Carg17810 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg17810
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	polygalacturonase
Location	Cucurbita_argyrosperma_scaffold_070 : 226752 .. 227261 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAAATTTTCCAACCATTCATCACTTCCCTCGCCATTTCAACCATTTATCGTCATACTCGTCATCGTCTTCTTCCTTTTCCTTACGATCACAACGTCATACTCTGCGACTTTATTGAACATTTTGGATTTCGGTGCCATACGCGGCGGGGGACGGGATTCGAGCCCGGCCTTTCGCCGTGCATGGGCAAAGGCTTGCATGTCATCGGAATCCACCATTGTTTACGTTCCAAACGGGAGGTTTTTGGTTCGGCCGATCGAGTTCCACGGCGGGTGTGGCAACAAGGATATTAGTTTCCATATCGACGGGGCTCTGGTTGCACCGTCCGATTATCGTATCCTTGGCGACCTCAGAAGCTGGCTGAGCTTTGATGGCGTTGATGGCGTTACTTTGAGTGGTGGAGTTCTTGATGCCAACGGCGCCGCCTTGTGGGACTGCAAATCCTCCGCCGCCCACTGCCCCGTCGGAGCCATGGTGCCTACCTCTAATTCTAGTTCTATTATTTAA

mRNA sequence

ATGGCCAAATTTTCCAACCATTCATCACTTCCCTCGCCATTTCAACCATTTATCGTCATACTCGTCATCGTCTTCTTCCTTTTCCTTACGATCACAACGTCATACTCTGCGACTTTATTGAACATTTTGGATTTCGGTGCCATACGCGGCGGGGGACGGGATTCGAGCCCGGCCTTTCGCCGTGCATGGGCAAAGGCTTGCATGTCATCGGAATCCACCATTGTTTACGTTCCAAACGGGAGGTTTTTGGTTCGGCCGATCGAGTTCCACGGCGGGTGTGGCAACAAGGATATTAGTTTCCATATCGACGGGGCTCTGGTTGCACCGTCCGATTATCGTATCCTTGGCGACCTCAGAAGCTGGCTGAGCTTTGATGGCGTTGATGGCGTTACTTTGAGTGGTGGAGTTCTTGATGCCAACGGCGCCGCCTTGTGGGACTGCAAATCCTCCGCCGCCCACTGCCCCGTCGGAGCCATGGTGCCTACCTCTAATTCTAGTTCTATTATTTAA

Coding sequence (CDS)

ATGGCCAAATTTTCCAACCATTCATCACTTCCCTCGCCATTTCAACCATTTATCGTCATACTCGTCATCGTCTTCTTCCTTTTCCTTACGATCACAACGTCATACTCTGCGACTTTATTGAACATTTTGGATTTCGGTGCCATACGCGGCGGGGGACGGGATTCGAGCCCGGCCTTTCGCCGTGCATGGGCAAAGGCTTGCATGTCATCGGAATCCACCATTGTTTACGTTCCAAACGGGAGGTTTTTGGTTCGGCCGATCGAGTTCCACGGCGGGTGTGGCAACAAGGATATTAGTTTCCATATCGACGGGGCTCTGGTTGCACCGTCCGATTATCGTATCCTTGGCGACCTCAGAAGCTGGCTGAGCTTTGATGGCGTTGATGGCGTTACTTTGAGTGGTGGAGTTCTTGATGCCAACGGCGCCGCCTTGTGGGACTGCAAATCCTCCGCCGCCCACTGCCCCGTCGGAGCCATGGTGCCTACCTCTAATTCTAGTTCTATTATTTAA

Protein sequence

MAKFSNHSSLPSPFQPFIVILVIVFFLFLTITTSYSATLLNILDFGAIRGGGRDSSPAFRRAWAKACMSSESTIVYVPNGRFLVRPIEFHGGCGNKDISFHIDGALVAPSDYRILGDLRSWLSFDGVDGVTLSGGVLDANGAALWDCKSSAAHCPVGAMVPTSNSSSII

Homology

[Close]

BLAST of Carg17810 vs. NCBI nr
Match: XP_022960566.1 (polygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 293.1 bits (749), Expect = 6.0e-76
Identity = 143/169 (84.62%), Postives = 147/169 (86.98%), Query Frame = 0


Query: 1   MAKFSNHSSLPSPFQPFIVXXXXXXXXXXXXXTSYSATLLNILDFGAIRGGGRDSSPAFR 60
           MAKFSNHSSLPSPFQPFIV              SYSATLLN+LDFGAI GGGRDSSPAFR
Sbjct: 1   MAKFSNHSSLPSPFQPFIVILVLVFLLFHMITKSYSATLLNVLDFGAIHGGGRDSSPAFR 60

Query: 61  RAWAKACMSSESTIVYVPNGRFLVRPIEFHGGCGNKDISFHIDGALVAPSDYRILGDLRS 120
           RAWAKACMSSESTIVYVPNGRFLV PIEFHGGCGNKDISFHIDGALVAPSDYRILGD+RS
Sbjct: 61  RAWAKACMSSESTIVYVPNGRFLVWPIEFHGGCGNKDISFHIDGALVAPSDYRILGDVRS 120

Query: 121 WLSFDGVDGVTLSGGVLDANGAALWDCKSSAAHCPVGAMVPTSNSSSII 170
           WLSFDGVDGVTLSGGVLDANGAALWDCKSSAAHCPVGAM     +S+ I
Sbjct: 121 WLSFDGVDGVTLSGGVLDANGAALWDCKSSAAHCPVGAMTLNFRNSNNI 169

[Close]

BLAST of Carg17810 vs. NCBI nr
Match: XP_023515405.1 (polygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 290.0 bits (741), Expect = 5.1e-75
Identity = 141/169 (83.43%), Postives = 146/169 (86.39%), Query Frame = 0


Query: 1   MAKFSNHSSLPSPFQPFIVXXXXXXXXXXXXXTSYSATLLNILDFGAIRGGGRDSSPAFR 60
           MAKFSNHSSLPSPFQPFIV             TSYSATLLN+LDFGAIRGGGRDSSPAFR
Sbjct: 1   MAKFSNHSSLPSPFQPFIVILVIVFFLFHTITTSYSATLLNVLDFGAIRGGGRDSSPAFR 60

Query: 61  RAWAKACMSSESTIVYVPNGRFLVRPIEFHGGCGNKDISFHIDGALVAPSDYRILGDLRS 120
           RAWAKACMSSESTIVYVP GRFLVRPIEFHGGCGN DISFHIDGALVAPSDYRILGD+ S
Sbjct: 61  RAWAKACMSSESTIVYVPKGRFLVRPIEFHGGCGNNDISFHIDGALVAPSDYRILGDVGS 120

Query: 121 WLSFDGVDGVTLSGGVLDANGAALWDCKSSAAHCPVGAMVPTSNSSSII 170
           WLSFDGVDGVTLSGGVLDANGAALWDCK+SA HCPVGAM     +S+ I
Sbjct: 121 WLSFDGVDGVTLSGGVLDANGAALWDCKASADHCPVGAMTLNFRNSNNI 169

[Close]

BLAST of Carg17810 vs. NCBI nr
Match: XP_022987940.1 (polygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 283.5 bits (724), Expect = 4.7e-73
Identity = 138/169 (81.66%), Postives = 145/169 (85.80%), Query Frame = 0


Query: 1   MAKFSNHSSLPSPFQPFIVXXXXXXXXXXXXXTSYSATLLNILDFGAIRGGGRDSSPAFR 60
           MAKFSNHSS PSPFQPFIV             TS+SATLLN+LDFGAIRGGGRDSSPAFR
Sbjct: 1   MAKFSNHSSRPSPFQPFIVILVVVFFLFHTITTSHSATLLNVLDFGAIRGGGRDSSPAFR 60

Query: 61  RAWAKACMSSESTIVYVPNGRFLVRPIEFHGGCGNKDISFHIDGALVAPSDYRILGDLRS 120
           RAWAKAC SSESTIVYVP GRFLVRPIEFHGGCGN DISFHIDGALVAPSDYRILGD+ S
Sbjct: 61  RAWAKACTSSESTIVYVPKGRFLVRPIEFHGGCGNNDISFHIDGALVAPSDYRILGDVGS 120

Query: 121 WLSFDGVDGVTLSGGVLDANGAALWDCKSSAAHCPVGAMVPTSNSSSII 170
           WLSFDGVDGV+LSGGVLDANGAALW+CKSSAAHCPVGAM     +S+ I
Sbjct: 121 WLSFDGVDGVSLSGGVLDANGAALWNCKSSAAHCPVGAMTLNFRNSNNI 169

[Close]

BLAST of Carg17810 vs. NCBI nr
Match: XP_008443656.2 (PREDICTED: polygalacturonase [Cucumis melo])

HSP 1 Score: 210.3 bits (534), Expect = 5.1e-51
Identity = 108/170 (63.53%), Postives = 121/170 (71.18%), Query Frame = 0


Query: 1   MAKFSNHSSLPSPFQPFIVXXXXXXXXXXXXXTSYSATLLNILDFGAIRGGGRDSSPAFR 60
           MAK SNHSS  SPFQP I+              S+SA LLNILDFGAIR  G DSS A  
Sbjct: 13  MAKISNHSSFHSPFQPTIILLALVILISHTIRRSHSANLLNILDFGAIR--GYDSSQAIH 72

Query: 61  RAWAKACMSSESTIVYVPNGRFLVRPIEFH-GGCGNKDISFHIDGALVAPSDYRILGDLR 120
           RAWA AC S ESTIVY+P GRFLV+PIEFH GGC N+DISFHIDGAL+ P DYRILG++ 
Sbjct: 73  RAWAVACKSEESTIVYIPKGRFLVQPIEFHGGGCHNEDISFHIDGALIGPPDYRILGNVE 132

Query: 121 SWLSFDGVDGVTLSGGVLDANGAALWDCKSSAAHCPVGAMVPTSNSSSII 170
           SWLSF  V+GV+L+GGVL ANG ALW CK S  HCPVGA   +   S+ I
Sbjct: 133 SWLSFVEVNGVSLTGGVLYANGEALWSCKFSTTHCPVGARTLSFRDSNNI 180

[Close]

BLAST of Carg17810 vs. NCBI nr
Match: XP_022150002.1 (polygalacturonase-like [Momordica charantia])

HSP 1 Score: 191.8 bits (486), Expect = 1.9e-45
Identity = 104/171 (60.82%), Postives = 120/171 (70.18%), Query Frame = 0


Query: 1   MAKFSNHSSLPSPFQPFIVXXXXXXXXXXXXXTSYSATLLNILDFGAIRGGGRDSSPAFR 60
           MAK S+  SLP    P ++              S+SATLLN+LDFG    G  D +  FR
Sbjct: 1   MAKISSTLSLP----PSLLHSIFLILLLGAISKSHSATLLNVLDFGGRHTG--DWTVPFR 60

Query: 61  RAWAKACMSSESTIVYVPNGRFLVRPIEFH-GGCGNKDISFHIDGALVAPSDYRILGDLR 120
           RAWA AC SSES IVY+P GR++VRP  FH GGC N DISFHIDGALVAP+DYRILG   
Sbjct: 61  RAWAAACSSSESAIVYIPKGRYVVRPTIFHGGGCENGDISFHIDGALVAPADYRILGGGE 120

Query: 121 SWLSFDGVDGVTLSGGVLDANGAALWDCKSS-AAHCPVGAMVPTSNSSSII 170
           SWLSF+GVDG+++SGGVLDANGAALW CKSS AAHCPVGAM  +  +S  I
Sbjct: 121 SWLSFEGVDGISVSGGVLDANGAALWACKSSAAAHCPVGAMTLSFRNSDNI 165

[Close]

BLAST of Carg17810 vs. TAIR10
Match: AT2G43870.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 136.3 bits (342), Expect = 1.7e-32
Identity = 66/136 (48.53%), Postives = 85/136 (62.50%), Query Frame = 0


Query: 33  TSYSATLLNILDFGAIRGGGRDSSPAFRRAWAKACMSSESTIVYVPNGRFLVRPIEFHGG 92
           +S SA   N+L FGA   G  D++ AF   W  AC SS    + VP GRFL+R + F G 
Sbjct: 14  SSCSAQSYNVLSFGAKPDGKTDATKAFMAVWQTACASSRPVTIVVPKGRFLLRSVTFDGS 73

Query: 93  -CGNKDISFHIDGALVAPSDYRILGDLRSWLSFDGVDGVTLSGGVLDANGAALWDCKSSA 152
            C  K ++F IDG LVAP+DYR++G+   W+ F  +DG+T+ GGVLDA GA+LWDCK S 
Sbjct: 74  KCKPKPVTFRIDGTLVAPADYRVIGNEDYWIFFQHLDGITVYGGVLDARGASLWDCKKSG 133

Query: 153 AHCPVGAMVPTSNSSS 168
            +CP GA      SSS
Sbjct: 134 KNCPSGATTIGFQSSS 149

[Close]

BLAST of Carg17810 vs. TAIR10
Match: AT3G59850.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 133.3 bits (334), Expect = 1.4e-31
Identity = 64/127 (50.39%), Postives = 81/127 (63.78%), Query Frame = 0


Query: 33  TSYSATLLNILDFGAIRGGGRDSSPAFRRAWAKACMSSESTIVYVPNGRFLVRPIEFHGG 92
           +S SA   NIL +GA   G  DS+ AF   WAKAC S +   + VP GRFL+R I F G 
Sbjct: 16  SSSSAQTYNILSYGAKPDGKTDSTKAFTVLWAKACASVKPVTILVPKGRFLLRSIIFDGS 75

Query: 93  -CGNKDISFHIDGALVAPSDYRILGDLRSWLSFDGVDGVTLSGGVLDANGAALWDCKSSA 152
            C  K ++F I G LVAPSDYR++G    W+ F  +DG+++ GGVLDA GA+LW CK S 
Sbjct: 76  KCKRKSVTFRIQGTLVAPSDYRVIGKENYWILFQHLDGISVYGGVLDAQGASLWSCKKSG 135

Query: 153 AHCPVGA 159
            +CP GA
Sbjct: 136 KNCPSGA 142

[Close]

BLAST of Carg17810 vs. TAIR10
Match: AT1G65570.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 119.8 bits (299), Expect = 1.6e-27
Identity = 56/123 (45.53%), Postives = 76/123 (61.79%), Query Frame = 0


Query: 38  TLLNILDFGAIRGGGRDSSPAFRRAWAKACMSSESTIVYVPNGRFLVR-PIEFHG-GCGN 97
           T LN+L FGA   G  +S+ AF  AW  AC   +S ++YVP GR+LV   + F G  C +
Sbjct: 29  TSLNVLSFGANPNGIVESAKAFSDAWDAACGVEDSVVIYVPKGRYLVSGEVRFEGESCKS 88

Query: 98  KDISFHIDGALVAPSDYRILGDLRSWLSFDGVDGVTLSGGVLDANGAALWDCKSSAAHCP 157
           ++I+  IDG L+ P DY +LG   +W SF GV  VT+ GG  DA G+ LW CK++  +CP
Sbjct: 89  REITLRIDGTLIGPQDYSLLGKKENWFSFSGVHNVTVLGGSFDAKGSTLWSCKANGYNCP 148

Query: 158 VGA 159
            GA
Sbjct: 149 EGA 151

[Close]

BLAST of Carg17810 vs. TAIR10
Match: AT2G43890.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 119.8 bits (299), Expect = 1.6e-27
Identity = 58/124 (46.77%), Postives = 78/124 (62.90%), Query Frame = 0


Query: 41  NILDFGAIRGGGRDSSPAFRRAWAKACMSSESTIVYVPNGRFLVRPIEFHGGCGNKDISF 100
           N++ FGA   G  DS+ AF  AW  AC S+ +  V VP G FL++P+EF G C ++ I+F
Sbjct: 30  NVVSFGAKPDGRTDSTKAFLGAWQAACRSAAAVTVTVPRGSFLLKPVEFRGPCRSR-ITF 89

Query: 101 HIDGALVAPSDYRILGDLRSWLSFDGVDGVTLSGGVLDANGAALWDCKSSAAHCPVGAMV 160
            I G +VAPSDYR LG+   W+ F  V+ +++ GG LDA GA+ W C+ S   CPVGA  
Sbjct: 90  QIYGTIVAPSDYRGLGNSGYWILFVKVNRISIIGGTLDARGASFWACRKSGKSCPVGARS 149

Query: 161 PTSN 165
            T N
Sbjct: 150 MTFN 152

[Close]

BLAST of Carg17810 vs. TAIR10
Match: AT2G43860.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 119.8 bits (299), Expect = 1.6e-27
Identity = 56/121 (46.28%), Postives = 76/121 (62.81%), Query Frame = 0


Query: 40  LNILDFGAIRGGGRDSSPAFRRAWAKACMSSESTIVYVPNGRFLVRPIEFHGG-CGNKDI 99
           LN+L +GA   G +DS+ AF  AW  AC S+  T + VP GRFLV  + FHG  C    I
Sbjct: 33  LNVLSYGAKPDGSKDSTKAFLAAWDVACASANPTTIIVPKGRFLVGNLVFHGNECKQAPI 92

Query: 100 SFHIDGALVAPSDYRILGDLRSWLSFDGVDGVTLSGGVLDANGAALWDCKSSAAH-CPVG 159
           S  I G++VAP D+RI+   + W+ F+ V  V++ GG+LDA G +LW CK++  H CP G
Sbjct: 93  SIRIAGSIVAPEDFRIIASSKHWIWFEDVTDVSIYGGILDAQGTSLWKCKNNGGHNCPTG 152

[Close]

BLAST of Carg17810 vs. Swiss-Prot
Match: sp|P48979|PGLR_PRUPE (Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.9e-28
Identity = 62/131 (47.33%), Postives = 83/131 (63.36%), Query Frame = 0


Query: 41  NILDFGAIRGGGRDSSPAFRRAWAKACMSSESTIVYVPNGRFLVRPIEFHGGCGNKDISF 100
           N+   GA   G  DS+ AF  AWAKAC S    ++YVP G F +R + F G C N  I+F
Sbjct: 29  NVASLGAKADGKTDSTKAFLSAWAKACASMNPGVIYVPAGTFFLRDVVFSGPCKNNAITF 88

Query: 101 HIDGALVAPSDYRILGDLRSWLSFDGVDGVTLSGGVLDANGAALWDCKS-SAAHCPVGA- 160
            I G LVAPSDYR++G+  +W+ F  V+GVT+SGG+LD  G ALW CK+     CP GA 
Sbjct: 89  RIAGTLVAPSDYRVIGNAANWIFFHHVNGVTISGGILDGQGTALWACKACHGESCPSGAT 148

Query: 161 MVPTSNSSSII 170
            +  S+S++I+
Sbjct: 149 TLGFSDSNNIV 159

[Close]

BLAST of Carg17810 vs. Swiss-Prot
Match: sp|O22818|PGLR6_ARATH (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.0e-26
Identity = 56/121 (46.28%), Postives = 76/121 (62.81%), Query Frame = 0


Query: 40  LNILDFGAIRGGGRDSSPAFRRAWAKACMSSESTIVYVPNGRFLVRPIEFHGG-CGNKDI 99
           LN+L +GA   G +DS+ AF  AW  AC S+  T + VP GRFLV  + FHG  C    I
Sbjct: 33  LNVLSYGAKPDGSKDSTKAFLAAWDVACASANPTTIIVPKGRFLVGNLVFHGNECKQAPI 92

Query: 100 SFHIDGALVAPSDYRILGDLRSWLSFDGVDGVTLSGGVLDANGAALWDCKSSAAH-CPVG 159
           S  I G++VAP D+RI+   + W+ F+ V  V++ GG+LDA G +LW CK++  H CP G
Sbjct: 93  SIRIAGSIVAPEDFRIIASSKHWIWFEDVTDVSIYGGILDAQGTSLWKCKNNGGHNCPTG 152

[Close]

BLAST of Carg17810 vs. Swiss-Prot
Match: sp|P48978|PGLR_MALDO (Polygalacturonase OS=Malus domestica OX=3750 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 3.6e-16
Identity = 50/134 (37.31%), Postives = 70/134 (52.24%), Query Frame = 0


Query: 33  TSYSATLLNILDFGAIRGGGRDSSPAFRRAWAKACMSSESTIVYVPNGRFLVRPIEFHGG 92
           +S  A  +++ DFGA +G G D + AF +AW  AC SS + ++ VP   +LVRPIEF G 
Sbjct: 92  SSAPAKTISVDDFGA-KGNGADDTQAFVKAWKAACSSSGAMVLVVPQKNYLVRPIEFSGP 151

Query: 93  CGNKDISFHIDGALVAPSDYRILGDLRSWLSFDGVDGVTLSG-GVLDANGAALW--DCKS 152
           C    ++  I G + A  D  I  D+  WL FD V  + + G G ++ NG   W   CK 
Sbjct: 152 C-KSQLTLQIYGTIEASEDRSIYKDIDHWLIFDNVQNLLVVGPGTINGNGNIWWKNSCKI 211

Query: 153 SAAHCPVGAMVPTS 164
                P G   PT+
Sbjct: 212 K-PQPPCGTYAPTA 222

[Close]

BLAST of Carg17810 vs. Swiss-Prot
Match: sp|P35336|PGLR_ACTDE (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.4e-15
Identity = 49/119 (41.18%), Postives = 67/119 (56.30%), Query Frame = 0


Query: 40  LNILDFGAIRGGGRDSSPAFRRAWAKACMSSESTIVYVPNGRFLVRPIEFHGGCGNKDIS 99
           +N+ DFGA +G GRD + AF +AW  AC S+ S ++ VP   +LVRPI F G C    ++
Sbjct: 90  VNVDDFGA-KGDGRDDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGPC-KSGLT 149

Query: 100 FHIDGALVAP---SDYRILGDLRSWLSFDGVDGVTL-SGGVLDANGAALW--DCKSSAA 153
             I G + A    SDYR   D R WL FD V  + +  GG ++ NG   W   CK++ A
Sbjct: 150 MQIYGTIEASDDRSDYR--KDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKA 204

[Close]

BLAST of Carg17810 vs. Swiss-Prot
Match: sp|Q8RY29|ADPG2_ARATH (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 75.5 bits (184), Expect = 6.4e-13
Identity = 46/124 (37.10%), Postives = 68/124 (54.84%), Query Frame = 0


Query: 38  TLLNILDFGAIRGGGRDSSPAFRRAWAKACMSSESTIVYVPNGR-FLVRPIEFHGGCGNK 97
           T +++ DFGA   G  D + AF  AW KAC S+ +  + VP G  +L++ I+  G C N 
Sbjct: 66  TTVSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSIQLTGPC-NS 125

Query: 98  DISFHIDGALVAP---SDYRILGDLRSWLSFDGVDGVTLSG---GVLDANGAALW--DCK 153
            ++  I G L A    SDY+   D+  W+ FDGV+ +++ G   GV+D NG   W   CK
Sbjct: 126 ILTVQIFGTLSASQKRSDYK---DISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQNSCK 185

[Close]

BLAST of Carg17810 vs. TrEMBL
Match: tr|A0A1S3B8I8|A0A1S3B8I8_CUCME (polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103487206 PE=3 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.4e-51
Identity = 108/170 (63.53%), Postives = 121/170 (71.18%), Query Frame = 0


Query: 1   MAKFSNHSSLPSPFQPFIVXXXXXXXXXXXXXTSYSATLLNILDFGAIRGGGRDSSPAFR 60
           MAK SNHSS  SPFQP I+              S+SA LLNILDFGAIR  G DSS A  
Sbjct: 13  MAKISNHSSFHSPFQPTIILLALVILISHTIRRSHSANLLNILDFGAIR--GYDSSQAIH 72

Query: 61  RAWAKACMSSESTIVYVPNGRFLVRPIEFH-GGCGNKDISFHIDGALVAPSDYRILGDLR 120
           RAWA AC S ESTIVY+P GRFLV+PIEFH GGC N+DISFHIDGAL+ P DYRILG++ 
Sbjct: 73  RAWAVACKSEESTIVYIPKGRFLVQPIEFHGGGCHNEDISFHIDGALIGPPDYRILGNVE 132

Query: 121 SWLSFDGVDGVTLSGGVLDANGAALWDCKSSAAHCPVGAMVPTSNSSSII 170
           SWLSF  V+GV+L+GGVL ANG ALW CK S  HCPVGA   +   S+ I
Sbjct: 133 SWLSFVEVNGVSLTGGVLYANGEALWSCKFSTTHCPVGARTLSFRDSNNI 180

[Close]

BLAST of Carg17810 vs. TrEMBL
Match: tr|A0A2N9IUU6|A0A2N9IUU6_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS57049 PE=3 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.7e-34
Identity = 70/137 (51.09%), Postives = 99/137 (72.26%), Query Frame = 0


Query: 33  TSYSATLLNILDFGAIRGGGRDSSPAFRRAWAKACMSSESTIVYVPNGRFLVRPIEFHGG 92
           TS++  + N+L+FGA   G +DS+PAFR AW+ AC SS+S+ + VP GR+L+  + F G 
Sbjct: 20  TSHATIVYNVLNFGAKPDGIKDSTPAFRNAWSAACASSDSSTINVPKGRYLLGSLAFKGN 79

Query: 93  CGNKDISFHIDGALVAPSDYRILGDLRSWLSFDGVDGVTLSGGVLDANGAALWDCKSSAA 152
           C + DI+F IDG LVAP+DYR+LG   +WLSF+G+ GVT+ GG LDA G+ALW CK++  
Sbjct: 80  CKSSDITFRIDGTLVAPADYRVLGKASNWLSFEGISGVTIIGGALDAKGSALWACKAART 139

Query: 153 HCPVGA-MVPTSNSSSI 169
            CP GA  +  +NS++I
Sbjct: 140 DCPKGATTLSFTNSNNI 156

[Close]

BLAST of Carg17810 vs. TrEMBL
Match: tr|A0A067DFA5|A0A067DFA5_CITSI (Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g042768mg PE=3 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 3.2e-33
Identity = 69/134 (51.49%), Postives = 95/134 (70.90%), Query Frame = 0


Query: 36  SATLLNILDFGAIRGGGRDSSPAFRRAWAKACMSSESTIVYVPNGRFLVRPIEFHGGCGN 95
           + T  N+L FGA   G  DS+ AF +AWA AC S+ES  +YVP GR+L+  + F+G C +
Sbjct: 29  TTTTYNVLSFGAKGNGVTDSTQAFAKAWAAACASTESATLYVPKGRYLLGSVAFNGDCKS 88

Query: 96  KDISFHIDGALVAPSDYRILGDLRSWLSFDGVDGVTLSGGVLDANGAALWDCKSSAAHCP 155
            DI+F IDG LVAP+DYR+LG   +WLSF+GV GV++ GG LDA G++LW CK+S  +CP
Sbjct: 89  SDITFRIDGTLVAPADYRVLGQADNWLSFEGVSGVSIIGGALDAKGSSLWACKASGTNCP 148

Query: 156 VGA-MVPTSNSSSI 169
            GA  +  +NS++I
Sbjct: 149 DGATTLSFTNSNNI 162

[Close]

BLAST of Carg17810 vs. TrEMBL
Match: tr|A0A2C9WKE5|A0A2C9WKE5_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_01G088400 PE=3 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.6e-32
Identity = 69/137 (50.36%), Postives = 94/137 (68.61%), Query Frame = 0


Query: 33  TSYSATLLNILDFGAIRGGGRDSSPAFRRAWAKACMSSESTIVYVPNGRFLVRPIEFHGG 92
           +S++ +  N+L +GA   G  DS+ AF  AWA AC S  ST++YVPNGR+L+ PI F G 
Sbjct: 19  SSFARSTFNVLSYGAKPNGATDSTKAFLDAWAAACSSVGSTMIYVPNGRYLLGPIIFRGA 78

Query: 93  CGNKDISFHIDGALVAPSDYRILGDLRSWLSFDGVDGVTLSGGVLDANGAALWDCKSSAA 152
           C + DI+  IDG LVAP DY ILG   +WLSF+GV GV++ GG LDA G+ LW CK+  +
Sbjct: 79  CKSSDITIRIDGTLVAPGDYHILGQAANWLSFEGVAGVSIVGGALDAKGSPLWACKAKGS 138

Query: 153 HCPVGA-MVPTSNSSSI 169
           +CP GA  +  +NS++I
Sbjct: 139 NCPNGATTLSFTNSNNI 155

[Close]

BLAST of Carg17810 vs. TrEMBL
Match: tr|B9SEE6|B9SEE6_RICCO (Polygalacturonase, putative OS=Ricinus communis OX=3988 GN=RCOM_0703170 PE=3 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 3.5e-32
Identity = 69/137 (50.36%), Postives = 93/137 (67.88%), Query Frame = 0


Query: 33  TSYSATLLNILDFGAIRGGGRDSSPAFRRAWAKACMSSESTIVYVPNGRFLVRPIEFHGG 92
           +S++    N+L +GA   G  DS+ AF  AWA AC S++ST++YVP GR+L+  + F GG
Sbjct: 16  SSFATDNYNVLSYGAKPNGITDSTKAFLDAWAAACGSTDSTMIYVPKGRYLLGSMSFKGG 75

Query: 93  CGNKDISFHIDGALVAPSDYRILGDLRSWLSFDGVDGVTLSGGVLDANGAALWDCKSSAA 152
           C +  I+  IDG LVAP DY ILG   SWLSF+GVDGV++ GG LDA G+ LWDCK+   
Sbjct: 76  CKSHSITIRIDGTLVAPEDYLILGKATSWLSFEGVDGVSIIGGALDAKGSTLWDCKAKGT 135

Query: 153 HCPVGA-MVPTSNSSSI 169
            CP GA  +  +NS++I
Sbjct: 136 DCPTGATTLRFTNSNNI 152

The following BLAST results are available for this feature:

BLAST of Carg17810 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022960566.1	6.0e-76	84.62	polygalacturonase-like [Cucurbita moschata]	[more]
XP_023515405.1	5.1e-75	83.43	polygalacturonase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022987940.1	4.7e-73	81.66	polygalacturonase-like [Cucurbita maxima]	[more]
XP_008443656.2	5.1e-51	63.53	PREDICTED: polygalacturonase [Cucumis melo]	[more]
XP_022150002.1	1.9e-45	60.82	polygalacturonase-like [Momordica charantia]	[more]

BLAST of Carg17810 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G43870.1	1.7e-32	48.53	Pectin lyase-like superfamily protein	[more]
AT3G59850.1	1.4e-31	50.39	Pectin lyase-like superfamily protein	[more]
AT1G65570.1	1.6e-27	45.53	Pectin lyase-like superfamily protein	[more]
AT2G43890.1	1.6e-27	46.77	Pectin lyase-like superfamily protein	[more]
AT2G43860.1	1.6e-27	46.28	Pectin lyase-like superfamily protein	[more]

BLAST of Carg17810 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P48979\|PGLR_PRUPE	1.9e-28	47.33	Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1	[more]
sp\|O22818\|PGLR6_ARATH	3.0e-26	46.28	Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386...	[more]
sp\|P48978\|PGLR_MALDO	3.6e-16	37.31	Polygalacturonase OS=Malus domestica OX=3750 PE=2 SV=1	[more]
sp\|P35336\|PGLR_ACTDE	1.4e-15	41.18	Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1	[more]
sp\|Q8RY29\|ADPG2_ARATH	6.4e-13	37.10	Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2	[more]

BLAST of Carg17810 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3B8I8\|A0A1S3B8I8_CUCME	3.4e-51	63.53	polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103487206 PE=3 SV=1	[more]
tr\|A0A2N9IUU6\|A0A2N9IUU6_FAGSY	1.7e-34	51.09	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS57049 PE=3 SV=1	[more]
tr\|A0A067DFA5\|A0A067DFA5_CITSI	3.2e-33	51.49	Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g042768mg PE=3 SV=1	[more]
tr\|A0A2C9WKE5\|A0A2C9WKE5_MANES	1.6e-32	50.36	Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_01G088400 PE=3 SV=...	[more]
tr\|B9SEE6\|B9SEE6_RICCO	3.5e-32	50.36	Polygalacturonase, putative OS=Ricinus communis OX=3988 GN=RCOM_0703170 PE=3 SV=...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0004650	polygalacturonase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR011050	Pectin_lyase_fold/virulence
IPR000743	Glyco_hydro_28
IPR012334	Pectin_lyas_fold

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004650	polygalacturonase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg17810-RA	Carg17810-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 27..168 e-value: 9.8E-23 score: 82.7
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 67..151 e-value: 5.4E-6 score: 25.6
None	No IPR available	PANTHER	PTHR31375:SF49	F5I14.10	coord: 21..154
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 21..154
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	SSF51126	Pectin lyase-like	coord: 21..151

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg17810	MELO3C010017	Melon (DHL92) v3.5.1	carmeB0946
Carg17810	Cucsa.356350	Cucumber (Gy14) v1	carcgyB1085
Carg17810	CmaCh10G008450	Cucurbita maxima (Rimu)	carcmaB1335
Carg17810	CmoCh10G008790	Cucurbita moschata (Rifu)	carcmoB1297
Carg17810	Lsi06G006700	Bottle gourd (USVL1VR-Ls)	carlsiB886
Carg17810	Cp4.1LG18g02390	Cucurbita pepo (Zucchini)	carcpeB1341

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg17810	Melon (DHL92) v3.5.1	carmeB0947
Carg17810	Melon (DHL92) v3.6.1	carmedB0952
Carg17810	Melon (DHL92) v3.6.1	carmedB0953
Carg17810	Watermelon (Charleston Gray)	carwcgB0945
Carg17810	Watermelon (97103) v1	carwmB0970
Carg17810	Watermelon (97103) v2	carwmbB1021
Carg17810	Wax gourd	carwgoB1135
Carg17810	Silver-seed gourd	carcarB502
Carg17810	Cucumber (Gy14) v2	carcgybB0955
Carg17810	Cucurbita maxima (Rimu)	carcmaB1336
Carg17810	Cucurbita moschata (Rifu)	carcmoB1298
Carg17810	Cucurbita pepo (Zucchini)	carcpeB1340
Carg17810	Wild cucumber (PI 183967)	carcpiB1005
Carg17810	Cucumber (Chinese Long) v2	carcuB0982
Carg17810	Cucumber (Chinese Long) v3	carcucB1010