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS5073 PE=4 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.2e-22
Identity = 62/119 (52.10%), Postives = 82/119 (68.91%), Query Frame = 0


Query: 65  ATQHK-SSSISKEEVKFVMEKLEFFCSEE-EIEESWLGGEEIGAIFDESEPSLEELKQTF 124
           +T++K   S+S+E+V+ VM++LE  CS E E  + W G  E+  +FDE EPSLEE+KQ F
Sbjct: 9   STENKDDGSLSREDVELVMKRLELSCSPEGEKLQEWFGSNELSGLFDEKEPSLEEVKQAF 68

Query: 125 NVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKFME 182
            VFD+NRDG I A ELQ VL  L   +G  + +C +MI  F+ N DG+IDFN FVKFME
Sbjct: 69  EVFDENRDGFIVAKELQRVLRNLGLKEGEDLENCEKMIRAFNKNGDGRIDFNGFVKFME 127

[Close]

BLAST of Carg22888 vs. TrEMBL
Match: tr|A0A151TC87|A0A151TC87_CAJCA (Putative calcium-binding protein CML45 OS=Cajanus cajan OX=3821 GN=KK1_019263 PE=4 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 4.7e-22
Identity = 66/133 (49.62%), Postives = 91/133 (68.42%), Query Frame = 0


Query: 51  KEKASPQGNSLESEATQHKSSSISKEEVKFVMEKLEFFCS--EEEIEESWLGGEEIGAIF 110
           K + S Q N  ES  T+    ++ ++EVK VM KL FFCS   EE++E + G +E+  +F
Sbjct: 73  KSEFSHQDN--ESNGTR-DCENLERDEVKMVMAKLGFFCSSESEELQEKY-GSKELSELF 132

Query: 111 DESEPSLEELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKD 170
           +E EPSLEE+KQ F+VFD+N+DG I A+ELQ VL +L   +   + +CH+MI  FD N+D
Sbjct: 133 EEQEPSLEEVKQAFDVFDENKDGFIVAEELQRVLCVLGLKEAAELENCHKMIRVFDTNQD 192

Query: 171 GKIDFNEFVKFME 182
           G+IDF EFVK ME
Sbjct: 193 GRIDFIEFVKIME 201

The following BLAST results are available for this feature:

BLAST of Carg22888 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023003075.1	1.3e-95	97.30	probable calcium-binding protein CML30 [Cucurbita maxima]	[more]
XP_023530007.1	3.7e-95	96.76	probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo]	[more]
XP_022927644.1	4.8e-95	97.84	probable calcium-binding protein CML30 [Cucurbita moschata]	[more]
XP_023528818.1	1.7e-47	58.20	probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo]	[more]
XP_022983385.1	3.2e-46	57.59	probable calcium-binding protein CML30 [Cucurbita maxima]	[more]

BLAST of Carg22888 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G29000.1	3.7e-25	52.29	Calcium-binding EF-hand family protein	[more]
AT5G39670.1	9.5e-21	48.18	Calcium-binding EF-hand family protein	[more]
AT4G12860.1	2.6e-10	31.40	EF hand calcium-binding protein family	[more]
AT5G44460.1	5.8e-10	39.58	calmodulin like 43	[more]
AT3G10190.1	8.4e-09	43.94	Calcium-binding EF-hand family protein	[more]

BLAST of Carg22888 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9MBG5\|CML45_ARATH	6.8e-24	52.29	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
sp\|Q93Z27\|CML46_ARATH	1.7e-19	48.18	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
sp\|Q2QVI1\|CML28_ORYSJ	5.6e-10	46.25	Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
sp\|Q9SU00\|CML2_ARATH	4.7e-09	31.40	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]
sp\|Q0DZP5\|CML17_ORYSJ	1.0e-08	46.03	Probable calcium-binding protein CML17 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Carg22888 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LHQ5\|A0A0A0LHQ5_CUCSA	3.8e-32	49.74	Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G82501...	[more]
tr\|A0A1S3B8I1\|A0A1S3B8I1_CUCME	4.8e-27	48.44	probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 P...	[more]
tr\|A0A1Q3B181\|A0A1Q3B181_CEPFO	7.2e-23	40.94	EF_hand_5 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v...	[more]
tr\|A0A2N9ER50\|A0A2N9ER50_FAGSY	1.2e-22	52.10	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS5073 PE=4 SV=1	[more]
tr\|A0A151TC87\|A0A151TC87_CAJCA	4.7e-22	49.62	Putative calcium-binding protein CML45 OS=Cajanus cajan OX=3821 GN=KK1_019263 PE...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS
IPR002048	EF_hand_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg22888-RA	Carg22888-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 155..183 e-value: 0.0023 score: 27.2 coord: 117..145 e-value: 2.4E-5 score: 33.7
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 116..181 e-value: 9.4E-10 score: 38.7
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 151..185 score: 11.11
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 113..148 score: 14.988
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 117..180 e-value: 2.4824E-15 score: 66.4173
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 60..144 e-value: 7.6E-9 score: 37.4
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 154..181 e-value: 1.4E-8 score: 36.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 53..68
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 49..70
None	No IPR available	PANTHER	PTHR45512:SF1	CALCIUM-BINDING PROTEIN CML30-RELATED	coord: 21..183
None	No IPR available	PANTHER	PTHR45512	FAMILY NOT NAMED	coord: 21..183
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 164..176
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 126..138
IPR011992	EF-hand domain pair	SUPERFAMILY	SSF47473	EF-hand	coord: 69..180

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg22888	Cla010322	Watermelon (97103) v1	carwmB0200
Carg22888	Csa5G636620	Cucumber (Chinese Long) v2	carcuB0239
Carg22888	ClCG09G018130	Watermelon (Charleston Gray)	carwcgB0206
Carg22888	CSPI05G29010	Wild cucumber (PI 183967)	carcpiB0244
Carg22888	Cucsa.158220	Cucumber (Gy14) v1	carcgyB0256
Carg22888	CmaCh11G010180	Cucurbita maxima (Rimu)	carcmaB0291
Carg22888	CmoCh11G010400	Cucurbita moschata (Rifu)	carcmoB0297
Carg22888	Lsi02G014760	Bottle gourd (USVL1VR-Ls)	carlsiB190
Carg22888	Cp4.1LG04g01570	Cucurbita pepo (Zucchini)	carcpeB0302
Carg22888	CsGy5G028420	Cucumber (Gy14) v2	carcgybB0227
Carg22888	MELO3C021998.2	Melon (DHL92) v3.6.1	carmedB0222
Carg22888	CsaV3_5G038130	Cucumber (Chinese Long) v3	carcucB0242
Carg22888	Cla97C09G179530	Watermelon (97103) v2	carwmbB0217
Carg22888	Bhi06G000320	Wax gourd	carwgoB0266

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg22888	Carg02244	Silver-seed gourd	carcarB249

The following block(s) are covering this gene:


Gene	Organism	Block
Carg22888	Watermelon (97103) v2	carwmbB0215
Carg22888	Wax gourd	carwgoB0265
Carg22888	Wax gourd	carwgoB0267
Carg22888	Silver-seed gourd	carcarB250
Carg22888	Silver-seed gourd	carcarB251
Carg22888	Cucumber (Gy14) v2	carcgybB0226
Carg22888	Cucumber (Gy14) v2	carcgybB0228
Carg22888	Cucumber (Gy14) v1	carcgyB0257
Carg22888	Cucumber (Gy14) v1	carcgyB0258
Carg22888	Cucurbita maxima (Rimu)	carcmaB0292
Carg22888	Cucurbita moschata (Rifu)	carcmoB0298
Carg22888	Cucurbita pepo (Zucchini)	carcpeB0301
Carg22888	Wild cucumber (PI 183967)	carcpiB0243
Carg22888	Wild cucumber (PI 183967)	carcpiB0245
Carg22888	Cucumber (Chinese Long) v2	carcuB0237
Carg22888	Cucumber (Chinese Long) v2	carcuB0238
Carg22888	Cucumber (Chinese Long) v3	carcucB0241
Carg22888	Cucumber (Chinese Long) v3	carcucB0243
Carg22888	Bottle gourd (USVL1VR-Ls)	carlsiB191
Carg22888	Melon (DHL92) v3.5.1	carmeB0217
Carg22888	Melon (DHL92) v3.5.1	carmeB0219
Carg22888	Melon (DHL92) v3.6.1	carmedB0223
Carg22888	Melon (DHL92) v3.6.1	carmedB0225
Carg22888	Watermelon (Charleston Gray)	carwcgB0207
Carg22888	Watermelon (Charleston Gray)	carwcgB0209
Carg22888	Watermelon (97103) v1	carwmB0201
Carg22888	Watermelon (97103) v1	carwmB0202