CSPI05G01400 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI05G01400
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Cytochrome B561, putative
Location	Chr5 : 1920801 .. 1921327 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATTAGCATGTGGAATCTTTGGGGTTTGCATGGCTTTCAAGTTTCACCATGAAATCAAACTTTCAGACCTTCTTTCATTGCATTCTTGGATTGGTTTGATAGCCGTTTCCTTGTATGCTTTCCAGGTCATTTCTCCCCTTAAACATTACTTTACTATAAGATAAAATTAAAGCTAAGTGCTAACGCATCGCAATTATTCAAGATATTTGTACAACATTTTTGTAAGATACATAATAATTGAAGTGTGTATATTCTTATGCAGTGGATATTTGGTTTCTTTGTGTACTTCTTCCCTGGAGCAGAAGCAAGAAGAAGAGGAAACATGTTGCCATGGCATGTTTACTTTGGAGTATTCATATTTTTAATAGCAACATGCAATGCTGAATTAGGGTTACTTCAAAGGTTCAATACATTGGTGTTAGGTCATTCACAACAAGGCCTTATTGTCAACTTCACTGGCCTTCTTCTTCTTCTCTATGCTGCCACTGTTGTTCTCACTGTCATTCTCCCACCACTTTATTAA

mRNA sequence

ATGGCATTAGCATGTGGAATCTTTGGGGTTTGCATGGCTTTCAAGTTTCACCATGAAATCAAACTTTCAGACCTTCTTTCATTGCATTCTTGGATTGGTTTGATAGCCGTTTCCTTGTATGCTTTCCAGTGGATATTTGGTTTCTTTGTGTACTTCTTCCCTGGAGCAGAAGCAAGAAGAAGAGGAAACATGTTGCCATGGCATGTTTACTTTGGAGTATTCATATTTTTAATAGCAACATGCAATGCTGAATTAGGGTTACTTCAAAGGTTCAATACATTGGTGTTAGGTCATTCACAACAAGGCCTTATTGTCAACTTCACTGGCCTTCTTCTTCTTCTCTATGCTGCCACTGTTGTTCTCACTGTCATTCTCCCACCACTTTATTAA

Coding sequence (CDS)

ATGGCATTAGCATGTGGAATCTTTGGGGTTTGCATGGCTTTCAAGTTTCACCATGAAATCAAACTTTCAGACCTTCTTTCATTGCATTCTTGGATTGGTTTGATAGCCGTTTCCTTGTATGCTTTCCAGTGGATATTTGGTTTCTTTGTGTACTTCTTCCCTGGAGCAGAAGCAAGAAGAAGAGGAAACATGTTGCCATGGCATGTTTACTTTGGAGTATTCATATTTTTAATAGCAACATGCAATGCTGAATTAGGGTTACTTCAAAGGTTCAATACATTGGTGTTAGGTCATTCACAACAAGGCCTTATTGTCAACTTCACTGGCCTTCTTCTTCTTCTCTATGCTGCCACTGTTGTTCTCACTGTCATTCTCCCACCACTTTATTAA

Homology

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BLAST of CSPI05G01400 vs. Swiss-Prot
Match: ACFR1_ARATH (Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana GN=CYB561A PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.0e-27
Identity = 56/126 (44.44%), Postives = 83/126 (65.87%), Query Frame = 1


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +ALA GIFG+C AFK H+E  + +L SLHSWIG+  +SLY FQW++ F V+FFPG     
Sbjct: 89  IALALGIFGICAAFKNHNESHIPNLYSLHSWIGIGVISLYGFQWVYSFIVFFFPGGSTNL 148

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLG----HSQQGLIVNFTGLLLLLYA 120
           +  +LPWH   G+F++++A  NA LG L++   L  G    +  +  ++NFT ++ +L+ 
Sbjct: 149 KSGLLPWHAMLGLFVYILAVGNAALGFLEKLTFLENGGLDKYGSEAFLINFTAIITILFG 208

Query: 121 ATVVLT 123
           A VVLT
Sbjct: 209 AFVVLT 214

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BLAST of CSPI05G01400 vs. Swiss-Prot
Match: ACET2_ORYSJ (Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa subsp. japonica GN=Os04g0533500 PE=2 SV=2)

HSP 1 Score: 117.9 bits (294), Expect = 8.5e-26
Identity = 56/130 (43.08%), Postives = 83/130 (63.85%), Query Frame = 1


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +A+  G  G+  AFKFH+E  +++L SLHSW+G+  +SLY  QWIFGF  +F+PGA    
Sbjct: 92  IAIVLGAVGIYCAFKFHNESGIANLYSLHSWLGIGTISLYGIQWIFGFVAFFYPGAAPHV 151

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLG----HSQQGLIVNFTGLLLLLYA 120
           R   LPWHV FG+F++++    AELGLL++   L       +  +  +VNFTGL++ L+ 
Sbjct: 152 RRGALPWHVLFGLFVYVLTLATAELGLLEKLTFLQSSGLDKYGAEAFLVNFTGLVVALFG 211

Query: 121 ATVVLTVILP 127
           A VV+  + P
Sbjct: 212 AAVVVAAVAP 221

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BLAST of CSPI05G01400 vs. Swiss-Prot
Match: ACET1_MAIZE (Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays GN=ZCYB PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 4.2e-25
Identity = 56/130 (43.08%), Postives = 84/130 (64.62%), Query Frame = 1


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +AL  G  G+  AFK H+E  +++L SLHSWIG+  ++LY  QWI GF  +FFPGA    
Sbjct: 92  IALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTITLYGIQWIIGFVTFFFPGAAPNV 151

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLG----HSQQGLIVNFTGLLLLLYA 120
           +  +LPWHV FG+F++++A  NAELG L++   L       +  +  +VNFT L+++L+ 
Sbjct: 152 KKGVLPWHVLFGLFVYILALANAELGFLEKLTFLESSGLDKYGTEAFLVNFTALVVVLFG 211

Query: 121 ATVVLTVILP 127
           A+VV+  I P
Sbjct: 212 ASVVVAAIAP 221

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BLAST of CSPI05G01400 vs. Swiss-Prot
Match: ACFR2_MAIZE (Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 7.2e-25
Identity = 55/130 (42.31%), Postives = 84/130 (64.62%), Query Frame = 1


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +AL  G  G+  AFK H+E  +++L SLHSWIG+  ++LY  QWI GF  +FFPGA    
Sbjct: 92  IALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTITLYGIQWIVGFVTFFFPGAAPNV 151

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLG----HSQQGLIVNFTGLLLLLYA 120
           +  +LPWH+ FG+F++++A  NAELG L++   L       +  +  +VNFT L+++L+ 
Sbjct: 152 KKGVLPWHILFGLFVYILALANAELGFLEKLTFLESSGLDKYGTEAFLVNFTALVVVLFG 211

Query: 121 ATVVLTVILP 127
           A+VV+  I P
Sbjct: 212 ASVVVAAIAP 221

[Close]

BLAST of CSPI05G01400 vs. Swiss-Prot
Match: ACET1_ORYSJ (Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa subsp. japonica GN=Os02g0642300 PE=3 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 1.2e-24
Identity = 54/130 (41.54%), Postives = 83/130 (63.85%), Query Frame = 1


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +AL  G  G+  AFKFH+E  +++L SLHSW+G+  + LY  QWIFGF  +FFP A    
Sbjct: 90  IALVFGAVGIYCAFKFHNESGIANLYSLHSWLGIGTICLYGIQWIFGFVAFFFPRASPSV 149

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLG----HSQQGLIVNFTGLLLLLYA 120
           R  +LPWH+ FG+F++++A   AELG L++   L       +  +  +VNFT L+++L+ 
Sbjct: 150 RKGVLPWHILFGLFVYILALATAELGFLEKLTFLQSSGLDKYGAEAFLVNFTALIVVLFG 209

Query: 121 ATVVLTVILP 127
           A+VV+  + P
Sbjct: 210 ASVVVAAVSP 219

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BLAST of CSPI05G01400 vs. TrEMBL
Match: A0A0A0KLE5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G059150 PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 2.6e-66
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 1


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR
Sbjct: 32  MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 91

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 120
           RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV
Sbjct: 92  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 151

Query: 121 LTVILPPLY 130
           LTVILPPLY
Sbjct: 152 LTVILPPLY 160

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BLAST of CSPI05G01400 vs. TrEMBL
Match: M5XQI8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa026406mg PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 2.7e-34
Identity = 71/129 (55.04%), Postives = 98/129 (75.97%), Query Frame = 1


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +AL   I G+  AFKF+HE  + +LL+LHSW+G+I +SL+  QW+F FF Y FPGAE+  
Sbjct: 94  LALVAIILGIYAAFKFNHESGIPNLLTLHSWLGIITISLFGLQWLFAFFAYVFPGAESSA 153

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 120
           RGN++PWH + G+ IFL+A C AE GLL+RF  L +G +Q+ LI+NFTGLL+ L+AA+V 
Sbjct: 154 RGNLVPWHTFVGMVIFLLAVCTAEAGLLERFLFLSIGRTQETLIINFTGLLIFLFAASVS 213

Query: 121 LTVILPPLY 130
           L+V+LP LY
Sbjct: 214 LSVLLPRLY 222

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BLAST of CSPI05G01400 vs. TrEMBL
Match: A0A0D2RFM6_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G153100 PE=4 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 1.5e-32
Identity = 68/126 (53.97%), Postives = 92/126 (73.02%), Query Frame = 1


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +AL CGIFG+ + FKFH+E  + D+++LHSW+G+I + L+  Q++ GFF Y FPGAE+  
Sbjct: 96  IALGCGIFGIVVIFKFHNETNMPDMVTLHSWLGMITICLFGLQYLLGFFSYVFPGAESYS 155

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 120
           R   LPWH + G+ IF +A C AE+GLLQ+F +L L  SQ+ LIVNF GLLL L+A  V 
Sbjct: 156 RAAYLPWHTFGGLLIFFLAICTAEMGLLQKFLSLFLTRSQEALIVNFIGLLLFLFAVAVA 215

Query: 121 LTVILP 127
           LTV+LP
Sbjct: 216 LTVVLP 221

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BLAST of CSPI05G01400 vs. TrEMBL
Match: V4U5Y4_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10017511mg PE=4 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.1e-31
Identity = 67/125 (53.60%), Postives = 87/125 (69.60%), Query Frame = 1


Query: 2   ALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARRR 61
           AL  G+ G+   FKFHHE  L ++ +LHSW+G+I + LY  QW+  FF Y FPGAE   R
Sbjct: 76  ALLAGVIGIYAVFKFHHESGLPNMFTLHSWLGMITICLYGLQWLLAFFSYVFPGAEMSAR 135

Query: 62  GNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVVL 121
           G+ LPWH +FG+ IF +A C AE GL+Q+F  L L   Q+ LIVNFTGLL+LL+  +V L
Sbjct: 136 GSFLPWHSFFGLVIFFLAICTAETGLVQKFTFLGLFRDQEALIVNFTGLLILLFGISVGL 195

Query: 122 TVILP 127
           TV+LP
Sbjct: 196 TVVLP 200

[Close]

BLAST of CSPI05G01400 vs. TrEMBL
Match: A0A067KPM8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04809 PE=4 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 4.0e-30
Identity = 68/129 (52.71%), Postives = 90/129 (69.77%), Query Frame = 1


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +AL  G+ GV  AFK+ H+I   ++++LHSW+G+I + L+  QW+ GFF Y FPGAE   
Sbjct: 94  IALVAGVLGVYAAFKYKHQIGDKNMVTLHSWLGMITICLFGLQWVLGFFCYVFPGAEMTA 153

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 120
           R + +PWHV+ G+FIF +A C A+ GL QRF TL     Q+GLIVNFTGLLLLLYA  V 
Sbjct: 154 RASYMPWHVFGGMFIFFLAICTAQTGLNQRFKTL----DQEGLIVNFTGLLLLLYAVGVG 213

Query: 121 LTVILPPLY 130
           L+ +LP  Y
Sbjct: 214 LSAVLPRRY 218

[Close]

BLAST of CSPI05G01400 vs. TAIR10
Match: AT4G25570.1 (AT4G25570.1 Cytochrome b561/ferric reductase transmembrane protein family)

HSP 1 Score: 123.2 bits (308), Expect = 1.1e-28
Identity = 56/126 (44.44%), Postives = 83/126 (65.87%), Query Frame = 1


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +ALA GIFG+C AFK H+E  + +L SLHSWIG+  +SLY FQW++ F V+FFPG     
Sbjct: 89  IALALGIFGICAAFKNHNESHIPNLYSLHSWIGIGVISLYGFQWVYSFIVFFFPGGSTNL 148

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLG----HSQQGLIVNFTGLLLLLYA 120
           +  +LPWH   G+F++++A  NA LG L++   L  G    +  +  ++NFT ++ +L+ 
Sbjct: 149 KSGLLPWHAMLGLFVYILAVGNAALGFLEKLTFLENGGLDKYGSEAFLINFTAIITILFG 208

Query: 121 ATVVLT 123
           A VVLT
Sbjct: 209 AFVVLT 214

[Close]

BLAST of CSPI05G01400 vs. TAIR10
Match: AT5G38630.1 (AT5G38630.1 cytochrome B561-1)

HSP 1 Score: 96.7 bits (239), Expect = 1.1e-20
Identity = 46/130 (35.38%), Postives = 76/130 (58.46%), Query Frame = 1


Query: 2   ALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARRR 61
           A    + GV  A KFH +  + +  SLHSW+GL  + L+AFQW  GF  Y++PG     R
Sbjct: 91  AFILSLIGVWAALKFHIDKGIENFYSLHSWLGLACLFLFAFQWAAGFVTYWYPGGSRNSR 150

Query: 62  GNMLPWHVYFGVFIFLIATCNAELGLLQR-----FNTLVLGHSQQGLIVNFTGLLLLLYA 121
            +++PWHV+ G+ I+ +A   A  G+L++      N ++  +S + ++VN  G+L+L+  
Sbjct: 151 ASLMPWHVFLGISIYALALVTATTGILEKVTFLQVNQVITRYSTEAMLVNTMGVLILILG 210

Query: 122 ATVVLTVILP 127
             V+L V+ P
Sbjct: 211 GFVILGVVTP 220

[Close]

BLAST of CSPI05G01400 vs. TAIR10
Match: AT1G14730.1 (AT1G14730.1 Cytochrome b561/ferric reductase transmembrane protein family)

HSP 1 Score: 94.7 bits (234), Expect = 4.3e-20
Identity = 48/125 (38.40%), Postives = 71/125 (56.80%), Query Frame = 1


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           + L  GI G+C AF+FH ++ L D++SLHSWIGL    L   QW+FG F +  P + +  
Sbjct: 96  IGLVLGIVGICAAFRFHDKVNLKDMVSLHSWIGLTTFILLGVQWLFGAFTFLAPQSSSGT 155

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 120
           R  M+PWHV  G  +  +    A  GL+QR   L    + +  ++NF GL +LL+  +V 
Sbjct: 156 RTRMMPWHVLGGRALLYMGIVAALTGLMQRATMLGQSTNAESRLINFLGLAILLFGVSVD 215

Query: 121 LTVIL 126
            +V L
Sbjct: 216 FSVAL 220

[Close]

BLAST of CSPI05G01400 vs. TAIR10
Match: AT1G26100.1 (AT1G26100.1 Cytochrome b561/ferric reductase transmembrane protein family)

HSP 1 Score: 83.2 bits (204), Expect = 1.3e-16
Identity = 48/131 (36.64%), Postives = 71/131 (54.20%), Query Frame = 1


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           MALA  +FG+   F +   +  ++  SLHSW+GL++VSL+A QW+ GF  ++  G     
Sbjct: 83  MALASAVFGIWTKFHYQRGV-FANFYSLHSWMGLLSVSLFAAQWVTGFMSFWHRGEVRTT 142

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTL-----VLGHSQQGLIVNFTGLLLLLY 120
           R   LPWHV+ G++ + +A   AE GLL++   L     V     + + VN  GL L L 
Sbjct: 143 RTTFLPWHVFLGLYTYGLAIATAETGLLEKLTFLQTKRNVPRRGSESMTVNGLGLGLALL 202

Query: 121 AATVVLTVILP 127
              V+   ILP
Sbjct: 203 GCIVITAAILP 212

[Close]

BLAST of CSPI05G01400 vs. NCBI nr
Match: gi|778708170|ref|XP_011656135.1| (PREDICTED: transmembrane ascorbate ferrireductase 1-like [Cucumis sativus])

HSP 1 Score: 259.2 bits (661), Expect = 3.8e-66
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 1


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR
Sbjct: 23  MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 82

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 120
           RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV
Sbjct: 83  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 142

Query: 121 LTVILPPLY 130
           LTVILPPLY
Sbjct: 143 LTVILPPLY 151

[Close]

BLAST of CSPI05G01400 vs. NCBI nr
Match: gi|700194502|gb|KGN49679.1| (hypothetical protein Csa_5G059150 [Cucumis sativus])

HSP 1 Score: 259.2 bits (661), Expect = 3.8e-66
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 1


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR
Sbjct: 32  MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 91

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 120
           RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV
Sbjct: 92  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 151

Query: 121 LTVILPPLY 130
           LTVILPPLY
Sbjct: 152 LTVILPPLY 160

[Close]

BLAST of CSPI05G01400 vs. NCBI nr
Match: gi|659109749|ref|XP_008454856.1| (PREDICTED: transmembrane ascorbate ferrireductase 1-like [Cucumis melo])

HSP 1 Score: 253.4 bits (646), Expect = 2.1e-64
Identity = 125/129 (96.90%), Postives = 128/129 (99.22%), Query Frame = 1


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           MALA GIFGVCMAFKFHHEIKLSDLLSLHSWIGLIA+SLYAFQWIFGFFVYFFPGAE+RR
Sbjct: 23  MALASGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAISLYAFQWIFGFFVYFFPGAESRR 82

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 120
           RGNMLPWHVYFG+FIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV
Sbjct: 83  RGNMLPWHVYFGIFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 142

Query: 121 LTVILPPLY 130
           LTVILPPLY
Sbjct: 143 LTVILPPLY 151

[Close]

BLAST of CSPI05G01400 vs. NCBI nr
Match: gi|1009154749|ref|XP_015895343.1| (PREDICTED: probable ascorbate-specific transmembrane electron transporter 1 [Ziziphus jujuba])

HSP 1 Score: 158.7 bits (400), Expect = 7.0e-36
Identity = 74/126 (58.73%), Postives = 97/126 (76.98%), Query Frame = 1


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +AL  GI G+   FK+ HE  LSD+++LHSW+G+I +SLY  Q++ GFF YFFPGAE   
Sbjct: 94  IALLAGILGIYAVFKYKHEAGLSDMVTLHSWLGIITISLYGLQYLLGFFTYFFPGAEMST 153

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 120
           RGN LPWHV+ G+ IF++A C AE GLL+RF  L LG +Q+GLIVNFTGLLL+L+A +V 
Sbjct: 154 RGNFLPWHVFVGILIFVLAICTAETGLLRRFLFLGLGKNQEGLIVNFTGLLLVLFAVSVT 213

Query: 121 LTVILP 127
           LT++LP
Sbjct: 214 LTILLP 219

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BLAST of CSPI05G01400 vs. NCBI nr
Match: gi|596296292|ref|XP_007227148.1| (hypothetical protein PRUPE_ppa026406mg [Prunus persica])

HSP 1 Score: 152.9 bits (385), Expect = 3.8e-34
Identity = 71/129 (55.04%), Postives = 98/129 (75.97%), Query Frame = 1


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +AL   I G+  AFKF+HE  + +LL+LHSW+G+I +SL+  QW+F FF Y FPGAE+  
Sbjct: 94  LALVAIILGIYAAFKFNHESGIPNLLTLHSWLGIITISLFGLQWLFAFFAYVFPGAESSA 153

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 120
           RGN++PWH + G+ IFL+A C AE GLL+RF  L +G +Q+ LI+NFTGLL+ L+AA+V 
Sbjct: 154 RGNLVPWHTFVGMVIFLLAVCTAEAGLLERFLFLSIGRTQETLIINFTGLLIFLFAASVS 213

Query: 121 LTVILPPLY 130
           L+V+LP LY
Sbjct: 214 LSVLLPRLY 222

The following BLAST results are available for this feature:

BLAST of CSPI05G01400 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ACFR1_ARATH	2.0e-27	44.44	Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana GN=CYB561A PE=1...	[more]
ACET2_ORYSJ	8.5e-26	43.08	Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa...	[more]
ACET1_MAIZE	4.2e-25	43.08	Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays GN=ZCYB PE=1...	[more]
ACFR2_MAIZE	7.2e-25	42.31	Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays PE=2 SV=1	[more]
ACET1_ORYSJ	1.2e-24	41.54	Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa...	[more]

BLAST of CSPI05G01400 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KLE5_CUCSA	2.6e-66	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G059150 PE=4 SV=1	[more]
M5XQI8_PRUPE	2.7e-34	55.04	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa026406mg PE=4 SV=1	[more]
A0A0D2RFM6_GOSRA	1.5e-32	53.97	Uncharacterized protein OS=Gossypium raimondii GN=B456_005G153100 PE=4 SV=1	[more]
V4U5Y4_9ROSI	2.1e-31	53.60	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10017511mg PE=4 SV=1	[more]
A0A067KPM8_JATCU	4.0e-30	52.71	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04809 PE=4 SV=1	[more]

BLAST of CSPI05G01400 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G25570.1	1.1e-28	44.44	Cytochrome b561/ferric reductase transmembrane protein family	[more]
AT5G38630.1	1.1e-20	35.38	cytochrome B561-1	[more]
AT1G14730.1	4.3e-20	38.40	Cytochrome b561/ferric reductase transmembrane protein family	[more]
AT1G26100.1	1.3e-16	36.64	Cytochrome b561/ferric reductase transmembrane protein family	[more]

BLAST of CSPI05G01400 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778708170\|ref\|XP_011656135.1\|	3.8e-66	100.00	PREDICTED: transmembrane ascorbate ferrireductase 1-like [Cucumis sativus]	[more]
gi\|700194502\|gb\|KGN49679.1\|	3.8e-66	100.00	hypothetical protein Csa_5G059150 [Cucumis sativus]	[more]
gi\|659109749\|ref\|XP_008454856.1\|	2.1e-64	96.90	PREDICTED: transmembrane ascorbate ferrireductase 1-like [Cucumis melo]	[more]
gi\|1009154749\|ref\|XP_015895343.1\|	7.0e-36	58.73	PREDICTED: probable ascorbate-specific transmembrane electron transporter 1 [Ziz...	[more]
gi\|596296292\|ref\|XP_007227148.1\|	3.8e-34	55.04	hypothetical protein PRUPE_ppa026406mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006593	Cyt_b561/ferric_Rdtase_TM

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0008150	biological_process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G01400.1	CSPI05G01400.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006593	Cytochrome b561/ferric reductase transmembrane	PFAM	PF03188	Cytochrom_B561	coord: 1..90 score: 3.0
IPR006593	Cytochrome b561/ferric reductase transmembrane	SMART	SM00665	561_7	coord: 1..87 score: 1.
IPR006593	Cytochrome b561/ferric reductase transmembrane	PROFILE	PS50939	CYTOCHROME_B561	coord: 1..124 score: 29
None	No IPR available	PANTHER	PTHR10106	CYTOCHROME B561-RELATED	coord: 1..126 score: 4.6
None	No IPR available	PANTHER	PTHR10106:SF19	SUBFAMILY NOT NAMED	coord: 1..126 score: 4.6

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI05G01400	Cla007503	Watermelon (97103) v1	cpiwmB397
CSPI05G01400	Csa5G059150	Cucumber (Chinese Long) v2	cpicuB240
CSPI05G01400	ClCG07G006790	Watermelon (Charleston Gray)	cpiwcgB425
CSPI05G01400	Lsi04G011700	Bottle gourd (USVL1VR-Ls)	cpilsiB374
CSPI05G01400	CsaV3_5G015390	Cucumber (Chinese Long) v3	cpicucB286
CSPI05G01400	Cla97C07G134490	Watermelon (97103) v2	cpiwmbB418
CSPI05G01400	Bhi07G000232	Wax gourd	cpiwgoB512
CSPI05G01400	Cucsa.261720	Cucumber (Gy14) v1	cgycpiB398
CSPI05G01400	CmaCh04G000380	Cucurbita maxima (Rimu)	cmacpiB752
CSPI05G01400	CmoCh04G000360	Cucurbita moschata (Rifu)	cmocpiB744
CSPI05G01400	Cp4.1LG01g06320	Cucurbita pepo (Zucchini)	cpecpiB434
CSPI05G01400	CsGy5G011590	Cucumber (Gy14) v2	cgybcpiB227
CSPI05G01400	Carg21992	Silver-seed gourd	carcpiB0206

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI05G01400	Cucurbita maxima (Rimu)	cmacpiB750
CSPI05G01400	Cucurbita maxima (Rimu)	cmacpiB767
CSPI05G01400	Cucurbita moschata (Rifu)	cmocpiB735
CSPI05G01400	Cucurbita moschata (Rifu)	cmocpiB754
CSPI05G01400	Melon (DHL92) v3.5.1	cpimeB377
CSPI05G01400	Cucurbita pepo (Zucchini)	cpecpiB433
CSPI05G01400	Bottle gourd (USVL1VR-Ls)	cpilsiB340
CSPI05G01400	Melon (DHL92) v3.6.1	cpimedB368
CSPI05G01400	Melon (DHL92) v3.6.1	cpimedB369
CSPI05G01400	Silver-seed gourd	carcpiB0705
CSPI05G01400	Watermelon (97103) v2	cpiwmbB383