Carg21992 (gene) Silver-seed gourd

Overview
NameCarg21992
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptiontransmembrane ascorbate ferrireductase 1-like
LocationCucurbita_argyrosperma_scaffold_186 : 139942 .. 140843 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTTCAAACGAAACTTCAACATAATGGCGAGGCCAGTCACTTTGTTGGGTCATTTGGTGGCCATATCTATCGCAGTCTTGATTCTTGTATGGATCCTCCATTTCAGAGAAGGGGTTGCTTTCAAATCAGATAACAAGCTCAAGATTCTAAATGTACTTCCTTTCTTCTATAATTTTAATTAGCGGTTTCACAAGCAAAGGTGTGATGAATTTCTCATTCTTCTTGTTCTTCAGCTGCATGTGCTTTTAATGGTGATTGGACTCGTCCTATTTGGAGGGCAAGGTAAGGGAAATTACTAATGATTTGAAGTCATGAATAAATCTTAATAACATTTATGGTTGGGATATGGCGCAGCTATAATGGCATACAAGTCAATACCAGCAAAAAAATACTGGAGAAAAGTGGTGCATTTGGGGTTGCATGCAATAGCATTAGCTGCTGCAATTTTTGGGATTTGTATGAGTTTCAAGTTTCACCATGACATCCAGTTGACGAATCTTGTTTCTTTGCACTCTTGGTTTGGTTTGATCACCGTTTGCTTGTATGGTCTACAGGTTCGTCGTCCCAGCCATCTCCCTCTTTCCAGACTTTGATATCTTTTCTTTGCTAATTATTATGATGTTGATACGCACAGTGGGTAGTAGGCTTCTTTGCGTACTTCTTCCCCGGAGCAGAATCAAGGAGGAGAGCCAGCCTAATGCCATGGCACGTTTACTTTGGAATATTCGTCTTCTTAATGGCAACTTGCACTGCTGAATTAGGGCTGCTTCAAAGCTTGGACACTTGGTTTTTGTTGGGGCACACCCAAGAAAACCTTATCATCAACTTCACAGGTCTTCTCATTTTTCTCTATGCAGCCACGGTCGTTCTCACTGTCATTCTTCCACCCCTTTATTAG

mRNA sequence

ATGGCGTTCAAACGAAACTTCAACATAATGGCGAGGCCAGTCACTTTGTTGGGTCATTTGGTGGCCATATCTATCGCAGTCTTGATTCTTGTATGGATCCTCCATTTCAGAGAAGGGGTTGCTTTCAAATCAGATAACAAGCTCAAGATTCTAAATCTGCATGTGCTTTTAATGGTGATTGGACTCGTCCTATTTGGAGGGCAAGCTATAATGGCATACAAGTCAATACCAGCAAAAAAATACTGGAGAAAAGTGGTGCATTTGGGGTTGCATGCAATAGCATTAGCTGCTGCAATTTTTGGGATTTGTATGAGTTTCAAGTTTCACCATGACATCCAGTTGACGAATCTTGTTTCTTTGCACTCTTGGTTTGGTTTGATCACCGTTTGCTTGTATGGTCTACAGTGGGTAGTAGGCTTCTTTGCGTACTTCTTCCCCGGAGCAGAATCAAGGAGGAGAGCCAGCCTAATGCCATGGCACGTTTACTTTGGAATATTCGTCTTCTTAATGGCAACTTGCACTGCTGAATTAGGGCTGCTTCAAAGCTTGGACACTTGGTTTTTGTTGGGGCACACCCAAGAAAACCTTATCATCAACTTCACAGGTCTTCTCATTTTTCTCTATGCAGCCACGGTCGTTCTCACTGTCATTCTTCCACCCCTTTATTAG

Coding sequence (CDS)

ATGGCGTTCAAACGAAACTTCAACATAATGGCGAGGCCAGTCACTTTGTTGGGTCATTTGGTGGCCATATCTATCGCAGTCTTGATTCTTGTATGGATCCTCCATTTCAGAGAAGGGGTTGCTTTCAAATCAGATAACAAGCTCAAGATTCTAAATCTGCATGTGCTTTTAATGGTGATTGGACTCGTCCTATTTGGAGGGCAAGCTATAATGGCATACAAGTCAATACCAGCAAAAAAATACTGGAGAAAAGTGGTGCATTTGGGGTTGCATGCAATAGCATTAGCTGCTGCAATTTTTGGGATTTGTATGAGTTTCAAGTTTCACCATGACATCCAGTTGACGAATCTTGTTTCTTTGCACTCTTGGTTTGGTTTGATCACCGTTTGCTTGTATGGTCTACAGTGGGTAGTAGGCTTCTTTGCGTACTTCTTCCCCGGAGCAGAATCAAGGAGGAGAGCCAGCCTAATGCCATGGCACGTTTACTTTGGAATATTCGTCTTCTTAATGGCAACTTGCACTGCTGAATTAGGGCTGCTTCAAAGCTTGGACACTTGGTTTTTGTTGGGGCACACCCAAGAAAACCTTATCATCAACTTCACAGGTCTTCTCATTTTTCTCTATGCAGCCACGGTCGTTCTCACTGTCATTCTTCCACCCCTTTATTAG

Protein sequence

MAFKRNFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVIGLVLFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSLHSWFGLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLLQSLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY
Homology
[Close]
BLAST of Carg21992 vs. NCBI nr
Match: XP_022941965.1 (probable ascorbate-specific transmembrane electron transporter 1 [Cucurbita moschata])

HSP 1 Score: 435.3 bits (1118), Expect = 1.3e-118
Identity = 220/222 (99.10%), Postives = 221/222 (99.55%), Query Frame = 0 

Query: 1   MAFKRNFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVI 60
           MAFKRNFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVI
Sbjct: 1   MAFKRNFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVI 60

Query: 61  GLVLFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSL 120
           GL+LFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSL
Sbjct: 61  GLILFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSL 120

Query: 121 HSWFGLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLL 180
           HSWFGLITVCLYGLQWVVGFF YFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLL
Sbjct: 121 HSWFGLITVCLYGLQWVVGFFTYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLL 180

Query: 181 QSLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 223
           QSLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY
Sbjct: 181 QSLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 222

[Close]
BLAST of Carg21992 vs. NCBI nr
Match: XP_022987904.1 (probable ascorbate-specific transmembrane electron transporter 1 [Cucurbita maxima])

HSP 1 Score: 431.4 bits (1108), Expect = 1.8e-117
Identity = 219/222 (98.65%), Postives = 220/222 (99.10%), Query Frame = 0 

Query: 1   MAFKRNFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVI 60
           MAFKRNFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVI
Sbjct: 1   MAFKRNFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVI 60

Query: 61  GLVLFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSL 120
           GL+LFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFH DIQL NLVSL
Sbjct: 61  GLILFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHRDIQLKNLVSL 120

Query: 121 HSWFGLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLL 180
           HSWFGLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLL
Sbjct: 121 HSWFGLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLL 180

Query: 181 QSLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 223
           QSLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY
Sbjct: 181 QSLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 222

[Close]
BLAST of Carg21992 vs. NCBI nr
Match: XP_022150036.1 (probable ascorbate-specific transmembrane electron transporter 1 [Momordica charantia])

HSP 1 Score: 340.5 bits (872), Expect = 4.3e-90
Identity = 168/223 (75.34%), Postives = 196/223 (87.89%), Query Frame = 0 

Query: 1   MAFKR-NFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMV 60
           MA KR  F I+ARPVT+LGH+VA+++A LIL WILHFREGVA KS NKLKILNLHV+LMV
Sbjct: 1   MASKRGKFQILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMV 60

Query: 61  IGLVLFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVS 120
           +G +LFGG+AIMAYKS+P KKY+RK+VHL LH  ALA  IFG+C++FKFHHD  L++L+S
Sbjct: 61  VGFILFGGEAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLS 120

Query: 121 LHSWFGLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGL 180
           LHSWFGLIT+ LY LQWV GFFAYFFPGAESRRRAS+MPWH+YFGIF+FLMA CTAELGL
Sbjct: 121 LHSWFGLITISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGL 180

Query: 181 LQSLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 223
           LQ  +T  +LGH+QE LI+NFTGLL+ LYAATVVLTVILPPLY
Sbjct: 181 LQRFNT-LILGHSQEGLIVNFTGLLLLLYAATVVLTVILPPLY 222

[Close]
BLAST of Carg21992 vs. NCBI nr
Match: ONI31886.1 (hypothetical protein PRUPE_1G336700 [Prunus persica])

HSP 1 Score: 248.1 bits (632), Expect = 2.9e-62
Identity = 124/218 (56.88%), Postives = 165/218 (75.69%), Query Frame = 0 

Query: 5   RNFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVIGLVL 64
           R++ I A PVT+  HL+AI+I  L+L+W+LHFR G+A  S    KILN+H LLMVIG +L
Sbjct: 6   RSYQISATPVTIFVHLLAIAITTLVLIWLLHFRHGLALNSFITEKILNVHTLLMVIGFIL 65

Query: 65  FGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSLHSWF 124
            GG+AIMAYK++P K+  +K+VHL LH +AL A I GI  +FKF+H+  + NL++LHSW 
Sbjct: 66  IGGEAIMAYKTVPGKRNTQKMVHLILHFLALVAIILGIYAAFKFNHESGIPNLLTLHSWL 125

Query: 125 GLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLLQSLD 184
           G+IT+ L+GLQW+  FFAY FPGAES  R +L+PWH + G+ +FL+A CTAE GLL+   
Sbjct: 126 GIITISLFGLQWLFAFFAYVFPGAESSARGNLVPWHTFVGMVIFLLAVCTAEAGLLERF- 185

Query: 185 TWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 223
            +  +G TQE LIINFTGLLIFL+AA+V L+V+LP LY
Sbjct: 186 LFLSIGRTQETLIINFTGLLIFLFAASVSLSVLLPRLY 222

[Close]
BLAST of Carg21992 vs. NCBI nr
Match: XP_016647009.1 (PREDICTED: probable ascorbate-specific transmembrane electron transporter 1 [Prunus mume])

HSP 1 Score: 247.3 bits (630), Expect = 4.9e-62
Identity = 123/217 (56.68%), Postives = 163/217 (75.12%), Query Frame = 0 

Query: 5   RNFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVIGLVL 64
           R++ + A PVT+  HL+AI+I  L+L+W+LHFR G+A  S    KILN+H LLMVIG +L
Sbjct: 6   RSYQVSATPVTIFAHLLAIAITTLVLIWLLHFRHGLALNSFITEKILNVHTLLMVIGFIL 65

Query: 65  FGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSLHSWF 124
            GG+AIMAYK++P K+  +K+VHL LH +AL A I GI  +FKF+H+  + NL++LHSW 
Sbjct: 66  IGGEAIMAYKTVPGKRNTQKMVHLILHFLALVAIILGIYAAFKFNHESGIPNLLTLHSWL 125

Query: 125 GLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLLQSLD 184
           G+IT+ L+GLQW+  FFAY FPGAES  R +L+PWH + G  +FL+A CTAE GLL+   
Sbjct: 126 GIITISLFGLQWLFAFFAYVFPGAESSARGNLVPWHTFVGTVIFLLAVCTAEAGLLERF- 185

Query: 185 TWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPL 222
            +  +G TQE LIINFTGLLIFL+AA+V LTV+LP L
Sbjct: 186 LFLSIGRTQETLIINFTGLLIFLFAASVSLTVLLPRL 221

[Close]
BLAST of Carg21992 vs. TAIR10
Match: AT4G25570.1 (Cytochrome b561/ferric reductase transmembrane protein family)

HSP 1 Score: 189.1 bits (479), Expect = 2.9e-48
Identity = 94/210 (44.76%), Postives = 140/210 (66.67%), Query Frame = 0 

Query: 9   IMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVIGLVLFGGQ 68
           I A  VT + H +A+  A+++LVW + +R G+A+++ NK  I NLH +LM+IG ++ GG+
Sbjct: 5   INAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFIILGGE 64

Query: 69  AIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSLHSWFGLIT 128
           AI++YKS+P +K  +K++HL LHAIALA  IFGIC +FK H++  + NL SLHSW G+  
Sbjct: 65  AIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWIGIGV 124

Query: 129 VCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLLQS---LDT 188
           + LYG QWV  F  +FFPG  +  ++ L+PWH   G+FV+++A   A LG L+    L+ 
Sbjct: 125 ISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLTFLEN 184

Query: 189 WFLLGHTQENLIINFTGLLIFLYAATVVLT 216
             L  +  E  +INFT ++  L+ A VVLT
Sbjct: 185 GGLDKYGSEAFLINFTAIITILFGAFVVLT 214

[Close]
BLAST of Carg21992 vs. TAIR10
Match: AT5G38630.1 (cytochrome B561-1)

HSP 1 Score: 166.4 bits (420), Expect = 2.0e-41
Identity = 83/211 (39.34%), Postives = 128/211 (60.66%), Query Frame = 0 

Query: 13  PVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVIGLVLFGGQAIMA 72
           P+ ++  ++   IA L+L W +H+R G+A  SDNK  I N+H ++MVIGL+LF G+A++A
Sbjct: 10  PIFMVVRVLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNVHPVMMVIGLILFNGEAMLA 69

Query: 73  YKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSLHSWFGLITVCLY 132
           YKS+   K  +K+VHL L   A   ++ G+  + KFH D  + N  SLHSW GL  + L+
Sbjct: 70  YKSVQGTKNLKKLVHLTLQLTAFILSLIGVWAALKFHIDKGIENFYSLHSWLGLACLFLF 129

Query: 133 GLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLLQSLD----TWFL 192
             QW  GF  Y++PG     RASLMPWHV+ GI ++ +A  TA  G+L+ +        +
Sbjct: 130 AFQWAAGFVTYWYPGGSRNSRASLMPWHVFLGISIYALALVTATTGILEKVTFLQVNQVI 189

Query: 193 LGHTQENLIINFTGLLIFLYAATVVLTVILP 220
             ++ E +++N  G+LI +    V+L V+ P
Sbjct: 190 TRYSTEAMLVNTMGVLILILGGFVILGVVTP 220

[Close]
BLAST of Carg21992 vs. TAIR10
Match: AT1G14730.1 (Cytochrome b561/ferric reductase transmembrane protein family)

HSP 1 Score: 161.8 bits (408), Expect = 4.9e-40
Identity = 89/206 (43.20%), Postives = 122/206 (59.22%), Query Frame = 0 

Query: 15  TLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVIGLVLFGGQAIMAYK 74
           TL+ H   I   VL+L+W+LH+REG+ + SDN LK+LN+H  LM  G +   GQA+M YK
Sbjct: 18  TLVAHFFGILAVVLMLIWLLHYREGIEYGSDNPLKVLNVHPFLMYCGFLFLVGQAMMTYK 77

Query: 75  SIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSLHSWFGLITVCLYGL 134
           +  A    +K+VH GLH I L   I GIC +F+FH  + L ++VSLHSW GL T  L G+
Sbjct: 78  TAYASHQVQKMVHGGLHLIGLVLGIVGICAAFRFHDKVNLKDMVSLHSWIGLTTFILLGV 137

Query: 135 QWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLLQSLDTWFLLGHT-- 194
           QW+ G F +  P + S  R  +MPWHV  G  +  M    A  GL+Q      +LG +  
Sbjct: 138 QWLFGAFTFLAPQSSSGTRTRMMPWHVLGGRALLYMGIVAALTGLMQRAT---MLGQSTN 197

Query: 195 QENLIINFTGLLIFLYAATVVLTVIL 219
            E+ +INF GL I L+  +V  +V L
Sbjct: 198 AESRLINFLGLAILLFGVSVDFSVAL 220

[Close]
BLAST of Carg21992 vs. TAIR10
Match: AT1G26100.1 (Cytochrome b561/ferric reductase transmembrane protein family)

HSP 1 Score: 122.1 bits (305), Expect = 4.3e-28
Identity = 76/212 (35.85%), Postives = 113/212 (53.30%), Query Frame = 0 

Query: 14  VTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVIGLVLFGGQAIMAY 73
           + L   L  + +AV +L W L       F  +  L    LH LLMVIG +L  G+AI+ +
Sbjct: 11  LVLFARLSGLVVAVSVLYWAL-------FLPNLGLSYSTLHPLLMVIGFILVSGEAILIH 70

Query: 74  KSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSLHSWFGLITVCLYG 133
           + +P  +  +K VHL L  +ALA+A+FGI   F +   +   N  SLHSW GL++V L+ 
Sbjct: 71  RWLPGSRKTKKAVHLWLQGMALASAVFGIWTKFHYQRGV-FANFYSLHSWMGLLSVSLFA 130

Query: 134 LQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLLQSLDTWFLL---- 193
            QWV GF +++  G     R + +PWHV+ G++ + +A  TAE GLL+ L   FL     
Sbjct: 131 AQWVTGFMSFWHRGEVRTTRTTFLPWHVFLGLYTYGLAIATAETGLLEKLT--FLQTKRN 190

Query: 194 --GHTQENLIINFTGLLIFLYAATVVLTVILP 220
                 E++ +N  GL + L    V+   ILP
Sbjct: 191 VPRRGSESMTVNGLGLGLALLGCIVITAAILP 212

[Close]
BLAST of Carg21992 vs. Swiss-Prot
Match: sp|A3A9H6|ACET1_ORYSJ (Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0642300 PE=3 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 1.6e-48
Identity = 96/215 (44.65%), Postives = 143/215 (66.51%), Query Frame = 0 

Query: 9   IMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVIGLVLFGGQ 68
           + A P T + H +A++ A ++LVW +HFR G+AF++ NK  I N+H +LM+IG ++ G +
Sbjct: 5   VRAAPFTYVAHALAVAAATMVLVWCIHFRGGLAFEATNKNLIFNVHPVLMLIGYIILGSE 64

Query: 69  AIMAYKSIPAKKY-WRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSLHSWFGLI 128
           AIM YK +P  K+   K++HL LHAIAL     GI  +FKFH++  + NL SLHSW G+ 
Sbjct: 65  AIMVYKVLPTWKHDTTKLIHLILHAIALVFGAVGIYCAFKFHNESGIANLYSLHSWLGIG 124

Query: 129 TVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLLQSLDTWF 188
           T+CLYG+QW+ GF A+FFP A    R  ++PWH+ FG+FV+++A  TAELG L+ L    
Sbjct: 125 TICLYGIQWIFGFVAFFFPRASPSVRKGVLPWHILFGLFVYILALATAELGFLEKLTFLQ 184

Query: 189 LLG---HTQENLIINFTGLLIFLYAATVVLTVILP 220
             G   +  E  ++NFT L++ L+ A+VV+  + P
Sbjct: 185 SSGLDKYGAEAFLVNFTALIVVLFGASVVVAAVSP 219

[Close]
BLAST of Carg21992 vs. Swiss-Prot
Match: sp|Q7XMK3|ACET2_ORYSJ (Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0533500 PE=2 SV=2)

HSP 1 Score: 190.3 bits (482), Expect = 2.3e-47
Identity = 96/215 (44.65%), Postives = 141/215 (65.58%), Query Frame = 0 

Query: 9   IMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVIGLVLFGGQ 68
           + A P T + H +A++ AV++LVW + FR G+AF++DNK  I N+H +LM+IG ++ G +
Sbjct: 7   VKAAPFTYVAHALAVAAAVMVLVWCISFRGGLAFEADNKNLIFNVHPVLMLIGYIILGSE 66

Query: 69  AIMAYKSIPAKKY-WRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSLHSWFGLI 128
           AIM YK  P   +   K++HL LHAIA+     GI  +FKFH++  + NL SLHSW G+ 
Sbjct: 67  AIMIYKIFPKLNHDTTKLIHLILHAIAIVLGAVGIYCAFKFHNESGIANLYSLHSWLGIG 126

Query: 129 TVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLLQSLDTWF 188
           T+ LYG+QW+ GF A+F+PGA    R   +PWHV FG+FV+++   TAELGLL+ L    
Sbjct: 127 TISLYGIQWIFGFVAFFYPGAAPHVRRGALPWHVLFGLFVYVLTLATAELGLLEKLTFLQ 186

Query: 189 LLG---HTQENLIINFTGLLIFLYAATVVLTVILP 220
             G   +  E  ++NFTGL++ L+ A VV+  + P
Sbjct: 187 SSGLDKYGAEAFLVNFTGLVVALFGAAVVVAAVAP 221

[Close]
BLAST of Carg21992 vs. Swiss-Prot
Match: sp|Q8L856|ACFR1_ARATH (Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB561A PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 5.2e-47
Identity = 94/210 (44.76%), Postives = 140/210 (66.67%), Query Frame = 0 

Query: 9   IMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVIGLVLFGGQ 68
           I A  VT + H +A+  A+++LVW + +R G+A+++ NK  I NLH +LM+IG ++ GG+
Sbjct: 5   INAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFIILGGE 64

Query: 69  AIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSLHSWFGLIT 128
           AI++YKS+P +K  +K++HL LHAIALA  IFGIC +FK H++  + NL SLHSW G+  
Sbjct: 65  AIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWIGIGV 124

Query: 129 VCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLLQS---LDT 188
           + LYG QWV  F  +FFPG  +  ++ L+PWH   G+FV+++A   A LG L+    L+ 
Sbjct: 125 ISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLTFLEN 184

Query: 189 WFLLGHTQENLIINFTGLLIFLYAATVVLT 216
             L  +  E  +INFT ++  L+ A VVLT
Sbjct: 185 GGLDKYGSEAFLINFTAIITILFGAFVVLT 214

[Close]
BLAST of Carg21992 vs. Swiss-Prot
Match: sp|C4IYS8|ACFR2_MAIZE (Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 3.7e-45
Identity = 90/215 (41.86%), Postives = 140/215 (65.12%), Query Frame = 0 

Query: 9   IMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVIGLVLFGGQ 68
           + A P T   H +A++ A ++LVW ++FR G+A ++ NK  I N+H +LM+IG ++ GG+
Sbjct: 7   VRAAPFTYAAHALAVAAAAMVLVWAIYFRGGLAIEATNKNLIFNVHPVLMLIGYIIIGGE 66

Query: 69  AIMAYKSIPAKKY-WRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSLHSWFGLI 128
           AIM Y+ +P   +   K++HL LH IAL     GI  +FK H++  + NL SLHSW G+ 
Sbjct: 67  AIMVYRVLPTSNHETNKLIHLVLHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIG 126

Query: 129 TVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLLQS---LD 188
           T+ LYG+QW+VGF  +FFPGA    +  ++PWH+ FG+FV+++A   AELG L+    L+
Sbjct: 127 TITLYGIQWIVGFVTFFFPGAAPNVKKGVLPWHILFGLFVYILALANAELGFLEKLTFLE 186

Query: 189 TWFLLGHTQENLIINFTGLLIFLYAATVVLTVILP 220
           +  L  +  E  ++NFT L++ L+ A+VV+  I P
Sbjct: 187 SSGLDKYGTEAFLVNFTALVVVLFGASVVVAAIAP 221

[Close]
BLAST of Carg21992 vs. Swiss-Prot
Match: sp|Q6I681|ACET1_MAIZE (Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=ZCYB PE=1 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 4.9e-45
Identity = 92/215 (42.79%), Postives = 139/215 (64.65%), Query Frame = 0 

Query: 9   IMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVIGLVLFGGQ 68
           + A P T   H +A++ A ++LVW + FR G+A +S NK  I N+H +LM+IG V+ GG+
Sbjct: 7   VRAAPFTYAAHALAVAAAAMVLVWSIQFRGGLAIESTNKNLIFNVHPVLMLIGYVIIGGE 66

Query: 69  AIMAYKSIPAKKY-WRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSLHSWFGLI 128
           AIM Y+ +P   +   K++HL LH IAL     GI  +FK H++  + NL SLHSW G+ 
Sbjct: 67  AIMVYRVLPTSNHDTTKLIHLILHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIG 126

Query: 129 TVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLLQS---LD 188
           T+ LYG+QW++GF  +FFPGA    +  ++PWHV FG+FV+++A   AELG L+    L+
Sbjct: 127 TITLYGIQWIIGFVTFFFPGAAPNVKKGVLPWHVLFGLFVYILALANAELGFLEKLTFLE 186

Query: 189 TWFLLGHTQENLIINFTGLLIFLYAATVVLTVILP 220
           +  L  +  E  ++NFT L++ L+ A+VV+  I P
Sbjct: 187 SSGLDKYGTEAFLVNFTALVVVLFGASVVVAAIAP 221

[Close]
BLAST of Carg21992 vs. TrEMBL
Match: tr|M5XQI8|M5XQI8_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G336700 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.9e-62
Identity = 124/218 (56.88%), Postives = 165/218 (75.69%), Query Frame = 0 

Query: 5   RNFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVIGLVL 64
           R++ I A PVT+  HL+AI+I  L+L+W+LHFR G+A  S    KILN+H LLMVIG +L
Sbjct: 6   RSYQISATPVTIFVHLLAIAITTLVLIWLLHFRHGLALNSFITEKILNVHTLLMVIGFIL 65

Query: 65  FGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSLHSWF 124
            GG+AIMAYK++P K+  +K+VHL LH +AL A I GI  +FKF+H+  + NL++LHSW 
Sbjct: 66  IGGEAIMAYKTVPGKRNTQKMVHLILHFLALVAIILGIYAAFKFNHESGIPNLLTLHSWL 125

Query: 125 GLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLLQSLD 184
           G+IT+ L+GLQW+  FFAY FPGAES  R +L+PWH + G+ +FL+A CTAE GLL+   
Sbjct: 126 GIITISLFGLQWLFAFFAYVFPGAESSARGNLVPWHTFVGMVIFLLAVCTAEAGLLERF- 185

Query: 185 TWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 223
            +  +G TQE LIINFTGLLIFL+AA+V L+V+LP LY
Sbjct: 186 LFLSIGRTQETLIINFTGLLIFLFAASVSLSVLLPRLY 222

[Close]
BLAST of Carg21992 vs. TrEMBL
Match: tr|A0A0A0KLE5|A0A0A0KLE5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G059150 PE=4 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 6.2e-61
Identity = 114/161 (70.81%), Postives = 139/161 (86.34%), Query Frame = 0 

Query: 62  LVLFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSLH 121
           +VL+G  AIMAYKSIP +KYWRKVVHL LH +ALA  IFG+CM+FKFHH+I+L++L+SLH
Sbjct: 1   MVLWGHAAIMAYKSIPGRKYWRKVVHLVLHLMALACGIFGVCMAFKFHHEIKLSDLLSLH 60

Query: 122 SWFGLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLLQ 181
           SW GLI V LY  QW+ GFF YFFPGAE+RRR +++PWHVYFG+F+FL+ATC AELGLLQ
Sbjct: 61  SWIGLIAVSLYAFQWIFGFFVYFFPGAEARRRGNMLPWHVYFGVFIFLIATCNAELGLLQ 120

Query: 182 SLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 223
             +T  +LGH+Q+ LI+NFTGLL+ LYAATVVLTVILPPLY
Sbjct: 121 RFNT-LVLGHSQQGLIVNFTGLLLLLYAATVVLTVILPPLY 160

[Close]
BLAST of Carg21992 vs. TrEMBL
Match: tr|A0A1U8JQI7|A0A1U8JQI7_GOSHI (probable ascorbate-specific transmembrane electron transporter 1 OS=Gossypium hirsutum OX=3635 GN=LOC107908291 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.8e-60
Identity = 120/217 (55.30%), Postives = 163/217 (75.12%), Query Frame = 0 

Query: 5   RNFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVIGLVL 64
           R++ I A PVT+ GHL+ I++  L+LVW+L FREG+AF+S NK KI NLH LLMVIG +L
Sbjct: 6   RSYQISATPVTVFGHLLFIAVTTLVLVWLLKFREGLAFESANKFKIFNLHPLLMVIGFIL 65

Query: 65  FGGQAIMAYKSIPAKK--YWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSLHS 124
             G+AIMAYKS P+++    +K VHL L  IAL   IFGI + FKFH++  + ++V+LHS
Sbjct: 66  MAGEAIMAYKSTPSRRDIQVQKAVHLTLQTIALGCGIFGIVVIFKFHNETNMPDMVTLHS 125

Query: 125 WFGLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLLQS 184
           W G+IT+CL+GLQ+++GFF+Y FPGAES  RA+ +PWH + G+ +F +A CTAE+GLLQ 
Sbjct: 126 WLGMITICLFGLQYLLGFFSYVFPGAESYSRAAYLPWHTFGGLLIFFLAICTAEMGLLQK 185

Query: 185 LDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILP 220
             + FL   +QE LI+NF GLL+FL+A  V LTV+LP
Sbjct: 186 FLSLFLT-RSQEALIVNFIGLLLFLFAVAVALTVVLP 221

[Close]
BLAST of Carg21992 vs. TrEMBL
Match: tr|A0A067KPM8|A0A067KPM8_JATCU (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_04809 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 4.0e-60
Identity = 115/218 (52.75%), Postives = 158/218 (72.48%), Query Frame = 0 

Query: 5   RNFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVIGLVL 64
           R++ + A P+T++ HL+ +S+A L+LVW+L F +G AFKS N++KILN+H+ LM++G +L
Sbjct: 6   RSYQLSATPITMVAHLIFLSVATLVLVWLLKFEDGFAFKSANEIKILNVHIFLMIVGFIL 65

Query: 65  FGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSLHSWF 124
             G+AIMAYK+IPA++  +K VH  LH IAL A + G+  +FK+ H I   N+V+LHSW 
Sbjct: 66  IAGEAIMAYKTIPAERKVQKAVHFILHLIALVAGVLGVYAAFKYKHQIGDKNMVTLHSWL 125

Query: 125 GLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLLQSLD 184
           G+IT+CL+GLQWV+GFF Y FPGAE   RAS MPWHV+ G+F+F +A CTA+ GL Q   
Sbjct: 126 GMITICLFGLQWVLGFFCYVFPGAEMTARASYMPWHVFGGMFIFFLAICTAQTGLNQRFK 185

Query: 185 TWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 223
           T       QE LI+NFTGLL+ LYA  V L+ +LP  Y
Sbjct: 186 TL-----DQEGLIVNFTGLLLLLYAVGVGLSAVLPRRY 218

[Close]
BLAST of Carg21992 vs. TrEMBL
Match: tr|A0A0D2RFM6|A0A0D2RFM6_GOSRA (Uncharacterized protein OS=Gossypium raimondii OX=29730 GN=B456_005G153100 PE=4 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 5.2e-60
Identity = 120/217 (55.30%), Postives = 163/217 (75.12%), Query Frame = 0 

Query: 5   RNFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMVIGLVL 64
           R++ I A PVT+ GHL+ I++  L+LVW+L FREG+A +S NKLKI NLH LLMVIG +L
Sbjct: 6   RSYQISATPVTVFGHLLFIAVTTLVLVWLLKFREGLASESANKLKIFNLHPLLMVIGFIL 65

Query: 65  FGGQAIMAYKSIPAKK--YWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVSLHS 124
             G+AIMAYKS P+++    +K VHL L  IAL   IFGI + FKFH++  + ++V+LHS
Sbjct: 66  MAGEAIMAYKSTPSRRDIQVQKAVHLTLQTIALGCGIFGIVVIFKFHNETNMPDMVTLHS 125

Query: 125 WFGLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGLLQS 184
           W G+IT+CL+GLQ+++GFF+Y FPGAES  RA+ +PWH + G+ +F +A CTAE+GLLQ 
Sbjct: 126 WLGMITICLFGLQYLLGFFSYVFPGAESYSRAAYLPWHTFGGLLIFFLAICTAEMGLLQK 185

Query: 185 LDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILP 220
             + FL   +QE LI+NF GLL+FL+A  V LTV+LP
Sbjct: 186 FLSLFLT-RSQEALIVNFIGLLLFLFAVAVALTVVLP 221

The following BLAST results are available for this feature:
BLAST of Carg21992 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022941965.11.3e-11899.10probable ascorbate-specific transmembrane electron transporter 1 [Cucurbita mosc... [more]
XP_022987904.11.8e-11798.65probable ascorbate-specific transmembrane electron transporter 1 [Cucurbita maxi... [more]
XP_022150036.14.3e-9075.34probable ascorbate-specific transmembrane electron transporter 1 [Momordica char... [more]
ONI31886.12.9e-6256.88hypothetical protein PRUPE_1G336700 [Prunus persica][more]
XP_016647009.14.9e-6256.68PREDICTED: probable ascorbate-specific transmembrane electron transporter 1 [Pru... [more]
BLAST of Carg21992 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G25570.12.9e-4844.76Cytochrome b561/ferric reductase transmembrane protein family[more]
AT5G38630.12.0e-4139.34cytochrome B561-1[more]
AT1G14730.14.9e-4043.20Cytochrome b561/ferric reductase transmembrane protein family[more]
AT1G26100.14.3e-2835.85Cytochrome b561/ferric reductase transmembrane protein family[more]
BLAST of Carg21992 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|A3A9H6|ACET1_ORYSJ1.6e-4844.65Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa... [more]
sp|Q7XMK3|ACET2_ORYSJ2.3e-4744.65Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa... [more]
sp|Q8L856|ACFR1_ARATH5.2e-4744.76Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB5... [more]
sp|C4IYS8|ACFR2_MAIZE3.7e-4541.86Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2... [more]
sp|Q6I681|ACET1_MAIZE4.9e-4542.79Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=Z... [more]
BLAST of Carg21992 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|M5XQI8|M5XQI8_PRUPE1.9e-6256.88Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G336700 PE=4 SV=1[more]
tr|A0A0A0KLE5|A0A0A0KLE5_CUCSA6.2e-6170.81Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G059150 PE=4 SV=1[more]
tr|A0A1U8JQI7|A0A1U8JQI7_GOSHI1.8e-6055.30probable ascorbate-specific transmembrane electron transporter 1 OS=Gossypium hi... [more]
tr|A0A067KPM8|A0A067KPM8_JATCU4.0e-6052.75Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_04809 PE=4 SV=1[more]
tr|A0A0D2RFM6|A0A0D2RFM6_GOSRA5.2e-6055.30Uncharacterized protein OS=Gossypium raimondii OX=29730 GN=B456_005G153100 PE=4 ... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006593Cyt_b561/ferric_Rdtase_TM
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg21992-RACarg21992-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006593Cytochrome b561/ferric reductase transmembraneSMARTSM00665561_7coord: 53..179
e-value: 2.7E-40
score: 149.9
IPR006593Cytochrome b561/ferric reductase transmembranePFAMPF03188Cytochrom_B561coord: 53..182
e-value: 5.0E-41
score: 140.0
IPR006593Cytochrome b561/ferric reductase transmembranePROSITEPS50939CYTOCHROME_B561coord: 17..217
score: 41.138
NoneNo IPR availableGENE3DG3DSA:1.20.120.1770coord: 7..221
e-value: 1.4E-68
score: 232.5
NoneNo IPR availablePANTHERPTHR10106:SF36SUBFAMILY NOT NAMEDcoord: 4..219
NoneNo IPR availablePANTHERPTHR10106CYTOCHROME B561-RELATEDcoord: 4..219