Carg18375 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg18375
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Gibberellin 2-beta-dioxygenase
Location	Cucurbita_argyrosperma_scaffold_106 : 370581 .. 372289 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCTTGTTCACATCCTCTCAATGGCTTCCTCAGTTGATCACAATAAGTTACCAAACATCTGCGGCGGCGCCACCGCAGCTCCTCCGCCCACTCCTTCCTCACAAACCAACCACCTCTCCACCTCTGCCGCTGCCGAAGCTCTCTCCAGGCTCCTCCACCGCCTCCCCCCCAAACTCTCTCTTCCTACGCGCCGTTCCTCGTCCGTTATATCGCCACCAACGATTTCCTTCTCAGATTCTCCCGATGACGAGTTTTCCAGTAGGCTACTCTCCGCCGTCTCAGAATTCGGCTTCTTCCAAATCACTGATCATAAAATATCCTCTCATCTCGTTCTCTCAGCCGAGTCGGAATCTGCCGCTCTATTCGACATTCAGGCGGAGAAGAAGGAAGCACTGTTTCCGAAGGACTGGGGATTCATAGGCGACGAAGACGAAGAATCGGACGGAGCCGGTGAATCGTTCTGCTTCGATCCGAGGCTGTGCTTGCCTTATTCCGACGAGATTTGTTTACAAGCTTTGCAAGAGCTCGTTCCTGCGATGGAGTATCTCGGACTGAAGATCGTGGAGCACCTATTTCGTGCGATTGGCCTCGAGAATCCAATCGGTGAGGATCGAAGCAGATTCCGGTCGCTGATATGGATATCGAAAGGTTCTCGAAGCGCTGAGCCGGCAGTGACCTGTGGATTTTACCCATACATTGTTGGATTGCATTATCAGAGCAAGAACCAGAGGTGTTCTGTATTAGGAGATTCCGGCTGGGTTGCGACGGCGGCAACGGCGGCGGATTCAGTGATGGTTTCAATCGGAGATATTGCGCAAGTAAGAAATAAACGCTGTCGTTTTGTTAATTTAATTTCTTTTTTTAAAATGAATAGAAAATTATAATTAATGGGTAAGGATATTTGATAATTAATAATTAAAAAGCTGTTAGATTGCACGAACTGAGAGGCATTGGTCACGTGCGTTCATTCGTGACAAATATGCTGCTTGTTTCACAAATCCACACAAACCTAATTTCTCGAAATTATTGATTTAATATTCTAAAAAGAAATTATTAGAGGAAGAAAAATTTAGCATTAAAATTTTGAAAATGTATTTTTAAAATAAAATTATTTCCGATTGTTTATCAAAATTTGTAGGTGTGGAGCAATGGAAAGGTAAAGAAAGTGAGAGGCAGGGCAGTAGCGGCGGCGGCTGACGGCAGCGACAACTCGGGCACCATCTCAATATCACTACTCATTACTCTTCCTTTGGATACCCAAGTCTCTCCTCTTCTTCCACCGACCGACGAAAACACAGACGCAGAAGCAGAAGAGGACGGCGGCGGCGGCAACGAGGCAGAGAAGAAAACGGCGGTATTTCGTTCGTTTAGCTTTGAAGAGTACGCGTGGAAAGTGTATCAGAATCGGTGCTTTCTGCTCAGAGATCCGCTGGCCGGATACCGAATCTGAATCGATGGATCGGAGAATTGCCCCATTGTTGATCAATCGACCCATTGAATTCCCACGGTTTTCTGCAGAGGCGTTGAGTACGTAGCTCTACTTTTTCTTTTTTCTTGTTTATTTTGAACAGTTTTTGGATTATTCTTGCGAGTGCTGTGTGATTTTGTTACAAATTTGAATAATCCCAAAAACAATATATTTAGATATTATATGGATAATCAACTTCAGGTTCATTTTTCTTTGAGAAATTTTGTGTTGGGTTTGAATT

mRNA sequence

TTCTTGTTCACATCCTCTCAATGGCTTCCTCAGTTGATCACAATAAGTTACCAAACATCTGCGGCGGCGCCACCGCAGCTCCTCCGCCCACTCCTTCCTCACAAACCAACCACCTCTCCACCTCTGCCGCTGCCGAAGCTCTCTCCAGGCTCCTCCACCGCCTCCCCCCCAAACTCTCTCTTCCTACGCGCCGTTCCTCGTCCGTTATATCGCCACCAACGATTTCCTTCTCAGATTCTCCCGATGACGAGTTTTCCAGTAGGCTACTCTCCGCCGTCTCAGAATTCGGCTTCTTCCAAATCACTGATCATAAAATATCCTCTCATCTCGTTCTCTCAGCCGAGTCGGAATCTGCCGCTCTATTCGACATTCAGGCGGAGAAGAAGGAAGCACTGTTTCCGAAGGACTGGGGATTCATAGGCGACGAAGACGAAGAATCGGACGGAGCCGGTGAATCGTTCTGCTTCGATCCGAGGCTGTGCTTGCCTTATTCCGACGAGATTTGTTTACAAGCTTTGCAAGAGCTCGTTCCTGCGATGGAGTATCTCGGACTGAAGATCGTGGAGCACCTATTTCGTGCGATTGGCCTCGAGAATCCAATCGGTGAGGATCGAAGCAGATTCCGGTCGCTGATATGGATATCGAAAGGTTCTCGAAGCGCTGAGCCGGCAGTGACCTGTGGATTTTACCCATACATTGTTGGATTGCATTATCAGAGCAAGAACCAGAGGTGTTCTGTATTAGGAGATTCCGGCTGGGTTGCGACGGCGGCAACGGCGGCGGATTCAGTGATGGTTTCAATCGGAGATATTGCGCAAGTGTGGAGCAATGGAAAGGTAAAGAAAGTGAGAGGCAGGGCAGTAGCGGCGGCGGCTGACGGCAGCGACAACTCGGGCACCATCTCAATATCACTACTCATTACTCTTCCTTTGGATACCCAAGTCTCTCCTCTTCTTCCACCGACCGACGAAAACACAGACGCAGAAGCAGAAGAGGACGGCGGCGGCGGCAACGAGGCAGAGAAGAAAACGGCGGTATTTCGTTCGTTTAGCTTTGAAGAGTACGCGTGGAAAGTGTATCAGAATCGGTGCTTTCTGCTCAGAGATCCGCTGGCCGGATACCGAATCTGAATCGATGGATCGGAGAATTGCCCCATTGTTGATCAATCGACCCATTGAATTCCCACGGTTTTCTGCAGAGGCGTTGAGTACGTAGCTCTACTTTTTCTTTTTTCTTGTTTATTTTGAACAGTTTTTGGATTATTCTTGCGAGTGCTGTGTGATTTTGTTACAAATTTGAATAATCCCAAAAACAATATATTTAGATATTATATGGATAATCAACTTCAGGTTCATTTTTCTTTGAGAAATTTTGTGTTGGGTTTGAATT

Coding sequence (CDS)

ATGGCTTCCTCAGTTGATCACAATAAGTTACCAAACATCTGCGGCGGCGCCACCGCAGCTCCTCCGCCCACTCCTTCCTCACAAACCAACCACCTCTCCACCTCTGCCGCTGCCGAAGCTCTCTCCAGGCTCCTCCACCGCCTCCCCCCCAAACTCTCTCTTCCTACGCGCCGTTCCTCGTCCGTTATATCGCCACCAACGATTTCCTTCTCAGATTCTCCCGATGACGAGTTTTCCAGTAGGCTACTCTCCGCCGTCTCAGAATTCGGCTTCTTCCAAATCACTGATCATAAAATATCCTCTCATCTCGTTCTCTCAGCCGAGTCGGAATCTGCCGCTCTATTCGACATTCAGGCGGAGAAGAAGGAAGCACTGTTTCCGAAGGACTGGGGATTCATAGGCGACGAAGACGAAGAATCGGACGGAGCCGGTGAATCGTTCTGCTTCGATCCGAGGCTGTGCTTGCCTTATTCCGACGAGATTTGTTTACAAGCTTTGCAAGAGCTCGTTCCTGCGATGGAGTATCTCGGACTGAAGATCGTGGAGCACCTATTTCGTGCGATTGGCCTCGAGAATCCAATCGGTGAGGATCGAAGCAGATTCCGGTCGCTGATATGGATATCGAAAGGTTCTCGAAGCGCTGAGCCGGCAGTGACCTGTGGATTTTACCCATACATTGTTGGATTGCATTATCAGAGCAAGAACCAGAGGTGTTCTGTATTAGGAGATTCCGGCTGGGTTGCGACGGCGGCAACGGCGGCGGATTCAGTGATGGTTTCAATCGGAGATATTGCGCAAGTGTGGAGCAATGGAAAGGTAAAGAAAGTGAGAGGCAGGGCAGTAGCGGCGGCGGCTGACGGCAGCGACAACTCGGGCACCATCTCAATATCACTACTCATTACTCTTCCTTTGGATACCCAAGTCTCTCCTCTTCTTCCACCGACCGACGAAAACACAGACGCAGAAGCAGAAGAGGACGGCGGCGGCGGCAACGAGGCAGAGAAGAAAACGGCGGTATTTCGTTCGTTTAGCTTTGAAGAGTACGCGTGGAAAGTGTATCAGAATCGGTGCTTTCTGCTCAGAGATCCGCTGGCCGGATACCGAATCTGA

Protein sequence

MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSSSVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAEKKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKIVEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLITLPLDTQVSPLLPPTDENTDAEAEEDGGGGNEAEKKTAVFRSFSFEEYAWKVYQNRCFLLRDPLAGYRI

Homology

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BLAST of Carg18375 vs. NCBI nr
Match: XP_022943276.1 (gibberellin 3-beta-dioxygenase 4 [Cucurbita moschata])

HSP 1 Score: 706.4 bits (1822), Expect = 4.9e-200
Identity = 369/369 (100.00%), Postives = 369/369 (100.00%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60
           MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS
Sbjct: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60

Query: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120
           SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE
Sbjct: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120

Query: 121 KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI 180
           KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI
Sbjct: 121 KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI 180

Query: 181 VEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV 240
           VEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV
Sbjct: 181 VEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV 240

Query: 241 LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI 300
           LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI
Sbjct: 241 LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI 300

Query: 301 TLPLDTQVSPLLPPTDENTDAEAXXXXXXXXXAEKKTAVFRSFSFEEYAWKVYQNRCFLL 360
           TLPLDTQVSPLLPPTDENTDAEAXXXXXXXXXAEKKTAVFRSFSFEEYAWKVYQNRCFLL
Sbjct: 301 TLPLDTQVSPLLPPTDENTDAEAXXXXXXXXXAEKKTAVFRSFSFEEYAWKVYQNRCFLL 360

Query: 361 RDPLAGYRI 370
           RDPLAGYRI
Sbjct: 361 RDPLAGYRI 369

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BLAST of Carg18375 vs. NCBI nr
Match: XP_023524652.1 (gibberellin 3-beta-dioxygenase 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 693.3 bits (1788), Expect = 4.3e-196
Identity = 356/369 (96.48%), Postives = 358/369 (97.02%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60
           MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS
Sbjct: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60

Query: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120
           SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHL+LSAESESAALFDIQAE
Sbjct: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLLLSAESESAALFDIQAE 120

Query: 121 KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI 180
           KKEALFPKDWGFIGDEDEESDGA ESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI
Sbjct: 121 KKEALFPKDWGFIGDEDEESDGACESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI 180

Query: 181 VEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV 240
           +E LFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV
Sbjct: 181 IEPLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV 240

Query: 241 LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI 300
           LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI
Sbjct: 241 LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI 300

Query: 301 TLPLDTQVSPLLPPTDENTDAEAXXXXXXXXXAEKKTAVFRSFSFEEYAWKVYQNRCFLL 360
           TLPLDTQVSPLLPPTDENTDAEA         AEKKTAVFRSFSFEEYAWKVYQNRCFLL
Sbjct: 301 TLPLDTQVSPLLPPTDENTDAEA-KEDGGGNEAEKKTAVFRSFSFEEYAWKVYQNRCFLL 360

Query: 361 RDPLAGYRI 370
           RDPLAGYRI
Sbjct: 361 RDPLAGYRI 368

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BLAST of Carg18375 vs. NCBI nr
Match: XP_022988289.1 (uncharacterized protein LOC111485581 [Cucurbita maxima])

HSP 1 Score: 688.0 bits (1774), Expect = 1.8e-194
Identity = 354/369 (95.93%), Postives = 355/369 (96.21%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60
           MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS
Sbjct: 103 MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 162

Query: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120
           SVISPPTISFS+SPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSA SESAALFDIQAE
Sbjct: 163 SVISPPTISFSESPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAVSESAALFDIQAE 222

Query: 121 KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI 180
           KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI
Sbjct: 223 KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI 282

Query: 181 VEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV 240
           VE LFRAIGLENPIGE RSRFRSLIW SKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV
Sbjct: 283 VEPLFRAIGLENPIGEGRSRFRSLIWTSKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV 342

Query: 241 LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI 300
           LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI
Sbjct: 343 LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI 402

Query: 301 TLPLDTQVSPLLPPTDENTDAEAXXXXXXXXXAEKKTAVFRSFSFEEYAWKVYQNRCFLL 360
           TLPLDTQVSPLLPPTDENTDAEA          EKKTAVFRSFSFEEYAWKVYQNRCFLL
Sbjct: 403 TLPLDTQVSPLLPPTDENTDAEA-EEDGGGNEVEKKTAVFRSFSFEEYAWKVYQNRCFLL 462

Query: 361 RDPLAGYRI 370
           RDPLAGYRI
Sbjct: 463 RDPLAGYRI 470

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BLAST of Carg18375 vs. NCBI nr
Match: XP_008465218.1 (PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase [Cucumis melo])

HSP 1 Score: 494.2 bits (1271), Expect = 3.9e-136
Identity = 267/383 (69.71%), Postives = 303/383 (79.11%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60
           MASS++HN+LPNI GGATAAPPPTPSSQTNHLSTSAAA+ALS+LLHRLPP LSLPTRRS 
Sbjct: 1   MASSLNHNQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSKLLHRLPPNLSLPTRRSP 60

Query: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120
           SVI+PPTISFS+SP+ +  + LLSA SE GFFQ+TDHKISSHL LSAESESAALF++ AE
Sbjct: 61  SVIAPPTISFSESPNPDLLNHLLSAASELGFFQLTDHKISSHLALSAESESAALFNLSAE 120

Query: 121 KKEALFPKDW--GFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGL 180
           KKE+LFPK+W  GF GD DEESDG+G+S CFD RLC   S EI L +L E V  ME LGL
Sbjct: 121 KKESLFPKNWPLGFKGDGDEESDGSGDSLCFDSRLCFSDSAEISLHSLTEFVLEMESLGL 180

Query: 181 KIVEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRC 240
           KIVE LFRAIG ENPIGEDR+ FRSL+WIS+G R  EPA+  GFYPYIVGL YQS+NQRC
Sbjct: 181 KIVEFLFRAIGFENPIGEDRTGFRSLVWISEGCRGTEPAMAGGFYPYIVGLQYQSRNQRC 240

Query: 241 SVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAAD----GSDNSGTI 300
           S+LGDSGWVA AA AADSVMVSIGD+AQVWSNGK+KK+RGR V  A+      S NS TI
Sbjct: 241 SLLGDSGWVA-AAAAADSVMVSIGDVAQVWSNGKLKKMRGRPVPMASSLANTSSTNSRTI 300

Query: 301 SISLLITLPLDTQVSPLLPPTDENTDAEAXXXXXXXXXAE--------KKTAVFRSFSFE 360
           S+SLLITLP+DTQVSPLL  T+E    E           E        K+ AVF SF+FE
Sbjct: 301 SLSLLITLPVDTQVSPLL-STNEXXXXEQFASVRDKMKKEEDADNDEGKEKAVFHSFNFE 360

Query: 361 EYAWKVYQNRCFLLRDPLAGYRI 370
           EYAW+VY +RCFLL+DPL  YRI
Sbjct: 361 EYAWRVYPDRCFLLKDPLNRYRI 381

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BLAST of Carg18375 vs. NCBI nr
Match: XP_011654997.1 (PREDICTED: gibberellin 2-beta-dioxygenase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 446.8 bits (1148), Expect = 7.1e-122
Identity = 251/379 (66.23%), Postives = 285/379 (75.20%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60
           MASS+DHN+LPNI  GATAAPPPTPSSQTNHLSTSAAA+ALS+LLHRLPP LSLPTRRS 
Sbjct: 1   MASSLDHNQLPNIHAGATAAPPPTPSSQTNHLSTSAAADALSKLLHRLPPNLSLPTRRSP 60

Query: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120
           SVI PPTISFS+SP+ +  +RLLSA SE GFFQ+TDHKISSHL LSAESESA LF++ AE
Sbjct: 61  SVIPPPTISFSESPNPDLLNRLLSAASELGFFQLTDHKISSHLALSAESESAPLFNLPAE 120

Query: 121 KKEALFPKDW--GFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGL 180
           KKE+LFPK+W  GF GD DEESDG+GES CFD R CL  S EI   +L + V  ME LGL
Sbjct: 121 KKESLFPKNWPLGFKGDGDEESDGSGESLCFDSRNCLSDSPEISFHSLTDFVLEMESLGL 180

Query: 181 KIVEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRC 240
           KIVE LFRAIG ENPIGEDR+ FRSL+WIS+G RS EPA+  GFYPYI+GL YQS+NQ+C
Sbjct: 181 KIVEFLFRAIGFENPIGEDRTGFRSLVWISEGCRSTEPAMAGGFYPYIIGLQYQSRNQKC 240

Query: 241 SVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGT----- 300
           S+LGDSGWVA AA AADSVMVSIGDIAQVWSNGK+KK+RGR V  A+  ++ S T     
Sbjct: 241 SLLGDSGWVA-AAAAADSVMVSIGDIAQVWSNGKLKKMRGRPVPMASSVANTSSTNXXXX 300

Query: 301 ---ISISLLITLPLDTQVSPLLPPTDENTDAEAXXXXXXXXXAEKKTAVFRSFSFEEYAW 360
                                            XXXXXXXXX   + AVF SF+FEEYAW
Sbjct: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKAVFHSFNFEEYAW 360

Query: 361 KVYQNRCFLLRDPLAGYRI 370
           +VY +RCFLL+DPL  YRI
Sbjct: 361 RVYHDRCFLLKDPLNRYRI 378

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BLAST of Carg18375 vs. TAIR10
Match: AT3G11150.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 233.4 bits (594), Expect = 2.2e-61
Identity = 161/365 (44.11%), Postives = 221/365 (60.55%), Query Frame = 0


Query: 15  GGATAAPPPTPSSQTNHLSTSA----AAEALSRLLHRLPPKLSLPTRRSSSVISPPTISF 74
           GGATAAPPPTPS   +HL+T++    A +ALS L HRLPP LSLP RR  S+ S P +S 
Sbjct: 18  GGATAAPPPTPS--CSHLNTTSKSETAVDALSSLFHRLPPLLSLPNRR--SIPSLPMVSL 77

Query: 75  SDSPDDEFSSRLLSAVSEFGFFQI----TDHKISSHLVLSAESESAALFDIQAEKKEALF 134
           S     E+   L+SAV++FG+FQ+    +D      +  +AES+S +L ++  EKKE+ F
Sbjct: 78  SAGDRLEWDD-LISAVTDFGYFQLINDDSDILFPPGIAEAAESDSLSLLELSEEKKESSF 137

Query: 135 PKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKIVEHLFR 194
           PK W        E+D    SFC D   C   S E+ L +L+E    +E +GLK VE L  
Sbjct: 138 PKKWPL----GYEADAETPSFCLDAD-CSTDSSELNLSSLREFTRTLEKVGLKTVEMLAN 197

Query: 195 AIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSVLGDSGW 254
           A+G     G D +RF +L+W+++G    EP VT GFYP++V L YQ + Q+  +L +SGW
Sbjct: 198 ALG----FGYDSTRFNTLMWVNQGVPDDEPEVTNGFYPFVVCLQYQIREQKYCLLTESGW 257

Query: 255 VATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLITLPLDT 314
           V +     DSV+V++GDIAQVW NG+VK+V+ R V  +         ++++L++TLP+D+
Sbjct: 258 V-SVLPRVDSVLVTLGDIAQVWRNGEVKRVKYRPVLCSGQKDGPVKCVTMTLMLTLPMDS 317

Query: 315 QVSPL--LPPTDENTDAEAXXXXXXXXXAEKKTAVFRSFSFEEYAWKVYQNRCFLLRDPL 370
            VS L  +    +      XXXXXXXXX       FR FSF+EYAW+VYQ R F  RDPL
Sbjct: 318 MVSSLKDMISDGDKXXXXXXXXXXXXXXXXXXXRAFRPFSFKEYAWRVYQERLF-FRDPL 366

[Close]

BLAST of Carg18375 vs. TAIR10
Match: AT2G34555.1 (gibberellin 2-oxidase 3)

HSP 1 Score: 52.8 bits (125), Expect = 5.4e-07
Identity = 59/271 (21.77%), Postives = 107/271 (39.48%), Query Frame = 0


Query: 65  PPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAEKKEA 124
           P  I   D  D +  ++++ A  EFGFF++ +H +   L+   E E+   F +    K+ 
Sbjct: 24  PVLIPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDK 83

Query: 125 LFPKD-WGF----IGDEDEESDGAGESFCFDPRLCLP---------YSDEICLQALQELV 184
             P D +G+    IG   +   G  E    +  LCL          ++  I  +A++E +
Sbjct: 84  AGPPDPFGYGTKRIGPNGDL--GWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYI 143

Query: 185 PAMEYLGLKIVEHLFRAIGLEN--------PIGEDRSRFRSLIWISKGSRSAEPAVTCGF 244
             M+ +  K +E +   + +E          + E  S  R   +  K     +  +  G 
Sbjct: 144 KEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYPEKEETPVKEEIGFGE 203

Query: 245 Y--PYIVGLHYQSKNQRCSVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRA 304
           +  P ++ L   +  +   +    G          S  V +GD  QV +NG+ K V+ R 
Sbjct: 204 HTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRV 263

Query: 305 VAAAADGSDNSGTISISLLITLPLDTQVSPL 312
           V      +     IS+      PL  +++PL
Sbjct: 264 VT-----NTKRSRISMIYFAGPPLSEKIAPL 287

[Close]

BLAST of Carg18375 vs. TAIR10
Match: AT1G80330.1 (gibberellin 3-oxidase 4)

HSP 1 Score: 52.0 bits (123), Expect = 9.2e-07
Identity = 74/294 (25.17%), Postives = 119/294 (40.48%), Query Frame = 0


Query: 58  RSSSVIS----PPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAA 117
           R SS +S      +I   D  + + ++ +  A   +G FQI +H IS  L+   ES S  
Sbjct: 35  RPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKT 94

Query: 118 LFDIQAEKKEALFPKDWGFIG----------DEDEESDG---AGESFCFDPRLCLPYSDE 177
           LFD+ +E+K      D G  G          ++   S+G   A +S+        P+   
Sbjct: 95  LFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGFTIADDSYRNHFNTLWPHDHT 154

Query: 178 ICLQALQELVPAMEYLGLKIVEHLFRAIGLENPIGEDRSRFRSLIWISK----GSRSAEP 237
                +QE V  ME L  +++  +  ++G+             + W  K    GS+    
Sbjct: 155 KYCGIIQEYVDEMEKLASRLLYCILGSLGV---------TVEDIEWAHKLEKSGSKVGRG 214

Query: 238 AVTCGFYPY-----------------IVGLHYQSKNQRCSVL-GDSGWVATAATAADSVM 297
           A+    YP                  I+ + +QS      V   +SGWV T   A   ++
Sbjct: 215 AIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWV-TVEPAPGVLV 274

Query: 298 VSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLITLPL-DTQVSPL 312
           V+IGD+  + SNGK+  V  RA       +     ISI+ L   P  D Q++P+
Sbjct: 275 VNIGDLFHILSNGKIPSVVHRAKV-----NHTRSRISIAYLWGGPAGDVQIAPI 313

[Close]

BLAST of Carg18375 vs. TAIR10
Match: AT1G30040.1 (gibberellin 2-oxidase)

HSP 1 Score: 52.0 bits (123), Expect = 9.2e-07
Identity = 71/340 (20.88%), Postives = 131/340 (38.53%), Query Frame = 0


Query: 54  LPTRRSSSVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAA 113
           +PT +   V++  +I   +  D E  +R++ A  EFGFF++ +H +   L+   E E+  
Sbjct: 17  IPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIG 76

Query: 114 LFDI-QAEKKEALFPKDWGF----IGDEDEESDGAGESFCFDPRLCLPYSDEICLQA--- 173
            F + Q+ K  A  P+ +G+    IG   +           +P+L  P +  +  Q    
Sbjct: 77  FFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPXX 136

Query: 174 ----LQELVPAMEYLGLKIVEHLFRAIGLE------NPIGEDRS-RFRSLIWISKGSRSA 233
                            K++E +   +G+E        + +++S     L         A
Sbjct: 137 XXXXXXXXXXXXXXXSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEEEA 196

Query: 234 EPAVTCGF----YPYIVGLHYQSKNQRCSV-LGDSGWVATAATAADSVMVSIGDIAQVWS 293
           E  V  GF     P I+ +   +      + + D  WVA     + S  +++GD  QV +
Sbjct: 197 EKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHS-SFFINVGDALQVMT 256

Query: 294 NGKVKKVRGRAVAAAADGSDNSGTISISLLITLPLDTQVSPLLPPTDENTDAEAXXXXXX 353
           NG+ K V+ R +A           IS+      PL  +++PL     E  D         
Sbjct: 257 NGRFKSVKHRVLA-----DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDD--------- 316

Query: 354 XXXAEKKTAVFRSFSFEEYAWKVYQNRCFLLRDPLAGYRI 370
                        + ++E+ W  Y++  +  +  L  YR+
Sbjct: 317 -------------WLYKEFTWSQYKSSAY--KSKLGDYRL 326

[Close]

BLAST of Carg18375 vs. TAIR10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 50.4 bits (119), Expect = 2.7e-06
Identity = 66/282 (23.40%), Postives = 122/282 (43.26%), Query Frame = 0


Query: 62  VISPPTISFSDSPDDEFS-SRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 121
           VI    +S  D+ D  F   +L  A  ++GFFQ+ +H I   +V   E  ++  FD+  E
Sbjct: 57  VIDLSKLSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMPLE 116

Query: 122 KKEALFPKDWG--------FIGDEDEESD-------GAGESFCFDPRLCLPYSDEICLQA 181
           +K+  +P + G        FI  ED++ D       G       +P+L  P       ++
Sbjct: 117 EKKK-YPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKL-WPSKPARFSES 176

Query: 182 LQELVPAMEYLGLKIVEHLFRAIGL-ENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYP 241
           L+     +  L  ++++++  ++GL E    E        + ++     + P +  G  P
Sbjct: 177 LEGYSKEIRELCKRLLKYIAISLGLKEERFEEMFGEAVQAVRMNYYPPCSSPDLVLGLSP 236

Query: 242 YIVG----LHYQSKNQ--RCSVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRG 301
           +  G    +  QSKN      +L D+ WV       ++++++IGD  +V SNGK K V  
Sbjct: 237 HSDGSALTVLQQSKNSCVGLQILKDNTWV-PVKPLPNALVINIGDTIEVLSNGKYKSVEH 296

Query: 302 RAVAAAADGSDNSGTISISLLITLPLDTQVSPLLPPTDENTD 321
           RAV      +     ++I        + ++ P+    D+ T+
Sbjct: 297 RAVT-----NREKERLTIVTFYAPNYEVEIEPMSELVDDETN 330

[Close]

BLAST of Carg18375 vs. Swiss-Prot
Match: sp|O64692|G2OX3_ARATH (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 9.7e-06
Identity = 59/271 (21.77%), Postives = 107/271 (39.48%), Query Frame = 0


Query: 65  PPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAEKKEA 124
           P  I   D  D +  ++++ A  EFGFF++ +H +   L+   E E+   F +    K+ 
Sbjct: 24  PVLIPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDK 83

Query: 125 LFPKD-WGF----IGDEDEESDGAGESFCFDPRLCLP---------YSDEICLQALQELV 184
             P D +G+    IG   +   G  E    +  LCL          ++  I  +A++E +
Sbjct: 84  AGPPDPFGYGTKRIGPNGDL--GWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYI 143

Query: 185 PAMEYLGLKIVEHLFRAIGLEN--------PIGEDRSRFRSLIWISKGSRSAEPAVTCGF 244
             M+ +  K +E +   + +E          + E  S  R   +  K     +  +  G 
Sbjct: 144 KEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYPEKEETPVKEEIGFGE 203

Query: 245 Y--PYIVGLHYQSKNQRCSVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRA 304
           +  P ++ L   +  +   +    G          S  V +GD  QV +NG+ K V+ R 
Sbjct: 204 HTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRV 263

Query: 305 VAAAADGSDNSGTISISLLITLPLDTQVSPL 312
           V      +     IS+      PL  +++PL
Sbjct: 264 VT-----NTKRSRISMIYFAGPPLSEKIAPL 287

[Close]

BLAST of Carg18375 vs. Swiss-Prot
Match: sp|Q9XFR9|G2OX2_ARATH (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.7e-05
Identity = 71/340 (20.88%), Postives = 131/340 (38.53%), Query Frame = 0


Query: 54  LPTRRSSSVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAA 113
           +PT +   V++  +I   +  D E  +R++ A  EFGFF++ +H +   L+   E E+  
Sbjct: 17  IPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIG 76

Query: 114 LFDI-QAEKKEALFPKDWGF----IGDEDEESDGAGESFCFDPRLCLPYSDEICLQA--- 173
            F + Q+ K  A  P+ +G+    IG   +           +P+L  P +  +  Q    
Sbjct: 77  FFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPXX 136

Query: 174 ----LQELVPAMEYLGLKIVEHLFRAIGLE------NPIGEDRS-RFRSLIWISKGSRSA 233
                            K++E +   +G+E        + +++S     L         A
Sbjct: 137 XXXXXXXXXXXXXXXSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEEEA 196

Query: 234 EPAVTCGF----YPYIVGLHYQSKNQRCSV-LGDSGWVATAATAADSVMVSIGDIAQVWS 293
           E  V  GF     P I+ +   +      + + D  WVA     + S  +++GD  QV +
Sbjct: 197 EKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHS-SFFINVGDALQVMT 256

Query: 294 NGKVKKVRGRAVAAAADGSDNSGTISISLLITLPLDTQVSPLLPPTDENTDAEAXXXXXX 353
           NG+ K V+ R +A           IS+      PL  +++PL     E  D         
Sbjct: 257 NGRFKSVKHRVLA-----DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDD--------- 316

Query: 354 XXXAEKKTAVFRSFSFEEYAWKVYQNRCFLLRDPLAGYRI 370
                        + ++E+ W  Y++  +  +  L  YR+
Sbjct: 317 -------------WLYKEFTWSQYKSSAY--KSKLGDYRL 326

[Close]

BLAST of Carg18375 vs. Swiss-Prot
Match: sp|Q9C971|G3OX4_ARATH (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.7e-05
Identity = 74/294 (25.17%), Postives = 119/294 (40.48%), Query Frame = 0


Query: 58  RSSSVIS----PPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAA 117
           R SS +S      +I   D  + + ++ +  A   +G FQI +H IS  L+   ES S  
Sbjct: 35  RPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKT 94

Query: 118 LFDIQAEKKEALFPKDWGFIG----------DEDEESDG---AGESFCFDPRLCLPYSDE 177
           LFD+ +E+K      D G  G          ++   S+G   A +S+        P+   
Sbjct: 95  LFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGFTIADDSYRNHFNTLWPHDHT 154

Query: 178 ICLQALQELVPAMEYLGLKIVEHLFRAIGLENPIGEDRSRFRSLIWISK----GSRSAEP 237
                +QE V  ME L  +++  +  ++G+             + W  K    GS+    
Sbjct: 155 KYCGIIQEYVDEMEKLASRLLYCILGSLGV---------TVEDIEWAHKLEKSGSKVGRG 214

Query: 238 AVTCGFYPY-----------------IVGLHYQSKNQRCSVL-GDSGWVATAATAADSVM 297
           A+    YP                  I+ + +QS      V   +SGWV T   A   ++
Sbjct: 215 AIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWV-TVEPAPGVLV 274

Query: 298 VSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLITLPL-DTQVSPL 312
           V+IGD+  + SNGK+  V  RA       +     ISI+ L   P  D Q++P+
Sbjct: 275 VNIGDLFHILSNGKIPSVVHRAKV-----NHTRSRISIAYLWGGPAGDVQIAPI 313

[Close]

BLAST of Carg18375 vs. Swiss-Prot
Match: sp|D4N501|DIOX2_PAPSO (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=3469 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 2.4e-04
Identity = 55/263 (20.91%), Postives = 114/263 (43.35%), Query Frame = 0


Query: 81  RLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAEKKEALFPKD-------WGFI 140
           RL SA  E+GFFQ+ +H + + LV + +S+    F++   +K     KD         F+
Sbjct: 77  RLHSACKEWGFFQVVNHGVDTSLVDNVKSDIQGFFNLSMNEKIKYGQKDGDVEGFGQAFV 136

Query: 141 GDEDEESDGAGESFCFDPRLCL--PYSDEICLQALQELVPA----MEYLGLKIVEHLFRA 200
             ED+  D A         L L  P+        L+E + +    M+ L + + E + +A
Sbjct: 137 ASEDQTLDWADIFMILTLPLHLRKPHLFSKLPLPLRETIESYSSEMKKLSMVLFEKMEKA 196

Query: 201 IGLEN-PIGEDRSRFRSLIWISKGS---RSAEPAVTCGFYPY-----IVGLHYQSKNQRC 260
           + ++   I E    F+ +  + + +      +P +  G  P+     +  L   ++ +  
Sbjct: 197 LQVQAVEIKEISEVFKDMTQVMRMNYYPPCPQPELAIGLTPHSDFGGLTILLQLNEVEGL 256

Query: 261 SVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISL 320
            +  +  W+ +     ++ +V++GD+ ++ +NG  + V  RAV      +     +SI+ 
Sbjct: 257 QIKNEGRWI-SVKPLPNAFVVNVGDVLEIMTNGMYRSVDHRAVV-----NSTKERLSIAT 316

Query: 321 LITLPLDTQVSPLLPPTDENTDA 322
                L++++ P+      NT A
Sbjct: 317 FHDPNLESEIGPISSLITPNTPA 333

[Close]

BLAST of Carg18375 vs. Swiss-Prot
Match: sp|P07920|ACCO2_SOLLC (1-aminocyclopropane-1-carboxylate oxidase 2 OS=Solanum lycopersicum OX=4081 GN=ACO2 PE=2 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 9.1e-04
Identity = 70/304 (23.03%), Postives = 123/304 (40.46%), Query Frame = 0


Query: 81  RLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAEKKEALFPKDWGFIGDEDEES 140
           ++  A   +GFF++ +H I   ++ + E  +   +    E++        G  G E E +
Sbjct: 23  KINDACENWGFFELVNHGIPHEVMDTVEKLTKGHYKKCMEQRFKELVAKKGLEGVEVEVT 82

Query: 141 DGAGESFCFDPRL------CLPYSDEICLQALQELVPAMEYLGLKIVEHLFRAIGLENPI 200
           D   ES  F   L       LP  D++  + +++    +E L  ++++ L   +GLE   
Sbjct: 83  DMDWESTFFLRHLPSSNISQLPDLDDVYREVMRDFRKRLEKLAEELLDLLCENLGLE--- 142

Query: 201 GEDRSRFRSLIWISKGSR----------SAEPAVTCGFYPY-----IVGLHYQSKNQRCS 260
              +S  ++  + SKG              +P +  G   +     I+ L    K     
Sbjct: 143 ---KSYLKNTFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDAGGIILLFQDDKVSGLQ 202

Query: 261 VLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLL 320
           +L D  W+        S++V++GD  +V +NGK K V  R V A  DG+     +S++  
Sbjct: 203 LLKDGRWI-DVPPMRHSIVVNLGDQLEVITNGKYKSVMHR-VIAQKDGT----RMSLASF 262

Query: 321 ITLPLDTQVSPLLPPTDENTDAEAXXXXXXXXXAEKKTAVFRSFSFEEYAWKVYQNRCFL 364
                D     L+ P     D EA          E    V+  F F++Y  K+Y N  F 
Sbjct: 263 YNPGNDA----LIYPAPALVDKEA---------EEHNKQVYPKFMFDDY-MKLYANLKFQ 300

[Close]

BLAST of Carg18375 vs. TrEMBL
Match: tr|A0A1S3CNS4|A0A1S3CNS4_CUCME (1-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo OX=3656 GN=LOC103502873 PE=4 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 2.5e-136
Identity = 267/383 (69.71%), Postives = 303/383 (79.11%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60
           MASS++HN+LPNI GGATAAPPPTPSSQTNHLSTSAAA+ALS+LLHRLPP LSLPTRRS 
Sbjct: 1   MASSLNHNQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSKLLHRLPPNLSLPTRRSP 60

Query: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120
           SVI+PPTISFS+SP+ +  + LLSA SE GFFQ+TDHKISSHL LSAESESAALF++ AE
Sbjct: 61  SVIAPPTISFSESPNPDLLNHLLSAASELGFFQLTDHKISSHLALSAESESAALFNLSAE 120

Query: 121 KKEALFPKDW--GFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGL 180
           KKE+LFPK+W  GF GD DEESDG+G+S CFD RLC   S EI L +L E V  ME LGL
Sbjct: 121 KKESLFPKNWPLGFKGDGDEESDGSGDSLCFDSRLCFSDSAEISLHSLTEFVLEMESLGL 180

Query: 181 KIVEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRC 240
           KIVE LFRAIG ENPIGEDR+ FRSL+WIS+G R  EPA+  GFYPYIVGL YQS+NQRC
Sbjct: 181 KIVEFLFRAIGFENPIGEDRTGFRSLVWISEGCRGTEPAMAGGFYPYIVGLQYQSRNQRC 240

Query: 241 SVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAAD----GSDNSGTI 300
           S+LGDSGWVA AA AADSVMVSIGD+AQVWSNGK+KK+RGR V  A+      S NS TI
Sbjct: 241 SLLGDSGWVA-AAAAADSVMVSIGDVAQVWSNGKLKKMRGRPVPMASSLANTSSTNSRTI 300

Query: 301 SISLLITLPLDTQVSPLLPPTDENTDAEAXXXXXXXXXAE--------KKTAVFRSFSFE 360
           S+SLLITLP+DTQVSPLL  T+E    E           E        K+ AVF SF+FE
Sbjct: 301 SLSLLITLPVDTQVSPLL-STNEXXXXEQFASVRDKMKKEEDADNDEGKEKAVFHSFNFE 360

Query: 361 EYAWKVYQNRCFLLRDPLAGYRI 370
           EYAW+VY +RCFLL+DPL  YRI
Sbjct: 361 EYAWRVYPDRCFLLKDPLNRYRI 381

[Close]

BLAST of Carg18375 vs. TrEMBL
Match: tr|A0A0A0KP52|A0A0A0KP52_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G208470 PE=4 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 3.0e-105
Identity = 203/269 (75.46%), Postives = 228/269 (84.76%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60
           MASS+DHN+LPNI  GATAAPPPTPSSQTNHLSTSAAA+ALS+LLHRLPP LSLPTRRS 
Sbjct: 1   MASSLDHNQLPNIHAGATAAPPPTPSSQTNHLSTSAAADALSKLLHRLPPNLSLPTRRSP 60

Query: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120
           SVI PPTISFS+SP+ +  +RLLSA SE GFFQ+TDHKISSHL LSAESESA LF++ AE
Sbjct: 61  SVIPPPTISFSESPNPDLLNRLLSAASELGFFQLTDHKISSHLALSAESESAPLFNLPAE 120

Query: 121 KKEALFPKDW--GFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGL 180
           KKE+LFPK+W  GF GD DEESDG+GES CFD R CL  S EI   +L + V  ME LGL
Sbjct: 121 KKESLFPKNWPLGFKGDGDEESDGSGESLCFDSRNCLSDSPEISFHSLTDFVLEMESLGL 180

Query: 181 KIVEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRC 240
           KIVE LFRAIG ENPIGEDR+ FRSL+WIS+G RS EPA+  GFYPYI+GL YQS+NQ+C
Sbjct: 181 KIVEFLFRAIGFENPIGEDRTGFRSLVWISEGCRSTEPAMAGGFYPYIIGLQYQSRNQKC 240

Query: 241 SVLGDSGWVATAATAADSVMVSIGDIAQV 268
           S+LGDSGWVA AA AADSVMVSIGDIAQ+
Sbjct: 241 SLLGDSGWVA-AAAAADSVMVSIGDIAQL 268

[Close]

BLAST of Carg18375 vs. TrEMBL
Match: tr|A0A061FKB2|A0A061FKB2_THECC (2-oxoglutarate and Fe(II)-dependent oxygenase domain-containing protein, putative OS=Theobroma cacao OX=3641 GN=TCM_042485 PE=4 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 1.2e-88
Identity = 194/379 (51.19%), Postives = 255/379 (67.28%), Query Frame = 0


Query: 1   MASSV--DHNKLPNICGGATAAPPPTPSSQTNH--LSTSAAAEALSRLLHRLPPKLSLPT 60
           MASS       LPN+  GATAAPPPTPS Q NH  +++SA A+ALS+LLHRLPP LSLPT
Sbjct: 1   MASSTHKQQQHLPNLY-GATAAPPPTPSGQPNHHLVTSSATADALSKLLHRLPPTLSLPT 60

Query: 61  RRSS-SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALF 120
            RS+ S  SP T+SFS   D      LLS+ S+ GFFQ+T H +SS L  SAE+ES +LF
Sbjct: 61  HRSAPSTASPRTVSFS---DPNLKDLLLSSGSKVGFFQLTSHDVSSQLANSAETESLSLF 120

Query: 121 DIQAEKKEALFPKDW--GFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAM 180
           ++  E+KE+ FPK+W  GF  DE+E  D  GESFC D   C      + L +L+E   A+
Sbjct: 121 ELPKEQKESCFPKNWPLGFDADEEEGGDEKGESFCLD-ATCSTELTNLSLSSLREFTRAL 180

Query: 181 EYLGLKIVEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQS 240
           E LGLKI++ L  A+G ENPIGED +RF SL+WI +G    +   + GFYP+++GL YQ 
Sbjct: 181 EKLGLKIIDTLANAVGFENPIGEDPTRFCSLMWILEGLH-GDDKPSGGFYPFVIGLQYQI 240

Query: 241 KNQRCSVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGT 300
           + Q+ S+L +SGWV + +   DS+MV++GDIAQVWSNGK++KVRGR VAA  D  +NS  
Sbjct: 241 RCQQYSLLSESGWV-SVSPEVDSIMVTLGDIAQVWSNGKLRKVRGRPVAACLDDGNNSRH 300

Query: 301 ISISLLITLPLDTQVSPLLPPT---DENTDAEAXXXXXXXXXAEKKTAVFRSFSFEEYAW 360
           +S+SLL+TLP+D+QV+PLL      DEN   +           +K+  +FRSFSFE+YAW
Sbjct: 301 VSMSLLLTLPMDSQVAPLLTKVIADDENASDDEIRDDEIGTEGKKEGRLFRSFSFEDYAW 360

Query: 361 KVYQNRCFLLRDPLAGYRI 370
           +VY + C L +DPL  YRI
Sbjct: 361 RVY-HECLLFKDPLDRYRI 371

[Close]

BLAST of Carg18375 vs. TrEMBL
Match: tr|A0A2R6RWR1|A0A2R6RWR1_ACTCH (Gibberellin 2-beta-dioxygenase OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc01566 PE=4 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 7.8e-85
Identity = 187/376 (49.73%), Postives = 244/376 (64.89%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRR-- 60
           MASS  H+ L N  GG+TAAPPPTPS+Q N L+ S  A+ LS LLHRLPP LSLPTRR  
Sbjct: 1   MASST-HHHLSNPYGGSTAAPPPTPSTQPNPLA-SPDADVLSLLLHRLPPALSLPTRRSP 60

Query: 61  -SSSVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDI 120
            S+   SPP +S S    +  +  LLSA S+ GFFQ+T H + S L  SAESES +LF++
Sbjct: 61  PSTPTTSPPLVSLSPQNPNSLAD-LLSAASQLGFFQLTTHSVPSQLAQSAESESLSLFNL 120

Query: 121 QAEKKEALFPKDW--GFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEY 180
             E+K+  FP +W  GF  D++E+ +G   SFC D   C   S E+ L +L EL   ME 
Sbjct: 121 SKEQKQLHFPPNWPLGFDCDDEEDENGTNASFCIDSS-CSTESTELSLSSLCELTREMEK 180

Query: 181 LGLKIVEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKN 240
           LGL ++E L  A+G ENP+ ED +R  SL+W+S+G    EP ++  FYPY+VGLHYQ + 
Sbjct: 181 LGLDVIEALSCAVGFENPVREDPTRVCSLMWVSEGCTGKEPVMSGKFYPYVVGLHYQIRR 240

Query: 241 QRCSVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTIS 300
           QRCS+L DSGWV + +   DSV+V++GD+AQVWSNGK KKVRGR V +  D + N+  I 
Sbjct: 241 QRCSLLSDSGWV-SVSPRVDSVLVTLGDLAQVWSNGKTKKVRGRPVPSFDDSTKNARCIW 300

Query: 301 ISLLITLPLDTQVSPLLP--PTDENTDAEAXXXXXXXXXAEKKTAVFRSFSFEEYAWKVY 360
           +SLL+TLPL++ VSPL+P        D +A            ++ VF SFSFE+YAW+VY
Sbjct: 301 MSLLVTLPLESTVSPLIPMVVATNGEDDQAKKEDGGSSEDTMESRVFNSFSFEDYAWRVY 360

Query: 361 QNRCFLLRDPLAGYRI 370
             R  L +DPL  YRI
Sbjct: 361 HER-LLYKDPLDRYRI 370

[Close]

BLAST of Carg18375 vs. TrEMBL
Match: tr|A0A2C9VN29|A0A2C9VN29_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_07G130900 PE=4 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 5.0e-84
Identity = 202/380 (53.16%), Postives = 263/380 (69.21%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQ--TNHLSTSAAAEALSRLLHRLPPKLSLPTRR 60
           MASS     LPN    A +APPPTPSSQ  T+H++++A A+ALSRLLHRLPP LSLPTRR
Sbjct: 1   MASSAQKQHLPNFY-TAASAPPPTPSSQPNTHHVTSAADADALSRLLHRLPPILSLPTRR 60

Query: 61  SSSVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQ 120
           SS   SPP IS S   D   S  LLSA S+ GFFQ+TDH I S L  S+ESE+ +LF++ 
Sbjct: 61  SSPATSPPVISLS---DPNSSDLLLSASSQHGFFQLTDHDIPSQLAHSSESEARSLFELT 120

Query: 121 AEKKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGL 180
            +KKE+ FPK+W  +G ED+E DG GESF  D   C   S E+ L +L+EL  AME +G+
Sbjct: 121 RDKKESYFPKNWP-LGFEDDE-DGNGESFWLD-AACSTESTELNLSSLRELTQAMEKVGM 180

Query: 181 KIVEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRC 240
           +I+++L R +G ENP+ ED +R  SL+ + + S   +PA++ GFYPYIVGL YQ ++Q+ 
Sbjct: 181 EIIDNLAREVGFENPLKEDLTRNCSLMLVHESSCEDKPAMSGGFYPYIVGLQYQIRSQKY 240

Query: 241 SVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISL 300
           S+L DSGWVA      DSVMV++GDIAQVWSNGK+KKVRGR  A+  + S +S  I+++L
Sbjct: 241 SLLSDSGWVA-VEPRMDSVMVTVGDIAQVWSNGKLKKVRGRPTASMGE-SKSSSCITMTL 300

Query: 301 LITLPLDTQVSPLLPPTDENT---------DAEAXXXXXXXXXAEKKTAVFRSFSFEEYA 360
           LITLP +++VSPLLP  +EN              XXXXXXXXX  ++  +FRSFSFE+YA
Sbjct: 301 LITLPTESKVSPLLPINEENAXXXXXXXXXXXXXXXXXXXXXXXXEERLMFRSFSFEDYA 360

Query: 361 WKVYQNRCFLLRDPLAGYRI 370
           W+VY +   L +DPL  YRI
Sbjct: 361 WRVY-HEPLLFKDPLHKYRI 370

The following BLAST results are available for this feature:

BLAST of Carg18375 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022943276.1	4.9e-200	100.00	gibberellin 3-beta-dioxygenase 4 [Cucurbita moschata]	[more]
XP_023524652.1	4.3e-196	96.48	gibberellin 3-beta-dioxygenase 4 [Cucurbita pepo subsp. pepo]	[more]
XP_022988289.1	1.8e-194	95.93	uncharacterized protein LOC111485581 [Cucurbita maxima]	[more]
XP_008465218.1	3.9e-136	69.71	PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase [Cucumis melo]	[more]
XP_011654997.1	7.1e-122	66.23	PREDICTED: gibberellin 2-beta-dioxygenase 2 isoform X1 [Cucumis sativus]	[more]

BLAST of Carg18375 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G11150.1	2.2e-61	44.11	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G34555.1	5.4e-07	21.77	gibberellin 2-oxidase 3	[more]
AT1G80330.1	9.2e-07	25.17	gibberellin 3-oxidase 4	[more]
AT1G30040.1	9.2e-07	20.88	gibberellin 2-oxidase	[more]
AT3G21420.1	2.7e-06	23.40	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

BLAST of Carg18375 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O64692\|G2OX3_ARATH	9.7e-06	21.77	Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 ...	[more]
sp\|Q9XFR9\|G2OX2_ARATH	1.7e-05	20.88	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
sp\|Q9C971\|G3OX4_ARATH	1.7e-05	25.17	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ...	[more]
sp\|D4N501\|DIOX2_PAPSO	2.4e-04	20.91	Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=34...	[more]
sp\|P07920\|ACCO2_SOLLC	9.1e-04	23.03	1-aminocyclopropane-1-carboxylate oxidase 2 OS=Solanum lycopersicum OX=4081 GN=A...	[more]

BLAST of Carg18375 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3CNS4\|A0A1S3CNS4_CUCME	2.5e-136	69.71	1-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo OX=3656 GN=LOC10350287...	[more]
tr\|A0A0A0KP52\|A0A0A0KP52_CUCSA	3.0e-105	75.46	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G208470 PE=4 SV=1	[more]
tr\|A0A061FKB2\|A0A061FKB2_THECC	1.2e-88	51.19	2-oxoglutarate and Fe(II)-dependent oxygenase domain-containing protein, putativ...	[more]
tr\|A0A2R6RWR1\|A0A2R6RWR1_ACTCH	7.8e-85	49.73	Gibberellin 2-beta-dioxygenase OS=Actinidia chinensis var. chinensis OX=1590841 ...	[more]
tr\|A0A2C9VN29\|A0A2C9VN29_MANES	5.0e-84	53.16	Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_07G130900 PE=4 SV=...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR026992	DIOX_N
IPR027443	IPNS-like

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18375-RA	Carg18375-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 226..361 e-value: 2.3E-7 score: 32.4 coord: 64..224 e-value: 2.8E-10 score: 42.0
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 66..128 e-value: 1.9E-8 score: 35.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 311..333
None	No IPR available	PANTHER	PTHR34945:SF1	2-OXOGLUTARATE 2OG AND FE II -DEPENDENT OXYGENASE SUPERFAMILY PROTEIN	coord: 1..369
None	No IPR available	PANTHER	PTHR34945	FAMILY NOT NAMED	coord: 1..369
None	No IPR available	SUPERFAMILY	SSF51197	Clavaminate synthase-like	coord: 49..354

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg18375	Cla004358	Watermelon (97103) v1	carwmB0086
Carg18375	Csa5G208470	Cucumber (Chinese Long) v2	carcuB0098
Carg18375	MELO3C026792	Melon (DHL92) v3.5.1	carmeB0090
Carg18375	ClCG07G004310	Watermelon (Charleston Gray)	carwcgB0090
Carg18375	CSPI05G10780	Wild cucumber (PI 183967)	carcpiB0102
Carg18375	Cucsa.368460	Cucumber (Gy14) v1	carcgyB0109
Carg18375	CmaCh04G001480	Cucurbita maxima (Rimu)	carcmaB0115
Carg18375	CmoCh04G001450	Cucurbita moschata (Rifu)	carcmoB0123
Carg18375	Lsi04G010940	Bottle gourd (USVL1VR-Ls)	carlsiB081
Carg18375	Cp4.1LG01g05290	Cucurbita pepo (Zucchini)	carcpeB0130
Carg18375	CsGy5G008110	Cucumber (Gy14) v2	carcgybB0098
Carg18375	MELO3C026792.2	Melon (DHL92) v3.6.1	carmedB0091
Carg18375	CsaV3_5G010250	Cucumber (Chinese Long) v3	carcucB0105
Carg18375	Cla97C07G132770	Watermelon (97103) v2	carwmbB0091
Carg18375	Bhi07G000495	Wax gourd	carwgoB0103

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg18375	Silver-seed gourd	carcarB097
Carg18375	Silver-seed gourd	carcarB099
Carg18375	Cucurbita maxima (Rimu)	carcmaB0116
Carg18375	Cucurbita moschata (Rifu)	carcmoB0121
Carg18375	Cucurbita moschata (Rifu)	carcmoB0124
Carg18375	Cucurbita pepo (Zucchini)	carcpeB0131
Carg18375	Cucurbita pepo (Zucchini)	carcpeB0132
Carg18375	Wax gourd	carwgoB0102