Carg21159 (gene) Silver-seed gourd

NameCarg21159
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionRibosomal RNA large subunit methyltransferase H
LocationCucurbita_argyrosperma_scaffold_207 : 324685 .. 328859 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TAATTTTAATCAAATTTGTTCTCTCTTTTTCTCACGGTGGCAACTTGCTAACTGACACGTCATCATCTCCGTCTTAGCCCGAGGGGTCTGGTTTCTGCTATCGGCTTCTTATCAATGGTTACTGCTCTCTGTGCTACCATTTCCGCTTCTCCTTCAGGTATCATCGTTTTCTCTACAATAGAAGGAAAAAGAAACCCTAGAATTTGACCTAGGTTTTGTTCGTCTATGTTCTTACCGGCGTTGTGTAATGTTTCTCCGATTTCGAGCTTTCAGTTGGACGTTGCAAGTACACCGGTCAATCTGTGGTACATTTCTTGTTTCTTTAAATTTAGTTTCGTTGTTTGGTTCTTGTGATTTTTGTCTTCGTTGTGATTCTTTTTCCTTTTTACGATCTCGATCGTTCGATTGACGTTTCGTTGTGTTTTGCAATTCCATTTATTAATATCTGATATATTTGGCTGAGATTTCAGTGTGGTTGAGGCTCAGGATGACTGAGTTTTAACTGAGATTAGTCTTTTATGGACAATGAACAGAGAGCGGTGCCGATACGCGTTTTAACTGTGGGGAAGAAGAGGTCTCGTGGCGTTCAGCTTCTCATTGATGAGTATATTGACAAGCTCAATAACTACTGTAATGTGGAAGATGTTCAACTGCGATCAAATCCTAGAAATGCGCGGTATGTGATTGTACGGTTGGTTTTCATTTATCAGTTTTCCTATTTACGCTCGAACTTCCAATCTCTGATGAATGATGCTAATCTTAATGAATGCTTTTCATTCCATGTTTCGTTTCTGCATTATAGGGATCCAAGAGCTCAGGTTGATGATGAGGACATAGCTGTTGTGAATCTTCTCAAGTCCGACGATTGGGTTTGTCTCTTAACATTCATTTTCGTTGAATCATTACGAGTTTTGTTATTCTGGCTGTGCTCTTTTATTTGGTAACTAGTTTAGAACTCTCTTTGTGAGACCTCACGTCGATTAAAGAGGGGAACAAATGCCAGAGAGGACACTGGACTCCAAAGGGGGTGGATTGTGAAATCCCACGCTGGTTGGAGAGGGGAACGAAGCATTCTTTATAAGGGTGTGGAAACCTCTCTCTAGTTTAGAACTAGTTTAGAGCTGTCCCTGTGAGATCCCACATCGATTGAAAACGGGGAACGAATGCCAACGAGGACGCTGGACCCCAAAAGGGGTGGATTGTGAGATCCCACATCGGTTGGAGAGGGAAACAAAGCATTCTTTGTAAGGGCGTGGAAACTTCTCTCTAGCATATGCATTTTAAAACCTTGAGGGAAGCCTGAAAGGGAAAGCCCAAAGAAGACAATATCTACTAGTGGTGGACTTGGACGGTTACAAATCTCTCTCTCCGTCCCTCAATTACATCAGTCTTTTGCCAGCTAAAATGTGAAAGAAAATTGATCAGAGTGCTTCTTTTTCAGAAAAGAAATATAGTTTGCTATGTGCTTTGATTTTATTTTTAGCCCTTCTTCTAAAACTGATCTTTGAAATAGTTTATAATGGTCGTGCTTACTGCTTACAGTGTGTACCATATCCATTCTGCATATGCACCTTCAGGTTGTGATGTTGGATGAGAATGGGAAAGAGATATCCTCCGAGCAGATGGCTGAGTTGGTGGCAGATGCAGGCAATACAGTGAGTACCATTTCCAATTCAAATCAAATGGGTTGCATTGTTATCATCAATTCATCTCTACTGGACTGATTCCTAATAGTTCAAAGTTCAAAGTTAGTGGCTAAGGCCCCATTTGAGAACGAATTGGTTTGGTTTTTTGTTTTCGAAAATTAAGCTTATAAATACTACTTCCACCTATGGATTTCCTTGTTTTGTTATCCACTTGTTACGTCTATTTTAAAAAATCGAGCCAAAGTTTTGAAAAACTAAGAAAACTAGTTTTCAAAAACTTGTTTTTGTCTTTGGATCTTGGCTAAGAATTTGTTTTCTCAAGAGAAATGATACGAAAATAAACACAATTTTCAAAAACAAAATAGTTATCTAACAGGAGTTTTTGCTTGAGGCACCTCTAAGTTAAACCAGCCCACATGTTCAACCTCCATGTCAGTCTAGATCTTAATATGTAACAGTCTCCTGCTTTCTAATTTGATGTTAAGAAAGTTTTGTATTAATCACCATGGTTTAATTGTTTCTGTAATCAAATTTGTTAGAACTATGGGTGTATCCCAGAATGAAAATGGCATTTCTTATGTTTCTTCTTCTTTGCAGGGTGCTTCAAGATTAGCATTTTGCATTGGTGGACCTTATGGCCATGGACAACAACTGCGACAACGAGCTAACGTATCGATCAAGCTGTCCTCCATGGTCTTAAATCATCAAATTGCATTACTTGTGCTTATGGAACAACTTTACAGGTACTTTTTTTGTTTCTTCTCTTCTAATCATTAGTGATTTCGAGTTTCATTTGCAACATCAAATGCTCCTCAAGCAGAGCTTTCCCAAAATGATGTGTTGGGTGGAGTATCCACTTACATTAATGTGATCTAGTACAATAAAGTAACAAGTACCACATAGTGAATATATAGAAATCCAAATCCAAATCCACCGAATTACTCCGCTTATGATCTTCTACATTCTAGGTCTTCCCGTTGCTTCATCTTATTTATGTTCTTCCTATAGCATTTAGACCGAATGACTATGAAATTACGTCGATACTTCTTCATATTACGAATATCGTAGGAAACATCTCTATTTTTTTCTCTTGAGAATCCTTAGAATTACGACTTCGATAATAATGAACTCAAGAGGTTAGACGTATAAAATACTGTGGGACTTTCAGTTTTGCTCAAATCAGCCAAGAATCATGATGCTCCTCTTTCTCTCTCTATCTCCATCTAGAGATTTATTCAAACCTCAATTCAACCCTGAAAAACAGACAAAACTAACAATGCACCATCTTTGCATCTGCGAAGCTTTTTGTATTATATAATGCTTATTCTGAATGTATGTATTCTTTTAGGTCATGGACTATTCTGAAGGGTCAGAAGTACCATCATTAGTAGCAGTGTTCGTACGGTATTTTCATATCTTTTTTTATTTATTGGAAGAGACCTTTTAATGGTAGTTTATTATTGTGTCGGGATGAATATTTAGAAGTTTAAATATCATTTTGGTCCATGTACTTTTAGTCTCATTTCATTATTATAGTCTCTTACAAACAAACTCGTACTTCTTGATAGTTTGTCGTCGGGACGGGGCAGGGGCTTCCTCTGCCATCACCATCAGACCTATCTAGTGAGTTCTTGTGGAGTGGGTCGGATACCTAATTTGGCTGTAGCACATCAGCTAAATATAGAGGGTGAGGGGAGTTTCATCCCGAGGCTATCCCGAGGCTGCCCCTTCAAGCAGTATTCACCTTAAAAAAAAAAAAATTAAGAAAATTTGTTATAGGTGACAAAATTAATTGTTAGTTTTGTGTTTTTAAATATTATGTTTATTTCTTCATATTTTTTCAATATATTTTTCACCTTTTCATAAAAACATATTTCATTTCATGGCAAAATTTCAAAATCACGAACAATTTATTATTACTACTACTTTATTTTCAAAAACAAATTGTTTAAGATTTTGAAAATATTTATAAAATGTAGTGGATAAATGTTTAAACTTAATTTTTAAACCTAAAAAACAAAAATAAAATAGTTAGCAAACGTCAGGCGTAAGTTGTATGGGTTTATAAATATAATTTTATATGTTATTTGTGAATTTTAAAAAAAAATTCACAATGTATGAAAAATAATTTAACATATTAAATTAGAGGTCGGCTTGAATTATCGGACCGCTAGAACGGTGTTCGTATCAACGGTCATGTTGAAAATTACGCGCGTTTTATTAAACGGGAAAATTGTAAGGTGGCGGCAAACAAGTCATCACGTGGACCTAATTCAAACGTATCAACGCCACGTCACAGCCAACTATAATACACGTGTCATCATCTCAGCTAATTAAATCTTCACAGGGCAACTTAAGACCATATCAGCATTTCAACAATGGAATCCCATTCTCGTCTCTTCAATTGTTGAGAACACTCCCTACTTCTTCCTCTCTGAAACCTGCGGTCCAAATTGCCTCAAAATCTGCTCGCCATCAATTTTTCCGACGAATCTCGACCATGAATTCGAGATCGAAGCTAGATTCTAG

mRNA sequence

TAATTTTAATCAAATTTGTTCTCTCTTTTTCTCACGGTGGCAACTTGCTAACTGACACGTCATCATCTCCGTCTTAGCCCGAGGGGTCTGGTTTCTGCTATCGGCTTCTTATCAATGGTTACTGCTCTCTGTGCTACCATTTCCGCTTCTCCTTCAGGTATCATCGTTTTCTCTACAATAGAAGGAAAAAGAAACCCTAGAATTTGACCTAGGTTTTGTTCGTCTATGTTCTTACCGGCGTTGTGTAATGTTTCTCCGATTTCGAGCTTTCAGTTGGACGTTGCAAGTACACCGGTCAATCTGTGGTACATTTCTTGTTTCTTTAAATTTAGTTTCGTTGTTTGGTTCTTGTGATTTTTGTCTTCGTTGTGATTCTTTTTCCTTTTTACGATCTCGATCGTTCGATTGACGTTTCGTTGTGTTTTGCAATTCCATTTATTAATATCTGATATATTTGGCTGAGATTTCAGTGTGGTTGAGGCTCAGGATGACTGAGTTTTAACTGAGATTAGTCTTTTATGGACAATGAACAGAGAGCGGTGCCGATACGCGTTTTAACTGTGGGGAAGAAGAGGTCTCGTGGCGTTCAGCTTCTCATTGATGAGTATATTGACAAGCTCAATAACTACTGTAATGTGGAAGATGTTCAACTGCGATCAAATCCTAGAAATGCGCGGGATCCAAGAGCTCAGGTTGATGATGAGGACATAGCTGTTGTGAATCTTCTCAAGTCCGACGATTGGGTTGTGATGTTGGATGAGAATGGGAAAGAGATATCCTCCGAGCAGATGGCTGAGTTGGTGGCAGATGCAGGCAATACAGGTGCTTCAAGATTAGCATTTTGCATTGGTGGACCTTATGGCCATGGACAACAACTGCGACAACGAGCTAACGTATCGATCAAGCTGTCCTCCATGGTCTTAAATCATCAAATTGCATTACTTGTGCTTATGGAACAACTTTACAGGGGCTTCCTCTGCCATCACCATCAGACCTATCTAGTGAGTTCTTGTGGAGTGGGTCGGATACCTAATTTGGCTGTAGCACATCAGCTAAATATAGAGGGGCAACTTAAGACCATATCAGCATTTCAACAATGGAATCCCATTCTCGTCTCTTCAATTGTTGAGAACACTCCCTACTTCTTCCTCTCTGAAACCTGCGGTCCAAATTGCCTCAAAATCTGCTCGCCATCAATTTTTCCGACGAATCTCGACCATGAATTCGAGATCGAAGCTAGATTCTAG

Coding sequence (CDS)

ATGGACAATGAACAGAGAGCGGTGCCGATACGCGTTTTAACTGTGGGGAAGAAGAGGTCTCGTGGCGTTCAGCTTCTCATTGATGAGTATATTGACAAGCTCAATAACTACTGTAATGTGGAAGATGTTCAACTGCGATCAAATCCTAGAAATGCGCGGGATCCAAGAGCTCAGGTTGATGATGAGGACATAGCTGTTGTGAATCTTCTCAAGTCCGACGATTGGGTTGTGATGTTGGATGAGAATGGGAAAGAGATATCCTCCGAGCAGATGGCTGAGTTGGTGGCAGATGCAGGCAATACAGGTGCTTCAAGATTAGCATTTTGCATTGGTGGACCTTATGGCCATGGACAACAACTGCGACAACGAGCTAACGTATCGATCAAGCTGTCCTCCATGGTCTTAAATCATCAAATTGCATTACTTGTGCTTATGGAACAACTTTACAGGGGCTTCCTCTGCCATCACCATCAGACCTATCTAGTGAGTTCTTGTGGAGTGGGTCGGATACCTAATTTGGCTGTAGCACATCAGCTAAATATAGAGGGGCAACTTAAGACCATATCAGCATTTCAACAATGGAATCCCATTCTCGTCTCTTCAATTGTTGAGAACACTCCCTACTTCTTCCTCTCTGAAACCTGCGGTCCAAATTGCCTCAAAATCTGCTCGCCATCAATTTTTCCGACGAATCTCGACCATGAATTCGAGATCGAAGCTAGATTCTAG

Protein sequence

MDNEQRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIAVVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTYLVSSCGVGRIPNLAVAHQLNIEGQLKTISAFQQWNPILVSSIVENTPYFFLSETCGPNCLKICSPSIFPTNLDHEFEIEARF
BLAST of Carg21159 vs. NCBI nr
Match: XP_022940478.1 (putative RNA methyltransferase At5g10620 [Cucurbita moschata])

HSP 1 Score: 283.1 bits (723), Expect = 8.8e-73
Identity = 147/155 (94.84%), Postives = 148/155 (95.48%), Query Frame = 0

Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 85

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN
Sbjct: 86  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 145

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           VSIKLSSMVLNHQIALLVLMEQLYR +     Q Y
Sbjct: 146 VSIKLSSMVLNHQIALLVLMEQLYRSWTILKGQKY 180

BLAST of Carg21159 vs. NCBI nr
Match: XP_023524400.1 (putative RNA methyltransferase At5g10620 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 278.1 bits (710), Expect = 2.8e-71
Identity = 144/155 (92.90%), Postives = 146/155 (94.19%), Query Frame = 0

Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDED+A
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDVA 85

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           VVNLLKSDDWVVMLDE GKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN
Sbjct: 86  VVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 145

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           VSIKLSSMVLNHQIALLVLMEQLY  +     Q Y
Sbjct: 146 VSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKY 180

BLAST of Carg21159 vs. NCBI nr
Match: XP_022982492.1 (putative RNA methyltransferase At5g10620 [Cucurbita maxima] >XP_022982493.1 putative RNA methyltransferase At5g10620 [Cucurbita maxima])

HSP 1 Score: 276.2 bits (705), Expect = 1.1e-70
Identity = 143/155 (92.26%), Postives = 146/155 (94.19%), Query Frame = 0

Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRN RD RAQVDDEDIA
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNTRDARAQVDDEDIA 85

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRL+FCIGGPYGHGQQLRQRAN
Sbjct: 86  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 145

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           VSIKLSSMVLNHQIA+LVLMEQLYR +     Q Y
Sbjct: 146 VSIKLSSMVLNHQIAVLVLMEQLYRSWTILKGQKY 180

BLAST of Carg21159 vs. NCBI nr
Match: XP_008466330.1 (PREDICTED: putative RNA methyltransferase At5g10620 [Cucumis melo] >XP_008466331.1 PREDICTED: putative RNA methyltransferase At5g10620 [Cucumis melo])

HSP 1 Score: 271.6 bits (693), Expect = 2.7e-69
Identity = 137/155 (88.39%), Postives = 147/155 (94.84%), Query Frame = 0

Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RAVPIRVLTVGKKRSRGVQLL+DEYIDKL NYC+VEDVQLRSNPRNARDP+AQ+DDED+A
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLVDEYIDKLKNYCHVEDVQLRSNPRNARDPKAQIDDEDVA 85

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRL+FCIGGPYGHGQ+LR+RAN
Sbjct: 86  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLSFCIGGPYGHGQKLRERAN 145

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           +SIKLSSMVLNHQIALLVLMEQLYR +     Q Y
Sbjct: 146 LSIKLSSMVLNHQIALLVLMEQLYRSWTILKGQKY 180

BLAST of Carg21159 vs. NCBI nr
Match: XP_022131264.1 (putative RNA methyltransferase At5g10620 isoform X2 [Momordica charantia])

HSP 1 Score: 268.5 bits (685), Expect = 2.3e-68
Identity = 136/155 (87.74%), Postives = 147/155 (94.84%), Query Frame = 0

Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RAVPIRVLTVGKKRSRGVQL++DEYI+KL +YCNVEDVQLRSNPRNARDPRAQVDDED+A
Sbjct: 30  RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA 89

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           VVNLLKSDDWVVMLDENGKE+SSE+MAELVADAGNTGASRL+FCIGGPYGHGQQLRQRAN
Sbjct: 90  VVNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 149

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           ++IKLSSMVLNHQIALLVLMEQLYR +     Q Y
Sbjct: 150 LTIKLSSMVLNHQIALLVLMEQLYRSWTILKGQKY 184

BLAST of Carg21159 vs. TAIR10
Match: AT5G10620.1 (methyltransferases)

HSP 1 Score: 214.2 bits (544), Expect = 9.2e-56
Identity = 106/155 (68.39%), Postives = 132/155 (85.16%), Query Frame = 0

Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RA+PIRV+TVGKKRS GV+LL+DEY  KL  YC+ ED  +RSNPRNA+D RAQV+DE++A
Sbjct: 37  RALPIRVITVGKKRSEGVRLLVDEYKIKLKPYCSFEDSLVRSNPRNAQDVRAQVEDEEVA 96

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           ++ L+ SDDWVV+LDE G++I SEQMAEL+ DAGN+GASR++FCIGG YGHG Q+R+RAN
Sbjct: 97  MMKLIGSDDWVVVLDERGRDIDSEQMAELLGDAGNSGASRISFCIGGAYGHGTQVRKRAN 156

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           V+I+LSSMVLNHQIAL+VLMEQLYR +     Q Y
Sbjct: 157 VTIRLSSMVLNHQIALVVLMEQLYRSWTILKGQNY 191

BLAST of Carg21159 vs. TAIR10
Match: AT5G10605.1 (methyltransferases)

HSP 1 Score: 57.4 bits (137), Expect = 1.4e-08
Identity = 28/36 (77.78%), Postives = 33/36 (91.67%), Query Frame = 0

Query: 111 GGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLME 147
           G  YGHG+QLR+RANV+I+LSSMVLNH IAL+VLME
Sbjct: 21  GVAYGHGRQLRKRANVAIRLSSMVLNHHIALVVLME 56

BLAST of Carg21159 vs. Swiss-Prot
Match: sp|Q9LXB4|Y5620_ARATH (Putative RNA methyltransferase At5g10620 OS=Arabidopsis thaliana OX=3702 GN=At5g10620 PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.7e-54
Identity = 106/155 (68.39%), Postives = 132/155 (85.16%), Query Frame = 0

Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RA+PIRV+TVGKKRS GV+LL+DEY  KL  YC+ ED  +RSNPRNA+D RAQV+DE++A
Sbjct: 37  RALPIRVITVGKKRSEGVRLLVDEYKIKLKPYCSFEDSLVRSNPRNAQDVRAQVEDEEVA 96

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           ++ L+ SDDWVV+LDE G++I SEQMAEL+ DAGN+GASR++FCIGG YGHG Q+R+RAN
Sbjct: 97  MMKLIGSDDWVVVLDERGRDIDSEQMAELLGDAGNSGASRISFCIGGAYGHGTQVRKRAN 156

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           V+I+LSSMVLNHQIAL+VLMEQLYR +     Q Y
Sbjct: 157 VTIRLSSMVLNHQIALVVLMEQLYRSWTILKGQNY 191

BLAST of Carg21159 vs. Swiss-Prot
Match: sp|A0M0D0|RLMH_GRAFK (Ribosomal RNA large subunit methyltransferase H OS=Gramella forsetii (strain KT0803) OX=411154 GN=rlmH PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 8.6e-19
Identity = 56/156 (35.90%), Postives = 87/156 (55.77%), Query Frame = 0

Query: 10  IRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVE-----DVQLRSNPRNARDPRAQVDDEDI 69
           I+++ +GK   + ++ LI  Y D+L +Y   E     D++   N     D   Q   E  
Sbjct: 3   IKLVCIGKTDKKELEALIKIYSDRLQHYIKFEFEIIPDLKKTKN----LDENQQKSKEGE 62

Query: 70  AVVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRA 129
            +++ +++ D+VV+LDENGK+ SSE  +E +    NTG  RL F IGGPYG  +++ +RA
Sbjct: 63  LILSGVQNSDFVVLLDENGKQFSSESFSEYIQKRMNTGLKRLIFVIGGPYGFSEEVYKRA 122

Query: 130 NVSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           +  I LS M  +HQ+  L   EQLYR F    ++ Y
Sbjct: 123 DSKISLSKMTFSHQMVRLFFTEQLYRAFTILKNEPY 154

BLAST of Carg21159 vs. Swiss-Prot
Match: sp|A0PXE8|RLMH_CLONN (Ribosomal RNA large subunit methyltransferase H OS=Clostridium novyi (strain NT) OX=386415 GN=rlmH PE=3 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 4.2e-18
Identity = 54/155 (34.84%), Postives = 87/155 (56.13%), Query Frame = 0

Query: 10  IRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQL--RSNPRNA--RDPRAQVDDEDIA 69
           I ++ VGK + + ++L IDEY  +L+ YC +  V+L     P NA  +D       E   
Sbjct: 3   ITIICVGKLKEKYLKLAIDEYSKRLSRYCKLNIVELNDEKTPDNASEKDELIIKQKEGSK 62

Query: 70  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 129
           ++  +K + +V+ LD NGK  SSE++AEL+ D G  G S +A  IGG  G  +++  RAN
Sbjct: 63  ILAHVKENMYVIALDLNGKMASSEELAELIGDLGLKGKSNIALVIGGSLGLSKEVLNRAN 122

Query: 130 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
             +  S M   HQ+  ++L+EQ+YR +  +  + Y
Sbjct: 123 YKLSFSKMTFPHQLMRVILLEQIYRAYKINAGEPY 157

BLAST of Carg21159 vs. Swiss-Prot
Match: sp|B2V1Q9|RLMH_CLOBA (Ribosomal RNA large subunit methyltransferase H OS=Clostridium botulinum (strain Alaska E43 / Type E3) OX=508767 GN=rlmH PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.2e-17
Identity = 53/155 (34.19%), Postives = 90/155 (58.06%), Query Frame = 0

Query: 10  IRVLTVGKKRSRGVQLLIDEYIDKLNNYC--NVEDVQLRSNPRNA--RDPRAQVDDEDIA 69
           I +++VGK + + ++  IDEY  +L+ YC  N+ ++Q    P NA  +D     D E   
Sbjct: 3   ITIISVGKLKEKYLKHAIDEYSKRLSRYCKLNIVELQDEQTPDNASEKDELIIKDKEGNK 62

Query: 70  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 129
           ++N +K + +V+ LD  GK ISSE++++ + + G  G S L F IGG  G  + + +R+N
Sbjct: 63  ILNSIKDNMYVITLDLKGKMISSEELSKFIDNCGVRGNSNLCFIIGGSLGLSEAVLKRSN 122

Query: 130 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
            S+  S M   HQ+  ++L+EQ+YR F   + + Y
Sbjct: 123 YSLCFSKMTFPHQLFRVMLLEQIYRAFRISNGEPY 157

BLAST of Carg21159 vs. Swiss-Prot
Match: sp|A7FZD1|RLMH_CLOB1 (Ribosomal RNA large subunit methyltransferase H OS=Clostridium botulinum (strain ATCC 19397 / Type A) OX=441770 GN=rlmH PE=3 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.6e-17
Identity = 51/147 (34.69%), Postives = 85/147 (57.82%), Query Frame = 0

Query: 10  IRVLTVGKKRSRGVQLLIDEYIDKLNNYC--NVEDVQLRSNPRNA--RDPRAQVDDEDIA 69
           I +++VGK + + ++  IDEY  +L+ YC  N+ +V     P NA  ++     + E   
Sbjct: 3   ISIISVGKIKEKFLKAAIDEYSKRLSKYCKLNIIEVADEKTPDNASLKEENIIKEKEGNL 62

Query: 70  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 129
           ++  +K + +V+ LD  GK I+SE+ ++L+ +   TG S +AF IGG  G  QQ+  RAN
Sbjct: 63  ILKHIKDNSFVIALDLKGKSITSEEFSDLIENCRLTGNSTIAFVIGGSLGLSQQVLSRAN 122

Query: 130 VSIKLSSMVLNHQIALLVLMEQLYRGF 153
             +  S M   HQ+  ++L+EQ+YR F
Sbjct: 123 YKLSFSKMTFPHQLFRVMLLEQVYRAF 149

BLAST of Carg21159 vs. TrEMBL
Match: tr|A0A1S3CQZ8|A0A1S3CQZ8_CUCME (putative RNA methyltransferase At5g10620 OS=Cucumis melo OX=3656 GN=LOC103503770 PE=3 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.8e-69
Identity = 137/155 (88.39%), Postives = 147/155 (94.84%), Query Frame = 0

Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RAVPIRVLTVGKKRSRGVQLL+DEYIDKL NYC+VEDVQLRSNPRNARDP+AQ+DDED+A
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLVDEYIDKLKNYCHVEDVQLRSNPRNARDPKAQIDDEDVA 85

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRL+FCIGGPYGHGQ+LR+RAN
Sbjct: 86  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLSFCIGGPYGHGQKLRERAN 145

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           +SIKLSSMVLNHQIALLVLMEQLYR +     Q Y
Sbjct: 146 LSIKLSSMVLNHQIALLVLMEQLYRSWTILKGQKY 180

BLAST of Carg21159 vs. TrEMBL
Match: tr|A0A0A0LGS0|A0A0A0LGS0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G881670 PE=3 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 4.3e-68
Identity = 136/155 (87.74%), Postives = 144/155 (92.90%), Query Frame = 0

Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RAVPIRVLTVGKKRSRGVQLL+DEYIDKL NYC+VEDVQLRSNPRNARDP+AQVDDED+A
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLVDEYIDKLKNYCHVEDVQLRSNPRNARDPKAQVDDEDVA 85

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           VVNLLK DDWVVMLDENGKE+SSE MAELVADAGNT ASRL+FCIGGPYGHGQQLRQRAN
Sbjct: 86  VVNLLKPDDWVVMLDENGKELSSELMAELVADAGNTSASRLSFCIGGPYGHGQQLRQRAN 145

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           +SIKLSSMVLNHQIALLVLMEQLYR +     Q Y
Sbjct: 146 LSIKLSSMVLNHQIALLVLMEQLYRSWTILKGQKY 180

BLAST of Carg21159 vs. TrEMBL
Match: tr|A0A2P4MHB7|A0A2P4MHB7_QUESU (Putative rna methyltransferase at5g10620 OS=Quercus suber OX=58331 GN=CFP56_17194 PE=3 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 6.7e-61
Identity = 118/155 (76.13%), Postives = 139/155 (89.68%), Query Frame = 0

Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           R VPIR+LTVGKKRS GVQL++DEY+ KLN+YC V+DVQ+RSNPRNA D RAQVDDED A
Sbjct: 27  RVVPIRILTVGKKRSPGVQLIVDEYVRKLNHYCTVDDVQIRSNPRNASDARAQVDDEDTA 86

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           ++N+++SDDWVVMLDENG++I SEQMAELV DAGNTGASRL+FCIGGPYGHG+QLR+RAN
Sbjct: 87  MMNIIRSDDWVVMLDENGQDIGSEQMAELVGDAGNTGASRLSFCIGGPYGHGRQLRERAN 146

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           +SIKLS++VLNHQIALLVLMEQLYR +     Q Y
Sbjct: 147 ISIKLSTLVLNHQIALLVLMEQLYRSWTILKGQKY 181

BLAST of Carg21159 vs. TrEMBL
Match: tr|A0A1R3GVA9|A0A1R3GVA9_COCAP (Putative SPOUT methyltransferase OS=Corchorus capsularis OX=210143 GN=CCACVL1_23110 PE=4 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 8.8e-61
Identity = 118/144 (81.94%), Postives = 135/144 (93.75%), Query Frame = 0

Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RA+PIR+LTVGK RS GVQLL+DEYI KL NYC+V+DVQ+RSNP+NARDPRAQVDDED A
Sbjct: 326 RALPIRILTVGKTRSPGVQLLVDEYIAKLKNYCHVDDVQIRSNPKNARDPRAQVDDEDTA 385

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           +VNL+KSDDWVV+LDE G +I SEQMA+L+ DAGNTGASRL+FCIGGPYGHGQQLR+RAN
Sbjct: 386 IVNLIKSDDWVVLLDERGVDIRSEQMADLLGDAGNTGASRLSFCIGGPYGHGQQLRERAN 445

Query: 126 VSIKLSSMVLNHQIALLVLMEQLY 150
           +SIKLSSMVLNHQIAL+VLMEQLY
Sbjct: 446 ISIKLSSMVLNHQIALVVLMEQLY 469

BLAST of Carg21159 vs. TrEMBL
Match: tr|A0A2I4GP49|A0A2I4GP49_9ROSI (putative RNA methyltransferase At5g10620 isoform X2 OS=Juglans regia OX=51240 GN=LOC109009592 PE=3 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 2.6e-60
Identity = 118/155 (76.13%), Postives = 139/155 (89.68%), Query Frame = 0

Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RA+PIR+LTVGKKRSRG QL++DEYI KL +YC+V+DVQ+RSNPRNA D RAQVDDED A
Sbjct: 33  RALPIRILTVGKKRSRGAQLVVDEYIAKLKHYCSVDDVQIRSNPRNAHDLRAQVDDEDSA 92

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           ++NL++ DDWVVMLDENG++I SEQMAELV DAGNTGASRL+FCIGGPYGHGQQLR+RAN
Sbjct: 93  IMNLIRPDDWVVMLDENGQDIGSEQMAELVGDAGNTGASRLSFCIGGPYGHGQQLRERAN 152

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           +SI+LS++VLNHQIALLVLMEQLYR +     Q Y
Sbjct: 153 LSIRLSTLVLNHQIALLVLMEQLYRSWTILKGQKY 187

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022940478.18.8e-7394.84putative RNA methyltransferase At5g10620 [Cucurbita moschata][more]
XP_023524400.12.8e-7192.90putative RNA methyltransferase At5g10620 [Cucurbita pepo subsp. pepo][more]
XP_022982492.11.1e-7092.26putative RNA methyltransferase At5g10620 [Cucurbita maxima] >XP_022982493.1 puta... [more]
XP_008466330.12.7e-6988.39PREDICTED: putative RNA methyltransferase At5g10620 [Cucumis melo] >XP_008466331... [more]
XP_022131264.12.3e-6887.74putative RNA methyltransferase At5g10620 isoform X2 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G10620.19.2e-5668.39methyltransferases[more]
AT5G10605.11.4e-0877.78methyltransferases[more]
Match NameE-valueIdentityDescription
sp|Q9LXB4|Y5620_ARATH1.7e-5468.39Putative RNA methyltransferase At5g10620 OS=Arabidopsis thaliana OX=3702 GN=At5g... [more]
sp|A0M0D0|RLMH_GRAFK8.6e-1935.90Ribosomal RNA large subunit methyltransferase H OS=Gramella forsetii (strain KT0... [more]
sp|A0PXE8|RLMH_CLONN4.2e-1834.84Ribosomal RNA large subunit methyltransferase H OS=Clostridium novyi (strain NT)... [more]
sp|B2V1Q9|RLMH_CLOBA1.2e-1734.19Ribosomal RNA large subunit methyltransferase H OS=Clostridium botulinum (strain... [more]
sp|A7FZD1|RLMH_CLOB13.6e-1734.69Ribosomal RNA large subunit methyltransferase H OS=Clostridium botulinum (strain... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3CQZ8|A0A1S3CQZ8_CUCME1.8e-6988.39putative RNA methyltransferase At5g10620 OS=Cucumis melo OX=3656 GN=LOC103503770... [more]
tr|A0A0A0LGS0|A0A0A0LGS0_CUCSA4.3e-6887.74Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G881670 PE=3 SV=1[more]
tr|A0A2P4MHB7|A0A2P4MHB7_QUESU6.7e-6176.13Putative rna methyltransferase at5g10620 OS=Quercus suber OX=58331 GN=CFP56_1719... [more]
tr|A0A1R3GVA9|A0A1R3GVA9_COCAP8.8e-6181.94Putative SPOUT methyltransferase OS=Corchorus capsularis OX=210143 GN=CCACVL1_23... [more]
tr|A0A2I4GP49|A0A2I4GP49_9ROSI2.6e-6076.13putative RNA methyltransferase At5g10620 isoform X2 OS=Juglans regia OX=51240 GN... [more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006364rRNA processing
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
Vocabulary: Molecular Function
TermDefinition
GO:0008168methyltransferase activity
Vocabulary: INTERPRO
TermDefinition
IPR029028Alpha/beta_knot_MTases
IPR003742RlmH-like
IPR029026tRNA_m1G_MTases_N
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006364 rRNA processing
cellular_component GO:0005737 cytoplasm
molecular_function GO:0008168 methyltransferase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg21159-RACarg21159-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029026tRNA (guanine-N1-)-methyltransferase, N-terminalGENE3DG3DSA:3.40.1280.10coord: 9..162
e-value: 2.3E-44
score: 152.8
IPR003742RNA methyltransferase RlmHPFAMPF02590SPOUT_MTasecoord: 10..160
e-value: 1.2E-40
score: 138.9
IPR003742RNA methyltransferase RlmHPANTHERPTHR33603FAMILY NOT NAMEDcoord: 9..160
IPR003742RNA methyltransferase RlmHHAMAPMF_0065823SrRNA_methyltr_Hcoord: 8..162
score: 20.449
IPR029028Alpha/beta knot methyltransferasesSUPERFAMILYSSF75217alpha/beta knotcoord: 10..160

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Carg21159Cucumber (Chinese Long) v2carcuB0583
Carg21159Silver-seed gourdcarcarB065
Carg21159Silver-seed gourdcarcarB511
Carg21159Silver-seed gourdcarcarB512
Carg21159Silver-seed gourdcarcarB513
Carg21159Cucumber (Gy14) v2carcgybB0557
Carg21159Cucumber (Gy14) v2carcgybB0558
Carg21159Cucumber (Gy14) v1carcgyB0628
Carg21159Cucumber (Gy14) v1carcgyB0631
Carg21159Cucurbita maxima (Rimu)carcmaB0767
Carg21159Cucurbita maxima (Rimu)carcmaB0768
Carg21159Cucurbita maxima (Rimu)carcmaB0770
Carg21159Cucurbita maxima (Rimu)carcmaB0772
Carg21159Cucurbita moschata (Rifu)carcmoB0746
Carg21159Cucurbita moschata (Rifu)carcmoB0748
Carg21159Cucurbita moschata (Rifu)carcmoB0750
Carg21159Cucurbita pepo (Zucchini)carcpeB0779
Carg21159Cucurbita pepo (Zucchini)carcpeB0782
Carg21159Cucurbita pepo (Zucchini)carcpeB0783
Carg21159Cucurbita pepo (Zucchini)carcpeB0784
Carg21159Wild cucumber (PI 183967)carcpiB0587
Carg21159Wild cucumber (PI 183967)carcpiB0589
Carg21159Cucumber (Chinese Long) v2carcuB0581
Carg21159Cucumber (Chinese Long) v3carcucB0588
Carg21159Cucumber (Chinese Long) v3carcucB0590
Carg21159Bottle gourd (USVL1VR-Ls)carlsiB499
Carg21159Bottle gourd (USVL1VR-Ls)carlsiB502
Carg21159Melon (DHL92) v3.5.1carmeB0556
Carg21159Melon (DHL92) v3.5.1carmeB0557
Carg21159Melon (DHL92) v3.6.1carmedB0562
Carg21159Melon (DHL92) v3.6.1carmedB0563
Carg21159Watermelon (Charleston Gray)carwcgB0536
Carg21159Watermelon (Charleston Gray)carwcgB0539
Carg21159Watermelon (97103) v1carwmB0547
Carg21159Watermelon (97103) v1carwmB0549
Carg21159Watermelon (97103) v2carwmbB0579
Carg21159Watermelon (97103) v2carwmbB0580
Carg21159Wax gourdcarwgoB0661
Carg21159Wax gourdcarwgoB0662