Carg16093 (gene) Silver-seed gourd

NameCarg16093
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionThymidine kinase
LocationCucurbita_argyrosperma_scaffold_068 : 55143 .. 56273 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GATCTCAGTCTCCTTCAAAATTGCGCCTCCGATTTCGATTCAAATTCTGTCCTATTTACTTTTTGTCTCCAATCTGCTCCATGGAGTCCTTGAGGAATTCATTTTCTTTGACCTCCACCGATGGTGGCGCCGTCAGTGCAGGATCTCCACACCGCGTCGCAGGCGAAGTCCATGTGATCATCGGTCCTATGTTTGCCGGAAAAACCACCGCTCTACTCCGTCGGATCAAAGCTGAGAGCAGCAGTGGCAGGTGAAGATAAAGTCTATTCTTACGGCTTGCAAACAGATCGAAAACCTGCATTAAAATCTCAACTTGGAATTTCTTTGACTCTCGTATCTGCTCTGCAGGAATGTGGTGATGATCAAGTCAAGCAAGGACACAAGGTACGCTATTGATTCGGTAGTAACACACGATGGGGCCAAATTTCCATGCTGGGCACTGCCAGATCTTTCGTCTTTCCGACAGAAATTTGGAGAAGATGCTTATAACAAGGTATTCCTCTTTGAAGAACATTCTTTGGCATTACTATTTGTTTTAGTTATCTAAACCCCCAATAATACCGGCAGCTGGATGTGATTGGTGTTGATGAAGCTCAATTTTTCGATGATCTCTATGATTTTTGCTGCGATGTTGCTGATAAAGATGACAAAATCATTATCGTTGCTGGCTTAGATGGAGATTACCTCAGGTCCCACTCAGATCTCTCTCTGTCTTTCTCTGTATATATGACAATTCTGCATTCACATAATTTCTTGCATCTGAGCTGTTGGGTAACTTGAAAATTGTGCCACCCAGATGCGAAATCATGGATCTTTCCAATACTAAAGTGGTTATTCTTCCATGGTTTTGGCAGGAGGAGATTTGGTTCAGTGCTTGATGTAGTTCCTCTGGCTGACACAGTAACTAAGTTGACAGCTAGATGTGAACTTTGTGGTAAAAGAGCCTTCTTTACCTTGAGAAAGACAGAGGAGACAAGGACCGAGCTTATCGCTGGGGCTGATGTCTATATGCCGGTGTGCCGCCACCACTATGCCAATGGACAAGAAGTCATACAATCTTCCAAGAATATTCTGGGATCTCGTGCTCTTAGAACAGGCTCTAATCTAGAGGCACTTCCTTCACTTATCTAA

mRNA sequence

GATCTCAGTCTCCTTCAAAATTGCGCCTCCGATTTCGATTCAAATTCTGTCCTATTTACTTTTTGTCTCCAATCTGCTCCATGGAGTCCTTGAGGAATTCATTTTCTTTGACCTCCACCGATGGTGGCGCCGTCAGTGCAGGATCTCCACACCGCGTCGCAGGCGAAGTCCATGTGATCATCGGTCCTATGTTTGCCGGAAAAACCACCGCTCTACTCCGTCGGATCAAAGCTGAGAGCAGCAGTGGCAGGAATGTGGTGATGATCAAGTCAAGCAAGGACACAAGGTACGCTATTGATTCGGTAGTAACACACGATGGGGCCAAATTTCCATGCTGGGCACTGCCAGATCTTTCGTCTTTCCGACAGAAATTTGGAGAAGATGCTTATAACAAGCTGGATGTGATTGGTGTTGATGAAGCTCAATTTTTCGATGATCTCTATGATTTTTGCTGCGATGTTGCTGATAAAGATGACAAAATCATTATCGTTGCTGGCTTAGATGGAGATTACCTCAGGAGGAGATTTGGTTCAGTGCTTGATGTAGTTCCTCTGGCTGACACAGTAACTAAGTTGACAGCTAGATGTGAACTTTGTGGTAAAAGAGCCTTCTTTACCTTGAGAAAGACAGAGGAGACAAGGACCGAGCTTATCGCTGGGGCTGATGTCTATATGCCGGTGTGCCGCCACCACTATGCCAATGGACAAGAAGTCATACAATCTTCCAAGAATATTCTGGGATCTCGTGCTCTTAGAACAGGCTCTAATCTAGAGGCACTTCCTTCACTTATCTAA

Coding sequence (CDS)

ATGGAGTCCTTGAGGAATTCATTTTCTTTGACCTCCACCGATGGTGGCGCCGTCAGTGCAGGATCTCCACACCGCGTCGCAGGCGAAGTCCATGTGATCATCGGTCCTATGTTTGCCGGAAAAACCACCGCTCTACTCCGTCGGATCAAAGCTGAGAGCAGCAGTGGCAGGAATGTGGTGATGATCAAGTCAAGCAAGGACACAAGGTACGCTATTGATTCGGTAGTAACACACGATGGGGCCAAATTTCCATGCTGGGCACTGCCAGATCTTTCGTCTTTCCGACAGAAATTTGGAGAAGATGCTTATAACAAGCTGGATGTGATTGGTGTTGATGAAGCTCAATTTTTCGATGATCTCTATGATTTTTGCTGCGATGTTGCTGATAAAGATGACAAAATCATTATCGTTGCTGGCTTAGATGGAGATTACCTCAGGAGGAGATTTGGTTCAGTGCTTGATGTAGTTCCTCTGGCTGACACAGTAACTAAGTTGACAGCTAGATGTGAACTTTGTGGTAAAAGAGCCTTCTTTACCTTGAGAAAGACAGAGGAGACAAGGACCGAGCTTATCGCTGGGGCTGATGTCTATATGCCGGTGTGCCGCCACCACTATGCCAATGGACAAGAAGTCATACAATCTTCCAAGAATATTCTGGGATCTCGTGCTCTTAGAACAGGCTCTAATCTAGAGGCACTTCCTTCACTTATCTAA

Protein sequence

MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVVMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDLYDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI
BLAST of Carg16093 vs. NCBI nr
Match: XP_023513380.1 (thymidine kinase a [Cucurbita pepo subsp. pepo])

HSP 1 Score: 470.7 bits (1210), Expect = 2.9e-129
Identity = 236/237 (99.58%), Postives = 236/237 (99.58%), Query Frame = 0

Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV
Sbjct: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL
Sbjct: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCCDVADKD KIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL
Sbjct: 121 YDFCCDVADKDGKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 238
           RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI
Sbjct: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 237

BLAST of Carg16093 vs. NCBI nr
Match: XP_022943895.1 (thymidine kinase a [Cucurbita moschata])

HSP 1 Score: 468.8 bits (1205), Expect = 1.1e-128
Identity = 235/237 (99.16%), Postives = 235/237 (99.16%), Query Frame = 0

Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           MESLRNSFSL STDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV
Sbjct: 1   MESLRNSFSLISTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL
Sbjct: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCCDVADKD KIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL
Sbjct: 121 YDFCCDVADKDSKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 238
           RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI
Sbjct: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 237

BLAST of Carg16093 vs. NCBI nr
Match: XP_022985744.1 (thymidine kinase-like [Cucurbita maxima])

HSP 1 Score: 459.9 bits (1182), Expect = 5.2e-126
Identity = 232/237 (97.89%), Postives = 233/237 (98.31%), Query Frame = 0

Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           MESLRNSF   STDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV
Sbjct: 1   MESLRNSF---STDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKDTRYA+DSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL
Sbjct: 61  MIKSSKDTRYAVDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCCDVADKD KIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL
Sbjct: 121 YDFCCDVADKDGKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 238
           RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI
Sbjct: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 234

BLAST of Carg16093 vs. NCBI nr
Match: XP_022140502.1 (thymidine kinase [Momordica charantia])

HSP 1 Score: 427.2 bits (1097), Expect = 3.7e-116
Identity = 212/236 (89.83%), Postives = 224/236 (94.92%), Query Frame = 0

Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           M+S R+S SLT+TDGG V AGS  RVAGEVHVIIGPMFAGKTTALLRRIK+ES+SGRNVV
Sbjct: 1   MDSFRSSLSLTTTDGGTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSGRNVV 60

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAY+KLDVIGVDEAQFFDDL
Sbjct: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDDL 120

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCC+VADKD KI+IVAGLDGDYLRR FGSVLDVVPLADTVTKLTARCELCGKRAFFTL
Sbjct: 121 YDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSL 237
           RKTEETRTELIAGADVYMPVCR HYANGQ+VIQ+SKN+LGSR + TGSNLEAL SL
Sbjct: 181 RKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVLGSRTVTTGSNLEALASL 236

BLAST of Carg16093 vs. NCBI nr
Match: XP_008464186.1 (PREDICTED: thymidine kinase [Cucumis melo])

HSP 1 Score: 409.8 bits (1052), Expect = 6.1e-111
Identity = 203/237 (85.65%), Postives = 219/237 (92.41%), Query Frame = 0

Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           MES R+S SLTS++  A+   S HR+ GEVHVIIGPMFAGKTTALLRRIK+ S+SGRNVV
Sbjct: 1   MESCRSSLSLTSSNADAIRPESSHRLTGEVHVIIGPMFAGKTTALLRRIKSASNSGRNVV 60

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKDTRYA+DSVVTHDGAKFPCWALP LSSFRQKFGEDAYN+LDVIGVDEAQFFDDL
Sbjct: 61  MIKSSKDTRYAVDSVVTHDGAKFPCWALPHLSSFRQKFGEDAYNELDVIGVDEAQFFDDL 120

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCC+VAD+D KII+VAGLDGDYLRR FGSVLDVVPLADTVTKLTARCELCGKRAFFTL
Sbjct: 121 YDFCCNVADRDGKIIVVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 238
           RKTEET+TELIAGADVYMPVCRHHYA GQEVIQSSKN++GSR +   SNLEALPSLI
Sbjct: 181 RKTEETKTELIAGADVYMPVCRHHYATGQEVIQSSKNVMGSRTI--ASNLEALPSLI 235

BLAST of Carg16093 vs. TAIR10
Match: AT3G07800.1 (Thymidine kinase)

HSP 1 Score: 308.9 bits (790), Expect = 2.7e-84
Identity = 151/222 (68.02%), Postives = 179/222 (80.63%), Query Frame = 0

Query: 1   MESLRNSFSLTSTDG---GAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGR 60
           M +L+ SF + + D    G   +    R +G VHVI+GPMF+GK+T+LLRRIK+E S GR
Sbjct: 1   MATLKASFLIKTLDSDVTGDFLSDLERRGSGAVHVIMGPMFSGKSTSLLRRIKSEISDGR 60

Query: 61  NVVMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFF 120
           +V M+KSSKDTRYA DSVVTHDG  FPCWALPDL SF +KFG DAYNKLDVIG+DEAQFF
Sbjct: 61  SVAMLKSSKDTRYAKDSVVTHDGIGFPCWALPDLMSFPEKFGLDAYNKLDVIGIDEAQFF 120

Query: 121 DDLYDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAF 180
            DLY+FCC VAD D KI+IVAGLDGDYLRR FG+VLD++P+AD+VTKLTARCE+CG +AF
Sbjct: 121 GDLYEFCCKVADDDGKIVIVAGLDGDYLRRSFGAVLDIIPIADSVTKLTARCEVCGHKAF 180

Query: 181 FTLRKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNIL 220
           FTLRK  +TRTELI GADVYMPVCR HY     VI++SK +L
Sbjct: 181 FTLRKNCDTRTELIGGADVYMPVCRKHYITNHIVIKASKKVL 222

BLAST of Carg16093 vs. TAIR10
Match: AT5G23070.1 (Thymidine kinase)

HSP 1 Score: 293.9 bits (751), Expect = 8.9e-80
Identity = 135/212 (63.68%), Postives = 176/212 (83.02%), Query Frame = 0

Query: 11  TSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVVMIKSSKDTRY 70
           +S+    +S+ SP    GE+HV++GPMF+GKTT LLRRI AE  +G+ + +IKS+KDTRY
Sbjct: 64  SSSSSQPLSSSSP----GEIHVVVGPMFSGKTTTLLRRILAERETGKRIAIIKSNKDTRY 123

Query: 71  AIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAY-NKLDVIGVDEAQFFDDLYDFCCDVAD 130
             +S+VTHDG K+PCW+LPDLSSF+++FG D Y N+LDVIG+DEAQFF DLY+FC + AD
Sbjct: 124 CTESIVTHDGEKYPCWSLPDLSSFKERFGFDDYENRLDVIGIDEAQFFGDLYEFCREAAD 183

Query: 131 KDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTE 190
           K+ K +IVAGLDGD++RRRFGSVLD++P+ADTVTKLT+RCE+CGKRA FT+RKTEE  TE
Sbjct: 184 KEGKTVIVAGLDGDFMRRRFGSVLDLIPIADTVTKLTSRCEVCGKRALFTMRKTEEKETE 243

Query: 191 LIAGADVYMPVCRHHYANGQEVIQSSKNILGS 222
           LI GA+VYMPVCR HY  GQ V+++++ +L S
Sbjct: 244 LIGGAEVYMPVCRSHYVCGQNVLETARAVLDS 271

BLAST of Carg16093 vs. Swiss-Prot
Match: sp|Q9S750|KITHA_ARATH (Thymidine kinase a OS=Arabidopsis thaliana OX=3702 GN=TK1A PE=1 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 4.8e-83
Identity = 151/222 (68.02%), Postives = 179/222 (80.63%), Query Frame = 0

Query: 1   MESLRNSFSLTSTDG---GAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGR 60
           M +L+ SF + + D    G   +    R +G VHVI+GPMF+GK+T+LLRRIK+E S GR
Sbjct: 1   MATLKASFLIKTLDSDVTGDFLSDLERRGSGAVHVIMGPMFSGKSTSLLRRIKSEISDGR 60

Query: 61  NVVMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFF 120
           +V M+KSSKDTRYA DSVVTHDG  FPCWALPDL SF +KFG DAYNKLDVIG+DEAQFF
Sbjct: 61  SVAMLKSSKDTRYAKDSVVTHDGIGFPCWALPDLMSFPEKFGLDAYNKLDVIGIDEAQFF 120

Query: 121 DDLYDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAF 180
            DLY+FCC VAD D KI+IVAGLDGDYLRR FG+VLD++P+AD+VTKLTARCE+CG +AF
Sbjct: 121 GDLYEFCCKVADDDGKIVIVAGLDGDYLRRSFGAVLDIIPIADSVTKLTARCEVCGHKAF 180

Query: 181 FTLRKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNIL 220
           FTLRK  +TRTELI GADVYMPVCR HY     VI++SK +L
Sbjct: 181 FTLRKNCDTRTELIGGADVYMPVCRKHYITNHIVIKASKKVL 222

BLAST of Carg16093 vs. Swiss-Prot
Match: sp|O81263|KITH_ORYSJ (Thymidine kinase OS=Oryza sativa subsp. japonica OX=39947 GN=TK PE=2 SV=2)

HSP 1 Score: 308.5 bits (789), Expect = 6.3e-83
Identity = 140/193 (72.54%), Postives = 170/193 (88.08%), Query Frame = 0

Query: 28  GEVHVIIGPMFAGKTTALLRRIKAESSSG-RNVVMIKSSKDTRYAIDSVVTHDGAKFPCW 87
           GE+HVI+GPMFAGKTTALLRR++ E+ +G RNV +IKS KD RY +DSVVTHDG K PCW
Sbjct: 67  GEIHVIVGPMFAGKTTALLRRVQVEAGTGSRNVALIKSDKDNRYGLDSVVTHDGTKMPCW 126

Query: 88  ALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDLYDFCCDVADKDDKIIIVAGLDGDYLR 147
           ALP+LSSF+ K G +AY+K+DVIG+DEAQFFDDL+DFCC  AD+D KI++VAGLDGDY R
Sbjct: 127 ALPELSSFQDKLGTEAYDKVDVIGIDEAQFFDDLHDFCCKAADRDGKIVVVAGLDGDYKR 186

Query: 148 RRFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTELIAGADVYMPVCRHHYA 207
            +FGSVLD++PLAD+VTKLTARCELCG+RAFFTLRKT ET+TELI GADVYMPVCR HY 
Sbjct: 187 NKFGSVLDIIPLADSVTKLTARCELCGRRAFFTLRKTRETKTELIGGADVYMPVCRQHYL 246

Query: 208 NGQEVIQSSKNIL 220
           +GQ VI++++ +L
Sbjct: 247 DGQIVIEATRIVL 259

BLAST of Carg16093 vs. Swiss-Prot
Match: sp|F4KBF5|KITHB_ARATH (Thymidine kinase b OS=Arabidopsis thaliana OX=3702 GN=TK1B PE=1 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 1.6e-78
Identity = 135/212 (63.68%), Postives = 176/212 (83.02%), Query Frame = 0

Query: 11  TSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVVMIKSSKDTRY 70
           +S+    +S+ SP    GE+HV++GPMF+GKTT LLRRI AE  +G+ + +IKS+KDTRY
Sbjct: 64  SSSSSQPLSSSSP----GEIHVVVGPMFSGKTTTLLRRILAERETGKRIAIIKSNKDTRY 123

Query: 71  AIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAY-NKLDVIGVDEAQFFDDLYDFCCDVAD 130
             +S+VTHDG K+PCW+LPDLSSF+++FG D Y N+LDVIG+DEAQFF DLY+FC + AD
Sbjct: 124 CTESIVTHDGEKYPCWSLPDLSSFKERFGFDDYENRLDVIGIDEAQFFGDLYEFCREAAD 183

Query: 131 KDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTE 190
           K+ K +IVAGLDGD++RRRFGSVLD++P+ADTVTKLT+RCE+CGKRA FT+RKTEE  TE
Sbjct: 184 KEGKTVIVAGLDGDFMRRRFGSVLDLIPIADTVTKLTSRCEVCGKRALFTMRKTEEKETE 243

Query: 191 LIAGADVYMPVCRHHYANGQEVIQSSKNILGS 222
           LI GA+VYMPVCR HY  GQ V+++++ +L S
Sbjct: 244 LIGGAEVYMPVCRSHYVCGQNVLETARAVLDS 271

BLAST of Carg16093 vs. Swiss-Prot
Match: sp|P23335|KITH_SWPVK (Thymidine kinase OS=Swinepox virus (strain Kasza) OX=10277 GN=TK PE=3 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 4.1e-34
Identity = 74/178 (41.57%), Postives = 112/178 (62.92%), Query Frame = 0

Query: 28  GEVHVIIGPMFAGKTTALLRRIKAESSSGRNVVMIKSSKDTRYAIDSVVTHDGAKFPCWA 87
           G +H+I+GPMF+GK+T L+R +     +  N  +IK SKD RY  D+V THD       +
Sbjct: 4   GFIHLILGPMFSGKSTELIRLVNRYQIATYNCRVIKYSKDNRYGNDAVYTHDKCYISAVS 63

Query: 88  LPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDLYDFCCDVADKDDKIIIVAGLDGDYLRR 147
              L   +     D  + +D++G+DE QFF+D+ +FC  +A+K  KI+IVA LDG Y R+
Sbjct: 64  TDSLFDIK-----DTLDDVDIVGIDEGQFFNDIVEFCEYIANK-GKIVIVAALDGTYERK 123

Query: 148 RFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTELIAGADVYMPVCRHHY 206
            FG++L+++PL++ VTKL A C +C + A F+ R ++E   ELI G + Y+ VCR  Y
Sbjct: 124 PFGNILNLIPLSEKVTKLNAICMICHRDASFSKRLSDEKEIELIGGKEKYLSVCRSCY 175

BLAST of Carg16093 vs. Swiss-Prot
Match: sp|Q27564|KITH_DICDI (Thymidine kinase 1 OS=Dictyostelium discoideum OX=44689 GN=thyB PE=1 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 9.2e-34
Identity = 87/199 (43.72%), Postives = 119/199 (59.80%), Query Frame = 0

Query: 25  RVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVVMIKSSKDTRY--AIDS--VVTHDG 84
           ++AG++ VI GPMF+GKTT L+RRIK  + + +  ++IK SKDTRY   ID   +VTHD 
Sbjct: 5   QIAGKIQVIFGPMFSGKTTELIRRIKRFNFANKKCLLIKYSKDTRYNDNIDKSFLVTHDK 64

Query: 85  AK---FPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDLYDFCCDVADKDDKIIIV 144
                FPC  L D+    Q +        DVIG+DE QFF D+  F  D+A++  K +I+
Sbjct: 65  QNYQAFPCSILEDVKEQAQNY--------DVIGIDEGQFFPDVVQFSEDLANQ-GKTVII 124

Query: 145 AGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTELIAGADVY 204
           A LDG + R+ F SV+D+V  A+ +TKLTA C +C   A F+ R  E    ELI G D Y
Sbjct: 125 AALDGTFQRKPFQSVIDLVSKAEYITKLTAVCMVCYNEAAFSKRIVESDDIELIGGIDKY 184

Query: 205 MPVCRHHYANGQEVIQSSK 217
           + VCR  Y + Q    S+K
Sbjct: 185 ISVCRGCYNSDQNEGNSTK 194

BLAST of Carg16093 vs. TrEMBL
Match: tr|A0A1S3CKW8|A0A1S3CKW8_CUCME (Thymidine kinase OS=Cucumis melo OX=3656 GN=LOC103502126 PE=3 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 4.1e-111
Identity = 203/237 (85.65%), Postives = 219/237 (92.41%), Query Frame = 0

Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           MES R+S SLTS++  A+   S HR+ GEVHVIIGPMFAGKTTALLRRIK+ S+SGRNVV
Sbjct: 1   MESCRSSLSLTSSNADAIRPESSHRLTGEVHVIIGPMFAGKTTALLRRIKSASNSGRNVV 60

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKDTRYA+DSVVTHDGAKFPCWALP LSSFRQKFGEDAYN+LDVIGVDEAQFFDDL
Sbjct: 61  MIKSSKDTRYAVDSVVTHDGAKFPCWALPHLSSFRQKFGEDAYNELDVIGVDEAQFFDDL 120

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCC+VAD+D KII+VAGLDGDYLRR FGSVLDVVPLADTVTKLTARCELCGKRAFFTL
Sbjct: 121 YDFCCNVADRDGKIIVVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 238
           RKTEET+TELIAGADVYMPVCRHHYA GQEVIQSSKN++GSR +   SNLEALPSLI
Sbjct: 181 RKTEETKTELIAGADVYMPVCRHHYATGQEVIQSSKNVMGSRTI--ASNLEALPSLI 235

BLAST of Carg16093 vs. TrEMBL
Match: tr|A0A0A0KAS7|A0A0A0KAS7_CUCSA (Thymidine kinase OS=Cucumis sativus OX=3659 GN=Csa_6G110310 PE=3 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 9.4e-108
Identity = 200/233 (85.84%), Postives = 211/233 (90.56%), Query Frame = 0

Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           MES R S   TS+    V   S HR+AGEVHVIIGPMFAGKTTALLRRIK+ES+SGRNVV
Sbjct: 1   MESCRTSLPFTSSYADTVRPESSHRLAGEVHVIIGPMFAGKTTALLRRIKSESNSGRNVV 60

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKDTRYAIDSVVTHDG KFPCWALPDLSSFRQKFGEDAYN+LDVIGVDEAQFFDDL
Sbjct: 61  MIKSSKDTRYAIDSVVTHDGVKFPCWALPDLSSFRQKFGEDAYNELDVIGVDEAQFFDDL 120

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCC+VAD+D KII+VAGLDGDYLRR FGSVLDVVPLADTVTKLTARCELCGKRAFFTL
Sbjct: 121 YDFCCNVADRDGKIIVVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEAL 234
           RKTEET+TELIAGADVYMPVCRHHYA GQEVIQSSKN +GSR +   SNLEAL
Sbjct: 181 RKTEETKTELIAGADVYMPVCRHHYAIGQEVIQSSKNAMGSRTI--ASNLEAL 231

BLAST of Carg16093 vs. TrEMBL
Match: tr|F6HHL3|F6HHL3_VITVI (Thymidine kinase OS=Vitis vinifera OX=29760 GN=VIT_12s0057g00500 PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 9.1e-95
Identity = 171/222 (77.03%), Postives = 192/222 (86.49%), Query Frame = 0

Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           M S ++  SLTST G     GS HR  GEVHVI+GPMFAGKTTALLRRIK+ES++GRNV 
Sbjct: 1   MSSFKSPISLTSTGG----VGSIHRALGEVHVIVGPMFAGKTTALLRRIKSESNNGRNVA 60

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKDTRYA DS+VTHDG KFPCWALPDLSSFRQKFG +AY KL+VIG+DEAQFF+DL
Sbjct: 61  MIKSSKDTRYATDSIVTHDGVKFPCWALPDLSSFRQKFGAEAYEKLEVIGIDEAQFFEDL 120

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCC+VAD D K +IVAGLDGDYLRR FGSVLDV+PLAD+VTKLTARCELCGKRAFFTL
Sbjct: 121 YDFCCEVADHDGKTVIVAGLDGDYLRRSFGSVLDVIPLADSVTKLTARCELCGKRAFFTL 180

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSR 223
           RK EE +TELI GAD+YMPVCR HY NGQ VI++++N L SR
Sbjct: 181 RKIEEMQTELIGGADIYMPVCRQHYVNGQAVIEAARNALESR 218

BLAST of Carg16093 vs. TrEMBL
Match: tr|V4W4L4|V4W4L4_9ROSI (Thymidine kinase OS=Citrus clementina OX=85681 GN=CICLE_v10016471mg PE=3 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 4.2e-92
Identity = 163/224 (72.77%), Postives = 192/224 (85.71%), Query Frame = 0

Query: 14  DGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVVMIKSSKDTRYAID 73
           + G    G PHR +GE+HVI+GPMFAGKTTALLRRI++ES +GRN+ MIKSSKDTRYAID
Sbjct: 17  NSGRDRVGLPHRNSGEIHVIMGPMFAGKTTALLRRIRSESDNGRNIAMIKSSKDTRYAID 76

Query: 74  SVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDLYDFCCDVADKDDK 133
           SVVTHDGAK PCWALP+L+SFRQK G+DAY KLDVIG+DEAQFF+DLY+FCC+ AD D K
Sbjct: 77  SVVTHDGAKLPCWALPELTSFRQKLGDDAYAKLDVIGIDEAQFFEDLYEFCCNAADHDGK 136

Query: 134 IIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTELIAG 193
            ++VAGLDGDYLRR FGSVLD++PLADTVTKLTARCELCGKRAFFTLRKTEET TE+I G
Sbjct: 137 TVVVAGLDGDYLRRSFGSVLDIIPLADTVTKLTARCELCGKRAFFTLRKTEETETEIIGG 196

Query: 194 ADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 238
           ADVYMPVCR HY NGQ VI +++N+L    +++ S +EA  + I
Sbjct: 197 ADVYMPVCRQHYVNGQVVIGAARNVLEFHKVQSKSCVEAAAAAI 240

BLAST of Carg16093 vs. TrEMBL
Match: tr|A0A2P6S1K0|A0A2P6S1K0_ROSCH (Thymidine kinase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2g0156811 PE=3 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 4.2e-92
Identity = 170/232 (73.28%), Postives = 195/232 (84.05%), Query Frame = 0

Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           M S + +  LTS      SA S    +GEVHVI+GPMFAGKTTALLRRIK+E ++GRNV 
Sbjct: 28  MASFKPTIPLTSE-----SAPSRRPPSGEVHVIMGPMFAGKTTALLRRIKSEGANGRNVA 87

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKD RYA+DSVVTHDGAKFPCWALPDLSSFR KFG DAY+KLDVIG+DEAQFF+DL
Sbjct: 88  MIKSSKDNRYAVDSVVTHDGAKFPCWALPDLSSFRHKFGGDAYDKLDVIGIDEAQFFEDL 147

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCC  AD D K +IVAGLDGDYLR+ FGSVLDVVPLADTVTKLTARCE+CGKRAFFTL
Sbjct: 148 YDFCCTAADHDGKTVIVAGLDGDYLRKSFGSVLDVVPLADTVTKLTARCEMCGKRAFFTL 207

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEA 233
           RKTEET+TELI GADVYMPVCR HY NGQ +I+S+++++ S   ++G+  EA
Sbjct: 208 RKTEETKTELIGGADVYMPVCRQHYVNGQVLIKSARSVVESHKFKSGTISEA 254

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023513380.12.9e-12999.58thymidine kinase a [Cucurbita pepo subsp. pepo][more]
XP_022943895.11.1e-12899.16thymidine kinase a [Cucurbita moschata][more]
XP_022985744.15.2e-12697.89thymidine kinase-like [Cucurbita maxima][more]
XP_022140502.13.7e-11689.83thymidine kinase [Momordica charantia][more]
XP_008464186.16.1e-11185.65PREDICTED: thymidine kinase [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT3G07800.12.7e-8468.02Thymidine kinase[more]
AT5G23070.18.9e-8063.68Thymidine kinase[more]
Match NameE-valueIdentityDescription
sp|Q9S750|KITHA_ARATH4.8e-8368.02Thymidine kinase a OS=Arabidopsis thaliana OX=3702 GN=TK1A PE=1 SV=1[more]
sp|O81263|KITH_ORYSJ6.3e-8372.54Thymidine kinase OS=Oryza sativa subsp. japonica OX=39947 GN=TK PE=2 SV=2[more]
sp|F4KBF5|KITHB_ARATH1.6e-7863.68Thymidine kinase b OS=Arabidopsis thaliana OX=3702 GN=TK1B PE=1 SV=1[more]
sp|P23335|KITH_SWPVK4.1e-3441.57Thymidine kinase OS=Swinepox virus (strain Kasza) OX=10277 GN=TK PE=3 SV=1[more]
sp|Q27564|KITH_DICDI9.2e-3443.72Thymidine kinase 1 OS=Dictyostelium discoideum OX=44689 GN=thyB PE=1 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A1S3CKW8|A0A1S3CKW8_CUCME4.1e-11185.65Thymidine kinase OS=Cucumis melo OX=3656 GN=LOC103502126 PE=3 SV=1[more]
tr|A0A0A0KAS7|A0A0A0KAS7_CUCSA9.4e-10885.84Thymidine kinase OS=Cucumis sativus OX=3659 GN=Csa_6G110310 PE=3 SV=1[more]
tr|F6HHL3|F6HHL3_VITVI9.1e-9577.03Thymidine kinase OS=Vitis vinifera OX=29760 GN=VIT_12s0057g00500 PE=3 SV=1[more]
tr|V4W4L4|V4W4L4_9ROSI4.2e-9272.77Thymidine kinase OS=Citrus clementina OX=85681 GN=CICLE_v10016471mg PE=3 SV=1[more]
tr|A0A2P6S1K0|A0A2P6S1K0_ROSCH4.2e-9273.28Thymidine kinase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2g0156811 PE=3 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004797thymidine kinase activity
GO:0005524ATP binding
Vocabulary: INTERPRO
TermDefinition
IPR027417P-loop_NTPase
IPR020633Thymidine_kinase_CS
IPR001267Thymidine_kinase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006206 pyrimidine nucleobase metabolic process
biological_process GO:0006230 TMP biosynthetic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0004797 thymidine kinase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg16093-RACarg16093-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.30.60.20coord: 166..207
e-value: 7.5E-15
score: 56.9
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 26..165
e-value: 1.1E-47
score: 163.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..18
NoneNo IPR availablePANTHERPTHR11441:SF2THYMIDINE KINASE Bcoord: 17..228
NoneNo IPR availableSUPERFAMILYSSF57716Glucocorticoid receptor-like (DNA-binding domain)coord: 166..208
IPR001267Thymidine kinasePFAMPF00265TKcoord: 28..205
e-value: 1.0E-52
score: 178.5
IPR001267Thymidine kinasePIRSFPIRSF035805Tdkcoord: 25..218
e-value: 6.5E-61
score: 203.0
IPR001267Thymidine kinasePANTHERPTHR11441THYMIDINE KINASEcoord: 17..228
IPR020633Thymidine kinase, conserved sitePROSITEPS00603TK_CELLULAR_TYPEcoord: 192..205
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 28..166

The following gene(s) are paralogous to this gene:

None