Carg08101 (gene) Silver-seed gourd

NameCarg08101
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionAspartate--tRNA(Asp/Asn) ligase
LocationCucurbita_argyrosperma_scaffold_050 : 725131 .. 730060 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCACTCGGCCGTCTTCATCCACACGCAGAGTCTTCTTGTTCACCTTTCCTCTGCGAGCGCTCCGTTTCCAGAATCCTTAAACCCTTCCGCCTAACTTCGCCGCTTCCATCAATCTTCCATTGATCCACCTTCAAACCTTCCGCCATGTCCCGCTCTATTCTCCGAGCTCTCCCTTTCTCCACCCTTCGCCACAAGCCTCCTCAATTTCTCCCCATCTTCCACCGTATTTCTCATCTCCGCCTCGCGAAATCCATTTCCAGAACTCTGAGACCTATTTCCTCACTCTCTGTTTCCGCCACTCATCCGCCTTCCTCCGCCACCGAAACGCTCGTTTCCAGTTCGAAGTCTCCGTCGCCTCTTAAGGATTCTCTTCAGTGGATCAATCGTACTGCGTTCTGTGGTGAATTGTCGGCTGGCGATGTTGGGAAGCGGGTTCGGCTTTGTGGTTGGGTTGCACTACATAGGGTTCATGGTGGTTTGACTTTTCTCAATCTTAGAGACCACAGTGGTATCGTACAGGTTTGTTACTTCTGTTTGAATTTTTGCGATTAGCTTGAAGTTGAAGAAGCTTTATTGTTGAGTGTTGATTTTTACTGCTTGAATTAGGTGACTACGCTACCTAACGAGTTTCCTGATGCTCATTCGGTTATTAACGACTTGCGGCTCGAGTATGTTGTTGCTATAGAAGGCATTGTACGGTCTCGACCTGATGATTCGATTAATGTGAAAATGAAAACTGGCTTGATAGAGGTATTTATGAATTTCGATTTCATTTTACTCCTCATTTTGCCAGGCATTTGGTTAGAAAGATTTTTAGATTTCTCGCTTATTGCATTATGCTCATGCCTGCTCCCCACGGCCCCTTTAGCCAGCTCCTGAATGAACCAGAATTTTAAGTTATTAAGTCCTCAATCTCGGACTTGAACCTAGTATCTGGGAGGAATTTCCAAACATCCCAAGTCTTTGCAAAATAAATATGTTAATTCTTAGTGGCAATAATCCTGGGAGTTCTTGATCATGGTTGTTAAAACTCGAGATAGGCTAGTCGTGGTATAATTTTGTAGTTCTTTTGGGATATCTTGGTGCTTCCAAATAAATTCTGATTCTACAGTGGTACACCTGCGTGGTGTTTAGCTCAACTCAAAGGCAAGAATTCTAAGCTTGTAGCAATTAATCCAATTTTTAAAGCTTTAACGGAGTGAAACTCCATGGTTTTTTGAAGAAGGGGCGAGAGATTGGGTTCGAGCCTGTGATTTAGTTGACTTTATGGGCTCTTCATGGATTATTCTCACTAGTTCATTTTGTATTATAATGGTTTCGTGATCAATTTGAATTGAAGTGACTTTTTATAAGTTCCCATGGTTGCCTTTCTTGTAGCTTCTCAATTATTGGATGAAAAGTAACGTGTCTTACTAAGTGAAGGGATATGGGTTGTAGGGTAGTTGATGCCGATTGAAGTAGTATGGAGTTCGAAACTTTTTAACTTTGTTATCTTAGTTCTCTTCTCTAGGACTATGTAAGTTTCCTATGACGCAACTATCATTCTATTTTTTCAGTTTTTGTTCTGATAATTTCTTGATATATTATAGGTTGCTGCAGAGCATGTTCAAATCTTAAATTCTGTTGGATCAAAGTTACCTTTTCTAGTTACAATTGCTGATGATGCAAAGGATTCTGCTAAAGAGGAAATTCGATTGAGGTTGTTATTTCATTCTATCATTTCAGGCCAAGTTCTAATTTATATTTGTTCACTCAAACCAGATGCACAGTTTGATCCCATATTCTACGTGCTTAATTGAATCAAATGTCTGAAATCATTTGATCATCATTCTTCTTTTTTGGGTTGGGGGGAGTGAGTTAGGCGCACAGAAAAATTTCAACTTCCTTCCCTTCTTAGATCTTTCTTTAGACTTTCATTGAAGGGTGAATGCCGTGAAATAAGTTCTCTCCCTTATCTAAGATGCCCATGGGTTTGAGATGATCACATTTTTTATTTTCTCGGAACCAAATGCTATCTGGTTTTAGATTATAAGGAGTTGAGTATTGATAGGTACCGATATCTTGATCTTCGGAGGAAGCAAATGAATTCGAACATAATGCTTCGGCATAAAGTGGTGAAACTCATTCGAAGATATTTGGAGGATGCACATGACTTTGTGGAGGTACTGCCCTGTTTATAACTTTTAACTGACCGTTGACCCTTTTTTCTGAAAATTTCTTGAATCATTCTCTGCATTAGAACTTCACATTGTACTAAATAAATGGTTAGAAATTGAATTTGTTCTAGCCTTATTTCCACTTTGAACTTTTGTTGGTTATAAGGAAGATTGAGACGCCAATACTTTCTAGATCTACTCCAGAAGGTGCTCGAGATTATTTGGTGCCGTCTAGAGTTCAGGTAGCCTACAAATCCTCAACTTCTGTTGATTTAATTCTCTCAAGGCAGGTTATTTTGGTAACTAACTGGTATTTTTTGATCCAGAAACTTAAAATAAATTTCTTAAGATATCTGAATGTGATGCCATTTTCAATTTCTAGATCTATTCTCAGTTGCCCTTTTTGAATGGTATACCTTGTTAATTCATATCCATAAGTAAGTGCTAAGCATCTCATAAGACAATTTTTTTTTTTTAATTCCATTTTAAGATATCTTGCGCTAATGGTTTATAAACTGAAATGGAAGTGCTAAGATATAGTTCAGTTCTAATAGTGTATGCCCAGGTGCACATTTTCTGGTCTCACAGAATGACATCATCACCATCTTCTTCTTCTTAATGGTTTAACACATAGAATAGTAACGTGAATTCTCTTCAATTGAATGCCTTTGTTTGAAGTTTTCATGTGAAGTATTTGTCTTTTCCTATAAAGAATGTAGCATAATTGTGATTTCCTACCAAAAATATAACATAATTGTGTTTATTTAAAGTTTTTTTTTTTTGCTATATGGATTCTGTTTTTGCCTGGCTTGCAGGTCTGATTCAGTTTAAATGTGTATTTTGAAGCCAGGAACATTTTATGCTTTGCCTCAAAGTCCACAGCTGTTTAAGCAAATGTTGATGGTGTCTGGTTTTGATAAATATTATCAAGTAGCAAGGTACTGACTATTATATCATTAAGCACTACTTCCATTATGTTTTGTCTAGTTTTGACCATCTGACTTGCATCTACTATTATATTATCTCAATCTTCCCACATTTCCCAGTAAATTCGTTTTATGTTTATGCATTCTCTTTCTTCACAACCGAGATGGTATGTATAGAAAGTTCGTGCATAGATCTAGGTTGATAGATAGTTTCGTTGTTCTTATAAAAATTGGGAGCTAAGTTCAATATGTTTTCAACCCTTTTGAGTTGTGAGAATCGGGTGGTTGTTCTAAGTATGTAACATAAATTGTATTTGCACCGTCAGTTTCATTGTGGATTTCTTTGTAGGTGTTTCCGAGATGAAGACCTAAGAGCGGACAGACAACCTGAGTTTACACAACTTGATATGGAGTTAGCATTTACAACTTTTGAAGACATGCTGAAACTCAATGAAGATTTGATTAGAAGGGTAAACTCTGACTATTAGAATTGACTTGAAGATTGAGTTTCAGAATACATAAGCATAGGTTGTAGTGTAAAAATATTTGAAAAGAAACAAAGATAATTGTCACGATCTTCTGTTCTCACCACCAATTTTTTGCCACGCTTTTTTTGTTGGCACCTATTTTATGCTTGTGTGCATCGTTACCTTTTTGTAATTCGGGTAAAATAAACGTTGAAGGTCATTAAATTTATGTGATTTATGTTGGAGGGATCCAATTTTGTTACCACCTTGTCAACTTTGTTGACACTCATCAGGTTCTTTTCATTAACTTATGTCAATATGAGGAAGTAACTCCATTCAAGAGGGGGATGGACCAATGGATGTTCTAAAGGTCCAAGGCCTAAAAGTACCACTTTTAGTTATATGTTATTATTTGCCCTGCTATTTAATGGCTACTGATGGTAAAGAAAAATTATACTCATAGGTTTTTTGGGAAGTCAAAGGTGTTCAGCTGCCAAATCCTTTCCCGAGGCTTACCTATGCTGAAGCAATGAGTCGATATGGGTCAGATAAGCCTGATACAAGTTTTGATCTTCAATTGAAAGATGTAACTCCTCTTTATACTTCTGGGCCTCATTCTGACTCATGTTTTCGAATGAACTTGTTTTTTATTATGTATTTCCTTAACTAATGAGTGTGCATTGGAAGAAGGAGCCTTCTGGCTTTTGTTGTTCCTTAAAGCAGTTGTTTATTGATTTAAATTATCGCATTACTTTAGAGAATATTCAGTACTTTTTTCATTGTTCGTGCATGTCTGGATGGCCTCAGGATTCATGCTTAACATCCTTATTCAATCATTTTAATTGAATTGGCCTTTTCATAATATGGCTTTCCGGATACCAGTTCTAGGCATTTTAGCAGATTTGATGGGAAAGATTTTCTGGAATATGGTTGGTTGTTGATAAAAAAAAGGATTCTGGAATATGGTCAACTACTTATATGTTAAAGGATTTTGAAACTTATGGATTTATCATCTCAAATTATAGTCAACCATTTTTAGTTTTCAAAGCCCCATTTCCAATTCTACATTCGGCAATAGTATTGTTCACAGGAATTGTGTAATTTGTATGAATTAAAGATTCCTCTAAATTAGCTACTGAGAGATTTTTCTCATTGGAACTGCAGGTATCTGATATTTTTGCAGATTCTCCATTCAAGGTTTTTGCAGACGCCTTGTCTAGTGGTGGAATCATCAAAGTGCTGTGTGTGCCCTCTGGTGCTAAAAGGTATTCAAACACATCTCTGAAAAAGGGTGACATTTATTCAGAGGCAATCAAATCTGGAGCTAAAGGATTACCTTTTCTGAAGGTTCTTGATGATGGTAATCTTCTTTTTCTCTAA

mRNA sequence

TCCACTCGGCCGTCTTCATCCACACGCAGAGTCTTCTTGTTCACCTTTCCTCTGCGAGCGCTCCGTTTCCAGAATCCTTAAACCCTTCCGCCTAACTTCGCCGCTTCCATCAATCTTCCATTGATCCACCTTCAAACCTTCCGCCATGTCCCGCTCTATTCTCCGAGCTCTCCCTTTCTCCACCCTTCGCCACAAGCCTCCTCAATTTCTCCCCATCTTCCACCGTATTTCTCATCTCCGCCTCGCGAAATCCATTTCCAGAACTCTGAGACCTATTTCCTCACTCTCTGTTTCCGCCACTCATCCGCCTTCCTCCGCCACCGAAACGCTCGTTTCCAGTTCGAAGTCTCCGTCGCCTCTTAAGGATTCTCTTCAGTGGATCAATCGTACTGCGTTCTGTGGTGAATTGTCGGCTGGCGATGTTGGGAAGCGGGTTCGGCTTTGTGGTTGGGTTGCACTACATAGGGTTCATGGTGGTTTGACTTTTCTCAATCTTAGAGACCACAGTGGTATCGTACAGGTGACTACGCTACCTAACGAGTTTCCTGATGCTCATTCGGTTATTAACGACTTGCGGCTCGAGTATGTTGTTGCTATAGAAGGCATTGTACGGTCTCGACCTGATGATTCGATTAATGTGAAAATGAAAACTGGCTTGATAGAGGTTGCTGCAGAGCATGTTCAAATCTTAAATTCTGTTGGATCAAAGTTACCTTTTCTAGTTACAATTGCTGATGATGCAAAGGATTCTGCTAAAGAGGAAATTCGATTGAGGTACCGATATCTTGATCTTCGGAGGAAGCAAATGAATTCGAACATAATGCTTCGGCATAAAGTGGTGAAACTCATTCGAAGATATTTGGAGGATGCACATGACTTTGTGGAGATTGAGACGCCAATACTTTCTAGATCTACTCCAGAAGGTGCTCGAGATTATTTGGTGCCGTCTAGAGTTCAGCCAGGAACATTTTATGCTTTGCCTCAAAGTCCACAGCTGTTTAAGCAAATGTTGATGGTGTCTGGTTTTGATAAATATTATCAAGTAGCAAGGTGTTTCCGAGATGAAGACCTAAGAGCGGACAGACAACCTGAGTTTACACAACTTGATATGGAGTTAGCATTTACAACTTTTGAAGACATGCTGAAACTCAATGAAGATTTGATTAGAAGGGTTTTTTGGGAAGTCAAAGGTGTTCAGCTGCCAAATCCTTTCCCGAGGCTTACCTATGCTGAAGCAATGAGTCGATATGGGTCAGATAAGCCTGATACAAGTTTTGATCTTCAATTGAAAGATGTATCTGATATTTTTGCAGATTCTCCATTCAAGGTTTTTGCAGACGCCTTGTCTAGTGGTGGAATCATCAAAGTGCTGTGTGTGCCCTCTGGTGCTAAAAGGTATTCAAACACATCTCTGAAAAAGGGTGACATTTATTCAGAGGCAATCAAATCTGGAGCTAAAGGATTACCTTTTCTGAAGGTTCTTGATGATGGTAATCTTCTTTTTCTCTAA

Coding sequence (CDS)

ATGTCCCGCTCTATTCTCCGAGCTCTCCCTTTCTCCACCCTTCGCCACAAGCCTCCTCAATTTCTCCCCATCTTCCACCGTATTTCTCATCTCCGCCTCGCGAAATCCATTTCCAGAACTCTGAGACCTATTTCCTCACTCTCTGTTTCCGCCACTCATCCGCCTTCCTCCGCCACCGAAACGCTCGTTTCCAGTTCGAAGTCTCCGTCGCCTCTTAAGGATTCTCTTCAGTGGATCAATCGTACTGCGTTCTGTGGTGAATTGTCGGCTGGCGATGTTGGGAAGCGGGTTCGGCTTTGTGGTTGGGTTGCACTACATAGGGTTCATGGTGGTTTGACTTTTCTCAATCTTAGAGACCACAGTGGTATCGTACAGGTGACTACGCTACCTAACGAGTTTCCTGATGCTCATTCGGTTATTAACGACTTGCGGCTCGAGTATGTTGTTGCTATAGAAGGCATTGTACGGTCTCGACCTGATGATTCGATTAATGTGAAAATGAAAACTGGCTTGATAGAGGTTGCTGCAGAGCATGTTCAAATCTTAAATTCTGTTGGATCAAAGTTACCTTTTCTAGTTACAATTGCTGATGATGCAAAGGATTCTGCTAAAGAGGAAATTCGATTGAGGTACCGATATCTTGATCTTCGGAGGAAGCAAATGAATTCGAACATAATGCTTCGGCATAAAGTGGTGAAACTCATTCGAAGATATTTGGAGGATGCACATGACTTTGTGGAGATTGAGACGCCAATACTTTCTAGATCTACTCCAGAAGGTGCTCGAGATTATTTGGTGCCGTCTAGAGTTCAGCCAGGAACATTTTATGCTTTGCCTCAAAGTCCACAGCTGTTTAAGCAAATGTTGATGGTGTCTGGTTTTGATAAATATTATCAAGTAGCAAGGTGTTTCCGAGATGAAGACCTAAGAGCGGACAGACAACCTGAGTTTACACAACTTGATATGGAGTTAGCATTTACAACTTTTGAAGACATGCTGAAACTCAATGAAGATTTGATTAGAAGGGTTTTTTGGGAAGTCAAAGGTGTTCAGCTGCCAAATCCTTTCCCGAGGCTTACCTATGCTGAAGCAATGAGTCGATATGGGTCAGATAAGCCTGATACAAGTTTTGATCTTCAATTGAAAGATGTATCTGATATTTTTGCAGATTCTCCATTCAAGGTTTTTGCAGACGCCTTGTCTAGTGGTGGAATCATCAAAGTGCTGTGTGTGCCCTCTGGTGCTAAAAGGTATTCAAACACATCTCTGAAAAAGGGTGACATTTATTCAGAGGCAATCAAATCTGGAGCTAAAGGATTACCTTTTCTGAAGGTTCTTGATGATGGTAATCTTCTTTTTCTCTAA

Protein sequence

MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATETLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLEDAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLTYAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTSLKKGDIYSEAIKSGAKGLPFLKVLDDGNLLFL
BLAST of Carg08101 vs. NCBI nr
Match: XP_022942888.1 (aspartate--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 [Cucurbita moschata] >XP_022942889.1 aspartate--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 876.7 bits (2264), Expect = 3.4e-251
Identity = 445/448 (99.33%), Postives = 447/448 (99.78%), Query Frame = 0

Query: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60
           MSRSILRALPFS+LRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE
Sbjct: 1   MSRSILRALPFSSLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60

Query: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
           TLVSSS SPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVSSSNSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240
           ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE
Sbjct: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240

Query: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300
           DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV
Sbjct: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDD 449
           T+LKKGDIYSEAIKSGAKGLPFLKVLDD
Sbjct: 421 TALKKGDIYSEAIKSGAKGLPFLKVLDD 448

BLAST of Carg08101 vs. NCBI nr
Match: XP_023554090.1 (aspartate--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 873.2 bits (2255), Expect = 3.8e-250
Identity = 444/448 (99.11%), Postives = 445/448 (99.33%), Query Frame = 0

Query: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60
           MSRSILRALPFSTLRHKPPQFL IFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE
Sbjct: 1   MSRSILRALPFSTLRHKPPQFLSIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60

Query: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
           TLVSSS SPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVSSSNSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240
           ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE
Sbjct: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240

Query: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300
           DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV
Sbjct: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSDKPDT FDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDKPDTRFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDD 449
           T+LKKGDIYSEAIKSGAKGLPFLKVLDD
Sbjct: 421 TALKKGDIYSEAIKSGAKGLPFLKVLDD 448

BLAST of Carg08101 vs. NCBI nr
Match: XP_022975696.1 (aspartate--tRNA ligase, chloroplastic/mitochondrial-like [Cucurbita maxima] >XP_022975707.1 aspartate--tRNA ligase, chloroplastic/mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 867.5 bits (2240), Expect = 2.1e-248
Identity = 441/448 (98.44%), Postives = 443/448 (98.88%), Query Frame = 0

Query: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60
           MSRSILRALPFS LRHKPPQFLPIF RISHLRLAKSISRTLRPISSLSVSATHPPSSATE
Sbjct: 1   MSRSILRALPFSNLRHKPPQFLPIFRRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60

Query: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
           TLVSSS SPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVSSSNSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRP DSINVKMKTGLIEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPGDSINVKMKTGLIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240
           ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE
Sbjct: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240

Query: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300
           DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV
Sbjct: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YA+AMSRYGSDKPDT FDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YADAMSRYGSDKPDTRFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDD 449
           T+LKKGDIYSEAIKSGAKGLPFLKVLDD
Sbjct: 421 TALKKGDIYSEAIKSGAKGLPFLKVLDD 448

BLAST of Carg08101 vs. NCBI nr
Match: XP_004150278.2 (PREDICTED: aspartate--tRNA ligase, mitochondrial [Cucumis sativus] >KGN52733.1 hypothetical protein Csa_4G000540 [Cucumis sativus])

HSP 1 Score: 796.2 bits (2055), Expect = 5.8e-227
Identity = 400/451 (88.69%), Postives = 417/451 (92.46%), Query Frame = 0

Query: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60
           MSRS+LRA PFST+R KPPQFLPI H ISHLR  +SIS T RP+SS S SAT  PSSATE
Sbjct: 1   MSRSLLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATE 60

Query: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
            LV +S   SP KDSLQWINRTA CGELS  DVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  ILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHS INDLRLEYVVA+EG VRSRP DS+NVKMKTGLIEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240
           ILNSVGSKLPFLVT ADDAKDSAKEEIRLRYRYLDLRRKQMN NIMLRHKVVKL+RRYLE
Sbjct: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLE 240

Query: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300
           D H FVEIETPILSRSTPEGARDYLVPSRVQPGTF+ALPQSPQLFKQMLMVSGFDKYYQ+
Sbjct: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRVQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMEL+FTT EDML+LNEDLIR+VFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSD+PDT FDLQL DVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDDGNL 452
           T+LKKGDIY+EA KSGAKGLPFLKV DDG +
Sbjct: 421 TALKKGDIYTEATKSGAKGLPFLKVTDDGGI 451

BLAST of Carg08101 vs. NCBI nr
Match: XP_023551993.1 (aspartate--tRNA ligase, chloroplastic/mitochondrial-like [Cucurbita pepo subsp. pepo] >XP_023551994.1 aspartate--tRNA ligase, chloroplastic/mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 788.9 bits (2036), Expect = 9.3e-225
Identity = 399/451 (88.47%), Postives = 416/451 (92.24%), Query Frame = 0

Query: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60
           MSRS+LRA PFSTLR KPPQFLPIFH ISHLRL KSISRT R +SSLS       +SA E
Sbjct: 1   MSRSLLRAFPFSTLRPKPPQFLPIFHHISHLRLVKSISRTRRSVSSLS-------ASAAE 60

Query: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
           TLV SS S  PLKDSLQWINRTA CGELS  DVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVPSSNSQLPLKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHS INDLRLEYVVA+EG VRSRP DS+NV MKTGLIEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVNMKTGLIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240
           ILNSVGSKLPFLVT ADDAKD AKEEIRLRYRYLDLRRKQMN NIMLRHKVVKL+RRYLE
Sbjct: 181 ILNSVGSKLPFLVTTADDAKDYAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLMRRYLE 240

Query: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300
           D H FVEIETPILSRSTPEGARDYLVPSRVQPGTF+ALPQSPQLFKQMLMVSGFDKYYQ+
Sbjct: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRVQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMELAFTT EDMLKLNEDLIR+VFWEVKGVQLPNPFP+LT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTLEDMLKLNEDLIRKVFWEVKGVQLPNPFPKLT 360

Query: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           Y+EAMSRYGSD+PDT FDLQLKDVSD+FADS FKVFADALS+G IIKVLCVPSGAKRYSN
Sbjct: 361 YSEAMSRYGSDRPDTRFDLQLKDVSDVFADSSFKVFADALSNGEIIKVLCVPSGAKRYSN 420

Query: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDDGNL 452
           T+LKKGDIYSEAIKSGAKGLPFLK+LDDG +
Sbjct: 421 TALKKGDIYSEAIKSGAKGLPFLKILDDGGI 444

BLAST of Carg08101 vs. TAIR10
Match: AT4G33760.1 (tRNA synthetase class II (D, K and N) family protein)

HSP 1 Score: 619.4 bits (1596), Expect = 1.8e-177
Identity = 313/448 (69.87%), Postives = 366/448 (81.70%), Query Frame = 0

Query: 5   ILRALPFSTLRHKPPQFLPIFH-RISHLRLAKSISRTLRPISSLSVSATHPPSSATETLV 64
           +LR LP      +P +FL      IS+      + +   P+     + +  P +A    V
Sbjct: 4   LLRTLPL-----RPTRFLSATAISISNATNFFVVPKRTNPLPGTRRTFSSSPVAAASGDV 63

Query: 65  SSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGI 124
                PSP    L+W++RT  CGELS  DVGKRV LCGWVALHRVHGGLTFLNLRDH+GI
Sbjct: 64  VVKPVPSP-PSVLRWVSRTELCGELSVNDVGKRVHLCGWVALHRVHGGLTFLNLRDHTGI 123

Query: 125 VQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILN 184
           VQV TLP+EFP+AH +IND+RLEYVV +EG VRSRP++S+N KMKTG +EV AEHV+ILN
Sbjct: 124 VQVRTLPDEFPEAHGLINDMRLEYVVLVEGTVRSRPNESVNKKMKTGFVEVVAEHVEILN 183

Query: 185 SVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLEDAH 244
            V +KLPFLVT AD+ KD  KEEIRLR+R LDLRR+QM +NI+LRH VVKLIRRYLED H
Sbjct: 184 PVRTKLPFLVTTADENKDLIKEEIRLRFRCLDLRRQQMKNNIVLRHNVVKLIRRYLEDRH 243

Query: 245 DFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARC 304
            F+EIETPILSRSTPEGARDYLVPSR+Q GTFYALPQSPQLFKQMLMVSGFDKYYQ+ARC
Sbjct: 244 GFIEIETPILSRSTPEGARDYLVPSRIQSGTFYALPQSPQLFKQMLMVSGFDKYYQIARC 303

Query: 305 FRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLTYAE 364
           FRDEDLRADRQPEFTQLDME+AF   EDMLKLNEDLIR+VF E+KG+QLP+PFPRLTYA+
Sbjct: 304 FRDEDLRADRQPEFTQLDMEMAFMPMEDMLKLNEDLIRKVFSEIKGIQLPDPFPRLTYAD 363

Query: 365 AMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTSL 424
           AM RYGSD+PDT FDL+LKDVS++F +S F+VF +AL SGGIIKVLCVP GAK+YSN++L
Sbjct: 364 AMDRYGSDRPDTRFDLELKDVSNVFTESSFRVFTEALESGGIIKVLCVPLGAKKYSNSAL 423

Query: 425 KKGDIYSEAIKSGAKGLPFLKVLDDGNL 452
           KKGDIY+EA+KSGAKGLPFLKVLD+G +
Sbjct: 424 KKGDIYNEAMKSGAKGLPFLKVLDNGEI 445

BLAST of Carg08101 vs. TAIR10
Match: AT3G13490.1 (Lysyl-tRNA synthetase, class II)

HSP 1 Score: 75.9 bits (185), Expect = 7.3e-14
Identity = 68/277 (24.55%), Postives = 121/277 (43.68%), Query Frame = 0

Query: 74  DSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEF 133
           + LQ I +    GE S  ++   V + G V   R  G L FL LRD SG +Q+       
Sbjct: 114 NQLQEIYKHLANGEESDNEI-DCVSIAGRVVARRAFGKLAFLTLRDDSGTIQLYCEKERL 173

Query: 134 PDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILNSVGSKLPFLV 193
            D        +L+  + I  I+ +        + + G + +      IL         L+
Sbjct: 174 SDDQFE----QLKQFIDIGDILGA---SGSMKRTEKGELSICVNSFSILTK------SLL 233

Query: 194 TIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRH-KVVKLIRRYLEDAHDFVEIETPI 253
            + D        + R R RY+D+      +++  R  K+V  IR+ +E +  ++E+ETP+
Sbjct: 234 PLPDKYHGLTDIDKRYRQRYVDMIANPEVADVFRRRAKIVSEIRKTVE-SFGYLEVETPV 293

Query: 254 LSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARCFRDEDLRAD 313
           L  +         V      G    L  + +L  + ++V GF+K Y++ R FR+E +   
Sbjct: 294 LQGAAGGAEARPFVTFHNSLGRDLYLRIATELHLKRMLVGGFEKVYEIGRIFRNEGISTR 353

Query: 314 RQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKG 350
             PEFT ++M  A++ +  M+ + E ++ +    V G
Sbjct: 354 HNPEFTTIEMYEAYSDYHSMMDMAELIVTQCSMAVNG 375

BLAST of Carg08101 vs. TAIR10
Match: AT3G11710.1 (lysyl-tRNA synthetase 1)

HSP 1 Score: 66.6 bits (161), Expect = 4.4e-11
Identity = 38/146 (26.03%), Postives = 77/146 (52.74%), Query Frame = 0

Query: 205 EEIRLRYRYLD-LRRKQMNSNIMLRHKVVKLIRRYLEDAHDFVEIETPILSRSTPEGARD 264
           +E R R R+LD +   ++      R K++  +RR+L D  +F+E+ETP+++      A  
Sbjct: 259 QESRYRQRHLDMILNVEVRQIFRTRAKIISYVRRFL-DNKNFLEVETPMMNMIAGGAAAR 318

Query: 265 YLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARCFRDEDLRADRQPEFTQLDME 324
             V           +  +P+L+ + L+V G ++ Y++ + FR+E +     PEFT  +  
Sbjct: 319 PFVTHHNDLDMRLYMRIAPELYLKQLIVGGLERVYEIGKQFRNEGIDLTHNPEFTTCEFY 378

Query: 325 LAFTTFEDMLKLNEDLIRRVFWEVKG 350
           +AF  + D++++ E ++  +  E+ G
Sbjct: 379 MAFADYNDLMEMTEVMLSGMVKELTG 403

BLAST of Carg08101 vs. Swiss-Prot
Match: sp|F4JJT9|SYDM_ARATH (Aspartate--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g33760 PE=2 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 3.2e-176
Identity = 313/448 (69.87%), Postives = 366/448 (81.70%), Query Frame = 0

Query: 5   ILRALPFSTLRHKPPQFLPIFH-RISHLRLAKSISRTLRPISSLSVSATHPPSSATETLV 64
           +LR LP      +P +FL      IS+      + +   P+     + +  P +A    V
Sbjct: 4   LLRTLPL-----RPTRFLSATAISISNATNFFVVPKRTNPLPGTRRTFSSSPVAAASGDV 63

Query: 65  SSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGI 124
                PSP    L+W++RT  CGELS  DVGKRV LCGWVALHRVHGGLTFLNLRDH+GI
Sbjct: 64  VVKPVPSP-PSVLRWVSRTELCGELSVNDVGKRVHLCGWVALHRVHGGLTFLNLRDHTGI 123

Query: 125 VQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILN 184
           VQV TLP+EFP+AH +IND+RLEYVV +EG VRSRP++S+N KMKTG +EV AEHV+ILN
Sbjct: 124 VQVRTLPDEFPEAHGLINDMRLEYVVLVEGTVRSRPNESVNKKMKTGFVEVVAEHVEILN 183

Query: 185 SVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLEDAH 244
            V +KLPFLVT AD+ KD  KEEIRLR+R LDLRR+QM +NI+LRH VVKLIRRYLED H
Sbjct: 184 PVRTKLPFLVTTADENKDLIKEEIRLRFRCLDLRRQQMKNNIVLRHNVVKLIRRYLEDRH 243

Query: 245 DFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARC 304
            F+EIETPILSRSTPEGARDYLVPSR+Q GTFYALPQSPQLFKQMLMVSGFDKYYQ+ARC
Sbjct: 244 GFIEIETPILSRSTPEGARDYLVPSRIQSGTFYALPQSPQLFKQMLMVSGFDKYYQIARC 303

Query: 305 FRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLTYAE 364
           FRDEDLRADRQPEFTQLDME+AF   EDMLKLNEDLIR+VF E+KG+QLP+PFPRLTYA+
Sbjct: 304 FRDEDLRADRQPEFTQLDMEMAFMPMEDMLKLNEDLIRKVFSEIKGIQLPDPFPRLTYAD 363

Query: 365 AMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTSL 424
           AM RYGSD+PDT FDL+LKDVS++F +S F+VF +AL SGGIIKVLCVP GAK+YSN++L
Sbjct: 364 AMDRYGSDRPDTRFDLELKDVSNVFTESSFRVFTEALESGGIIKVLCVPLGAKKYSNSAL 423

Query: 425 KKGDIYSEAIKSGAKGLPFLKVLDDGNL 452
           KKGDIY+EA+KSGAKGLPFLKVLD+G +
Sbjct: 424 KKGDIYNEAMKSGAKGLPFLKVLDNGEI 445

BLAST of Carg08101 vs. Swiss-Prot
Match: sp|Q8YUB6|SYDND_NOSS1 (Aspartate--tRNA(Asp/Asn) ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=aspS PE=3 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 4.7e-127
Identity = 219/372 (58.87%), Postives = 292/372 (78.49%), Query Frame = 0

Query: 81  RTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSVI 140
           RT +CGEL   D+G+ V L GWV   R HGG+ FL+LRD SGIVQV + P   PD++ + 
Sbjct: 2   RTHYCGELRQKDIGETVTLYGWVDRRRDHGGVIFLDLRDRSGIVQVVSDPQRTPDSYELA 61

Query: 141 NDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILNSVGSKLPFLVTIADDAK 200
           N LR EYVV I G V  RP++S+N ++ TG +E+ A+ +++LN V  +LPF V+ AD   
Sbjct: 62  NSLRNEYVVEITGRVTQRPEESLNTRIPTGEVEIYADKIELLNGVRKQLPFQVSTAD--T 121

Query: 201 DSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLEDAHDFVEIETPILSRSTPEG 260
           ++ +E++RL+YRYLDLRR +M  NI LRH+VVK +RRYLED   F+E+ETPIL+RSTPEG
Sbjct: 122 ETVREDLRLKYRYLDLRRDRMARNIQLRHQVVKAMRRYLEDVEGFIEVETPILTRSTPEG 181

Query: 261 ARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARCFRDEDLRADRQPEFTQL 320
           ARDY++PSRV PG ++ALPQSPQLFKQ+LMVSG D+YYQ+ARCFRDEDLRADRQPEFTQL
Sbjct: 182 ARDYVLPSRVNPGEWFALPQSPQLFKQILMVSGLDRYYQIARCFRDEDLRADRQPEFTQL 241

Query: 321 DMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLTYAEAMSRYGSDKPDTSFDLQ 380
           DME++F + E++++LNE L+  +F  VKGV+LP PFPRLTYAEAM RYG DKPDT +DLQ
Sbjct: 242 DMEMSFMSEEEIIELNEKLVSYIFKTVKGVELPLPFPRLTYAEAMERYGCDKPDTRYDLQ 301

Query: 381 LKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTSLKK-GDIYSEAIKSGAKG 440
           L +VSD+  DS FKVF DA+++GGI+K+L +P+G ++ SN  +K  GD++ EA ++GAKG
Sbjct: 302 LVNVSDVMKDSGFKVFRDAVANGGIVKILPIPNGNEQISNVRIKPGGDLFREASEAGAKG 361

Query: 441 LPFLKVLDDGNL 452
           L +++V +DG +
Sbjct: 362 LAYIRVREDGEI 371

BLAST of Carg08101 vs. Swiss-Prot
Match: sp|Q3MGL3|SYDND_ANAVT (Aspartate--tRNA(Asp/Asn) ligase OS=Anabaena variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=aspS PE=3 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 8.0e-127
Identity = 219/372 (58.87%), Postives = 292/372 (78.49%), Query Frame = 0

Query: 81  RTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSVI 140
           RT +CGEL   D+G+ V L GWV   R HGG+ FL+LRD SGIVQV + P   PD++ + 
Sbjct: 21  RTHYCGELRQKDIGETVTLYGWVDRRRDHGGVIFLDLRDRSGIVQVVSDPQRTPDSYELA 80

Query: 141 NDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILNSVGSKLPFLVTIADDAK 200
           N LR EYVV I G V  RP++S+N ++ TG +E+ A+ +++LN V  +LPF V+ AD   
Sbjct: 81  NSLRNEYVVEITGRVTQRPEESLNSRIPTGEVEIYADKIELLNGVRKQLPFQVSTAD--T 140

Query: 201 DSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLEDAHDFVEIETPILSRSTPEG 260
           ++ +E++RL+YRYLDLRR +M  NI LRH+VVK +RRYLED   F+E+ETPIL+RSTPEG
Sbjct: 141 ETVREDLRLKYRYLDLRRDRMARNIQLRHQVVKAMRRYLEDVEGFIEVETPILTRSTPEG 200

Query: 261 ARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARCFRDEDLRADRQPEFTQL 320
           ARDY++PSRV PG ++ALPQSPQLFKQ+LMVSG D+YYQ+ARCFRDEDLRADRQPEFTQL
Sbjct: 201 ARDYVLPSRVNPGEWFALPQSPQLFKQILMVSGMDRYYQIARCFRDEDLRADRQPEFTQL 260

Query: 321 DMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLTYAEAMSRYGSDKPDTSFDLQ 380
           DME++F + E++++LNE L+  +F  VKGV+LP PFPRLTYAEAM RYG DKPDT +DLQ
Sbjct: 261 DMEMSFMSEEEIIQLNEKLVSYIFKTVKGVELPLPFPRLTYAEAMERYGCDKPDTRYDLQ 320

Query: 381 LKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTSLKK-GDIYSEAIKSGAKG 440
           L +VSD+  DS FKVF DA+++GGI+K+L +P+G ++ SN  +K  GD++ EA ++GAKG
Sbjct: 321 LVNVSDVMKDSGFKVFRDAVANGGIVKILPIPNGNEQISNVRIKPGGDLFREASEAGAKG 380

Query: 441 LPFLKVLDDGNL 452
           L +++V +DG +
Sbjct: 381 LAYIRVREDGEI 390

BLAST of Carg08101 vs. Swiss-Prot
Match: sp|Q112K0|SYDND_TRIEI (Aspartate--tRNA(Asp/Asn) ligase OS=Trichodesmium erythraeum (strain IMS101) OX=203124 GN=aspS PE=3 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 1.0e-126
Identity = 216/372 (58.06%), Postives = 291/372 (78.23%), Query Frame = 0

Query: 81  RTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSVI 140
           RT +CG++++ ++ + V LCGWV   R HGG+ FL+LRD SG++Q+ + P   PD++ + 
Sbjct: 2   RTHYCGQVNSNNIEETVNLCGWVDRRRDHGGVIFLDLRDRSGLIQIVSDPERTPDSYQIA 61

Query: 141 NDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILNSVGSKLPFLVTIADDAK 200
            D+R EYVV I G V  RP++S+N K+ TG IE+ A+ +Q+LN +G +LPF V+ A+   
Sbjct: 62  GDIRNEYVVQITGRVSRRPEESLNPKLPTGEIEIYADDIQLLNGLGKQLPFQVSTAE--T 121

Query: 201 DSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLEDAHDFVEIETPILSRSTPEG 260
           ++ +EE+RL+YRYLDLRR++M  N++LRH+V+K IRR+LED  +FVEIETPIL+RSTPEG
Sbjct: 122 EAVREELRLKYRYLDLRRERMTRNLLLRHEVIKAIRRFLEDEQNFVEIETPILTRSTPEG 181

Query: 261 ARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARCFRDEDLRADRQPEFTQL 320
           ARDYLVPSRV PG ++ALPQSPQLFKQ+LMVSGFD+YYQ+ARCFRDEDLRADRQPEFTQL
Sbjct: 182 ARDYLVPSRVHPGEWFALPQSPQLFKQILMVSGFDRYYQIARCFRDEDLRADRQPEFTQL 241

Query: 321 DMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLTYAEAMSRYGSDKPDTSFDLQ 380
           DME++F + E++L+LNE L+  +F +VKG+ LP PFPRLTY EAM RYGSDKPD  F L+
Sbjct: 242 DMEMSFMSQEEILQLNEKLVAYIFQKVKGIDLPLPFPRLTYTEAMERYGSDKPDVRFGLE 301

Query: 381 LKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTSLKK-GDIYSEAIKSGAKG 440
           L DVSD+  DS FKVF+ A++ GGI+KVL +P G    SN  +K  GD++ EA + GAKG
Sbjct: 302 LVDVSDLMKDSGFKVFSGAIAKGGIVKVLPIPGGNDAISNVRIKPGGDLFKEASEVGAKG 361

Query: 441 LPFLKVLDDGNL 452
           L +++V + G +
Sbjct: 362 LAYVRVKEGGKI 371

BLAST of Carg08101 vs. Swiss-Prot
Match: sp|B0CF01|SYDND_ACAM1 (Aspartate--tRNA(Asp/Asn) ligase OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=aspS PE=3 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 5.7e-125
Identity = 214/370 (57.84%), Postives = 288/370 (77.84%), Query Frame = 0

Query: 81  RTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSVI 140
           RT +C EL    VG+RV LCGWV   R HGG+ FL++RD +G++Q+ + P   P+A+   
Sbjct: 2   RTHYCSELRNDHVGERVTLCGWVDRRRDHGGVIFLDVRDRTGLIQIVSDPERTPEAYPQA 61

Query: 141 NDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILNSVGSKLPFLVTIADDAK 200
           + LR EYVV I G V  RPDDS+N ++ TG +E+ A+ +++LN+V  +LPF V+ AD   
Sbjct: 62  DALRNEYVVRIHGEVSRRPDDSLNPRLPTGEVEIYADQIELLNAVHQQLPFQVSTAD--V 121

Query: 201 DSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLEDAHDFVEIETPILSRSTPEG 260
           +S KEE+RL+YRYLDLRR +M+ NIM+RH++++ IRRYLED  +FV+IETP+L+RSTPEG
Sbjct: 122 ESVKEELRLKYRYLDLRRDRMHQNIMMRHRLIQSIRRYLEDQQNFVDIETPVLTRSTPEG 181

Query: 261 ARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARCFRDEDLRADRQPEFTQL 320
           ARDYLVPSR  PG ++ALPQSPQ+FKQ+LMVSGFD+YYQ+ARCFRDEDLRADRQPEFTQL
Sbjct: 182 ARDYLVPSRANPGEWFALPQSPQIFKQLLMVSGFDRYYQIARCFRDEDLRADRQPEFTQL 241

Query: 321 DMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLTYAEAMSRYGSDKPDTSFDLQ 380
           DME++F + E++++LNEDL+R VF  +K + LP  FP++TYAEAM R+G+D+PDT FDL+
Sbjct: 242 DMEMSFMSQEEIIQLNEDLMRHVFKTLKDIDLPKTFPQMTYAEAMERFGTDRPDTRFDLE 301

Query: 381 LKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTSLKK-GDIYSEAIKSGAKG 440
           L +VSD+  DS FKVF+ A+  GG +KVL +P G  + SN  +K  GD++SEA   GAKG
Sbjct: 302 LVNVSDLLEDSGFKVFSGAIKKGGQVKVLPIPDGNAKISNVRIKPGGDLFSEASAGGAKG 361

Query: 441 LPFLKVLDDG 450
           L +++V DDG
Sbjct: 362 LAYIRVKDDG 369

BLAST of Carg08101 vs. TrEMBL
Match: tr|A0A0A0KT15|A0A0A0KT15_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000540 PE=3 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 3.9e-227
Identity = 400/451 (88.69%), Postives = 417/451 (92.46%), Query Frame = 0

Query: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60
           MSRS+LRA PFST+R KPPQFLPI H ISHLR  +SIS T RP+SS S SAT  PSSATE
Sbjct: 1   MSRSLLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATE 60

Query: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
            LV +S   SP KDSLQWINRTA CGELS  DVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  ILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHS INDLRLEYVVA+EG VRSRP DS+NVKMKTGLIEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240
           ILNSVGSKLPFLVT ADDAKDSAKEEIRLRYRYLDLRRKQMN NIMLRHKVVKL+RRYLE
Sbjct: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLE 240

Query: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300
           D H FVEIETPILSRSTPEGARDYLVPSRVQPGTF+ALPQSPQLFKQMLMVSGFDKYYQ+
Sbjct: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRVQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMEL+FTT EDML+LNEDLIR+VFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSD+PDT FDLQL DVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDDGNL 452
           T+LKKGDIY+EA KSGAKGLPFLKV DDG +
Sbjct: 421 TALKKGDIYTEATKSGAKGLPFLKVTDDGGI 451

BLAST of Carg08101 vs. TrEMBL
Match: tr|A0A1S3BZT5|A0A1S3BZT5_CUCME (aspartate--tRNA ligase, chloroplastic/mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494835 PE=3 SV=1)

HSP 1 Score: 766.1 bits (1977), Expect = 4.3e-218
Identity = 386/451 (85.59%), Postives = 402/451 (89.14%), Query Frame = 0

Query: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60
           MSRS+LRA PFS +R KPPQFLPI H ISHLRL +SIS T  P+S               
Sbjct: 1   MSRSLLRAFPFSNIRPKPPQFLPILHHISHLRLPQSISTTQTPVSXXXXXXXXXXXXXXX 60

Query: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
                  S SP KDSLQWINRTA CGELS  DVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  XXXXXXXSHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHS INDLRLEYVVA+EG VRSRP DS+NVKMKTG IEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSAINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGFIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240
           ILNSVGSKLPFLVT ADDAKDSAKEEIRLRYRYLDLRRKQMN NIMLRHKVVKLIRRYLE
Sbjct: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLIRRYLE 240

Query: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300
           D H FVEIETPILSRSTPEGARDYLVPSRVQPGTF+ALPQSPQLFKQMLMVSGFDKYYQ+
Sbjct: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRVQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMEL+FTT EDML+LNEDLIR+VFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSD+PDT FDLQL DVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDDGNL 452
           T+LKKGDIY+EA+KSGAKGLPFLKV DDG +
Sbjct: 421 TALKKGDIYTEAMKSGAKGLPFLKVTDDGGI 451

BLAST of Carg08101 vs. TrEMBL
Match: tr|A0A0S3QWN3|A0A0S3QWN3_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis OX=157739 GN=Vigan.01G019300 PE=3 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 1.3e-195
Identity = 350/447 (78.30%), Postives = 394/447 (88.14%), Query Frame = 0

Query: 6   LRALPFSTLRHKPPQFLPIFHRISH-LRLAKSISRTLRPISSLSVSATHPPSSATETLVS 65
           L+ALP  T R +P   L    R+S+ + L  S +RT+  +S+ +  +T P  SA+E L S
Sbjct: 5   LKALPLMTSRTRPS--LVFLCRVSNTVLLPLSRTRTVFSVSASTTPSTQPLPSASEILTS 64

Query: 66  SSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIV 125
           ++K P  L +SL+WINRTA CGELS+ DVGKRV+LCGWVALHRVHGGLTFLNLRDHSGIV
Sbjct: 65  NAKPPLALTESLEWINRTALCGELSSNDVGKRVQLCGWVALHRVHGGLTFLNLRDHSGIV 124

Query: 126 QVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILNS 185
           QVTTLP+EFPDAHS INDLRLEYVVAI+G+VRSRP +SIN KMKTG IEVAA  VQ+LNS
Sbjct: 125 QVTTLPDEFPDAHSAINDLRLEYVVAIDGVVRSRPSESINKKMKTGSIEVAARRVQVLNS 184

Query: 186 VGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLEDAHD 245
           V SKLPFLVT ADDAKDS KEEIRLRYR LDLRR+QMN NI+LRHKVVKLIRRYLED H 
Sbjct: 185 VNSKLPFLVTTADDAKDSLKEEIRLRYRCLDLRRQQMNFNILLRHKVVKLIRRYLEDVHG 244

Query: 246 FVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARCF 305
           FVEIETPILSRSTPEGARDYLVPSR+QPGTFYALPQSPQLFKQMLMV+GFDKYYQVARCF
Sbjct: 245 FVEIETPILSRSTPEGARDYLVPSRIQPGTFYALPQSPQLFKQMLMVAGFDKYYQVARCF 304

Query: 306 RDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLTYAEA 365
           RDEDLRADRQPEFTQLDME+AFT +EDMLKLNE+LIR+VF+E+KGV+LPN FPRLTYAEA
Sbjct: 305 RDEDLRADRQPEFTQLDMEMAFTPYEDMLKLNEELIRKVFFEIKGVELPNSFPRLTYAEA 364

Query: 366 MSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTSLK 425
           MSRYGSD+PDT FDL+LKDVSDIF+ S FKVF+D+L SGG+IKV CVPSG K+YSN++LK
Sbjct: 365 MSRYGSDRPDTRFDLELKDVSDIFSGSSFKVFSDSLESGGVIKVFCVPSGTKKYSNSALK 424

Query: 426 KGDIYSEAIKSGAKGLPFLKVLDDGNL 452
           KGDIYSEA+KSGAKGLPFLKVLDDGNL
Sbjct: 425 KGDIYSEALKSGAKGLPFLKVLDDGNL 449

BLAST of Carg08101 vs. TrEMBL
Match: tr|A0A1S3ULV4|A0A1S3ULV4_VIGRR (aspartate--tRNA ligase, chloroplastic/mitochondrial OS=Vigna radiata var. radiata OX=3916 GN=LOC106766801 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 3.9e-195
Identity = 348/447 (77.85%), Postives = 393/447 (87.92%), Query Frame = 0

Query: 6   LRALPFSTLRHKPPQFLPIFHRISH-LRLAKSISRTLRPISSLSVSATHPPSSATETLVS 65
           L+ALP  T R +P   L    R+S+ + L  S +RT+  +S+ +  +  P  SA+E L S
Sbjct: 5   LKALPLMTSRTRPS--LVFLCRVSNTVLLPLSRTRTVFSVSASTTPSIQPLPSASEILTS 64

Query: 66  SSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIV 125
           ++K P  L +SL+WINRTA CGELS+ DVGKRV+LCGWVALHRVHGGLTF NLRDHSGIV
Sbjct: 65  NAKPPLALTESLEWINRTALCGELSSNDVGKRVQLCGWVALHRVHGGLTFFNLRDHSGIV 124

Query: 126 QVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILNS 185
           QVTTLP+EFPDAHS INDLRLEYVVAI+G+VRSRP +SIN KMKTG IEVAA  VQ+LNS
Sbjct: 125 QVTTLPDEFPDAHSAINDLRLEYVVAIDGVVRSRPSESINKKMKTGSIEVAARRVQVLNS 184

Query: 186 VGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLEDAHD 245
           V SKLPFLVT ADDAKDS KEEIRLRYR LDLRR+QMN NI+LRHKVVKLIRRYLED H 
Sbjct: 185 VNSKLPFLVTTADDAKDSLKEEIRLRYRCLDLRRQQMNFNILLRHKVVKLIRRYLEDVHG 244

Query: 246 FVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARCF 305
           FVEIETPILSRSTPEGARDYLVPSR+QPGTFYALPQSPQLFKQMLMV+GFDKYYQVARCF
Sbjct: 245 FVEIETPILSRSTPEGARDYLVPSRIQPGTFYALPQSPQLFKQMLMVAGFDKYYQVARCF 304

Query: 306 RDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLTYAEA 365
           RDEDLRADRQPEFTQLDME+AFT +EDMLKLNE+LIR+VF+E+KGV+LPNPFPRLTYAEA
Sbjct: 305 RDEDLRADRQPEFTQLDMEMAFTPYEDMLKLNEELIRKVFFEIKGVELPNPFPRLTYAEA 364

Query: 366 MSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTSLK 425
           MSRYGSD+PDT FDL+LKDVSDIF+ S FKVF+D+L SGG+IKV CVPSG K+YSN++LK
Sbjct: 365 MSRYGSDRPDTRFDLELKDVSDIFSGSSFKVFSDSLESGGVIKVFCVPSGTKKYSNSALK 424

Query: 426 KGDIYSEAIKSGAKGLPFLKVLDDGNL 452
           KGDIYSEA+KSGAKGLPFLKVLDDGN+
Sbjct: 425 KGDIYSEALKSGAKGLPFLKVLDDGNM 449

BLAST of Carg08101 vs. TrEMBL
Match: tr|A0A067KNA5|A0A067KNA5_JATCU (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_06970 PE=3 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 5.1e-195
Identity = 346/447 (77.40%), Postives = 391/447 (87.47%), Query Frame = 0

Query: 5   ILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATETLVS 64
           +L++LP  T   KP   LP+ H I      K+IS++ +   S+S S T  PS +TE L+ 
Sbjct: 4   LLKSLPLITFLSKPS--LPLLHTIFKHHFPKTISKSTQRFFSVSAS-TSSPSPSTEALL- 63

Query: 65  SSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIV 124
               P   K+SL W++RT FCG+LS  DVGKRVRLCGWVALHRVHGGLTFLNLRDH+GIV
Sbjct: 64  -KPQPVSFKESLDWVSRTGFCGKLSVNDVGKRVRLCGWVALHRVHGGLTFLNLRDHTGIV 123

Query: 125 QVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILNS 184
           QVTTLP+EFPDAHS INDLRLEYVVAIEG++RSRP +S+N KMKTGLIE+AAE+VQ+LN+
Sbjct: 124 QVTTLPDEFPDAHSTINDLRLEYVVAIEGVIRSRPSESVNKKMKTGLIELAAENVQVLNA 183

Query: 185 VGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLEDAHD 244
           V SKLPFLVT ADDAKDS KEEIRLR+R LDLRR+QMN N+MLRH+VVKLIRRYLED + 
Sbjct: 184 VRSKLPFLVTTADDAKDSVKEEIRLRFRCLDLRRQQMNFNLMLRHRVVKLIRRYLEDVYG 243

Query: 245 FVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARCF 304
           FVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQ+ARCF
Sbjct: 244 FVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQIARCF 303

Query: 305 RDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLTYAEA 364
           RDEDLRADRQPEFTQLDMELAFT  EDML+LNEDLIR+VF E+KGV+LPNPFPRLTYAEA
Sbjct: 304 RDEDLRADRQPEFTQLDMELAFTPLEDMLRLNEDLIRKVFLEIKGVELPNPFPRLTYAEA 363

Query: 365 MSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTSLK 424
           MSRYGSD+PDT FDL+LKDVSDIF +SPF+VFAD+L +GGI+KVLCVPSGAK YSNT+LK
Sbjct: 364 MSRYGSDRPDTRFDLELKDVSDIFVESPFRVFADSLKNGGILKVLCVPSGAKTYSNTALK 423

Query: 425 KGDIYSEAIKSGAKGLPFLKVLDDGNL 452
           KGDIY+EAIKSGAKGLPFLKVLDDG +
Sbjct: 424 KGDIYNEAIKSGAKGLPFLKVLDDGEI 445

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022942888.13.4e-25199.33aspartate--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 [Cucurbita m... [more]
XP_023554090.13.8e-25099.11aspartate--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 [Cucurbita p... [more]
XP_022975696.12.1e-24898.44aspartate--tRNA ligase, chloroplastic/mitochondrial-like [Cucurbita maxima] >XP_... [more]
XP_004150278.25.8e-22788.69PREDICTED: aspartate--tRNA ligase, mitochondrial [Cucumis sativus] >KGN52733.1 h... [more]
XP_023551993.19.3e-22588.47aspartate--tRNA ligase, chloroplastic/mitochondrial-like [Cucurbita pepo subsp. ... [more]
Match NameE-valueIdentityDescription
AT4G33760.11.8e-17769.87tRNA synthetase class II (D, K and N) family protein[more]
AT3G13490.17.3e-1424.55Lysyl-tRNA synthetase, class II[more]
AT3G11710.14.4e-1126.03lysyl-tRNA synthetase 1[more]
Match NameE-valueIdentityDescription
sp|F4JJT9|SYDM_ARATH3.2e-17669.87Aspartate--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3... [more]
sp|Q8YUB6|SYDND_NOSS14.7e-12758.87Aspartate--tRNA(Asp/Asn) ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTE... [more]
sp|Q3MGL3|SYDND_ANAVT8.0e-12758.87Aspartate--tRNA(Asp/Asn) ligase OS=Anabaena variabilis (strain ATCC 29413 / PCC ... [more]
sp|Q112K0|SYDND_TRIEI1.0e-12658.06Aspartate--tRNA(Asp/Asn) ligase OS=Trichodesmium erythraeum (strain IMS101) OX=2... [more]
sp|B0CF01|SYDND_ACAM15.7e-12557.84Aspartate--tRNA(Asp/Asn) ligase OS=Acaryochloris marina (strain MBIC 11017) OX=3... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0KT15|A0A0A0KT15_CUCSA3.9e-22788.69Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000540 PE=3 SV=1[more]
tr|A0A1S3BZT5|A0A1S3BZT5_CUCME4.3e-21885.59aspartate--tRNA ligase, chloroplastic/mitochondrial isoform X1 OS=Cucumis melo O... [more]
tr|A0A0S3QWN3|A0A0S3QWN3_PHAAN1.3e-19578.30Uncharacterized protein OS=Vigna angularis var. angularis OX=157739 GN=Vigan.01G... [more]
tr|A0A1S3ULV4|A0A1S3ULV4_VIGRR3.9e-19577.85aspartate--tRNA ligase, chloroplastic/mitochondrial OS=Vigna radiata var. radiat... [more]
tr|A0A067KNA5|A0A067KNA5_JATCU5.1e-19577.40Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_06970 PE=3 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
Vocabulary: Molecular Function
TermDefinition
GO:0016874ligase activity
GO:0003676nucleic acid binding
GO:0005524ATP binding
GO:0004812aminoacyl-tRNA ligase activity
GO:0000166nucleotide binding
Vocabulary: Biological Process
TermDefinition
GO:0006418tRNA aminoacylation for protein translation
Vocabulary: INTERPRO
TermDefinition
IPR004115GAD-like_sf
IPR012340NA-bd_OB-fold
IPR006195aa-tRNA-synth_II
IPR004524Asp-tRNA-ligase_1
IPR004365NA-bd_OB_tRNA
IPR029351GAD_dom
IPR004364aa-tRNA-synt_II
IPR002312Asp/Asn-tRNA-synth_IIb
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0016874 ligase activity
molecular_function GO:0000166 nucleotide binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08101-RACarg08101-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002312Aspartyl/Asparaginyl-tRNA synthetase, class IIbPRINTSPR01042TRNASYNTHASPcoord: 295..308
score: 49.51
coord: 278..290
score: 65.38
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 205..380
e-value: 1.2E-64
score: 218.2
IPR029351GAD domainPFAMPF02938GADcoord: 396..449
e-value: 2.0E-9
score: 37.5
NoneNo IPR availableGENE3DG3DSA:2.40.50.140coord: 72..186
e-value: 1.2E-32
score: 114.2
NoneNo IPR availableGENE3DG3DSA:3.30.930.10coord: 197..402
e-value: 1.4E-56
score: 193.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 47..70
NoneNo IPR availablePANTHERPTHR22594:SF43SUBFAMILY NOT NAMEDcoord: 81..452
NoneNo IPR availablePANTHERPTHR22594ASPARTYL/LYSYL-TRNA SYNTHETASEcoord: 81..452
NoneNo IPR availableCDDcd04317EcAspRS_like_Ncoord: 81..221
e-value: 1.35205E-59
score: 192.736
NoneNo IPR availableSUPERFAMILYSSF55681Class II aaRS and biotin synthetasescoord: 205..398
IPR004365OB-fold nucleic acid binding domain, AA-tRNA synthetase-typePFAMPF01336tRNA_anti-codoncoord: 97..179
e-value: 9.6E-12
score: 44.6
IPR004524Aspartate-tRNA ligase, type 1TIGRFAMTIGR00459TIGR00459coord: 80..449
e-value: 2.9E-141
score: 469.7
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 227..381
score: 13.525
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILYSSF50249Nucleic acid-binding proteinscoord: 81..183
IPR004115GAD-like domain superfamilySUPERFAMILYSSF55261GAD domain-likecoord: 377..449

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Carg08101CmaCh16G002990Cucurbita maxima (Rimu)carcmaB0526
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Carg08101Wax gourdcarwgoB0456
Carg08101Silver-seed gourdcarcarB324
Carg08101Silver-seed gourdcarcarB401
Carg08101Silver-seed gourdcarcarB402
Carg08101Silver-seed gourdcarcarB404
Carg08101Cucumber (Gy14) v2carcgybB0383
Carg08101Cucumber (Gy14) v2carcgybB0388
Carg08101Cucumber (Gy14) v2carcgybB0389
Carg08101Cucumber (Gy14) v1carcgyB0433
Carg08101Cucumber (Gy14) v1carcgyB0439
Carg08101Cucurbita maxima (Rimu)carcmaB0528
Carg08101Cucurbita maxima (Rimu)carcmaB0525
Carg08101Cucurbita maxima (Rimu)carcmaB0529
Carg08101Cucurbita moschata (Rifu)carcmoB0508
Carg08101Cucurbita moschata (Rifu)carcmoB0509
Carg08101Cucurbita moschata (Rifu)carcmoB0514
Carg08101Cucurbita moschata (Rifu)carcmoB0516
Carg08101Cucurbita pepo (Zucchini)carcpeB0523
Carg08101Cucurbita pepo (Zucchini)carcpeB0526
Carg08101Cucurbita pepo (Zucchini)carcpeB0527
Carg08101Cucurbita pepo (Zucchini)carcpeB0529
Carg08101Wild cucumber (PI 183967)carcpiB0407
Carg08101Cucumber (Chinese Long) v2carcuB0402
Carg08101Cucumber (Chinese Long) v2carcuB0409
Carg08101Cucumber (Chinese Long) v2carcuB0410
Carg08101Cucumber (Chinese Long) v3carcucB0406
Carg08101Bottle gourd (USVL1VR-Ls)carlsiB340
Carg08101Bottle gourd (USVL1VR-Ls)carlsiB341
Carg08101Melon (DHL92) v3.5.1carmeB0383
Carg08101Melon (DHL92) v3.5.1carmeB0386
Carg08101Melon (DHL92) v3.6.1carmedB0387
Carg08101Melon (DHL92) v3.6.1carmedB0390
Carg08101Watermelon (Charleston Gray)carwcgB0365
Carg08101Watermelon (Charleston Gray)carwcgB0368
Carg08101Watermelon (97103) v1carwmB0366
Carg08101Watermelon (97103) v1carwmB0375
Carg08101Watermelon (97103) v2carwmbB0391
Carg08101Watermelon (97103) v2carwmbB0394