Carg10035 (gene) Silver-seed gourd

NameCarg10035
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionHomocysteine S-methyltransferase
LocationCucurbita_argyrosperma_scaffold_039 : 36512 .. 40357 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGTATTCTCGCTCGTCTTTCGCTAACAGTCGGAGCAATGAACGAGGCACGTGTTCCGGCGGAAACCACCACCTTCATGACTGATTTTCTGAGACAGTGCGGCGGCTACGGCGTTATAGACGGCGGCTTGGCCACTGAACTGGAACGGCATGGCGCCGATCTTAACGATCCTCTCTGGAGCGCTAAATGCCTCGTCAGTTCGCCTCACATCGTCCGACGGGTACTTTCTTTTATAAATTCTGGGCATAATAAAATTTTAAATTTATTCTTTTTTTTATTTTTAATAAAAAGGTAGGAATTTATGTGATGTAAAATAAAAAAATTAAATATATGTATTGGATATAAATTAAAAAATTTAGGATTATCTAATATTTAATAAACGGTTAATTGATATATCGTTGAATTTAAATTAAGTTATATATTAGACTATTAAAATGAGAATAACTTAATTTTATTTATTTATTCTTTGCAATCTTATTTTATTTTATTTTTTCTACTAAAGTTCTCATAATTATAAAAAGTTGCTGTAGATCACTAAATGTTTTTTTTTTTTTTTTGAAAAATAGAGAATAAAAACAGAATAATTATCGCATTGAGCTGTATTTTTTTTCTTTTTTTAAATCACGATTTGCAGATTTAAATAAATTATAATTTAATTTGTCGGTTGTACATCGTTCTGGGCCGGAGAATGAATGGGCCGTCGTAGGCCCAATATGAAAATAGGGCTCATTTAACCAACAGGTCACTTGTTCGAACCGTATCCGCGCCACCTTTTGAAATTAATTAATTTTTTGGTTTGACTTTTTAAAATATTTAATGGACATTTAATTTTTTTATAAAAATTATTTTTGTCTAAATTACAGAAACAACAGACTCTATGTTATTCGGTTATTTCCAAGAGGCCTCGCATCAATGAACATGTATTCGTTACTAATAAACCCATAATCATTTCTTAAATTAGTTAATGTGGGACTCCCTCCCAACGAACAACCGTGATGAGCTAAGCTAATCGGCTTAATATGACAGGTACATTTAGACTACCTTGATGCTGGTGCCAATATCATTACCACAGCATCTTATCAGGTTCATTTCTTCACCTCATTTCACTGTTTCATAGCTTCTTATTGTTTAAATTTTTGCTACAAATGATCTTGAATACTTGTTTGGTTATTGCTCTAGATTCAAAATGTTAAGAAACCCATGATTCCATTGTGTTTGCAGGCAACAATCCAGGGTTTTGAGGCTAAGGGATTTTCTAGAGATGAAAGTGAAGAATTGCTAAGAAAAAGTGTTGAAATTGCTCTTGAAGCTCGTGAGATCTATCTCGAACGATGTACAAAGGATTCTTGGGATTGGACTGCAAGTGAAAGGGGCTCGACGAGGCCGATTCTTGTAGCCGCCTCGGTCGGCAGCTACGGAGCTTATTTGGCTGATGGGTCTGAATACAGGTGAGCAAAATCTCGGCATAGTCTTAGAATGGACTTCAATGGTTGCAAATTTGAGCTGTGGTTGTACCTTTTTACAGTGGGAAGTATGGCGATTCAGTTAGTTTAGAAACACTGAAAGATTTTCATAGGAGAAGGGTGCAGATTTTGGCCAATGCTGGTGCTGATTTAATCGCGTTCGAGACGATTCCGAACAAGTTGGAAGCTCAAGTATGAAAATGGTGTTGGCAGGGTTCCTCCTTGTTTAAGTTCTTTATGCTGTTTATGTGGAATTTGCAGGCATATGCTGAGCTTCTTGATGAAGAGGGCATTGAAATTCCTGCTTGGTTTTCTTTCAACTCCAAGGATGGTATCAATGTGGTGAGTGGGGATTCTATTACTGATTGTGCCTCCATTGCTGATGCTTCGAAGCGAGTTGTTGCTGTGGGAATCAACTGTACTCCTCCCAGATATATTCATGGACTAATCTCGTCGATTCGGGAGGTAAATTCCTCGAATACCCTTTTATGACTTCGGAGTTTGCAAGAGTTGTTCTACGTTCAAGTTGGCATAACTTTAGTGTAAATTTGAGGATTTGGGAATGAGTGTGTGCACAAATGAAGTCATTTCAATCTCTCGTAACAAGATTTATCAACCAAGTTTTCCATCTGCCTCTGTACCATGAAAGATGAATAGTATTCATGTAATAGCCCACTGCCGGTAGATATTGTCTTCTTTGAGCTTTTACTTTTGGACTTCCCCTCAAGGTTTTAAAACGCGTCTATTAGGTAGAGGTTTCGACACTCTTATAAGGAAGTACAACTGACGTGGGATTTCAGAATCCACCCCCTTGGGGGACCCAGTGTCCTCACTGGCACACCACCCGATATTTAGAACTGATACTATTTGTAATACCCCACCAATAGCAAATATTGTCCTTTTTGGGCTTTCCCTTTTGGACTTCCCCTTGGAGGACCCAACGTCCTCGCTGGCACACCGCTCGGTGTCTAGCTCTGATACTGGTTGTAACAGCTCACTGCTAGTAGATATTATCCTCTTCGAGCTTTCCCTTCTAAACTTCTCCTTAAGGTTCTAAAACGTGTCTACTAGGGAAAAGTTTCCACACCCTTATAAGGAATGTTTCGTTCCCCTCTCCAAACAATGTGGGATCTCACAATCCACCCCCTTGGGGGTGCCAGCGTTTCGCTGGCACACAGCCCGGTATCTAGCTCTAATACCATTTGTAACAGCCCACCGCTATTAGATATTGTCCTCTTTAAGTTTTCCCTTCTAAACTTCATTTCAAGGTTTTAAAACGCGTCTACTAGGGAAAAGTTTCCACACCCATATAAGGAATGTTTCATTCCACTCTCTAATTGATGTGGGATCTCACAATCCACTCCCTTGGCGGACCCAACGTCCTCGCTGGCACACCACCCGGTGTCTGACTCTTATACCATTTGTAACAGTCCACCGCTAGCATATATTGTCCTCTTTGGGCTTTCCCTTCTAAACTTCCCCTCAAGGTTTTAAAACGCGTCTACTAGGGAGAGGTTTCCACACCCTTATGAAGAATACTTCGTTCCCCTCTCAAACCGACGTAGGATCTCACAATTCATGTTCCTTAAAATAGGAAAACACTAAGCCATAGCCACCTTTCTTCACTACCAAAGGCTTGTGCTAAGCCCCAGTCATAATAATGGACACAAGAGCAGCAGGAACAACATGGAAAGCAGTCTAATAATGGAAAAAGAACACTGAAAGCAGCCCTCAAATATGTTCATATTCTAAGTAAATCTTGTTCTCTAATTGGCAGGTCACTAACAAACCCATTCTGATTTATCCAAACAGCGGGGAGACATATGATGGTGAAACCAAACAGTGGTTGGTGAGTGTCCTACTACTTTTCCTGTGTGTGTTCTTTGAACTTCACGGTTTCGTTCCTTCGTATACCACGACGGTCAGGTTCCCCCGTCCTCAATCACTTCTTTTATCTTTGCAGAAATCTGATGGAATGACAGGGGAAGACTTCGTGTCATATGTAAGTAAGTGGAAGGAAGCAGGAGCCTCTCTGTTTGGCGGATGCTGCCGCACCACTCCAAACACAATCCAAGGCATAGCAAAATCGCTTTCTTCTAAAGCCTTTTGAGCCGTTTCCCCTAATTAACAAGCTTGGTATGAACTATATCCAATATATCAGCTCTGTTTTAGATTATGGGATGAACATAAATTTTTGTAAATATTAAGGAAATGAGAGCTGTTCAAGAAATAAGTTGTTCATGTTCAAAGAATACTTGATTTGACTTGGGATGATAAATGATGAAGTTCCCACAAGCGTTTTCATGATGCAATAATTTGAAATTGATAGTGATTGATGGTTTCATTGCTAGCCACCAATTGCAAGTCCTAAGCCCAGAAAG

mRNA sequence

ATGCGTATTCTCGCTCGTCTTTCGCTAACAGTCGGAGCAATGAACGAGGCACGTGTTCCGGCGGAAACCACCACCTTCATGACTGATTTTCTGAGACAGTGCGGCGGCTACGGCGTTATAGACGGCGGCTTGGCCACTGAACTGGAACGGCATGGCGCCGATCTTAACGATCCTCTCTGGAGCGCTAAATGCCTCGTCAGTTCGCCTCACATCGTCCGACGGGTACATTTAGACTACCTTGATGCTGGTGCCAATATCATTACCACAGCATCTTATCAGGCAACAATCCAGGGTTTTGAGGCTAAGGGATTTTCTAGAGATGAAAGTGAAGAATTGCTAAGAAAAAGTGTTGAAATTGCTCTTGAAGCTCGTGAGATCTATCTCGAACGATGTACAAAGGATTCTTGGGATTGGACTGCAAGTGAAAGGGGCTCGACGAGGCCGATTCTTGTAGCCGCCTCGGTCGGCAGCTACGGAGCTTATTTGGCTGATGGGTCTGAATACAGTGGGAAGTATGGCGATTCAGTTAGTTTAGAAACACTGAAAGATTTTCATAGGAGAAGGGTGCAGATTTTGGCCAATGCTGGTGCTGATTTAATCGCGTTCGAGACGATTCCGAACAAGTTGGAAGCTCAAGCATATGCTGAGCTTCTTGATGAAGAGGGCATTGAAATTCCTGCTTGGTTTTCTTTCAACTCCAAGGATGGTATCAATGTGGTGAGTGGGGATTCTATTACTGATTGTGCCTCCATTGCTGATGCTTCGAAGCGAGTTGTTGCTGTGGGAATCAACTGTACTCCTCCCAGATATATTCATGGACTAATCTCGTCGATTCGGGAGGTCACTAACAAACCCATTCTGATTTATCCAAACAGCGGGGAGACATATGATGGTGAAACCAAACAGTGGTTGAAATCTGATGGAATGACAGGGGAAGACTTCGTGTCATATGTAAGTAAGTGGAAGGAAGCAGGAGCCTCTCTGTTTGGCGGATGCTGCCGCACCACTCCAAACACAATCCAAGGCATAGCAAAATCGCTTTCTTCTAAAGCCTTTTGAGCCGTTTCCCCTAATTAACAAGCTTGGTATGAACTATATCCAATATATCAGCTCTGTTTTAGATTATGGGATGAACATAAATTTTTGTAAATATTAAGGAAATGAGAGCTGTTCAAGAAATAAGTTGTTCATGTTCAAAGAATACTTGATTTGACTTGGGATGATAAATGATGAAGTTCCCACAAGCGTTTTCATGATGCAATAATTTGAAATTGATAGTGATTGATGGTTTCATTGCTAGCCACCAATTGCAAGTCCTAAGCCCAGAAAG

Coding sequence (CDS)

ATGCGTATTCTCGCTCGTCTTTCGCTAACAGTCGGAGCAATGAACGAGGCACGTGTTCCGGCGGAAACCACCACCTTCATGACTGATTTTCTGAGACAGTGCGGCGGCTACGGCGTTATAGACGGCGGCTTGGCCACTGAACTGGAACGGCATGGCGCCGATCTTAACGATCCTCTCTGGAGCGCTAAATGCCTCGTCAGTTCGCCTCACATCGTCCGACGGGTACATTTAGACTACCTTGATGCTGGTGCCAATATCATTACCACAGCATCTTATCAGGCAACAATCCAGGGTTTTGAGGCTAAGGGATTTTCTAGAGATGAAAGTGAAGAATTGCTAAGAAAAAGTGTTGAAATTGCTCTTGAAGCTCGTGAGATCTATCTCGAACGATGTACAAAGGATTCTTGGGATTGGACTGCAAGTGAAAGGGGCTCGACGAGGCCGATTCTTGTAGCCGCCTCGGTCGGCAGCTACGGAGCTTATTTGGCTGATGGGTCTGAATACAGTGGGAAGTATGGCGATTCAGTTAGTTTAGAAACACTGAAAGATTTTCATAGGAGAAGGGTGCAGATTTTGGCCAATGCTGGTGCTGATTTAATCGCGTTCGAGACGATTCCGAACAAGTTGGAAGCTCAAGCATATGCTGAGCTTCTTGATGAAGAGGGCATTGAAATTCCTGCTTGGTTTTCTTTCAACTCCAAGGATGGTATCAATGTGGTGAGTGGGGATTCTATTACTGATTGTGCCTCCATTGCTGATGCTTCGAAGCGAGTTGTTGCTGTGGGAATCAACTGTACTCCTCCCAGATATATTCATGGACTAATCTCGTCGATTCGGGAGGTCACTAACAAACCCATTCTGATTTATCCAAACAGCGGGGAGACATATGATGGTGAAACCAAACAGTGGTTGAAATCTGATGGAATGACAGGGGAAGACTTCGTGTCATATGTAAGTAAGTGGAAGGAAGCAGGAGCCTCTCTGTTTGGCGGATGCTGCCGCACCACTCCAAACACAATCCAAGGCATAGCAAAATCGCTTTCTTCTAAAGCCTTTTGA

Protein sequence

MRILARLSLTVGAMNEARVPAETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHLDYLDAGANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTKDSWDWTASERGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGADLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIADASKRVVAVGINCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGEDFVSYVSKWKEAGASLFGGCCRTTPNTIQGIAKSLSSKAF
BLAST of Carg10035 vs. NCBI nr
Match: XP_022959458.1 (homocysteine S-methyltransferase 3-like [Cucurbita moschata] >XP_022959459.1 homocysteine S-methyltransferase 3-like [Cucurbita moschata])

HSP 1 Score: 703.0 bits (1813), Expect = 5.2e-199
Identity = 348/352 (98.86%), Postives = 352/352 (100.00%), Query Frame = 0

Query: 1   MRILARLSLTVGAMNEARVPAETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLW 60
           MRIL+RLSLTVGAMNEARVPAETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLW
Sbjct: 1   MRILSRLSLTVGAMNEARVPAETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLW 60

Query: 61  SAKCLVSSPHIVRRVHLDYLDAGANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIA 120
           SAKCLVSSPHIVRRVHLDYLDAGANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIA
Sbjct: 61  SAKCLVSSPHIVRRVHLDYLDAGANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIA 120

Query: 121 LEAREIYLERCTKDSWDWTASERGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLET 180
           LEAREIYLERCTKDSWDWTA+ERGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLET
Sbjct: 121 LEAREIYLERCTKDSWDWTATERGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLET 180

Query: 181 LKDFHRRRVQILANAGADLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVV 240
           LKDFHRRRVQILANAGADLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGIN+V
Sbjct: 181 LKDFHRRRVQILANAGADLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINMV 240

Query: 241 SGDSITDCASIADASKRVVAVGINCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGET 300
           SGDSITDCASIADASKRVVAVGINCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGET
Sbjct: 241 SGDSITDCASIADASKRVVAVGINCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGET 300

Query: 301 KQWLKSDGMTGEDFVSYVSKWKEAGASLFGGCCRTTPNTIQGIAKSLSSKAF 353
           KQWLKSDGMTGEDFVSYVSKWKEAGASLFGGCCRTTPNTIQGIAK+LSSKAF
Sbjct: 301 KQWLKSDGMTGEDFVSYVSKWKEAGASLFGGCCRTTPNTIQGIAKALSSKAF 352

BLAST of Carg10035 vs. NCBI nr
Match: XP_023549248.1 (homocysteine S-methyltransferase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 682.9 bits (1761), Expect = 5.6e-193
Identity = 336/339 (99.12%), Postives = 339/339 (100.00%), Query Frame = 0

Query: 14  MNEARVPAETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVR 73
           MNEARVPAETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVR
Sbjct: 1   MNEARVPAETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVR 60

Query: 74  RVHLDYLDAGANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTK 133
           RVHLDYLDAGANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTK
Sbjct: 61  RVHLDYLDAGANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTK 120

Query: 134 DSWDWTASERGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILA 193
           DSWDWTA+ERGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILA
Sbjct: 121 DSWDWTATERGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILA 180

Query: 194 NAGADLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIAD 253
           NAGADLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIAD
Sbjct: 181 NAGADLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIAD 240

Query: 254 ASKRVVAVGINCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGED 313
           ASKRVVAVGINCTPPRYIHGLISSIREVTNKPI+IYPNSGETYDGETKQWLKSDGMTGED
Sbjct: 241 ASKRVVAVGINCTPPRYIHGLISSIREVTNKPIVIYPNSGETYDGETKQWLKSDGMTGED 300

Query: 314 FVSYVSKWKEAGASLFGGCCRTTPNTIQGIAKSLSSKAF 353
           FVSYVSKWKEAGASLFGGCCRTTPNTIQGIAK+LSSKAF
Sbjct: 301 FVSYVSKWKEAGASLFGGCCRTTPNTIQGIAKALSSKAF 339

BLAST of Carg10035 vs. NCBI nr
Match: XP_023006735.1 (homocysteine S-methyltransferase 3-like [Cucurbita maxima] >XP_023006736.1 homocysteine S-methyltransferase 3-like [Cucurbita maxima])

HSP 1 Score: 667.2 bits (1720), Expect = 3.2e-188
Identity = 329/339 (97.05%), Postives = 334/339 (98.53%), Query Frame = 0

Query: 14  MNEARVPAETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVR 73
           MNEARVPAETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVR
Sbjct: 1   MNEARVPAETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVR 60

Query: 74  RVHLDYLDAGANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTK 133
           RVHLDYLDAGANIITTASYQATI GFEAKGFSRDESEELLRKSVEIALEAREIYLERCTK
Sbjct: 61  RVHLDYLDAGANIITTASYQATILGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTK 120

Query: 134 DSWDWTASERGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILA 193
           DSWDWTASER STR ILVAASVGSYGAYLADGSEYSGKYGDSV+LETLKDFHRRRVQILA
Sbjct: 121 DSWDWTASERSSTRLILVAASVGSYGAYLADGSEYSGKYGDSVTLETLKDFHRRRVQILA 180

Query: 194 NAGADLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIAD 253
           NAGADLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGIN VSGDSITDCASIAD
Sbjct: 181 NAGADLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINAVSGDSITDCASIAD 240

Query: 254 ASKRVVAVGINCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGED 313
           ASK+VVAVGINCTPPRYIHGLIS IREVTNKPI+IYPNSGETYDGETKQWL+SDGMTGED
Sbjct: 241 ASKQVVAVGINCTPPRYIHGLISLIREVTNKPIVIYPNSGETYDGETKQWLQSDGMTGED 300

Query: 314 FVSYVSKWKEAGASLFGGCCRTTPNTIQGIAKSLSSKAF 353
           FVSYVSKWKEAGASLFGGCCRTTPNTIQGIAK+LSSKAF
Sbjct: 301 FVSYVSKWKEAGASLFGGCCRTTPNTIQGIAKALSSKAF 339

BLAST of Carg10035 vs. NCBI nr
Match: XP_008449335.1 (PREDICTED: homocysteine S-methyltransferase 3 [Cucumis melo])

HSP 1 Score: 602.1 bits (1551), Expect = 1.3e-168
Identity = 287/335 (85.67%), Postives = 320/335 (95.52%), Query Frame = 0

Query: 18  RVPAETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHL 77
           ++P   TT++T FL++CGGYGV+DGG ATELER GADLNDPLWSAKCL++SPH+VRRVHL
Sbjct: 4   QMPETATTYLTHFLQKCGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHL 63

Query: 78  DYLDAGANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTKDSWD 137
           DYLDAGANII+TASYQAT+QGFEAKGFSRDESE+LLRKSV+IA+EAR+IYLERCTKDSWD
Sbjct: 64  DYLDAGANIISTASYQATLQGFEAKGFSRDESEDLLRKSVQIAIEARDIYLERCTKDSWD 123

Query: 138 WTASERGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGA 197
           +T +  GS RP+L+AAS+GSYGAYLADGSEYSG+YG+SV+LETLK+FHRRRVQILANAGA
Sbjct: 124 FTETGAGSRRPVLIAASIGSYGAYLADGSEYSGEYGESVTLETLKNFHRRRVQILANAGA 183

Query: 198 DLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIADASKR 257
           DLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSI+DCASIADASK+
Sbjct: 184 DLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQ 243

Query: 258 VVAVGINCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGEDFVSY 317
           VVAVGINCTPPRYIHGLI SI+EVT+KPI++YPNSGETYDG TKQWLKSDGM GEDFVSY
Sbjct: 244 VVAVGINCTPPRYIHGLILSIQEVTDKPIVVYPNSGETYDGVTKQWLKSDGMIGEDFVSY 303

Query: 318 VSKWKEAGASLFGGCCRTTPNTIQGIAKSLSSKAF 353
           V KWKEAGASLFGGCCRTTPNTI+GIAK+LSSK F
Sbjct: 304 VRKWKEAGASLFGGCCRTTPNTIRGIAKALSSKTF 338

BLAST of Carg10035 vs. NCBI nr
Match: XP_004145385.1 (PREDICTED: homocysteine S-methyltransferase 3 [Cucumis sativus] >KGN61674.1 hypothetical protein Csa_2G223160 [Cucumis sativus])

HSP 1 Score: 601.3 bits (1549), Expect = 2.1e-168
Identity = 290/335 (86.57%), Postives = 317/335 (94.63%), Query Frame = 0

Query: 18  RVPAETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHL 77
           ++P   TTF+T FL+  GGYGV+DGG ATELER GADLNDPLWSAKCL++SPH+VRRVHL
Sbjct: 4   QMPETATTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHL 63

Query: 78  DYLDAGANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTKDSWD 137
           DYLDAGANII+TASYQATIQGFEAKGFSRDESE LLRKSV+IA+EAR+IYLERCTKDSWD
Sbjct: 64  DYLDAGANIISTASYQATIQGFEAKGFSRDESENLLRKSVQIAIEARDIYLERCTKDSWD 123

Query: 138 WTASERGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGA 197
           +T +  GS RPIL+AAS+GSYGAYLADGSEYSG+YGDSV+LETLKDFHRRRVQILANAGA
Sbjct: 124 FTETGAGSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGA 183

Query: 198 DLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIADASKR 257
           DLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSI+DCASIADASK+
Sbjct: 184 DLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQ 243

Query: 258 VVAVGINCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGEDFVSY 317
           VVAVGINCTPPRYIHGLI SIREVT+KPI++YPNSGE+YDG TK+WLKSDGM GEDFVSY
Sbjct: 244 VVAVGINCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSY 303

Query: 318 VSKWKEAGASLFGGCCRTTPNTIQGIAKSLSSKAF 353
           V KWKEAGASLFGGCCRTTPNTI+GIAK+LSSK F
Sbjct: 304 VRKWKEAGASLFGGCCRTTPNTIRGIAKALSSKTF 338

BLAST of Carg10035 vs. TAIR10
Match: AT3G22740.1 (homocysteine S-methyltransferase 3)

HSP 1 Score: 516.5 bits (1329), Expect = 1.3e-146
Identity = 243/332 (73.19%), Postives = 286/332 (86.14%), Query Frame = 0

Query: 19  VPAETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHLD 78
           V  ET++ MTDFL +CGGY V+DGG ATEL+RHGAD+NDPLWSAKCL++SPH+V +VHLD
Sbjct: 5   VKEETSSLMTDFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTKVHLD 64

Query: 79  YLDAGANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTKDSWDW 138
           YL++GANII TASYQATIQGF AKG S  E+E LLR+SVEI  EAREI+  RCTK SWD+
Sbjct: 65  YLESGANIIITASYQATIQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDF 124

Query: 139 TASERGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGAD 198
             + + S RPILVAASVGSYGAYLADGSEYSG YGDSVS ETLKDFHRRRVQILA +GAD
Sbjct: 125 AYAGKASRRPILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGAD 184

Query: 199 LIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIADASKRV 258
           LIAFETIPNKLEA+AYA+LL+EE I+IPAWFSF SKDG++V  GDS+ +CA +AD+ K V
Sbjct: 185 LIAFETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVADSCKNV 244

Query: 259 VAVGINCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGEDFVSYV 318
           VA+GINCT PRYIH LI S+R++T KPI++YPNSGE YDG  K+W+KS+G + EDFVSYV
Sbjct: 245 VAIGINCTAPRYIHALIISLRQMTRKPIVVYPNSGEVYDGLNKKWIKSEGESEEDFVSYV 304

Query: 319 SKWKEAGASLFGGCCRTTPNTIQGIAKSLSSK 351
           SKW++AGASLFGGCCRTTPNTI+ IAK LS +
Sbjct: 305 SKWRDAGASLFGGCCRTTPNTIRAIAKVLSDE 336

BLAST of Carg10035 vs. TAIR10
Match: AT3G63250.1 (homocysteine methyltransferase 2)

HSP 1 Score: 476.5 bits (1225), Expect = 1.4e-134
Identity = 229/325 (70.46%), Postives = 275/325 (84.62%), Query Frame = 0

Query: 27  MTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHLDYLDAGANI 86
           M DFL+Q GGY VIDGGLATE ERHGADLNDPLWSAKCLV+SPH++  VHLDYL+AGA+I
Sbjct: 9   MKDFLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAGADI 68

Query: 87  ITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERC-TKDSWDWTASERGS 146
           I++ASYQATIQGFEAKGFSR+ESE LL+KSVEIA EAR  Y ++C T  S D    ++  
Sbjct: 69  ISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMDDKILKK-- 128

Query: 147 TRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGADLIAFETI 206
            RPILVAASVGSYGAYLADGSEYSG YGDS++LE LKDFHRRR+Q+LA +GADLIAFETI
Sbjct: 129 -RPILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETI 188

Query: 207 PNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIADASKRVVAVGINC 266
           PNK+EAQA+A+LL+E  ++IP WFSFNSKDG+NVVSGDSI +C SIA+  ++VVAVGINC
Sbjct: 189 PNKIEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINC 248

Query: 267 TPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGEDFVSYVSKWKEAG 326
           TPPR+I GL+  I +VT+KPIL+YPNSGE+YD + K+W+++ G+  EDFVSYV KW +AG
Sbjct: 249 TPPRFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENTGVGDEDFVSYVEKWMDAG 308

Query: 327 ASLFGGCCRTTPNTIQGIAKSLSSK 351
            SL GGCCRTTP TI+ I K L ++
Sbjct: 309 VSLLGGCCRTTPTTIRAIHKRLVNR 330

BLAST of Carg10035 vs. TAIR10
Match: AT3G25900.1 (Homocysteine S-methyltransferase family protein)

HSP 1 Score: 374.8 bits (961), Expect = 5.9e-104
Identity = 176/327 (53.82%), Postives = 237/327 (72.48%), Query Frame = 0

Query: 24  TTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHLDYLDAG 83
           +  + D +++CGG  V+DGG AT+LE HGA +NDPLWSA  L+ +P +++RVH++YL+AG
Sbjct: 7   SALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHMEYLEAG 66

Query: 84  ANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTKDSWDWTASER 143
           A+I+ T+SYQATI GF ++G S +ESE LL+KSVE+A+EAR+ + E+ +K S        
Sbjct: 67  ADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH------ 126

Query: 144 GSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGADLIAFE 203
            S    LVAAS+GSYGAYLADGSEYSG YG++VSL+ LKDFHRRR+Q+L  AG DL+AFE
Sbjct: 127 -SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFE 186

Query: 204 TIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIADASKRVVAVGI 263
           TIPNKLEAQA  ELL+EE ++IPAW  F S DG    SG+S  +C    + S  + AVGI
Sbjct: 187 TIPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGI 246

Query: 264 NCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGEDFVSYVSKWKE 323
           NC PP++I  LI    ++T K I++YPNSGE +DG+ KQWL S     ++F  + +KW++
Sbjct: 247 NCAPPQFIENLIRKFAKLTKKAIVVYPNSGEVWDGKAKQWLPSQCFGDDEFEMFATKWRD 306

Query: 324 AGASLFGGCCRTTPNTIQGIAKSLSSK 351
            GA L GGCCRTTP+TI  I++ L  +
Sbjct: 307 LGAKLIGGCCRTTPSTINAISRDLKRR 326

BLAST of Carg10035 vs. Swiss-Prot
Match: sp|Q8LAX0|HMT3_ARATH (Homocysteine S-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=HMT3 PE=1 SV=2)

HSP 1 Score: 516.5 bits (1329), Expect = 2.3e-145
Identity = 243/332 (73.19%), Postives = 286/332 (86.14%), Query Frame = 0

Query: 19  VPAETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHLD 78
           V  ET++ MTDFL +CGGY V+DGG ATEL+RHGAD+NDPLWSAKCL++SPH+V +VHLD
Sbjct: 5   VKEETSSLMTDFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTKVHLD 64

Query: 79  YLDAGANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTKDSWDW 138
           YL++GANII TASYQATIQGF AKG S  E+E LLR+SVEI  EAREI+  RCTK SWD+
Sbjct: 65  YLESGANIIITASYQATIQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDF 124

Query: 139 TASERGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGAD 198
             + + S RPILVAASVGSYGAYLADGSEYSG YGDSVS ETLKDFHRRRVQILA +GAD
Sbjct: 125 AYAGKASRRPILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGAD 184

Query: 199 LIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIADASKRV 258
           LIAFETIPNKLEA+AYA+LL+EE I+IPAWFSF SKDG++V  GDS+ +CA +AD+ K V
Sbjct: 185 LIAFETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVADSCKNV 244

Query: 259 VAVGINCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGEDFVSYV 318
           VA+GINCT PRYIH LI S+R++T KPI++YPNSGE YDG  K+W+KS+G + EDFVSYV
Sbjct: 245 VAIGINCTAPRYIHALIISLRQMTRKPIVVYPNSGEVYDGLNKKWIKSEGESEEDFVSYV 304

Query: 319 SKWKEAGASLFGGCCRTTPNTIQGIAKSLSSK 351
           SKW++AGASLFGGCCRTTPNTI+ IAK LS +
Sbjct: 305 SKWRDAGASLFGGCCRTTPNTIRAIAKVLSDE 336

BLAST of Carg10035 vs. Swiss-Prot
Match: sp|Q9M1W4|HMT2_ARATH (Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HMT-2 PE=1 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 2.6e-133
Identity = 229/325 (70.46%), Postives = 275/325 (84.62%), Query Frame = 0

Query: 27  MTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHLDYLDAGANI 86
           M DFL+Q GGY VIDGGLATE ERHGADLNDPLWSAKCLV+SPH++  VHLDYL+AGA+I
Sbjct: 9   MKDFLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAGADI 68

Query: 87  ITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERC-TKDSWDWTASERGS 146
           I++ASYQATIQGFEAKGFSR+ESE LL+KSVEIA EAR  Y ++C T  S D    ++  
Sbjct: 69  ISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMDDKILKK-- 128

Query: 147 TRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGADLIAFETI 206
            RPILVAASVGSYGAYLADGSEYSG YGDS++LE LKDFHRRR+Q+LA +GADLIAFETI
Sbjct: 129 -RPILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETI 188

Query: 207 PNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIADASKRVVAVGINC 266
           PNK+EAQA+A+LL+E  ++IP WFSFNSKDG+NVVSGDSI +C SIA+  ++VVAVGINC
Sbjct: 189 PNKIEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINC 248

Query: 267 TPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGEDFVSYVSKWKEAG 326
           TPPR+I GL+  I +VT+KPIL+YPNSGE+YD + K+W+++ G+  EDFVSYV KW +AG
Sbjct: 249 TPPRFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENTGVGDEDFVSYVEKWMDAG 308

Query: 327 ASLFGGCCRTTPNTIQGIAKSLSSK 351
            SL GGCCRTTP TI+ I K L ++
Sbjct: 309 VSLLGGCCRTTPTTIRAIHKRLVNR 330

BLAST of Carg10035 vs. Swiss-Prot
Match: sp|Q4VNK0|SMTA_BRAOT (Selenocysteine Se-methyltransferase OS=Brassica oleracea var. italica OX=36774 GN=SMT PE=1 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 3.7e-132
Identity = 230/330 (69.70%), Postives = 276/330 (83.64%), Query Frame = 0

Query: 21  AETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHLDYL 80
           AET   M + L++ GGY +IDGGLATELERHGADLNDPLWSAKCL++SPH++  VHLDYL
Sbjct: 8   AETFYSMKELLKETGGYAIIDGGLATELERHGADLNDPLWSAKCLLTSPHLIHTVHLDYL 67

Query: 81  DAGANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTKDSWDWTA 140
           +AGA+II++ASYQATIQGFEAKG+S ++SE LLRKSVEIA EAR  Y ++C KD  D   
Sbjct: 68  EAGADIISSASYQATIQGFEAKGYSIEKSESLLRKSVEIACEARSTYYDKC-KDDDDKKI 127

Query: 141 SERGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGADLI 200
            ++   RPILVAASVGSYGA+LADGSEYSG YGD ++LETLKDFHRRRVQ+LA +GAD+I
Sbjct: 128 LKK---RPILVAASVGSYGAFLADGSEYSGIYGDLITLETLKDFHRRRVQVLAESGADII 187

Query: 201 AFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIADASKRVVA 260
           AFETIPNKLEAQA+AELLDE   +IP WFSFNSKDG+NVVSGDSI +C +IA+A ++VVA
Sbjct: 188 AFETIPNKLEAQAFAELLDEGVAKIPGWFSFNSKDGVNVVSGDSIKECIAIAEACEKVVA 247

Query: 261 VGINCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGEDFVSYVSK 320
           VGINCTPPR+I GL+  I +VT+KPIL+YPNSGE YD E K+W+++ G+  EDFVSYV K
Sbjct: 248 VGINCTPPRFIEGLVLEIAKVTSKPILVYPNSGERYDPERKEWVENTGVGNEDFVSYVEK 307

Query: 321 WKEAGASLFGGCCRTTPNTIQGIAKSLSSK 351
           W +AG SL GGCCRTTP TI+ I K L S+
Sbjct: 308 WMDAGVSLLGGCCRTTPTTIRAIHKRLVSR 333

BLAST of Carg10035 vs. Swiss-Prot
Match: sp|P56707|SMTA_ASTBI (Selenocysteine methyltransferase OS=Astragalus bisulcatus OX=20406 GN=SMTA PE=1 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 2.5e-128
Identity = 224/328 (68.29%), Postives = 269/328 (82.01%), Query Frame = 0

Query: 23  TTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHLDYLDA 82
           ++  +TDFL Q G   VI GGL TEL+RHGADLNDPLWSAKCL+S PH++R+VHLDYL+ 
Sbjct: 2   SSPLITDFLHQAGRAAVIAGGLGTELQRHGADLNDPLWSAKCLLSCPHLIRQVHLDYLEN 61

Query: 83  GANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTKDSWDWTASE 142
           GA+II TASYQATIQGF+AKGFS +E E LLR+SVEIA EAR++Y +RC + S D     
Sbjct: 62  GADIIITASYQATIQGFKAKGFSDEEGEALLRRSVEIAREARDLYYQRCAESSSDNGDDS 121

Query: 143 R-GSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGADLIA 202
           R    RPIL+A SVGSYGAYLADGSE+SG YGD++  ETLKDFHRR+VQILA++G DL+A
Sbjct: 122 RILKQRPILIAGSVGSYGAYLADGSEFSGNYGDAIKSETLKDFHRRKVQILADSGVDLLA 181

Query: 203 FETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIADASKRVVAV 262
           FE +PNKLEAQAYA+LL+EE I  PAWF+F SKDG NVVSGDSI +C SIA++  +VVAV
Sbjct: 182 FEAVPNKLEAQAYADLLEEENIITPAWFAFTSKDGNNVVSGDSIEECGSIAESCDKVVAV 241

Query: 263 GINCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGEDFVSYVSKW 322
           GINCTPPR+IH LI  +++VT KPI+IYPNSGETYD   K+W ++ G+T EDFVSYV KW
Sbjct: 242 GINCTPPRFIHDLILLLKKVTAKPIVIYPNSGETYDAIRKEWGQNSGVTDEDFVSYVDKW 301

Query: 323 KEAGASLFGGCCRTTPNTIQGIAKSLSS 350
            E+GASL GGCCRTTP+TI+GI K LSS
Sbjct: 302 CESGASLVGGCCRTTPDTIRGIYKILSS 329

BLAST of Carg10035 vs. Swiss-Prot
Match: sp|Q9FUM9|HMT2_MAIZE (Homocysteine S-methyltransferase 2 OS=Zea mays OX=4577 GN=HMT-2 PE=2 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 2.2e-124
Identity = 217/314 (69.11%), Postives = 264/314 (84.08%), Query Frame = 0

Query: 35  GGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHLDYLDAGANIITTASYQA 94
           GG  V+DGGLATELE +GADLNDPLWSAKCL+SSPH++R+VH+DYL+AGANII TASYQA
Sbjct: 21  GGRLVLDGGLATELEANGADLNDPLWSAKCLLSSPHLIRKVHMDYLEAGANIIITASYQA 80

Query: 95  TIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTKDSWDWTASERGSTRPILVAAS 154
           TIQGFE+KGFS+++SE LL KSVEIALEARE++L+   + S            P+LVAAS
Sbjct: 81  TIQGFESKGFSKEQSENLLTKSVEIALEAREMFLKEHLEKS-------TPIQHPVLVAAS 140

Query: 155 VGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGADLIAFETIPNKLEAQAY 214
           +GSYGAYLADGSEYSG YG++ + E LKDFHRRR+Q+LA AG DLIAFETIPNKLEA+AY
Sbjct: 141 LGSYGAYLADGSEYSGDYGEAGTKEFLKDFHRRRLQVLAEAGPDLIAFETIPNKLEAEAY 200

Query: 215 AELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIADASKRVVAVGINCTPPRYIHGL 274
            ELL+E  I IPAWFSFNSKDG+++VSGDS+ +C +IAD   +V AVGINCTPPR+IHGL
Sbjct: 201 VELLEECNINIPAWFSFNSKDGVHIVSGDSLIECTTIADKCAKVGAVGINCTPPRFIHGL 260

Query: 275 ISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGEDFVSYVSKWKEAGASLFGGCCR 334
           I SIR+VT+KPILIYPNSGE YDGE K+W++S G++  DFVSYV++W + GA L GGCCR
Sbjct: 261 ILSIRKVTDKPILIYPNSGERYDGEKKEWVESTGVSDGDFVSYVNEWCKDGAVLIGGCCR 320

Query: 335 TTPNTIQGIAKSLS 349
           TTPNTI+ I ++L+
Sbjct: 321 TTPNTIRAIHRTLN 327

BLAST of Carg10035 vs. TrEMBL
Match: tr|A0A1S3BMQ2|A0A1S3BMQ2_CUCME (homocysteine S-methyltransferase 3 OS=Cucumis melo OX=3656 GN=LOC103491241 PE=4 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 8.3e-169
Identity = 287/335 (85.67%), Postives = 320/335 (95.52%), Query Frame = 0

Query: 18  RVPAETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHL 77
           ++P   TT++T FL++CGGYGV+DGG ATELER GADLNDPLWSAKCL++SPH+VRRVHL
Sbjct: 4   QMPETATTYLTHFLQKCGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHL 63

Query: 78  DYLDAGANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTKDSWD 137
           DYLDAGANII+TASYQAT+QGFEAKGFSRDESE+LLRKSV+IA+EAR+IYLERCTKDSWD
Sbjct: 64  DYLDAGANIISTASYQATLQGFEAKGFSRDESEDLLRKSVQIAIEARDIYLERCTKDSWD 123

Query: 138 WTASERGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGA 197
           +T +  GS RP+L+AAS+GSYGAYLADGSEYSG+YG+SV+LETLK+FHRRRVQILANAGA
Sbjct: 124 FTETGAGSRRPVLIAASIGSYGAYLADGSEYSGEYGESVTLETLKNFHRRRVQILANAGA 183

Query: 198 DLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIADASKR 257
           DLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSI+DCASIADASK+
Sbjct: 184 DLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQ 243

Query: 258 VVAVGINCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGEDFVSY 317
           VVAVGINCTPPRYIHGLI SI+EVT+KPI++YPNSGETYDG TKQWLKSDGM GEDFVSY
Sbjct: 244 VVAVGINCTPPRYIHGLILSIQEVTDKPIVVYPNSGETYDGVTKQWLKSDGMIGEDFVSY 303

Query: 318 VSKWKEAGASLFGGCCRTTPNTIQGIAKSLSSKAF 353
           V KWKEAGASLFGGCCRTTPNTI+GIAK+LSSK F
Sbjct: 304 VRKWKEAGASLFGGCCRTTPNTIRGIAKALSSKTF 338

BLAST of Carg10035 vs. TrEMBL
Match: tr|A0A0A0LKT7|A0A0A0LKT7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G223160 PE=4 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 1.4e-168
Identity = 290/335 (86.57%), Postives = 317/335 (94.63%), Query Frame = 0

Query: 18  RVPAETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHL 77
           ++P   TTF+T FL+  GGYGV+DGG ATELER GADLNDPLWSAKCL++SPH+VRRVHL
Sbjct: 4   QMPETATTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHL 63

Query: 78  DYLDAGANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTKDSWD 137
           DYLDAGANII+TASYQATIQGFEAKGFSRDESE LLRKSV+IA+EAR+IYLERCTKDSWD
Sbjct: 64  DYLDAGANIISTASYQATIQGFEAKGFSRDESENLLRKSVQIAIEARDIYLERCTKDSWD 123

Query: 138 WTASERGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGA 197
           +T +  GS RPIL+AAS+GSYGAYLADGSEYSG+YGDSV+LETLKDFHRRRVQILANAGA
Sbjct: 124 FTETGAGSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGA 183

Query: 198 DLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIADASKR 257
           DLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSI+DCASIADASK+
Sbjct: 184 DLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQ 243

Query: 258 VVAVGINCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGEDFVSY 317
           VVAVGINCTPPRYIHGLI SIREVT+KPI++YPNSGE+YDG TK+WLKSDGM GEDFVSY
Sbjct: 244 VVAVGINCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSY 303

Query: 318 VSKWKEAGASLFGGCCRTTPNTIQGIAKSLSSKAF 353
           V KWKEAGASLFGGCCRTTPNTI+GIAK+LSSK F
Sbjct: 304 VRKWKEAGASLFGGCCRTTPNTIRGIAKALSSKTF 338

BLAST of Carg10035 vs. TrEMBL
Match: tr|A0A2P4MGQ0|A0A2P4MGQ0_QUESU (Homocysteine s-methyltransferase 3 OS=Quercus suber OX=58331 GN=CFP56_73476 PE=4 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 1.8e-155
Identity = 266/325 (81.85%), Postives = 300/325 (92.31%), Query Frame = 0

Query: 26  FMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHLDYLDAGAN 85
           FM+DF+ +CGGY VIDGG ATELERHGADLNDPLWSAKCLV SPH+VR+VHLDYLDAGAN
Sbjct: 19  FMSDFIGKCGGYVVIDGGFATELERHGADLNDPLWSAKCLVDSPHLVRKVHLDYLDAGAN 78

Query: 86  IITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTKDSWDWTASERGS 145
           II TASYQATIQGFEAKGFS++E+E LLRKSVEIA EAREIY +RCTKDSWD+  + R S
Sbjct: 79  IILTASYQATIQGFEAKGFSKEEAEALLRKSVEIACEAREIYYDRCTKDSWDFMENGRTS 138

Query: 146 TRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGADLIAFETI 205
            RPILVAASVGSYGAYLADGSEYSG YGD+++LETLKDF+RRRVQILAN+GADLIAFETI
Sbjct: 139 RRPILVAASVGSYGAYLADGSEYSGNYGDAITLETLKDFNRRRVQILANSGADLIAFETI 198

Query: 206 PNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIADASKRVVAVGINC 265
           PNKLEA+AYAELL+EEG+EIPAWFSFNSKDGINVVSGD I++C SIAD+ K+VVAVGINC
Sbjct: 199 PNKLEAKAYAELLEEEGMEIPAWFSFNSKDGINVVSGDPISECVSIADSCKQVVAVGINC 258

Query: 266 TPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGEDFVSYVSKWKEAG 325
           TPPR+IHGLI+SIR+VT+KP+LIYPNSGETYDG+TKQW+KS G   EDFVSY+ KW+EAG
Sbjct: 259 TPPRFIHGLITSIRKVTSKPVLIYPNSGETYDGQTKQWVKSSGNVDEDFVSYIVKWREAG 318

Query: 326 ASLFGGCCRTTPNTIQGIAKSLSSK 351
           ASLFGGCCRTTP TI+GIA +LS K
Sbjct: 319 ASLFGGCCRTTPKTIRGIATALSDK 343

BLAST of Carg10035 vs. TrEMBL
Match: tr|V4S391|V4S391_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10005328mg PE=4 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 1.5e-154
Identity = 263/329 (79.94%), Postives = 304/329 (92.40%), Query Frame = 0

Query: 23  TTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHLDYLDA 82
           TT+FMTDFL++CGGY V+DGG ATELERHGADLNDPLWSAKCLVSSPH+VR+VHLDYLDA
Sbjct: 8   TTSFMTDFLQKCGGYSVVDGGFATELERHGADLNDPLWSAKCLVSSPHLVRKVHLDYLDA 67

Query: 83  GANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTKDSWDWTASE 142
           GANII TASYQATIQGFEAKGFS +E+E LLR+SVEIA EAREIY +RC KDSWD+T S 
Sbjct: 68  GANIIITASYQATIQGFEAKGFSTEEAEALLRRSVEIACEAREIYYDRCMKDSWDFTGSG 127

Query: 143 RGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGADLIAF 202
           R S+RP+LVAASVGSYGAYLADGSEYSG YGD+VSLETLK+FHRRRVQILAN+GADLIAF
Sbjct: 128 RISSRPVLVAASVGSYGAYLADGSEYSGDYGDAVSLETLKEFHRRRVQILANSGADLIAF 187

Query: 203 ETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIADASKRVVAVG 262
           ETIPNKLEA+AYAELL+EEGI IPAWFSFNSKDGINVVSGDSI +CASIAD+ ++VVAVG
Sbjct: 188 ETIPNKLEAKAYAELLEEEGITIPAWFSFNSKDGINVVSGDSILECASIADSCEQVVAVG 247

Query: 263 INCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGEDFVSYVSKWK 322
           INCT PR+IHGLI S+R+VT+KP++IYPNSGETY+ E K+W++S G+  EDFVSY+ KW+
Sbjct: 248 INCTSPRFIHGLILSVRKVTSKPVIIYPNSGETYNAELKKWVESTGVRDEDFVSYIGKWR 307

Query: 323 EAGASLFGGCCRTTPNTIQGIAKSLSSKA 352
           +AGASLFGGCCRTTPNTI+ I++ LS+K+
Sbjct: 308 DAGASLFGGCCRTTPNTIKAISRVLSNKS 336

BLAST of Carg10035 vs. TrEMBL
Match: tr|A0A2H5Q232|A0A2H5Q232_CITUN (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_186410 PE=4 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 1.5e-154
Identity = 263/329 (79.94%), Postives = 304/329 (92.40%), Query Frame = 0

Query: 23  TTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHLDYLDA 82
           TT+FMTDFL++CGGY V+DGG ATELERHGADLNDPLWSAKCLVSSPH+VR+VHLDYLDA
Sbjct: 8   TTSFMTDFLQKCGGYSVVDGGFATELERHGADLNDPLWSAKCLVSSPHLVRKVHLDYLDA 67

Query: 83  GANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTKDSWDWTASE 142
           GANII TASYQATIQGFEAKGFS +E+E LLR+SVEIA EAREIY +RC KDSWD+T S 
Sbjct: 68  GANIIITASYQATIQGFEAKGFSTEEAEALLRRSVEIACEAREIYYDRCMKDSWDFTGSG 127

Query: 143 RGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGADLIAF 202
           R S+RP+LVAASVGSYGAYLADGSEYSG YGD+VSLETLK+FHRRRVQILAN+GADLIAF
Sbjct: 128 RISSRPVLVAASVGSYGAYLADGSEYSGDYGDAVSLETLKEFHRRRVQILANSGADLIAF 187

Query: 203 ETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIADASKRVVAVG 262
           ETIPNKLEA+AYAELL+EEGI IPAWFSFNSKDGINVVSGDSI +CASIAD+ ++VVAVG
Sbjct: 188 ETIPNKLEAKAYAELLEEEGITIPAWFSFNSKDGINVVSGDSILECASIADSCEQVVAVG 247

Query: 263 INCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGEDFVSYVSKWK 322
           INCT PR+IHGLI S+R+VT+KP++IYPNSGETY+ E K+W++S G+  EDFVSY+ KW+
Sbjct: 248 INCTSPRFIHGLILSVRKVTSKPVIIYPNSGETYNAELKKWVESTGVRDEDFVSYIGKWR 307

Query: 323 EAGASLFGGCCRTTPNTIQGIAKSLSSKA 352
           +AGASLFGGCCRTTPNTI+ I++ LS+K+
Sbjct: 308 DAGASLFGGCCRTTPNTIKAISRVLSNKS 336

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022959458.15.2e-19998.86homocysteine S-methyltransferase 3-like [Cucurbita moschata] >XP_022959459.1 hom... [more]
XP_023549248.15.6e-19399.12homocysteine S-methyltransferase 3-like [Cucurbita pepo subsp. pepo][more]
XP_023006735.13.2e-18897.05homocysteine S-methyltransferase 3-like [Cucurbita maxima] >XP_023006736.1 homoc... [more]
XP_008449335.11.3e-16885.67PREDICTED: homocysteine S-methyltransferase 3 [Cucumis melo][more]
XP_004145385.12.1e-16886.57PREDICTED: homocysteine S-methyltransferase 3 [Cucumis sativus] >KGN61674.1 hypo... [more]
Match NameE-valueIdentityDescription
AT3G22740.11.3e-14673.19homocysteine S-methyltransferase 3[more]
AT3G63250.11.4e-13470.46homocysteine methyltransferase 2[more]
AT3G25900.15.9e-10453.82Homocysteine S-methyltransferase family protein[more]
Match NameE-valueIdentityDescription
sp|Q8LAX0|HMT3_ARATH2.3e-14573.19Homocysteine S-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=HMT3 PE=1 ... [more]
sp|Q9M1W4|HMT2_ARATH2.6e-13370.46Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HMT-2 PE=1... [more]
sp|Q4VNK0|SMTA_BRAOT3.7e-13269.70Selenocysteine Se-methyltransferase OS=Brassica oleracea var. italica OX=36774 G... [more]
sp|P56707|SMTA_ASTBI2.5e-12868.29Selenocysteine methyltransferase OS=Astragalus bisulcatus OX=20406 GN=SMTA PE=1 ... [more]
sp|Q9FUM9|HMT2_MAIZE2.2e-12469.11Homocysteine S-methyltransferase 2 OS=Zea mays OX=4577 GN=HMT-2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A1S3BMQ2|A0A1S3BMQ2_CUCME8.3e-16985.67homocysteine S-methyltransferase 3 OS=Cucumis melo OX=3656 GN=LOC103491241 PE=4 ... [more]
tr|A0A0A0LKT7|A0A0A0LKT7_CUCSA1.4e-16886.57Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G223160 PE=4 SV=1[more]
tr|A0A2P4MGQ0|A0A2P4MGQ0_QUESU1.8e-15581.85Homocysteine s-methyltransferase 3 OS=Quercus suber OX=58331 GN=CFP56_73476 PE=4... [more]
tr|V4S391|V4S391_9ROSI1.5e-15479.94Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10005328mg PE=4 ... [more]
tr|A0A2H5Q232|A0A2H5Q232_CITUN1.5e-15479.94Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_186410 PE=4 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0047150betaine-homocysteine S-methyltransferase activity
GO:0008270zinc ion binding
Vocabulary: Biological Process
TermDefinition
GO:0009086methionine biosynthetic process
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
Vocabulary: INTERPRO
TermDefinition
IPR017226Betaine-hCys_S-MeTrfase_BHMT
IPR003726HCY_dom
IPR036589HCY_dom_sf
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006544 glycine metabolic process
biological_process GO:0006563 L-serine metabolic process
biological_process GO:0009086 methionine biosynthetic process
biological_process GO:0006566 threonine metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0047150 betaine-homocysteine S-methyltransferase activity
molecular_function GO:0008270 zinc ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10035-RACarg10035-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036589Homocysteine-binding domain superfamilyGENE3DG3DSA:3.20.20.330coord: 29..346
e-value: 1.5E-106
score: 358.1
IPR036589Homocysteine-binding domain superfamilySUPERFAMILYSSF82282Homocysteine S-methyltransferasecoord: 35..349
IPR003726Homocysteine-binding domainPFAMPF02574S-methyl_transcoord: 39..346
e-value: 1.4E-78
score: 264.3
IPR003726Homocysteine-binding domainPROSITEPS50970HCYcoord: 26..347
score: 47.986
IPR017226Betaine-homocysteine S-methyltransferase, BHMTPIRSFPIRSF037505BHMTcoord: 31..225
e-value: 6.5E-7
score: 26.8
coord: 298..351
e-value: 3.9
score: 4.5
NoneNo IPR availablePANTHERPTHR21091:SF159HOMOCYSTEINE S-METHYLTRANSFERASE 3coord: 22..350
NoneNo IPR availablePANTHERPTHR21091METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATEDcoord: 22..350

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg10035Carg15892Silver-seed gourdcarcarB260