Carg18472 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg18472
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Adaptin ear-binding coat-associated protein 1 NECAP-1
Location	Cucurbita_argyrosperma_scaffold_161 : 443113 .. 446407 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCAGGGCCGTCAATTTATCAGACTTCTGTCTCCCCATAATAAGCCTAGGATGGTTAGACATCGAATCGAACGGTTGAAGTTTGTTATAACAATACGGTTGCAATTGGAACCGGCGCAGAGGATTACGGGTTACTTCGTCTATGTCCGGACCTTCAATCTTCTTTGAAGCGGAGAGGGCAACTGAAAGCGAGTTGTTTCTCCAGGCTACCAGTTTTCACATCCAATAACTCAGCTCATTTTCAATTCACCTGAAATTTCCATATTCATTTCGATTTTCTCAATTCCGGCTTTGGTTTCAACGGATTGCTGGCCGTATTTCTCCGATCTCGATTTTGATCTCACGATCTCTCGAATCTCTTTCTTATTTCTCCATGGAGGAGAAGCAGCAGAAGACGGCGAAGGATCAAGAACGCCAAGAGGATCACGAAGAGAATGAAGCAATTGAGCTTGTTCTCTTTCAAGTTTCTGAATGTTATGTCTATTTGGTGAGTAAATTATGCAAGTTTTTAGTTCTGATGCAGAAATCTGAGCTTTCTGTGGACTTCTTTTGCGATTAGCCAAATGTGAAGTAGTTGTTTGCACCATAGTTGATTATATACTGTTGGGAATTGCTGATATCATGTTTGGCTTTTTTTCTTCATTTTCTTACGTCCTATTCTACATTGTTAAATCATGCAATTGAGATATCTTAGTAATTAACATGTATGTTGCTACTGTTTGATAACTTGTTGGTGCGTGTAATTTGTGGTGCCGCGTTTCTGCATCCACATTTGGCCTCTTGGTGGGATAATGTTCATATGAATAATGCGAGAAAACTAATGTAATGTATCAGCTGTTTTTTCTGTACAAATACAACAAGATAGTTTCGTGTGTACGATAATATTAGGTTCTGTTGGTTATTTTCTGTCTTCAACTTACCTGTTAATTTTTTCTAGTAAACCTTATTGTCTTTTCTAATGATTTCCTGCAGATACCGCCAAGAAAAAGTGCAGCTTCCTACAGGTTTTTCCCTTGATTTACAATGGATAATAGTGTGAATTGCAATATGATCTCGATGTCGTACAGAAGCCTTGTTGTTGACTAATTTTATGTCCCCTAGATCAATTTGGTGATTTCATTATTTAGTTTTCCAACATAAATGTGTCTGCTGGTGACTTATAATTCCAATTTAAGTTAATATTGAGTATAATTGCTAAAAGAATTTGGAAGTACACACCAACGAGATGCCTGAAATTTAGCTAGATCTAAAAATATCTCCCAAGACGTGCACTTATCTTTGAAGAGCTCTATGCCCACTCAGTAGCTGTTCAAACATTGAATAATGGGAAAATGCTACATGTCATGTTTAATGTCTTAAAAATCCACTACATATGCTCCTATCCAAAAAAAATCCACTATGTATGTCGCCATCTATGACTTAGTTATGAAAATCCACTATATAGTCGCCCATAATAACTTAGTTTTCCACTTGTGCTCTGGTTACCTTAAGGGCTGATGAATGGGATGTGAATAAATGGGCCTGGGAGGGAAAACTGAAAGTCCTTAGCAAGGGAGAAGAATGCATTATCAGACTTGAAGATAAGGCTACAGGTGAGATCATCTCTTATAGTTAGTTGCTACAAAGCAAGTATTGTGGATTGGTAGTATTAAAAGGTAGGTGAATTATATTATTGCAGACGAGGTATATTTGGTGTTTCTAAAGACTAATAAGCTGTTTATTATTATAATAGGTGAATTATATGCTCGGGCATTCTTGAGAGAAGGGGAACAGCATCCAGTTGAAACTGTAATTGATAGCAGCAGGTTGGTTTCATTTTTAATTGAAGTCTAATCGATATACCAACTTTATCTTGTTTTAATGCTATATTTGAAGGTGCCTGATATAATGCTCAACTATTGCAGATATTTTGTTCTTCGCATAGAAGAGAATATTGGTGAGATGACCGTTTCTCTTGCCCACATTTTCCCATATTCTTTTAATTATCTCGTTATAGTACGCATCTGGATCACTTTTCTGTGTAATTTTCAGTTTTTGGCCCCCTCCCCCATCTCACTTGATCTTATGTTTTGTTTAATGAAGCTATATGTGGTGAAGTGTCATGAAATAACATAAAAGAATATCACTAAATGTTTCAATGAAGTAATTCTTTCCCGTAAAGTTTTCTGTCTACATTCTTGCTTGAGTGGCGCTGATATGGGTGCAATGGGGTATTTATTTGTGAAACATGCGACCAGCTAACACTTAATTTCTTGACGATAGAACAGTAGAGGTACAGGGGGGGAAATGGAGACTGTAGAACAGAATCAATTTGGATTGAATAATTAATAAATGTGAAAGAAATATATGTATACATATGCAGGTGGTCGCCTTCGGCACGCATTTATCGGCATAGGGTTTCGAGAAAGAACTGAGGCTTATGACTTCCAAGCAGCTTTGCATGATCATATGAAGTACCCTACCTTCTACTCTGCAATTATTCCATTTTCAAATAGAATTTTCTTGTGAAGTTGAATTATAATAGCATCCTTTTGTGATGATATGGTCTACCATTTGTAAATCTAATGCACTTTTTACAAACTTTTTTGAGGAAAGATATCTCAATAAAAAGAAGAGTGCAGAAGAGATGGAACAACAGTTCCAGAAAACATCCTCAGTTGATTACAGTCTGAAAGACGGCGAGACAATAGTACTTCAATTCAAAAGCAAAGTAGGCTGATTTTCTTTGCGTTGATATCTGCTTCCACACATTGGTTGTTTCATTGTGATTAATCTCATCTTTACACGTTCTTTTTTGTTACCAACAGAAAAGCACTAGCAACATCAAGAAGCCTTCTGAGCTAGATGTGAAGGTTTCCCCACCAGAAGAAGAGGGCAACACAAAAAAAGAGTCCATTTTTTGCATCAAACCACCTCCACCTCCTCCAGCTCTTCTTTCACCTGTTGCAGCCCAGAAGTCTCCTACGAATGTACCACCAAATATCAATCTGGACGATCCTTCCAAGCACAAGCTTGCAGAACCAGCAAAAGAAGACTCCAATGATGCTGAAGGTCAAAGCACACTTGATTTACCTGATGACGACTTTGGAAATTTCCAGGCAGCTGAATGATGGCATGGAACGTGTACATTCTCGAATACCCAAATCGATCTTCGGTTTTCAGTAGATCGCATGCTACAAATTGCTTAGATTTCACAAGAGATCTTATGTGTTGTTATTAACCTTGTTGTGAAATTAGCATGAAGAGTTATGATTATACCATGCCTCAGCGGTTCAGTTATAACTGTGATCATGCCAA

mRNA sequence

TCAGGGCCGTCAATTTATCAGACTTCTGTCTCCCCATAATAAGCCTAGGATGGTTAGACATCGAATCGAACGGTTGAAGTTTGTTATAACAATACGGTTGCAATTGGAACCGGCGCAGAGGATTACGGGTTACTTCGTCTATGTCCGGACCTTCAATCTTCTTTGAAGCGGAGAGGGCAACTGAAAGCGAGTTGTTTCTCCAGGCTACCAGTTTTCACATCCAATAACTCAGCTCATTTTCAATTCACCTGAAATTTCCATATTCATTTCGATTTTCTCAATTCCGGCTTTGGTTTCAACGGATTGCTGGCCGTATTTCTCCGATCTCGATTTTGATCTCACGATCTCTCGAATCTCTTTCTTATTTCTCCATGGAGGAGAAGCAGCAGAAGACGGCGAAGGATCAAGAACGCCAAGAGGATCACGAAGAGAATGAAGCAATTGAGCTTGTTCTCTTTCAAGTTTCTGAATGTTATGTCTATTTGATACCGCCAAGAAAAAGTGCAGCTTCCTACAGGGCTGATGAATGGGATGTGAATAAATGGGCCTGGGAGGGAAAACTGAAAGTCCTTAGCAAGGGAGAAGAATGCATTATCAGACTTGAAGATAAGGCTACAGGTGAATTATATGCTCGGGCATTCTTGAGAGAAGGGGAACAGCATCCAGTTGAAACTGTAATTGATAGCAGCAGATATTTTGTTCTTCGCATAGAAGAGAATATTGGTGAGATGACCGTTTCTCTTGCCCACATTTTCCCATATTCTTTTAATTATCTCGTTATATGGGGTATTTATTTGTGAAACATGCGACCAGCTAACACTTAATTTCTTGACGATAGAACAGTAGAGGTACAGAACAGAATCAATTTGGATTGAATAATTAATAAATGTGAAAGAAATATATGTATACATATGCAGGTGGTCGCCTTCGGCACGCATTTATCGGCATAGGGTTTCGAGAAAGAACTGAGGCTTATGACTTCCAAGCAGCTTTGCATGATCATATGAAATATCTCAATAAAAAGAAGAGTGCAGAAGAGATGGAACAACAGTTCCAGAAAACATCCTCAGTTGATTACAGTCTGAAAGACGGCGAGACAATAGTACTTCAATTCAAAAGCAAAAAAAGCACTAGCAACATCAAGAAGCCTTCTGAGCTAGATGTGAAGGTTTCCCCACCAGAAGAAGAGGGCAACACAAAAAAAGAGTCCATTTTTTGCATCAAACCACCTCCACCTCCTCCAGCTCTTCTTTCACCTGTTGCAGCCCAGAAGTCTCCTACGAATGTACCACCAAATATCAATCTGGACGATCCTTCCAAGCACAAGCTTGCAGAACCAGCAAAAGAAGACTCCAATGATGCTGAAGGTCAAAGCACACTTGATTTACCTGATGACGACTTTGGAAATTTCCAGGCAGCTGAATGATGGCATGGAACGTGTACATTCTCGAATACCCAAATCGATCTTCGGTTTTCAGTAGATCGCATGCTACAAATTGCTTAGATTTCACAAGAGATCTTATGTGTTGTTATTAACCTTGTTGTGAAATTAGCATGAAGAGTTATGATTATACCATGCCTCAGCGGTTCAGTTATAACTGTGATCATGCCAA

Coding sequence (CDS)

ATGTATACATATGCAGGTGGTCGCCTTCGGCACGCATTTATCGGCATAGGGTTTCGAGAAAGAACTGAGGCTTATGACTTCCAAGCAGCTTTGCATGATCATATGAAATATCTCAATAAAAAGAAGAGTGCAGAAGAGATGGAACAACAGTTCCAGAAAACATCCTCAGTTGATTACAGTCTGAAAGACGGCGAGACAATAGTACTTCAATTCAAAAGCAAAAAAAGCACTAGCAACATCAAGAAGCCTTCTGAGCTAGATGTGAAGGTTTCCCCACCAGAAGAAGAGGGCAACACAAAAAAAGAGTCCATTTTTTGCATCAAACCACCTCCACCTCCTCCAGCTCTTCTTTCACCTGTTGCAGCCCAGAAGTCTCCTACGAATGTACCACCAAATATCAATCTGGACGATCCTTCCAAGCACAAGCTTGCAGAACCAGCAAAAGAAGACTCCAATGATGCTGAAGGTCAAAGCACACTTGATTTACCTGATGACGACTTTGGAAATTTCCAGGCAGCTGAATGA

Protein sequence

MYTYAGGRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGETIVLQFKSKKSTSNIKKPSELDVKVSPPEEEGNTKKESIFCIKPPPPPPALLSPVAAQKSPTNVPPNINLDDPSKHKLAEPAKEDSNDAEGQSTLDLPDDDFGNFQAAE

Homology

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BLAST of Carg18472 vs. NCBI nr
Match: XP_023512594.1 (uncharacterized protein LOC111777291 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 261.9 bits (668), Expect = 1.5e-66
Identity = 169/169 (100.00%), Postives = 169/169 (100.00%), Query Frame = 0


Query: 6   GGRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGE 65
           GGRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGE
Sbjct: 424 GGRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGE 483

Query: 66  TIVLQFKSKKSTSNIKKPSELDVKVSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQKS 125
           TIVLQFKSKKSTSNIKKPSELDVKVSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQKS
Sbjct: 484 TIVLQFKSKKSTSNIKKPSELDVKVSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQKS 543

Query: 126 PTNVPPNINLDDPSKHKLAEPAKEDSNDAEGQSTLDLPDDDFGNFQAAE 175
           PTNVPPNINLDDPSKHKLAEPAKEDSNDAEGQSTLDLPDDDFGNFQAAE
Sbjct: 544 PTNVPPNINLDDPSKHKLAEPAKEDSNDAEGQSTLDLPDDDFGNFQAAE 592

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BLAST of Carg18472 vs. NCBI nr
Match: XP_022944659.1 (uncharacterized protein At1g03900-like [Cucurbita moschata])

HSP 1 Score: 260.0 bits (663), Expect = 5.8e-66
Identity = 167/169 (98.82%), Postives = 169/169 (100.00%), Query Frame = 0


Query: 6   GGRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGE 65
           GGRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGE
Sbjct: 119 GGRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGE 178

Query: 66  TIVLQFKSKKSTSNIKKPSELDVKVSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQKS 125
           TIVLQFKSKKSTSNIKKPSELDVKVSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQKS
Sbjct: 179 TIVLQFKSKKSTSNIKKPSELDVKVSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQKS 238

Query: 126 PTNVPPNINLDDPSKHKLAEPAKEDSNDAEGQSTLDLPDDDFGNFQAAE 175
           PTNVPPNINLDDPSKHK+AEPA+EDSNDAEGQSTLDLPDDDFGNFQAAE
Sbjct: 239 PTNVPPNINLDDPSKHKIAEPAREDSNDAEGQSTLDLPDDDFGNFQAAE 287

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BLAST of Carg18472 vs. NCBI nr
Match: XP_022149778.1 (uncharacterized protein At1g03900 [Momordica charantia])

HSP 1 Score: 229.6 bits (584), Expect = 8.3e-57
Identity = 129/168 (76.79%), Postives = 135/168 (80.36%), Query Frame = 0


Query: 6   GGRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGE 65
           GGR RHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQ TSSVDYSLKDGE
Sbjct: 117 GGRRRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGE 176

Query: 66  TIVLQFKSKKSTSNIKKPSELDVKVSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQKS 125
           TIVLQ KSKKSTSNIKK SELDVKVS                  XXXXX       AQKS
Sbjct: 177 TIVLQLKSKKSTSNIKKCSELDVKVSSSEEDGSTKKESIFCIKPXXXXXSPLSPVEAQKS 236

Query: 126 PTNVPPNINLDDPSKHKLAEPAKEDSNDAEGQSTLDLPDDDFGNFQAA 174
           PTN+PPNINL++PSKHK +EPAKEDSN AE QSTLD+PDDDFG+FQ A
Sbjct: 237 PTNLPPNINLEEPSKHKTSEPAKEDSNPAEAQSTLDVPDDDFGDFQTA 284

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BLAST of Carg18472 vs. NCBI nr
Match: XP_004150324.1 (PREDICTED: uncharacterized protein At1g03900 isoform X2 [Cucumis sativus] >KGN47271.1 hypothetical protein Csa_6G239700 [Cucumis sativus])

HSP 1 Score: 215.7 bits (548), Expect = 1.2e-52
Identity = 127/168 (75.60%), Postives = 137/168 (81.55%), Query Frame = 0


Query: 7   GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGET 66
           GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQ TSSVDYSLKDGET
Sbjct: 119 GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGET 178

Query: 67  IVLQFKSKKSTSNIKKPSELD-VKVSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQKS 126
           IVLQ KS+KSTSN+KK +ELD VKVS                  XXXXXXXXX   +QKS
Sbjct: 179 IVLQLKSRKSTSNVKKSTELDHVKVSSLEEEGNTKKDSIFCIRPXXXXXXXXXPVASQKS 238

Query: 127 PTNVPPNINLDDPSKHKLAEPAKEDSNDAEGQSTLDLPDDDFGNFQAA 174
           P N+PPNINL++PSK K  EPAKED+NDAE QSTLD+PDDDFG+FQ A
Sbjct: 239 PKNLPPNINLEEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTA 286

[Close]

BLAST of Carg18472 vs. NCBI nr
Match: XP_011657208.1 (PREDICTED: uncharacterized protein At1g03900 isoform X1 [Cucumis sativus])

HSP 1 Score: 215.7 bits (548), Expect = 1.2e-52
Identity = 127/168 (75.60%), Postives = 137/168 (81.55%), Query Frame = 0


Query: 7   GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGET 66
           GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQ TSSVDYSLKDGET
Sbjct: 151 GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGET 210

Query: 67  IVLQFKSKKSTSNIKKPSELD-VKVSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQKS 126
           IVLQ KS+KSTSN+KK +ELD VKVS                  XXXXXXXXX   +QKS
Sbjct: 211 IVLQLKSRKSTSNVKKSTELDHVKVSSLEEEGNTKKDSIFCIRPXXXXXXXXXPVASQKS 270

Query: 127 PTNVPPNINLDDPSKHKLAEPAKEDSNDAEGQSTLDLPDDDFGNFQAA 174
           P N+PPNINL++PSK K  EPAKED+NDAE QSTLD+PDDDFG+FQ A
Sbjct: 271 PKNLPPNINLEEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTA 318

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BLAST of Carg18472 vs. TAIR10
Match: AT3G58600.1 (Adaptin ear-binding coat-associated protein 1 NECAP-1)

HSP 1 Score: 121.3 bits (303), Expect = 5.8e-28
Identity = 60/79 (75.95%), Postives = 68/79 (86.08%), Query Frame = 0


Query: 7   GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGET 66
           GR+RHAFIG+GFRERTEAYDFQAALHDHMKYLNKKK+AEEMEQ +Q TSSVDYSLK+GET
Sbjct: 127 GRVRHAFIGLGFRERTEAYDFQAALHDHMKYLNKKKTAEEMEQHYQNTSSVDYSLKEGET 186

Query: 67  IVLQFKSKKSTSNIKKPSE 86
           IVLQ K++    +  K  E
Sbjct: 187 IVLQLKNRSDKDSKSKTVE 205

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BLAST of Carg18472 vs. TAIR10
Match: AT1G03900.1 (non-intrinsic ABC protein 4)

HSP 1 Score: 56.6 bits (135), Expect = 1.8e-08
Identity = 30/66 (45.45%), Postives = 42/66 (63.64%), Query Frame = 0


Query: 7   GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAE-----EMEQQFQKTSSVDYSL 66
           GR ++AFIG+GF ER EA+DF  AL DH KY+ ++K  E     E +       +V++ L
Sbjct: 105 GRGKYAFIGLGFAERNEAFDFNVALSDHEKYVRREKEKETGETSESDNHIDIHPAVNHRL 164

Query: 67  KDGETI 68
           K+GETI
Sbjct: 165 KEGETI 170

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BLAST of Carg18472 vs. Swiss-Prot
Match: sp|Q681Q7|Y1390_ARATH (Uncharacterized protein At1g03900 OS=Arabidopsis thaliana OX=3702 GN=At1g03900 PE=2 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 3.2e-07
Identity = 30/66 (45.45%), Postives = 42/66 (63.64%), Query Frame = 0


Query: 7   GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAE-----EMEQQFQKTSSVDYSL 66
           GR ++AFIG+GF ER EA+DF  AL DH KY+ ++K  E     E +       +V++ L
Sbjct: 105 GRGKYAFIGLGFAERNEAFDFNVALSDHEKYVRREKEKETGETSESDNHIDIHPAVNHRL 164

Query: 67  KDGETI 68
           K+GETI
Sbjct: 165 KEGETI 170

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BLAST of Carg18472 vs. Swiss-Prot
Match: sp|Q5E9Q4|NECP2_BOVIN (Adaptin ear-binding coat-associated protein 2 OS=Bos taurus OX=9913 GN=NECAP2 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 6.0e-06
Identity = 28/69 (40.58%), Postives = 40/69 (57.97%), Query Frame = 0


Query: 7   GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGET 66
           G  R AFIGIGF +R +A+DF  AL DH K++ ++    +  Q   +   +D S K+G+T
Sbjct: 98  GNGRRAFIGIGFGDRGDAFDFNVALQDHFKWVKQQCEFAKQAQNPDQGPKLDLSFKEGQT 157

Query: 67  IVLQFKSKK 76
           I L   S K
Sbjct: 158 IKLNIASMK 166

[Close]

BLAST of Carg18472 vs. Swiss-Prot
Match: sp|Q3T093|NECP1_BOVIN (Adaptin ear-binding coat-associated protein 1 OS=Bos taurus OX=9913 GN=NECAP1 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.0e-05
Identity = 24/63 (38.10%), Postives = 37/63 (58.73%), Query Frame = 0


Query: 7   GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGET 66
           G  R AFIGIGF +R +A+DF  +L DH K++ ++    +  Q+      +D   K+G+T
Sbjct: 99  GTGRSAFIGIGFSDRGDAFDFNVSLQDHFKWVKQESEISKESQEMDSRPKLDLGFKEGQT 158

Query: 67  IVL 70
           I L
Sbjct: 159 IKL 161

[Close]

BLAST of Carg18472 vs. Swiss-Prot
Match: sp|Q8NC96|NECP1_HUMAN (Adaptin ear-binding coat-associated protein 1 OS=Homo sapiens OX=9606 GN=NECAP1 PE=1 SV=2)

HSP 1 Score: 51.2 bits (121), Expect = 1.3e-05
Identity = 24/63 (38.10%), Postives = 37/63 (58.73%), Query Frame = 0


Query: 7   GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGET 66
           G  R AFIGIGF +R +A+DF  +L DH K++ ++    +  Q+      +D   K+G+T
Sbjct: 99  GTGRSAFIGIGFTDRGDAFDFNVSLQDHFKWVKQESEISKESQEMDARPKLDLGFKEGQT 158

Query: 67  IVL 70
           I L
Sbjct: 159 IKL 161

[Close]

BLAST of Carg18472 vs. Swiss-Prot
Match: sp|Q9CR95|NECP1_MOUSE (Adaptin ear-binding coat-associated protein 1 OS=Mus musculus OX=10090 GN=Necap1 PE=1 SV=2)

HSP 1 Score: 51.2 bits (121), Expect = 1.3e-05
Identity = 24/63 (38.10%), Postives = 37/63 (58.73%), Query Frame = 0


Query: 7   GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGET 66
           G  R AFIGIGF +R +A+DF  +L DH K++ ++    +  Q+      +D   K+G+T
Sbjct: 99  GTGRSAFIGIGFTDRGDAFDFNVSLQDHFKWVKQETEISKESQEMDNRPKLDLGFKEGQT 158

Query: 67  IVL 70
           I L
Sbjct: 159 IKL 161

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BLAST of Carg18472 vs. TrEMBL
Match: tr|A0A0A0KHL0|A0A0A0KHL0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G239700 PE=4 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 8.3e-53
Identity = 127/168 (75.60%), Postives = 137/168 (81.55%), Query Frame = 0


Query: 7   GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGET 66
           GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQ TSSVDYSLKDGET
Sbjct: 119 GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGET 178

Query: 67  IVLQFKSKKSTSNIKKPSELD-VKVSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQKS 126
           IVLQ KS+KSTSN+KK +ELD VKVS                  XXXXXXXXX   +QKS
Sbjct: 179 IVLQLKSRKSTSNVKKSTELDHVKVSSLEEEGNTKKDSIFCIRPXXXXXXXXXPVASQKS 238

Query: 127 PTNVPPNINLDDPSKHKLAEPAKEDSNDAEGQSTLDLPDDDFGNFQAA 174
           P N+PPNINL++PSK K  EPAKED+NDAE QSTLD+PDDDFG+FQ A
Sbjct: 239 PKNLPPNINLEEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTA 286

[Close]

BLAST of Carg18472 vs. TrEMBL
Match: tr|A0A1S3C3B1|A0A1S3C3B1_CUCME (uncharacterized protein At1g03900 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496372 PE=4 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.0e-50
Identity = 141/168 (83.93%), Postives = 149/168 (88.69%), Query Frame = 0


Query: 7   GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGET 66
           GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQ TSSVDYSLKDGET
Sbjct: 149 GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGET 208

Query: 67  IVLQFKSKKSTSNIKKPSELD-VKVSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQKS 126
           IVLQ KSKKSTSNIKK +ELD VKVS XXXXXXXXXXXXXXXXXXXXXXXX     +QKS
Sbjct: 209 IVLQLKSKKSTSNIKKSTELDHVKVSXXXXXXXXXXXXXXXXXXXXXXXXXLSPVASQKS 268

Query: 127 PTNVPPNINLDDPSKHKLAEPAKEDSNDAEGQSTLDLPDDDFGNFQAA 174
           P  +PPNINL++PSK K   P KED+NDAE QSTLD+PDDDFG+FQ A
Sbjct: 269 PKYLPPNINLEEPSKKKDLVPEKEDTNDAEVQSTLDIPDDDFGDFQTA 316

[Close]

BLAST of Carg18472 vs. TrEMBL
Match: tr|A0A1S3C3X0|A0A1S3C3X0_CUCME (uncharacterized protein At1g03900 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496372 PE=4 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.0e-50
Identity = 141/168 (83.93%), Postives = 149/168 (88.69%), Query Frame = 0


Query: 7   GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGET 66
           GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQ TSSVDYSLKDGET
Sbjct: 119 GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGET 178

Query: 67  IVLQFKSKKSTSNIKKPSELD-VKVSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQKS 126
           IVLQ KSKKSTSNIKK +ELD VKVS XXXXXXXXXXXXXXXXXXXXXXXX     +QKS
Sbjct: 179 IVLQLKSKKSTSNIKKSTELDHVKVSXXXXXXXXXXXXXXXXXXXXXXXXXLSPVASQKS 238

Query: 127 PTNVPPNINLDDPSKHKLAEPAKEDSNDAEGQSTLDLPDDDFGNFQAA 174
           P  +PPNINL++PSK K   P KED+NDAE QSTLD+PDDDFG+FQ A
Sbjct: 239 PKYLPPNINLEEPSKKKDLVPEKEDTNDAEVQSTLDIPDDDFGDFQTA 286

[Close]

BLAST of Carg18472 vs. TrEMBL
Match: tr|A0A2P6RUH5|A0A2P6RUH5_ROSCH (Putative PH domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2g0129091 PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 3.2e-36
Identity = 118/169 (69.82%), Postives = 134/169 (79.29%), Query Frame = 0


Query: 7   GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGET 66
           GRLRHAFIG+GFRERTEAYDFQAALHDHMKYLNKKK+AEEMEQ+FQ+TSSVDYSLK+GET
Sbjct: 114 GRLRHAFIGLGFRERTEAYDFQAALHDHMKYLNKKKTAEEMEQKFQQTSSVDYSLKEGET 173

Query: 67  IVLQFKSKKSTSNIKKPSELDVKVSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQKSP 126
           +VLQ KSK S     K SE  V  SPXXXXXXXXXXXXXXXXXXXXXX        QKSP
Sbjct: 174 LVLQLKSKSSEGVKSKLSEQGVSNSPXXXXXXXXXXXXXXXXXXXXXXPLSPSTNVQKSP 233

Query: 127 TNVPPNINLDDPSK--HKLAEPAKEDSNDAEGQSTLDLPDDDFGNFQAA 174
           +N PPN+ L+  SK    +A+ +KE  N +  QST ++PDDDFG+FQAA
Sbjct: 234 SN-PPNLALEGASKLPESMADDSKE-MNTSANQSTQEIPDDDFGDFQAA 280

[Close]

BLAST of Carg18472 vs. TrEMBL
Match: tr|A0A2P6RUC0|A0A2P6RUC0_ROSCH (Putative PH domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2g0128491 PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 3.2e-36
Identity = 118/169 (69.82%), Postives = 134/169 (79.29%), Query Frame = 0


Query: 7   GRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGET 66
           GRLRHAFIG+GFRERTEAYDFQAALHDHMKYLNKKK+AEEMEQ+FQ+TSSVDYSLK+GET
Sbjct: 102 GRLRHAFIGLGFRERTEAYDFQAALHDHMKYLNKKKTAEEMEQKFQQTSSVDYSLKEGET 161

Query: 67  IVLQFKSKKSTSNIKKPSELDVKVSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQKSP 126
           +VLQ KSK S     K SE  V  SPXXXXXXXXXXXXXXXXXXXXXX        QKSP
Sbjct: 162 LVLQLKSKSSEGVKSKLSEQGVSNSPXXXXXXXXXXXXXXXXXXXXXXPLSPSTNVQKSP 221

Query: 127 TNVPPNINLDDPSK--HKLAEPAKEDSNDAEGQSTLDLPDDDFGNFQAA 174
           +N PPN+ L+  SK    +A+ +KE  N +  QST ++PDDDFG+FQAA
Sbjct: 222 SN-PPNLALEGASKLPESMADDSKE-MNTSANQSTQEIPDDDFGDFQAA 268

The following BLAST results are available for this feature:

BLAST of Carg18472 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023512594.1	1.5e-66	100.00	uncharacterized protein LOC111777291 [Cucurbita pepo subsp. pepo]	[more]
XP_022944659.1	5.8e-66	98.82	uncharacterized protein At1g03900-like [Cucurbita moschata]	[more]
XP_022149778.1	8.3e-57	76.79	uncharacterized protein At1g03900 [Momordica charantia]	[more]
XP_004150324.1	1.2e-52	75.60	PREDICTED: uncharacterized protein At1g03900 isoform X2 [Cucumis sativus] >KGN47...	[more]
XP_011657208.1	1.2e-52	75.60	PREDICTED: uncharacterized protein At1g03900 isoform X1 [Cucumis sativus]	[more]

BLAST of Carg18472 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G58600.1	5.8e-28	75.95	Adaptin ear-binding coat-associated protein 1 NECAP-1	[more]
AT1G03900.1	1.8e-08	45.45	non-intrinsic ABC protein 4	[more]

BLAST of Carg18472 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q681Q7\|Y1390_ARATH	3.2e-07	45.45	Uncharacterized protein At1g03900 OS=Arabidopsis thaliana OX=3702 GN=At1g03900 P...	[more]
sp\|Q5E9Q4\|NECP2_BOVIN	6.0e-06	40.58	Adaptin ear-binding coat-associated protein 2 OS=Bos taurus OX=9913 GN=NECAP2 PE...	[more]
sp\|Q3T093\|NECP1_BOVIN	1.0e-05	38.10	Adaptin ear-binding coat-associated protein 1 OS=Bos taurus OX=9913 GN=NECAP1 PE...	[more]
sp\|Q8NC96\|NECP1_HUMAN	1.3e-05	38.10	Adaptin ear-binding coat-associated protein 1 OS=Homo sapiens OX=9606 GN=NECAP1 ...	[more]
sp\|Q9CR95\|NECP1_MOUSE	1.3e-05	38.10	Adaptin ear-binding coat-associated protein 1 OS=Mus musculus OX=10090 GN=Necap1...	[more]

BLAST of Carg18472 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KHL0\|A0A0A0KHL0_CUCSA	8.3e-53	75.60	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G239700 PE=4 SV=1	[more]
tr\|A0A1S3C3B1\|A0A1S3C3B1_CUCME	1.0e-50	83.93	uncharacterized protein At1g03900 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349...	[more]
tr\|A0A1S3C3X0\|A0A1S3C3X0_CUCME	1.0e-50	83.93	uncharacterized protein At1g03900 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349...	[more]
tr\|A0A2P6RUH5\|A0A2P6RUH5_ROSCH	3.2e-36	69.82	Putative PH domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr...	[more]
tr\|A0A2P6RUC0\|A0A2P6RUC0_ROSCH	3.2e-36	69.82	Putative PH domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0006897	endocytosis

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR012466	NECAP_PHear
IPR011993	PH-like_dom_sf

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006897	endocytosis
cellular_component	GO:0016020	membrane
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18472-RA	Carg18472-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011993	PH-like domain superfamily	GENE3D	G3DSA:2.30.29.30		coord: 3..41 e-value: 4.7E-9 score: 38.1
IPR012466	NECAP, PHear domain	PFAM	PF07933	DUF1681	coord: 8..71 e-value: 4.5E-16 score: 58.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 78..102
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 72..174
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 138..155
None	No IPR available	PANTHER	PTHR12847:SF4	ADAPTIN EAR-BINDING COAT-ASSOCIATED PROTEIN 1 NECAP-1	coord: 7..173
None	No IPR available	PANTHER	PTHR12847	ATP-BINDING CASSETTE ABC TRANSPORTER-RELATED	coord: 7..173
None	No IPR available	SUPERFAMILY	SSF50729	PH domain-like	coord: 7..40

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg18472	Cla013530	Watermelon (97103) v1	carwmB0412
Carg18472	MELO3C019760	Melon (DHL92) v3.5.1	carmeB0418
Carg18472	ClCG02G017940	Watermelon (Charleston Gray)	carwcgB0408
Carg18472	CmaCh02G000780	Cucurbita maxima (Rimu)	carcmaB0577
Carg18472	CmoCh02G000760	Cucurbita moschata (Rifu)	carcmoB0561
Carg18472	CmoCh20G002410	Cucurbita moschata (Rifu)	carcmoB0559
Carg18472	Lsi10G015850	Bottle gourd (USVL1VR-Ls)	carlsiB377
Carg18472	Cp4.1LG05g15250	Cucurbita pepo (Zucchini)	carcpeB0581
Carg18472	Cla97C02G043720	Watermelon (97103) v2	carwmbB0438
Carg18472	Bhi10G000823	Wax gourd	carwgoB0500

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg18472	Carg13605	Silver-seed gourd	carcarB032

The following block(s) are covering this gene:


Gene	Organism	Block
Carg18472	Cucurbita maxima (Rimu)	carcmaB0574
Carg18472	Cucurbita maxima (Rimu)	carcmaB0575
Carg18472	Cucurbita moschata (Rifu)	carcmoB0558
Carg18472	Cucurbita pepo (Zucchini)	carcpeB0579
Carg18472	Melon (DHL92) v3.5.1	carmeB0421
Carg18472	Melon (DHL92) v3.6.1	carmedB0422